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N-carbamylglutamate restores nitric oxide synthesis and attenuates high altitudeinduced pulmonary hypertension in Holstein heifers ascended to high altitude 预览
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作者 Shuxiang Wang Arash Azarfar +2 位作者 Yajing Wang Zhijun Cao Shengli Li 《畜牧与生物技术杂志:英文版》 SCIE CAS CSCD 2018年第4期1000-1007,共8页
Background:High-altitude pulmonary hypertension(HAPH)is a life-threating condition for animals in high altitude,and disturbance of endothelial nitric oxide(NO)synthesis contributes to its pathogenesis.N-carbamylglutam... Background:High-altitude pulmonary hypertension(HAPH)is a life-threating condition for animals in high altitude,and disturbance of endothelial nitric oxide(NO)synthesis contributes to its pathogenesis.N-carbamylglutamate(NCG),which enhances arginine synthesis,promotes endogenous synthesis of NO.In this study,we determined the effects of NCG on alleviating HAPH in Holstein heifers that ascended to Tibet(Lhasa,3,658 m).Methods:Exp.1,2,000 Holstein heifers were transported from low elevation(1,027 m)to Lhasa.After being exposed to hypoxia for 1 yr,Holstein heifers were assigned to a healthy group(Control,n=6)with mean pulmonary hypertension(mPAP)<41 mmHg,and an HAPH affected group(HAPH,n=6)with mPAP >49 mmHg.Lung tissues were collected to evaluate histopathological changes and the expression of endothelial nitric oxide synthase(eNOS).Exp.2,ten healthy heifers and 10 HAPH affected heifers were supplemented with NCG(20 g/d per heifer)for 4 wk.Physiological parameters were determined and blood samples were collected on d?1 and d 28 of the feeding trial.Results:Expression of eNOS in small pulmonary arteriole intima was higher in the healthy than HAPH group(P=0.006),whereas HAPH group had significantly thicker media and adventitia than healthy group(all P<0.05).The mRNA of eNOS and protein level of eNOS were higher in the lungs of heifers in the healthy group than in the HAPH group(both P<0.001),whereas endothelin-1 protein levels were higher in HAPH group than in the healthy group(P=0.025).NCG supplementation decreased mPAP and ammonia(both P=0.001),whereas it increased the expression of eNOS,arginine,and plasma NO(all P<0.05).Conclusions:The expression of eNOS was decreased in Holstein heifers with HAPH.NCG supplementation decreased mPAP through the restoration of eNOS and endogenous NO synthesis. 展开更多
关键词 HIGH-ALTITUDE pulmonary HYPERTENSION HOLSTEIN HEIFERS N-carbamylglutamate TIBET
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Comparison of rumen bacteria distribution in original rumen digesta, rumen liquid and solid fractions in lactating Holstein cows 预览
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作者 Shoukun Ji Hongtao Zhang +6 位作者 Hui Yan Arash Azarfar Haitao Shi Gibson Alugongo Shengli Li Zhijun Cao Yajing Wang 《畜牧与生物技术杂志:英文版》 SCIE CAS CSCD 2017年第3期636-642,共7页
Background: Original rumen digesta, rumen liquid and solid fractions have been frequently used to assess the rumen bacterial community. However, bacterial profiles in rumen original digesta, liquid and solid fractions... Background: Original rumen digesta, rumen liquid and solid fractions have been frequently used to assess the rumen bacterial community. However, bacterial profiles in rumen original digesta, liquid and solid fractions vary from each other and need to be better established.Methods: To compare bacterial profiles in each fraction, samples of rumen digesta from six cows fed either a high fiber diet(HFD) or a high energy diet(HED) were collected via rumen fistulas. Rumen digesta was then squeezed through four layers of cheesecloth to separate liquid and solid fractions. The bacterial profiles of rumen original digesta, liquid and solid fractions were analyzed with High-throughput sequencing technique.Results: Rumen bacterial diversity was mainly affected by diet and individual cow(P > 0.05) rather than rumen fraction. Bias distributed bacteria were observed in solid and liquid fractions of rumen content using Venn diagram and LEf Se analysis. Fifteen out of 16 detected biomarkers(using LEf Se analysis) were found in liquid fraction, and these 15 biomarkers contributed the most to the bacterial differences among rumen content fractions.Conclusions: Similar results were found when using samples of original rumen digesta, rumen liquid or solid fractions to assess diversity of rumen bacteria; however, more attention should be draw onto bias distributed bacteria in different ruminal fractions, especially when liquid fraction has been used as a representative sample for rumen bacterial study. 展开更多
关键词 瘤胃细菌 液体 组分 固体 奶牛 细菌多样性 生物标志物 瘤胃内容物
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