In this study, a nanobiosensor for detecting explosives was developed, in which the peptide was synthesized with trinitrotoluene(TNT)-specific sequence and immobilized on nanodevice by Au–S covalent linkage, and th...In this study, a nanobiosensor for detecting explosives was developed, in which the peptide was synthesized with trinitrotoluene(TNT)-specific sequence and immobilized on nanodevice by Au–S covalent linkage, and the nanocup arrays were fabricated by nanoimprint and deposited with Au nanoparticles to generate localized surface plasmon resonance(LSPR). The device was used to monitor slight change from specific binding of 2,4,6-TNT to the peptide. With high refractive index sensing of 104nm/RIU, the nanocup device can detect the binding of TNT at concentration as low as3.12×10-7mg mL-1 by optical transmission spectrum modulated by LSPR. The nanosensor is also able to distinguish TNT from analogs of 2,4-dinitrotoluene and 3-nitrotoluene in the mixture with great selectivity. The peptide-based nanosensor provides novel approaches to design versatile biosensor assays by LSPR for chemical molecules.展开更多
AIM To investigate the role of single nucleotidepolymorphisms (SNPs) in CD24 gene in susceptibilityand overall survival of gastric cancer (GC).METHODS: We genotyped 3 tagging SNPs ofCD24 -P-534 in the promoter re...AIM To investigate the role of single nucleotidepolymorphisms (SNPs) in CD24 gene in susceptibilityand overall survival of gastric cancer (GC).METHODS: We genotyped 3 tagging SNPs ofCD24 -P-534 in the promoter region, P170 in the codingregion of exon 2 and P1527 in the 3′ untranslatedregion - using polymerase chain reaction-restrictionfragment length polymorphism in specimens from 679histologically-confirmed GC cases, 111 gastric atrophy(GA) cases and 976 tumor-free controls. Serum immunoglobulin G antibodies to Helicobacter pylori (H.pylori ) of all subjects were detected by enzyme-linkedimmunosorbent assay. CD24 expression was evaluatedby immunohistochemistry in 131 GC specimens.Correlations between SNPs and risk of GC or GA wereshown by P values and odd ratios (ORs) with 95%confidence intervals (95%CI) compared with the mostcommon genotype of each SNP using the unconditionallogistic regression model after adjusting for age, sexand H. pylori infection. Survival within each SNP groupwas plotted by Kaplan-Meier method and comparedby log-rank test (recessive model). Hazard ratios with95%CIs were computed by Cox regression modelafter adjusting for age, sex, histological type, tumordifferentiation, clinical stage and post-operationalchemotherapy.RESULTS: All of the three loci were in Hardy-Weinbergequilibrium in the control group. Median followuptime for the 600 GC patients included in thesurvival analysis was 36.2 mo (range, 2.1-66.7 mo;95%CI: 34.3-36.5 mo). Patients with the P-534 A/Agenotype had significantly shorter survival (HR =1.38, 95%CI: 1.01-1.88, P = 0.042) than did theC/C or C/A genotype carriers after adjusting for age,sex, histological type, tumor differentiation, clinicalstage and post-operational chemotherapy. Thistrend was more evident in patients who lived longerthan 2.5 years (HR = 7.55, 95%CI: 2.16-26.32, P =0.001). The P170 T/T genotype was associated witha shorter lifespan than the non-T/T genotypes, butnot significantly so. None of the three 展开更多
基金Acknowledgements We thank Dr. Tan Meilian, Dr. Olanike Omolehin, Dr. Gao Guoqing and the anonymous reviewers for their helpful comments. This project was supported by the Guangxi Academy of Agricultural Sciences Foundation, China (2015JZ08 and 2015YT57), the Guangxi Sciences Foundation, China (2011GXNSFA018079), the Modern Agro-industry Technology Research System, China (CARS-14-19) and the National Natural Science Foundation of China (31160294 and 31240059).
基金Projects (51301082, 51464031) supported by the National Natural Science Foundation of China Project (2015011038) supported by theNatural Science Foundation of Shanxi Province, China
基金supported by the National Natural Science Foundation of China(Grant No.81371643)the Zhejiang Provincial Natural Science Foundation of China for Distinguished Young Scholars(Grant No.LR13H180002)
文摘In this study, a nanobiosensor for detecting explosives was developed, in which the peptide was synthesized with trinitrotoluene(TNT)-specific sequence and immobilized on nanodevice by Au–S covalent linkage, and the nanocup arrays were fabricated by nanoimprint and deposited with Au nanoparticles to generate localized surface plasmon resonance(LSPR). The device was used to monitor slight change from specific binding of 2,4,6-TNT to the peptide. With high refractive index sensing of 104nm/RIU, the nanocup device can detect the binding of TNT at concentration as low as3.12×10-7mg mL-1 by optical transmission spectrum modulated by LSPR. The nanosensor is also able to distinguish TNT from analogs of 2,4-dinitrotoluene and 3-nitrotoluene in the mixture with great selectivity. The peptide-based nanosensor provides novel approaches to design versatile biosensor assays by LSPR for chemical molecules.
基金National Natural Science Foundation of China,No.81373084 and No.81273065+2 种基金
the Norman Bethune Program of Jilin Universit,No.2013025
the Youth Fund of the First Hospital of Jilin University,No.JDYY42013014
文摘AIM To investigate the role of single nucleotidepolymorphisms (SNPs) in CD24 gene in susceptibilityand overall survival of gastric cancer (GC).METHODS: We genotyped 3 tagging SNPs ofCD24 -P-534 in the promoter region, P170 in the codingregion of exon 2 and P1527 in the 3′ untranslatedregion - using polymerase chain reaction-restrictionfragment length polymorphism in specimens from 679histologically-confirmed GC cases, 111 gastric atrophy(GA) cases and 976 tumor-free controls. Serum immunoglobulin G antibodies to Helicobacter pylori (H.pylori ) of all subjects were detected by enzyme-linkedimmunosorbent assay. CD24 expression was evaluatedby immunohistochemistry in 131 GC specimens.Correlations between SNPs and risk of GC or GA wereshown by P values and odd ratios (ORs) with 95%confidence intervals (95%CI) compared with the mostcommon genotype of each SNP using the unconditionallogistic regression model after adjusting for age, sexand H. pylori infection. Survival within each SNP groupwas plotted by Kaplan-Meier method and comparedby log-rank test (recessive model). Hazard ratios with95%CIs were computed by Cox regression modelafter adjusting for age, sex, histological type, tumordifferentiation, clinical stage and post-operationalchemotherapy.RESULTS: All of the three loci were in Hardy-Weinbergequilibrium in the control group. Median followuptime for the 600 GC patients included in thesurvival analysis was 36.2 mo (range, 2.1-66.7 mo;95%CI: 34.3-36.5 mo). Patients with the P-534 A/Agenotype had significantly shorter survival (HR =1.38, 95%CI: 1.01-1.88, P = 0.042) than did theC/C or C/A genotype carriers after adjusting for age,sex, histological type, tumor differentiation, clinicalstage and post-operational chemotherapy. Thistrend was more evident in patients who lived longerthan 2.5 years (HR = 7.55, 95%CI: 2.16-26.32, P =0.001). The P170 T/T genotype was associated witha shorter lifespan than the non-T/T genotypes, butnot significantly so. None of the three
基金Project supported by the National Natural Science Foundation of China (Nos. 31372054 and 30971999) and the State Key Laboratory of Plant Physiology and Biochemistry Open Project of China (No. SKLPPBKF 1404)