期刊文献+
共找到81篇文章
< 1 2 5 >
每页显示 20 50 100
Research on the Model of Long Term Generation Planning in Power Market Reform 预览
1
作者 Bin Han Xiao-Lei Li +2 位作者 Jing-Hua Yan Hui Cui Zhi-Qiang Luo 《能源与动力工程(英文)》 2017年第4期525-534,共10页
With the deepening of China’s power market, bilateral transactions will continue to grow in large scale. The release of bilateral transactions locked more regulatory resources of the power grid, will directly affect ... With the deepening of China’s power market, bilateral transactions will continue to grow in large scale. The release of bilateral transactions locked more regulatory resources of the power grid, will directly affect the operation mode of the unit and the implementation of planned electricity. In the paper, considering the large-scale bilateral trade effect on the peak regulation of power grid, energy saving and emission reduction, power system security and other factors, and then putting forward the method of long term generation planning and annual planning model to adapt to the safe operation of power grid in China. In the model, the target is minimizing the monthly load rate deviation and the annual electric quantity deviation rate, the latter includes the capacity factor. In addition, the constraints include the monthly quantity of electricity, adjustable utilization rate deviation, load rate, reserve and key sections, etc. Through an example to verify the correctness of the model, the planning and power transaction results can satisfy the peak regulation of load, energy saving and emission reduction and safety operation of the power grid requirements. 展开更多
关键词 POWER MARKET Generation SCHEDULING PEAK Regulation Energy SAVING and EMISSION Reduction POWER System Security
在线阅读 免费下载
Oncogenic potential of IDH1R132C mutant incholangiocarcinoma development in mice 预览
2
作者 Ding, Ning Che, Li +6 位作者 Li, Xiao-Lei Liu, Yan Jiang, Li-Jie Fan, Biao Tao, Jun-Yan Chen, Xin Ji, Jia-Fu 《世界胃肠病学杂志:英文版》 SCIE CAS 2016年第6期2071-2080,共10页
AIM To investigate whether IDH1R132C mutant incombination with loss of p53 and activated Notchsignaling promotes intrahepatic cholangiocarcinoma(ICC) development.METHODS: We applied hydrodynamic injection andsleepi... AIM To investigate whether IDH1R132C mutant incombination with loss of p53 and activated Notchsignaling promotes intrahepatic cholangiocarcinoma(ICC) development.METHODS: We applied hydrodynamic injection andsleeping beauty mediated somatic integration toinduce loss of p53 (via shP53), activation of Notch[via intracellular domain of Notch1 (NICD)] and/oroverexpression of IDH1R132C mutant together withthe sleeping beauty transposase into the mouseliver. Specifically, we co-expressed shP53 and NICD(shP53/NICD, n = 4), shP53 and IDH1R132C (shP53/IDH1R132C, n = 3), NICD and IDH1R132C (NICD/IDH1R132C, n = 4), as well as NICD, shP53 andIDH1R132C (NICD/shP53/IDH1R132C, n = 9) in mice.Mice were monitored for liver tumor development andeuthanized at various time points. Liver histology wasanalyzed by hematoxylin and eosin staining. Molecularfeatures of NICD/shP53/IDH1R132C ICC tumor cellswere characterized by Myc tag, Flag tag, Ki-67, p-Erkand p-AKT immunohistochemical staining. Desmoplastic reaction in tumor tissues was studied by Picro-Siriusred staining.RESULTS: We found that co-expression of shP53/NICD, shP53/IDH1R132C or NICD/IDH1R132C did notlead to liver tumor formation. In striking contrast, coexpressionof NICD/shP53/IDH1R132C resulted in ICCdevelopment in mice (P 〈 0.01). The tumors couldbe identified as early as 12 wk post hydrodynamicinjection. Tumors rapidly progressed, and by 18 wk posthydrodynamic injection, multiple cystic lesions could beidentified on the liver surface. NICD/shP53/IDH1R132Cliver tumors shared multiple histological features ofhuman ICCs, including hyperplasia of irregular glands.Importantly, all tumor cells were positive for thebiliary epithelial cell marker cytokeratin 19. Extensivecollagen fibers could be visualized in tumor tissuesusing Sirus red staining, duplicating the desmoplasticreaction observed in human ICC. Tumors were highlyproliferative and expressed ectopically injected genes.Together these studies supported that NICD/shP53/IDH1R132C liver 展开更多
关键词 IDH1 MUTANT NOTCH PATHWAY Intrahepaticcholangiocarcinoma MOUSE LIVER cancer p53
在线阅读 免费下载
Comparison of cosmological models using standard rulers and candles
3
作者 Xiao-Lei Li Shuo Cao +2 位作者 Xiao-Gang Zheng Song L1 Marek Biesiada 《天文和天体物理学研究:英文版》 SCIE CAS CSCD 2016年第5期125-134,共10页
In this paper, we used standard rulers and standard candles(separately and jointly) to explore five popular dark energy models under the assumption of the spatial flatness of the Universe. As standard rulers, we used ... In this paper, we used standard rulers and standard candles(separately and jointly) to explore five popular dark energy models under the assumption of the spatial flatness of the Universe. As standard rulers, we used a data set comprised of 118 galactic scale strong lensing systems(individual standard rulers if properly calibrated for the mass density profile) combined with BAO diagnostics(statistical standard ruler). Type Ia supernovae served as standard candles. Unlike most previous statistical studies involving strong lensing systems, we relaxed the assumption of a singular isothermal sphere(SIS) in favor of its generalization: the power-law mass density profile. Therefore, along with cosmological model parameters,we fitted the power law index and its first derivative with respect to the redshift(thus allowing for mass density profile evolution). It turned out that the best fitted γ parameters are in agreement with each other,irrespective of the cosmological model considered. This demonstrates that galactic strong lensing systems may provide a complementary probe to test the properties of dark energy. The fits for cosmological model parameters which we obtained are in agreement with alternative studies performed by other researchers.Because standard rulers and standard candles have different parameter degeneracies, a combination of standard rulers and standard candles gives much more restrictive results for cosmological parameters. Finally,we attempted an analysis based on model selection using information theoretic criteria(AIC and BIC). Our results support the claim that the cosmological constant model is still best and there is no(at least statistical)reason to prefer any other more complex model. 展开更多
关键词 宇宙学模型 使用标准 蜡烛 透镜系统 IA型超新星 密度分布 幂律指数 拟合参数
Effects of Processing Variables on Microstructure and Yield Ratio of High Strength Constructional Steels
4
作者 Jian KANG Cheng-ning LI +3 位作者 Xiao-lei LI Jin-hua ZHAO Guo YUAN Guo-dong WANG 《钢铁研究学报:英文版》 SCIE CAS CSCD 2016年第8期815-821,共7页
The process of"controlled rolling+relaxation+ultra fast cooling(UFC)"for high strength constructional steel with low yield ratio was presented.Microstructure and corresponding relationship with low yield rat... The process of"controlled rolling+relaxation+ultra fast cooling(UFC)"for high strength constructional steel with low yield ratio was presented.Microstructure and corresponding relationship with low yield ratio were investigated.The results showed that the constructional steels with multiphase microstructure of bainitic ferrite,martensite-austenite(M-A)and lath bainite were obtained through the creative process.The grain size decreased with the decrease in finish rolling temperature,which enhanced the strength by the grain refinement strengthening.The proper relaxation treatment promoted the bainitic ferrite lath width and the formation of blocky M-A constituent.In addition,both the tensile and yield strength increased with the decrease in finish rolling temperature and UFC final temperature,but the yield strength increased more significantly than tensile strength,which caused the increase in yield ratio.By using the process of"controlled rolling+relaxation+ultra fast cooling",the excellent comprehensive mechanical properties of 780 MPa grade constructional steels of 12-40 mm in thickness were achieved. 展开更多
关键词 高强度结构钢 屈服强度 显微组织 工艺参数 超快速冷却 终轧温度 松弛处理 综合机械性能
An Actively Mode-Locked Ho:YAG Solid-Laser Pumped by a Tm-Doped Fiber Laser
5
作者 姚宝权 李贺 +7 位作者 李晓磊 陈毅 段小明 白爽 杨宏宇 崔铮 申英杰 戴通宇 《中国物理快报:英文版》 SCIE CAS CSCD 2016年第4期44-47,共4页
An actively mode-locked Ho:YAG laser pumped by a diode-pumped Tm-doped fiber laser is reported.For the cw operation,we obtain the maximum output power of 3.43 W with a central wavelength 2022.2 nm at the maximum incid... An actively mode-locked Ho:YAG laser pumped by a diode-pumped Tm-doped fiber laser is reported.For the cw operation,we obtain the maximum output power of 3.43 W with a central wavelength 2022.2 nm at the maximum incident pump power of 11.4 W,corresponding to a slope efficiency of 34.5%.The beam quality factor M2 is 1.16,and the output beam is close to fundamental TEM_(00) In the case of the CWML operation,a stable pulse train is generated with an average output power up to 3.41 W with a slope efficiency of 34.3%at the incident pump power of 11.4 W and a pulse duration of 294 ps at a repetition rate of 81.92 MHz.In addition,the maximum single pulse energy is 41.6 n J. 展开更多
关键词 主动锁模光纤激光器 激光泵浦 YAG 最大输出功率 激光二极管泵浦 TM 固体 泵浦功率
Rayleigh reciprocity relations: Applications
6
作者 林巨 李小雷 王宁 《中国物理B:英文版》 SCIE EI CAS CSCD 2016年第12期17-24,共8页
Classical reciprocity relations have wide applications in acoustics, from field representation to generalized optical theorem. In this paper we introduce our recent results on the applications and generalization of cl... Classical reciprocity relations have wide applications in acoustics, from field representation to generalized optical theorem. In this paper we introduce our recent results on the applications and generalization of classical Rayleigh reciprocity relation: higher derivative reciprocity relations as a generalization of the classical one and a theoretical proof on the Green’s function retrieval from volume noises. 展开更多
关键词 互惠关系 应用 瑞利 格林函数 衍生物 声学 定理 光学
MicroRNA-34a、Notch1在肝细胞肝癌组织中的表达及临床意义 预览 被引量:2
7
作者 于泳 李霄 +8 位作者 周亮 窦科峰 李小磊 阮柏 张卓超 戴斌 汪建林 杨西胜 王德盛 《世界华人消化杂志》 CAS 北大核心 2014年第14期1943-1952,共10页
目的:观察microRNA-34a、Notch1在肝细胞肝癌(hepatocellular carcinoma,HCC)组织的表达,探讨其与HCC发生、发展、临床病理特征的关系及可能的临床意义.方法:在132例HCC组织及癌旁组织、49例正常肝脏组织中应用实时荧光定量PCR法检... 目的:观察microRNA-34a、Notch1在肝细胞肝癌(hepatocellular carcinoma,HCC)组织的表达,探讨其与HCC发生、发展、临床病理特征的关系及可能的临床意义.方法:在132例HCC组织及癌旁组织、49例正常肝脏组织中应用实时荧光定量PCR法检测microRNA-34a的表达和免疫组织化学染色检测Notch 1表达.结果:HCC组织中microRNA-34a表达水平明显低于癌旁组织(2.57±0.71,P=0.003)及正常肝组织(8.65±0.21,P〈0.001),在转移性癌组织中microRNA-34a表达水平明显低于非转移性癌组织(1.34±0.13,P=0.014),差异均有统计学意义.Notch1的免疫组织化学染色结果:HCC组织中Notch1表达水平明显高于癌旁组织(3.12±0.67,P=0.001)及正常肝组织(4.65±0.14,P〈0.001),转移性癌组织高于非转移性癌组织(2.14±0.37,P=0.008),差异有统计学意义.MicroRNA-34a与Notch1在HCC及癌旁组织中均成负相关,相关系数分别为(r=-0.259,P=0.003)、(r=-0.274,P=0.002),两者在HCC组织中的表达与患者年龄、性别、肝功能、肿瘤部位、是否合并肝硬变、是否伴随肝炎病毒感染及甲胎蛋白(α-fetoprotein,αFP或AFP)含量无相关性(P〉0.05),而与肿瘤恶性程度、肿瘤个数、肿瘤体积、淋巴结转移、浸润深度、TNM分期密切相关(P〈0.05).Kaplan-Meier分析显示:microRNA-34a低表达、Notch1高表达组患者术后3年生存率(11.3%),明显低于microRNA-34a高表达、Notch1低表达组(34.7%),差异具有统计学意义(χ2=38.163,P=0.011).结论:MicroRNA-34a在HCC组织中较癌旁和正常肝脏组织明显减低,其通过逆向调节靶蛋白Notch1参与肿瘤肿瘤恶性行为;检测microRNA-34a及Notch1在HCC组织中的表达情况,对HCC的临床诊断、治疗及预后判断有潜在的重要意义. 展开更多
关键词 肝脏肿瘤 肝细胞肝癌 癌旁组织 MicroRNA-34a NOTCH1 生存分析 免疫组织化学
在线阅读 免费下载
白内障超声乳化吸除术后角膜内皮细胞变化的研究 预览 被引量:17
8
作者 刘婕 赵娴 +3 位作者 邵丽静 左建霞 李小磊 赵欣 《国际眼科杂志》 CAS 2014年第12期2247-2249,共3页
目的:观察不同类型白内障患者超声乳化吸除术后角膜内皮细胞的变化。 方法:随机选取我院老年性白内障、糖尿病性白内障及高度近视并发性白内障患者各30例30眼,均行白内障超声乳化吸除联合人工晶状体植入手术,于术前及术后1 wk采用非... 目的:观察不同类型白内障患者超声乳化吸除术后角膜内皮细胞的变化。 方法:随机选取我院老年性白内障、糖尿病性白内障及高度近视并发性白内障患者各30例30眼,均行白内障超声乳化吸除联合人工晶状体植入手术,于术前及术后1 wk采用非接触型角膜内皮显微镜行角膜内皮细胞密度及六角形细胞比例检查。 结果:三组术前角膜内皮细胞密度及六角形细胞比例比较,差异无统计学意义(P〉0.05)。三组术后1wk角膜内皮细胞密度分别为2496.86±298.96/mm2,2379.51±375.13/mm2,2425.38±312.68/mm2,六角形细胞比例分别为(46.20±12.03)%,(43.44±13.99)%,(44.35±8.13)%。三组术后1 wk角膜内皮细胞密度及六角形细胞比例均较术前减少,其差异均有统计学意义(P〈0.05)。术后各组间比较,糖尿病性白内障组和高度近视并发性白内障组术后均较老年性白内障组术后降低,其中糖尿病性白内障组术后角膜内皮细胞密度和六角形细胞比例降低较明显,与老年性白内障组比较,其差异有统计学意义(P〈0.05)。 结论:糖尿病性白内障及高度近视并发性白内障患者角膜内皮对超声乳化手术的耐受性降低,对角膜内皮细胞应行准确术前评估及术中保护。 展开更多
关键词 白内障 超声乳化手术 角膜内皮细胞 糖尿病 高度近视
在线阅读 下载PDF
Phase analysis and thermal conductivity of in situ O'-sialon/β-Si3N4 composites 预览 被引量:2
9
作者 Xiao-lei Li Xiao-Liang Chen Hui-ming Ji Xiao-hong Sun Ling-ge Zhao 《矿物冶金与材料学报:英文版》 SCIE EI CAS CSCD 2012年第8期757-761,共5页
典型 O-sialon-based 陶艺与西克斯·艾尔 x O1+x N2x 的一个公式,在 x 被放作为 0.25 的地方,被制作由在原处合成。Si3N4, Al2O3,和 SiO2 粉末被用作原料,并且 MgO 和 Y2O3 作为 sintering 添加剂被增加。所有样品是在在 0.25... 典型 O-sialon-based 陶艺与西克斯·艾尔 x O1+x N2x 的一个公式,在 x 被放作为 0.25 的地方,被制作由在原处合成。Si3N4, Al2O3,和 SiO2 粉末被用作原料,并且 MgO 和 Y2O3 作为 sintering 添加剂被增加。所有样品是在在 0.250.30 MPa 的一个氮压力下面的不同温度的 sintered,并且他们的微观结构,阶段内容,和热电导率被评估。热传导性上的 O-sialon 和 -Si3N4 的效果被数字计算详细分析。在类似的孔的情况中, O-sialon-based 陶艺的热传导性与 O-sialon/-Si3N4 增加的比率减少了。当比率是 12 时,在 1360t 的 O-sialon 陶艺 sintered 的热传导性 展开更多
关键词 赛隆陶瓷 原位合成 氮化硅 热传导率 物相分析 复合材料 二氧化硅粉末 数值计算分析
在线阅读 下载PDF
Experimental analysis of crack tip fields in rubber materials under large deformation 预览 被引量:3
10
作者 Xia Xiao Hai-Peng Song +3 位作者 Yi-Lan Kang Xiao-Lei Li Xiao-Hua Tan Hao-Yun Tan 《力学学报:英文版》 SCIE EI CAS CSCD 2012年第2期 432-437,共6页
一个 three-nested-deformation 模型被建议与大变丑在橡皮状的材料描述裂缝尖端地。模特儿被测量在里面飞机和 out-of-plane 变丑的分发鼓舞。我和混合模式(模式 III ) 折断的模式调节的在两个下面的裂缝尖端地的 inplane 排水量被使... 一个 three-nested-deformation 模型被建议与大变丑在橡皮状的材料描述裂缝尖端地。模特儿被测量在里面飞机和 out-of-plane 变丑的分发鼓舞。我和混合模式(模式 III ) 折断的模式调节的在两个下面的裂缝尖端地的 inplane 排水量被使用数字 Moir 测量 ?? P 展开更多
关键词 裂纹尖端场 实验分析 橡胶材料 变形条件 数字散斑相关方法 平面变形 位移测量 橡胶类材料
在线阅读 下载PDF
Adaptive online prediction method based on LS-SVR and its application in an electronic system
11
作者 Yang-ming GUO Cong-bao RAN Xiao-lei LI Jie-zhong MA 《浙江大学学报:C卷英文版》 SCIE EI 2012年第12期881-890,共10页
Health trend prediction has become an effective way to ensure the safe operation of highly reliable systems,and online prediction is always necessary in many real applications.To simultaneously obtain better or accept... Health trend prediction has become an effective way to ensure the safe operation of highly reliable systems,and online prediction is always necessary in many real applications.To simultaneously obtain better or acceptable online prediction accuracy and shorter computing time,we propose a new adaptive online method based on least squares support vector regression(LS-SVR).This method adopts two approaches.One approach is that we delete certain support vectors by judging the linear correlation among the samples to increase the sparseness of the prediction model.This approach can control the loss of useful information in sample data,improve the generalization capability of the prediction model,and reduce the prediction time.The other approach is that we reduce the number of traditional LS-SVR parameters and establish a modified simple prediction model.This approach can reduce the calculation time in the process of adaptive online training.Simulation and a certain electric system application indicate preliminarily that the proposed method is an effective prediction approach for its good prediction accuracy and low computing time. 展开更多
关键词 在线预测 电子系统 自适应 应用 支持向量回归机 计算时间 预测模型 预测精度
肠上皮细胞来源整合素αVβ6对树突状细胞功能的影响 预览
12
作者 李晓蕾 郑鹏远 +1 位作者 李付广 刘志强 《世界华人消化杂志》 CAS 北大核心 2012年第17期 1553-1558,共6页
目的:研究肠上皮细胞(intestinal epithelial cells,IEC)来源的整合素αVβ6对骨髓来源树突状细胞(bone marrow-derived dendritic cells,BMDCs)功能的影响.方法:分离、培养小鼠IEC和DC,IEC经卵清蛋白(ovalbumin,OVA)刺激后,多... 目的:研究肠上皮细胞(intestinal epithelial cells,IEC)来源的整合素αVβ6对骨髓来源树突状细胞(bone marrow-derived dendritic cells,BMDCs)功能的影响.方法:分离、培养小鼠IEC和DC,IEC经卵清蛋白(ovalbumin,OVA)刺激后,多步离心法制备外泌体(exosome).应用免疫胶体金对外泌体中的整合素αVβ6进行定性,通过免疫磁珠技术,分离获取DC,流式细胞仪检测分离后DC纯度.然后将分离后的DC分5组,分别为空白组、OVA组、外泌体组、外泌体+抗整合素αVβ6抗体组、外泌体+羊抗小鼠IgG组,观察各组对脂多糖(lipopolysaccharide,LPS)刺激的反应.ELISA法检测DC在LPS刺激前后IL-12p70的表达.LPS刺激前活性转化生长因子β1(transforming growth factor-β1,TGF-β1)以及总TGF-β1的表达.结果:OVA组与空白组相比,总TGF-β1表达明显增高(226.636±40.355vs176.947±23.072,P〈0.05),但活化TGF-β1表达无明显变化(P〉0.05).空白组与外泌体+抗整合素αVβ6抗体组相比,活化TGF-β1表达无明显变化(P〉0.05),但总TGF-β1表达明显增高(P〈0.05).外泌体组和外泌体+羊抗小鼠IgG组与空白组相比,活化TGF-β1和总TGF-β1表达均明显增高(P〈0.05).相比空白对照组,LPS刺激48h后外泌体组和外泌体+羊抗小鼠IgG组IL-12p70表达明显降低(P〈0.05),OVA组及外泌体+抗整合素αVβ6抗体组IL-12p70表达无明显差异(P〉0.05).结论:IEC来源整合素αVβ6能增加DC活性,以及TGF-β1和总TGF-β1的表达量,并拮抗LPS对BMDC的促成熟作用. 展开更多
关键词 外泌体 整合素ΑVΒ6 树突状细胞 转化生长因子Β1
在线阅读 免费下载
川芎嗪对肝创伤后早期大鼠残肝组织保护作用的评价 预览 被引量:2
13
作者 陈战 窦科峰 +5 位作者 王德盛 杨平 蔡佳辰 李霄 刘志佳 李小磊 《世界华人消化杂志》 CAS 北大核心 2011年第32期 3291-3296,共6页
目的:研究川芎嗪干预对68%(2/3)肝切除大鼠术后残肝组织的保护作用,以及其对肝脏核因子κB(NF-κB)激活的影响.方法:60只SD大鼠随机分成3组(每组20只),均行68%(2/3)肝切除术.术后30 min分别给予以下处理:A组腹腔注射生理盐... 目的:研究川芎嗪干预对68%(2/3)肝切除大鼠术后残肝组织的保护作用,以及其对肝脏核因子κB(NF-κB)激活的影响.方法:60只SD大鼠随机分成3组(每组20只),均行68%(2/3)肝切除术.术后30 min分别给予以下处理:A组腹腔注射生理盐水,B组注射吡咯烷二硫代氨甲基甲酸盐(PDTC),C组注射川芎嗪.术后2 h、6 h、10 h和24 h观察各组大鼠术后一般情况和血清转氨酶变化.组织切片观察残肝组织病理状况,Western-blot方法检测NF-κB的激活.结果:C组大鼠残肝组织切片细胞水肿较A组轻:丙氨酸氨基转氨酶(ALT)在6 h(488.9 U/L±59.2 U/L)、10 h(670.0 U/L±73.4 U/L),较A组(651.6 U/L±65_3 U/L,930.0 U/L±62.9 U/L)低,P〈0.05;天门冬氨酸氨基转移酶(AST)在6 h(11113.1 U/L±138.7 U/L)、10 h(1388.2 U/L±209.6 U/L),较A组(1315.0 U/L±111.0 U/L,1728.4 U/L±87_3 U/L)低,P〈0.05:NF-κB的激活在术后2 h(0.78±0.04)和10 h(0.75±0.07)均低于A组(均为1),高于B组(0.68±0.09,0.66±0.04),P〈0.05,6 h(0.71±0.07)低于A组(1),P〈0.05,与B组(0.64±0.09)接近,P〉0.05.结论:川芎嗪通过抑制NF-κB的激活,对创伤后肝组织产生保护作用,其保护作用接近于PDTC. 展开更多
关键词 川芎嗪 肝创伤 核因子ΚB
在线阅读 免费下载
siRNA沉默Bmi-1基因对人肝癌细胞株MHCC97-H侵袭迁移能力的影响 预览 被引量:4
14
作者 李小磊 宋文杰 +5 位作者 陈战 陈亚峰 李霄 周亮 张福琴 窦科峰 《世界华人消化杂志》 CAS 北大核心 2011年第36期 3643-3648,共6页
目的:探讨RNA干扰技术沉默Bmi-1基因表达后,对人肝癌细胞株MHCC97-H侵袭迁移能力的影响.方法:设计并合成针对Bmi-1基因序列特异性的双链小干扰RNA(Bmi-1-siRNA),转染高转移性人肝癌细胞株MHCC97-H,用流式细胞仪观察转染效率,荧光实... 目的:探讨RNA干扰技术沉默Bmi-1基因表达后,对人肝癌细胞株MHCC97-H侵袭迁移能力的影响.方法:设计并合成针对Bmi-1基因序列特异性的双链小干扰RNA(Bmi-1-siRNA),转染高转移性人肝癌细胞株MHCC97-H,用流式细胞仪观察转染效率,荧光实时定量PCR和Westernblot检测Bmi-1基因的mRNA和蛋白表达水平;通过体外Transwell小室基质侵袭和迁移实验,观察Bmi-1表达沉默后对人肝癌细胞株MHCC97-H侵袭和迁移能力的影响.结果:将针对Bmi-1基因序列特异性的小干扰RNA(Bmi-1-siRNA)转染高转移性人肝癌细胞株MHCC97-H后,流式细胞仪显示,转染效率可达到91%.与空白组、对照siRNA组相比,实验组Bmi-1-siRNA能有效抑制MHCC97-H细胞中Bmi-1基因的mRNA(F=56.199,P〈0.05)和蛋白表达水平.通过Transwell小室基质侵袭和迁移实验,我们分析了不同组细胞的侵袭迁移能力.结果发现,与空白组、对照siRNA组相比,Bmi-1-siRNA转染的MHCC97-H细胞穿透能力明显降低(F=186.66,12.746,P〈0.05).结论:沉默Bmi-1基因表达可抑制肝癌细胞株MHCC97-H的侵袭迁移力. 展开更多
关键词 RNA干扰 肝肿瘤 BMI-1 侵袭 转移
在线阅读 免费下载
Aroma Volatile Compound Analysis of SPME Headspace and Extract Samples from Crabapple (Malus sp.) Fruit Using GC-MS 预览 被引量:1
15
作者 LI Xiao-lei KANG Lua HU Jing-jing LI Xue-fei SHEN Xiang 《中国农业科学:英文版》 CAS CSCD 2008年第12期 1451-1457,共7页
Volatile compounds from the ripened crabapple fruit of six varieties (Red Splendor, Strawberry Parfait, Pink Spire, Radiant, Sparkler, and Flame) were analyzed by the use of the SPME/GC/MS method. The changes in the v... Volatile compounds from the ripened crabapple fruit of six varieties (Red Splendor, Strawberry Parfait, Pink Spire, Radiant, Sparkler, and Flame) were analyzed by the use of the SPME/GC/MS method. The changes in the volatiles between the ripened and upon full maturity fruit states were studied in Red Splendor and Strawberry Parfait. An effort was made to summarize an effective method for searching and identifying new idioplasms containing a particular fruit aroma within Malus. A total of 37 compounds were identified from the sample. The main aroma volatiles of the six varieties of fruit were comprised of 2-hexenal, 3-hexenal, hexanal, 2,4-hexadienal, benzaldehyde, diethyl phthalate. The main volatile compound of the crabapple fruit was 2-hexenal, but the relative content percentages were different (45.37, 21.98, 33.56, 32.21, 38.60, and 45.88%). The aroma components accumulated differently as the fruits ripened. The relative content of aldehydes and esters decreased as alcohols increased after the Red Splendor and Strawberry Parfait fruit ripened. For Red Splendor, the main volatile was still 2-hexenal, but the relative content decreased to 42.89%, and the relative content of alcohols increased by 13.86% as aldehydes and esters declined by 12.16 and 7.18%, respectively. For Strawberry Parfait, the main volatile was changed to cyclohexanol, and the relative content increased to 46.43%, while the relative content of alcohols increased by 49.03% as aldehydes and esters declined by 23.74 and 9.34%, respectively. 展开更多
关键词 水果 草莓 香气 化合物
在线阅读 下载PDF
A Sound Field Separation and Reconstruction Technique Based on Reciprocity Theorem and Fourier Transform
16
作者 李小雷 王宁 +1 位作者 高大治 李琪 《中国物理快报:英文版》 SCIE CAS CSCD 2018年第11期42-45,共4页
关键词 FOURIER 重建技术 定理 分离 高分辨率 波浪能 信号 测量
关节镜下两种固定方式治疗前交叉韧带胫骨止点撕脱骨折的临床研究 预览 被引量:5
17
作者 李小磊 魏鹏 邱忠鹏 《中国内镜杂志》 2018年第2期38-42,共5页
目的 探讨关节镜下螺钉与缝线固定治疗前交叉韧带(ACL)胫骨止点撕脱骨折临床疗效差异。方法 研究对象选取ACL胫骨止点撕脱骨折患者共80例,以随机数字表法分为A组(40例)和B组(40例),分别在关节镜下行缝线与螺钉固定治疗;比较两组... 目的 探讨关节镜下螺钉与缝线固定治疗前交叉韧带(ACL)胫骨止点撕脱骨折临床疗效差异。方法 研究对象选取ACL胫骨止点撕脱骨折患者共80例,以随机数字表法分为A组(40例)和B组(40例),分别在关节镜下行缝线与螺钉固定治疗;比较两组患者手术时间、随访Lysholm膝关节评分、国际膝关节评分委员会(IKDC)膝关节功能主观量表评分、患健侧位移差值及屈曲挛缩发生率。结果 B组患者手术时间明显短于A组(P〈0.05);两组患者随访Lysholm评分、IKDC评分及患健侧位移差值比较差异无统计学意义(P〉0.05);两组患者随访屈曲挛缩发生率比较差异无统计学意义(P〉0.05)。结论 关节镜下螺钉与缝线固定治疗ACL胫骨止点撕脱骨折在提高复位稳定性和改善关节活动功能方面效果接近,但关节镜下螺钉固定方案应用可有效缩短手术时间,降低操作难度。 展开更多
关键词 关节镜 螺钉 缝线 前交叉韧带胫骨止点撕脱骨折 疗效
在线阅读 下载PDF
单分子磁体研究的最新进展
18
作者 朱振华 郭梅 +1 位作者 李晓磊 唐金魁 《中国科学:化学》 CSCD 北大核心 2018年第8期790-803,共14页
宏观上表现出可测的慢磁弛豫行为的单个分子称为单分子磁体,它主要包括过渡金属单分子磁体和稀土单分子磁体,其在量子计算、高密度信息存储以及自旋器件等方面的潜在应用吸引了研究者的广泛关注.本文介绍了单分子磁体研究的最新进展,重... 宏观上表现出可测的慢磁弛豫行为的单个分子称为单分子磁体,它主要包括过渡金属单分子磁体和稀土单分子磁体,其在量子计算、高密度信息存储以及自旋器件等方面的潜在应用吸引了研究者的广泛关注.本文介绍了单分子磁体研究的最新进展,重点总结了构筑高性能单分子磁体的合成策略. 展开更多
关键词 单分子磁体 过渡金属 稀土离子 弛豫
Right cryptorchid testicular metastasis from descending colon carcinoma: a case report of "Krukenberg tumor" in male
19
作者 Qi Xu Xiao-Lei Li +3 位作者 Ya-Wei Yu Lai-Rong Dong Jian-Guo Shen Lin-Bo Wang 《亚洲男性学杂志:英文版》 SCIE CAS CSCD 2015年第1期171-172,共2页
关键词 病例报告 男性 结肠癌 卵巢肿瘤 睾丸 隐睾 转移性 细胞内
浓缩苹果汁加工过程挥发性风味物质变化规律 预览 被引量:2
20
作者 郑宇 刘畅 +4 位作者 李林洁 白晓磊 李淑静 郑文杰 王敏 《食品与发酵工业》 CAS CSCD 北大核心 2015年第11期121-128,共8页
采用顶空固相微萃取结合气相色谱-质谱技术对浓缩苹果汁加工过程挥发性风味物质组成及其变化规律进行了分析.浓缩苹果汁加工过程中检测到主要挥发性风味物质79种,其中酯类化合物种类最多,共33种,其次为醛类10种,醇类7种.酶解、巴氏杀菌... 采用顶空固相微萃取结合气相色谱-质谱技术对浓缩苹果汁加工过程挥发性风味物质组成及其变化规律进行了分析.浓缩苹果汁加工过程中检测到主要挥发性风味物质79种,其中酯类化合物种类最多,共33种,其次为醛类10种,醇类7种.酶解、巴氏杀菌、超滤和浓缩等工序对物料的挥发性风味物质影响较大,其中果胶酶解后醇类和酯类化合物相对浓度分别增加了121.90%和42.29%,醛类化合物相对浓度降低了97.07%;前巴氏杀菌后醇类和酯类化合物相对浓度分别下降了45.47%和3.41%,醛类化合物相对浓度增加了97.94%;超滤后醇类、酯类化合物相对浓度分别下降了57.86%和41.76%;蒸发浓缩后醇类、酯类和醛类相对浓度分别下降了99.94%、85.83%和94.34%. 展开更多
关键词 浓缩苹果汁 挥发性风味物质 酶解 巴氏杀菌 超滤
在线阅读 免费下载
上一页 1 2 5 下一页 到第
使用帮助 返回顶部 意见反馈