期刊文献+
共找到18,319篇文章
< 1 2 250 >
每页显示 20 50 100
甲状腺纤维化的中医治疗思路 预览
1
作者 柏力萄 赵静 +3 位作者 李俊 吴瑞 魏璠 魏军平 《世界科学技术:中医药现代化》 CSCD 北大核心 2019年第2期267-271,共5页
甲状腺纤维化是各种因素刺激下,出现的纤维性改变,是多种甲状腺疾病共有的病理过程。甲状腺纤维化严重影响患者生存质量,故针对纤维化治疗具有重要意。中药辨证治疗甲状腺纤维化以疏肝行气、健脾补肾、活血化瘀、祛痰除湿、软坚散结为... 甲状腺纤维化是各种因素刺激下,出现的纤维性改变,是多种甲状腺疾病共有的病理过程。甲状腺纤维化严重影响患者生存质量,故针对纤维化治疗具有重要意。中药辨证治疗甲状腺纤维化以疏肝行气、健脾补肾、活血化瘀、祛痰除湿、软坚散结为总原则。根据纤维化早、中、晚三期分别采用半夏厚朴汤,补中益气汤,桂附地黄汤合真武汤加丹参、地龙、贝母等方药治疗。并加入穿山龙、夏枯草、雷公藤、山慈菇、黄药子、人参皂甙等具有抗甲状腺纤维化的中药及单体,以期为临床治疗提供一种新的思路。 展开更多
关键词 甲状腺 纤维化 中医药治疗 抗纤维化
在线阅读 下载PDF
Current therapies and novel approaches for biliary diseases 预览
2
作者 Indu G Rajapaksha Peter W Angus Chandana B Herath 《世界胃肠病理生理学杂志:英文版(电子版)》 2019年第1期1-10,共10页
Chronic liver diseases that inevitably lead to hepatic fibrosis, cirrhosis and/or hepatocellular carcinoma have become a major cause of illness and death worldwide. Among them, cholangiopathies or cholestatic liver di... Chronic liver diseases that inevitably lead to hepatic fibrosis, cirrhosis and/or hepatocellular carcinoma have become a major cause of illness and death worldwide. Among them, cholangiopathies or cholestatic liver diseases comprise a large group of conditions in which injury is primarily focused on the biliary system. These include congenital diseases (such as biliary atresia and cystic fibrosis), acquired diseases (such as primary sclerosing cholangitis and primary biliary cirrhosis), and those that arise from secondary damage to the biliary tree from obstruction, cholangitis or ischaemia. These conditions are associated with a specific pattern of chronic liver injury centered on damaged bile ducts that drive the development of peribiliary fibrosis and, ultimately, biliary cirrhosis and liver failure. For most, there is no established medical therapy and, hence, these diseases remain one of the most important indications for liver transplantation. As a result, there is a major need to develop new therapies that can prevent the development of chronic biliary injury and fibrosis. This mini-review briefly discusses the pathophysiology of liver fibrosis and its progression to cirrhosis. We make a special emphasis on biliary fibrosis and current therapeutic options, such as angiotensin converting enzyme-2 (known as ACE2) over-expression in the diseased liver as a novel potential therapy to treat this condition. 展开更多
关键词 Chronic liver disease BILIARY FIBROSIS CURRENT THERAPIES for BILIARY FIBROSIS ANGIOTENSIN CONVERTING enzyme-2 Gene therapy
在线阅读 免费下载
Novel non-invasive score to predict cirrhosis in the era of hepatitis C elimination:A population study of ex-substance users in Singapore 预览
3
作者 Yue Zhao Prem Harichander Thurairajah +3 位作者 Rahul Kumar Jessica Tan Eng Kiong Teo John Chen Hsiang 《国际肝胆胰疾病杂志:英文版》 SCIE CAS CSCD 2019年第2期143-148,共6页
Background:Chronic hepatitis C infection is common among people with history of substance use.Liver fibrosis assessment is a barrier to linkage to care,particularly among those with history of substance users.The use ... Background:Chronic hepatitis C infection is common among people with history of substance use.Liver fibrosis assessment is a barrier to linkage to care,particularly among those with history of substance users.The use of non-invasive scores can be helpful in predicting liver cirrhosis in the era of HCV elimination,especially in countries where transient elastography(TE)is not available.We compared the commonly used non-invasive scores with a novel non-invasive score in predicting liver cirrhosis in this population.Methods:HCV patients with history of substance use between 2011 and 2016 were analyzed.All patients had TE for liver fibrosis assessment.Clinical performance of established non-invasive scores for fibrosis assessment and novel score were compared.Youden’s index was used to determine optimal cut-off of the novel score.Results:A total of 579 patients were included.In multivariate logistic regression,cirrhosis on TE was associated with age(P=0.002),aspartate aminotransferase(AST)(P=0.004),and platelet count(P<0.001),but not alanine aminotransferase(ALT)(P=0.896).These form the components of modified AST-toplatelet ratio index(APRI)score.Modified APRI was superior to APRI in predicting cirrhosis(AUROC,0.796 vs.0.770,P=0.007),but not fibrosis-4 score(FIB-4)(P=1.00).Modified APRI at cut-off of 4 has sensitivity,specificity and negative predictive value(NPV)of 94.4%,26.9%and 92.6%,respectively,and at 19,has sensitivity,specificity and positive predictive value(PPV)of 33.3%,96.2%and 77.1%,respectively.FIB-4 has a NPV and PPV of 88.6%,41.8%and 78.5%,77.6%,at cut-off of 1.45 and 3.25,respectively.Using the cut-off of 4 and 14 for modified APRI,32.5%of patients can be correctly classified and misses out only 5.6%of cirrhosis patients.Conclusions:Modified APRI score is superior in predicting cirrhosis in HCV population,with 32.5%of the population being correctly classified using cut-off of 4 and 14.Further studies are required to validate the findings. 展开更多
关键词 Hepatitis C Cirrhosis Fibrosis assessment AST-to-platelet ratio index Modified APRI Fibrosis-4 SCORE
在线阅读 下载PDF
c.753_754delAG,a novel CFTR mutation found in a Chinese patient with cystic fibrosis:A case report and review of the literature 预览
4
作者 Yu-Qing Wang Chuang-Li Hao +4 位作者 Wu-Jun Jiang Yan-Hong Lu Hui-Quan Sun Chun-Yan Gao Min Wu 《世界临床病例杂志》 2019年第15期2110-2119,共10页
BACKGROUND Cystic fibrosis(CF)is rare in Asian populations relative to the Caucasian population.In this paper,we report the cystic fibrosis transmembrane conductance regulator(CFTR)variation in a family of Chinese CF ... BACKGROUND Cystic fibrosis(CF)is rare in Asian populations relative to the Caucasian population.In this paper,we report the cystic fibrosis transmembrane conductance regulator(CFTR)variation in a family of Chinese CF patients,and systematically review the previous literature.CASE SUMMARY Here we report a 30-month-old Chinese girl who was diagnosed with CF based on her history and symptoms such as recurrent productive cough,wheezing with repeated infection of Pseudomonas aeruginosa,and parasinusitis.Chest computed tomography(CT)scanning revealed obvious exudative lesions and bilateral bronchiectasis.Liver CT scanning revealed a low-density lesion in the left lobe of the liver.A diagnosis of CF was made based upon CFTR gene tests.The CFTR gene was sequenced using the blood samples of her and her parents and showed a heterozygous novel missense mutation of c.753_754delAG in exon 7.In addition,a heterozygous c.1240 C>T mutation was found in exon 10 of the CFTR.The mutation c.753_754delAG was verified to have been inherited from her mother,and the c.1240 C>T mutation was from her father who was diagnosed with congenital absence of vas deferens.CONCLUSION A novel mutation of CFTR,c.753_754delAG,was found in a Chinese CF child.c.2909G>A is the most common mutation among Chinese CF patients. 展开更多
关键词 CYSTIC FIBROSIS CYSTIC FIBROSIS TRANSMEMBRANE conductance regulator MUTATION CHINESE children Case report
在线阅读 免费下载
Anluohuaxianwan Alleviates Carbon Tetrachloride-Induced Hepatic Fibrosis in Rats through Upregulation of Peroxisome Proliferator-Activated Receptor-Gamma and Downregulation of Nuclear Factor-Kappa B/IκBα Signaling Pathway
5
作者 Lin Wang Wei Lu +4 位作者 Yu-Hua Gao Hai-Jiang Yan Fei Pei Xue-En Liu Hui Zhuang 《世界中医药杂志:英文版》 2019年第2期95-103,共9页
Objective:The aim of this study was to investigate the effects of traditional Chinese medicine Anluohuaxianwan(ALHXW)on peroxisome proliferator-activated receptor-gamma(PPARγ)and nuclear factor-kappa B(NF-κB)signali... Objective:The aim of this study was to investigate the effects of traditional Chinese medicine Anluohuaxianwan(ALHXW)on peroxisome proliferator-activated receptor-gamma(PPARγ)and nuclear factor-kappa B(NF-κB)signaling pathways using a rat model of carbon model groups were gavaged with saline for 6 weeks.Liver function was measured,and liver fibrosis and necroinflammation were assessed.Protein and messenger RNA expression levels of PPARγ,NF-κB,and Inhibitorαof NF-κB(IκBα)were analyzed by Western blot and reverse transcription–quantitative polymerase chain reaction.Results:ALHXW markedly alleviated liver injury compared with the model group,as indicated by the improvements in disease status,the morphology of liver and spleen,the liver and spleen indexes,and liver function.The extent of liver fibrosis was improved,hepatic stellate cell activation was inhibited,the expression of PPARγand IκBαwas significantly higher,and the expression of NF-κB was significantly lower in the treatment group as compared with the model group.Conclusions:ALHXW treatment can alleviate CCl4-induced liver fibrosis in rats, and the potential antifibrogenic mechanisms may occur through the upregulation of PPARγ expression and downregulation of NF-κB/IκBα signaling pathway. 展开更多
关键词 Anluohuaxianwan hepatic fibrosis mechanism nuclear factor-kappa B/IκBα PEROXISOME proliferator-activated receptor-gamma
硫化氢对甲基胍加重残余肾功能损害的保护作用
6
作者 邓薇 王利民 +2 位作者 谢红武 黄远莲 邓荣芳 《解剖学研究》 CAS 2019年第3期192-196,共5页
目的检测残余肾功能相关指标,明确硫化氢(H2S)对甲基胍加重肾功能损害的保护作用。方法大鼠随机分为假手术(SH)组、和5/6肾切除肾衰模型(MD)组、甲基胍(MH)组及甲基胍+硫化氢(MH+H2S)组,检测各组血清尿素氮、肌酐,观察肾脏组织的病理改... 目的检测残余肾功能相关指标,明确硫化氢(H2S)对甲基胍加重肾功能损害的保护作用。方法大鼠随机分为假手术(SH)组、和5/6肾切除肾衰模型(MD)组、甲基胍(MH)组及甲基胍+硫化氢(MH+H2S)组,检测各组血清尿素氮、肌酐,观察肾脏组织的病理改变,免疫组化法检测肾组织内TGF、CTGF、FN的表达水平,TUNEL观察细胞的凋亡,Western blot检测自噬相关蛋白Atg3和Beclin的表达水平。结果 MD组、MH组及MH+H2S组较假手术组血清尿素氮、肌酐显著升高(P<0.05),肾小球体积增大、系膜细胞及基质明显增生,免疫组化染色显示TGF、CTGF、FN阳性面积表达显著增强(P<0.05),细胞凋亡率显著增加(P<0.05),Western blot结果显示,Atg3和Beclin的表达水平显著增强(P<0.05)。加入甲基胍后血清尿素氮、肌酐较模型组明显升高(P<0.05),肾小球体积较模型组明显增大、系膜细胞及基质较模型组增生严重,免疫组化染色显示TGF、CTGF、FN均较模型组表达增强(P<0.05),细胞凋亡率较模型组显著增加(P<0.05),Atg3和Beclin的表达水平显著增强(P<0.05)。而加入H2S后血清尿素氮、肌酐明显下降(P<0.05),免疫组化显示TGF、CTGT、FN表达减弱(P<0.05),细胞凋亡率显著降低(P<0.05),Beclin的表达水平进一步增强(P<0.05)。结论甲基胍可能是残余肾功能进一步下降的关键因素,H2S可能通过自噬激活逆转甲基胍的肾毒性作用。 展开更多
关键词 慢性肾功能衰竭 甲基胍 硫化氢 纤维化 自噬
Roles of hepatic stellate cells in acute liver failure:From the perspective of inflammation and fibrosis 预览
7
作者 Juan Li Ying-Ren Zhao Zhen Tian 《世界肝病学杂志:英文版(电子版)》 2019年第5期412-420,共9页
Acute liver failure(ALF)usually results in hepatocellular dysfunction and coagulopathy and carries a high mortality rate.Hepatic stellate cells(HSCs)are famous for their role in liver fibrosis.Although some recent stu... Acute liver failure(ALF)usually results in hepatocellular dysfunction and coagulopathy and carries a high mortality rate.Hepatic stellate cells(HSCs)are famous for their role in liver fibrosis.Although some recent studies revealed that HSCs might participate in the pathogenesis of ALF,the accurate mechanism is still not fully understood.This review focuses on the recent advances in understanding the functions of HSCs in ALF and revealed both protective and promotive roles during the pathogenesis of ALF:HSC activation participates in the maintenance of cell attachment and the architecture of liver tissue via extracellular matrix production and assists liver regeneration by producing growth factors;and HSC inflammation plays a role in relaying inflammation signaling from sinusoids to parenchyma via secretion of inflammatory cytokines.A better understanding of roles of HSCs in the pathogenesis of ALF may lead to improvements and novel strategies for treating ALF patients. 展开更多
关键词 ACUTE liver FAILURE HEPATIC stellate cells INFLAMMATION FIBROSIS
在线阅读 免费下载
NLRP3炎性体在心肌病和心律失常中的作用及研究进展
8
作者 李威 刘明鑫 唐艳红 《心脏杂志》 CAS 2019年第3期334-338,共5页
NLRP3炎性体是先天免疫反应的参与者,通过相关激活信号来触发炎症。NLRP3炎性体在心肌细胞和心脏成纤维细胞中表达,通过水解含半胱氨酸的天冬氨酸蛋白水解酶(caspase-1)前体生成caspase-1促使白介素(IL)-1β、IL-18的成熟和释放而导致... NLRP3炎性体是先天免疫反应的参与者,通过相关激活信号来触发炎症。NLRP3炎性体在心肌细胞和心脏成纤维细胞中表达,通过水解含半胱氨酸的天冬氨酸蛋白水解酶(caspase-1)前体生成caspase-1促使白介素(IL)-1β、IL-18的成熟和释放而导致细胞焦亡,其浸润影响到心肌病和心律失常等心血管疾病的发生发展过程。在这篇综述中,我们介绍了NLRP3炎性体在心肌病和心律失常中的作用及相关机制,对探索针对NLRP3炎症体在此类疾病中的治疗方案有重要意义。 展开更多
关键词 NLRP3炎性体 心肌病 心律失常 焦亡 纤维化
维生素D缺乏与酒精性肝病的相关性研究 预览
9
作者 杨冰冰 胡纯秋 +2 位作者 胡永迪 储兰兰 徐德祥 《安徽医科大学学报》 CAS 北大核心 2019年第8期1273-1276,共4页
目的 探讨维生素D缺乏与酒精性肝病(ALD)的相关性。方法 按照入选标准收集ALD患者70例,选取同期性别、年龄匹配的健康对照70例。收集完整的临床及生化检查资料,采用两独立样本t检验、秩和检验、Gamma检验、Logistic回归分析血清25-羟维... 目的 探讨维生素D缺乏与酒精性肝病(ALD)的相关性。方法 按照入选标准收集ALD患者70例,选取同期性别、年龄匹配的健康对照70例。收集完整的临床及生化检查资料,采用两独立样本t检验、秩和检验、Gamma检验、Logistic回归分析血清25-羟维生素D[25(OH)D]水平与ALD的相关性。结果 ALD组体质量指数、总胆红素、直接胆红素、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、谷氨酰转肽酶、碱性磷酸酶、空腹血糖等均高于对照组,差异均有统计学意义(P<0.05);而白细胞、血红蛋白、血小板、血清白蛋白、25(OH)D、血钙水平均低于对照组,差异均有统计学意义(P<0.05)。整体样本中,维生素D缺乏的群体发生ALD例数明显升高(Z=-2.135,P<0.05)。将ALD组分为酒精性脂肪肝组、酒精性肝炎组和酒精性肝硬化组,在维生素D缺乏和不足的群体中发生酒精性肝炎和酒精性肝硬化的例数明显增加(Gamma=-0.495,P<0.01)。多元Logistic回归分析在调整了年龄、空腹血糖、体质量指数等混杂因素后显示,25(OH)D水平是ALD发病的危险因素,25(OH)D水平与ALD呈负相关(OR=4.144,95%CI:1.191~14.415,P=0.025)。结论 维生素D缺乏可能是ALD炎症和纤维化进展的危险因素。 展开更多
关键词 维生素D 酒精性肝病 炎症 纤维化 危险因素
在线阅读 下载PDF
A pharmacological approach to study the active compounds in Jinshui Huanxian formula in treatment of pulmonary fibrosis
10
作者 Bai Yunping Zhao Peng +1 位作者 Wu Mingming Li Jiansheng 《中医杂志:英文版》 SCIE CAS CSCD 2019年第3期364-379,共16页
OBJECTIVE:To study the active compounds in Jinshui Huanxian formula in the treatment of pulmonary fibrosis with a pharmacological approach.METHODS:A systems pharmacology model,incorporating active compounds and target... OBJECTIVE:To study the active compounds in Jinshui Huanxian formula in the treatment of pulmonary fibrosis with a pharmacological approach.METHODS:A systems pharmacology model,incorporating active compounds and targets prediction,and herbal-comPound-target-disease network analysis,was established to predict the active substances and therapeutic mechanisms of Jinshui Huanxian formula.All compounds from the herbs of Jinshui Huanxian formula were obtained from drug database and the literature.Then,we analyzed the potential of herbs by predicting oral bioavailability and drug-likeness index.The compounds with oral bioavailability≥ 30% and drug-likeness index ≥ 0.18 were obtained as candidate compounds for further analysis.We then used the systematic drug targeting tool to screen the targets for candidate compounds.The potential targets were projected into Therapeutic Target Database to collect their corresponding diseases.The candidate compounds,potential targets and their related diseases were applied to construct the compound-target and target-disease network.RESULTS:Totally 136 compounds from Jinshui Huanxian formula and 265 potential targets were found.Compound-target network analysis suggested that different herbal drugs contained in the Jinshui Huanxian formula could regulate these similar targets to probably exert synergistic effect.Moreover,target proteins were mainly related to oxidoreductase activity,nicotinamide adenine dinucleotide phosphate binding,and G-protein coupled amine receptor activity,which might be associated with the therapeutic mechanisms of Jinshui Huanxian formula.In addition,Jinshui Huanxian formula was probably efficient for many different diseases,such as respiratory tract diseases,neoplasm,nervous system diseases,and cardiovascular diseases,according to target-disease network.CONCLUSION:This study may provide a method to explore the potential active compounds in Jinshui Huanxian formula used to treat pulmonary fibrosis. 展开更多
关键词 Pulmonary FIBROSIS PHARMACOLOGY Jinshui Huanxian FORMULA
microRNA在心脏重塑中的研究进展 预览
11
作者 陈阳 王婧 马丽杰 《基础医学与临床》 CSCD 2019年第4期589-593,共5页
microRNAs(miRNAs)通过对其靶基因的转录后调控参与胚胎发育、细胞增殖、分化及凋亡等过程,与心血管疾病的发生发展密切相关。心脏重塑目前缺乏有效的治疗靶点和有效的诊疗标志物,并且心脏重塑的机制研究还不够深入。目前发现miRNAs可... microRNAs(miRNAs)通过对其靶基因的转录后调控参与胚胎发育、细胞增殖、分化及凋亡等过程,与心血管疾病的发生发展密切相关。心脏重塑目前缺乏有效的治疗靶点和有效的诊疗标志物,并且心脏重塑的机制研究还不够深入。目前发现miRNAs可通过不同的分子机制调节心肌纤维化、心肌肥大以及能量代谢调节重塑。 展开更多
关键词 MICRORNAS 心脏重塑 纤维化 肥大
在线阅读 下载PDF
纤维化因子及NADPH氧化酶在心理应激诱导小鼠的食管纤维化中的表达及意义
12
作者 买买提·依斯热依力 吾布力卡斯木·吾拉木 +2 位作者 艾克拜尔·艾力 李义亮 克力木·阿不都热依木 《中国医师杂志》 CAS 2019年第5期672-676,共5页
目的研究胶原蛋白1(colla-1)、转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)及还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX-4)在慢性束缚应激(CRS)模型小鼠食管中的表达水平,探讨心理应激诱导食管纤维化的发生及氧化应激所产... 目的研究胶原蛋白1(colla-1)、转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)及还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX-4)在慢性束缚应激(CRS)模型小鼠食管中的表达水平,探讨心理应激诱导食管纤维化的发生及氧化应激所产生的影响。方法 20只雄性SPF级昆明小鼠随机分2组,即CRS组和对照组。CRS组小鼠每天在自制式束缚器中限制活动2 h,其余时间两组小鼠在相同环境中自由饮水摄食,实验持续14 d。通过Masson染色在电子显微镜下观察食管组织纤维化改变。采用免疫组化、实时定量RT-PCR、ELISA法检测NOX-4在小鼠食管中的表达,并通过实时定量RT-PCR法检测食管组织中纤维化因子colla-1、TGF-β1及α-SMA mRNA的表达量。结果与对照组小鼠相比,CRS组小鼠体重增加量低于对照组[(8.75±1.69)g vs (12.69±3.16)g],差异有统计学意义(t=3.11,P<0.05)。Masson染色镜下观察可见:CRS组小鼠食管黏膜上皮细胞间明显的纤维化表现,而对照组未见明显异常;免疫组化结果显示:NOX-4阳性着色细胞在大部分标本中均可见到,CRS组小鼠食管组织中阳性着色较对照组染色深且丰富;ELISA法检测结果表明,CRS组小鼠血清中NOX-4表达量明显高于对照组(1.42±0.05 vs 0.49±0.08),差异有统计学意义(t=-27.32,P<0.01)。实时定量RT-PCR实验结果表明,CRS组纤维化标志物(colla-1,TGF-β1,α-SMA)及NOX-4 mRNA表达水平是对照组的(2.43±0.36、2.78±0.13、2.24±0.18、2.48±0.40)倍,差异有统计学意义(t=-11.19、-38.86、-19.90、-10.37,P<0.01)。结论 colla-1、TGF-β1、α-SMA参与心理应激诱导食管纤维化的形成,而氧化应激在食管纤维化产生中具有重要的作用。 展开更多
关键词 应激 心理学 约束 身体的 胶原Ⅰ型 转化生长因子β1 肌动蛋白类 NADPH氧化酶4 纤维化 食管疾病 小鼠
白刺果提取物花色苷通过抑制肝脏纤维化及炎症减轻非酒精性脂肪性肝病 预览
13
作者 杨江霞 白建英 +3 位作者 李付平 张秀芬 徐昭娟 赵冬 《中国病理生理杂志》 CAS CSCD 北大核心 2019年第6期1130-1135,共6页
目的:探讨白刺果提取物花色苷对高脂饮食导致的小鼠非酒精性脂肪肝的治疗作用和潜在机制。方法:高脂饮食建立小鼠非酒精性脂肪肝模型,用花色苷进行干预后,测量小鼠肝脏重量,检测小鼠血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(... 目的:探讨白刺果提取物花色苷对高脂饮食导致的小鼠非酒精性脂肪肝的治疗作用和潜在机制。方法:高脂饮食建立小鼠非酒精性脂肪肝模型,用花色苷进行干预后,测量小鼠肝脏重量,检测小鼠血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)含量;肝脏组织经过HE染色、油红O染色及Masson染色,观察小鼠组织形态、脂滴沉积和胶原纤维增生的情况;Western blot检测肝脏组织中炎症因子肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6及IL-10的水平;免疫组化检测肝脏组织中巨噬细胞、白细胞和单核细胞的浸润情况及肝脏组织趋化因子CCL7和单核细胞趋化蛋白1(MCP-1)的表达。结果:白刺果提取物花色苷能显著抑制由高脂饮食引起的小鼠肝重、ALT、AST、TG、TC及LDL-C的增高;肝脏切片染色显示花色苷能缓解由高脂饮食引起的肝脏纤维化及炎性细胞的浸润;花色苷能降低肝脏中炎症因子TNF-α、IL-1β、IL-6和IL-10及炎性趋化因子CCL7和MCP-1的水平。结论:白刺果提取物花色苷显著缓解由高脂饮食引起的非酒精性脂肪性肝病,其机制主要是通过抑制肝脏中的炎性因子和炎性细胞的浸润并降低炎性趋化因子来实现的。 展开更多
关键词 花色苷 非酒精性脂肪性肝病 炎症 纤维化
在线阅读 免费下载
栀子苷抗肾间质纤维化的作用机制研究 预览
14
作者 孙源博 李桂芹 +4 位作者 孙丽欣 王云枫 关利新 朱敏 贠捷 《中国现代医学杂志》 CAS 2019年第11期8-13,共6页
目的通过转化生长因子-β1(TGF-β1)/Smad-2信号通路探讨栀子苷对肾间质纤维化的影响。方法将SD大鼠随机分为对照组、正常大鼠灌胃组、模型组、30 mg/kg及15 mg/kg栀子苷组,每组8只。复制大鼠单侧输尿管结扎(UUO)肾间质纤维化模型,栀子... 目的通过转化生长因子-β1(TGF-β1)/Smad-2信号通路探讨栀子苷对肾间质纤维化的影响。方法将SD大鼠随机分为对照组、正常大鼠灌胃组、模型组、30 mg/kg及15 mg/kg栀子苷组,每组8只。复制大鼠单侧输尿管结扎(UUO)肾间质纤维化模型,栀子苷组连续给予栀子苷14 d,对照组和模型组灌胃同体积的生理盐水;实验结束后,取大鼠结扎侧的肾脏,用HE染色观察肾间质纤维化病理情况;全自动生化仪检测血肌酐和尿素氮;ELISA检测大鼠血清中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,Western blotting检测TGF-β1和Smad-2蛋白含量。结果与模型组比较,30 mg/kg和15 mg/kg栀子苷组大鼠血清中炎症因子TNF-α和IL-1β含量均降低(P <0.05),且呈剂量依赖性降低,MDA含量降低(P <0.05),SOD活性升高(P <0.05),且随着栀子苷浓度的增加而升高。与对照组比较,模型组肾脏组织中TGF-β1和Smad-2蛋白的表达增加(P <0.05);给予30和15 mg/kg的栀子苷后大鼠肾脏组织中TGF-β1和Smad-2蛋白表达降低(P <0.05)。结论栀子苷具有抗肾间质纤维化作用,其机制可能与通过抑制TGF-β1/Smad信号通路,降低炎症反应及氧化应激损伤有关。 展开更多
关键词 肾病 纤维化 栀子苷 转化生长因子-Β1 Smad-2
在线阅读 下载PDF
The effects of single versus combined therapy using LIM-kinase 2 inhibitor and type 5 phosphodiesterase inhibitor on erectile function in a rat model of cavernous nerve injury-induced erectile dysfunction
15
作者 Min Chui Cho Junghoon Lee +5 位作者 Juhyun Park Sohee Oh Ji Sun Chai Hwancheol Son Jae-Seung Paick Soo Woong Kim 《亚洲男性学杂志:英文版》 SCIE CAS CSCD 2019年第5期493-500,共8页
We aimed to determine whether combination of LIM-kinase 2 inhibitor(LIMK2i)and phosphodiesterase type-5 inhibitor(PDE5i)could restore erectile function through suppressing cavernous fibrosis and improving caver nous a... We aimed to determine whether combination of LIM-kinase 2 inhibitor(LIMK2i)and phosphodiesterase type-5 inhibitor(PDE5i)could restore erectile function through suppressing cavernous fibrosis and improving caver nous apoptosis in a rat model of cavernous nerve crush injury(CNCI).Seventy 12-week-old Sprague-Dawley rats were equally distributed into five groups as follows:(1)sham surgery(Group S),(2)CNCI(Group I),(3)CNCI treated with daily intraperitoneal administration of 10.0 mg kg^-1 LIMK2i(Group I+L),(4)daily oral administration of 20.0 mg kg-1 udenafil,PDE5i(Group I+U),and(5)combined administration of 10.0 mg kg^-1 LIMK2i and 20.0 mg kg^-1 udenafil(Group I+L+U).Rats in Groups I+L,I+U,and I+L+U were treated with respective regimens for 2 weeks after CNCI.At 2 weeks after surgery,erectile response was assessed using electrostimulation.Penile tissues were processed for histological studies and western blot.Group I showed lower intracavernous pressure(ICP)/mean arterial pressure(MAP),lower area under the curve(AUC)/MAP,decreased immunohistochemical staining for alpha-smooth muscle(SM)actin,higher apoptotic index,lower SM/collagen ratio,increased phospho-LIMK2-positive fibroblasts,decreased protein kinase B/endothelial nitric oxide synthase(Akt/eNOS)phosphorylation,increased LIMK2/cofilin phosphorylation,and increased protein expression of fibronectin,compared to Group S.In all three treatment groups,erectile responses,protein expression of fibronectin,and SM/collagen ratio were improved.Group I+L+U showed greater improvement in erectile response than Group I+L.SM content and apoptotic index in Groups I+U and I+L+U were improved compared to those in Group I.However,Group I+L did not show a significant improvement in SM content or apoptotic index.The number of phospho-LIMK2-positive fibroblasts was normalized in Groups I+L and I+L+U,but not in Group I+U.Akt/eNOS phosphorylation was improved in Groups I+U and I+L+U,but not in Group I+L.LIMK2/cofilin phosphorylation was improved in Groups I+L and I+L+U,but not in Group 展开更多
关键词 APOPTOSIS erectile dysfunction FIBROSIS LIM kinase PHOSPHODIESTERASE
心力衰竭新型生物标志物的临床意义 预览
16
作者 文霄敏 赵羽 刘卫生 《医学综述》 2019年第17期3438-3441,3446共5页
血浆生物标志物是诊断和预测心力衰竭的常用工具。临床实践中,心脏特异性生物标志物只有利钠肽类和高敏感性肌钙蛋白得到广泛应用。半乳糖凝集素-3和可溶性基质裂解素-2是美国心脏病学会/美国心脏协会心力衰竭指南中唯一纳入的新型心力... 血浆生物标志物是诊断和预测心力衰竭的常用工具。临床实践中,心脏特异性生物标志物只有利钠肽类和高敏感性肌钙蛋白得到广泛应用。半乳糖凝集素-3和可溶性基质裂解素-2是美国心脏病学会/美国心脏协会心力衰竭指南中唯一纳入的新型心力衰竭生物标志物,但是临床价值还需进一步证明。由于各种标志物的特异性不同,在心力衰竭发生发展过程中的作用及临床意义不同,而单用某一种标志物诊断心力衰竭比较片面,临床上可通过联合监测多种生物标志物来提高诊断特异性。 展开更多
关键词 心力衰竭 生物标志物 纤维化 细胞损伤 炎症
在线阅读 下载PDF
Role of the Notch Signaling Pathway in Fibrosis of Denervated Skeletal Muscle 预览
17
作者 Fei FENG Lu SHAN +2 位作者 Jing-xiu DENG Ling-li LUO Qi-shun HUANG 《当代医学科学(英文)》 SCIE CAS 2019年第3期419-425,共7页
In order to investigate the role of the Notch signaling pathway in skeletal muscle fibrosis after nerve injury, 60 Sprague-Dawley rats were selected and divided randomly into a control and two experimental groups. Gro... In order to investigate the role of the Notch signaling pathway in skeletal muscle fibrosis after nerve injury, 60 Sprague-Dawley rats were selected and divided randomly into a control and two experimental groups. Group A served as controls without any treatment. Rats in groups B were injected intraperitoneally with 0.2 mL PBS and those in group C were injected intraperitoneally with 0.2 mL PBS+100 ymol/L, 0.2 mL N-[N-(3,5-difluorophenacetyl)-l-alanyl]- S-phenylglycine t-butyl ester (DAPT, a gamma-secretase inhibitor that suppresses Notch signaling) respectively, on postoperative days 1, 3, 7, 10, and 14 in a model of denervation-induced skeletal muscle fibrosis by right sciatic nerve transection. Five rats from each group were euthanized on postoperative days 1, 7, 14, and 28 to collect the right gastrocnemii, and hematoxylin and eosin (HE) staining, immunohistochemistry test, real-time PCR, and Western blotting were performed to assess connective tissue hyperplasia and fibroblast density as well as expression of Notch 1, Jagged 1, and Notch downstream molecules Hes 1 and collagen I (COL I) on day 28. There was no significant difference in HE-stained fibroblast density between group B and C on postoperative day 1. However, fibroblast density was significantly higher in group B than in group C on postoperative days 7, 14, and 28. Notch 1, Jagged 1, Hes 1, and COL I proteins in the gastrocnemius were expressed at very low levels in group A but at high levels in group B. Expression levels of these proteins were significantly lower in group C than in group B (P<0.05), but they were higher in group C than in group A (P<0.05) on postoperative day 28. We are led to conclude that locking the Notch signaling pathway inhibits fibrosis progression of denervated skeletal muscle. Thus, it may be a new approach for treatment of fibrosis of denervated skeletal muscle. 展开更多
关键词 NOTCH signaling pathway sciatic nerve skeletal muscle FIBROSIS N-[N-(3 5- difluorophenacetyl)-l-alanyl]-S-phenylglycine T-BUTYL ester NOTCH 1 JAGGED 1 Hes 1 collagen I denervated muscular atrophy
在线阅读 下载PDF
Human tissue kallikreiprotects against the development of erectile dysfunction in a rat model of hyperhomocysteinemia
18
作者 Kai Cui Yang Luan +5 位作者 Zhe Tang Chuan-Chang Li Tao Wang Shao-Gang Wang Zhong Chen Ji-Hong Liu 《亚洲男性学杂志:英文版》 SCIE CAS CSCD 2019年第5期508-515,共8页
The aim of this study was to investigate the mechanism by which a diet inducing high hyperhomocysteinemia(HHcy)leads to the deterioration of erectile function in rats and whether this is inhibited by expression of the... The aim of this study was to investigate the mechanism by which a diet inducing high hyperhomocysteinemia(HHcy)leads to the deterioration of erectile function in rats and whether this is inhibited by expression of the human tissue kallikrein-1(hKLKl)gene.We established a rat model of HHcy by feeding methionine(Met)-rich diets to male Sprague-Dawley(SD)rats.Male wild-type SD rats(WTRs)and transgenic rats harboring the hKLKl gene(TGRs)were fed a normal diet until 10 weeks of age.Then,30 WTRs were randomly divided into three groups as follows:the control(n=10)group,the low-dose(4%Met,n=10)group,and the high-dose(7%Met,n=10)group.Another 10 age-matched TGRs were fed the high-dose diet and designated as the TGR+7%Met group.After 30 days,in all four groups,erectile function was measured and penile tissues were harvested to determine oxidative stress,endothelial cell content,and penis fibrosis.Compared with the 7%Met group,the TGR+7%Met group showed diminished HHcy-induced erectile dysfunction(ED),indicating the improvement caused by hKLKl.Regarding corpus cavernosum endothelial cells,hKLKl preserved endothelial cell-cell junctions and endothelial cell content,and activated protein kinase B/endothelial nitric oxide synthase(Akt/eNOS)signaling.Fibrosis assessment indicated that hKLKl preserved normal penis structure by inhibiting apoptosis in the corpus cavernosum smooth muscle cells.Taken together,these findings showed that oxidative stress,impaired corpus cavernosum endothelial cells,and severe penis fibrosis were involved in the in duction of ED by HHcy in rats,whereas hKLKl preserved erectile function by inhibiting these pathophysiological changes. 展开更多
关键词 endothelial cell erectile function FIBROSIS human tissue kallikrein-1 HYPERHOMOCYSTEINEMIA oxidative stress
Persistent elevation of fibrosis biomarker cartilage oligomeric matrix protein following hepatitis C virus eradication 预览
19
作者 Kristofer Andréasson Goran Jonsson +1 位作者 Roger Hesselstrand Hans Norrgren 《世界肝病学杂志:英文版(电子版)》 2019年第3期330-334,共5页
Serum levels of cartilage oligomeric matrix protein (COMP) has been presented as a biomarker of liver fibrosis in several cross-sectional studies. COMP is also an essential mediator in carcinoma development and has al... Serum levels of cartilage oligomeric matrix protein (COMP) has been presented as a biomarker of liver fibrosis in several cross-sectional studies. COMP is also an essential mediator in carcinoma development and has also been associated with hepatocellular carcinoma. We present a prospective analysis of this biomarker in 38 patients with chronic hepatitis C who were subject to eradication therapy with direct acting antivirals. We confirm previous studies associating COMP elevation with liver cirrhosis. We also show how viral levels are correlated with COMP at baseline. In our prospective analysis, we report that successful eradication of hepatitis C results in improvement in liver stiffness and laboratory liver function tests at 1 year follow-up. In contrast, median COMP-levels remain unchanged during the study period. We conclude that the biomarker potential of COMP in the prospective evaluation of liver diseases, remains to be elucidated. 展开更多
关键词 Hepatitis C Chronic CARTILAGE oligomeric matrix protein FIBROSIS
在线阅读 免费下载
Emodin alleviates cardiac fibrosis by suppressing activation of cardiac fibroblasts via upregulating metastasis associated protein 3
20
作者 Dan Xiao Yue Zhang +12 位作者 Rui Wang Yujie Fu Tong Zhou Hongtao Diao Zhixia Wang Yuan Lin Zhange Li Lin Wen Xujuan Kang Philipp Kopylov Dmitri Shchekochikhin Yong Zhang Baofeng Yang 《药学学报:英文版》 CSCD 2019年第4期724-733,共10页
Excess activation of cardiac fibroblasts inevitably induces cardiac fibrosis. Emodin has been used as a natural medicine against several chronic diseases. The objective of this study is to determine the effects of emo... Excess activation of cardiac fibroblasts inevitably induces cardiac fibrosis. Emodin has been used as a natural medicine against several chronic diseases. The objective of this study is to determine the effects of emodin on cardiac fibrosis and the underlying molecular mechanisms. Intragastric administration of emodin markedly decreased left ventricular wall thickness in a mouse model of pathological cardiac hypertrophy with excess fibrosis induced by transaortic constriction(TAC) and suppressed activation of cardiac fibroblasts induced by angiotensin II(AngII). Emodin upregulated expression of metastasis associated protein 3(MTA3) and restored the MTA3 expression in the setting of cardiac fibrosis. Moreover, overexpression of MTA3 promoted cardiac fibrosis;in contrast, silence of MTA3 abrogated the inhibitory effect of emodin on fibroblast activation. Our findings unraveled the potential of emodin to alleviate cardiac fibrosis via upregulating MTA3 and highlight the regulatory role of MTA3 in the development of cardiac fibrosis. 展开更多
关键词 EMODIN CARDIAC FIBROSIS MTA3 ANGIOTENSIN CARDIAC FIBROBLAST Mouse
上一页 1 2 250 下一页 到第
使用帮助 返回顶部 意见反馈