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Petroleum Migration Characteristics in the Northeastern Part of the Baiyun Depression,Pearl River Mouth Basin,South China Sea 认领
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作者 ZENG Jianhui WANG Chen +5 位作者 GUO Shuai YU Yixin ZHANG Zhongtao YANG Haizhang ZHAO Zhao SUN Rui 《地质学报:英文版》 SCIE CAS 2021年第1期208-231,共24页
This paper investigates the origin and migration characteristics of petroleum in the northeastern part of the Baiyun Depression,Pearl River Mouth Basin(PRMB).The discovered petroleum in the study area is mainly locate... This paper investigates the origin and migration characteristics of petroleum in the northeastern part of the Baiyun Depression,Pearl River Mouth Basin(PRMB).The discovered petroleum in the study area is mainly located in the Lower Zhujiang Member(N_(1)z^(2))and mainly originated from the Enping Formation source rocks in the eastern sag.Active faults(vertical migration)and N_(1)z^(2)sandstones(lateral migration)acted as the petroleum migration systems.The fault activities in the Dongsha event controlled the episodic petroleum migration.Fractures in the fault zones provided effective conduits,and overpressure was the driving force.The vertical migration could not cross the fault zones laterally.The petroleum injection areas in the carrier beds were the contact zones of petroleum-migration faults and carrier beds.The lateral migration was steady-state migration,and buoyancy was the driving force.The migration pathways in the carrier beds were controlled by the structural morphology.Secondary petroleum migration in the study area could be divided into two parts:vertical migration along the fractures in the fault zones and lateral migration through preferential petroleum migration pathways(PPMPs)in the carrier beds.The petroleum migration behaviors,including migrating direction,driving force,and migration pattern,in the faults and sandstone carrier beds were quite different.This study provides a typical example for comprehending secondary migration processes and has great importance for determining future exploration targets in the deep-water area of the PRMB. 展开更多
关键词 secondary petroleum migration episodic petroleum migration preferential petroleum migration pathways(PPMPs) Baiyun Depression Pearl River Mouth Basin
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Distribution of 137Cs and 60Co in plough layer of farmland: Evidenced from a lysimeter experiment using undisturbed soil columns 认领
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作者 Wenxiang LIU Yong LI +3 位作者 Hanqing YU Surinder SAGGAR Daozhi GONGI Qiong ZHANG 《土壤圈:英文版》 SCIE CAS 2021年第1期180-190,共11页
Radionuclide fallout during nuclear accidents on the land may impair the atmosphere, contaminate farmland soils and crops, and can even reach the groundwater. Previous research focused on the field distribution of dep... Radionuclide fallout during nuclear accidents on the land may impair the atmosphere, contaminate farmland soils and crops, and can even reach the groundwater. Previous research focused on the field distribution of deposited radionuclides in farmland soils, but details of the amounts of radionuclides in the plough layer and the changes in their proportional distribution in the soil profile with time are still inadequate. In this study, a lysimeter experiment was conducted to determine the vertical migration of 137Cs and 60Co in brown and aeolian sandy soils, collected from the farmlands adjoining Shidaowan Nuclear Power Plant(NPP) in eastern China, and to identify the factors influencing their migration depths in soil. At the end of the experiment(800 d), >96% of added 137Cs and 60Co were retained in the top 0–20 cm soil layer of both soils;very little 137Cs or 60Co initially migrated to 20–30 cm, but their amounts at this depth increased with time. The migration depth of 137Cs was greater in the aeolian sandy soil than in the brown soil during 0–577 d, but at the end of the experiment, 137Cs migrated to the same depth(25 cm) in both soils. Three phases on the vertical migration rate(v) of 60Co in the aeolian sandy soil can be identified: an initial rapid movement(0–355 d, v = 219 ± 17 mm year-1), followed by a steady movement(355–577 d, v = 150 ± 24 mm year-1) and a very slow movement(577–800 d, v = 107 ± 7 mm year-1). In contrast, its migration rate in the brown soil(v = 133 ± 17 mm year-1) was steady throughout the 800-d experimental period. The migration of both 137Cs and 60Co in the two soils appears to be regulated by soil clay and silt fractions that provide most of the soil surface area, soil organic carbon(SOC), and soil pH, which were manifested by the solid-liquid distribution coefficient of 137Cs and 60Co. The results of this study suggest that most 137Cs and 60Co remained within the top layer(0–20 cm depth) of farmland soils following a simulated NPP accident, and little reached the 展开更多
关键词 aeolian sandy soil brown soil deposited radionuclide migration depth RADIONUCLIDE vertical migration rate
Data Migration Need, Strategy, Challenges, Methodology, Categories, Risks, Uses with Cloud Computing, and Improvements in Its Using with Cloud Using Suggested Proposed Model (DMig 1) 认领
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作者 Abou_el_ela Abdou Hussein 《信息安全(英文)》 2021年第1期79-103,共25页
Data Migration is a multi-step process that begins with analyzing old data and culminates in data uploading and reconciliation in new applications. With the rapid growth of data, organizations constantly need to migra... Data Migration is a multi-step process that begins with analyzing old data and culminates in data uploading and reconciliation in new applications. With the rapid growth of data, organizations constantly need to migrate data. Data migration can be a complex process as testing must be done to ensure data quality. Migration also can be very costly if best practices are not followed and hidden costs are not identified in the early stage. <span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">O</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">n the other hand</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">,</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> many organizations today instead of buying IT equipment (hardware and/or software) and managing it themselves, they prefer to buy services from IT service providers. The number of service providers is increasing dramatically and the cloud is becoming the preferred tool for more cloud storage services. However, as more information and personal data are transferred to the cloud, to social media sites, DropBox, Baidu WangPan, etc., data security and privacy issues are questioned. So, academia and industry circles strive to find an effective way to secure data migration in the cloud. Various resolving methods and encryption techniques have been implemented. In this work, we will try to cover many important points in data migration as Strategy, Challenges, Need, methodology, Categories, Risks, and Uses with Cloud computing. Finally, we discuss data migration security and privacy challenge and how to solve this problem by making improvements in it’s using with Cloud through suggested proposed model that enhances data security and privacy by gathering Advanced Encryption Standard-256 (ATS256), D 展开更多
关键词 CLOUD Organizations Migration Data Quality Advanced Encryption Standard
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Migration of invasive Spodoptera frugiperda(Lepidoptera:Noctuidae)across the Bohai Sea in northern China 认领 被引量:3
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作者 JIA Hui-ru GUO Jiang-long +4 位作者 WU Qiu-lin HU Chao-xing LI Xiao-kang ZHOU Xian-yong WU Kong-ming 《农业科学学报:英文版》 SCIE CAS 2021年第3期685-693,共9页
The fall armyworm(FAW),Spodoptera frugiperda(J.E.Smith),is an important agricultural pest with a strong migratory ability.While the species is native to the Americas,it has recently invaded China,ravaging crops in man... The fall armyworm(FAW),Spodoptera frugiperda(J.E.Smith),is an important agricultural pest with a strong migratory ability.While the species is native to the Americas,it has recently invaded China,ravaging crops in many provinces(autonomous regions,municipalities).Its long-distance migration,which is critical to understand for pest management programs,has been well studied in its native region.In other regions however,the moth’s migration patterns have not yet been characterized.Here,the migratory behavior of FAW populations in China was studied on an isolated island located in the center of the Bohai Strait from spring to autumn 2019,the year in which FAW first reached this region,by using searchlight trapping,stable carbon isotopes and trajectory simulation.The main results were summarized as follows:(i)The number of FAW moths caught by searchlight trapping provided direct evidence that the species migrated across the Bohai Sea.(ii)Species identification was confirmed by both morphology and molecular methods,and only the"corn-strain"that preferentially infests maize and sorghum was found in the collections.(iii)Stable carbon isotope measurements showed that up to 95.07%of captured moths displayed the C4 isotope signature,thus providing additional evidence that this species is a migrant as there are no major C4 plants at the trapping site.(iv)Backward trajectory analysis indicated that the pest now threatens to expand its range into the agriculturally important region of Northeast China,and thus authorities in this region should be vigilant to the threat posed by this species.Together,these findings add to our knowledge concerning the occurrence of FAW in northern China,and will help us to develop sustainable and effective monitoring,forecasting,and pest management strategies. 展开更多
关键词 Spodoptera frugiperda overseas migration searchlight trapping host strains stable carbon isotopes migration trajectory
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Population migration across the Qinghai-Tibet Plateau:Spatiotemporal patterns and driving factors 认领
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作者 WANG Nan WANG Huimeng +3 位作者 DU Yunyan YI Jiawei LIU Zhang TU Wenna 《地理学报:英文版》 SCIE 2021年第2期195-214,共20页
Developing a comprehensive understanding of inter-city interactions is crucial for regional planning.We therefore examined spatiotemporal patterns of population migration across the Qinghai-Tibet Plateau(QTP)using mig... Developing a comprehensive understanding of inter-city interactions is crucial for regional planning.We therefore examined spatiotemporal patterns of population migration across the Qinghai-Tibet Plateau(QTP)using migration big data from Tencent for the period between 2015 and 2019.We initially used decomposition and breakpoint detection methods to examine time-series migration data and to identify the two seasons with the strongest and weakest population migration levels,between June 18th and August 18th and between October 8th and February 15th,respectively.Population migration within the former period was 2.03 times that seen in the latter.We then used a variety of network analysis methods to examine population flow directions as well as the importance of each individual city in migration.The two capital cities on the QTP,Lhasa and Xining,form centers for population migration and are also transfer hubs through which migrants from other cities off the plateau enter and leave this region.Data show that these two cities contribute more than 35%of total population migration.The majority of migrants tend to move within the province,particularly during the weakest migration season.We also utilized interactive relationship force and radiation models to examine the interaction strength and the radiating energy of each individual city.Results show that Lhasa and Xining exhibit the strongest interactions with other cities and have the largest radiating energies.Indeed,the radiating energy of the QTP cities correlates with their gross domestic product(GDP)(Pearson correlation coefficient:0.754 in the weakest migration season,WMS versus 0.737 in the strongest migration season,SMS),while changes in radiating energy correlate with the tourism-related revenue(Pearson correlation coefficient:0.685).These outcomes suggest that level of economic development and level of tourism are the two most important factors driving the QTP population migration.The results of this analysis provide critical clarification guidance regarding h 展开更多
关键词 Qinghai-Tibet Plateau population migration migration big data flow radiation model spatiotemporal interaction mode
miR-28-5p对结肠癌细胞增殖和迁移的影响 认领
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作者 朱庭沛 桂裕昌 +3 位作者 覃慧晴 陈建泉 唐云 许建文 《中国医学工程》 2021年第2期21-25,共5页
目的探究miR-28-5p对结肠癌细胞增殖和迁移能力的影响。方法采用qRT-PCR检测miR-28-5p在正常结肠细胞NCM460与结肠癌细胞HCT116、SW480、SW620中表达量的差异。将HCT116、SW480、SW620各分为三组:对照组,过表达组,沉默组。对照组不予特... 目的探究miR-28-5p对结肠癌细胞增殖和迁移能力的影响。方法采用qRT-PCR检测miR-28-5p在正常结肠细胞NCM460与结肠癌细胞HCT116、SW480、SW620中表达量的差异。将HCT116、SW480、SW620各分为三组:对照组,过表达组,沉默组。对照组不予特殊处理,其余两组分别转染miR-28-5p mimic和miR-28-5p inhibitor,采用qRT-PCR验证转染效率,采用CCK8法检测三组细胞的增殖能力,采用划痕实验检测三组细胞的的迁移能力。结果miR-28-5p在结肠癌细胞HCT116、SW480、SW620中低表达,与正常结肠细胞NCM460比较差异有统计学意义(P<0.05)。与对照组相比,过表达组细胞增殖和迁移能力均降低(P<0.05),沉默组细胞增殖和迁移能力均增强(P<0.05)。结论miR-28-5p在结肠癌细胞中低表达且有调控结肠癌细胞增殖和迁移的作用。 展开更多
关键词 miR-28-5p 结肠癌 增殖 迁移
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lncRNA LINC02418通过调控miR-940表达对肝癌细胞增殖、迁移、侵袭和凋亡的影响 认领
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作者 方燕 陈建欧 郑旭旭 《世界华人消化杂志》 CAS 2021年第2期65-74,共10页
背景lncRNA LINC02418在非小细胞肺癌、肺腺癌和结直肠癌等肿瘤中表达上调,促进肿瘤的发展进程.但是,LINC02418对肝癌发生发展的影响和机制还未知.LncBase Predicted v.2靶基因预测显示,LINC02418可能靶向结合miR-940.本研究假设LINC02... 背景lncRNA LINC02418在非小细胞肺癌、肺腺癌和结直肠癌等肿瘤中表达上调,促进肿瘤的发展进程.但是,LINC02418对肝癌发生发展的影响和机制还未知.LncBase Predicted v.2靶基因预测显示,LINC02418可能靶向结合miR-940.本研究假设LINC02418可靶向调控miR-940影响肝癌细胞增殖、迁移、侵袭及凋亡,进而影响肝癌发展进程.目的探讨lncRNA LINC02418对肝癌细胞增殖、迁移、侵袭和凋亡的影响及作用机制.方法RT-qPCR检测肝癌组织和对应癌旁组织中LINC02418和miR-940表达水平.分别转染LINC02418小干扰RNA、miR-940模拟物至肝癌细胞HCCLM3,RTqPCR检测转染效果,CCK-8、Transwell、流式细胞术和蛋白印迹法分别检测下调LINC02418表达或上调miR-940表达对HCCLM3细胞活性、迁移和侵袭、凋亡及CyclinD1、p21、MMP-2、MMP-9、Bcl-2和Bax蛋白表达的影响.双荧光素酶报告基因实验验证miR-940与LINC02418调控关系.结果与癌旁组织比较,肝癌组织中LINC02418表达水平升高(P<0.05),miR-940表达水平降低(P<0.05).下调LINC02418或上调miR-940表达降低了HCCLM3细胞活性、迁移和侵袭数及CyclinD1、MMP-2、MMP-9和Bcl-2的蛋白表达(P<0.05),而提高了细胞凋亡率、p21和Bax的蛋白表达(P<0.05).LINC02418靶向负调控miR-940表达.下调miR-940表达逆转了下调LINC02418表达对HCCLM3细胞增殖、迁移、侵袭和凋亡的影响.结论LINC02418在肝癌组织中表达升高,下调其表达可能通过靶向上调miR-940抑制肝癌细胞的恶性生物学行为,可作为肝癌治疗的分子靶点. 展开更多
关键词 LINC02418 miR-940 肝癌 细胞增殖 迁移 侵袭 凋亡
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A novel bio‑active microsphere for meniscus regeneration via inducing cell migration and chondrocyte differentiation 认领
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作者 Hongyao Xu He Huang +3 位作者 Xiangjie Zou Pengcheng Xia Warren A.L.S.Foon Jinwen Wang 《生物设计与制造(英文)》 SCIE EI 2021年第2期203-221,共19页
Abstract Meniscus injury is a common disease in clinic.If it was not treated in time,it leads to osteoarthritis which brings unbearable pain and heavy economic burden to the patients.At present,meniscectomy and menisc... Abstract Meniscus injury is a common disease in clinic.If it was not treated in time,it leads to osteoarthritis which brings unbearable pain and heavy economic burden to the patients.At present,meniscectomy and meniscus suture are widely used in the treatment for meniscus injury.Nevertheless,It is not ideal for poor self-healing ability of meniscus.The recruitment of endogenous stem cells is an attractive option for wounded meniscus healing.Fully reduced high-mobility group box 1 protein(HMGB1)can accelerate the regeneration of multiple tissues by endogenous stem cell activation,migration and differentiation.Kartogenin(KGN)has shown to induce the chondrogenesis of the stem cells.However,no study has explored such effects of HMGB1 and KGN in wounded meniscus healing.Therefore,in order to improve the regeneration of meniscus,we intend to use a novel bioactive microsphere which was developed by combining fully reduced high mobility group box 1(frHMGB1)and kartogenin(KGN)with alginate gel which slowly release high concentrations of HMGB1 and KGN to activate rat bone marrow stem cells(BMSCs)and promote cell proliferation.The results showed that this HMGB1–KGN microsphere released and kept high concentrations of HMGB1 and KGN in the wound area for more than 2 weeks.In vitro experimental results showed that the HMGB1–KGN microsphere can promote cell proliferation via recruiting rat bone marrow stem cells(BMSCs)and activating the BMSCs from G_(0) to G_(Alert) stage as evidenced by cell migration testing and 5-bromo-2′-deoxyuridine(BrdU)incorporation assay.In vivo results indicated that this HMGB-KGN microsphere can recruit GFP-labeled BMSCs from tail vein to wounded meniscus and induce these GFP-labeled BMSCs to differentiate into chondrocytes.Our results demonstrated that the HMGB1–KGN-containing bioactive microsphere induced cell migration in vitro and recruited the cells to wound area to promote wounded rat meniscus healing in vivo. 展开更多
关键词 Fully reduced HMGB1(frHMGB1) Kartogenin Alginate microsphere Cell migration Meniscus regeneration
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piRNA NU13对肾母细胞瘤细胞增殖、凋亡、迁移和侵袭的影响 认领
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作者 张朝霞 王璋 +5 位作者 金黎明 谭小军 王钊颖 沈炼桔 魏光辉 何大维 《南方医科大学学报》 CAS 北大核心 2021年第2期184-192,共9页
目的探讨piwil2诱导的肿瘤样干细胞(piwil2-iCSCs)来源的差异piRNA NU13对肾母细胞瘤细胞(G401)生物学行为的影响。方法RT-qPCR检测G401中piRNANU13及NOP56的表达情况;通过脂质体转染技术,在肾母细胞瘤细胞株G401中过表达及抑制piRNANU... 目的探讨piwil2诱导的肿瘤样干细胞(piwil2-iCSCs)来源的差异piRNA NU13对肾母细胞瘤细胞(G401)生物学行为的影响。方法RT-qPCR检测G401中piRNANU13及NOP56的表达情况;通过脂质体转染技术,在肾母细胞瘤细胞株G401中过表达及抑制piRNANU13,并用RT-qPCR技术验证转染后G401细胞中piRNANU13的表达量;通过CCK8检测转染后G401细胞增殖情况,流式细胞术检测细胞凋亡能力,划痕实验和Transwell实验分别检测细胞迁移和侵袭能力的改变;通过双荧光素酶实验检测NOP56与piRNA NU13的结合情况;通过Western blot检测MMP2、MMP9、BAX、Bcl2、NOP56蛋白表达情况。结果在人肾母细胞瘤细胞G401中piRNANU13表达水平低于肾小管上皮细胞HK2(P<0.05);NOP56在G401及肾母细胞瘤组织均高表达(P<0.05);在G401中上调piRNANU13表达能够抑制肿瘤细胞的增殖、迁移、侵袭,并促进肿瘤细胞凋亡(P<0.05);过表达piRNANU13可抑制MMP2、MMP9及Bcl2的表达水平,促进BAX表达(P<0.05);piRNANU13与预测靶基因NOP56非直接结合,但可间接调控NOP56的表达。结论piRNANU13在肾母细胞瘤中低表达,NOP56在肾母细胞瘤中高表达,piNU13可能间接调控NOP56表达从而抑制肿瘤细胞增殖、迁移、侵袭,促进其凋亡。 展开更多
关键词 piwil2-iCSCs PIRNA 人肾母细胞瘤 增殖 凋亡 迁移 侵袭
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长链非编码RNA DLX6-AS1通过靶基因miR-103a-3p调控宫颈癌SiHa细胞的分子机制 认领
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作者 周倩 郝硕月 张海燕 《西部医学》 2021年第1期44-49,共6页
目的探讨长链非编码RNA DLX6-AS1通过靶基因miR-103a-3p调控宫颈癌SiHa细胞增殖、迁移、侵袭和凋亡的影响及其作用机制。方法培养正常宫颈细胞Ect1/E6E7和宫颈癌细胞株HeLa、SiHa、C33a、Caski。将SiHa随机分为对照(NC)组、si-con组、si... 目的探讨长链非编码RNA DLX6-AS1通过靶基因miR-103a-3p调控宫颈癌SiHa细胞增殖、迁移、侵袭和凋亡的影响及其作用机制。方法培养正常宫颈细胞Ect1/E6E7和宫颈癌细胞株HeLa、SiHa、C33a、Caski。将SiHa随机分为对照(NC)组、si-con组、si-DLX6-AS1组、PCDNA组、PCDNA-DLX6-AS1组、miR-con组、miR-103a-3p组、si-DLX6-AS1+anti-miR-con组、si-DLX6-AS1+anti-miR-103a-3p组。RT-qPCR检测各组细胞中miR-103a-3p和DLX6-AS1表达水平;蛋白质印迹(Western Blot)法检测蛋白表达水平;四甲基偶氮唑盐比色法(MTT)检测细胞活性;流式细胞术检测细胞凋亡;Transwell检测细胞迁移和侵袭;双荧光素酶报告实验检测DLX6-AS1和miR-103a-3p的靶向关系。结果与正常宫颈细胞Ect1/E6E7相比,宫颈癌细胞HeLa、SiHa、C33a、Caski中DLX6-AS1表达水平升高,miR-103a-3p表达水平降低,差异均有统计学意义(P<0.05)。干扰DLX6-AS1表达或miR-103a-3p过表达后,细胞活性降低,细胞凋亡率升高,迁移、侵袭数量减少,CyclinD1、Bcl-2、MMP-2、MMP-9表达水平降低,Bax、Cleaved caspase-3表达水平升高,差异均有统计学意义(均P<0.05)。同时抑制miR-103a-3p和DLX6-AS1表达后,宫颈癌SiHa中MMP-2、MMP-9、CyclinD1、Bcl-2表达水平升高,Bax、Cleaved caspase-3表达水平降低,细胞活性升高,细胞凋亡率降低,差异均有统计学意义(均P<0.05)。DLX6-AS1靶向调控miR-103a-3p的表达。结论干扰DLX6-AS1表达可抑制宫颈癌细胞增殖、迁移、侵袭,促进细胞凋亡,其机制可能与miR-103a-3p表达有关,可能为宫颈癌的治疗提供新思路和新靶点。 展开更多
关键词 宫颈癌 DLX6-AS1 miR-103a-3p 增殖 迁移 侵袭 凋亡
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Flight activity promotes reproductive processes in the fall armyworm,Spodoptera frugiperda 认领 被引量:2
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作者 GE Shi-shuai HE Wei +3 位作者 HE Li-mei YAN Ran ZHANG Hao-wen WU Kong-ming 《农业科学学报:英文版》 SCIE CAS 2021年第3期727-735,共9页
The fall armyworm(FAW),Spodoptera frugiperda(Lepidoptera:Noctuidae),has invaded many countries in Africa and Asia since 2016,posing a major threat to world food security.Long-distance migration and strong reproductive... The fall armyworm(FAW),Spodoptera frugiperda(Lepidoptera:Noctuidae),has invaded many countries in Africa and Asia since 2016,posing a major threat to world food security.Long-distance migration and strong reproductive ability form the biological basis of its rapid population expansion,but the relationship between the flight and reproduction of FAW has not been studied in depth.Here,an empirical assessment of this relationship in an invasive FAW population in China found that 1-3-day-old adults which had undergone 10-h tethered flights had a significantly shorter pre-oviposition period and greater oviposition synchronization,but did not show any differences in fecundity,oviposition period,mating percentage or other reproductive variables.Further studies on moths after 1.25-15-h tethered flights indicated that the reproductive process of adults could be fully triggered by flight activity longer than 2.5 h.Dissection of the reproductive organs also showed that tethered flight promoted ovarian and testicular development of FAW.These results show that appropriate moth flight activity significantly speeds up the reproductive process of FAW,which increases our knowledge on its migratory biology in relation to regional outbreaks. 展开更多
关键词 FLIGHT REPRODUCTION MIGRATION invasion biology movement ecology
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LncRNA LINC01224/miR-513b-5p对结肠癌细胞SW1116增殖、迁移及侵袭的影响 认领
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作者 张兆辉 王利民 《世界华人消化杂志》 CAS 2021年第1期7-14,共8页
背景我国结肠癌发病率与死亡率逐年上升,目前结肠癌的发病机制尚未阐明,LncRNA在结肠癌等肿瘤发生过程中可发挥重要调控作用,其主要通过竞争性结合miRNA而发挥作用,已知LncRNA LINC01224在肿瘤中可能发挥癌基因作用,但LINC01224在结肠... 背景我国结肠癌发病率与死亡率逐年上升,目前结肠癌的发病机制尚未阐明,LncRNA在结肠癌等肿瘤发生过程中可发挥重要调控作用,其主要通过竞争性结合miRNA而发挥作用,已知LncRNA LINC01224在肿瘤中可能发挥癌基因作用,但LINC01224在结肠癌形成及发展过程中的作用机制尚未阐明.目的探讨LncRNA LINC01224/miR-513b-5p对结肠癌细胞SW1116增殖、迁移、侵袭的影响及其可能作用机制.方法采用qRT-PCR法检测癌旁组织与结肠癌组织中LINC01224、miR-513b-5p的表达量;si-NC、si-LINC01224、si-LINC01224与anti-miR-NC、si-LINC01224与anti-miR-513b-5p分别转染入人结肠癌细胞SW1116;采用qRT-PCR法检测SW1116细胞中LINC01224、miR-513b-5p的表达量;采用MTT实验检测细胞存活率;应用流式细胞仪检测细胞周期;采用Transwell小室实验检测细胞迁移及侵袭;双荧光素酶报告实验检测LINC01224与miR-513b-5p的靶向关系;Western blot法检测E-cadherin、N-cadherin蛋白表达量.结果与癌旁组织比较,结肠癌组织中LINC01224的表达水平升高(P<0.05),miR-513b-5p的表达水平降低(P<0.05);与si-NC组比较,si-LINC01224组细胞存活率降低(P<0.05),G0-G1期细胞比例升高(P<0.05),S期细胞比例降低(P<0.05),迁移及侵袭细胞数减少(P<0.05),E-cadherin蛋白水平升高(P<0.05),N-cadherin蛋白水平降低(P<0.05);双荧光素酶报告实验证实LINC01224可靶向结合miR-513b-5p;与si-LINC01224+anti-miR-NC组比较,si-LINC01224+antimiR-513b-5p组细胞存活率升高(P<0.05),G0-G1期细胞比例降低(P<0.05),S期细胞比例升高(P<0.05),迁移及侵袭细胞数增多(P<0.05),E-cadherin蛋白水平降低(P<0.05),N-cadherin蛋白水平升高(P<0.05).结论干扰LINC01224表达可通过上调miR-513b-5p而减弱结肠癌细胞增殖、迁移及侵袭能力,并可诱导细胞周期阻滞于G0-G1期. 展开更多
关键词 LncRNA LINC01224 miR-513b-5p 结肠癌 增殖 迁移 侵袭
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板块俯冲中水分运移过程及其地球物理含义 认领
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作者 盛俭 蒋诗艺 +4 位作者 李小军 周正华 杨泽 朱鹏 王杰 《地球物理学报》 SCIE EI CAS 北大核心 2021年第2期582-596,共15页
流体/水分在地球演化过程中具有重要作用,它的存在可以提高地质体的扩散及蠕变,降低岩石/地幔的固液相限,对地质体的地球物理性质、地质灾害诱发、地质构造演化等都有着重要的作用.在板块俯冲区,流体/水分的影响贯穿了整个动力学过程,... 流体/水分在地球演化过程中具有重要作用,它的存在可以提高地质体的扩散及蠕变,降低岩石/地幔的固液相限,对地质体的地球物理性质、地质灾害诱发、地质构造演化等都有着重要的作用.在板块俯冲区,流体/水分的影响贯穿了整个动力学过程,但板块深部脱水还存在着争议,且目前系统地研究水分在整个俯冲中的迁移过程及其地球物理意义的工作还较少.本次研究以我国东北地区为例,借助于地球动力学数值模拟,利用二维岩石化学-热-力学耦合的数值计算程序,建立西太平洋板块向欧亚大陆岩石圈俯冲的数值模拟模型,模拟了板块俯冲整个连续动力学过程中流体/水分的迁移过程.模拟与分析结果表明,板块俯冲过程中水分的迁移过程可分为三个阶段:洋壳水化、板块浅(中)部脱水、板块深部脱水.进一步揭示了洋壳水化过程中水分渗流通道形成、水分渗流以及板块及地幔物质水化反应的机制;解释了板块浅(中)部脱水过程对火山岛弧、弧后盆地及低速异常带形成的作用,以及对~410 km不连续带的影响;模型演化过程中观察到的板块深部脱水现象说明了存在板块深部脱水的可能性,而且板块深部脱水可以较好地解释部分内陆火山以及部分地区地幔柱(岩浆羽)和深源地震的成因.研究展示了板块俯冲过程中水分运移的地球物理意义. 展开更多
关键词 板块俯冲 水分 运移 地球物理意义
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异构云DCN中绿色虚拟算法设计 认领
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作者 黄金凤 《信息技术》 2021年第2期67-72,共6页
为进一步提升异构云数据中心网络(DCN)动态管理的科学性,在总结当前主流研究局限性的基础上构思一种基于全局相对最优化的绿色虚拟算法。算法综合考虑虚拟机迁移过程中可能涉及到的诸多客观因素,通过科学地规划时间门限、主机筛选策略... 为进一步提升异构云数据中心网络(DCN)动态管理的科学性,在总结当前主流研究局限性的基础上构思一种基于全局相对最优化的绿色虚拟算法。算法综合考虑虚拟机迁移过程中可能涉及到的诸多客观因素,通过科学地规划时间门限、主机筛选策略、以及精度比较机制对虚拟机实施高效的迁移。数据考察表明,所部署的算法不仅可快速精确地物色到最适宜的目标主机节点,实施迁移期间的各项指标也均满足绿色要求。且相对于传统算法方案,所消耗的云DCN计算资源更少。 展开更多
关键词 云环境 开销 绿色 虚拟 迁移
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自发性高血压大鼠淋巴微循环功能受损 认领
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作者 王冰 盛有明 +4 位作者 李媛 张晓艳 刘明明 李爱玲 张坚 《微循环学杂志》 2021年第1期1-6,19,共7页
目的:观察自发性高血压大鼠(SHRs)淋巴微循环功能变化。方法:8周龄雄性SHR大鼠(SHR组)和WKY大鼠(WKY组),每组各10只。应用VasTrack测量两组大鼠微淋巴管自律运动;取胸导管分离原代淋巴管内皮细胞(LECs)。应用免疫荧光和Western Blot检测... 目的:观察自发性高血压大鼠(SHRs)淋巴微循环功能变化。方法:8周龄雄性SHR大鼠(SHR组)和WKY大鼠(WKY组),每组各10只。应用VasTrack测量两组大鼠微淋巴管自律运动;取胸导管分离原代淋巴管内皮细胞(LECs)。应用免疫荧光和Western Blot检测LECs血管内皮生长因子受体3(VEGFR3)和淋巴管内皮透明质酸受体-1(LYVE-1)蛋白的表达;应用MTT法、基质胶成管和划痕实验检测两组LECs增殖、成管和迁移能力。结果:SHR组大鼠微淋巴管自律运动功能受损,相对振幅显著降低(P<0.01)。两组LECs LYVE-1、VEGFR3均呈阳性表达,且SHR组LECs VEGFR3蛋白表达水平显著性高于WKY组(P<0.01);SHR组LECs单视野内划痕细胞数显著低于WKY组(P<0.05);SHR组LECs管样结构数量显著增多(P<0.05)。结论:SHR组淋巴微循环功能受损,可能与LECs功能障碍有关。 展开更多
关键词 自发性高血压大鼠 淋巴管内皮细胞 增殖 成管 迁移
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“人才争夺战”背景下人才流动的空间特征及影响因素——以中国“一流大学”毕业生为例 认领
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作者 王一凡 崔璨 +2 位作者 王强 宁越敏 杨振山 《地理研究》 北大核心 2021年第3期743-761,共19页
自2017年以来,“人才争夺战”如火如荼,城市步入以人才为核心要素的高维竞争阶段。作为人才争夺战的主要目标和人力资本的重要承载者,本文聚焦中国“一流大学”毕业生,基于2018年《毕业生就业质量报告》,运用Cartogram地图呈现了毕业生... 自2017年以来,“人才争夺战”如火如荼,城市步入以人才为核心要素的高维竞争阶段。作为人才争夺战的主要目标和人力资本的重要承载者,本文聚焦中国“一流大学”毕业生,基于2018年《毕业生就业质量报告》,运用Cartogram地图呈现了毕业生的空间流动特征,并梳理政府人才政策文件构建了人才政策评价指标体系,运用有向迁移模型剖析毕业生流动的影响因素。结果表明:①就学地存在粘滞性,但区域差异显著,毕业生向一线城市集聚。②城市吸引力水平分异明显,部分城市通过发布人才政策以补充地方经济、舒适性吸引力的不足。③经济维度要素与城市舒适性均能有效吸引毕业生流入,较高房价收入比会引发“挤出效应”。人才政策中,落户政策对毕业生流入起激励作用。 展开更多
关键词 高校毕业生 人才流动 人才争夺战 城市吸引力
基于CiteSpace的国内运动技能迁移研究进程与热点分析 认领
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作者 关朝阳 陈亮 《哈尔滨体育学院学报》 2021年第2期18-24,共7页
为整体了解我国运动技能迁移研究现状与热点趋势,以中国知网1982-2019年间与运动技能迁移研究主题相关的426篇文献为数据支撑,通过知识图谱可视化分析,对国内运动技能迁移研究进行综述。结论:运动技能迁移研究发展至今历经四个阶段,年... 为整体了解我国运动技能迁移研究现状与热点趋势,以中国知网1982-2019年间与运动技能迁移研究主题相关的426篇文献为数据支撑,通过知识图谱可视化分析,对国内运动技能迁移研究进行综述。结论:运动技能迁移研究发展至今历经四个阶段,年发文量总体呈上升趋势,但近年来有所回落;体育类核心期刊发文较少,其发文质量总体不高,解释性、重复性文章较多,交叉性、创新性文章较少;以教学训练为主线,理论研究热点经历了从熟练迁移、正迁移向负迁移、两侧性迁移等方向变化;热点教学项目以体能主导类、隔网对抗类、技能主导类表现难美性项目为主,且运动项目研究热度与时代背景密切相关,这将依旧是国内该领域研究的热门项目类别。 展开更多
关键词 运动技能 迁移 体育教学 CITESPACE
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掺钕钇铝石榴石激光对人滋养层细胞表面抗原2的调控及口腔鳞癌细胞HN6增殖、侵袭和转移的影响 认领
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作者 马浩然 龚爱秀 汤根兄 《实用医学杂志》 CAS 北大核心 2021年第2期175-181,共7页
目的探讨激光对人滋养层细胞表面抗原2(TROP2)调控及对口腔鳞癌细胞HN6增殖、转移和侵袭的影响。方法体外培养口腔鳞癌细胞株HN6,用1064 nm Nd:YAG激光连续照射,检测24、48 h后激光对HN6细胞的增殖、划痕愈合、转移、侵袭的影响。检测Nd... 目的探讨激光对人滋养层细胞表面抗原2(TROP2)调控及对口腔鳞癌细胞HN6增殖、转移和侵袭的影响。方法体外培养口腔鳞癌细胞株HN6,用1064 nm Nd:YAG激光连续照射,检测24、48 h后激光对HN6细胞的增殖、划痕愈合、转移、侵袭的影响。检测Nd:YAG激光对HN6细胞中TROP2表达的影响。TROP2干扰质粒转染至HN6细胞后,检测Nd:YAG激光对HN6细胞增殖和侵袭的影响。结果低剂量和中剂量激光照射时,HN6细胞增殖能力增强,促划痕愈合程度和侵袭能力提高,TROP2表达较高(P<0.05);高剂量激光照射时,HN6细胞增殖受到抑制,划痕愈合明显减慢且侵袭能力受到抑制,TROP2表达较低(P<0.05);干扰TROP2表达后,Nd:YAG激光对HN6细胞增殖与侵袭无影响。结论较低剂量Nd:YAG激光照射促进HN6细胞的增殖、转移和侵袭,高剂量Nd:YAG激光照射抑制HN6细胞的增殖、转移和侵袭。Nd:YAG激光通过调控TROP2的表达影响HN6的增殖及侵袭转移。 展开更多
关键词 ND:YAG激光 TROP2 口腔鳞状细胞 增殖 转移 侵袭
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岩大戟内酯B通过阻断PI3K/Akt/NF-κB通路抑制结肠癌细胞的增殖和转移 认领
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作者 陈林俊 陈文斌 《中国临床药理学与治疗学》 CAS 2021年第3期250-257,共8页
目的:研究岩大戟内酯B(JB)对结肠癌HT-29细胞增殖和转移的抑制作用及其作用机制。方法:用不同浓度JB处理HT-29细胞,采用MTT法检测细胞增殖率;平板克隆实验检测细胞克隆形成率;流式细胞仪检测细胞周期变化;划痕实验分析细胞的迁移能力;Tr... 目的:研究岩大戟内酯B(JB)对结肠癌HT-29细胞增殖和转移的抑制作用及其作用机制。方法:用不同浓度JB处理HT-29细胞,采用MTT法检测细胞增殖率;平板克隆实验检测细胞克隆形成率;流式细胞仪检测细胞周期变化;划痕实验分析细胞的迁移能力;Transwell小室实验研究细胞的侵袭能力;免疫荧光法和Western blotting法检测E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白vimentin、锌指蛋白(Snail)1、Snail2、基质金属蛋白酶(MMP)-2和MMP-9蛋白的表达;Western blotting法检测p-PI3K、PI3K、p-Akt、Akt、NF-κB P65、p-NF-κB P65蛋白表达水平。100μg/L IGF-1组和100μg/L IGF-1+20μmol/L JB组分别处理HT-29细胞,Western blotting法检测PI3K/Akt/NF-κB通路相关蛋白表达变化。结果:JB抑制HT-29细胞增殖作用浓度依赖性,其作用24 h的IC 50为52.68μmol/L;JB呈浓度依赖性地降低HT-29细胞的克隆形成率(P<0.05);与对照组相比,JB处理后的HT-29细胞G0/G1期比例显著增高,S期细胞比例明显下降;JB可显著抑制HT-29细胞的体外迁移、侵袭能力(P<0.05);JB作用后的HT-29细胞E-cadherin蛋白水平升高,vimentin、Snail1、Snail2、N-cadherin、MMP-2、MMP-9、p-PI3K、p-Akt、p-NF-κB P65蛋白表达水平显著降低(P<0.05);IGF-1+JB组p-PI3K、p-Akt、与p-NF-κB P65蛋白的表达水平较IGF-1组显著下降(P<0.05)。结论:JB在体外能抑制HT-29细胞增殖,诱导细胞在G0/G1期阻滞,抑制HT-29迁移和侵袭,调控上皮-间质转化(EMT)及MMPs,其机制可能与阻断PI3K/Akt/NF-κB通路有关。 展开更多
关键词 岩大戟内酯B HT-29细胞 增殖 迁移 上皮-间质转化 PI3K/Akt/NF-κB通路
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miR-140-5p通过调控Wnt1通路对非小细胞肺癌迁移与侵袭能力的影响 认领
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作者 李会荣 钟秀 +2 位作者 王江川 夏峻巍 程德云 《中国免疫学杂志》 CAS 北大核心 2021年第2期186-190,200,共6页
目的:探究miR-140-5p通过Wnt1通路对非小细胞肺癌(NSCLC)细胞迁移、侵袭的影响,为临床对NSCLC的治疗提供理论依据。方法:临床收集人NSCLC组织及癌旁组织20组,RT-PCR检测miR-140-5p与Wnt1基因的表达水平,Spearman相关分析法比较miR-140-5... 目的:探究miR-140-5p通过Wnt1通路对非小细胞肺癌(NSCLC)细胞迁移、侵袭的影响,为临床对NSCLC的治疗提供理论依据。方法:临床收集人NSCLC组织及癌旁组织20组,RT-PCR检测miR-140-5p与Wnt1基因的表达水平,Spearman相关分析法比较miR-140-5p与Wnt1基因表达水平的相关性。将miR-140-5p mimic转染至A549细胞,通过RT-PCR确认过表达。将A549细胞分为对照组、miR-140-5p组、Wnt1组和mimic+Wnt1组,将miR-140-5p mimic和Lv-Wnt1载体分别或共转染至A549细胞,分别通过RT-PCR、Western blot检测Wnt1基因表达水平,并通过细胞划痕实验和Transwell检测各组A549细胞的迁移、侵袭能力。Western blot检测各组细胞中MMP2,MMP9和β-catenin蛋白表达水平。荧光素酶报告基因法验证miR-140-5p与Wnt1基因的靶向关系。结果:与癌旁组织相比,NSCLC组织中miR-140-5p表达量显著降低(P<0.01),且miR-140-5p与Wnt1基因表达水平呈负相关(r=-0.901,P<0.001)。过表达miR-140-5p后,与对照组相比,miR-140-5p组中Wnt1基因在RNA和蛋白质水平的表达量、细胞迁移能力、细胞侵袭数量、MMP2,MMP9、β-catenin蛋白表达量均显著下降(P<0.01),而在Wnt1组中均显著升高(P<0.01)。与Wnt1组相比,mimic+Wnt1组中Wnt1基因在RNA和蛋白质水平表达量、细胞迁移能力、细胞侵袭数量、MMP2、MMP9、β-catenin蛋白表达量均显著下降(P<0.01)。在荧光素酶报告实验中,与WT-Wnt1+miR-140-5p NC组相比,WT-Wnt1+miR-140-5p mimic组中荧光素酶活性显著降低(P<0.01)。结论:miR-140-5p可靶向作用于Wnt1基因,抑制Wnt1通路活性并下调MMP2,MMP9、β-catenin表达,抑制NSCLC细胞迁移与侵袭。 展开更多
关键词 miR-140-5p WNT1 非小细胞肺癌 迁移 侵袭
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