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宠物犬源沙门氏菌和葡萄球菌耐药性及耐药基因检测 预览
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作者 轩慧勇 林亚军 +2 位作者 高超 吾买尔江·牙合甫 夏利宁 《西北农林科技大学学报:自然科学版》 CSCD 北大核心 2019年第3期14-20,共7页
【目的】了解新疆乌鲁木齐市宠物犬源沙门氏菌和葡萄球菌的耐药性及耐药基因携带情况。【方法】从77份宠物犬直肠粪便样品中分离沙门氏菌和葡萄球菌,采用琼脂稀释法对分离的菌株进行药敏试验,通过PCR方法对沙门氏菌6类23种耐药基因和葡... 【目的】了解新疆乌鲁木齐市宠物犬源沙门氏菌和葡萄球菌的耐药性及耐药基因携带情况。【方法】从77份宠物犬直肠粪便样品中分离沙门氏菌和葡萄球菌,采用琼脂稀释法对分离的菌株进行药敏试验,通过PCR方法对沙门氏菌6类23种耐药基因和葡萄球菌5类9种耐药基因进行检测。【结果】从77份宠物犬粪便样品中分离到沙门氏菌36株,葡萄球菌75株。除头孢噻呋、安普霉素、阿米卡星、硫酸庆大霉素和多黏菌素外,宠物犬源沙门氏菌对其他被捡抗菌药物的耐药率高达97.0%以上,多重耐药以8耐为主,tetB、blaTEM、blaOXA、qnrS、oqxA、oqxB、aac(6′)-Ib-cr、ant(3″)-Ia和floR基因检出率在97.0%以上。宠物犬源葡萄球菌对左氧氟沙星(93.5%)、苯唑西林(90.7%)和氟苯尼考(82.9%)耐药率在80.0%以上,多重耐药集中在4耐、6耐和9耐,主要检出的基因为fexA(40.0%,30/75)和femA(18.7%,14/75),其次为tetM(16.0%,12/75)、ermB(14.7%,11/75)和mecA(1.3%,1/75)。【结论】宠物犬源沙门氏菌和葡萄球菌耐药情况严重,耐药基因携带率高,使宠物犬的细菌性疾病治疗难度加大,应加强耐药菌的监测,降低宠物犬源耐药菌转移给人的潜在风险。 展开更多
关键词 宠物犬 沙门氏菌 葡萄球菌 耐药性 耐药基因检测
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华北地区部分省(市)奶牛乳房炎乳中链球菌耐药表型和耐药基因研究
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作者 田晓英 甄天元 +4 位作者 于忠娜 孙萍 王军 韩荣伟 王世清 《黑龙江畜牧兽医》 CAS 北大核心 2019年第1期1-7,共7页
为了解华北地区4个省(市)奶牛乳房炎乳中链球菌的感染情况及对常用抗生素的耐药性,试验采用EN液体培养基、5%绵羊鲜血琼脂培养基、触酶试验、6.5%NaCl试验、七叶苷试验i马尿酸钠试验和CAMP试验对2016年华北地区240批次乳房炎奶牛乳样进... 为了解华北地区4个省(市)奶牛乳房炎乳中链球菌的感染情况及对常用抗生素的耐药性,试验采用EN液体培养基、5%绵羊鲜血琼脂培养基、触酶试验、6.5%NaCl试验、七叶苷试验i马尿酸钠试验和CAMP试验对2016年华北地区240批次乳房炎奶牛乳样进行链球菌的分离鉴定和分型;采用纸片扩散法对2016年华北地区分离出的链球菌进行药敏试验,对华北地区上下半年及4个省(市)链球菌的药敏情况进行对比;并通过PCR扩增技术研究链球菌的耐药基因,计算链球菌表型耐药率与所携带相关耐药基因的阳性率,比较两者的相符率。结果表明:共分离得到致病性链球菌28株,总分离率为11.67%,其中无乳链球菌7株、停乳链球菌5株和乳房链球菌16株;链球菌分离株对苯唑西林和青霉素G表现出完全耐药;对四环素类药物耐药性较高,强力霉素和四环素的耐药率分别为85.71%和82.14%;对氯霉素类药物高度敏感,敏感率为92.86%;对喹诺酮类和氨基糖苷类药物耐药性较低,平均耐药率分别为8.93%和10.20%;除磺胺类药物外,其他类药物耐药率下半年均低于上半年;在4个省(市)中,天津市链球菌分离株对各类抗生素的平均耐药率高于其他省(市);经PCR扩增检测,耐药基因阳性率在7.14%~26.79%之间,整体检出率较低,耐药基因与耐药表型相符率在0~100%之间,其中氯霉素相符率为0,并不能完全拟合。说明2016年我国华北地区链球菌总检出率较低,乳房链球菌为主要致病菌,链球菌分离株表现出不同程度的耐药性,且多为多重耐药,耐药基因与耐药表型的相符率较低。 展开更多
关键词 奶牛乳房炎 链球菌 分型 抗生素 耐药性 耐药基因
金属铝管影响下磁共振耦合线圈特性参数变化 预览
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作者 王乃健 李长生 +1 位作者 曹娟 缪东辉 《火力与指挥控制》 CSCD 北大核心 2019年第1期23-28,33共7页
为了探究金属物体对磁共振耦合线圈自身特性参数的影响规律,针对现有理论模型不能完全描述金属铝管对线圈特性参数影响规律的问题,构建了金属铝管影响下的磁共振耦合单线圈修正模型,对应开展了仿真与实验研究,并给出了涡电流回路映射电... 为了探究金属物体对磁共振耦合线圈自身特性参数的影响规律,针对现有理论模型不能完全描述金属铝管对线圈特性参数影响规律的问题,构建了金属铝管影响下的磁共振耦合单线圈修正模型,对应开展了仿真与实验研究,并给出了涡电流回路映射电阻R’E和电阻修正量RC具体表达式。研究规律表明:线圈电感参数变化量△L随间隙的增大而减小;线圈电阻参数变化量△R的变化趋势与间隙相关且存在变化趋势分界点,即当铝管与线圈间隙小于10 mm时,线圈电阻参数数值相对放置于空气中是增大的;当铝管与线圈间隙达到15 mm时,线圈电阻参数数值相对放置于空气中是减小的。 展开更多
关键词 金属铝管 磁共振耦合线圈 电阻 电感 电阻修正量
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猪链球菌对大环内酯类抗生素的耐药性研究 预览
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作者 蔡田 罗行炜 +5 位作者 徐引第 魏单单 贺丹丹 刘建华 苑丽 胡功政 《河南农业大学学报》 CAS CSCD 北大核心 2019年第1期73-81,共9页
为测定猪链球菌对大环内酯类抗生素的耐药性和相关耐药基因分布特点,分析分离株对大环内酯类抗生素耐药的分子机制。从河南等7省份不同猪场采集来的疑似猪链球菌患病猪的脑脊液、关节液、肝等部位分离、纯化疑似菌落,采用镜检、16SrRNA... 为测定猪链球菌对大环内酯类抗生素的耐药性和相关耐药基因分布特点,分析分离株对大环内酯类抗生素耐药的分子机制。从河南等7省份不同猪场采集来的疑似猪链球菌患病猪的脑脊液、关节液、肝等部位分离、纯化疑似菌落,采用镜检、16SrRNA基因方法鉴定猪链球菌。采用肉汤微量稀释法检测分离猪链球菌对大环内酯类抗生素红霉素、泰乐菌素、替米考星的耐药情况,采用PCR方法检测相关耐药基因(包括编码核糖体甲基化酶的erm基因ermA、ermB、ermC、ermTR和介导外排泵机制的mefA、mefE及msrD基因)分布情况。最终从573份病料分离鉴定出猪链球菌165株,分离率为28.8%。药敏试验结果显示:猪链球菌对大环内酯类抗生素耐药率高达98.1%,对红霉素、泰乐菌素、替米考星耐药率分别达96.9%、95.1%、94.5%,有153株菌株对3种抗生素同时耐药。耐药基因检测发现大环内酯类耐药基因ermA、ermB、ermC、ermTR、mefA、mefE、msrD检出率分别为5.4%、81.8%、13.3%、6.6%、11.5%、7.2%、5.4%,其中ermB检出率最高。除发现有93株菌株仅携带1个耐药基因外,分别有30、17、2、1个菌株分别携带2、3、4、6个大环内酯类耐药基因。在检出耐药基因的菌株中有82.5%(118/143)的菌株仅携带erm基因,有13.9%(20/143)的菌株同时携带erm以及mef、msr基因,另有3%(5/143)的菌株仅携mef、msr基因。猪链球菌对大环内酯类药物广泛耐药,大环内酯类耐药基因在猪链球菌中广泛分布,细菌的耐药性和菌株耐药基因携带情况基本呈正相关。以ermB介导的核糖体23SrRNA甲基化耐药机制占主导地位,由mefA、mefE、msrD介导的外排泵机制亦存在于分离菌株中。 展开更多
关键词 猪链球菌 大环内酯类 耐药性 耐药机制
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对苦难的反抗与超越--论《平凡的世界》乡土化与城市化影响下的人物抗争
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作者 余青 王颖怡 曹慧 《新余学院学报》 2019年第1期108-110,共3页
《平凡的世界》讲述了孙少安和孙少平两兄弟的人生经历和喜怒哀乐,深刻诠释了"苦难"的精神实质。作为亲兄弟的少平和少安,在接踵而至的磨难面前做出了不同反应:受不同思想环境的熏陶,源于乡土化给少安带来的是对现实的清醒认... 《平凡的世界》讲述了孙少安和孙少平两兄弟的人生经历和喜怒哀乐,深刻诠释了"苦难"的精神实质。作为亲兄弟的少平和少安,在接踵而至的磨难面前做出了不同反应:受不同思想环境的熏陶,源于乡土化给少安带来的是对现实的清醒认识,使他成为务实派,而城市化给少平带来的是对外面世界的无限向往,使之成为理想主义者。无论是务实派还是理想主义者,他们最终都凭借自己的努力实现了对苦难的反抗与超越。 展开更多
关键词 《平凡的世界》 苦难 反抗
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十二指肠巨大间质瘤1例并文献复习 预览
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作者 邵胜利 冯永东 《世界最新医学信息文摘(电子版)》 2019年第16期252-253,共2页
目的研究十二指肠巨大间质瘤临床特点、诊疗现状及最新进展。方法利用1例临床病例及查阅文献的方式研究目前对于十二指肠间质瘤的治疗方式及相应不足。结果十二指肠间质瘤是相对罕见的,目前仍缺少完善的诊疗指南。结论十二指肠有其特殊... 目的研究十二指肠巨大间质瘤临床特点、诊疗现状及最新进展。方法利用1例临床病例及查阅文献的方式研究目前对于十二指肠间质瘤的治疗方式及相应不足。结果十二指肠间质瘤是相对罕见的,目前仍缺少完善的诊疗指南。结论十二指肠有其特殊的解剖关系手术方式因肿瘤的大小、部位、与周围解剖部位的关系不同而大有不同,靶向药物耐药问题仍待解决。 展开更多
关键词 间质瘤 十二指肠 巨大包块 手术方式 耐药
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葡萄种质资源对晚霜冻害的抗性表现
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作者 张剑侠 《果树学报》 CAS CSCD 北大核心 2019年第2期137-142,共6页
【目的】了解葡萄种质资源的抗晚霜表现,为育种利用提供参考依据。【方法】晚霜发生后3 d,对中国野生葡萄17种或变种的56个株系、美国野生葡萄6种的8个株系及17个栽培品种(系)共81份葡萄种质资源抗晚霜冻害表现进行自然鉴定。【结果】... 【目的】了解葡萄种质资源的抗晚霜表现,为育种利用提供参考依据。【方法】晚霜发生后3 d,对中国野生葡萄17种或变种的56个株系、美国野生葡萄6种的8个株系及17个栽培品种(系)共81份葡萄种质资源抗晚霜冻害表现进行自然鉴定。【结果】中国野生葡萄中,毛葡萄、桑叶葡萄、瘤枝葡萄、华东葡萄表现为避霜;燕山葡萄、麦黄葡萄、麦黄复叶葡萄高抗;蘡薁葡萄高抗或抗;陕西葡萄、小复叶葡萄、菱叶葡萄抗;山葡萄、秋葡萄、复叶葡萄、刺葡萄、秦岭葡萄种内存在抗性差异,既有高抗或抗的株系,也有不抗或极不抗的株系;裂叶刺葡萄’宁强-6’极不抗。美国野生葡萄中,冬葡萄为避霜;美洲葡萄、甜冬葡萄高抗;河岸葡萄、峡谷葡萄和沙地葡萄抗。栽培品种中,欧亚种品种’无核白’高抗’,早玫瑰’’早金香’’绯红’抗’,无核紫’’新郁’’京秀’不抗;欧美杂种’巨峰’高抗’,藤稔’’早生高墨’’京优’’木星’和’海王星’抗;欧山杂种’00-1-10’为避霜’,00-1-5’高抗’,北醇’和’左优红’不抗。【结论】中国野生葡萄、栽培品种抗晚霜表现具丰富多样性,美国野生葡萄表现出较强的抗性。葡萄种质资源对晚霜抗性表现与冬季抗寒性表现不完全一致。 展开更多
关键词 葡萄 种质资源 晚霜 冻害 抗性
2014—2017年解放军总医院海南分院真菌分布及耐药性分析
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作者 沈绍清 于帅 +1 位作者 陈怡君 蔡芸 《现代药物与临床》 CAS 2019年第1期252-256,共5页
目的了解2014—2017年解放军总医院海南分院真菌的分布和耐药特点,为促进抗真菌药的合理应用、有效减缓真菌耐药提供参考。方法提取解放军总医院海南分院2014年1月—2017年12月临床真菌分离和药敏相关数据,分析真菌的分布特征及其对常... 目的了解2014—2017年解放军总医院海南分院真菌的分布和耐药特点,为促进抗真菌药的合理应用、有效减缓真菌耐药提供参考。方法提取解放军总医院海南分院2014年1月—2017年12月临床真菌分离和药敏相关数据,分析真菌的分布特征及其对常用抗真菌药的耐药率和变化趋势。结果共纳入1048份阳性真菌样本,呼吸道标本占41.89%,60岁以上患者(61.45%)和重症医学科(25.48%)分布比例最高;共分离出1 329株真菌,白色念珠菌、念珠菌属、热带念珠菌分别占24.53%、15.80%、13.69%。各种念珠菌对两性霉素B和5-氟胞嘧啶的敏感率基本保持在90%以上;热带念珠菌对伊曲康唑耐药率最高,达10%~20%,对氟康唑和伏立康唑的耐药率亦高于两性霉素B和5-氟胞嘧啶;白色念珠菌对伊曲康唑和伏立康唑的耐药率呈逐年快速上升趋势。结论白色念珠菌和热带念珠菌感染应慎用唑类抗真菌药;两性霉素B和5-氟胞嘧啶是念珠菌感染的有效选择。 展开更多
关键词 真菌 热带念珠菌 抗真菌药 耐药性 感染
基于microRNA测序分析miRNA在刺葡萄抗白腐病中的作用
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作者 张颖 樊秀彩 +2 位作者 姜建福 李民 刘崇怀 《果树学报》 CAS CSCD 北大核心 2019年第2期143-152,共10页
【目的】在前期的种质资源评价过程中,发掘一株中国野生刺葡萄0943,该株系高抗葡萄白腐病。基于mi-croRNA(miRNA)在植物抗病中的重要作用,拟从miRNA水平探讨刺葡萄在受到病原菌侵染后的表达调控机制。【方法】以抗病的中国野生刺葡萄为... 【目的】在前期的种质资源评价过程中,发掘一株中国野生刺葡萄0943,该株系高抗葡萄白腐病。基于mi-croRNA(miRNA)在植物抗病中的重要作用,拟从miRNA水平探讨刺葡萄在受到病原菌侵染后的表达调控机制。【方法】以抗病的中国野生刺葡萄为试材,对比感病的欧亚种’美人指’,分别以病原菌诱导,在0 hpi(hours post inoculation)和病菌诱导后的12 hpi、36 hpi处理后采样,进行二代测序,并对数据进行KEGG及表达量的差异分析。【结果】对比感病葡萄’美人指’,分析了抗病刺葡萄在基础代谢和抗病途径中的差异,结合miRNA的表达量,获得了150个表达量发生变化的miRNA,其中44个miRNA的表达在刺葡萄和’美人指’之间存在差异。5个miRNA在刺葡萄中特异表达,但是在’美人指’中不表达,实施定量验证了这一结果。靶基因预测显示,其靶基因包括与抗病的紧密相关的WRKY、SPL、EFR等转录因子,还包括与抗病直接相关的LRR类的抗病基因。【结论】筛选出5个在刺葡萄上特异表达候选miRNA(miR172a、miR172b、miR845a、novel81和miR159a),可作为刺葡萄抗白腐病研究的目标。 展开更多
关键词 葡萄白腐病 MICRORNA 抗病 测序
硅肥对水稻增产提质抗病虫的影响研究进展 预览
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作者 于广星 宫殿凯 +4 位作者 代贵金 刘宪平 陈盈 李海波 侯守贵 《中国稻米》 2019年第1期21-22,25共3页
在“两减一增”目标下的农业生产要打破常规的惯性思维,科学布局;在减少使用化肥农药的情况下,笔者认为可以通过合理施用硅肥来实现稳产增产。本文综合阐述了硅肥施用对水稻增产提质,提高水稻对病虫害的抗性以及对重金属镉吸收转运的影... 在“两减一增”目标下的农业生产要打破常规的惯性思维,科学布局;在减少使用化肥农药的情况下,笔者认为可以通过合理施用硅肥来实现稳产增产。本文综合阐述了硅肥施用对水稻增产提质,提高水稻对病虫害的抗性以及对重金属镉吸收转运的影响,使广大稻农意识到使用硅肥的重要性。 展开更多
关键词 水稻 硅肥 产量 品质 抗性
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Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa(Jacq.) Raf.(Cleomaceae)
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作者 Francisca Samara Muniz dos Santos JoséWeverton Almeida Bezerra +8 位作者 Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Ant?nio Ivanildo Pinho Henrique Douglas Melo Coutinho JoséEdilson Gon?alves dos Santos 《亚太热带生物医学杂志:英文版》 CAS 2019年第1期12-17,共6页
Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa(T. spinosa) as well as to determine and quantify some of its polyphenols by high... Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa(T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection(HPLC-DAD). Methods: The bacterial Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa strains, grown in Heart Agar Infusion, were tested. The drugs gentamicin, norfloxacin and imipenem were used to evaluate the modulating or antagonistic capacity of the T. spinosa extracts. The extract was analysed by HPLC-DAD to determine the main phenolic compounds. For the cell viability tests, individual heads of the Nauphoeta cinerea arthropod model were removed, homogenized in Trifluoromethyl ketone and centrifuged afterwards. Subsequently, 20 μL of NaN O2 were added to the biological material, except in the control group, to evaluate the protection capacity of the extracts. The homogenate of the insect heads was incubated for 2 h in tubes containing tetrazolium bromide. Results: HPLC-DAD demonstrated that the ethanolic extract of T. spinosa presented caffeic acid as the major compound. The ethanolic extract also showed neuroprotective effects at concentrations ≥ 10 μg/mL, while aqueous extract was shown to have a protective effect only at the concentration of 100 μg/mL. The aqueous extract demonstrated a clinically relevant antibacterial activity against the Staphylococcus aureus multidrug resistant strain-MDR, with MIC 512 μg/m L. However, when the extracts were associated with gentamicin and imipenem, a synergism was detected against Staphylococcus aureus and Escherichia coli MDR strains. Conclusions: Although it does not present an antibacterial action, the extracts of T. spinosa can be used in the pharmaceutical industries since its extracts show modulating action of drugs. Besides, these natural products have neuroprotective capacity. 展开更多
关键词 Tarenaya spinosa CLEOME spinose Nauphoeta CINEREA Synergism Antibiotic resistance
Micropropagation of Pinus densiflora and the evaluation of nematode resistance of regenerated microshoots in vitro 预览
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作者 Lihua Zhu Xiaofang Chu +2 位作者 Tingyu Sun Jianren Ye Xiaoqin Wu 《林业研究:英文版》 CAS CSCD 2019年第2期519-528,共10页
To accelerate the breeding and selection of Pinus densiflora Siebold and Zucc.resistance to pine wilt disease,a micropropagation system was established and nematode resistance evaluated in vitro.Cotyledon-hypocotyl ex... To accelerate the breeding and selection of Pinus densiflora Siebold and Zucc.resistance to pine wilt disease,a micropropagation system was established and nematode resistance evaluated in vitro.Cotyledon-hypocotyl explants from 28-day-old seedlings were first cultured on Gresshoff and Doy medium supplemented with 4.0 mg L^-1 6-benzyladenine and 0.2 mg L^-1 a-naphthaleneacetic acid(NAA)to stimulate the formation of buds.Induced buds were subsequently subcultured on Gupta and Durzan medium supplemented with 0.1%(w/v)activated charcoal for elongation.Stem sections derived from shoots were used as explants for the further multiplication.Roots were formed from shoots transferred to woody plant medium containing 0.2 mg L^-1 NAA for 4 weeks.The nematode resistance test showed that symptoms in micropropagated shoots after infection with pine wood nematode(PWN)were similar to those in plants infected in the field.The wilting rate varied from 20 to 100%among different clones 18 days after inoculation.The most susceptible clone was Clone 6-4 with a 100%wilting rate,while Clone 8-4 showed a relatively high resistance with a 20%wilting rate.The number of nematodes recovered from Clone 8-4 shoots was significantly lower(P=0.05)than from Clones 5-10 and 16-4.This work contributes to the breeding of PWN resistance in P.densiflora. 展开更多
关键词 PINUS densiflora MICROPROPAGATION SHOOT PROLIFERATION BURSAPHELENCHUS xylophilus NEMATODE resistance
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An integrated approach to assess the ecological and chemical status of lakes with HOD/AHOD:a case study of two lakes 预览
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作者 Serdar GONCU Erdem ALBEK 《海洋湖沼学报(英文)》 SCIE CAS CSCD 2019年第1期146-159,共14页
The thermal stratification of the lakes impedes the transfer of atmospheric oxygen into the lower layers of the lake.In lakes which are affected by diverse anthropogenic infl uences,the increasing organic matter amoun... The thermal stratification of the lakes impedes the transfer of atmospheric oxygen into the lower layers of the lake.In lakes which are affected by diverse anthropogenic infl uences,the increasing organic matter amounts lead to a sharp decrease in hypolimnetic oxygen amounts,aided by thermal stratification,and anaerobic conditions arise.The determination of hypolimnetic oxygen demand(HOD)and areal hypolimnetic oxygen demand(AHOD)and their monitoring represent an integrated approach to investigate the oxygenation of lakes,the nutrient conditions,and the physicochemical dynamics.In this study,two lakes differing by size and affected by different anthropogenic sources,are investigated in this respect.At first,bathymetric studies were conducted to determine the depth,surface area,and volume relationships.Then,based on monitoring studies conducted in 2013 and 2014,the thermal stratification dynamics and layer properties were established using the relative thermal resistance to mixing(RTRM)index based on temperature and density profiles.Following this,the oxygen depletion rates were determined by oxygen and temperature profiling in the hypolimnion.For the years of 2013 and 2014,the AHOD values for the Borabey Pond which is far from anthropogenic infl uences,were found to be 0.848 and 0.569 g O 2/(m·d),respectively.The AHOD values for the Porsuk Reservoir which was overburdened for years by industrial and domestic pollution were found to be 4.263 and 5.099 g O 2/(m·d),larger than its counterpart by almost sevenfold.The HOD and AHOD monitoring can be considered to a valuable tool for assessing the ecological and chemical status of lakes within Annex 5 of the Water Framework Directive and as an integrated approach to assess and monitor the status of lakes. 展开更多
关键词 hypolimnetic OXYGEN demand(HOD) areal hypolimnetic OXYGEN demand(AHOD) THERMAL stratification relative THERMAL resistance to mixing(RTRM) Borabey POND Porsuk Dam
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Coconut Oil Reduces Visceral Adipocyte Size and Improves the Metabolic Profile of Rats Fed a High-Carbohydrate Diet 预览
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作者 Valéria Schoffen Romao-Carrascoza Rosangela Fernandes Garcia +4 位作者 Lorena Lima Garo Maria Montserrat Diaz Pedrosa Nilton de Almeida Brito Clairce Luzia Salgueiro-Pagadigorria Márcia do Nascimento Brito 《药剂与药理学:英文版》 2019年第3期98-109,共12页
This work aimed to evaluate the effects of the food supplementation with coconut oil(CO)on biometric and metabolic parameters in an animal model of obesity.Wistar rats fed a high-carbohydrate diet(HCD rats)develop met... This work aimed to evaluate the effects of the food supplementation with coconut oil(CO)on biometric and metabolic parameters in an animal model of obesity.Wistar rats fed a high-carbohydrate diet(HCD rats)develop metabolic disorders very similar to those found in human metabolic syndrome,including increased adiposity,insulin resistance(IR),non-alcoholic fatty liver disease(NAFLD)and dyslipidemia.Although HCD rats had normal fasting glycemia,they developed IR as revealed by Homa-IR index and,after glucose overload,exhibited higher peaks of insulinemia and glycemia.These alterations were accompanied by a higher formation of advanced glycated end products(AGEs).The supplementation with CO improved IR,reduced AGEs formation and reverted NAFLD.These effects could be due to CO action of reducing visceral adipocyte size and take place without undesirable collateral effects on lipid profile,placing CO as an adjuvant in the treatment of the metabolic syndrome. 展开更多
关键词 ADIPOCYTE HYPERTROPHY METABOLIC syndrome insulin resistance NAFLD COCONUT oil
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Preparation and Corrosion Resistance of Anodic Oxidation Coatings on 2198 and 5A90 Al-Li Alloys
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作者 王赫男 LI Xinming +2 位作者 HE Linbo CUI Baolei LIU Chunzhong 《武汉理工大学学报:材料科学英文版》 SCIE EI CAS 2019年第1期132-137,共6页
2198 and 5A90 Al-Li alloys were anodized with a constant DC potential in 18%H2SO4solution(Solu.A) and the mixture solution of 18%H2SO4+5%C2H2O4(Solu.B) at room temperature. 12 and 11 V was optimized as the applied oxi... 2198 and 5A90 Al-Li alloys were anodized with a constant DC potential in 18%H2SO4solution(Solu.A) and the mixture solution of 18%H2SO4+5%C2H2O4(Solu.B) at room temperature. 12 and 11 V was optimized as the applied oxidation potential for 2198 and 5A90 alloys, respectively. Cross-sectional morphology, surface morphology and elements distribution of anodic oxidation coatings were observed by scanning electron microscope equipped with energy dispersive X-ray analysis(SEM/EDX). Corrosion resistance was tested by potentiodynamic polarization plot in 3.5%NaCl solution. The results showed that the thicknesses of coatings obtained at the selected potential in Solu.A and Solu.B were about 50 μm/110 μm for 2198 alloy and 80 μm/110 μm for 5A90 alloy. In both solutions, anodic oxidation coatings of 2198 alloy were primarily composed of Al oxides;those of 5A90 alloy were mainly consisted of Al oxides and a small amount of Mg oxides. The results of potentiodynamic polarization showed that anodic oxidation coatings of 2198 and 5A90 Al-Li alloys had better corrosion resistances than that of untreated alloys. 展开更多
关键词 AL-LI alloy the MIXTURE solution anodic OXIDATION COATING corrosion resistance thickness
Patrinia scabiosaefolia Inhibits Growth of 5-FU-Resistant Colorectal Carcinoma Cells via Induction of Apoptosis and Suppression of AKT Pathway
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作者 HUANG Si-zhou LIU Wang-yu +3 位作者 HUANG Yue SHEN A-ling LIU Li-ya PENG Jun 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第2期116-121,共6页
Objective: To investigate the effects of ethanol extract of Patrinia scabiosaefolia(EEPS) on chemo-resistance of colorectal cancer cells(CRC) and explore the possible molecular mechanisms. Methods: 5-fluorouracil(5-FU... Objective: To investigate the effects of ethanol extract of Patrinia scabiosaefolia(EEPS) on chemo-resistance of colorectal cancer cells(CRC) and explore the possible molecular mechanisms. Methods: 5-fluorouracil(5-FU)-resistant human colorectal carcinoma cell line(HCT-8/5-FU) and its parental cells HCT-8 were treated with EEPS(0, 0.25, 0.50, 1 or 2 mg/mL), or 5-FU(0, 100, 200, 400, 800 or 1600 μmol/L). The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to evaluate the cell viability. Cell density was observed by phase-contrast microscope, cell counting and colony formation assay were used to determine the cell proliferation of HCT-8/5-FU cells treated with 0, 0.5, 1 or 2 mg/mL EEPS. Cell apoptosis was determined by Hoechst staining. Western-blot was performed to detect the phosphorylation of AKT as well as the protein expression level of B-cell CLL/lymphoma 2(Bcl-2) and Bcl-2-associated X protein(Bax). Results: Compared with HCT-8 cells, MTT assay results indicated that HCT-8/5-FU cells were resistant to 5-FU treatment(P<0.05), and sensitive to EEPS treatment(P>0.05). Moreover, compared with untreated HCT-8/5-FU cells, 1 and 2 mg/mL of EEPS treatment significantly reduced cell density, cell number, inhibited cell survival(P<0.05), and induced apoptosis in HCT-8/5-FU cells. Furthermore, 1 and 2 mg/mL of EEPS significantly decreased the phosphorylation level of p-AKT and Bcl-2 protein expression, and increased the expression of Bax protein(P<0.05). Conclusion: EEPS is a promising therapeutic agent that may overcome chemo-resistance in cancer cells, likely through suppression of the AKT pathway and promotion of cancer cell apoptosis. 展开更多
关键词 AKT PATHWAY COLORECTAL cancer 5-FLUOROURACIL resistance Patrinia scabiosaefolia Chinese medicine
Strength and Wear Behavior of Mg Alloy AE42 Reinforced with Carbon Short Fibers
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作者 Sabbah Ataya Naser A.Alsaleh Mohamed M.El-Sayed Seleman 《金属学报:英文版》 SCIE EI CAS CSCD 2019年第1期31-40,共10页
In addition to the advantage of the lightweight of magnesium alloys, magnesium composites have moderate strength and elastic modulus. The proposed application of magnesium composites as diesel truck pistons makes it n... In addition to the advantage of the lightweight of magnesium alloys, magnesium composites have moderate strength and elastic modulus. The proposed application of magnesium composites as diesel truck pistons makes it necessary to assess their wear performance. Little research data have been discussed on wear behavior of Mg alloy AE42 matrix and its composites. Thus, this paper reports wear behavior of magnesium alloy AE42(Mg–Al–Mn—RE;rare earth) and its composite AE42-C, which contains 23 vol% of randomly oriented carbon short fibers. Materials characterization, including density measurements, hardness testing, microstructures investigation, and compression testing at temperatures of 25, 150,and 300 °C, were conducted. Wear tests were performed under various loads and sliding distances. Wear mechanisms were also proposed based on the examination of worn surfaces using optical microscopy and scanning electron microscopy equipped with EDX(energy-dispersive X-ray spectrometry) analysis system. The hardness of AE42-23 vol% C composite is twice the hardness of the Mg matrix alloy AE42. Significant improvements to yield stress and compressive strength at temperatures of 25, 150, and 300 °C of the composite versus the AE42 alloy are achieved. Wear resistance of the composite is improved considerably versus that of the Mg alloy AE42 at the various sliding distances. Smearing of graphite on the worn surface produces a lubricating film that delays change from mild to severe wear of the composite, especially at high loads. EDX analysis of the worn surface shows oxidation of the matrix alloy at higher wear loads, and this mechanism decreases in the presence of carbon fibers under the same loads. Abrasive wear, oxidation, and plastic deformation are the dominant wear mechanisms for the alloy matrix AE42, whereas mainly abrasive wear is the wear mechanism of AE42-23 vol% C composite under the proposed testing conditions. 展开更多
关键词 Magnesium composites CARBON short fibers COMPRESSIVE STRENGTH WEAR resistance WEAR mechanisms
不同菌属的氟苯尼考耐药特点及floR基因的序列分析 预览
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作者 吴方达 《中国农学通报》 2019年第2期122-126,共5页
为了解福建宁德地区猪源细菌对氟苯尼考耐药性及耐药基因floR的遗传特性。本研究从宁德地区6个猪场分别采集了50份仔猪粪便样品。从中分离得到氟苯尼考耐药大肠杆菌17株、沙门氏菌2株、肺炎克雷伯菌5株和鲍曼不动杆菌8株。并对其耐药基... 为了解福建宁德地区猪源细菌对氟苯尼考耐药性及耐药基因floR的遗传特性。本研究从宁德地区6个猪场分别采集了50份仔猪粪便样品。从中分离得到氟苯尼考耐药大肠杆菌17株、沙门氏菌2株、肺炎克雷伯菌5株和鲍曼不动杆菌8株。并对其耐药基因floR进行同源性分析。结果显示:宁德地区猪源大肠杆菌、沙门氏菌、肺炎克雷伯菌和鲍曼不动杆菌对氟苯尼考的耐药检出率分别为34%、4%、10%和16%。且所有的分离细菌都呈现了多重耐药性。同源性分析发现不同细菌间的耐药基因floR存在高度同源性。这些结果表明氟苯尼考耐药基因floR广泛存在于不同细菌间,且可在不同细菌间进行水平传播。该研究为氟苯尼考耐药基因floR的广泛传播奠定了一定的理论基础,对临床合理用药提供了依据。 展开更多
关键词 氟苯尼考 耐药性 floR基因 猪源细菌
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冠心病监护病房住院患者感染病原菌种类及耐药性分析 预览
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作者 邱浩 王延政 刘妍妍 《世界最新医学信息文摘(电子版)》 2019年第6期28-29,31共3页
目的探讨冠心病监护病房(CCU)住院患者感染的病原菌种类及耐药情况,为抗菌药物临床正确应用提供参考。方法采用VITEK-32全自动微生物分析仪对CCU送检的患者标本进行病原学检测和药敏试验。结果 356份标本中共检出163株病原菌,分离率为45... 目的探讨冠心病监护病房(CCU)住院患者感染的病原菌种类及耐药情况,为抗菌药物临床正确应用提供参考。方法采用VITEK-32全自动微生物分析仪对CCU送检的患者标本进行病原学检测和药敏试验。结果 356份标本中共检出163株病原菌,分离率为45.8%;其中有46株革兰阳性球菌(占28.22%);117株革兰阴性杆菌(占71.78%)。肺炎链球菌(13.5%),金黄色葡萄球菌(9.82%),凝固酶阴性葡萄球菌(4.9%)是革兰阳性球菌中常见的致病菌;鲍曼不动杆菌(26.38%),铜绿假单胞菌(19.02%),肺炎克雷伯菌(11.66%),大肠埃希菌(8.59%),流感嗜血杆菌(6.13%)为革兰阴性菌中常见的致病菌。不同的病原菌有其自身的耐药特点,革兰阳性球菌中的肺炎链球菌、金黄色葡萄球菌和凝固酶阴性葡萄球菌耐药率较高,对青霉素、红霉素和克林霉素的耐药率均达60%以上;肺炎链球菌对苯唑西林、美罗培南、亚胺培南、氧氟沙星、左氧氟沙星、莫西沙星有较低的耐药率(小于20%);目前没有发现耐万古霉素和耐利奈唑胺菌株。鲍曼不动杆菌对氨苄西林和头孢替坦的耐药率分别高达95.35%和93.02%;铜绿假单胞菌对氨苄西林舒巴坦和复方新诺明的耐药率分别为100.00%和96.77%。革兰阴性杆菌中耐药率较低的为亚胺培南、阿米卡星和含酶抑制剂类抗菌药物如哌拉西林他唑巴坦、头孢哌酮舒巴坦。结论由于长期广泛应用抗菌药物,导致常见病原菌对多种抗菌药物的耐药性发生变化,临床医生在使用抗菌药物前应先进行细菌培养,根据药敏试验结果,正确合理应用抗菌药物。 展开更多
关键词 冠心病监护病房 病原菌 耐药性
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ALK抑制剂治疗非小细胞肺癌的研究进展
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作者 谷瑞雪 邢影 蔡莉 《中国肿瘤》 CAS CSCD 北大核心 2019年第2期126-131,共6页
肺癌是最常见的恶性肿瘤之一,且为全世界范围内肿瘤相关性死亡的首要原因,其中非小细胞肺癌(non-small cell lung cancer,NSCLC)约占肺癌总数的85%,预后较差。间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)融合基因为促进肺癌进展... 肺癌是最常见的恶性肿瘤之一,且为全世界范围内肿瘤相关性死亡的首要原因,其中非小细胞肺癌(non-small cell lung cancer,NSCLC)约占肺癌总数的85%,预后较差。间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)融合基因为促进肺癌进展的一个驱动基因,约2%~8%的NSCLC患者具有ALK融合基因,其对NSCLC的发生和发展至关重要。近年来靶向ALK融合基因已成为NSCLC治疗的前沿手段,第一、二、三代ALK酪氨酸激酶抑制剂(ALK-tyrosine kinase inhibitors,ALK-TKIs)治疗ALK阳性的NSCLC显示出较好的疗效,但仅在一定时间内对特定人群有效。因此规范应用ALK-TKIs、探讨耐药机制及应对不良反应等是临床面临的难题。本文关于这些问题的研究进展作一综述。 展开更多
关键词 肺肿瘤 ALK抑制剂 耐药
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