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Impacts of Sletr1-1 and Sletr1-2 mutations on the hybrid seed quality of tomatoes 预览
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作者 Syariful Mubarok Hiroshi Ezura +4 位作者 Anas Kusumiyati Neni Rostini Erni Suminar Gungun Wiguna 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第5期1170-1176,共7页
Shelf life is an important breeding trait in tomato, especially for the tomato production in subtropical and tropical regions. Previously we have isolated and characterized ethylene receptor mutants, Sletr1-1 and Slet... Shelf life is an important breeding trait in tomato, especially for the tomato production in subtropical and tropical regions. Previously we have isolated and characterized ethylene receptor mutants, Sletr1-1 and Sletr1-2 from mutant population based on Micro-Tom cultivar. Sletr1-1 showed insensitivity to ethylene while Sletr1-2 showed reduced sensitivity to ethylene. We also have demonstrated that the traits are useful for extending fruit shelf life of the hybrid tomato cultivars. For commercializing the hybrid cultivars, the seed quality is another important trait. In this study, we evaluated the effects of the Sletr1-1 and Sletr1-2 mutations on the seed quality characteristics of F1 hybrid lines generated by crossing Sletr1-1 and Sletr1-2 with three commercial tomato cultivars, Intan, Mutiara and Ratna. Sletr1-1 mutation conferred insensitivity to ethylene in the F1 hybrid seedlings, resulting in negative effects including reduced germination rate, vigor index and emergence speed index. Interestingly Sletr1-2 mutation had almost no effect on the seed quality characteristics of the F1 hybrid lines, suggesting that Sletr1-2 was suitable for producing high quality of hybrid seeds. 展开更多
关键词 ETHYLENE receptor MUTANT hybrid QUALITY SEED TOMATO
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Mutant p53 in cancer therapy-the barrier or the path
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作者 Xiang Zhou Qian Hao Hua Lu 《分子细胞生物学报:英文版》 SCIE CAS CSCD 2019年第4期293-305,共13页
Since wild-type p53 is central for maintaining genomic stability and preventing oncogenesis, its coding gene TP53 is highly mutated in ~50% of human cancers, and its activity is almost abrogated in the rest of cancers... Since wild-type p53 is central for maintaining genomic stability and preventing oncogenesis, its coding gene TP53 is highly mutated in ~50% of human cancers, and its activity is almost abrogated in the rest of cancers. Approximately 80% of p53 mutations are single point mutations with several hotspot mutations. Besides loss of function and dominant-negative effect on the wild-type p53 activity, the hotspot p53 mutants also acquire new oncogenic functions, so-called ‘gain-of-functions’(GOF). Because the GOF of mutant p53 is highly associated with late-stage malignance and drug resistance, these p53 mutants have become hot targets for developing novel cancer therapies. In this essay, we review some recent progresses in better understanding of the role of mutant p53 GOF in chemoresistance and the underlying mechanisms, and discuss the pros and cons of targeting mutant p53 for the development of anti-cancer therapies. 展开更多
关键词 MUTANT p53 GAIN-OF-FUNCTION cancer therapy CHEMORESISTANCE synthetic LETHALITY
单纯疱疹病毒Ⅰ型基因突变株M3株与水痘带状疱疹病毒减毒活疫苗Oka株感染小鼠的生物学特性比较
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作者 柳蕾 徐兴丽 +4 位作者 冯敏 何玉凤 王丽春 张莹 李琦涵 《中国生物制品学杂志》 CAS CSCD 2019年第3期249-254,共6页
目的比较单纯疱疹病毒Ⅰ型(herpes simplex virus typeⅠ,HSV-Ⅰ)基因突变株M3株与水痘带状疱疹病毒(varicella-zoster virus,VZV)减毒活疫苗Oka株感染BALB/c小鼠的生物学特性。方法用不同剂量(103、104、105 PFU/只)的HSV-ⅠM3株及疫... 目的比较单纯疱疹病毒Ⅰ型(herpes simplex virus typeⅠ,HSV-Ⅰ)基因突变株M3株与水痘带状疱疹病毒(varicella-zoster virus,VZV)减毒活疫苗Oka株感染BALB/c小鼠的生物学特性。方法用不同剂量(103、104、105 PFU/只)的HSV-ⅠM3株及疫苗剂量的VZV Oka株以不同方式(腹腔、肌肉、鼻腔、足垫)感染小鼠后,对小鼠的急性临床反应、体重变化、病毒增殖、组织病理损伤及病毒重激活等生物学特性进行比较。结果 HSV-ⅠM3及VZV Oka组小鼠均未见弓背、倒毛、单侧眼失明等急性临床症状,体重均呈逐渐上升趋势;器官组织中病毒拷贝数均处于较低水平,且两组差异无统计学意义(P> 0. 05);小鼠脑组织出现轻微局部炎症细胞浸润、出血、胶质小结或无异常,两组差异较小,脊髓组织均未出现明显异常;神经组织与Vero细胞共培养,均未观察到细胞病变(CPE)。结论 HSV-ⅠM3株与VZV Oka株感染小鼠后的生物学特征无明显差异。 展开更多
关键词 单纯疱疹病毒Ⅰ型 突变株 水痘带状疱疹病毒 减毒活疫苗 小鼠 生物学特性
Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP 预览
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作者 ZHU Xin-xin NI Yong-jing +3 位作者 HE Rui-shi JIANG Yu-mei LI Qiao-yun NIU Ji-shan 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第3期532-538,共7页
We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the mul... We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines(near-isogenic lines;NILs) derived from the original mutant 12 TP, a simple sequence repeat(SSR) marker assay located the 12 TP locus on chromosome arm 2 DL. Four SSR markers were linked to 12 TP and their order was Xcfd233→Xcfd62-12 TP→Xwmc41→Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait ‘three pistils in one floret’ was more than 98% in seven homozygous 12 TP lines;however, the average genetic expressivity in heterozygous F1 plants was about 49%. Thus, the 12 TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12 TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat. 展开更多
关键词 WHEAT multi-pistil MUTANT expressivity MAPPING
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糜子EMS突变体库构建和突变体筛选
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作者 张彬 王喆 +4 位作者 陈利青 禾璐 李红英 乔治军 韩渊怀 《植物遗传资源学报》 CAS CSCD 北大核心 2019年第2期370-376,共7页
糜子(Panicum miliaceum L.)具有抗旱、耐瘠、适应性广、生育期短等特点,是我国北方地区的杂粮作物。糜子高产品种不耐水肥、易倒伏,锄草、间苗费时费工的问题严重影响着农民对糜子生产的积极性和收入。EMS(甲基磺酸乙酯)化学诱变方法... 糜子(Panicum miliaceum L.)具有抗旱、耐瘠、适应性广、生育期短等特点,是我国北方地区的杂粮作物。糜子高产品种不耐水肥、易倒伏,锄草、间苗费时费工的问题严重影响着农民对糜子生产的积极性和收入。EMS(甲基磺酸乙酯)化学诱变方法成本低,操作简单,诱变率高,是作物育种的重要方法。本试验选用糜子品种伊选大红糜进行EMS化学诱变而构建突变体库,对获得的2333个M2材料进行突变频率和全生育期表型多样性等特征的分析。结果显示,所构建的糜子突变体库材料变异类型非常丰富,共发现104个形态性状突变株系,突变频率为4.5%,包括叶色、叶型、株高、生育期、育性、穗型、种皮颜色等性状,该突变体库的构建将为糜子功能基因组学研究和育种提供丰富的突变体资源和新型种质。 展开更多
关键词 糜子 EMS诱变 突变体库
An improved high-oleic line created by chemical mutagen of ‘Huayu 40' 预览
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作者 Xiuzhen Wang Weiguang Ning +8 位作者 Quanxi Sun Yueyi Tang Zhiwei Wang Yongshan Wan Fengzhen Liu Zubo Du Feng Liu Qi Wu Chuantang Wang 《Oil Crop Science-中国油料作物学报英文版》 2019年第1期47-54,共8页
By using chemical mutagenesis,'Huayu 40;a normal-oleic (NO) large-seed high yielding peanut cultivar with wide adaptability was transtormed into mutants with desirable high-oleic (HO) quality traits, comparable pr... By using chemical mutagenesis,'Huayu 40;a normal-oleic (NO) large-seed high yielding peanut cultivar with wide adaptability was transtormed into mutants with desirable high-oleic (HO) quality traits, comparable productivity and adaptability. Of the 3 chemical treatments, viz. 5 mM ethyl methane sulfonate (EMS), 25 mM EMS and 15 mM sodium azide (NaN3), only NaN3 produced HO M2muta nt plants. High oleate phe no type was in itially identified by near in fra red spectroscopy (NIRS) scree ning, and was further confirmed by gas spectrometry (GC) analysis. Sequenee analysis showed that HO plant had a 448G>A mutation in FAD2A and a 441_442insA mutation in FAD2B, respectively. Although the direct use of high yielding and stress resista nt cultivars/li nes to induce quality mutati ons might be a judicious choice to accelerate breeding, our results showed that chemical mutagenesis could quickly create HO pea nut materials and expa nd the genetic bases of HO pea nuts. 展开更多
关键词 high oleic PEANUT CHEMICAL MUTANT FAD2A FAD2B
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Preliminary Study on the Mechanism of Flower Color Variation in White Mutants of Anthurium andeaeanum 预览
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作者 Chengchen SHEN Lan LUO +3 位作者 Xiuxiu LI Yichun HU Xia XIAO Jianzhong TAN 《农业生物技术:英文版》 CAS 2019年第2期1-4,9共5页
[Objectives] This study was conducted to explore the physiological mechanism of flower color variation in the white mutants of Anthurium andeaeanum .[Methods] The seven white mutants of ‘Alabama’ and ‘Turenza’ wer... [Objectives] This study was conducted to explore the physiological mechanism of flower color variation in the white mutants of Anthurium andeaeanum .[Methods] The seven white mutants of ‘Alabama’ and ‘Turenza’ were used as materials to analyze the pigment types, flavonoid types and content and anthocyanin content in the wild type and mutants.[Results] The white spathe mainly contained flavonoids, flavonols, dihydroflavonols and dihydroflavonols;the white mutants of ‘Alabama’ had a higher total flavonoid content than the wild type, while the white mutants of ‘Turenza’ showed an opposite trend;and the spathe of the wild type had the highest anthocyanin content, and the pink part of the two-color mutant or the spathe of the binary color mutant contained trace anthocyanins, while no anthocyanins were detected in the white part of the mutants.[Conclusions] The main cause of the white mutants of A. andeaeanum is related to anthocyanin metabolism. 展开更多
关键词 ANTHURIUM andeaeanum WHITE MUTANT FLAVONOIDS ANTHOCYANIN
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携带小鼠白介素-4截短型突变体基因的5型腺相关病毒载体的构建及外源蛋白的体外表达
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作者 田丽春 朱情情 +2 位作者 刘艾洁 王青青 黄光瑞 《现代生物医学进展》 CAS 2019年第2期211-216,共6页
目的:制备携带碳端结构域缺失的小鼠白介素-4(Interleukin-4,IL-4)基因突变体的5型重组腺相关病毒(recombinant adeno-associated virus, r AAV)并在细胞水平检测其介导的外源蛋白表达情况。方法:通过分子克隆技术构建携带小鼠IL-4碳端2... 目的:制备携带碳端结构域缺失的小鼠白介素-4(Interleukin-4,IL-4)基因突变体的5型重组腺相关病毒(recombinant adeno-associated virus, r AAV)并在细胞水平检测其介导的外源蛋白表达情况。方法:通过分子克隆技术构建携带小鼠IL-4碳端22个氨基酸缺失的突变体的表达质粒pSNAV-mIL-4ΔC22,三质粒共转染法制备重组的5型AAV病毒,体外感染人支气管上皮样细胞系16HBE和BEAS-2B,并通过Western blot和ELISA检测外源蛋白表达。结果:DNA测序表明构建的小鼠IL-4碳端第118位氨基酸位点处截短的突变体基因表达序列正确无误,制备的重组病毒载体r AAV5-mIL-4ΔC22滴度约为3×1011vg/m L。r AAV5-GFP感染16HBE和BEAS-2B细胞后36小时开始可见持续稳定的荧光蛋白表达,重组病毒r AAV5-mIL-4ΔC22感染16HBE和BEAS-2B细胞后外源蛋白在培养上清中呈分泌型表达。结论:本研究成功构建了携带小鼠IL-4碳端结构域缺失型突变体的AAV表达质粒并制备了重组病毒r AAV5-mIL-4ΔC22,该病毒可有效转染16HBE和BEAS-2B细胞并介导外源基因分泌表达截短型小鼠IL-4突变体蛋白。 展开更多
关键词 白介素-4 突变体 腺相关病毒 基因治疗
1-DE/MS Analysis of the Proteins Related to Spathe Color Variation in Anthurium andraeanum ‘Madural’ 预览
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作者 Le GAO Lan LUO +3 位作者 Chengchen SHEN Yichun HU Xia XIAO Jianzhong TAN 《农业生物技术:英文版》 CAS 2019年第2期18-20,24共4页
In order to understand the mechanism of spathe color variation in Anthurium andraeanum at the protein level, the leaves, inflorescences and spathes of the wild type and two mutants of A. andraeanum ‘Madural’ were us... In order to understand the mechanism of spathe color variation in Anthurium andraeanum at the protein level, the leaves, inflorescences and spathes of the wild type and two mutants of A. andraeanum ‘Madural’ were used as research objects in which the differential expression of proteins related to flower color mutants was analyzed by one-dimensional electrophoresis and mass spectrometry (1-DE/MS). The 1-DE patterns showed that the protein components expressed highly in spathes were mainly concentrated in the molecular weight range of 20-42 kD, and differential bands were detected between the wild type and the mutant, while no significant differences were detected in the leaf and inflorescence proteins. According to the results of mass spectrometry analysis of the differential bands, 21 known functional proteins involved in life processes such as glucose metabolism, resistance, cytoskeleton, gene regulation and signal transduction were identified. It showed that in addition to the influences from anthocyanidins, the spathe color variation of A. andraeanum ‘Madural’ is also regulated by a variety of metabolic pathway-related proteins. 展开更多
关键词 ANTHURIUM andraeanum FLOWER color MUTANT Differential protein Gel ELECTROPHORESIS Mass SPECTROMETRY
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大豆7S球蛋白α'亚基缺失新种质中黄608的分子鉴定 预览
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作者 李俊英 孙如建 +4 位作者 李忠峰 魏中艳 任玉龙 王俊 邱丽娟 《作物学报》 CAS CSCD 北大核心 2019年第1期18-25,共8页
大豆7S球蛋白α'亚基含量与大豆的营养品质和加工特性关系密切。本研究利用聚丙烯酰胺凝胶电泳(SDS-PAGE)和免疫印迹法(Western blot)从中品661的EMS突变库中筛选出α'亚基缺失突变体中黄608。利用中黄608与登科1号创建了一个由... 大豆7S球蛋白α'亚基含量与大豆的营养品质和加工特性关系密切。本研究利用聚丙烯酰胺凝胶电泳(SDS-PAGE)和免疫印迹法(Western blot)从中品661的EMS突变库中筛选出α'亚基缺失突变体中黄608。利用中黄608与登科1号创建了一个由210个个体组成的F2分离群体。遗传分析表明, α'亚基缺失由1对隐性单基因控制。利用连锁分析方法将该基因定位于第10染色体标记SSR10-1489 与SSR10-1612之间, 其中, 包括控制α'亚基合成基因Cgy-1(Glyma.10G246300), 序列分析发现, 中黄608在Cgy-1第1外显子第84个碱基发生单碱基突变(G^84→A^84), 导致氨基酸翻译提前终止。根据新发现的变异位点开发了共显性分子标记, 并检测F2个体基因型, 结果表明, Cgy-1基因型与α'亚基表型共分离。本研究不仅为大豆优质育种提供了新材料, 同时也为分子育种提供了技术支持。 展开更多
关键词 大豆 α'亚基 突变体 7S球蛋白 dCAPs标记
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水稻“9311”突变体库的氯酸盐毒性筛选与分析
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作者 刘石锋 陈倩 +2 位作者 洪广成 张汉马 秦小健 《重庆师范大学学报:自然科学版》 CAS 北大核心 2019年第2期107-111,F0003共6页
【目的】用氯酸盐筛选水稻(Oryza sativa)突变体库,分析氯酸盐对水稻的毒性。【方法】使用化学诱变剂甲基磺酸乙酯(Ethyl methanesulphonate,EMS)处理水稻材料“9311”种子,构建完成了水稻“9311”突变体库。【结果】通过水培法并利用... 【目的】用氯酸盐筛选水稻(Oryza sativa)突变体库,分析氯酸盐对水稻的毒性。【方法】使用化学诱变剂甲基磺酸乙酯(Ethyl methanesulphonate,EMS)处理水稻材料“9311”种子,构建完成了水稻“9311”突变体库。【结果】通过水培法并利用氯酸盐的特性对诱变材料进行筛选,共筛选了2 361份诱变M2代材料,且与野生型相比获得了40份对氯酸盐表现差异敏感的材料。【结论】研究结果为进一步的材料创建与相关基因克隆提供了基础,也为水稻硝酸盐吸收与转化相关分子机理的研究奠定了基础。 展开更多
关键词 水稻 突变体 氯酸盐 差异筛选
玉米穗发芽突变体vp-like8的遗传分析及突变基因鉴定 预览
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作者 王瑞 陈阳松 +3 位作者 孙明昊 张秀艳 杜依聪 郑军 《作物学报》 CAS CSCD 北大核心 2019年第5期656-661,共6页
玉米突变体vp-like8具有明显的穗发芽性状且能稳定遗传,遗传分析表明该突变性状受隐性单基因控制。用vp-like8与自交系郑58杂交构建F2遗传定位群体,利用BSR-Seq方法,将基因初定在玉米第3染色体160.4 Mb~165.6Mb区间内。参考玉米基因组信... 玉米突变体vp-like8具有明显的穗发芽性状且能稳定遗传,遗传分析表明该突变性状受隐性单基因控制。用vp-like8与自交系郑58杂交构建F2遗传定位群体,利用BSR-Seq方法,将基因初定在玉米第3染色体160.4 Mb~165.6Mb区间内。参考玉米基因组信息,发现已报道的穗发芽基因Vp1位于此定位区间内。分别利用vp1、vp-like8的杂合突变体进行等位测验,发现杂交后代中正常与穗发芽籽粒符合3∶1遗传分离比。经序列分析,发现vp-like8突变体中Vp1基因在第2内含子有343 bp碱基的缺失,且第3内含子有222 bp重复序列的插入,而已报道的vp1突变体只在第2个内含子有343 bp碱基的缺失。通过实时定量PCR检测发现,与正常籽粒相比,vp-like8与Vp1突变籽粒中Vp1基因的转录水平均明显降低。以上证据表明,vp-like8是一个新的Vp1基因等位突变体。 展开更多
关键词 玉米 穗发芽 突变体 VP1 基因定位
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糖橙新品种‘橘湘元’的选育 预览
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作者 龙桂友 黄洪 +3 位作者 张凤 Alessandra Gentile 邓子牛 李娜 《果树学报》 CAS CSCD 北大核心 2019年第3期388-391,共4页
‘橘湘元’是从埃及糖橙芽变中选育的无核糖橙新品种。果实圆球形,果形指数为0.95。平均单果质量173 g,大小较均匀,果面较光滑,有光泽,果肉橙黄色,平均每果实含0.4粒种子。可食率达77.09%,果实出汁率54.6%,可溶性固形物13.63%,可滴定酸0... ‘橘湘元’是从埃及糖橙芽变中选育的无核糖橙新品种。果实圆球形,果形指数为0.95。平均单果质量173 g,大小较均匀,果面较光滑,有光泽,果肉橙黄色,平均每果实含0.4粒种子。可食率达77.09%,果实出汁率54.6%,可溶性固形物13.63%,可滴定酸0.14%,维生素C含量60.57 mg·100 mL^-1。‘橘湘元’在树形树势、叶片大小、花瓣宽度等方面与埃及糖橙均存在明显差异。果实生育期260 d,在湖南省永兴县11月中下旬成熟。SSR结果分析表明‘橘湘元’在300 bp处较‘埃及糖橙’多1条带,具有遗传特异性。‘橘湘元’可在山地和旱地种植,表现生长快,抗性较强。 展开更多
关键词 柑橘 新品种 '橘湘元’ 芽变 无核 低酸
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广藿香青枯病菌Tn5转座子插入突变体的构建
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作者 王亚琴 张宇瑶 +4 位作者 贺红 李颛 邓志成 金华 黎广卫 《中国中药杂志》 CAS CSCD 北大核心 2019年第1期77-81,共5页
该研究以从感染了青枯病的广藿香植株中分离的青枯菌菌株PRS-84为供试菌株,利用电击转化法,对青枯菌进行Tn5转座子插入突变,在含卡那霉素的培养基上筛选抗性克隆。对抗性克隆进行卡那霉素抗性基因的PCR鉴定,并利用反向PCR技术获得转化子... 该研究以从感染了青枯病的广藿香植株中分离的青枯菌菌株PRS-84为供试菌株,利用电击转化法,对青枯菌进行Tn5转座子插入突变,在含卡那霉素的培养基上筛选抗性克隆。对抗性克隆进行卡那霉素抗性基因的PCR鉴定,并利用反向PCR技术获得转化子Tn5转座子插入位点的侧翼序列并进行序列测定与分析。结果表明,青枯菌感受态细胞经电击转化后,获得了抗性克隆。经PCR扩增,抗性克隆在预计的约700 bp处出现特异性条带。反向PCR扩增获得了转化子Tn5转座子插入位点的侧翼序列,经序列测定及同源性分析,初步推测3个突变体的Tn5转座子插入位点基因分别为typ A基因、rec O基因和gid A基因。该研究建立了广藿香青枯病菌Tn5转座子插入突变体系,获得了青枯菌Tn5转座子插入突变体,为构建广藿香青枯菌突变体库及发现青枯菌致病基因奠定了基础。 展开更多
关键词 广藿香 青枯菌 Tn5转座子 电击转化 突变体
磷脂酶参与二萜类化合物冬凌草甲素(oridonin)对拟南芥的化感作用
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作者 安炎黄 赵启安 +3 位作者 陈璐 赵敏 张莉环 杨宁 《生态学杂志》 CAS CSCD 北大核心 2019年第4期995-1003,共9页
为探究二萜类化合物冬凌草甲素(oridonin)对拟南芥的化感作用机制,本研究以冬凌草甲素为供体,以哥伦比亚野生型拟南芥WT和3个拟南芥磷脂酶A1(PLA1)、磷脂酶A2(PLA2)、磷脂酶C(PLC)的突变体株系pla1、pla2和plc1为受体植物,通过施加不同... 为探究二萜类化合物冬凌草甲素(oridonin)对拟南芥的化感作用机制,本研究以冬凌草甲素为供体,以哥伦比亚野生型拟南芥WT和3个拟南芥磷脂酶A1(PLA1)、磷脂酶A2(PLA2)、磷脂酶C(PLC)的突变体株系pla1、pla2和plc1为受体植物,通过施加不同浓度的冬凌草甲素(10、60μmol·L-1),对4种拟南芥株系的种子萌发率、幼苗生长状况、相对电导率(EL)、丙二醛(MDA)含量以及3种磷脂酶活性进行了检测。结果表明:外施不同浓度的冬凌草甲素后,拟南芥种子萌发受到抑制,PLA1和PLA2参与此抑制过程;根长和下胚轴生长呈现典型的'低促高抑'趋势,PLA1参与其对根的调控,PLC参与其对下胚轴的调控;3种磷脂酶PLA1、PLA2和PLC酶活性均有不同程度的变化;EL和MDA增大且与浓度和时间呈正相关,PLA2正调控10μmol·L-1冬凌草甲素增大EL的过程,PLA1和PLA2共同响应60μmol·L-1冬凌草甲素增大EL的过程;PLA1和PLA2共同作用正调控MDA积累过程;冬凌草甲素对植物的化感作用与磷脂酶有关,植物通过调控磷脂酶响应了冬凌草甲素的部分化感作用,且不同的磷脂酶在调控植物响应冬凌草甲素的生长过程中具有不同的作用。 展开更多
关键词 冬凌草甲素 磷脂酶 化感作用 突变体
华癸根瘤菌urtB基因突变株的构建与共生固氮表型分析
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作者 周雪娟 马春草 +2 位作者 李亚楠 胡倡 李友国 《微生物学报》 CAS CSCD 北大核心 2019年第2期374-385,共12页
【目的】尿素ABC转运体透性酶亚基编码基因urtB可能参与尿素代谢及支链氨基酸转运;本文旨在获得实验证据阐明urtB基因对华癸根瘤菌结瘤和固氮的影响,为深入研究其功能机制提供一定的科学依据。【方法】利用生物信息学分析urtB基因的结... 【目的】尿素ABC转运体透性酶亚基编码基因urtB可能参与尿素代谢及支链氨基酸转运;本文旨在获得实验证据阐明urtB基因对华癸根瘤菌结瘤和固氮的影响,为深入研究其功能机制提供一定的科学依据。【方法】利用生物信息学分析urtB基因的结构特征及生物学功能,通过荧光定量检测urtB基因在自生和共生条件下的时空表达特征和启动子原位表达技术检测urtB基因组织表达特征,采用插入突变构建urtB突变株,通过植物盆栽并结合添加氮素处理,检测与分析突变体的共生固氮表型变化。【结果】分析表明urtB基因对于氮素转运非常重要,在共生条件下的表达水平比自生培养条件下显著上调表达;在成熟根瘤的固氮区中大量表达;正确构建和筛选获得了根瘤菌urtB突变株;接种urtB突变株与野生型菌株7653R相比较,突变体根瘤发育异常;植株地上部分生物量和根瘤固氮酶活性显著降低;添加氮素可恢复其共生缺陷表型。【结论】华癸中慢生根瘤菌urtB基因可能通过影响根瘤中氮转运或同化,进而在根瘤发育与共生固氮中发挥重要作用。 展开更多
关键词 华癸中慢生根瘤菌 urtB 突变体 共生固氮
KRAS基因突变与分化型甲状腺癌131I放疗耐受性及预后的关系
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作者 冯志平 陈富坤 +6 位作者 杨传周 陈婷 朱家伦 刘超 吕娟 陆建梅 邓智勇 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2019年第2期213-219,共7页
目的:探究KRAS基因突变与分化型甲状腺癌(differentiated thyroid carcinoma,DTC)131I放疗疗效和预后的相关性,并阐明其可能的机制。方法:收集经131I放射治疗DTC临床组织样本,聚合酶链反应-单链构象分析法(single strand conformationpo... 目的:探究KRAS基因突变与分化型甲状腺癌(differentiated thyroid carcinoma,DTC)131I放疗疗效和预后的相关性,并阐明其可能的机制。方法:收集经131I放射治疗DTC临床组织样本,聚合酶链反应-单链构象分析法(single strand conformationpolymorphism analysis of polymerase chain reaction products,PCR-SSCP)检测KRAS的遗传突变;采用qPCR和Wb检测p21蛋白的表达水平;亚致死剂量的131I放射治疗DTC细胞系,采用CCK-8、流式细胞术(FCM)、Transwell实验检测细胞活力的变化,并通过动物模型验证。结果:131I放射治疗耐受DTC患者的KRAS基因突变增加(P<0.01),KRAS基因突变导致p21蛋白表达下调(P<0.05),且与DTC临床分期及预后较差相关(P<0.05,P<0.01)。体内外实验证明,亚致死剂量的131I放射治疗导致DTC细胞KRAS基因的突变率增加、p21蛋白的表达水平降低,导致DTC细胞产生131I放射耐受,而超表达KRAS基因显著提高p21的表达,抑制肿瘤增长及转移。结论:KRAS基因突变与DTC临床分期及131I放射耐受相关,亚致死剂量131I放射治疗DTC促进KRAS基因突变产生放射耐受,而超表达KRAS基因能够提高DTC对131I放射治疗的敏感性。 展开更多
关键词 分化型甲状腺癌 131I放射治疗 KRAS基因 突变 放疗耐受性
Mutant p53 in colon cancer
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作者 Mizuho Nakayama Masanobu Oshima 《分子细胞生物学报:英文版》 SCIE CAS CSCD 2019年第4期267-276,共10页
The accumulation of genetic alterations in driver genes is responsible for the development and malignant progression of colorectal cancer. Comprehensive genome analyses have revealed the driver genes, including APC, K... The accumulation of genetic alterations in driver genes is responsible for the development and malignant progression of colorectal cancer. Comprehensive genome analyses have revealed the driver genes, including APC, KRAS, TGFBR2, and TP53, whose mutations are frequently found in human colorectal cancers. Among them, the p53 mutation is found in ~60% of colorectal cancers, and a majority of mutations are missense-type at ‘hot spots’, suggesting an oncogenic role of mutant p53 by ‘gain-of-function’ mechanisms. Mouse model studies have shown that one of these missense-type mutations, p53 R270H (corresponding to human R273H), causes submucosal invasion of intestinal tumors, while the loss of wild-type p53 has a limited effect on the invasion process. Furthermore, the same mutant p53 promotes metastasis when combined with Kras activation and TGF-β suppression. Importantly, either missense-type p53 mutation or loss of wild-type p53 induces NF-κB activation by a variety of mechanisms, such as increasing promoter accessibility by chromatin remodeling, which may contribute to progression to epithelial–mesenchymal transition. These results indicate that missense-type p53 mutations together with loss of wild-type p53 accelerate the late stage of colorectal cancer progression through the activation of both oncogenic and inflammatory pathways. Accordingly, the suppression of the mutant p53 function via the inhibition of nuclear accumulation is expected to be an effective strategy against malignant progression of colorectal cancer. 展开更多
关键词 colon cancer missense-type mutant p53 GAIN-OF-FUNCTION mouse model NF-κB MULTISTEP TUMORIGENESIS ORGANOIDS
Tumor suppressor p53 and metabolism
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作者 Juan Liu Cen Zhang +1 位作者 Wenwei Hu Zhaohui Feng 《分子细胞生物学报:英文版》 SCIE CAS CSCD 2019年第4期284-292,共9页
p53 plays a key role in tumor suppression. The tumor suppressive function of p53 has long been attributed to its ability to induce apoptosis, cell cycle arrest, and senescence in cells. However, recent studies suggest... p53 plays a key role in tumor suppression. The tumor suppressive function of p53 has long been attributed to its ability to induce apoptosis, cell cycle arrest, and senescence in cells. However, recent studies suggest that other functions of p53 also contribute to its role as a tumor suppressor, such as its function in metabolic regulation. p53 regulates various metabolic pathways to maintain the metabolic homeostasis of cells and adapt cells to stress. In addition, recent studies have also shown that gain-offunction (GOF) mutant p53 proteins drive metabolic reprogramming in cancer cells, contributing to cancer progression. Further understanding of p53 and its GOF mutants in metabolism will provide new opportunities for cancer therapy. 展开更多
关键词 P53 tumor SUPPRESSOR MUTANT P53 GAIN-OF-FUNCTION METABOLISM
角蛋白降解能力差异菌株筛选体系的建立 预览
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作者 郝亚楠 宋枭枭 +1 位作者 周骏驰 曹张军 《工业微生物》 CAS 2019年第2期7-11,共5页
以微生物降解为主的羽毛生物炼制是资源化利用其的重要手段之一,但机理不明是限制其进一步应用的瓶颈。突变体库筛选性状变异菌株的正向遗传学是研究分子机理的常用方案,该方案要求高效可靠的筛选方法。然而,目前依靠测酶活方法来决定... 以微生物降解为主的羽毛生物炼制是资源化利用其的重要手段之一,但机理不明是限制其进一步应用的瓶颈。突变体库筛选性状变异菌株的正向遗传学是研究分子机理的常用方案,该方案要求高效可靠的筛选方法。然而,目前依靠测酶活方法来决定降解能力,费时费力,难以用于大规模筛选。本文通过优化羽毛培养基成分及接种菌浓度,建立了一种用于筛选不同角蛋白降解能力微生物的筛选体系。利用该体系分析由mini Tn10构建的S.maltophinia DHHJ突变体库,得到了6株羽毛角蛋白降解能力减弱的突变体,筛选结果与酶活测试100%一致。该研究结果为寻找与角蛋白降解相关基因奠定了基础。 展开更多
关键词 角蛋白降解菌 突变体 筛选体系
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