期刊文献+
共找到190篇文章
< 1 2 10 >
每页显示 20 50 100
文章速递Short-Term Visual Experience Leads to Potentiation of Spontaneous Activity in Mouse Superior Colliculus 认领
1
作者 Qingpeng Yu Hang Fu +2 位作者 Gang Wang Jiayi Zhang Biao Yan 《神经科学通报:英文版》 SCIE CAS CSCD 2021年第3期353-368,共16页
Spontaneous activity in the brain maintains an internal structured pattern that reflects the external environment,which is essential for processing information and developing perception and cognition.An essential prer... Spontaneous activity in the brain maintains an internal structured pattern that reflects the external environment,which is essential for processing information and developing perception and cognition.An essential prerequisite of spontaneous activity for perception is the ability to reverberate external information,such as by potentiation.Yet its role in the processing of potentiation in mouse superior colliculus(SC)neurons is less studied.Here,we used electrophysiological recording,optogenetics,and drug infusion methods to investigate the mechanism of potentiation in SC neurons.We found that visual experience potentiated SC neurons several minutes later in different developmental stages,and the similarity between spontaneous and visually-evoked activity increased with age.Before eye-opening,activation of retinal ganglion cells that expressed ChR2 also induced the potentiation of spontaneous activity in the mouse SC.Potentiation was dependenton stimulus number and showed feature selectivity for direction and orientation.Optogenetic activation of parvalbumin neurons in the SC attenuated the potentiation induced by visual experience.Furthermore,potentiation in SC neurons was blocked by inhibiting the glutamate transporter GLT1.These results indicated that the potentiation induced by a visual stimulus might play a key role in shaping the internal representation of the environment,and serves as a carrier for short-term memory consolidation. 展开更多
关键词 Spontaneous activity Visual experience Potentiation Superior colliculus Feature selectivity Glutamate homeostasis
龙蒲定神丸对小鼠行为学的影响及其急性毒理学研究 认领
2
作者 袁岳鹏 宏亚丽 +1 位作者 万美霞 吴国泰 《陇东学院学报》 2021年第2期62-64,共3页
目的:观察龙蒲定神丸(DSW)对小鼠行为学的影响并考察其急性毒性。方法:将60只小鼠随机分为5组,每组12只,阴性对照组(生理盐水)、阳性对照组(养血安神片2.36g·kg-1)、DSW高剂量组(5.71g·kg-1)、中剂量组(2.86g·kg-1)、低... 目的:观察龙蒲定神丸(DSW)对小鼠行为学的影响并考察其急性毒性。方法:将60只小鼠随机分为5组,每组12只,阴性对照组(生理盐水)、阳性对照组(养血安神片2.36g·kg-1)、DSW高剂量组(5.71g·kg-1)、中剂量组(2.86g·kg-1)、低剂量组(1.43g·kg-1),灌胃给药。通过小鼠的自主活动实验、避暗实验以及戊巴比妥钠催眠实验,观察小鼠运动行为的变化;按改良寇氏法测定其LD 50,评价DSW的安全性。结果:DSW高剂量组小鼠自主活动量和站立次数明显降低,DSW低剂量组小鼠站立次数明显减少;DSW高、中剂量组小鼠犯错误次数明显减少,DSW中、低剂量组小鼠逃避潜伏期明显缩短;DSW高、中剂量组小鼠入睡率明显增加,DSW各剂量组小鼠入睡潜伏期明显缩短。DSW给药剂量2.86g·kg-1、11.44g·kg-1、34.32g·kg-1,均未见动物死亡。结论:DSW能改善小鼠的自主活动、增强学习记忆,并具有镇静催眠作用;DSW的毒性甚小,在规定剂量范围内应用是安全可靠的。 展开更多
关键词 DSW 自主活动 学习记忆 镇静催眠 急性毒性
在线阅读 下载PDF
低温环境下禁食对小鼠自发运动及腓肠肌中PGC-lα、ERRα表达的影响 认领
3
作者 刘洋 姚睿智 +5 位作者 刘鹏 史宏昭 胡亚婕 徐彬 吕红明 李士泽 《中国兽医学报》 CAS 北大核心 2021年第2期295-301,326,共8页
应用旷场试验测定不同时长4℃冷暴露下采食和禁食C57BL/6小鼠的自发运动,并结合骨骼肌中PGC-lα与ERRα表达水平,探讨低温环境下禁食对C57BL/6小鼠自发运动的影响。结果显示,低温环境下禁食对C57BL/6小鼠的自发运动并无显著性影响,但焦... 应用旷场试验测定不同时长4℃冷暴露下采食和禁食C57BL/6小鼠的自发运动,并结合骨骼肌中PGC-lα与ERRα表达水平,探讨低温环境下禁食对C57BL/6小鼠自发运动的影响。结果显示,低温环境下禁食对C57BL/6小鼠的自发运动并无显著性影响,但焦虑情绪12 h差异显著(P<0.05),探索行为8 h和12 h差异极显著(P<0.01);腓肠肌中PGC-1α表达在4,8 h时差异极显著(P<0.01),12 h差异显著(P<0.05);ERRα表达在4,6 h时差异极显著(P<0.01)。结果表明,低温环境下禁食对C57BL/6小鼠自发运动未出现明显影响,而对小鼠焦虑情绪、探索行为和腓肠肌中PGC-lα、ERRα表达存在不同程度的影响,相比于禁食而言,冷刺激对小鼠机体的影响更大。 展开更多
关键词 冷暴露 旷场试验 自发运动 探索行为 PGC-1Α ERRα
Heterogeneity of the Neural Composition of Cortical Regions as a Condition for a Wide Range Regulating Spontaneous Activity 认领
4
作者 Yulia S. Mednikova Anna V. Rogal 《行为与脑科学期刊(英文)》 2020年第5期220-236,共17页
On the slices of sensorimotor and frontal cortex in layer V of guinea pigs the diversity of neuronal spontaneous activity, the mechanism of its origin and functional specificity were studied. In both regions, neurons ... On the slices of sensorimotor and frontal cortex in layer V of guinea pigs the diversity of neuronal spontaneous activity, the mechanism of its origin and functional specificity were studied. In both regions, neurons that did not have spontaneous activity predominated (39% in the sensorimotor cortex and 32% in the frontal cortex) over neurons with any other firing levels. The iontophoretic application of the excitatory transmitter, glutamate, caused activation spike reactions in all registered neurons;moreover, short-term activation reactions to glutamate had a significantly longer after-discharge in neurons of the frontal cortex (up to 2500 ms and more) compared to reactions of neurons of the sensorimotor region. This means that postexcitatory hyperpolarization in nerve cells of the frontal cortex is less expressed and, therefore, they have a lower density of K+ channels on their membranes. With an increase in the level of spontaneous activity, K+ membrane permeability decreases, which is confirmed by the appearance of a long activation reaction to acetylcholine (which blocks K+ channels), exactly when spontaneous firing appears in “silent” neurons. Despite the fact that spontaneous activity is formed by glutamatergic excitation, its considerable diversity is associated with the structural and membrane characteristics of neurons, which determine the different degrees of EPSPs attenuation on the way of moving along dendrites. Acetylcholine regulates this process in different ways, in accordance with different states of K+ membrane permeability. Therefore, the lower content of K+ channels on the membranes of neurons of the frontal cortex does not allow regulating spontaneous activity in the same range as occurs in neurons of the sensorimotor region. The presence of a high proportion of spontaneously inactive neurons in the cortex (higher in the sensorimotor cortex) suggests that cortical neurons are generally characterized by a high density of K+ channels and a significant increase in firing response to acetyl 展开更多
关键词 SENSORIMOTOR CORTEX FRONTAL CORTEX Spontaneous Activity Glutamate Post-Activation AFTEREFFECT ACETYLCHOLINE
在线阅读 免费下载
下丘脑LHA-VMH Ghrelin信号通路对肥胖大鼠摄食选择、胃肠道运动及自发活动的影响及机制研究 认领
5
作者 邢金磊 冷慧 +4 位作者 王茜 高胜利 孙向荣 郭菲菲 徐珞 《现代生物医学进展》 CAS 2020年第3期439-444,433共7页
目的:探究下丘脑外侧区(LHA)-腹内侧核(VMH)ghrelin信号通路对肥胖大鼠的摄食选择、胃肠道运动及自发活动的影响。方法:采用免疫组织化学方法检测大鼠LHA中ghrelin受体的表达;观察LHA注射ghrelin对大鼠摄食选择胃肠道运动及自发活动的影... 目的:探究下丘脑外侧区(LHA)-腹内侧核(VMH)ghrelin信号通路对肥胖大鼠的摄食选择、胃肠道运动及自发活动的影响。方法:采用免疫组织化学方法检测大鼠LHA中ghrelin受体的表达;观察LHA注射ghrelin对大鼠摄食选择胃肠道运动及自发活动的影响;电损毁VMH,观察LHA注射ghrelin对大鼠摄食的影响。结果:免疫组化结果显示,大鼠下丘脑LHA中存在ghrelin受体,且LHA-VMH之间存在纤维投射;大鼠LHA微量注射ghrelin后,肥胖(DIO)大鼠及肥胖抵抗(DR)大鼠的正常饮食、高脂饮食及高糖饮食均高于正常大鼠,但预注射ghrelin受体拮抗剂[D-Lys3]-GHRP-6 (DLS)能够阻断这种作用;而电损毁大鼠VMH,显著减弱了ghrelin对正常大鼠、DIO大鼠及DR大鼠的促摄食作用。大鼠LHA微量注射ghrelin后,正常大鼠、DIO大鼠及DR大鼠的自发活动中,X轴、Y轴方向上的活动增加,且总活动增加,但Z轴方向上活动无明显改变;此外,LHA注射ghrelin,DIO大鼠及DR大鼠的胃肠道转运速率明显加快,且DR大鼠胃肠道转运速率增加更为明显,而预注射ghrelin受体拮抗剂[D-Lys3]-GHRP-6(DLS)显著阻断ghrelin的促胃肠道转运作用。结论:下丘脑LHA-VMH ghrelin信号通路参与调节正常大鼠、DIO及DR大鼠的摄食选择、胃肠道运动及自发活动。 展开更多
关键词 GHRELIN 下丘脑外侧区 腹内侧核 摄食选择 胃运动 自发活动
Altered spontaneous brain activity pattern in patients with ophthalmectomy: an resting-state fMRI study 认领
6
作者 Bing Zhang Biao Li +7 位作者 Rong-Qiang Liu Yong-Qiang Shu You-Lan Min Qing Yuan Pei-Wen Zhu Qi Lin Lei Ye Yi Shao 《国际眼科杂志:英文版》 SCIE CAS 2020年第2期263-270,共8页
●AIM:To use the voxel-wise degree centrality(DC)method to explore the underlying functional network brain-activity in patients with ophthalmectomy.●METHODS:A total of 32 ophthalmic surgery patients(10 women and 22 m... ●AIM:To use the voxel-wise degree centrality(DC)method to explore the underlying functional network brain-activity in patients with ophthalmectomy.●METHODS:A total of 32 ophthalmic surgery patients(10 women and 22 men),and 32 healthy subjects(10 women and 22 men)highly matched in gender,age,and the same operation method.Everyone experienced a restingstate functional magnetic resonance imaging scan.The spontaneous brain activity could be assessed by DC.Correlation analysis was used to explore the relationships between the average DC signal values and behavior performance in different regions.Receiver operating characteristic(ROC)cur ve analysis was utilized to differentiate between ophthalmectomy patients and healthy controls(HCs).●RESULTS:Compared with HCs,ophthalmectomy patients had greatly reduced DC values in left lingual gyrus,bilateral lingual lobe,left cingulate gyrus,and increased DC values of left cerebellum posterior lobe,left middle frontal gyrus1,right supramarginal gyrus,left middle frontal gyrus2,right middle frontal gyrus.However,we did not find that there was a correlation between the average DC values from various brain regions and clinical manifestations.●CONCLUSION:Dysfunction may be caused by ophthalmectomy in lots of cerebral areas,which may show the potential pathological mechanism of ophthalmectomy and it is beneficial to clinical diagnosis. 展开更多
关键词 DEGREE CENTRALITY ophthalmectomy RESTING state SPONTANEOUS BRAIN activity
一种非苯二氮[艹卓]点的新型催眠物质活性研究 认领
7
作者 张毅 石童 +7 位作者 陈学军 张瑞华 邓诗坤 崔雅岚 时梦 徐建富 王陈 李丽琴 《中南药学》 CAS 2020年第12期1945-1949,共5页
目的采用膜电位高通量筛选技术进行化合物库筛选及活性研究,以期发现不同于苯二氮[艹卓]类结构的新型催眠物质。方法采用高通量实时荧光膜电位检测方法对化合物库进行筛选,获得候选化合物;通过膜片钳电生理和放射配基受体结合检测技术... 目的采用膜电位高通量筛选技术进行化合物库筛选及活性研究,以期发现不同于苯二氮[艹卓]类结构的新型催眠物质。方法采用高通量实时荧光膜电位检测方法对化合物库进行筛选,获得候选化合物;通过膜片钳电生理和放射配基受体结合检测技术进行体外活性评价;采用戊巴比妥钠阈下睡眠检测和小鼠自发活动进行体内活性评价。结果通过膜电位检测方法从2288个化合物中筛选得到EC50值低于阳性对照地西泮[EC50=(4.43±0.41)μmol·L^-1]的化合物A11[EC50=(1.98±0.47)μmol·L^-1];通过膜片钳检测其体外活性EC50=(20.47±0.45)μmol·L^-1;[3H]-氟硝西泮受体结合检测技术表明该化合物为非苯二氮[艹卓]类化合物;戊巴比妥钠阈下睡眠时间检测实验表明化合物A11可明显缩短睡眠潜伏期(P<0.05),延长睡眠持续时间;A11在0.5、1、2 mg·kg^-1剂量下对90 min后小鼠自发活动有明显抑制作用(P<0.05,P<0.01)。结论本研究基于GABAAR高通量筛选获得的化合物A11具有较强的睡眠活性,可能是一种不同于苯二氮[艹卓]类药物作用位点的新型催眠物质。 展开更多
关键词 GABAAR 膜电位检测 电生理 [3H]-氟硝西泮 自发活动
在线阅读 免费下载
Dynamic Alterations in Spontaneous Brain Activity in Mothers:A Resting-State Functional Magnetic Resonance Imaging Study 认领
8
作者 Kaihua Zhang Mengxing Wang +4 位作者 Jilei Zhang Junyao Xie Xueyun Su Xiaoxia Du Zhong Chen 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第4期766-770,共5页
Becoming a mother is one of the most monumental experiences in a woman's lifetime. Women typically bear the primary caregiving responsibility for their infants, and they undergo numerous changes both mentally and ... Becoming a mother is one of the most monumental experiences in a woman's lifetime. Women typically bear the primary caregiving responsibility for their infants, and they undergo numerous changes both mentally and physically, including behavioral, emotional, and hormonal changes, during the postpartum period. Studies have indicated that hormonal, experiential, and temporal factors significantly regulate emotional and cognitive brain functions during the postpartum period [1]. The determination of the neural basis of a maternal brain is critically important for understanding mother-infant attachments and thus perpetuating the human species. Until recently, limited investigations of the neuroanatomical and functional status of mothers have contributed to understanding the processing of maternal behaviors. 展开更多
关键词 DYNAMIC Alterations SPONTANEOUS BRAIN ACTIVITY IMAGING STUDY
Abnormal spontaneous brain activity in patients with non-arteritic anterior ischemic optic neuropathy detected using functional magnetic resonance imaging 认领
9
作者 Peng-De Guo Peng-Bo Zhao +9 位作者 Han Lv Feng-Yuan Man Yan Su Jing Zhao Ming Liu Yun-Xiang Chen Yan Wang Hai-Qin Hua Ling-Ling Cai Jian Zhou 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第6期741-743,共3页
To the Editor: Non-arteritic anterior ischemic optic neuropathy (NAION), characterized by a sudden onset of unilateral painless visual loss and a swollen optic disc, is the most common form of acute optic neuropathy i... To the Editor: Non-arteritic anterior ischemic optic neuropathy (NAION), characterized by a sudden onset of unilateral painless visual loss and a swollen optic disc, is the most common form of acute optic neuropathy in patients over 50 years of age.[1] The pathogenesis of NAION remains unclear, but most histopathological studies support the concept of vasculopathic occlusion in the region of the short posterior ciliary arteries.[2] Functional magnetic resonance imaging (fMRI) is one of the most widely used non-invasive techniques for measuring brain function over time in vivo. To the best of our knowledge, only one study has investigated the effects of stimulating the affected eye in the extra-visual areas in NAION in humans.[3]However, this previous study only examined task-state fMRI, and lacked a measure of resting-state fMRI (rs-fMRI). Thus, it remains unclear whether regional spontaneous brain changes are limited to the visual system. 展开更多
关键词 ABNORMAL SPONTANEOUS brain activity magnetic resonance imaging NAION REMAINS unclear
ASAF: altered spontaneous activity fingerprinting in Alzheimer's disease based on multisite fMRI 认领 被引量:1
10
作者 Jiachen Li Dan Jin +20 位作者 Ang Li Bing Liu Chengyuan Song Pan Wang Dawei Wang Kaibin Xu Hongwei Yang Hongxiang Yao Bo Zhou Alexandre Bejanin Gael Chetelat Tong Han Jie Lu Qing Wang Chunshui Yu Xinqing Zhang Yuying Zhou Xi Zhang Tianzi Jiang Yong Liu Ying Han 《科学通报:英文版》 SCIE EI CSCD 2019年第14期998-1010,共13页
Several monocentric studies have noted alterations in spontaneous brain activity in Alzheimer's disease (AD), although there is no consensus on the altered amplitude of low-frequency fluctuations in AD patients. T... Several monocentric studies have noted alterations in spontaneous brain activity in Alzheimer's disease (AD), although there is no consensus on the altered amplitude of low-frequency fluctuations in AD patients. The main aim of the present study was to identify a reliable and reproducible abnormal brain activity pattern in AD. The amplitude of local brain activity (AM), which can provide fast mapping of spontaneous brain activity across the whole brain, was evaluated based on multisite rs-fMRI data for 688 subjects (215 normal controls (NCs), 221 amnestic mild cognitive impairment (aMCI) 252 AD). Two-sample t-tests were used to detect group differences between AD patients and NCs from the same site. Differences in the AM maps were statistically analyzed via the Stouffer's meta-analysis. Consistent regions of lower spontaneous brain activity in the default mode network and increased activity in the bilateral hippocampus/parahippocampus, thalamus, caudate nucleus, orbital part of the middle frontal gyrus and left fusiform were observed in the AD patients compared with those in NCs. Significant correlations (P?<?0.05, Bonferroni corrected) between the normalized amplitude index and Mini-Mental State Examination scores were found in the identified brain regions, which indicates that the altered brain activity was associated with cognitive decline in the patients. Multivariate analysis and leave-one-site-out cross-validation led to a 78.49% prediction accuracy for single-patient classification. The altered activity patterns of the identified brain regions were largely correlated with the FDG-PET results from another independent study. These results emphasized the impaired brain activity to provide a robust and reproducible imaging signature of AD. 展开更多
关键词 Brain SPONTANEOUS activity Multisite Biomarkers Leave-one-site-out cross-validation Alzheimer's disease
不同时长睡眠干扰对SD大鼠步态及认知能力的影响 认领 被引量:1
11
作者 卜兰兰 金剑 +1 位作者 李思迪 刘新民 《中国比较医学杂志》 CAS 北大核心 2019年第11期65-70,115共7页
目的 研究不同时长睡眠干扰对SD大鼠步态及认知能力的影响。方法 将64只SD大鼠随机分为睡眠干扰2 d、5 d、10 d、15 d模型组及相应对照组,共8组,每组8只。用滚筒法对大鼠进行睡眠干扰后,到达相应时间点时先进行自主活动检测,再用步态分... 目的 研究不同时长睡眠干扰对SD大鼠步态及认知能力的影响。方法 将64只SD大鼠随机分为睡眠干扰2 d、5 d、10 d、15 d模型组及相应对照组,共8组,每组8只。用滚筒法对大鼠进行睡眠干扰后,到达相应时间点时先进行自主活动检测,再用步态分析仪评估睡眠干扰对SD大鼠步态的影响,最后用水迷宫检测其学习记忆能力。结果 自主活动显示,与相应对照组比较,5 d模型组总路程、平均速度、运动总时间、边缘区运动时间明显减少(P<0. 05),静息总时间明显增加(P<0. 05);10 d模型组总路程、平均速度减少(P<0. 05),运动总时间、边缘区运动时间显著减少(P<0. 01),静息总时间显著增加(P<0. 01)。步态结果显示,与相应对照组比较,5 d模型组平均步行周期明显缩短(P<0. 05);2 d、15 d模型组步行速度明显下降、双支撑时相-左前-右后明显缩短(P<0. 05),双支撑时相-左后-右前显著缩短、三支撑时相-右后-左后-右前显著增加(P<0. 01);2 d、5 d、15 d模型组右后步幅明显缩小(P<0. 05)。水迷宫结果显示,与相应对照组比较,5 d模型组定位航行第1~4天潜伏期明显增加(P<0. 05),10 d模型组定位航行第2、4天潜伏期明显增加(P<0. 05),15 d模型组定位航行第1~5天潜伏期明显增加(P<0. 05),其中5 d模型组定位航行第1天潜伏期显著增加(P<0. 01),15 d模型组定位航行第1~3天潜伏期显著增加(P<0. 01)。结论 不同时长睡眠干扰对SD大鼠步态、自主活动、空间定位学习能力有不同程度的影响。步态检测相比自主活动和水迷宫检测,是评价滚筒法睡眠干扰模型更为敏感的方法。 展开更多
关键词 睡眠干扰 滚筒 步态 自主活动 水迷宫 大鼠
在线阅读 下载PDF
Time-course pattern of neuronal loss and gliosis in gerbil hippocampi following mild, severe, or lethal transient global cerebral ischemia 认领
12
作者 Tae-Kyeong Lee Hyunjung Kim +9 位作者 Minah Song Jae-Chul Lee Joon Ha Park Ji Hyeon Ahn Go Eun Yang Hyeyoung Kim Taek Geun Ohk Myoung Cheol Shin Jun Hwi Cho Moo-Ho Won 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第8期1394-1403,共10页
Transient ischemia in the whole brain leads to neuronal loss/death in vulnerable brain regions. The striatum, neocortex and hippocampus selectively loose specific neurons after transient ischemia. Just 5 minutes of tr... Transient ischemia in the whole brain leads to neuronal loss/death in vulnerable brain regions. The striatum, neocortex and hippocampus selectively loose specific neurons after transient ischemia. Just 5 minutes of transient ischemia can cause pyramidal neuronal death in the hippocampal cornu ammonis (CA) 1 field at 4 days after transient ischemia. In this study, we investigated the effects of 5-minute (mild), 15-minute (severe), and 20-minute (lethal) transient ischemia by bilateral common carotid artery occlusion (BCCAO) on behavioral change and neuronal death and gliosis (astrocytosis and microgliosis) in gerbil hippocampal subregions (CA1-3 region and dentate gyrus). We performed spontaneous motor activity test to evaluate gerbil locomotor activity, cresyl violet staining to detect cellular distribution, neuronal nuclei immunohistochemistry to detect neuronal distribution, and Fluoro-Jade B histofluorescence to evaluate neuronal death. We also conducted immunohistochemical staining for glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1 (Ibal) to evaluate astrocytosis and microgliosis, respectively. Animals subjected to 20-minute BCCAO died in at least 2 days. BCCAO for 15 minutes led to pyramidal cell death in hippocampal CA1-3 region 2 days later and granule cell death in hippocampal de匚tate gyrus 5 days later. Similar results were not found in animals subjected to 5-minute BCCAO. Gliosis was much more rapidly and severely progressed in animals subjected to 15-minute BCCAO than in those subjected to 5- minute BCCAO. Our results indicate that neuronal loss in the hippocampal formation following transient ischemia is significantly different according to regions and severity of transient ischemia. The experimental protocol was approved by Institutional Animal Care and Use Committee (AICUC) of Kangwon National University (approval No. KW-180124-1) on May 22, 2018. 展开更多
关键词 TRANSIENT global brain ischemia delayed neuronal death GLIAL activation ischemic duration hippocampus spontaneous motor activity Mongolian GERBIL histology neural regeneration
在线阅读 下载PDF
SD大鼠与FH/Wjd大鼠神经行为学差异比较 认领 被引量:1
13
作者 熊庭旺 贺希 +3 位作者 魏胜焦 刘波 吴芹 石京山 《遵义医学院学报》 2018年第6期663-668,共6页
目的以SD大鼠为对照,探究FH/Wjd大鼠学习记忆、自主活动、疲劳耐力是否满足抑郁模型的部分标准。方法Morris水迷宫、Y迷宫检测3、6及9月龄SD大鼠、FH/Wjd大鼠学习记忆能力;Y迷宫检测3、6及9月龄SD大鼠、FH/Wjd大鼠自主活动能力、探索能... 目的以SD大鼠为对照,探究FH/Wjd大鼠学习记忆、自主活动、疲劳耐力是否满足抑郁模型的部分标准。方法Morris水迷宫、Y迷宫检测3、6及9月龄SD大鼠、FH/Wjd大鼠学习记忆能力;Y迷宫检测3、6及9月龄SD大鼠、FH/Wjd大鼠自主活动能力、探索能力;转棒实验观察3、6及9月龄SD大鼠、FH/Wjd大鼠疲劳耐受力。结果3、6和9月龄FH/Wjd大鼠水迷宫逃避潜伏期均高于同月龄SD大鼠,目标象限停留时间百分比、目标象限运动距离百分比、目标象限进入次数百分比均低于同月龄的SD大鼠;3、6及9月龄FH/Wjd大鼠Y迷宫入臂总次数、活动速度高于同月龄的SD大鼠;3、6及9月龄FH/Wjd大鼠转棒上停留时间低于同月龄的SD大鼠。结论FH/Wjd大鼠学习记忆能力、疲劳耐力低于同月龄SD大鼠;探索能力、活动能力高于SD大鼠,FH/Wjd大鼠符合抑郁模型的部分标准。 展开更多
关键词 SD大鼠 FH/Wjd大鼠 抑郁 学习记忆 自主活动 疲劳
在线阅读 免费下载
二氢杨梅素改善抑郁样行为及应激诱导认知障碍的作用 认领 被引量:1
14
作者 于旭东 曲浩天 +2 位作者 朱晓峰 肖志勇 田绍文 《华西药学杂志》 CAS CSCD 2018年第6期585-589,共5页
目的研究二氢杨梅素(DHM)对抑郁样行为及学习记忆的影响。方法将小鼠随机分成无应激+溶媒组(NS+Veh)、无应激+DHM组(NS+DHM)、应激+溶媒组(CRS+Veh)以及应激+DHM组(CRS+DHM),应激组小鼠经历10 d应激,DHM组小鼠在应激后5 d注射DHM,应激... 目的研究二氢杨梅素(DHM)对抑郁样行为及学习记忆的影响。方法将小鼠随机分成无应激+溶媒组(NS+Veh)、无应激+DHM组(NS+DHM)、应激+溶媒组(CRS+Veh)以及应激+DHM组(CRS+DHM),应激组小鼠经历10 d应激,DHM组小鼠在应激后5 d注射DHM,应激结束后所有小鼠依次进行旷场、新颖物体识别、Y迷宫、悬尾、强迫游泳等实验测试,观察DHM对小鼠的自发活动、学习记忆及抑郁样行为的作用。将WKY大鼠随机分为两组Veh组和DHM组,一部分大鼠(每组8只)依次进行旷场和FST测试,另一部分(每组4只)断头取脑,采用Western blot法检测海马内脑源性神经营养因子(BDNF)的水平。结果 CRS+Veh组小鼠在Y迷宫和新颖物体识别测试中的学习记忆能力显著低于NS+Veh组,CRS+DHM组小鼠在Y迷宫和新颖物体识别测试中的学习记忆能力显著高于CRS+Veh组,而与NS+Veh组比较,NS+DHM组在Y迷宫和新颖物体识别测试中的学习记忆能力无显著性差异; CRS+Veh组小鼠在悬尾和强迫游泳测试中的不动时间显著高于NS+Veh组,CRS+DHM组小鼠在悬尾和强迫游泳测试中的不动时间显著低于CRS+Veh组,同时,与NS+Veh组比较,NS+DHM组的悬尾和强迫游泳测试中的不动时间显著降低。在WKY大鼠中,DHM组在强迫游泳测试中的不动时间显著低于Veh组;在旷场测试中两组大鼠穿越的格子数无显著性差异。结论 DHM改善应激诱导小鼠记忆损伤,但对正常小鼠学习记忆无影响; DHM可改善正常小鼠、慢性应激小鼠、WKY大鼠的抑郁样行为以及上调WKY大鼠BDNF的表达。 展开更多
关键词 二氢杨梅素 认知功能障碍 自发活动 学习记忆 抑郁 应激 脑源性神经营养因子 旷场实验 WESTERNBLOT法
天香胶囊对运动病模型大鼠行为学的影响及其机制 认领
15
作者 张炜悦 李卫红 +4 位作者 胡艳红 张凡 张蔚 戴宁 李峰 《海军医学杂志》 2018年第1期25-28,共4页
探讨天香胶囊对运动病(motion sickness,MS)模型大鼠行为学的影响及其机理。将60只SPF级雄性SD大鼠随机分为6组:空白对照组、模型对照组、阳性对照组、天香胶囊低、中、高剂量组,每组10只,各组大鼠在造模前1 h给药,采用旋转刺激装置持... 探讨天香胶囊对运动病(motion sickness,MS)模型大鼠行为学的影响及其机理。将60只SPF级雄性SD大鼠随机分为6组:空白对照组、模型对照组、阳性对照组、天香胶囊低、中、高剂量组,每组10只,各组大鼠在造模前1 h给药,采用旋转刺激装置持续刺激2 h,诱发大鼠运动病模型,采用自发活动实验观察大鼠行为学变化、ELISA和放免法检测前庭核乙酰胆碱(ACh)含量和血浆促肾上腺皮质激素(ACTH)、β-内啡肽(β-EP)水平。天香胶囊各剂量组均可增加运动病模型大鼠的自发活动总距离和活动时间,减低血浆ACTH[空白对照组(24.48±2.28)ng/L,天香低剂量组(30.56±4.26)ng/L]和β-EP[空白对照组(141.27±12.11)ng/L,天香低剂量组(164.03±23.60)ng/L]含量以及前庭核ACh[空白对照组(58.87±6.03)ng/L,天香低剂量组(67.78±10.34)ng/L]含量,且显示出一定的剂量依赖关系。天香胶囊可显著改善运动病模型大鼠的自发活动,其机制可能与缓解中枢神经系统兴奋性降低,降低血浆ACTH、β-EP含量以及前庭核ACh含量有关,提示中药复方天香胶囊对运动病有一定防治作用,其高剂量对模型大鼠作用效果显著,为最佳剂量。 展开更多
关键词 天香胶囊 运动病 自发活动 应激反应
在线阅读 下载PDF
桑白皮总黄酮对小鼠睡眠功能和自发活动影响的研究 认领 被引量:2
16
作者 饶晓兵 邱桑 +1 位作者 庄韬 周林 《中国中医药现代远程教育》 2017年第15期145-146,共2页
目的研究桑白皮总黄酮对小鼠睡眠功能和自发活动的影响。方法 1)催眠:昆明种小鼠随机分为生理盐水对照组、1.0 mg/kg桑白皮总黄酮组、2.0 mg/kg桑白皮总黄酮组、4.0 mg/kg桑白皮总黄酮组(下同),观察给药后桑白皮总黄酮对各组小鼠阈... 目的研究桑白皮总黄酮对小鼠睡眠功能和自发活动的影响。方法 1)催眠:昆明种小鼠随机分为生理盐水对照组、1.0 mg/kg桑白皮总黄酮组、2.0 mg/kg桑白皮总黄酮组、4.0 mg/kg桑白皮总黄酮组(下同),观察给药后桑白皮总黄酮对各组小鼠阈上剂量和阈下剂量戊巴比妥钠睡眠时间和入睡时间(睡眠作用)的影响;2)自发活动:采用抖笼法测定桑白皮总黄酮对小鼠自发活动(镇静作用)的影响。结果 1)各剂量的桑白皮总黄酮均能显著缩短阈上剂量和阈下剂量戊巴比妥钠的入睡时间和显著延长阈上剂量和阈下剂量戊巴比妥钠的睡眠时间;2)各剂量的桑白皮总黄酮对小鼠自发活动均具有明显抑制作用,均能明显减少中波和大波的活动。结论桑白皮总黄酮具有明显的抑制小鼠自发活动和镇静催眠作用。 展开更多
关键词 桑白皮总黄酮 催眠 自发活动 动物实验
在线阅读 免费下载
基于静系统功能磁共振的躯体化障碍默认网络的自发神经活动研究 认领 被引量:2
17
作者 万红艳 鲁际 +1 位作者 金晶 雷莉 《国际精神病学杂志》 2017年第4期616-618,634共4页
目的探讨运用静系统功能磁共振(rs-f MRI)方法研究躯体化障碍默认网络的自发神经活动的机制。方法选取2014年6月至2016年8月于我院就诊的躯体化障碍患者共80例(观察组),取80例名年龄、性别、教育程度均匹配的健康体检者作为对照组... 目的探讨运用静系统功能磁共振(rs-f MRI)方法研究躯体化障碍默认网络的自发神经活动的机制。方法选取2014年6月至2016年8月于我院就诊的躯体化障碍患者共80例(观察组),取80例名年龄、性别、教育程度均匹配的健康体检者作为对照组。所有患者均使用HAMD量表、和SCL-90量表的躯体化障碍因子分量表评估,并进行rs-f MRI扫描分析。结果其中观察组患者HAMD、HAMA和躯体化障碍因子评分显著高于对照组(P〈0.01),观察组较正常对照组f ALFF增高脑区为位于DMN前部的双内侧前额叶(MPFC)上部(Voxels=66,t=4.43),减弱脑区为位于DMN后部的左模前叶(PCu)(Voxels=74,t=-4.66),观察组双MPFC上部的簇的Z值与SCL-90的躯体化障碍因子分数呈正相关(P〈0.01,cluster size〉52 voxel),与年龄、教育年限、病程、HAMD、HAMA评分无显著相关性。结论躯体化障碍患者DMN神经活动存在分离模式,MPFC的f ALFF值可作为判断躯体化症状严重程度的指标。 展开更多
关键词 静息态功能核磁共振 躯体化障碍 自发神经活动 严重程度
臭牡丹乙醇提取物对小鼠睡眠功能和自发活动的影响 认领
18
作者 黄卫华 钟文敏 +2 位作者 王德胜 曾雪亮 李蓓 《中国医院用药评价与分析》 2017年第10期1313-1314,1318共3页
目的:探讨臭牡丹乙醇提取物对小鼠睡眠功能和自发活动的影响。方法:(1)臭牡丹的提取。采用75%乙醇浸渍臭牡丹48 h,制得质量浓度为1.0 g/ml的臭牡丹乙醇提取物。(2)小鼠自发活动。取小鼠30只,随机分为对照组、观察1组和观察2组,每... 目的:探讨臭牡丹乙醇提取物对小鼠睡眠功能和自发活动的影响。方法:(1)臭牡丹的提取。采用75%乙醇浸渍臭牡丹48 h,制得质量浓度为1.0 g/ml的臭牡丹乙醇提取物。(2)小鼠自发活动。取小鼠30只,随机分为对照组、观察1组和观察2组,每组10只,采用抖笼法测定小鼠自发活动情况。(3)睡眠功能。采用阈上剂量、阈下剂量的戊巴比妥钠致小鼠睡眠的方法,观察臭牡丹乙醇提取物对小鼠睡眠时间的影响。结果:给药后,观察1、2组小鼠15 s内大波、中波出现个数明显少于对照组,差异均有统计学意义(P〈0.05)。给予阈上剂量戊巴比妥钠时,观察1、2组小鼠的睡眠持续时间均明显长于对照组,入睡时间明显短于对照组,差异均有统计学意义(P〈0.05)。给予阈下剂量戊巴比妥钠时,对照组无小鼠入睡,观察1、2组小鼠入睡数量分别为4、7只。结论:臭牡丹乙醇提取物可明显抑制小鼠自发活动,并具有镇静催眠的作用。 展开更多
关键词 臭牡丹 乙醇提取物 镇静催眠 自发活动 小鼠
在线阅读 下载PDF
辅酶Q10中枢神经系统的安全性评价 认领 被引量:1
19
作者 杨宏博 孙庆弟 +6 位作者 谭莹 刘慧浪 廖伟 史新辉 苏敏 包广雷 王京昆 《药物评价研究》 CAS 2017年第7期926-930,共5页
目的考察大剂量ig辅酶Q10对小鼠中枢神经系统的影响,为临床安全性评价提供实验依据。方法将小鼠随机分为溶媒对照组、紫苏油对照组、阳性对照组(氯丙嗪或地西泮)和辅酶Q10低、中、高剂量组(1.5、3.0和6.0 g/kg,相当于临床等效剂量的7... 目的考察大剂量ig辅酶Q10对小鼠中枢神经系统的影响,为临床安全性评价提供实验依据。方法将小鼠随机分为溶媒对照组、紫苏油对照组、阳性对照组(氯丙嗪或地西泮)和辅酶Q10低、中、高剂量组(1.5、3.0和6.0 g/kg,相当于临床等效剂量的75、150和300倍),每组12只,雌雄各半,40 m L/kg单次ig给药。直接观察小鼠一般行为活动;转棒法观察小鼠运动协调能力;Anymaze动物行为学视频分析系统观察小鼠自发活动和与阈下剂量戊巴比妥钠的协同作用。结果与溶媒对照组、紫苏油对照组比较,辅酶Q10 3个剂量组对小鼠一般行为活动、自发活动、运动协调能力和与阈下剂量戊巴比妥钠的协同作用均无显著差异。结论大剂量ig辅酶Q10对小鼠中枢神经系统未见明显毒性作用。 展开更多
关键词 辅酶Q10 中枢神经系统 毒性 小鼠 安全性评价 运动协调能力 自发活动 协同作用
聚乙二醇修饰重组人血管内皮抑制素对BALB/c小鼠自主活动和协同睡眠的影响 认领
20
作者 耿兴超 吴非 +2 位作者 王超 彭茜 李波 《药物评价研究》 CAS 2016年第4期585-588,共4页
目的根据新药申报要求,研究聚乙二醇修饰重组人血管内皮抑制素(M2ES)对小鼠自主活动和协同睡眠的影响,以确定其可能关系到人安全性的非期望药理作用。方法 120只BALB/c小鼠,随机分为4组:对照组和M2ES低、中、高剂量组,分别单次尾iv... 目的根据新药申报要求,研究聚乙二醇修饰重组人血管内皮抑制素(M2ES)对小鼠自主活动和协同睡眠的影响,以确定其可能关系到人安全性的非期望药理作用。方法 120只BALB/c小鼠,随机分为4组:对照组和M2ES低、中、高剂量组,分别单次尾iv氯化钠注射液和6、12、24 mg/kg的M2ES注射液。测定给药前和给药后15、60、120、240、360 min小鼠5 min内的自发活动次数,以及对阈下剂量戊巴比妥钠致小鼠睡眠的影响。结果与对照组比较,M2ES低、中、高剂量组在给药前后各时间点小鼠的自主活动次数以及睡眠发生率均差异不显著。结论 M2ES在24 mg/kg及其以下剂量下对小鼠自主活动无显著性影响,与阈下催眠剂量的戊巴比妥钠无明显协同催眠作用。 展开更多
关键词 聚乙二醇修饰重组人血管内皮抑制素 BALB/C小鼠 自主活动 协同睡眠 戊巴比妥钠
上一页 1 2 10 下一页 到第
使用帮助 返回顶部 意见反馈