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Effects of neural stem cell transplantation on the motor function of rats with contusion spinal cord injuries:a meta-analysis 预览
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作者 Kai Qian Tuo-Ye Xu +7 位作者 Xi Wang Tao Ma Kai-Xin Zhang Kun Yang Teng-Da Qian Jing Shi Li-Xin Li Zheng Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期748-758,共11页
Objective:To judge the efficacies of neural stem cell(NSC)transplantation on functional recovery following contusion spinal cord injuries(SCIs).Data sources:Studies in which NSCs were transplanted into a clinically re... Objective:To judge the efficacies of neural stem cell(NSC)transplantation on functional recovery following contusion spinal cord injuries(SCIs).Data sources:Studies in which NSCs were transplanted into a clinically relevant,standardized rat model of contusion SCI were identified by searching the PubMed,Embase and Cochrane databases,and the extracted data were analyzed by Stata 14.0.Data selection:Inclusion criteria were that NSCs were used in in vivo animal studies to treat contusion SCIs and that behavioral assessment of locomotor functional recovery was performed using the Basso,Beattie,and Bresnahan lo-comotor rating scale.Exclusion criteria included a follow-up of less than 4 weeks and the lack of control groups.Outcome measures:The restoration of motor function was assessed by the Basso,Beattie,and Bresnahan locomotor rating scale.Results:We identified 1756 non-duplicated papers by searching the aforementioned electronic databases,and 30 full-text articles met the inclusion criteria.A total of 37 studies reported in the 30 articles were included in the meta-analysis.The meta-analysis results showed that transplanted NSCs could improve the motor function recovery of rats following contusion SCIs,to a moderate extent(pooled standardized mean difference(SMD)=0.73;95%confidence interval(CI):0.47–1.00;P<0.001).NSCs obtained from different donor species(rat:SMD=0.74;95%CI:0.36–1.13;human:SMD=0.78;95%CI:0.31–1.25),at different donor ages(fetal:SMD=0.67;95%CI:0.43–0.92;adult:SMD=0.86;95%CI:0.50–1.22)and from different origins(brain-derived:SMD=0.59;95%CI:0.27–0.91;spinal cord-derived:SMD=0.51;95%CI:0.22–0.79)had similar efficacies on improved functional recovery;however,adult induced pluripotent stem cell-derived NSCs showed no significant efficacies.Furthermore,the use of higher doses of transplanted NSCs or the administration of immunosuppressive agents did not promote better locomotor function recovery(SMD=0.45;95%CI:0.21–0.70).However,shorter periods between the contusion induction and the NSC tr 展开更多
关键词 Basso Beattie and Bresnahan locomotor rating scale CELL TRANSPLANTATION META-ANALYSIS motor functional recovery NEURAL regeneration NEURAL stem CELL NEURAL stem CELL TRANSPLANTATION rat model SPINAL CONTUSION SPINAL cord injury
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Hepatocellular carcinoma recurrence in living and deceased donor liver transplantation:a systematic review and meta-analysis
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作者 Hai-Ming Zhang Yue-Xian Shi +1 位作者 Li-Ying Sun Zhi-Jun Zhu 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第13期1599-1609,共11页
Background:Although a number of technical problems and donor safety issues associated with living donor liver transplantation(LDLT)have been resolved,some initial clinical studies showed an increased risk of hepatocel... Background:Although a number of technical problems and donor safety issues associated with living donor liver transplantation(LDLT)have been resolved,some initial clinical studies showed an increased risk of hepatocellular car cinoma(HCC)recurrence in LDLT.This meta-analysis was conducted to assess differences in tumor recurrence between LDLT and deceased donor liver transplantation(DDLT).Methods:After systematic retrievals of studies about LDLT and DDLT for HCC,articles were selected with a rationale of emphasizing inter-group comparability.Results from multivariate analyses were combined and discussed together with univariate analyses.In subgroup analysis,the impact of organ allocation policy was taken into consideration.Results:Seven articles were included in the meta-analysis.Overall,a salient result that emerged from the seven studies was a significant increased risk of HCC recurrence in the LDLT group than in the DDLT group(P=0.01).The most significant increase in hazard ratio was found in studies where organs tended to be allocated to non-tumor patients.Conclusions:An increased risk for HCC recurrence in LDLT as compared with DDLT patients was found.The relatively shorter preoperative observation windows in LDLT may lead to fewer cases of HCC with invasive features being screened out,which may provide a possible explanation for the high rates of HCC recurrence. 展开更多
关键词 LIVING DONOR LIVER TRANSPLANTATION Deceased DONOR LIVER TRANSPLANTATION Hepatocellular car cinoma META-ANALYSIS
Loco-regional hepatocellular carcinoma treatment services as a bridge to liver transplantation 预览
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作者 Sophia Schmitz Georg Lurje +5 位作者 Florian Ulmer Anne Andert Philipp Bruners Maximilian Schulze-Hagen Ulf Neumann Wenzel Schoening 《国际肝胆胰疾病杂志:英文版》 SCIE CAS CSCD 2019年第3期228-236,共9页
Background: Liver transplantation remains the main curative treatment option for hepatocellular carcinoma(HCC) patients. In the Eurotransplant area Milan criteria are used to assign priority extra points(exceptional M... Background: Liver transplantation remains the main curative treatment option for hepatocellular carcinoma(HCC) patients. In the Eurotransplant area Milan criteria are used to assign priority extra points(exceptional MELD, ex MELD) for patients on the waiting list. To prevent patients from tumor progression, loco-regional(neoadjuvant) treatment(LRT) is used. For patients unlikely to timely receive an organ via primary allocation,“extended critera donor(ECD) organs” are used. The present study aimed to investigate the survival after LT with a strategy of minimizing waiting list dropouts by using LRT for bridging and transplanting ECD organs if possible and necessary. Methods: Between October 2010 and May 2015, 50 liver transplants for HCC were included in this retrospective study. Of those, 42(84%) met the Milan criteria according to the preoperative radiological examination. Forty-one patients(82%) received LRT. The waiting time was analyzed according to LRT. Kaplan-Meier curves with log-rank statistics were used for survival analyses. Results: One-and five-year overall survival within Milan criteria was 94.3% and 83.7% compared with 91.7% and 67.9% beyond Milan criteria, though statistical significance was not reached( P = 0.487). LRT had no impact on overall survival( P = 0.629). Median waiting time was shorter if no LRT was performed(4.6 months vs. 1.5 months, P = 0.006) and there were no cases of waiting list dropouts. Using ECD organs had no impact on overall survival( P = 0.663). Conclusions: Patients with an expected waiting time to transplantation of > 6 months could be successfully treated with LRT as a bridge to transplant. Overall and disease-free survival for patients within and beyond Milan criteria was comparable and the use of ECD organs in this cohort of HCC patients proved to be a safe option. 展开更多
关键词 Hepatocellular carcinoma LIVER TRANSPLANTATION THERAPEUTIC EMBOLIZATION LIVER NEOPLASMS ORGAN TRANSPLANTATION
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Comparison analysis between haplo identical stem cell transplantation and matched sibling donor stem cell transplantation for high-risk acute myeloid leukemia in first complete remission
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作者 Yang Liu Xiaojun Huang +6 位作者 Qian Fei Lanping Xu Xiaohui Zhang Kaiyan Liu Yuhong Chen Huan Chen Yu Wang 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第5期691-697,共7页
In order to compare the effect between haploidentical(HID) stem cell transplantation(HSCT) and matched sibling donor(MSD)stem cell transplantation for high-risk acute myeloid leukemia(AML) in first complete remission ... In order to compare the effect between haploidentical(HID) stem cell transplantation(HSCT) and matched sibling donor(MSD)stem cell transplantation for high-risk acute myeloid leukemia(AML) in first complete remission status(CR1), we retrospectively studied 170 cases who received stem cell transplantation from Jan 2008 to Jul 2015 in Peking University People’s Hospital. We divided all cases into MSD group(43 cases) and HID(127 cases) group. Patients in HID and MSD group displayed similar baseline characteristics except for age distribution. There were no statistic differences for overall survival(OS), cumulative incidence of relapse, leukemia free survival(LFS), transplantation related mortality(TRM) between HID and MSD group. The 3-year OS, LFS for all patients was 63.9% and 59.7% respectively. Multivariate analysis showed that grade III-IV acute graft versus host disease(aGVHD) was an independent risk factor for treatment related mortality(HR=8.134, 95% CI:3.210–20.611, P<0.001), monosomy/complex chromosomal karyotype and white blood cell count more than 50×109 L-1 were two independent factors for relapse(HR=1.533, 95% CI: 1.040–2.260, P=0.031)(HR=1.004, 95% CI: 1.001–1.008, P=0.015).Grade III-IV aGVHD was an independent factor for mortality(HR=3.184, 95% CI: 1.718–5.902, P<0.001). These results demonstrated some risk factors for high-risk AML leukemia transplantation and indicated for AML patients in CR1 status, haplo stem cell transplantation could have the same therapeutic effect as MSD transplantation. 展开更多
关键词 high risk AML haplo stem cell TRANSPLANTATION matched SIBLING DONOR TRANSPLANTATION complex/monosomy chromosomal KARYOTYPE
APC重建技术联合3D打印个性化非限制性全膝置换在胫骨上段恶性肿瘤中的应用 预览
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作者 王虹 丁焕文 黄敏强 《中国骨科临床与基础研究杂志》 2019年第1期5-14,共10页
目的探究同种异体骨-假体复合物(APC)重建技术联合3D打印个性化非限制性全膝置换(TKR)治疗胫骨上段恶性肿瘤的临床疗效。方法回顾性分析南部战区总医院2005年1月至2015年1月收治的25例胫骨上段恶性肿瘤患者的临床资料。根据术式不同分... 目的探究同种异体骨-假体复合物(APC)重建技术联合3D打印个性化非限制性全膝置换(TKR)治疗胫骨上段恶性肿瘤的临床疗效。方法回顾性分析南部战区总医院2005年1月至2015年1月收治的25例胫骨上段恶性肿瘤患者的临床资料。根据术式不同分为个性化手术组和常规手术组,个性化手术组12例,采用APC重建技术联合3D打印个性化非限制性TKR治疗;常规手术组13例,采用常规手术+铰链式假体治疗。比较两组患者瘤段切除长度、术中出血量、手术时间以及术后双下肢长度差、力线偏移和并发症发生情况,记录两组随访期间肿瘤复发率、膝关节活动度以及骨与软组织肿瘤学会(MSTS)膝关节功能评分。结果两组患者瘤段切除长度、术中出血量、并发症发生率及肿瘤复发率比较,差异无统计学意义(P>0.05)。个性化手术组手术时间、术后肢体长度差和力线偏移度明显少于常规手术组,术后1、3年膝关节主动屈曲度及MSTS膝关节功能评分明显高于常规手术组(P<0.05)。结论与常规手术联合铰链式假体相比,APC重建技术联合个性化非限制性TKR治疗胫骨上段恶性肿瘤解剖匹配精度高,节省手术时间,能提供良好的术后功能,临床效果满意。 展开更多
关键词 胫骨 骨肿瘤 膝关节 保肢手术 移植 同种 骨移植 假体和植入物 假体植入 关节成形术 置换 3D打印 图像处理 计算机辅助 外科手术 计算机辅助
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Pancreatic transplantation:Brief review of the current evidence 预览
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作者 Ahmed Aref Tariq Zayan +2 位作者 Ravi Pararajasingam Ajay Sharma Ahmed Halawa 《世界移植杂志》 2019年第4期81-93,共13页
Kidney transplantation is the treatment of choice for management of end-stage renal disease.However,in diabetic patients,the underlying metabolic disturbance will persist and even may get worse after isolated kidney t... Kidney transplantation is the treatment of choice for management of end-stage renal disease.However,in diabetic patients,the underlying metabolic disturbance will persist and even may get worse after isolated kidney transplantation.Pancreatic transplantation in humans was first introduced in 1966.The initial outcome was disappointing.However,this was changed after the improvement of surgical techniques together with better patient selection and the availability of potent and better-tolerated immune-suppression like cyclosporine and induction antibodies.Combined kidney and pancreas transplantation will not only solve the problem of organ failure,but it will also stabilise or even reverse the metabolic complications of diabetes.Combined kidney and pancreas transplantation have the best long term outcome in diabetic cases with renal failure.Nevertheless,at the cost of an initial increase in morbidity and risk of mortality.Other transplantation options include pancreas after kidney transplantation and islet cell transplantation.We aim by this work to explore various options which can be offered to a diabetic patient with advanced chronic kidney disease.Our work will provide a simplified,yet up-to-date information regarding the different management options for those diabetic chronic kidney failure patients. 展开更多
关键词 Combined KIDNEY pancrease TRANSPLANTATION RENAL TRANSPLANTATION DIABETIC KIDNEY disease DIABETES MELLITUS
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The role of graft reperfusion sequence in the development of non-anastomotic biliary strictures following orthotopic liver transplantation:A meta-analysis 预览
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作者 Mohamed Bekheit Matteo Catanzano +4 位作者 Stuart Shand Irfan Ahmed ELSaid ELKayal Gihan Mohamed Shehata Adel Zaki 《国际肝胆胰疾病杂志:英文版》 SCIE CAS CSCD 2019年第1期4-11,共8页
Background:Liver transplant is a potential cure for liver failure and hepatic malignancy but there are many techniques which have been described for vascular reconstruction.This study was to compare the prevalence of ... Background:Liver transplant is a potential cure for liver failure and hepatic malignancy but there are many techniques which have been described for vascular reconstruction.This study was to compare the prevalence of non-anastomotic biliary stricture and other surgical complications based on Clavien-Dindo scoring system,in initial portal reperfusion(sequential)versus simultaneous or initial artery reperfusion.Data sources:Meta-analysis of published studies comparing the outcomes of both techniques was carried out.Data search was conducted across the major databases and studies were selected under the guidance of the Cochrane guidelines for systematic reviews and meta-analysis.Results:Seven studies were included to address the primary and the secondary outcomes.No statistical difference was found in the incidence of non-anastomotic biliary strictures(OR=0.40;P=0.14),regardless of reperfusion technique.The pooled estimate of the Clavien-Dindo grading of complications was not significantly different between the techniques,though Clavien-Dindo II complications were higher in the simultaneous or initial artery reperfusion group than the initial portal reperfusion group(OR=2.73;P=0.01).Similarly,there was no difference in the operative time,hospital stay and other outcomes addressed in this report.Conclusions:The available evidence suggests that there is no significant difference demonstrated in the rate of non-anastomotic biliary strictures or other complications,between the two techniques,except for Clavien-Dindo II complications. 展开更多
关键词 LIVER TRANSPLANTATION ORGAN TRANSPLANTATION LIVER surgery REJECTION
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Prognosis of BK polyomavirus nephropathy: 10-year analysis of 133 renal transplant recipients at a single center
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作者 Xu-Tao Chen Shi-Cong Yang +6 位作者 Jun Li Rong-Hai Deng Wen-Fang Chen Jiang Qiu Li-Zhong Chen Chang-Xi Wang Gang Huang 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第4期388-394,共7页
Background: BK virus-associated nephropathy (BKVN) is an important cause of chronic allograft dysfunction. The objective of our study was to evaluate the prognosis of BKVN. Methods: We retrospectively reviewed the dat... Background: BK virus-associated nephropathy (BKVN) is an important cause of chronic allograft dysfunction. The objective of our study was to evaluate the prognosis of BKVN. Methods: We retrospectively reviewed the data of 133 renal transplant recipients with BKVN treated at the First Affiliated Hospital of Sun Yat-Sen University between July 2007 and July 2017. BK viral loads, graft function, and pathologic indexes were compared between initial diagnosis and last follow-up. Results: After a mean follow-up period of 14.4 (range, 0.3–109.6) months after diagnosis of BKVN, BK viruria, and BK viremia become negative in 19.5% and 90.2% of patients, respectively. The mean estimated glomerular filtration rate (eGFR) at last follow- up was lower than at diagnosis of BKVN (18.3 ± 9.2 vs. 32.8 ± 20.6 mL·min^-1·1.73 m^-2, t= 7.426, P < 0.001). Eight (6.0%) patients developed acute rejection after reducing immunosuppression. At last follow-up, the eGFR was significantly lower in patients with subsequent rejection than those without (21.6 ± 9.8 vs. 33.5 ± 20.9 mL·min^-1·1.73 m^-2, t= 3.034, P= 0.011). In 65 repeat biopsies, SV40-T antigen staining remained positive in 40 patients and became negative in the other 20 patients. The eGFR (42.6 ± 14.3 vs. 26.5 ± 12.3 mL·min^-1·1.73 m^-2), urine viral loads (median, 1.3 × 105vs. 1.4 × 107 copies/mL), and plasma viral load (median, 0 vs. 0 copies/mL) were all significantly lower in patients with negative SV40-T antigen staining than those with persistent BK involvement (all, P < 0.05). Five (3.8%) recipients lost their graft at diagnosis of BKVN, and 13 (9.8%) lost their graft during the follow-up period. The 1-, 3-, and 5-year graft survival rates after diagnosis of BKVN were 99.2%, 90.7%, and 85.7%, respectively. Higher pathologic stage correlated with lower allograft survival rate (χ^2= 6.341, P= 0.042). Conclusion: Secondary rejection and persistent histologic infection in BKVN lead to poor prognosis. 展开更多
关键词 Kidney transplantation BK virus PATHOLOGY REJECTION PROGNOSIS
Shoot proliferation and callus regeneration from nodular buds of Drepanostachyum luodianense 预览
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作者 Shuyan Lin Guohua Liu +3 位作者 Tingting Guo Li Zhang Shuguang Wang Yulong Ding 《林业研究:英文版》 CAS CSCD 2019年第6期1997-2005,共9页
This research reports on an efficient shoot proliferation and callus regeneration system for bamboo.Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense(Yi et R. S. Wang) Keng f.were us... This research reports on an efficient shoot proliferation and callus regeneration system for bamboo.Young, semi-lignified branches with one lateral bud from Drepanostachyum luodianense(Yi et R. S. Wang) Keng f.were used as explants. Disinfection with 0.1% HgCl2 for 8 min was the optimum treatment and the best medium for bud initiation was Murashige and Skoog(MS) medium containing 3.0 mg L-16-benzyladenine(BA). Multiple shoots were induced from nodal shoot segments on MS medium containing 5.0 mg L-1 BA, 0.5 mg L-1 kinetin(Kin), and 1.0 mg L-1 naphthaleneacetic acid(NAA). The highest frequency of callus formation(65.6%) was on MS medium containing 4.0 mg L-12,4-dichlorophenoxyacetic acid(2, 4-D), 0.5 mg L-1 NAA, and 0.1 mg L-1 thidiazuron(TDZ). The optimum medium for callus proliferation was MS medium with 4 mg L-12,4-D, 0.5 mg L-1 TDZ and 0.5 mg L-1 NAA, and the optimum hormone combination was 4 mg L-1 BA ? 0.5 mg L-1 NAA for callus redifferentiation(up to 85.6%). A 100% rooting was achieved on MS medium supplemented with 2.0 mg L-1 NAA and 0.5 mg L-13-indole butyric acid(IBA). Rooted plantlets were acclimatized in a greenhouse in humus soil ? perlite(1:1) substrate. These micropropagated callus induction and regeneration systems for bamboo will be useful for genetic engineering and multiplication. 展开更多
关键词 MICROPROPAGATION BAMBOO SHOOT proliferation CALLUS regeneration Transplantation
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Soluble Nogo receptor 1 fusion protein protects neural progenitor cells in rats with ischemic stroke 预览
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作者 Hai-Wei He Yue-Lin Zhang +4 位作者 Bao-Qi Yu Gen Ye Wei You Kwok-fai So Xin Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1755-1764,共10页
Soluble Nogo66 receptor-Fc protein(sNgR-Fc)enhances axonal regeneration following central nervous system injury.However,the underlying mechanisms remain unclear.In this study,we investigated the effects of sNgR-Fc on ... Soluble Nogo66 receptor-Fc protein(sNgR-Fc)enhances axonal regeneration following central nervous system injury.However,the underlying mechanisms remain unclear.In this study,we investigated the effects of sNgR-Fc on the proliferation and differentiation of neural progenitor cells.The photothrombotic cortical injury model of ischemic stroke was produced in the parietal cortex of Sprague-Dawley rats.The rats with photothrombotic cortical injury were randomized to receive infusion of 400μg/kg sNgR-Fc(sNgR-Fc group)or an equal volume of phosphate-buffered saline(photothrombotic cortical injury group)into the lateral ventricle for 3 days.The effects of sNgR-Fc on the proliferation and differentiation of endogenous neural progenitor cells were examined using BrdU staining.Neurological function was evaluated with the Morris water maze test.To further examine the effects of sNgR-Fc treatment on neural progenitor cells,photothrombotic cortical injury was produced in another group of rats that received transplantation of neural progenitor cells from the hippocampus of embryonic Sprague-Dawley rats.The animals were then given an infusion of phosphate-buffered saline(neural progenitor cells group)or sNgR-Fc(sNgR-Fc+neural progenitor cells group)into the lateral ventricle for 3 days.sNgR-Fc enhanced the proliferation of cultured neural progenitor cells in vitro as well as that of endogenous neural progenitor cells in vivo,compared with phosphate-buffered saline,and it also induced the differentiation of neural progenitor cells into neurons.Compared with the photothrombotic cortical injury group,escape latency in the Morris water maze and neurological severity score were greatly reduced,and distance traveled in the target quadrant was considerably increased in the sNgR-Fc group,indicating a substantial improvement in neurological function.Furthermore,compared with phosphate-buffered saline infusion,sNgR-Fc infusion strikingly improved the survival and differentiation of grafted neural progenitor cells.Our findings show that 展开更多
关键词 NEURAL REGENERATION Nogo-66 RECEPTOR Nogo66 receptor-Fc protein NEURAL PROGENITOR cells proliferation differentiation stroke photothrombotic cortical injury transplantation NEUROLOGICAL function nerve REGENERATION
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Neural stem cell transplantation therapy for brain ischemic stroke:Review and perspectives 预览
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作者 Gui-Long Zhang Zhi-Han Zhu Ye-Zhong Wang 《世界干细胞杂志:英文版(电子版)》 2019年第10期817-830,共14页
Brain ischemic stroke is one of the most common causes of death and disability,currently has no efficient therapeutic strategy in clinic.Due to irreversible functional neurons loss and neural tissue injury,stem cell t... Brain ischemic stroke is one of the most common causes of death and disability,currently has no efficient therapeutic strategy in clinic.Due to irreversible functional neurons loss and neural tissue injury,stem cell transplantation may be the most promising treatment approach.Neural stem cells(NSCs)as the special type of stem cells only exist in the nervous system,can differentiate into neurons,astrocytes,and oligodendrocytes,and have the abilities to compensate insufficient endogenous nerve cells and improve the inflammatory microenvironment of cell survival.In this review,we focused on the important role of NSCs therapy for brain ischemic stroke,mainly introduced the methods of optimizing the therapeutic efficacy of NSC transplantation,such as transfection and overexpression of specific genes,pretreatment of NSCs with inflammatory factors,and co-transplantation with cytokines.Next,we discussed the potential problems of NSC transplantation which seriously limited their rapid clinical transformation and application.Finally,we expected a new research topic in the field of stem cell research.Based on the bystander effect,exosomes derived from NSCs can overcome many of the risks and difficulties associated with cell therapy.Thus,as natural seed resource of nervous system,NSCs-based cell-free treatment is a newly therapy strategy,will play more important role in treating ischemic stroke in the future. 展开更多
关键词 ISCHEMIC STROKE Neural stem cells Transplantation CYTOKINES EXOSOMES
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游离同侧多块小腿腓浅动脉穿支皮瓣修复手部多发创面效果观察
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作者 熊胜 巨积辉 +4 位作者 金光哲 朱从坤 张广亮 唐林峰 周广良 《中华烧伤杂志》 CAS CSCD 北大核心 2019年第9期655-660,共6页
目的探讨游离同侧多块小腿腓浅动脉穿支皮瓣修复手部多发创面的效果。方法2017年11月—2018年12月,笔者单位收治8例患者手部18处创面,其中4例分布于示、中指,2例分布于中、环指,1例分布于示、中、环指,1例分布于中、环、小指,手部缺损... 目的探讨游离同侧多块小腿腓浅动脉穿支皮瓣修复手部多发创面的效果。方法2017年11月—2018年12月,笔者单位收治8例患者手部18处创面,其中4例分布于示、中指,2例分布于中、环指,1例分布于示、中、环指,1例分布于中、环、小指,手部缺损面积为1.5 cm×0.8 cm~4.0 cm×3.0 cm。患者中男4例、女4例,年龄34~62岁。6例患者游离同侧2块小腿腓浅动脉穿支皮瓣修复创面,2例患者游离同侧3块小腿腓浅动脉穿支皮瓣修复创面,术中将皮瓣腓浅动脉及其伴行静脉与受区指动脉及掌侧或背侧皮下静脉端端吻合。皮瓣切取面积2.5 cm×1.2 cm~5.0 cm×4.0 cm。术中均未切取神经,供瓣区均直接缝合。记录皮瓣存活情况,供瓣区愈合后情况,随访观察供受区恢复情况。结果术后皮瓣均存活良好,供瓣区愈合良好,未发生血管危象。术后随访4~12个月,皮瓣色泽、质地优良,弹性、功能良好,外形饱满,恢复保护性感觉。1例患者术后5个月指腹处皮瓣稍显臃肿,两点辨别觉距离为10 mm,行皮瓣修薄整形术。小腿供瓣区无明显瘢痕增生,外形良好,功能无影响。结论采用游离同侧多块小腿腓浅动脉穿支皮瓣修复手部多发创面,具有切取方便、操作简单、对供区影响小、临床效果佳的优点。 展开更多
关键词 外科皮瓣 移植 显微外科手术 穿支 腓浅动脉
轻量级嵌入式实时操作系统MQX Lite构件化工程框架研究 预览
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作者 沈海燕 施连敏 +1 位作者 李冬 仇世祥 《工业控制计算机》 2019年第12期57-58,61,共3页
为了降低嵌入式实时操作系统软件开发难度,提高开发效率,提出了一种复用性高、移植性好、便于任务管理的MQX Lite实时操作系统软件开发构件化工程框架MQXLite-FW,该框架将MQX Lite核心以构件的形式添加到原有无操作系统构件化工程框架NO... 为了降低嵌入式实时操作系统软件开发难度,提高开发效率,提出了一种复用性高、移植性好、便于任务管理的MQX Lite实时操作系统软件开发构件化工程框架MQXLite-FW,该框架将MQX Lite核心以构件的形式添加到原有无操作系统构件化工程框架NOS-FW中,并在Kinetis Design Studio集成开发环境和SD-FSL-KW01开发套件上进行移植与实例测试。结果表明该工程框架布局规范、结构清晰、可读性强、可移植性高。 展开更多
关键词 MQX Lite 工程框架 构件化 移植
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Cerebral toxoplasmosis after allogeneic hematopoietic stem cell transplantation diagnosed by megagenomic analysis
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作者 Kai Shen Ting Liu Jie Ji 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第5期609-610,共2页
To the Editor:A 19-year-old Chinese man, who received haploidentical allogeneic hematopoietic stem cell transplantation (allo-HSCT) for acute T-lymphoblastic leukemia 6 months ago,was evaluated for dysarthria, vomitin... To the Editor:A 19-year-old Chinese man, who received haploidentical allogeneic hematopoietic stem cell transplantation (allo-HSCT) for acute T-lymphoblastic leukemia 6 months ago,was evaluated for dysarthria, vomiting, high fever,and headache. 展开更多
关键词 CEREBRAL TOXOPLASMOSIS ALLOGENEIC HEMATOPOIETIC stem cell TRANSPLANTATION megagenomic analysis
Everyone has a donor:contribution of the Chinese experience to global practice of haploidentical hematopoietic stem cell transplantation
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作者 Meng Lv Yingjun Chang Xiaojun Huang 《医学前沿:英文版》 CAS CSCD 2019年第1期45-56,共12页
Human leukocyte antigen (HLA)-matched donors for hematopoietic stem cell transplantation (HSCT) have long been scarce in China. Haploidentical (haplo) donors are available for the vast majority of patients, but toxici... Human leukocyte antigen (HLA)-matched donors for hematopoietic stem cell transplantation (HSCT) have long been scarce in China. Haploidentical (haplo) donors are available for the vast majority of patients, but toxicity has limited this approach. Three new approaches for haplo-HSCT originated from Italy, China, and USA in 1990 and have been developed to world-renowned system up to now. The Chinese approach have been greatly improved by implementing new individualized conditioning regimens, donor selection based on non-HLA systems, risk-directed strategies for graft-versus-host disease and relapse, and infection management. Haplo-HSCT has exhibited similar efficacy to HLA-matched HSCT and has gradually become the predominant donor source and the first alternative donor choice for allo-HSCT in China. Registry-based analyses and multicenter studies adhering to international standards facilitated the transformation of the unique Chinese experience into an inspiration for the refinement of global practice.This review will focus on how the new era in which "everyone has a donor" will become a reality in China. 展开更多
关键词 HAPLOIDENTICAL HEMATOPOIETIC stem cell transplantation conditioning GRAFT-VERSUS-HOST disease RELAPSE infection DONOR selection
Bone marrow mesenchymal stem cells combined with Sox2 increase the functional recovery in rat with traumatic brain injury
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作者 Qiang Hao Jian Zheng +1 位作者 Yue Hu Hao Wang 《中华神经外科杂志(英文)》 CSCD 2019年第2期85-91,共7页
Background:About 10 million individuals suffer from traumatic brain injury (TBI) each year in the world,which is one of the most serious neurological disorders.The morbidity of TBl is 55.5~6.1/100,000 in China,which t... Background:About 10 million individuals suffer from traumatic brain injury (TBI) each year in the world,which is one of the most serious neurological disorders.The morbidity of TBl is 55.5~6.1/100,000 in China,which takes more costing in the therapy,and the outcome of that is not well.Therefore,we expect to find new methods to treat TBI and improve the outcomes of TBI.In the previous studies reviewed,we found that stem cell transplantation may hold promising potential for modifying motor dysfunction induced by TBI.Methods:Twenty-six adult SD rats were involved in our study.Two adult SD rats were used as donors of bone marrow stem cells (BMSCs),and the other adult SD rats were divided into four groups randomly,which were used to establish the TBI models.BMSCs were transduced with lentivirous-Sox2,and we try to examine the effects of Sox2 on the differentiation of BMSCs.Establishment of rat TBI model:Rats were anesthetized using pentobarbital sodium (at a concentration of 1.5% and a dose of 40 mg/kg) and fixed under the stereotaxic device.A 1.0-cm craniotomy was performed lateral to the sagittal suture.The skullcap was carefully removed,and rats were then subjected to TBI using a controlled cortical injury instrument.A standardized parietal contusion was performed using a 20-mg steel rod with a diameter of 4 mm,which dropped from a height of 30 cm.After injury,the incision was sutured,and rats were carefully observed and nursed.Treatments:Seven days after TBI,rats were divided into four groups and were transplanted with BMSC-Sox2,single BMSC,single Lentivirus-Sox2,and PBS into injured brain,respectively.The motor function was tested using the neurological severity score (NSS).Results:We found that the ectopic expression of Sox2 enhanced BMSCs to differentiate into neurons.Seven days after TBI,the rats were treated with BMSC-Sox2,BMSC,Sox2,and PBS.Results showed that NSS were 3.352 ± 0.398 in the BMSC-Sox2 group,4.013 ± 0.495 in the BMSC group,4.968 ± 0.293 in the Sox2 group,and 6.257 ± 0.361 in the PBS group,su 展开更多
关键词 TRAUMATIC brain injury Bone MARROW stem cell SOX2 gene TRANSPLANTATION
旋髂浅动脉联合缝匠肌蒂髂骨瓣治疗青壮年股骨颈骨折
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作者 左荣跃 陈一勇 +5 位作者 史增元 毛海蛟 尹维刚 林荣 丁杰 刘振新 《中华显微外科杂志》 CSCD 北大核心 2019年第3期254-257,共4页
目的探讨旋髂浅动脉联合缝匠肌蒂髂骨瓣移植治疗青壮年股骨颈骨折的解剖与临床疗效.方法从2012年4月至2017年3月,对50侧经动脉灌注红色乳胶的成人尸体下肢标本,解剖观测旋髂浅动脉、缝匠肌的动脉来源、外径、走行与分布.设计旋髂浅动脉... 目的探讨旋髂浅动脉联合缝匠肌蒂髂骨瓣移植治疗青壮年股骨颈骨折的解剖与临床疗效.方法从2012年4月至2017年3月,对50侧经动脉灌注红色乳胶的成人尸体下肢标本,解剖观测旋髂浅动脉、缝匠肌的动脉来源、外径、走行与分布.设计旋髂浅动脉联合缝匠肌蒂髂骨瓣移植治疗青壮年股骨颈骨折11例,其中男9例,女2例,年龄16~54岁,平均34.5岁.GardenⅢ型骨折4例,Ⅳ型7例.门诊定期随访.结果旋髂浅动脉起源于股动脉,起始处外径(1.2±0.3)mm,起点至髂前上棘(8.8±1.3)cm,其走行至髂前上棘区并发出1~3条分支入髂嵴.缝匠肌起于髂前上棘,近段动脉来源于旋髂浅动脉和股动脉,动脉呈节段性进入缝匠肌并在肌内形成链型吻合营养髂骨瓣.临床应用旋髂浅动脉联合缝匠肌蒂髂骨瓣移植治疗青壮年股骨颈骨折11例,术后切口全部一期愈合.术后随访16~42个月,平均32.3个月,骨折全部愈合,愈合时间3~7个月,平均4.6个月.末次随访时髋关节Harris评分为(93.02±5.33)分:优7例、良4例.所有患者均无骨折不愈合、股骨头坏死等并发症发生.结论旋髂浅动脉联合缝匠肌蒂髂骨瓣移植治疗青壮年股骨颈骨折手术简便,骨瓣血供丰富,有利于促进骨折愈合、减少股骨头坏死的发生,是治疗青壮年股骨颈骨折的一种有效方法. 展开更多
关键词 旋髂浅动脉 缝匠肌 髂骨瓣 股骨颈骨折 移植
Impact of small-for-size liver grafts on medium-term and long-term graft survival in living donor liver transplantation: A meta-analysis 预览
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作者 Ka Wing Ma Kelly Hiu Ching Wong +6 位作者 Albert Chi Yan Chan Tan To Cheung Wing Chiu Dai James Yan Yue Fung Wong Hoi She Chung Mau Lo Kenneth Siu Ho Chok 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第36期5559-5568,共10页
BACKGROUND Small-for-size grafts (SFSGs) in living donor liver transplantation (LDLT) could optimize donor postoperative outcomes and also expand the potential donor pool. Evidence on whether SFSGs would affect medium... BACKGROUND Small-for-size grafts (SFSGs) in living donor liver transplantation (LDLT) could optimize donor postoperative outcomes and also expand the potential donor pool. Evidence on whether SFSGs would affect medium-term and long-term recipient graft survival is lacking. AIM To evaluate the impact of small-for-size liver grafts on medium-term and longterm graft survival in adult to adult LDLT. METHODS A systematic review and meta-analysis were performed by searching eligible studies published before January 24, 2019 on PubMed, EMBASE, and Web of Science databases. The primary outcomes were 3-year and 5-year graft survival. Incidence of small-for-size syndrome and short term mortality were also extracted. RESULTS This meta-analysis is reported according to the guidelines of the PRISMA 2009 Statement. Seven retrospective observational studies with a total of 1821 LDLT recipients were included in the meta-analysis. SFSG is associated with significantly poorer medium-term graft survival. The pooled odds ratio for 3-year graft survival was 1.58 [95% confidence interval 1.10-2.29, P = 0.014]. On the other hand, pooled results of the studies showed that SFSG had no significant discriminatory effect on 5-year graft survival with an odds ratio of 1.31 (95% confidence interval 0.87-1.97, P = 0.199). Furthermore, incidence of small-for-size syndrome detected in recipients of SFSG ranged from 0-11.4% in the included studies. CONCLUSION SFSG is associated with inferior medium-term but not long-term graft survival. Comparable long-term graft survival based on liver graft size shows that smaller grafts could be accepted for LDLT with appropriate flow modulatory measures. Close follow-up for graft function is warranted within 3 years after liver transplantation. 展开更多
关键词 LIVING DONOR liver transplantation Small-for-size GRAFTS Small-for-size syndrome GRAFT survival
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脐带间充质干细胞移植对大鼠脊髓损伤区域神经损伤的修复效果分析 预览
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作者 吴玲玲 潘晓明 +5 位作者 陈浩浩 傅晓艳 王文倩 江文娇 张辉 丁明星 《中国医学工程》 2019年第6期7-8,14共3页
目的探讨脐带间充质干细胞移植对大鼠脊髓损伤区域神经损伤的修复效果。方法选择70只大鼠,雄性,体重220-260g。采用随机分组的方法将其分为研究组和对照组。大鼠均给予脊髓左侧半切,研究组在脊髓半切的基础上行脐带间充质干细胞移植。术... 目的探讨脐带间充质干细胞移植对大鼠脊髓损伤区域神经损伤的修复效果。方法选择70只大鼠,雄性,体重220-260g。采用随机分组的方法将其分为研究组和对照组。大鼠均给予脊髓左侧半切,研究组在脊髓半切的基础上行脐带间充质干细胞移植。术后24h、1周、2周、4周后采用Tarlov运动功能评分评估运动功能,术后2、4周进行组织学评定。结果移植后24h,两组Tarlov评分均明显降低(均P>0.05),移植后研究组大鼠的Tarlov评分均明显优于对照组大鼠(均P<0.05)。组织学检查结果显示,对照组大鼠脊髓内灰白质界限不清、空洞面积大、可见空泡样坏死灶、神经纤维相对稀疏、混乱,且存在不同程度的炎性细胞浸润,星型胶质细胞明显聚集增生。移植后4周,研究组大鼠炎性细胞浸润,星型胶质细胞显著降低,且有神经纤维通过大鼠脊髓损伤区域(P<0.05)。结论脐带间充质干细胞移植在一定程度上可以减轻大鼠损伤脊髓部位的炎性细胞浸润,抑制胶质细胞形成瘢痕组织,对于改善大鼠神经损伤后功能的恢复有重要的意义。 展开更多
关键词 干细胞 移植 脊髓损伤 修复效果
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Dietary approach and gut microbiota modulation for chronic hepatic encephalopathy in cirrhosis 预览
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作者 Daniela Campion Ilaria Giovo +3 位作者 Paola Ponzo Giorgio M Saracco Federico Balzola Carlo Alessandria 《世界肝病学杂志:英文版(电子版)》 2019年第6期489-512,共24页
Hepatic encephalopathy(HE)is a common and serious neuropsychiatric complication of cirrhosis,acute liver failure,and porto-systemic shunting.HE largely contributes to the morbidity of patients with liver disease,sever... Hepatic encephalopathy(HE)is a common and serious neuropsychiatric complication of cirrhosis,acute liver failure,and porto-systemic shunting.HE largely contributes to the morbidity of patients with liver disease,severely affecting the quality of life of both patients and their relatives and being associated with poor prognosis.Its presentation is largely variable,manifesting with a broad spectrum of cognitive abnormalities ranging from subtle cognitive impairment to coma.The pathogenesis of HE is complex and has historically been linked with hyperammonemia.However,in the last years,it has become evident that the interplay of multiple actors,such as intestinal dysbiosis,gut hyperpermeability,and neuroinflammation,is of crucial importance in its genesis.Therefore,HE can be considered a result of a dysregulated gut-liverbrain axis function,where cognitive impairment can be reversed or prevented by the beneficial effects induced by“gut-centric”therapies,such as non-absorbable disaccharides,non-absorbable antibiotics,probiotics,prebiotics,and fecal microbiota transplantation.In this context dietary modifications,by modulating the intestinal milieu,can also provide significant benefit to cirrhotic patients with HE.This review will provide a comprehensive insight into the mechanisms responsible for gut-liver-brain axis dysregulation leading to HE in cirrhosis.Furthermore,it will explore the currently available therapies and the most promising future treatments for the management of patients with HE,with a special focus on the dietary approach. 展开更多
关键词 CIRRHOSIS Hepatic encephalopathy DIET therapy GUT MICROBIOTA Leaky GUT HYPERAMMONEMIA Prebiotics Probiotics Gluten-casein free DIET GUT MICROBIOTA transplantation
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