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基于FPGA的高可靠EDAC系统设计 预览
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作者 于善鹏 占丰 姜连祥 《质量与可靠性》 2019年第2期29-33,共5页
为减少单粒子效应对存储器造成的数据错误影响,目前卫星上多采用汉明码编码方式实现的错误检测与纠正(EDAC)系统进行数据保护。为减少单粒子翻转造成的影响,利用现场可编程门阵列(FPGA)将三模冗余(TMR)与(16, 8)准循环码2种技术相结合,... 为减少单粒子效应对存储器造成的数据错误影响,目前卫星上多采用汉明码编码方式实现的错误检测与纠正(EDAC)系统进行数据保护。为减少单粒子翻转造成的影响,利用现场可编程门阵列(FPGA)将三模冗余(TMR)与(16, 8)准循环码2种技术相结合,能够纠正单个存储器中的多位错误。通过4个FPGA实现的两级三模冗余系统,解决表决器模块单点失效的问题,对存储器中的数据进行保护,并通过仿真验证和可靠性分析证明系统的高可靠性。 展开更多
关键词 单粒子多位翻转 TMR (16 8)准循环码 高可靠性
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对外关系
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《新华月报》 2019年第6期127-128,共2页
15-16日应泰国外长敦·巴穆威奈邀请,国务委员兼外交部长王毅赴泰国举行战略磋商。16S,应泰国外长敦邀请,国务委员兼外交部长王毅赴清迈与敦举行战略磋商。
关键词 泰国 16S 部长
一株罕见Ihumii放线菌的分离和鉴定 预览
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作者 张维清 林宇岚 +3 位作者 甘龙杰 陈守涛 张文明 杨滨 《国际检验医学杂志》 CAS 2019年第4期508-510,共3页
放线菌大多存在于健康人口腔、上呼吸道、胃肠道和泌尿生殖道等与外界相通的腔道,是寄居人体的一种正常菌群^[1-2]。当机体免疫力减弱、口腔卫生不良、拔牙或外伤时,可侵入组织导致皮肤、皮下组织,肌肉,骨骼及中枢神经系统化脓性感染^[3... 放线菌大多存在于健康人口腔、上呼吸道、胃肠道和泌尿生殖道等与外界相通的腔道,是寄居人体的一种正常菌群^[1-2]。当机体免疫力减弱、口腔卫生不良、拔牙或外伤时,可侵入组织导致皮肤、皮下组织,肌肉,骨骼及中枢神经系统化脓性感染^[3]。根据感染的途径和涉及的器官不同,临床上分为面颈部、胸部、腹部和中枢神经系统感染。其主要特征为慢性化脓性肉芽肿病变,以向周围组织扩展形成瘘管并排出带有硫磺样颗粒的脓液为特征。绝大多数放线菌易引起内源性感染,大量应用免疫抑制剂是一个重要的诱发因素。2015年,法国科学家从一个50岁的HIV感染者粪便中首次分离出一株新的放线菌,并命名为Ihumii放线菌^[4]。福建医科大学附属第一医院于2016年收治一例由Ihumii放线菌引起的膝关节化脓性感染,现报道如下。 展开更多
关键词 Ihumii放线菌 MALDI-TOF-MS 16S RRNA
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肉鸡金黄色葡萄球菌的生物学特性研究 预览
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作者 崔明仙 白悦 +5 位作者 付岳林 许利军 陈立功 王学静 刘娜 刘聚祥 《今日畜牧兽医》 2019年第3期8-9,7共3页
为调查肉鸡跗关节肿胀的病因,从河北省发病肉鸡采集跗关节分泌物中分离、纯化10株细菌。培养特性、染色特性及生化特性的鉴定结果表明,10株细菌均为金黄色葡萄球菌。16S rRNA序列分析结果进一步证实3株代表菌属于金黄色葡萄球菌。药敏... 为调查肉鸡跗关节肿胀的病因,从河北省发病肉鸡采集跗关节分泌物中分离、纯化10株细菌。培养特性、染色特性及生化特性的鉴定结果表明,10株细菌均为金黄色葡萄球菌。16S rRNA序列分析结果进一步证实3株代表菌属于金黄色葡萄球菌。药敏试验结果显示10株分离菌耐药性较强,具有多重耐药性。动物试验结果表明,分离菌对小鼠具有较强的致病性。 展开更多
关键词 跗关节 金黄色葡萄球菌 16S RRNA 序列分析 耐药性 致病性
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16S rRNA基因检测在假体周围感染诊断中的应用 预览
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作者 塔拉提百克·买买提居马 王瑞 +4 位作者 焦洋 刘恒 李翔 杨姗 曹永平 《实用骨科杂志》 2019年第2期143-145,共3页
随着人工关节置换的数量增加,关节置换术后并发症也越来越受到人们的重视。无菌性松动(aseptic failure,AF)和假体周围感染(prosthetic joint infection,PJI)是患者面临关节翻修手术的两大常见原因,但二者在临床上却时常较难鉴别。由于... 随着人工关节置换的数量增加,关节置换术后并发症也越来越受到人们的重视。无菌性松动(aseptic failure,AF)和假体周围感染(prosthetic joint infection,PJI)是患者面临关节翻修手术的两大常见原因,但二者在临床上却时常较难鉴别。由于培养技术有限,很多不典型的PJI被误认为是AF。研究发现,PJI消耗的医疗资源和费用是AF的2.8倍[1]。2010年美国骨科医师协会有关PJI的指南指出[2],虽然初次髋和膝关节置换术后假体周围感染的发生率仅为1%~4%,但是一旦发生,对患者来说就是灾难性的。 展开更多
关键词 感染诊断 周围 假体 基因检测 RRNA 16S 人工关节置换 膝关节置换术后
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Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel 预览
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作者 LI Xiaohong XIAO Hui +7 位作者 ZHANG Wenjun LI Yongqian TANG Xuexi DUAN Jizhou YANG Zhibo WANG Jing GUAN Fang DING Guoqing 《海洋湖沼学报(英文)》 SCIE CAS CSCD 2019年第2期600-614,共15页
Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited a... Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited attention has been paid to the composition and function of aerobic and facultative bacteria in this process. For analysis in this study, ten samples were collected from rust layers on steel plates that had been immersed in seawater for diff erent periods (i.e., six months and eight years) at Sanya and Xiamen, China. The cultivable aerobic bacterial community structure as well as the number of sulfate-reducing bacteria (SRB) were analyzed in both cases, while the proportion of facultative SRB among the isolated aerobic bacteria in each sample was also evaluated using a novel approach. Bacterial abundance results show that the proportions are related to sea location and immersion time;abundances of culturable aerobic bacteria (CAB) and SRB from Sanya were greater in most corrosion samples than those from Xiamen, and abundances of both bacterial groups were greater in samples immersed for six months than for eight years. A total of 213 isolates were obtained from all samples in terms of CAB community composition, and a phylogenetic analysis revealed that the taxa comprised four phyla and 31 genera. Bacterial species composition is related to marine location;the results show that Firmicutes and Proteobacteria were the dominant phyla, accounting for 98.13% of the total, while Bacillus and Vibrio were the dominant genera, accounting for 53.06% of the total. An additional sixfacultative SRB strains were also screened from the isolates obtained and were found to encompass the genus Vibrio (four strains), Staphylococcus (one strain), and Photobacterium (one strain). It is noteworthy that mentions of Photobacterium species have so far been absent from the literature, both in terms of its membership of the SRB group and its relationship to corrosion. 展开更多
关键词 MARINE corrosive STEEL cultivable AEROBIC BACTERIA FACULTATIVE sulfate-reducing BACTERIA bacterial community composition 16S rRNA gene SEQUENCING
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The Effect of Silica Dust Exposure on the Serum Clara Cell Protein 16 Levels in Chinese Workers 预览
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作者 LIU Jing SONG Hai Yan +2 位作者 ZHU Bao Li PAN Li Ping QIAN Xiao Lian 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第1期47-50,共4页
Pneumoconiosis is a systemic occupational disease that commonly manifests as diffuse fibrosis of the lungs caused by long-term inhalation of silica dust in the production process. The development of pneumoconiosis is ... Pneumoconiosis is a systemic occupational disease that commonly manifests as diffuse fibrosis of the lungs caused by long-term inhalation of silica dust in the production process. The development of pneumoconiosis is a chronic and progressive process;once it occurs, it is irreversible, and gradually develops into lung injury and respiratory failure, and can cause death regardless of exposure to dust. The incidence of pneumoconiosis is the highest in China and the direct and indirect economic loss as a result of pneumoconiosis is approximately 9 billion yuan per year. 展开更多
关键词 SILICA DUST SERUM Clara Cell Protein 16 LEVELS OCCUPATIONAL disease
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犬咬伤伤口分离出咬伤巴斯德菌1例报道及文献复习 预览
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作者 申春梅 沈芳 +1 位作者 贺乐奇 刘红 《检验医学》 CAS 2019年第1期92-94,共3页
巴斯德菌属包括12个种3个亚种,主要为侵肺巴斯德菌、多杀巴斯德菌、咬伤巴斯德菌、产气巴斯德菌和犬巴斯德菌等。人类感染巴斯德菌属细菌多是由猫或狗抓咬引起的局部感染,全身感染不多见,一般见于有基础性疾病的患者^[1]。在由巴斯德菌... 巴斯德菌属包括12个种3个亚种,主要为侵肺巴斯德菌、多杀巴斯德菌、咬伤巴斯德菌、产气巴斯德菌和犬巴斯德菌等。人类感染巴斯德菌属细菌多是由猫或狗抓咬引起的局部感染,全身感染不多见,一般见于有基础性疾病的患者^[1]。在由巴斯德菌属细菌引起的感染中,侵肺巴斯德菌和多杀巴斯德菌较常见^[2-3],而咬伤巴斯德菌的分离率仅为4%~7%^[4-5],并常与其他细菌一起被分离^[6]。咬伤巴斯德菌不容易鉴定,可能是导致其分离率低的一个原因^[7-8]。目前国内关于咬伤巴斯德菌的文献不多,本研究报道1例由咬伤巴斯德菌引起的局部感染病例,以提高临床和微生物实验室对此类感染的认识。 展开更多
关键词 咬伤巴斯德菌 咬伤 基质辅助激光解析电离飞行时间质谱 16S RRNA
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犬粪肠球菌的分离鉴定及耐药性分析
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作者 王俊书 徐进强 +2 位作者 金红岩 封家旺 侯绍华 《黑龙江畜牧兽医》 CAS 北大核心 2019年第2期158-161,182共5页
为了鉴定从拉萨市病犬的粪便中分离出的一株细菌的种属及耐药性,试验采用了革兰氏染色、生化鉴定、16S rRNA PCR扩增和序列比对及药敏试验的方法对该菌株进行了研究。结果表明:该分离菌株为革兰氏阳性菌,经16S rRNA PCR鉴定为粪肠球菌,... 为了鉴定从拉萨市病犬的粪便中分离出的一株细菌的种属及耐药性,试验采用了革兰氏染色、生化鉴定、16S rRNA PCR扩增和序列比对及药敏试验的方法对该菌株进行了研究。结果表明:该分离菌株为革兰氏阳性菌,经16S rRNA PCR鉴定为粪肠球菌,仅对多黏菌素B和利奈唑胺表现高度敏感,对其他28种抗生素均表现耐药。说明从犬中分离出的粪肠球菌对多种抗生素都具有极高的耐药性。 展开更多
关键词 粪肠球菌 分离鉴定 16S RRNA 药敏试验 耐药性
Conjunctival microbiome changes associated with fungal keratitis:metagenomic analysis
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作者 Cheng Ge Chao Wei +2 位作者 Bao-Xia Yang Jun Cheng Yu-Sen Huang 《国际眼科杂志:英文版》 SCIE CAS 2019年第2期194-200,共7页
AIM:To investigate the ocular surface microbiome profile of patients with fungal keratitis(FK) through bacterial 16 S r DNA sequencing. METHODS:The swab samples were collected from 8 patients with FK(Group 1 from the ... AIM:To investigate the ocular surface microbiome profile of patients with fungal keratitis(FK) through bacterial 16 S r DNA sequencing. METHODS:The swab samples were collected from 8 patients with FK(Group 1 from the corneal ulcer, Group 2 from the conjunctival sac of the infected eyes, and Group 3 from the conjunctival sac of the fellow eyes) and 10 healthy eyes(Group 4 from the conjunctival sac). Bacterial 16 S rDNA V4-V5 region sequencing was performed to characterize the bacterial communities on the ocular surfaces of the patients with FK. RESULTS:Our metagenomic data showed that 97% of the sequence reads were categorized into 245 distinct bacterial genera, with 67.75±7.79 genera detected in Group 1, 73.80±13.44 in Group 2, 74.57±14.14 in Group 3, and 89.60±27.49 in Group 4. Compared with the healthy eyes(Group 4), both infected(Groups 1 and 2) and fellow eyes(Group 3) of the patients with FK showed reduced bacterial diversity and altered ocular surface microbiota compositions, with lower abundance of Corynebacterium and Staphylo coccus and higher abundances of Pseudomonas, Achromobacter, Caulobacter and Psychrobacter. CONCLUSION:Our report depicts the altered ocular surface bacterial community structures both in the affected and fellow eyes of patients with FK. These changes may contribute to the pathogenesis of FK or the increased risk for FK. 展开更多
关键词 CONJUNCTIVA MICROBIOTA bacteria METAGENOMICS 16S rDNA fungal KERATITIS
Intestinal toxicity of deoxynivalenol is limited by supplementation with Lactobacillus plantarum JM113 and consequentially altered gut microbiota in broiler chickens 预览
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作者 Shengru Wu Yanli Liu +5 位作者 Yongle Duan Fangyuan Wang Fangshen Guo Fang Yan Xiaojun Yang Xin Yang 《畜牧与生物技术杂志:英文版》 SCIE CAS CSCD 2019年第1期218-230,共13页
Background: Limited research has focused on the effect of Lactobacillus on the intestinal toxicity of deoxynivalenol(DON).The present study was conducted to investigate the role of Lactobacillus plantarum(L.plantarum)... Background: Limited research has focused on the effect of Lactobacillus on the intestinal toxicity of deoxynivalenol(DON).The present study was conducted to investigate the role of Lactobacillus plantarum(L.plantarum) JM113 in protecting against the intestinal toxicity caused by DON.Methods: A total of 144 one-day-old healthy Arbor Acres broilers were randomly distributed into 3 treatments,including the CON(basal diet),the DON(extra 10 mg/kg deoxynivalenol),and the DL(extra 1 × 109 CFU/kg L.plantarum JM113 based on DON group) treatments.The growth performance,organ indexes,intestinal morphology,pancreatic digestive enzymes,intestinal secreted immunoglobulin A(sIgA),jejunal transcriptome,and intestinal microbiota were evaluated.Results: Compared with the CON and DL groups,the DON supplementation altered intestinal morphology,especially in duodenum and jejunum,where villi were shorter and crypts were deeper(P < 0.05).Meanwhile,the significantly decreased mRNA expression of jejunal claudin-1 and occludin(P < 0.05),ileal rBAT and jejunal GLUT1 of 21-day-old broilers(P < 0.05),as well as duodenal PepT1 and ileal rBAT of 42-day-old broilers were identified in the DON group.Moreover,supplementation with L.plantarum JM113 could increase duodenal expression of IL-10 and IL-12 of 21-dayold broilers,ileal s IgA of 42-day-old broilers,and the bursa of Fabricius index of 21-day-old broilers.Further jejunal transcriptome proved that the genes related to the intestinal absorption and metabolism were significantly reduced in the DON group but a significant increase when supplemented with extra L.plantarum JM113.Furthermore,the bacteria related to nutrient utilization,including the Proteobacteria,Escherichia,Cc-115(P < 0.05),Lactobacillus and Prevotella(P < 0.1) were all decreased in the DON group.By contrast,supplementation with L.plantarum JM113 increased the relative abundance of beneficial bacterium,including the Bacteroidetes,Roseburia,Anaerofustis,Anaerostipe,and Ruminococcus bromi(P < 0.05).Specifically,the increased abun 展开更多
关键词 BROILER CHICKENS DEOXYNIVALENOL Gut microbiota Lactobacillus PLANTARUM JM113 mRNA SEQUENCING 16S rRNA gene SEQUENCING
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鸭疫里氏杆菌南昌分离株的16S rDNA鉴定与药敏分析
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作者 黄萃 陈茂金 +1 位作者 刘林峰 邬向东 《黑龙江畜牧兽医》 CAS 北大核心 2019年第5期86-88,181共4页
为了对从南昌某疑似鸭疫里氏杆菌病的病死雏鸭大脑、肝脏中分离得到的1株菌株进行16S rDNA鉴定,并筛选敏感药物,试验采用染色镜检、PCR扩增、测序与序列比对分析及药敏纸片扩散法等对分离株进行研究。结果表明:经染色镜检,分离菌呈革兰... 为了对从南昌某疑似鸭疫里氏杆菌病的病死雏鸭大脑、肝脏中分离得到的1株菌株进行16S rDNA鉴定,并筛选敏感药物,试验采用染色镜检、PCR扩增、测序与序列比对分析及药敏纸片扩散法等对分离株进行研究。结果表明:经染色镜检,分离菌呈革兰氏染色阴性,菌体为单个或成双排列的小杆菌,无芽孑包,将其命名为NC-RA1;PCR扩增得到大小约为1500bp的条带;分离株NC-RA1与鸭疫里氏杆菌16S rDNA基因同源性高达99%;分离株NC-RA1对氟苯尼考、替卡西林-克拉维酸、头胞噻肟敏感,对四环素中度敏感,对庆大霉素、卡那霉素等药物耐药。说明该分离株为鸭疫里氏杆菌,并且可以用氟苯尼考、替卡西林-克拉维酸、头范噬肪对其进行防治。 展开更多
关键词 鸭疫里氏杆菌 16S rDNA鉴定 染色镜检 PCR扩增 测序 药敏试验
Procyanidin B2 protects against diet-induced obesity and nonalcoholic fatty liver disease via the modulation of the gut microbiota in rabbits 预览
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作者 Ya-Wei Xing Guang-Tao Lei +2 位作者 Qing-Hua Wu Yu Jiang Man-Xiang Huang 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第8期955-966,共12页
BACKGROUND Procyanidins have beneficial effects on metabolic syndrome and antimicrobial activity,but the mechanisms underlying these effects are unclear.AIM To investigate the effects of procyanidin B2(PB2)on non-alco... BACKGROUND Procyanidins have beneficial effects on metabolic syndrome and antimicrobial activity,but the mechanisms underlying these effects are unclear.AIM To investigate the effects of procyanidin B2(PB2)on non-alcoholic fatty liver disease and to explore the possible mechanism.METHODS Thirty male New Zealand white rabbits were randomized into three groups.All of them were fed either a high-fat-cholesterol diet(HCD)or chow diet.HCD-fed rabbits were treated with vehicle or PB2 daily for 12 wk.Body weight and food intake were evaluated once a week.Serum biomarkers,such as total cholesterols,triglycerides,and aspartate transaminase,were detected.All rabbits were sacrificed and histological parameters of liver were assessed by hematoxylin and eosin-stained sections.Moreover,several lipogenic genes and gut microbiota(by 16S rRNA sequencing)were investigated to explore the possible mechanism.RESULTS The HCD group had higher body weight,liver index,serum lipid profile,insulin resistance,serum glucose,and hepatic steatosis compared to the CHOW group.PB2 treatment prevented HCD-induced increases in body weight and hypertriglyceridemia in association with triglyceride accumulation in the liver.PB2 also ameliorated low-grade inflammation,which was reflected by serum lipopolysaccharides and improved insulin resistance.In rabbit liver,PB2 prevented the upregulation of steroid response element binding protein 1c and fatty acid synthase and the downregulation of carnitine palmitoyltransferase,compared to the HCD group.Moreover,HCD led to a decrease of Bacteroidetes in gut microbiota.PB2 significantly improved the proportions of Bacteroidetes at the phylum level and Akkermansia at the genus level.CONCLUSION Our results indicate the possible mechanism of PB2 to improve HCD-induced features of metabolic syndrome and provide a new dietary supplement. 展开更多
关键词 PROCYANIDIN Rabbit Non-alcoholic fatty liver disease GUT MICROBIOTA 16S rRNA
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Bacterial Diversity in Alpine Lakes: A Review from the Third Pole Region
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作者 Namita Paudel Adhikari Subash Adhikari +2 位作者 Xiaobo Liu Liang Shen Zhengquan Gu 《地球科学学刊:英文版》 SCIE CAS CSCD 2019年第2期387-396,共10页
Microorganisms are unique among all of the living organisms because of their high population size, advanced genetic diversity, short generation time, and quick response to the small change in environmental conditions.... Microorganisms are unique among all of the living organisms because of their high population size, advanced genetic diversity, short generation time, and quick response to the small change in environmental conditions. Remote alpine lakes of the Third Pole region provide the unique habitat for microorganisms acting as a natural laboratory and offering the information about the ecological roles of microorganisms. Many researchers focused on microbial communities as well as the impact of physicochemical, biological and hydrological parameters in lakes of this region since decades but the comprehensive review focusing on bacterial diversity and the role of environmental parameters still lacks. Here we reviewed bacterial diversity in lakes of the Third Pole region by analyzing 16 S rRNA clone libraries accessed from previous research findings. A total of 5 388 bacterial 16 S rRNA gene sequences were analyzed and classified into different phylogenetic groups. The average relative abundance of dominant taxa includes Betaproteobacteria(19%), Bacteroidetes(18%), Gammaproteobacteria(16%), Actinobacteria(15%), Alphaproteobacteria(14%), Cyanobacteria(7%), and Firmicutes(5%). Several adaptational strategies were adopted by these dominant bacterial groups in order to accommodate in the respective habitat. Nevertheless, lake water properties like temperature, pH, salinity, incident UV radiation, turbidity, and nutrients also played role in bacterial diversity. 展开更多
关键词 BACTERIAL DIVERSITY ALPINE LAKES 16S rRNA THIRD Pole region
猪产气巴氏杆菌广东株的分离鉴定及其16S rDNA基因序列遗传进化分析 预览
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作者 陈胜男 翟颀 +8 位作者 李垦科 张杰 钟汝情 陈晓倩 谢莹 焦思思 冉承峰 翟少伦 邹育根 《中国动物保健》 2019年第3期53-55,共3页
产气巴氏杆菌是一种人兽共患性病原菌,可引起人和动物不同程度的流产、不孕等繁殖障碍性疾病。本文对广东某猪场采集的母猪产道分泌物进行病原分离检测,在产道拭子中分离到一种疑似巴氏杆菌的菌体。经细菌16S rDNA 基因序列扩增、测序,... 产气巴氏杆菌是一种人兽共患性病原菌,可引起人和动物不同程度的流产、不孕等繁殖障碍性疾病。本文对广东某猪场采集的母猪产道分泌物进行病原分离检测,在产道拭子中分离到一种疑似巴氏杆菌的菌体。经细菌16S rDNA 基因序列扩增、测序,鉴定为猪产气巴氏杆菌,命名为GD01 株。序列分析显示GD01 株与GenBank 数据库中公布的产气巴氏杆菌16SrDNA 核酸同源性为98.5%以上。本研究首次报道了猪产气巴氏杆菌在广东的流行,其在猪群的流行病学意义有待进一步调查研究。 展开更多
关键词 产气巴氏杆菌 繁殖障碍 16S rDNA 遗传进化
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虹鳟荧光假单胞菌的分离鉴定 预览
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作者 何文涛 谢洪霞 《甘肃畜牧兽医》 2019年第2期57-59,共3页
为鉴定分析出甘肃省永登县某养鳟场疑似爆发赤皮病的病因,通过对发病虹鳟鱼进行临床症状、病理变化的观察,对发病鱼主要器官进行细菌的分离培养,并对分离培养的细菌利用16S rRNA序列分析,对菌株进行鉴定及遗传进化分析。结果表明,成功... 为鉴定分析出甘肃省永登县某养鳟场疑似爆发赤皮病的病因,通过对发病虹鳟鱼进行临床症状、病理变化的观察,对发病鱼主要器官进行细菌的分离培养,并对分离培养的细菌利用16S rRNA序列分析,对菌株进行鉴定及遗传进化分析。结果表明,成功分离出一株荧光假单胞菌,将其16S rRNA基因序列与GenBank中登录的荧光假单胞菌参考株的16S rRNA基因序列进行比对,同源率达到99%。经MEGA7.0软件分析后,其位于不同的分支上。本研究结果为鳟鱼赤皮病的快速检测和对甘肃永登地区赤皮病的有效防治提供理论依据。 展开更多
关键词 赤皮病 荧光假单胞菌 分离鉴定 16S RRNA基因
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3株松鼠葡萄球菌的鉴定及耐药性分析
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作者 王果帅 王鹏勇 +1 位作者 陈革 王彦红 《黑龙江畜牧兽医》 CAS 北大核心 2019年第4期96-97,101共3页
为了对从2017年送检的3个病例中分离得到的病料进行病原菌鉴定,试验采用细菌分离、纯化、生化鉴定及16S rDNA基因PCR扩增与序列分析法进行鉴定,并对分离菌进行药敏试验。结果表明:共分离得到3株分离菌,分别命名为J1、J2、J3株,3株分离... 为了对从2017年送检的3个病例中分离得到的病料进行病原菌鉴定,试验采用细菌分离、纯化、生化鉴定及16S rDNA基因PCR扩增与序列分析法进行鉴定,并对分离菌进行药敏试验。结果表明:共分离得到3株分离菌,分别命名为J1、J2、J3株,3株分离菌均能发酵蔗糖和麦芽糖,产酸,不能发酵甘露醇和乳糖;PCR扩增分离菌,得到500 bp的目的条带,与NCBI中已知序列进行比对,均为松鼠葡萄球菌(同源性达99%);3株松鼠葡萄球菌对多西环素、阿莫西林、环丙沙星等药物敏感。说明3株分离菌为松鼠葡萄球菌,其对药物有不同程度的敏感性。 展开更多
关键词 松鼠葡萄球菌 分离 鉴定 耐药性 16S RDNA
一株致水貂流产的大肠杆菌的分离鉴定及耐药性检测
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作者 肖丽荣 周琦 +5 位作者 李巧玲 贾青辉 张艳英 高桂生 张召兴 史秋梅 《黑龙江畜牧兽医》 CAS 北大核心 2019年第6期156-158,共3页
为检测唐山某水貂养殖场引起母貂流产的病原菌及其耐药情况,试验采用病原检测、细菌分离与形态观察、生化试验、16SrRNAPCR检测、致病性试验等方法对水貂致病原进行鉴定,并采用药敏试验测定其耐药情况。结果表明:水貂阿留申病毒、犬瘟... 为检测唐山某水貂养殖场引起母貂流产的病原菌及其耐药情况,试验采用病原检测、细菌分离与形态观察、生化试验、16SrRNAPCR检测、致病性试验等方法对水貂致病原进行鉴定,并采用药敏试验测定其耐药情况。结果表明:水貂阿留申病毒、犬瘟热病毒、细小病毒、加德纳杆菌和布鲁氏杆菌等病原检测为阴性;从无菌采集的阴道分泌物及胎儿等病料组织中分离到1株病原菌,将其命名为HB-E2株,该株病原菌为两端钝圆、短小杆状的革兰氏阴性菌,通过PCR检测鉴定为大肠杆菌;HB-E2株大肠杆菌对小白鼠具有很强的致病性,对头胞曲松、头胞克肪、环丙沙星等5种药物高度敏感,对氟苯尼考、头胞拉定、阿米卡星等5种药物中度敏感,对氨节西林、阿莫西林、新霉素等6种药物耐药。 展开更多
关键词 水貂 大肠杆菌 分离鉴定 16S RRNA PCR 药敏试验
Relationship between intestinal microbial dysbiosis and primary liver cancer 预览
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作者 Lei Zhang Yong-Na Wu +3 位作者 Tuo Chen Cheng-Hui Ren Xun Li Guang-Xiu Liu 《国际肝胆胰疾病杂志:英文版》 SCIE CAS CSCD 2019年第2期149-157,共9页
Background:Intestinal microbial dysbiosis is involved in liver disease pathogenesis.However,its role in primary liver cancer(PLC),particularly in hepatocarcinogenesis remains unclear.The present study aimed to study t... Background:Intestinal microbial dysbiosis is involved in liver disease pathogenesis.However,its role in primary liver cancer(PLC),particularly in hepatocarcinogenesis remains unclear.The present study aimed to study the changes in intestinal flora at various stages of PLC and clarify the relationship between intestinal microbes and PLC.Methods:Twenty-four patients with PLC(PLC group),24 patients with liver cirrhosis(LC group),and 23 healthy control individuals(HC group)were enrolled from October 2016 to October 2017.Stool specimens of the participants were collected and the genomic DNA of fecal bacteria was isolated.High-throughput pyrosequencing of 16S rDNA was used to identify differences in gut bacterial diversity among HC,LC,and PLC groups.We also analyzed the relationship between clinical factors and intestinal microorganisms in LC and PLC groups.Results:Diversity of Firmicutes tended to decrease from the HC to LC and PLC groups at the phylum level.Among species,Enterobacter ludwigii displayed an increasing trend in the PLC group,wherein the relative abundance of Enterobacter ludwigii in the PLC group was 100 times greater than that in the HC and LC groups.The ratio of Firmicutes/Bacteroidetes was significantly decreased with the disease progression.In addition,the linear discriminant analysis effect size method indicated that Clostridia were predominant in the gut microbiota of the HC group,whereas Enterococcaceae,Lactobacillales,Bacilli and Gammaproteobacteria may be used as diagnostic markers of PLC.Redundancy analysis showed a correlation between intestinal microbial diversity and clinical factors AST,ALT,and AFP.Veillonella showed a significant positive correlation with AFP in the PLC group,whereas Subdoligranulum showed a negative correlation with AFP.Conclusions:This study indicates that dysbiosis of the gut microbiota might be involved in PLC development and progression. 展开更多
关键词 GUT MICROBIOTA MICROBIAL DYSBIOSIS HEPATOCARCINOGENESIS 16S rDNA
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新型16SrRNA基因芯片在新生儿败血症病原检测中的价值研究
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作者 胡晓艳 徐颂周 +3 位作者 吴宇亮 李科铮 王存艳 周于新 《中华新生儿科杂志》 CAS 2018年第5期325-328,共4页
目的探讨新型16S rRNA基因芯片在新生儿败血症病原检测中的价值。方法选择2015年1月至2017年12月北京大学深圳医院新生儿科收治的疑似败血症新生儿为研究对象,对所有新生儿抽取静脉血分别用血培养和基因芯片法进行病原检测,比较两种... 目的探讨新型16S rRNA基因芯片在新生儿败血症病原检测中的价值。方法选择2015年1月至2017年12月北京大学深圳医院新生儿科收治的疑似败血症新生儿为研究对象,对所有新生儿抽取静脉血分别用血培养和基因芯片法进行病原检测,比较两种方法检测的阳性率、检测所需时间和检测所需血量。结果共纳入306例疑似败血症新生儿,其中血培养阳性34例(11.1%),基因芯片法阳性54例(17.6%);98例诊断为新生儿败血症,其中血培养阳性34例(34.7%),基因芯片法阳性52例(53.1%),基因芯片法阳性率均高于血培养(P〈0.05);基因芯片法对新生儿败血症5种常见病原的检出率高于血培养。血培养报阳时间为(14.6±5.5)h,鉴定病原时间为(72.9±19.0) h,基因芯片法报阳时间和鉴定病原时间均为3 h,基因芯片法检测病原所需时间明显短于血培养(P〈0.001)。血培养耗血量1~2 ml,基因芯片法耗血量0.5 ml,基因芯片法耗血量少于血培养。结论与传统血培养相比,基因芯片法能快速检测血中病原菌,有较高的阳性率,并可减少采血量,通过不断改进与完善,在新生儿败血症诊断中有较大的应用价值。 展开更多
关键词 RNA 核糖体 16S 病原 基因芯片 败血症 婴儿 新生
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