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呼吸科病房老年病人肺部感染常见病原菌及其耐药性分析 预览
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作者 褚月娇 周楚铭 +3 位作者 韩莹莹 刘丹 万小旭 王佳贺 《安徽医药》 CAS 2019年第1期63-66,共4页
目的对老年病人(年龄≥65岁)肺部感染的常见病原菌及其耐药性进行分析,为临床预防和治疗老年肺部感染提供依据。方法回顾性收集中国医科大学附属盛京医院2013年1月至2015年12月老年病人感染的常见病原菌并对其耐药性进行分析。结果共分... 目的对老年病人(年龄≥65岁)肺部感染的常见病原菌及其耐药性进行分析,为临床预防和治疗老年肺部感染提供依据。方法回顾性收集中国医科大学附属盛京医院2013年1月至2015年12月老年病人感染的常见病原菌并对其耐药性进行分析。结果共分离出318株病原菌,其中革兰阴性杆菌295例,占92.77%。其中鲍曼不动杆菌最多,占43.71%;其次是铜绿假单胞菌和肺炎克雷伯杆菌,分别占16.98%和12.89%。共分离出革兰阳性球菌20例,占6.29%。真菌3例,占0.94%。不同病原菌耐药程度不同,其中鲍曼不动杆菌对青霉素类、头孢类抗生素耐药率达90%~100%,对氨基糖苷类抗生素耐药率亦达38%~90%,仅替加环素耐药率为10.31%。铜绿假单胞菌耐药亦较严重。肺炎克雷伯杆菌耐药率相对乐观,临床可选药物较多。金黄色葡萄球菌对青霉素、头孢类抗生素几乎均耐药,对万古霉素、利奈唑胺、喹努普汀/达福普汀及替加环素最为敏感。结论老年肺部感染以革兰阴性杆菌为主,临床应根据药敏实验结果合理应用抗生素来更好地控制疾病和防止产生耐药菌。 展开更多
关键词 呼吸道感染 抗药性 细菌 抗药性 真菌 革兰阳性菌感染 革兰阴性菌感染 病房 老年人
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Bacterial community succession in response to dissolved organic matter released from live jellyfish 预览
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作者 HAO Wenjin WICHELS Antje +2 位作者 FUCHS Bernhardt TANG Xuexi GERDTS Gunnar 《海洋湖沼学报(英文)》 SCIE CAS CSCD 2019年第4期1229-1244,共16页
Jellyfish blooms have increased worldwide, and the outbreaks of jellyfish population not only affect the food web structures via voracious predation but also play an important role in the dynamics of nutrients and oxy... Jellyfish blooms have increased worldwide, and the outbreaks of jellyfish population not only affect the food web structures via voracious predation but also play an important role in the dynamics of nutrients and oxygen in planktonic food webs. However, it remains unclear whether specific carbon compounds released through jellyfish metabolic processes have the potential to shape bacterial community composition. Therefore, in this study, we aimed to investigate the compositional succession of the bacterioplankton community in response to the dissolved organic matter (DOM) released by the live Scyphomedusae Cyanea lamarckii and Chrysaora hysoscella collected from Helgoland Roads of the North Sea. The bacterial community was significantly stimulated by the DOM released form live jellyfish and different dominant phylotypes were observed for these two Scyphomedusae species. Furthermore, the bacterial community structures in the different DOM sources, jellyfish-incubated media, Kabeltonne seawater, and artificial seawater (DOM-free) were significantly different, as revealed by automated ribosomal intergenic spacer analysis fingerprints. Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) revealed a rapid species-specific shift in bacterial community composition. Gammaproteobacteria dominated the community instead of the Bacteroidetes community for C. lamarckii, whereas Gammaproteobacteria and Bacteroidetes dominated the community for C. hysoscella. The significant differences in the bacterial community composition and succession indicate that the components of the DOM released by jellyfish might differ with jellyfish species. 展开更多
关键词 BACTERIAL COMMUNITY structure BACTERIAL COMMUNITY composition dissolved organic matter JELLYFISH automated rRNA INTERGENIC spacer analysis (ARISA) catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH)
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抗菌药物专项管理对使用指标及革兰阴性多重耐药菌检出率的影响
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作者 徐玉兰 胡丽敏 +2 位作者 谢作楷 董翌玮 董琳 《中华儿科杂志》 CAS CSCD 北大核心 2019年第7期553-558,共6页
目的分析实施抗菌药物专项管理前后使用指标及革兰阴性多重耐药菌(MDR-GNB)检出率的变化,评估实施专项管理的效果。方法温州医科大学附属第二医院、育英儿童医院于2011年12月开始开展抗菌药物专项管理活动,2014—2017年进一步加大管理... 目的分析实施抗菌药物专项管理前后使用指标及革兰阴性多重耐药菌(MDR-GNB)检出率的变化,评估实施专项管理的效果。方法温州医科大学附属第二医院、育英儿童医院于2011年12月开始开展抗菌药物专项管理活动,2014—2017年进一步加大管理力度。回顾性分析管理前(2010—2011年)、管理后(2012—2013年为第一阶段、2014—2015年为第二阶段、2016—2017年为第三阶段),各阶段全院抗菌药物门诊、急诊、住院患者使用率及抗菌药物使用强度(AUD)、第三代头孢菌素AUD和碳青霉烯类抗菌药物AUD;统计同期产超广谱β内酰胺酶(ESBLs)大肠埃希菌和肺炎克雷伯菌,耐碳青霉烯类抗菌药物大肠埃希菌和肺炎克雷伯菌,耐碳青霉烯类抗菌药物鲍曼不动杆菌和铜绿假单胞菌的检出率,并与各抗菌药物使用指标作相关性分析。结果管理前和管理后三个阶段门诊抗菌药物使用率分别为55.2%(560 578/1 015 540)、38.1%(493 554/1 296 336)、26.8%(378 602/1 411 595)、23.1%(347 817/1 502 817);急诊抗菌药物使用率为75.6%(429 582/568 230)、61.4%(382 558/623 138)、43.6%(265 102/608 071)、35.1%(218 484/622 397),住院抗菌药物使用率为76.0%(30 568/40 221)、53.7%(30 437/56 636)、49.9%(37 395/74 895)、50.3%(35 493/70 544),整治后均明显下降(χ^2=297 811.798、3 155 704.783、5 592.037,P均<0.01);住院患者AUD分别为38.43、31.80、21.65、19.41,第三代头孢菌素AUD分别为13.83、11.21、6.20、6.84,管理后均明显下降(r=-0.878,-0.781,P均<0.05),碳青霉烯类抗菌药物AUD分别为1.94、1.77、1.87、1.93,无显著变化(r=0.123,P>0.05)。共收集细菌11 289株,其中大埃希菌5 589株,肺炎克雷伯菌2 823株,鲍曼不动杆菌1 637株,铜绿假单胞菌1 240株,管理前、管理后三个阶段产ESBLs大肠埃希菌检出率分别为75.4%(1 034/1 371)、66.6%(893/1 341)、57.8%(834/1 443)、46.7%(670/1 434);产ESBLs肺炎克雷伯菌检出率为78.7%(547/695)、67.5%(455/674)、49.3%( 展开更多
关键词 革兰氏阴性菌 抗药性 细菌 抗菌药 儿童
Bacterial communities fluctuate in abundance and diversity under simulated oil-contaminated seawater conditions 预览
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作者 LI Xiaohong YOU Cai +3 位作者 QU Liang ZHOU Bin TANG Xuexi XIAO Hui 《海洋湖沼学报(英文)》 SCIE CAS CSCD 2019年第2期615-627,共13页
Marine bacteria have recently been identified as a potent solution for petroleum hydrocarbon degradation in response to hazardous oceanic oil spills. In this study, a mesocosm experiment simulating a petroleum spill e... Marine bacteria have recently been identified as a potent solution for petroleum hydrocarbon degradation in response to hazardous oceanic oil spills. In this study, a mesocosm experiment simulating a petroleum spill event was performed to investigate changes in the abundance, structure, and productivity of bacterial communities in response to oil pollution. Cultured heterotrophic bacteria and total bacteria showed a consistent trend involving an immediate decrease in abundance, followed by a slight increase, and a steady low-level thereafter. However, the changing trend of bacterial productivity based on bacterial biomass and bacterial volume showed the opposite trend. In addition, the density of oil-degrading bacteria increased initially, then subsequently declined. The change in the bacterial community structure at day 0 and day 28 were also analyzed by amplified ribosomal DNA restriction analysis (ARDRA), which indicated that the species diversity of the bacterial community changed greatly after oil pollution. Alphaproteobacteria (40.98%) replaced Epsilonproteobacteria (51.10%) as the most abundant class, and Gammaproteobacteria (38.80%) became the second most dominant class in the whole bacterial community. The bacterial communities in oil-contaminated seawater (32 genera) became much more complex than those found in the natural seawater sample (16 genera). The proportion of petroleum-degrading bacteria in the oil-contaminated seawater also increased. In this study, culture-dependent and culture-independent approaches were combined to elucidate changes in both bacterial productivity and community structure. These findings will contribute to a better understanding of the role that bacteria play in material cycling and degradation in response to oil pollution. 展开更多
关键词 petroleum pollution BACTERIAL community BACTERIAL growth amplified RIBOSOMAL DNA RESTRICTION analysis (ARDRA)
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Microbial aerosol chemistry characteristics in highly polluted air
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作者 Ting Zhang Xinyue Li +2 位作者 Minfei Wang Haoxuan Chen Maosheng Yao 《中国科学:化学英文版》 SCIE EI CAS CSCD 2019年第8期1051-1063,共13页
Aerosol chemistry is often studied without considering microbial involvements. Here, we investigated time-and size-resolved bacterial aerosol dynamics in air. Under high particulate matter(PM) polluted episodes, bacte... Aerosol chemistry is often studied without considering microbial involvements. Here, we investigated time-and size-resolved bacterial aerosol dynamics in air. Under high particulate matter(PM) polluted episodes, bacterial aerosols exhibited a viability of up to 50%–70% in the 0.56–1 μm size range, at which elevated levels of SO42-, NO3- and NH4+ were concurrently observed.Engineered or acclimated for both industrial use, bacteria such as Psychrobacter spp., Massilia spp., Acinetobacter lwoffii,Exiguobacteriumaurantiacum and Bacillusmegaterium were shown to have experienced massive abundance shifts in polluted air on early mornings and late afternoons, on which rapid new particle formation events were widely reported. Here, Psychrobacter spp. were shown to account for >96% abundance at a corresponding PM2.5 level of 208 μg/m3. These observed bacterial aerosol changes corresponded to the PM2.5 mass peak shift from 3.2–5.6 μm to the high viability size range of 0.56–1 μm. Additionally,elevated levels of soluble Na, Ca, Mg, K, Al, Fe and P elements, required for bacterial growth, were observed to co-occur with those significant bacterial aerosol structure shifts in the air. For particular time-resolved PM2.5 pollution episodes, Acinetobacter,Psychrobacter and Massilia were shown to alternate in dominating the time-resolved aerosol community structures. The results from a HYSPLIT trajectory model simulation suggested that air mass transport played a minor role in affecting the observed bacterial aerosol structure dynamics. All the data here suggested that airborne bacteria in the size range of 0.56–1 μm could be extensively involved in aerosol chemistry in highly polluted humid air. 展开更多
关键词 BACTERIAL AEROSOL dynamics in AIR PARTICULATE matter size-and TIME-RESOLVED BACTERIAL AEROSOL AEROSOL chemistry
浙江省宁波市第六医院铜绿假单胞菌的临床分布及耐药性分析
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作者 孙永宁 王福斌 《中国基层医药》 CAS 2019年第1期86-90,共5页
目的探讨宁波市第六医院铜绿假单胞菌的耐药性情况,为临床合理选用抗菌药物提供依据。方法回顾性调查分析宁波市第六医院2014-2016年1970株铜绿假单胞菌临床分布及对常用抗菌药物的耐药性;数据采用WHONET5.6软件、EXCEL2010软件、SPSS1... 目的探讨宁波市第六医院铜绿假单胞菌的耐药性情况,为临床合理选用抗菌药物提供依据。方法回顾性调查分析宁波市第六医院2014-2016年1970株铜绿假单胞菌临床分布及对常用抗菌药物的耐药性;数据采用WHONET5.6软件、EXCEL2010软件、SPSS17.0软件进行统计分析。结果临床标本分离15963株病原菌,其中铜绿假单胞菌检出1970株,占12.34%,多重耐药铜绿假单胞菌(MDRPA)检出率逐年降低;其中2014年检出率为60.95%,2015年为58.00%,2016年为45.58%。铜绿假单胞菌主要分离自痰液占67.16%,其次为伤口分泌物占23.05%;ICU及老年医学科铜绿假单胞菌检出率较高,分别占20.25%及25.28%;2014-2016年铜绿假单胞菌对多种抗菌药物的耐药性均呈上升趋势,头孢哌酮/舒巴坦3年中耐药率均>30%,亚胺培南的耐药性高于美罗培南。ICU与老年医学科痰液中分离的铜绿假单胞菌药物耐药率相比较,ICU检出的铜绿假单胞菌的耐药率高于老年医学科(均P<0.05)。结论宁波市第六医院铜绿假单胞菌医院感染情况严重,应加强对其感染率和耐药性的监测,以降低感染率和耐药性。 展开更多
关键词 假单胞菌 铜绿 抗药性 细菌 感染 抗菌药 小地区分析
Effect of nitrobenzene on the performance and bacterial community in an expanded granular sludge bed reactor treating high-sulfate organic wastewater
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作者 Jun Li Wentao Li +2 位作者 Gan Luo Yan Li Aimin Li 《中国环境科学与工程前沿:英文版》 SCIE EI CAS CSCD 2019年第1期63-72,共10页
Nitrobenzene (NB) is frequently found in wastewaters containing sulfate and may affect biological sulfate reduction process, but information is limited on the responses of sulfate reduction efficiency and microbial co... Nitrobenzene (NB) is frequently found in wastewaters containing sulfate and may affect biological sulfate reduction process, but information is limited on the responses of sulfate reduction efficiency and microbial community to the increased NB contents. In this study, a laboratory-scale expanded granular sludge bed reactor was operated continuously to treat high-sulfate organic wastewater with increased NB contents. Results successfully demonstrated that the presence of more than 50 mg/L NB depressed sulfate reduction and such inhibition was partly reversible. Bath experiments showed that the maximum specific desulfuration activity (SDA) decreased from 135.80 mg SO4^2-/gVSS/d to 30.78 mg SO4^2-/gVSS/d when the NB contents increased from none to 400 mg/L. High-throughput sequencing showed that NB also greatly affected bacterial community structure. Bcicteroidetes dominated in the bioreactor. The abundance of Proteobacteria increased with NB addition while Firmicutes presented an opposite trend. Proteobacteria gradually replaced Finnicutes for the dominance in response to the increase of influent NB concentrations.The genus Desulfovibrio was the dominant sulfate-reducing bacteria (SRB) with absence or presence of NB, but was inhibited under high content of NB. The results provided better understanding for the biological sulfate reduction under NB stress. 展开更多
关键词 NITROBENZENE (NB) Sulfate-reducing bacteria (SRB) BACTERIAL community SULFATE reduction HIGH-THROUGHPUT SEQUENCING
合并急性肾损伤的感染性心内膜炎的临床特点和预后分析 预览
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作者 张思宇 李西慧 肖锋 《北京大学学报:医学版》 CAS CSCD 北大核心 2019年第4期737-741,共5页
目的:探讨合并急性肾损伤(acute kidney injury,AKI)的感染性心内膜炎(infective endocarditis,IE)患者的临床特点,分析此类患者的围术期及预后情况。方法:回顾性分析2002年1月至2018年6月在北京大学第一医院心脏外科就诊的IE患者100例... 目的:探讨合并急性肾损伤(acute kidney injury,AKI)的感染性心内膜炎(infective endocarditis,IE)患者的临床特点,分析此类患者的围术期及预后情况。方法:回顾性分析2002年1月至2018年6月在北京大学第一医院心脏外科就诊的IE患者100例。根据患者术前情况及AKIN标准,分为AKI组和非AKI组,比较两组临床资料和术后并发症的差异,随访对比两组预后情况。结果: AKI组21例和非AKI组79例,总体平均年龄(43.7±15.7)岁,男女比例3 ∶ 1,IE患者中AKI的发生率为21%。两组间年龄、性别构成差异无统计学意义。与非AKI组相比,AKI组患者皮疹及下肢水肿的比例更高( P =0.017和 P =0.001),尿潜血及尿蛋白阳性率更高( P <0.001),血红蛋白及血清白蛋白水平更低( P <0.001),临床心功能更差( P =0.033)。两组病原菌检出率及病原菌种类差异无统计学意义。除9例患者拒绝手术外,其余91例患者均接受了体外循环下心内直视手术,包括AKI组19例和非AKI组72例。AKI组患者围术期红细胞用量更多( P =0.010),术后呼吸机使用时间及监护室停留时间更长( P =0.028和 P =0.003)。以两组患者术前末次肌酐为基准,AKI组新发术后肾功能不全比例更高( P =0.004)。两组患者院内死亡率差异无统计学意义( P =0.463),随访期间两组生存率差异未见统计学意义( P =0.581)。结论:与非AKI组相比,合并AKI的IE患者围术期并发症发生率更高,但两组患者院内死亡率及预后无明显差异。 展开更多
关键词 急性肾损伤 心内膜炎 细菌性 心脏外科手术
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低蛋白血症对神经重症患者肺部感染的预后影响分析 预览
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作者 范玉兰 李凤 谭家泽 《现代医药卫生》 2019年第5期644-646,649共4页
目的了解低蛋白血症对神经重症患者肺部感染的预后影响。方法将2010年6月至2016年6月该院神经内科重症监护病房(NICU)收治的有肺部感染且痰培养阳性的已出院患者316例作为研究对象。将肺部感染合并低蛋白血症患者198例作为试验组,且根... 目的了解低蛋白血症对神经重症患者肺部感染的预后影响。方法将2010年6月至2016年6月该院神经内科重症监护病房(NICU)收治的有肺部感染且痰培养阳性的已出院患者316例作为研究对象。将肺部感染合并低蛋白血症患者198例作为试验组,且根据血清清蛋白水平分为轻、中、重度,分别为50、67、81例。肺部感染未合并低蛋白血症患者118例作为对照组,对两组患者的预后进行分析。结果试验组急性生理学及慢性健康状况评分(APACHEⅡ)、平均住院时间、NICU住院时间、患者疾病转归与对照组比较,差异均有统计学意义(P=0.014、0.010、0.047、0.013)。结论神经重症患者肺部感染合并低蛋白血症预后差,有效防治低蛋白血症有可能预防和减轻肺部感染,对患者的成功救治及预后有积极意义。 展开更多
关键词 低蛋白血症 神经系统疾病 肺炎 细菌性 疾病转归 预后
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2018年某三级医院细菌耐药性监测结果分析 预览
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作者 张世武 王龙 +1 位作者 杨淮丽 王自林 《中国临床保健杂志》 CAS 2019年第4期472-476,共5页
目的 了解某三级医院2018年临床分离的病原菌分布特点及其对常用抗菌药物的耐药情况,为临床用药提供依据和参考。方法 总共收集门诊和住院患者各类培养标本13499份,经培养分离出1623株非重复有效菌株,采用自动仪器法或纸片扩散法(K-B)... 目的 了解某三级医院2018年临床分离的病原菌分布特点及其对常用抗菌药物的耐药情况,为临床用药提供依据和参考。方法 总共收集门诊和住院患者各类培养标本13499份,经培养分离出1623株非重复有效菌株,采用自动仪器法或纸片扩散法(K-B)对其非重复的1623株进行体外药敏实验,按CLSI2016年版判读药敏结果,采用WHONET5.6软件进行耐药性分析。结果 本院共分离病原菌1623株,其中革兰阴性菌占66.4%(1078),革兰阳性菌占33.6%(545)。10种最常见的细菌分别为:大肠埃希菌(329株),肺炎克雷伯肺炎亚种(252株),铜绿假单胞菌(114株),表皮葡萄球菌(102株),金黄色葡萄球菌(101株),鲍曼氏不动杆菌(100株),人葡萄球菌(80株),溶血性葡萄球菌(69株),粪肠球菌(44株),阴沟肠杆菌(33株)。葡萄球菌中MRSA和MRCNS的检出率分别为11.8%和60.1%,未发现万古霉素和替考拉宁耐药菌株。屎肠球菌对常用抗生素的耐药率明显高于粪肠球菌,其中屎肠球菌和粪肠球菌对万古霉素耐药率分别为7.1%和2.4%,依据表型推测可能为VanA和VanB型耐药。肠杆菌科细菌中,肺炎克雷伯菌对抗菌药物亚胺培南和美罗培南的耐药率分别为22.8%和22.1%,明显高于大肠埃希菌和阴沟肠杆菌等其他肠杆菌科细菌。非发酵菌主要以铜绿假单胞菌和鲍曼氏不动杆菌为主,其中铜绿假单胞菌对氨苄西林、头孢唑啉、哌拉西林/他唑巴坦、氨苄西林/舒巴坦、头孢噻肟、复方新诺明等耐药率达到100%,对亚胺培南和左氧氟沙星的耐药率高达30%以上,鲍曼不动杆菌对左氧氟沙星、环丙沙星、亚胺培南和美罗培南的耐药率高达80%以上,与安徽省2015年细菌耐药监测分析基本一致[1]。结论 临床分离的细菌对抗生素耐药呈逐年增长趋势,尤其多重耐药和泛耐药菌的频繁检出应引起高度重视,更应加强抗菌药物的合理使用和耐药性的监测显得尤为重要。 展开更多
关键词 抗药性 细菌 药物监测 流行病学研究
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Viral and Bacterial Etiology of Acute Febrile Respiratory Syndrome among Patients in Qinghai, China 预览
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作者 LIU Gao Shan LI Hong +3 位作者 ZHAO Sheng Cang LU Rou Jian NIU Pei Hua TAN Wen Jie 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第6期438-445,共8页
Objective This study was conducted to investigate the viral and bacterial etiology and epidemiology of patients with acute febrile respiratory syndrome(AFRS) in Qinghai using a commercial routine multiplex-ligation-nu... Objective This study was conducted to investigate the viral and bacterial etiology and epidemiology of patients with acute febrile respiratory syndrome(AFRS) in Qinghai using a commercial routine multiplex-ligation-nucleic acid amplification test(NAT)-based assay. Methods A total of 445 nasopharyngeal swabs specimens from patients with AFRS were analyzed using the RespiFinderSmart22 kit(PathoFinder BV, Netherlands) and the LightCycler 480 real-time PCR system. Results Among the 225(225/445, 51%) positive specimens, 329 positive pathogens were detected, including 298(90.58%) viruses and 31(9%) bacteria. The most commonly detected pathogens were influenza virus(IFV;37.39%;123/329), adenovirus(AdV;17.02%;56/329), human coronaviruses(HCoVs;10.94%;36/329), rhinovirus/enterovirus(RV/EV;10.03%;33/329), parainfluenza viruses(PIVs;8.51%;28/329), and Mycoplasma pneumoniae(M. pneu;8.51%;28/329), respectively. Among the co-infected cases(17.53%;78/445), IFV/AdV and IFV/M. pneu were the most common co-infections. Most of the respiratory viruses were detected in summer and fall. Conclusion In our study, IFV-A was the most common respiratory pathogen among 22 detected pathogens, followed by AdV, HCoV, RV/EV, PIV, and M. pneu. Bacteria appeared less frequently than viruses, and co-infection was the most common phenomenon among viral pathogens. Pathogens were distributed among different age groups and respiratory viruses were generally active in July, September, and November. Enhanced surveillance and early detection can be useful in the diagnosis, treatment, and prevention of AFRS, as well as for guiding the development of appropriate public health strategies. 展开更多
关键词 Acute FEBRILE RESPIRATORY SYNDROME VIRAL and BACTERIAL ETIOLOGY Prevalence Seasonal distribution
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新生儿耐碳青霉烯类肺炎克雷伯菌败血症临床分析
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作者 姚恩凤 孙玄 +2 位作者 姜丽 陈中举 陈玲 《中华新生儿科杂志(中英文)》 CAS 2019年第2期119-124,共6页
目的探讨新生儿耐碳青霉烯类肺炎克雷伯菌(carbapenem-resistant klebsiella pneumonia,CRKP)败血症患儿的临床特点,以指导临床治疗。方法回顾性分析2012年1月至2017年12月华中科技大学同济医学院附属同济医院新生儿重症监护病房收治的... 目的探讨新生儿耐碳青霉烯类肺炎克雷伯菌(carbapenem-resistant klebsiella pneumonia,CRKP)败血症患儿的临床特点,以指导临床治疗。方法回顾性分析2012年1月至2017年12月华中科技大学同济医学院附属同济医院新生儿重症监护病房收治的肺炎克雷伯菌败血症患儿的临床资料。根据药物敏感试验结果分为CRKP组和碳青霉烯类敏感肺炎克雷伯菌(carbapenem-sensitive klebsiella pneumonia,CSKP)组,比较两组患儿临床表现、实验室检查、危险因素、治疗与转归等临床特点。结果研究期间共培养出肺炎克雷伯菌71株,占全部分离病原菌的33.2%(71/214)。确诊肺炎克雷伯菌败血症患儿71例,其中CRKP组35例,CSKP组36例。CRKP组呼吸困难/呼吸暂停比例高于CSKP组[85.7%(30/35)比55.6%(20/36)],差异有统计学意义(P=0.005)。单因素分析显示,CRKP组败血症患儿胎龄小于CSKP组,男性、感染前应用持续气道正压通气、感染前应用肠外营养及感染前应用抗生素(头孢菌素类、青霉素类、碳青霉烯类)比例均高于CSKP组,感染前住院时间长于CSKP组,差异有统计学意义(P<0.05)。多因素Logistic回归分析显示,男性(OR=8.125,95%CI 2.275~29.021),感染前应用肠外营养(OR=27.730,95%CI 2.948~260.858)及感染前应用碳青霉烯类抗生素(OR=4.849,95%CI 1.091~21.554)是新生儿发生CRKP败血症的独立危险因素(P<0.05)。CRKP组患儿病死率高于CSKP组[22.9%(8/35)比5.6%(2/36)],差异有统计学意义(P=0.036)。结论男性、感染前应用肠外营养及感染前应用碳青霉烯类抗生素是新生儿发生CRKP败血症的独立危险因素。CRKP败血症病情危重,病死率高,应加强耐药监测,合理管理抗菌药物,尽早建立胃肠道营养。 展开更多
关键词 克雷伯菌 肺炎 抗药性 细菌 碳青霉烯类抗生素 败血症 婴儿 新生
Bacterial Community Structure of Sediment in Upstream Ecological Wetland of Guanting Reservoir under the Action of Different Plants in Winter 预览
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作者 Yan Donghua Jiang Tiantian +4 位作者 Li Hongbo Zhai Xuezheng Wang Mengyao Wang Jianghua Zhao Jianguo 《气象与环境研究:英文版》 CAS 2019年第1期46-48,51共4页
In order to study the effect of reed and cattail on the bacterial community structure of surface sediments of wetland in winter, high-throughput sequencing technology was used to analyze the bacterial composition. The... In order to study the effect of reed and cattail on the bacterial community structure of surface sediments of wetland in winter, high-throughput sequencing technology was used to analyze the bacterial composition. The results showed that 134 228 high-quality gene sequences were obtained by high-throughput sequencing in sediments of natural wetland in winter, and there were 13 866 OTUs, in which the number of OUT in sediments of reed wetland and cattail wetland was 6 677 and 7 189 respectively. The richness and diversity of bacterial communities in sediments of cattail wetland in winter were higher than those in sediments of reed wetland. Relative abundance of Proteobacteria in sediments of reed wetland and cattail wetland was 0.54 and 0.52, respectively. The dominant classes of bacteria were Betaproteobacteria, Deltaproteobacteria, Gammaproteobacteria and Epsilonproteobacteria, which belonged to gram-negative bacteria, indicating that the rhizosphere of wetland was easier to enrich gram-negative bacteria. Under the genus level, the dominant bacteria in the sediments were not obvious. 展开更多
关键词 HIGH-THROUGHPUT SEQUENCING Bacterial community SEDIMENT WINTER UPSTREAM ecological WETLAND of Guanting Reservoir
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The efficacy and safety of besifloxacin for acute bacterial conjunctivitis: a Meta-analysis
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作者 Jun-Jie Wang Xin-Yi Gao +1 位作者 Hong-Zhuo Li Shan-Shuang Du 《国际眼科杂志:英文版》 SCIE CAS 2019年第6期1027-1036,共10页
AIM: To evaluate the relative efficacy and safety of besifloxacin for treatment of acute bacterial conjunctivitis.METHODS: A comprehensive search in PubMed, EMBASE Web of Science, Cochrane Central Database and CNKI wa... AIM: To evaluate the relative efficacy and safety of besifloxacin for treatment of acute bacterial conjunctivitis.METHODS: A comprehensive search in PubMed, EMBASE Web of Science, Cochrane Central Database and CNKI was undertaken for randomized controlled trials(RCTs) comparing besifloxacin with other treatments or placebo. The primary outcome measures were clinical resolution, rates of bacterial eradication, individual clinical outcomes, cure rates, and bacterial eradication rates of different kinds of pathogens. Safety outcomes were the number of adverse effects(AEs). The final search was performed on August 2018.RESULTS: Eight RCTs were included. Five studies compared the efficacy and safety of besifloxacin with placebo, 2 studies compared besifloxacin with moxifloxacin, and 1 study compared besifloxacin with gatifloxacin. A total of 3105 patients met the inclusion criteria. Besifloxacin presented higher efficacy and safety than did placebo in clinical resolution, rates of bacterial eradication, individual clinical outcomes, cure rates, bacterial eradication rates of different kinds of pathogens and the number of AEs. There was no significant difference between besifloxacin and moxifloxacin or gatifloxacin in the comparison items mentioned above.CONCLUSION: Besifloxacin is highly effective and safe for treatment of acute bacterial conjunctivitis. Further comparative trials regarding the effect of besifloxacin for treatment of acute bacterial conjunctivitis will aid in treatment decisions. 展开更多
关键词 besifloxacin ACUTE BACTERIAL CONJUNCTIVITIS META-ANALYSIS RANDOMIZED CONTROLLED trials
Effects of vegetation on the structure and diversity of soil bacterial communities in the Arctic tundra 预览
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作者 MA Yue WANG Nengfei +5 位作者 WANG Shuang HAN Wenbing LIU Jie YU Yong GUO Li YANG Guanpin 《极地科学进展:英文版》 CSCD 2019年第2期139-148,共10页
The relatively simple vegetation of the Arctic tundra provides an ideal site in which to study the relationships between plants, bacterial communities and soil chemistry. Here, results of 16S rRNA gene sequencing of s... The relatively simple vegetation of the Arctic tundra provides an ideal site in which to study the relationships between plants, bacterial communities and soil chemistry. Here, results of 16S rRNA gene sequencing of secondary Arctic brown soils collected from underneath colonies of Dryasoctopetala, Luzulaconfusa and Bistortavivipara in the Arctic tundra near Ny-Alesund, Svalbard, Norway, reveal significant differences in bacterial communities related to soil environmental properties. Redundancy analysis shows that all measured geochemical factors were significant in structuring microbiomes, with strong correlations related to soil pH and organic matter contents. Vegetation is likely to affect the physical and chemical properties of the soil, which in turn affects the bacterial community and composition of the soil. 展开更多
关键词 ARCTIC TUNDRA ARCTIC SOIL VEGETATION BACTERIAL community chemical property
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痰热清注射液联合亚胺培南和头孢哌酮/舒巴坦对广泛耐药肺炎克雷伯菌体外抑菌研究 预览
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作者 马冬梅 陶庆春 齐宏伟 《国际检验医学杂志》 CAS 2019年第1期8-11,15共5页
目的探讨痰热清注射液联合亚胺培南和头孢哌酮/舒巴坦对广泛耐药肺炎克雷伯菌的体外联合抑菌效果。方法应用肉汤稀释法测定痰热清注射液联合亚胺培南和头孢哌酮/舒巴坦对30株广泛耐药肺炎克雷伯菌的最低抑菌浓度(MIC),统计单独及联合用... 目的探讨痰热清注射液联合亚胺培南和头孢哌酮/舒巴坦对广泛耐药肺炎克雷伯菌的体外联合抑菌效果。方法应用肉汤稀释法测定痰热清注射液联合亚胺培南和头孢哌酮/舒巴坦对30株广泛耐药肺炎克雷伯菌的最低抑菌浓度(MIC),统计单独及联合用药的MIC分布,计算部分抑菌浓度(FIC)指数,判定联合用药的抑菌效果。FIC指数<0.5为协同作用,0.5~<1为相加作用,1~<2为无关作用,≥2为拮抗作用。结果痰热清注射液联合亚胺培南的FIC指数<0.5为43.3%,0.5~<1为30.0%,1~<2为20.0%,≥2为6.7%;痰热清注射液联联合头孢哌酮/舒巴坦的FIC指数<0.5为0,0.5~<1为13.3%,1~<2为33.3%,≥2为53.3%。结论痰热清注射液联合头孢哌酮/舒巴坦对广泛耐药肺炎克雷伯菌多表现为无关作用和拮抗作用;痰热清注射液联合亚胺培南多表现为协同作用和相加作用,对广泛耐药肺炎克雷伯菌的体外联合抑菌作用较好。 展开更多
关键词 微生物敏感性试验 克雷伯菌 肺炎 抗药性 细菌 头孢哌酮/舒巴坦 亚胺培南 痰热清注射液
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非特异性诊断指标对细菌性晚发型新生儿败血症的诊断意义
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作者 赵万建 冯月华 +1 位作者 李军华 曹素芬 《中国基层医药》 CAS 2019年第16期1960-1964,共5页
目的探讨非特异性诊断指标对晚发型细菌性新生儿败血症的诊断意义。方法选取细菌性晚发型新生儿败血症患儿55例为败血症组,以67例同期非感染新生儿为对照组,对白细胞、血小板数、C反应蛋白进行对比分析,同时探讨中性粒细胞百分比、平均... 目的探讨非特异性诊断指标对晚发型细菌性新生儿败血症的诊断意义。方法选取细菌性晚发型新生儿败血症患儿55例为败血症组,以67例同期非感染新生儿为对照组,对白细胞、血小板数、C反应蛋白进行对比分析,同时探讨中性粒细胞百分比、平均血小板体积对晚发型细菌性新生儿败血症的诊断价值。结果细菌性晚发型新生儿败血症时,C反应蛋白灵敏度(41.8%)、特异度(95.6%)、阳性预测值(92.0%)高;而白细胞、血小板数灵敏度(18.2%、10.9%)、阳性预测值(71.4%、85.7%)低,但特异度(94.0%、98.5%)高;中性粒细胞百分比(62.01±22.16)、平均血小板体积(11.60±0.98)高于对照组[(36.58±11.06)、(10.88±0.95)],差异均有统计学意义(P<0.05)。结论C反应蛋白对细菌性晚发型新生儿败血症的诊断意义更大,中性粒细胞百分比、平均血小板体积有一定的诊断价值。 展开更多
关键词 败血症 细菌性 晚发型 白细胞 血小板数 C反应蛋白 中性粒细胞 血小板体积 婴儿 新生
OsBGLU19 and OsBGLU23 regulate disease resistance to bacterial leaf streak in rice 预览
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作者 LI Bei-bei LIU Ying-gao +4 位作者 WU Tao WANG Ji-peng XIE Gui-rong CHU Zhao-hui DING Xin-hua 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第6期1199-1210,共12页
β-Glucosidase belongs to the glycoside hydrolase I family,which is widely present in multiple species and responds to various biotic and abiotic stresses.In rice,whetherβ-glucosidase is involved in the interaction b... β-Glucosidase belongs to the glycoside hydrolase I family,which is widely present in multiple species and responds to various biotic and abiotic stresses.In rice,whetherβ-glucosidase is involved in the interaction between plants and microorganisms is not clear.In this study,we found that the expression of several genes encodingβ-glucosidases,including OsBGLU19 and OsBGLU23,were upregulated after inoculation with Xanthomonas oryzae pv.oryzicola(Xoc)and downregulated after inoculation with X.oryzae pv.oryzae(Xoo).The respective insertion mutants of OsBGLU19 and OsBGLU23,bglu19 and bglu23,were more susceptible to Xoc infection.The expression of OsAOS2,a key gene in the jasmonic acid signal pathway,was dramatically downregulated after inoculation with Xoc in the bglu19 and bglu23 mutants.Simultaneously,the expression of downstream disease resistance-related genes,such as OsPR1a,OsPR5 and a key transcription factors OsWRKY72 were obviously downregulated.The resistance mediated by OsBGLU19 and OsBGLU23 to bacterial leaf streak is related to disease resistance-related genes above mentioned. 展开更多
关键词 Β-GLUCOSIDASE OsBGLU19 OsBGLU23 ORYZA SATIVA bacterial leaf STREAK
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牙龈卟啉单胞菌中与侵袭力相关的毒力因子的研究进展
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作者 冯莹 李红 刘怡 《中华口腔医学杂志》 CAS CSCD 北大核心 2019年第3期200-204,共5页
牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)是革兰阴性专性厌氧菌,它能侵入宿主细胞并在其内继续发挥致病作用,Pg的侵袭性对疾病的发生和发展至关重要,一直以来都是研究热点。在细菌侵袭宿主的过程中,Pg通过特异性黏附素与宿主细胞... 牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)是革兰阴性专性厌氧菌,它能侵入宿主细胞并在其内继续发挥致病作用,Pg的侵袭性对疾病的发生和发展至关重要,一直以来都是研究热点。在细菌侵袭宿主的过程中,Pg通过特异性黏附素与宿主细胞相应配体结合,激活宿主细胞内多种信号传导途径,启动细菌内化,其中Pg的多种毒力因子,如菌毛、蛋白酶、血凝素、囊泡等在侵袭过程中发挥了重要作用。本综述总结了近年来与Pg侵袭力相关的毒力因子的研究进展,为进一步阐明Pg的致病机制以及相关疾病的防治提供思路。 展开更多
关键词 紫单胞菌 纤毛 细菌 血凝素类 侵袭力
基于16S rRNA基因序列对不同土壤细菌群落的差异性分析 预览
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作者 宋国庆 李辉 +4 位作者 马克 赵雪莹 沈忆文 谢建辉 周怀谷 《法医学杂志》 CAS CSCD 2019年第2期187-193,共7页
目的研究不同土壤细菌群落的结构和差异,探索16S rRNA基因测序技术在不同土壤鉴定中的有效性。方法采集上海市7个地区的土壤样本,提取土壤细菌基因组DNA,采用高通量测序技术对16S rRNA基因序列高变区片段进行测序,测序结果通过生物信息... 目的研究不同土壤细菌群落的结构和差异,探索16S rRNA基因测序技术在不同土壤鉴定中的有效性。方法采集上海市7个地区的土壤样本,提取土壤细菌基因组DNA,采用高通量测序技术对16S rRNA基因序列高变区片段进行测序,测序结果通过生物信息学软件量化或可视化后,比较分析草地、树林和沙滩3类土壤样本间细菌群落多样性和丰度的差异。结果草地、树林和沙滩土壤样本细菌群落的alpha多样性指数差异具有统计学意义,三类土壤细菌群落的物种相对丰度和多样性差异明显,草地土壤中酸杆菌门相对丰度较高,树林土壤中变形菌门相对丰度较高,沙滩土壤中放线菌门相对丰度较高。但人工草地、天然草地和工业区草地土壤细菌群落差异无统计学意义。结论基于16S rRNA基因序列测序可以有效区分不同类别土壤,该方法有望为刑事案件第一犯罪现场判读提供线索。 展开更多
关键词 法医病理学 法医遗传学 土壤微生物学 DNA 细菌 RNA 核糖体 16S 生物多样性 高通量测序
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