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Applications of stem cells and bioprinting for potential treatment of diabetes 预览
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作者 Shweta Anil Kumar Monica Delgado +1 位作者 Victor E Mendez Binata Joddar 《世界干细胞杂志:英文版(电子版)》 2019年第1期13-32,共20页
Currently,there does not exist a strategy that can reduce diabetes and scientists are working towards a cure and innovative approaches by employing stem cellbased therapies.On the other hand,bioprinting technology is ... Currently,there does not exist a strategy that can reduce diabetes and scientists are working towards a cure and innovative approaches by employing stem cellbased therapies.On the other hand,bioprinting technology is a novel therapeutic approach that aims to replace the diseased or lostβ-cells,insulin-secreting cells in the pancreas,which can potentially regenerate damaged organs such as the pancreas.Stem cells have the ability to differentiate into various cell lines including insulinproducing cells.However,there are still barriers that hamper the successful differentiation of stem cells intoβ-cells.In this review,we focus on the potential applications of stem cell research and bioprinting that may be targeted towards replacing theβ-cells in the pancreas and may offer approaches towards treatment of diabetes.This review emphasizes on the applicability of employing both stem cells and other cells in 3D bioprinting to generate substitutes for diseasedβ-cells and recover lost pancreatic functions.The article then proceeds to discuss the overall research done in the field of stem cell-based bioprinting and provides future directions for improving the same for potential applications in diabetic research. 展开更多
关键词 BIOPRINTING Tissue engineering Pluripotent STEM CELLS Mesenchymal STEM CELLS HUMAN embryonic STEM Adult HUMAN liver CELLS β-cells Islet CELLS Biomaterials Bioink STEM cell DIABETES
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Porcine pluripotent stem cells: progress, challenges and prospects
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作者 Jianyong HAN Yi-Liang MIAO +7 位作者 Jinlian HUA Yan LI Xue ZHANG Jilong ZHOU Na LI Ying ZHANG Jinying ZHANG Zhonghua LIU 《农业科学与工程前沿:英文版》 2019年第1期8-27,共20页
Pluripotent stem cells(PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a ver... Pluripotent stem cells(PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a very suitable model for the studies of human diseases, drugs screening, regenerative medicine and developmental biology research. Pigs are considered as an ideal model for preclinical development of human xenotransplantation, therapeutic approaches and regenerative medicine because of their size and physiological similarity to humans. However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcine PSCs hinders progress in these biotechnologies. In this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotency, the scienti?c and technical questions arising from these studies. We also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generating naive porcine PSCs and animal breeding. 展开更多
关键词 EMBRYONIC GERM CELLS EMBRYONIC STEM CELLS induced PLURIPOTENT STEM CELLS PIGS PLURIPOTENT STEM CELLS
Current status and future prospects of stem cell therapy in Alzheimer’s disease 预览
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作者 Fu-Qiang Zhang Jin-Lan Jiang +3 位作者 Jing-Tian Zhang Han Niu Xue-Qi Fu Lin-Lin Zeng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期242-250,共9页
Alzheimer’s disease is a common progressive neurodegenerative disorder, pathologically characterized by the presence of β-amyloid plaques and neurofibrillary tangles. Current treatment approaches using drugs only al... Alzheimer’s disease is a common progressive neurodegenerative disorder, pathologically characterized by the presence of β-amyloid plaques and neurofibrillary tangles. Current treatment approaches using drugs only alleviate the symptoms without curing the disease, which is a serious issue and influences the quality of life of the patients and their caregivers. In recent years, stem cell technology has provided new insights into the treatment of neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. Currently, the main sources of stem cells include neural stem cells, embryonic stem cells, mesenchymal stem cells, and induced pluripotent stem cells. In this review, we discuss the pathophysiology and general treatment of Alzheimer’s disease, and the current state of stem cell transplantation in the treatment of Alzheimer’s disease. We also assess future challenges in the clinical application and drug development of stem cell transplantation as a treatment for Alzheimer’s disease. 展开更多
关键词 Alzheimer's disease Β-AMYLOID drug development embryonic STEM CELLS induced pluripotent STEM CELLS mesenchymal STEM CELLS nerve REGENERATION NEURAL REGENERATION NEURAL STEM CELLS NEURODEGENERATIVE disorders STEM cell therapy
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Predicting differentiation potential of human pluripotent stem cells:Possibilities and challenges 预览
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作者 Li-Ping Liu Yun-Wen Zheng 《世界干细胞杂志:英文版(电子版)》 2019年第7期375-382,共8页
The capability of human pluripotent stem cell(hPSC)lines to propagate indefinitely and differentiate into derivatives of three embryonic germ layers makes these cells be powerful tools for basic scientific research an... The capability of human pluripotent stem cell(hPSC)lines to propagate indefinitely and differentiate into derivatives of three embryonic germ layers makes these cells be powerful tools for basic scientific research and promising agents for translational medicine.However,variations in differentiation tendency and efficiency as well as pluripotency maintenance necessitate the selection of hPSC lines for the intended applications to save time and cost.To screen the qualified cell lines and exclude problematic cell lines,their pluripotency must be confirmed initially by traditional methods such as teratoma formation or by highthroughput gene expression profiling assay.Additionally,their differentiation potential,particularly the lineage-specific differentiation propensities of hPSC lines,should be predicted in an early stage.As a complement to the teratoma assay,RNA sequencing data provide a quantitative estimate of the differentiation ability of hPSCs in vivo.Moreover,multiple scorecards have been developed based on selected gene sets for predicting the differentiation potential into three germ layers or the desired cell type many days before terminal differentiation.For clinical application of hPSCs,the malignant potential of the cells must also be evaluated.A combination of histologic examination of teratoma with quantitation of gene expression data derived from teratoma tissue provides safety-related predictive information by detecting immature teratomas,malignancy marker expression,and other parameters.Although various prediction methods are available,distinct limitations remain such as the discordance of results between different assays and requirement of a long time and high labor and cost,restricting their wide applications in routine studies.Therefore,simpler and more rapid detection assays with high specificity and sensitivity that can be used to monitor the status of hPSCs at any time and fewer targeted markers that are more specific for a given desired cell type are urgently needed. 展开更多
关键词 Human PLURIPOTENT STEM CELLS Induced PLURIPOTENT STEM CELLS Embryonic STEM CELLS DIFFERENTIATION POTENTIAL Prediction Pluripotency Malignant POTENTIAL EMBRYOID bodies Lineage-specific DIFFERENTIATION Teratoma
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MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronallike cells by Schwann cell-conditioned medium 预览
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作者 Zhi-Jian Wei Bao-You Fan +9 位作者 Yang Liu Han Ding Hao-Shuai Tang Da-Yu Pan Jia-Xiao Shi Peng-Yuan Zheng Hong-Yu Shi Heng Wu Ang Li Shi-Qing Feng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第8期1462-1469,共8页
Bone marrow-derived mesenchymal stem cells differentiate into neurons under the induction of Schwann cells. However, key microRNAs and related pathways for differentiation remain unclear. This study screened and ident... Bone marrow-derived mesenchymal stem cells differentiate into neurons under the induction of Schwann cells. However, key microRNAs and related pathways for differentiation remain unclear. This study screened and identified differentially expressed microRNAs in bone marrow- derived mesenchymal stem cells induced by Schwann cell-conditioned medium, and explored targets and related pathways involved in their differentiation into neuronal-like cells. Primary bone marrow-derived mesenchymal stem cells were isolated from femoral and tibial bones, while primary Schwann cells were isolated from bilateral saphenous nerves. Bone marrow-derived mesenchymal stem cells were cultured in unconditioned (control group) and Schwann cell-conditioned medium (bone marrow-derived mesenchymal stem cell + Schwann cell group). Neuronal differentiation of bone marrow-derived mesenchymal stem cells induced by Schwann cell-conditioned medium was observed by time-lapse imaging. Upon induction, the morphology of bone marrow-derived mesencaymal stem cells changed into a neural shape with neurites. Results of quantitative reverse transcription-polymerase chain reaction revealed that nestin mRNA expression was upregulated from 1 to 3 days and downregulated from 3 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group. Compared with the control group, microtubule-associated protein 2 mRNA expression gradually increased from 1 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group. After 7 days of induction, microRNA analysis iden:ified 83 significantly differentially expressed microRNAs between the two groups. Gene Ontology analysis indicated enrichment of microRNA target genes for neuronal projection development, regulation of axonogenesis, and positive regulation of cell proliferation. Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated that Hippo, Wnt, transforming growth factor-beta, and Hedgehog signaling pathv/ays were potentially associated with neural differentiation of b 展开更多
关键词 nerve REGENERATION MICRORNA analysis bone marrow-derived mesenchymal stem cells: Schwann CELLS neuronal-like CELLS neuronal differentiation Gene Ontology analysis Hippo SIGNALING PATHWAY Wnt SIGNALING PATHWAY transforming growth FACTOR-BETA SIGNALING PATHWAY Hedgehog SIGNALING PATHWAY neural REGENERATION
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Perspective Material for Photoenergetics on the Basis of Silicon with Binary Elementary Cells 预览
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作者 M.K. Bakhadyrhanov U.X. Sodikov +2 位作者 Kh.M. Iliev S.A. Tachilin Tuerdi Wumaier 《材料物理与化学》 2019年第1期89-95,共7页
The paper proposes a scientifically grounded - principally new approach to managing the fundamental parameters of the basic material of electronic engineering as like silicon. The essense of the proposed approach is t... The paper proposes a scientifically grounded - principally new approach to managing the fundamental parameters of the basic material of electronic engineering as like silicon. The essense of the proposed approach is that the formation of binary elementary cells in the silicon lattice involving elements III (B, Al, Ga, Zn) and V (P, As, Sb) groups in the form of Si2GaAs, Si2GaSb, etc., taking into account electrical and chemical parameters of these impurity atoms, as well as their diffusion parameters in Si, are determined by the most suitable pairs of atoms of groups III and V that allow obtaining silicon with the necessary composition and structure of binary elementary cells, as well as their more complex associations, up to the formation of nanocrystals of semiconductor connections AIIIBV. It is shown that by controlling the composition and structure, as well as the concentration of binary elementary cells, it is possible to significantly expand the spectral sensitivity of silicon, both in the IR and hλ> Eg directions. The formation of nanoclusters of AIIIBV semiconductor compounds in the silicon lattice significantly changes the emissivity of the material. It is established that the successive diffusion of elements of groups III and V in silicon and additional low-temperature annealing under certain thermodynamic conditions makes it possible to ensure the maximum participation of the impurity atoms introduced in the formation of binary elementary cells. It is shown that silicon with binary elementary cells involving atoms of groups III and V is a new class of semiconductor material with unique functionality for modern optoelectronics and photoenergetics. 展开更多
关键词 Semiconductor Binary clusters Muticascade PV CELLS Elementary CELLS SELF-ORGANIZATION Self-Structure Nanostructure Nanocrystal Photosensivity Combinations MULTISTAGE PV CELLS
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Immune suppression in chronic hepatitis B infection associated liver disease: A review 预览
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作者 Tian-Yang Li Yang Yang +1 位作者 Guo Zhou Zheng-Kun Tu 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第27期3527-3537,共11页
Hepatitis B virus(HBV) infection is one the leading risk factors for chronic hepatitis, liver fibrosis, cirrhosis and hepatocellular cancer(HCC), which are a major global health problem. A large number of clinical stu... Hepatitis B virus(HBV) infection is one the leading risk factors for chronic hepatitis, liver fibrosis, cirrhosis and hepatocellular cancer(HCC), which are a major global health problem. A large number of clinical studies have shown that chronic HBV persistent infection causes the dysfunction of innate and adaptive immune response involving monocytes/macrophages, dendritic cells, natural killer(NK) cells, T cells. Among these immune cells, cell subsets with suppressive features have been recognized such as myeloid derived suppressive cells(MDSC),NK-reg, T-reg, which represent a critical regulatory system during liver fibrogenesis or tumourigenesis. However, the mechanisms that link HBVinduced immune dysfunction and HBV-related liver diseases are not understood.In this review we summarize the recent studies on innate and adaptive immune cell dysfunction in chronic HBV infection, liver fibrosis, cirrhosis, and HCC, and further discuss the potential mechanism of HBV-induced immunosuppressive cascade in HBV infection and consequences. It is hoped that this article will help ongoing research about the pathogenesis of HBV-related hepatic fibrosis and HBV-related HCC. 展开更多
关键词 Hepatitis B virus Hepatocellular carcinoma Liver FIBROSIS REGULATORY T CELLS REGULATORY natural KILLER CELLS DENDRITIC CELLS MONOCYTES
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Immune response pattern varies with the natural history of chronic hepatitis B 预览
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作者 Wen-Tao Wang Xue-Qi Zhao +9 位作者 Gui-Ping Li Yi-Zhi Chen Lin Wang Mei-Fang Han Wei-Na Li Tao Chen Guang Chen Dong Xu Qin Ning Xi-Ping Zhao 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第16期1950-1963,共14页
BACKGROUND Chronic hepatitis B is a highly heterogeneous disease that can be divided into four phases: Immune tolerant (IT), immune active (IA), inactive carrier (IC) and hepatitis B envelope antigen (HBeAg)-negative ... BACKGROUND Chronic hepatitis B is a highly heterogeneous disease that can be divided into four phases: Immune tolerant (IT), immune active (IA), inactive carrier (IC) and hepatitis B envelope antigen (HBeAg)-negative hepatitis (ENEG). AIM To investigate the immune status of natural killer (NK) and T cells in different phases of chronic hepatitis B. METHODS The frequency, phenotype and function of circulating NK cells, as well as nonantigen-specific and hepatitis B virus (HBV)-specific T cell responses were detected by flow cytometry in healthy and HBV-infected subjects. RESULTS The ability of NK cells to produce IFN-γ was markedly attenuated in HBVinfected patients overall but was less compromised in IC patients. Patients in the IT and IA phases also displayed significantly lower TNF-α production compared to healthy subjects. NK cells were phenotypically activated in the IA and ENEG phases, as evidenced by the upregulation of NKp44 in CD56bright NK cells and CD69 in CD56dim NK cells. Furthermore, global T-cells from the ENEG phase displayed a proinflammatory cytokine profile with upregulated IFN-γ and TNF-α expression, while this profile was suppressed in IT and IA patients. Finally, core and S antigen-specific T cell responses were significantly stronger after in vitro expansion in the IC phase compared to other phases. CONCLUSION Our findings demonstrate the changes in immune response pattern during the natural history of HBV infection. Both NK and T cells are functionally impaired in the IT and IA phases. With the spontaneous clearance of HBeAg and hepatitis B surface antigen decline, NK cell cytokine production and HBV-specific T responses are partially restored in IC phase, and the ENEG phase is dominated by nonantigen-specific T cell responses. 展开更多
关键词 Chronic HEPATITIS HEPATITIS B virus NATURAL KILLER CELLS Global-T CELLS Virusspecific T CELLS NATURAL HISTORY Heterogeneity
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Transfer of mitochondria from mesenchymal stem cells derived from induced pluripotent stem cells attenuates hypoxia-ischemia-induced mitochondrial dysfunction in PC12 cells 预览
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作者 Yan Yang Gen Ye +5 位作者 Yue-Lin Zhang Hai-Wei He Bao-Qi Yu Yi-Mei Hong Wei You Xin Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期464-472,共9页
Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury.Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology,the mechanisms are not fully... Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury.Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology,the mechanisms are not fully understood.To address this issue,we first co-cultured 1.5×10^5 PC12 cells with mesenchymal stem cells that were derived from induced pluripotent stem cells at a ratio of 1:1,and then intervened with cobalt chloride(CoCl2)for 24 hours.Reactive oxygen species in PC12 cells was measured by Mito-sox.Mitochondrial membrane potential(ΔΨm)in PC12 cells was determined by JC-1 staining.Apoptosis of PC12 cells was detected by terminal deoxynucleotidal transferase-mediated dUTP nick end-labeling staining.Mitochondrial morphology in PC12 cells was examined by transmission electron microscopy.Transfer of mitochondria from the mesenchymal stem cells derived from induced pluripotent stem cells to damaged PC12 cells was measured by flow cytometry.Mesenchymal stem cells were induced from pluripotent stem cells by lentivirus infection containing green fluorescent protein in mitochondria.Then they were co-cultured with PC12 cells in Transwell chambers and treated with CoCl2 for 24 hours to detect adenosine triphosphate level in PC12 cells.CoCl2-induced PC12 cell damage was dose-dependent.Co-culture with mesenchymal stem cells significantly reduced apoptosis and restoredΔΨm in the injured PC12 cells under CoCl2 challenge.Co-culture with mesenchymal stem cells ameliorated mitochondrial swelling,the disappearance of cristae,and chromatin margination in the injured PC12 cells.After direct co-culture,mitochondrial transfer from the mesenchymal stem cells stem cells to PC12 cells was detected via formed tunneling nanotubes between these two types of cells.The transfer efficiency was greatly enhanced in the presence of CoCl2.More importantly,inhibition of tunneling nanotubes partially abrogated the beneficial effects of mesenchymal stem cells on CoCl2-induced PC12 cell injury.Mesenchymal stem cells reduced CoCl2-induced 展开更多
关键词 apoptosis brain injury HYPOXIA-ISCHEMIA induced pluripotent stem cells mesenchymal stem cells mitochondrial membrane potential mitochondrial transfer PC12 cells tunneling nanotubes
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银屑病发病机制研究新进展 预览
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作者 王超颖 张慧敏(指导) 《世界最新医学信息文摘》 2019年第60期28-31,共4页
目前,银屑病在全球范围内的发病率大约是0.1%-3%,该病属于炎症性慢性皮肤病。研究证实银屑病的发病原因主要与t细胞以及表皮细胞介导的慢性炎症有很大关系。临床上对于银屑病治疗主要以tnf-α拮抗剂生物制剂为主要药物,如英夫利西单抗... 目前,银屑病在全球范围内的发病率大约是0.1%-3%,该病属于炎症性慢性皮肤病。研究证实银屑病的发病原因主要与t细胞以及表皮细胞介导的慢性炎症有很大关系。临床上对于银屑病治疗主要以tnf-α拮抗剂生物制剂为主要药物,如英夫利西单抗、阿达木单抗以及依那西普等,尽管此类药物对于银屑病的治疗存在诸多优点,但是仍然有半数以上患者对药物副作用无法耐受或者治疗效果不甚理想。近年来,最新研究表明阻断il-17与il-23受体来治疗银屑病获得了十分理想的效果,il-17受体拮抗剂主要有ixekizumab、brodalumab以及secukinumab三种,il-23受体拮抗剂主要有risankizumab、tildrakizumab以及guselkuman三种。本文将针对与银屑病发病机制相关的t细胞亚群、表皮细胞以及最新治疗该病的生物制剂作如下综述。 展开更多
关键词 银屑病 T细胞 表皮细胞 il-17受体拮抗剂
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Physiological and pathological effects of amyloid-β species in neural stem cell biology 预览
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作者 Adela Bernabeu-Zornoza Raquel Coronel +3 位作者 Charlotte Palmer María Monteagudo Alberto Zambrano Isabel Liste 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2035-2042,共8页
Although amyloid-β peptide is considered neurotoxic, it may mediate several physiological processes during embryonic development and in the adult brain. The pathological function of amyloid-β peptide has been extens... Although amyloid-β peptide is considered neurotoxic, it may mediate several physiological processes during embryonic development and in the adult brain. The pathological function of amyloid-β peptide has been extensively studied due to its implication in Alzheimer’s disease, but its physiological function remains poorly understood. Amyloid-β peptide can be detected in non-aggregated (monomeric) and aggregated (oligomeric and fibrillary) forms. Each form has different cytotoxic and/or physiological properties, so amyloid-β peptide and its role in Alzheimer’s disease need to be studied further. Neural stem cells and neural precursor cells are good tools for the study on neurodegenerative diseases and can provide future therapeutic applications in diseases such as Alzheimer’s disease. In this review, we provide an outline of the effects of amyloid-β peptide, in monomeric and aggregated forms, on the biology of neural stem cells/neural precursor cells, and discuss the controversies. We also describe the possible molecular targets that could be implicated in these effects, especially GSK3β. A better understanding of amyloid-β peptide (both physiological and pathological), and the signaling pathways involved are essential to advance the field of Alzheimer’s disease. 展开更多
关键词 amyloid-β peptide NEURAL stem CELLS NEURAL PROGENITOR CELLS Alzheimer's disease AMYLOID precursor protein toxicity neurogenesis GLIOGENESIS GSK3β
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Ni(OH)2-Ni/C for hydrogen oxidation reaction in alkaline media 预览
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作者 Yangxin Pan Gaohe Hu +2 位作者 Juntao Lu Li Xiao Lin Zhuang 《能源化学:英文版》 SCIE EI CAS CSCD 2019年第2期111-115,共5页
The development of the hydrogen electrode is vital for the application of alkaline polymer electrolyte fuel cells(APEFCs).In this study,a series of Ni(OH)2 decorated Ni/C catalysts(Ni(OH)2-Ni/C) were prepared by a thr... The development of the hydrogen electrode is vital for the application of alkaline polymer electrolyte fuel cells(APEFCs).In this study,a series of Ni(OH)2 decorated Ni/C catalysts(Ni(OH)2-Ni/C) were prepared by a three-step electrochemical treatment of Ni/C.The existence of Ni(OH)2 was demonstrated by X-ray photoelectron spectroscopy(XPS),and the surface molar ratio of Ni(OH)2/Ni of the samples was estimated via an electrochemical method.The HOR catalytic activity of the catalysts was evaluated by a rotation disk electrode(RDE) method,and a 'volcano plot' was established between the HOR exchange current(j0) and the surface molar ratio of Ni(OH)2/Ni.On top of the 'volcano',the surface molar ratio of Ni(OH)2/Ni is1.1:1,the j0 of which was 6.8 times of that of Ni/C.The stability of the samples toward HOR was evaluated to be good.Our study added a systematic experimental evidence to the HOR research,showing that the HOR catalytic activity of Ni can be deliberately controlled via decoration of Ni(OH)2,which may help understanding the HOR mechanism on Ni. 展开更多
关键词 Hydrogen oxidation reaction FUEL CELLS Alkaline polymer ELECTROLYTE FUEL CELLS Nickel VOLCANO PLOT
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Induced pluripotent stem cells from Huntington’s disease patients:a promising approach to define and correct disease-related alterations 预览
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作者 Azra Fatima Ricardo Gutiérrez-Garcia David Vilchez 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期769-770,共2页
Adult somatic cells such as skin or blood cells from either health donors or patients can be reprogrammed into induced pluripotent stem cells(iPSCs).Given their unlimited self-renewal and differentiation capacities,iP... Adult somatic cells such as skin or blood cells from either health donors or patients can be reprogrammed into induced pluripotent stem cells(iPSCs).Given their unlimited self-renewal and differentiation capacities,iPSCs are an invaluable resource to generate terminally differentiated cells.Thus,iPSCs can facilitate the study of human diseases and drug screening,holding great promise for regenerative medicine.Another significant advantage of iPSC disease-modeling is that normal and mutant proteins are expressed at endogenous levels.In addition,subtle phenotypes and the effects of genetic background variations can be assessed by comparison between iPSC lines obtained from different patients and healthy donors as well as isogenic lines,in which disease-related mutations are corrected. 展开更多
关键词 ADULT SOMATIC CELLS skin or blood CELLS Huntington’s DISEASE PATIENTS
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Gene expression changes in dorsal root ganglia following peripheral nerve injury:roles in inflammation,cell death and nociception 预览
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作者 Sarah L. Martin Adam J. Reid +2 位作者 Alexei Verkhratsky Valerio Magnaghi Alessandro Faroni 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期939-947,共9页
Subsequent to a peripheral nerve injury,there are changes in gene expression within the dorsal root ganglia in response to the damage.This review selects factors which are well-known to be vital for inflammation,cell ... Subsequent to a peripheral nerve injury,there are changes in gene expression within the dorsal root ganglia in response to the damage.This review selects factors which are well-known to be vital for inflammation,cell death and nociception,and highlights how alterations in their gene expression within the dorsal root ganglia can affect functional recovery.The majority of studies used polymerase chain reaction within animal models to analyse the dynamic changes following peripheral nerve injuries.This review aims to highlight the factors at the gene expression level that impede functional recovery and are hence are potential targets for therapeutic approaches.Where possible the experimental model,specific time-points and cellular location of expression levels are reported. 展开更多
关键词 Gene expression polymerase chain reaction dorsal root GANGLIA INFLAMMATION NOCICEPTION cell death peripheral NERVE injury Schwann CELLS satellite GLIAL CELLS NERVE regeneration
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Dysfunctional stem and progenitor cells impair fracture healing with age 预览
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作者 Diane R Wagner Sonali Karnik +10 位作者 Zachary J Gunderson Jeffery J Nielsen Alanna Fennimore Hunter J Promer Jonathan W Lowery M Terry Loghmani Philip S Low Todd O McKinley Melissa A Kacena Matthias Clauss Jiliang Li 《世界干细胞杂志:英文版(电子版)》 2019年第6期281-296,共16页
Successful fracture healing requires the simultaneous regeneration of both the bone and vasculature;mesenchymal stem cells (MSCs) are directed to replace the bone tissue, while endothelial progenitor cells (EPCs) form... Successful fracture healing requires the simultaneous regeneration of both the bone and vasculature;mesenchymal stem cells (MSCs) are directed to replace the bone tissue, while endothelial progenitor cells (EPCs) form the new vasculature that supplies blood to the fracture site. In the elderly, the healing process is slowed, partly due to decreased regenerative function of these stem and progenitor cells. MSCs from older individuals are impaired with regard to cell number, proliferative capacity, ability to migrate, and osteochondrogenic differentiation potential. The proliferation, migration and function of EPCs are also compromised with advanced age. Although the reasons for cellular dysfunction with age are complex and multidimensional, reduced expression of growth factors, accumulation of oxidative damage from reactive oxygen species, and altered signaling of the Sirtuin-1 pathway are contributing factors to aging at the cellular level of both MSCs and EPCs. Because of these geriatric-specific issues, effective treatment for fracture repair may require new therapeutic techniques to restore cellular function. Some suggested directions for potential treatments include cellular therapies, pharmacological agents, treatments targeting age-related molecular mechanisms, and physical therapeutics. Advanced age is the primary risk factor for a fracture, due to the low bone mass and inferior bone quality associated with aging;a better understanding of the dysfunctional behavior of the aging cell will provide a foundation for new treatments to decrease healing time and reduce the development of complications during the extended recovery from fracture healing in the elderly. 展开更多
关键词 Fracture HEALING Aging Bone Angiogenesis MESENCHYMAL STEM CELLS Endothelial PROGENITOR CELLS
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TGF-β1 promotes differentiation of hiPSC into functional smooth-muscle-like cells
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作者 姚博谦 马文韬 +2 位作者 周嘉辉 刘尚敏 林展翼 《岭南心血管病杂志:英文版》 CAS 2019年第1期44-53,共10页
Background Cell source is one of the most important constructions for tissue engineered blood vessels(TEBV). As human adult vascular cells are limited by the replicative life spans and poor collagen secretion, stem ce... Background Cell source is one of the most important constructions for tissue engineered blood vessels(TEBV). As human adult vascular cells are limited by the replicative life spans and poor collagen secretion, stem cell has become a promising cell source. Hence, we investigated the differentiation of human induced pluripotent stem cells(hiPSC) into functional smooth-muscle-like cells(SMLCs) by embryoid bodies method and explored whether transforming growth factor-β1(TGF-β1) can promote the differentiation. Methods HiPSCs were cultured in smooth muscle cell medium with or without TGF-β1 after forming embryoid bodies. The cell morphology, cell characteristics and contractility were compared after 7 days of differentiation. Real-time PCR and Western blot were used to assess the mRNA and protein expression levels of α-SMA, Calponin, SM22α, Collagen I and Collagen III. Functional contraction study was performed using carbachol. Results HiPSC could successfully differentiate into cells that were similar to typical smooth muscle cells in morphology. The expression of α-SMA, Calponin and SM22α up-regulated after induction. TGF-β1 could further up-regulated α-SMA expression.Immunofluorescence images showed that more than 80% of the hiPSC-derived SMLCs by TGF-β1 stained with smooth muscle cell markers α-SMA, SMMHC, SM22α and Calponin. Analyses of expression in collagen showed that hiPSC-derived SMLCs exhibited higher levels of Collagen I and Collagen III after induction by TGF-β1. Conclusion The hiPSC could successfully differentiate into smooth-muscle-like cells using embryoid bodies method. TGF-β1 can promote the differentiation and enhance collagen synthesis. 展开更多
关键词 induced PLURIPOTENT stem CELLS smooth-muscle-like CELLS TRANSFORMING growth factor-β1 DIFFERENTIATION COLLAGEN
Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review
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作者 Qijing LEI Qin PAN +4 位作者 Shuai YU Na LI Shulin CHEN Kuldip SIDHU Jinlian HUA 《农业科学与工程前沿:英文版》 2019年第1期28-32,共5页
Primordial germ cells(PGCs) are regarded as unipotent cells that can produce only either spermatogonia or oocytes. However, PGCs can be converted into the pluripotent state by ?rst dedifferentiation to embryonic germ ... Primordial germ cells(PGCs) are regarded as unipotent cells that can produce only either spermatogonia or oocytes. However, PGCs can be converted into the pluripotent state by ?rst dedifferentiation to embryonic germ cells and then by reprogramming to induce them to become pluripotent stem cells(iPSCs). These two stages can be completely implemented with mouse cells. However, authentic porcine iPSCs have not been established.Here, we discuss recent advances in the stem cell ?eld for obtaining iPSCs from PGCs. This knowledge will provide some clues which will contribute to the regulation of reprogramming to pluripotency in farm species. 展开更多
关键词 PIG PLURIPOTENT stem CELLS primordial GERM CELLS REPROGRAMMING
Neuroprotective effect of a dietary supplement against glutamate-induced excitotoxicity in retina
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作者 Takahiro Kurose Eriko Sugano +7 位作者 Akihisa Sugai Raki Shiraiwa Mariyo Kato Yoko Mitsuguchi Yoshihiro Takai Kitako Tabata Yoichi Honma Hiroshi Tomita 《国际眼科杂志:英文版》 SCIE CAS 2019年第8期1231-1237,共7页
AIM: To evaluate the neuroprotective effect of a dietary supplement(ClearVision EX~?;CV) against glutamateinduced excitotoxicity in retina.METHODS: We evaluated the protective effects CV on glutamate-induced cell toxi... AIM: To evaluate the neuroprotective effect of a dietary supplement(ClearVision EX~?;CV) against glutamateinduced excitotoxicity in retina.METHODS: We evaluated the protective effects CV on glutamate-induced cell toxicity of an immortalized mouse hippocampal cell line(HT-22) in vitro and N-methyl-Daspartate(NMDA) induced retinal injury in vivo. Once-daily oral administration of CV or vehicle(5% Arabic gum) was started the day before the NMDA injection and continued until the end of the study. Electroretinograms(ERGs) were recorded to evaluate the retinal function at 2 d after NMDA injection. Furthermore, a histological evaluation, Western blot analysis, and immunohistochemistry were performed for assessing the signal transduction pathway. RESULTS: HT-22 cell death was induced by the addition of glutamate and co-incubation with CV protected against it. Oral administration of CV inhibited the decrease in scotopic threshold response amplitudes induced by the intravitreal injection of NMDA and those of the thickness of the inner retinal layer in the histological evaluation. The increased phosphorylated levels of extracellular signalregulated kinase(ERK) but not cAMP response element binding protein(CREB) or Akt were observed 1 h after NMDA injection in both the vehicle-and CV-treated rats;however, p ERK activation was no more upregulated at 3 h after NMDA injection. pERK upregulation was observed in Müller cells.CONCLUSION: CV shows a protective effect against both glutamate-induced HT-22 cell death and NMDAinduced retinal damage. pERK upregulation in the Müller cells plays a key role in the protective effect of CV against glutamate-induced retinal toxicity. 展开更多
关键词 glutamate-induced toxicity retinal GANGLION CELLS HT-22 CELLS PERK
P2X7 receptor signaling during adult hippocampal neurogenesis 预览
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作者 Hannah C. Leeson Tailoi Chan-Ling +3 位作者 Michael D. Lovelace Jeremy C. Brownlie Ben J. Gu Michael W. Weible II 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1684-1694,共11页
Neurogenesis is a persistent and essential feature of the adult mammalian hippocampus.Granular neurons generated from resident pools of stem or progenitor cells provide a mechanism for the formation and consolidation ... Neurogenesis is a persistent and essential feature of the adult mammalian hippocampus.Granular neurons generated from resident pools of stem or progenitor cells provide a mechanism for the formation and consolidation of new memories.Regulation of hippocampal neurogenesis is complex and multifaceted,and numerous signaling pathways converge to modulate cell proliferation,apoptosis,and clearance of cellular debris,as well as synaptic integration of newborn immature neurons.The expression of functional P2X7 receptors in the central nervous system has attracted much interest and the regulatory role of this purinergic receptor during adult neurogenesis has only recently begun to be explored.P2X7 receptors are exceptionally versatile:in their canonical role they act as adenosine triphosphate-gated calcium channels and facilitate calcium-signaling cascades exerting control over the cell via calcium-encoded sensory proteins and transcription factor activation.P2X7 also mediates transmembrane pore formation to regulate cytokine release and facilitate extracellular communication,and when persistently stimulated by high extracellular adenosine triphosphate levels large P2X7 pores form,which induce apoptotic cell death through cytosolic ion dysregulation.Lastly,as a scavenger receptor P2X7 directly facilitates phagocytosis of the cellular debris that arises during neurogenesis,as well as during some disease states.Understanding how P2X7 receptors regulate the physiology of stem and progenitor cells in the adult hippocampus is an important step towards developing useful therapeutic models for regenerative medicine.This review considers the relevant aspects of adult hippocampal neurogenesis and explores how P2X7 receptor activity may influence the molecular physiology of the hippocampus,and neural stem and progenitor cells. 展开更多
关键词 P2X7 P2X7R adult neurogenesis NEURAL stem CELLS NEURAL PROGENITOR CELLS hippocampus SGZ calcium SIGNALING PURINERGIC SIGNALING
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Contralateral C7 transfer combined with acellular nerve allografts seeded with differentiated adipose stem cells for repairing upper brachial plexus injury in rats 预览
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作者 Jian-Tao Yang Jin-Tao Fang +3 位作者 Liang Li Gang Chen Ben-Gang Qin Li-Qiang Gu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1932-1940,共9页
Nerve grafting has always been necessary when the contralateral C7 nerve root is transferred to treat brachial plexus injury. Acellular nerve allograft is a promising alternative for the treatment of nerve defects, an... Nerve grafting has always been necessary when the contralateral C7 nerve root is transferred to treat brachial plexus injury. Acellular nerve allograft is a promising alternative for the treatment of nerve defects, and results were improved by grafts laden with differentiated adipose stem cells. However, use of these tissue-engineered nerve grafts has not been reported for the treatment of brachial plexus injury. The aim of the present study was to evaluate the outcome of acellular nerve allografts seeded with differentiated adipose stem cells to improve nerve regeneration in a rat model in which the contralateral C7 nerve was transferred to repair an upper brachial plexus injury. Differentiated adipose stem cells were obtained from Sprague-Dawley rats and transdifferentiated into a Schwann cell-like phenotype. Acellular nerve allografts were prepared from 15-mm bilateral sections of rat sciatic nerves. Rats were randomly divided into three groups: acellular nerve allograft, acellular nerve allograft + differentiated adipose stem cells, and autograft. The upper brachial plexus injury model was established by traction applied away from the intervertebral foramen with micro-hemostat forceps. Acellular nerve allografts with or without seeded cells were used to bridge the gap between the contralateral C7 nerve root and C5–6 nerve. Histological staining, electrophysiology, and neurological function tests were used to evaluate the effect of nerve repair 16 weeks after surgery. Results showed that the onset of discernible functional recovery occurred earlier in the autograft group first, followed by the acellular nerve allograft + differentiated adipose stem cells group, and then the acellular nerve allograft group;moreover, there was a significant difference between autograft and acellular nerve allograft groups. Compared with the acellular nerve allograft group, compound muscle action potential, motor conduction velocity, positivity for neurofilament and S100, diameter of regenerating axons, myelin sheath thickness, 展开更多
关键词 NERVE REGENERATION peripheral NERVE INJURY brachial plexus INJURY CONTRALATERAL C7 NERVE root acellular NERVE adipose stem CELLS Schwann CELLS tissue engineering NERVE NERVE grafting NERVE defect neural REGENERATION
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