期刊文献+
共找到8,780篇文章
< 1 2 250 >
每页显示 20 50 100
Mucosal healing progression after acute colitis in mice 预览
1
作者 Sandra Vidal-Lletjós Mireille Andriamihaja +7 位作者 Anne Blais Marta Grauso Patricia Lepage Anne-Marie Davila Claire Gaudichon Marion Leclerc Fran?ois Blachier Anna?g Lan 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第27期3572-3589,共18页
BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases. To achieve this objective, overlapping actions of complex cellular processes, such as ... BACKGROUND Mucosal healing has become a therapeutic goal to achieve stable remission in patients with inflammatory bowel diseases. To achieve this objective, overlapping actions of complex cellular processes, such as migration, proliferation, and differentiation, are required. These events are longitudinally and tightly controlled by numerous factors including a wide range of distinct regulatory proteins. However, the sequence of events associated with colon mucosal repair after colitis and the evolution of the luminal content characteristics during this process have been little studied.AIM To document the evolution of colon mucosal characteristics during mucosal healing using a mouse model with chemically-induced colitis.METHODS C57 BL/6 male mice were given 3.5% dextran sodium sulfate(DSS) in drinking water for 5 d. They were euthanized 2(day 7), 5(day 10), 8(day 13), and 23(day28) d after DSS removal. The colonic luminal environment and epithelial repair processes during the inflammatory flare and colitis resolution were analyzed with reference to a non-DSS treated control group, euthanized at day 0. Epithelial repair events were assessed histo-morphologically in combination with functional permeability tests, expression of key inflammatory and repairing factors, and evaluation of colon mucosa-adherent microbiota composition by 16 S rRNA sequencing.RESULTS The maximal intensity of colitis was concomitant with maximal alterations of intestinal barrier function and histological damage associated with goblet cell depletion in colon mucosa. It was recorded 2 d after termination of the DSStreatment, followed by a progressive return to values similar to those of control mice. Although signs of colitis were severe(inflammatory cell infiltrate, crypt disarray, increased permeability) and associated with colonic luminal alterations(hyperosmolarity, dysbiosis, decrease in short-chain fatty acid content), epithelial healing processes were launched early during the inflammatory flare with increased gene expression of cert 展开更多
关键词 Colon LUMINAL environment DEXTRAN sodium sulfate-induced COLITIS DYSBIOSIS EPITHELIAL repair ACUTE COLITIS
在线阅读 免费下载
Triggers of histologically suspected drug-induced colitis 预览
2
作者 Thorsten Brechmann Katharina Günther +2 位作者 Matthias Neid Wolff Schmiegel Andrea Tannapfel 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第8期967-979,共13页
BACKGROUND Drug toxicity is a common and even serious problem in the gastrointestinal tract that is thought to be caused by a broad spectrum of agents.Although withdrawal of the causative agent would cure the disease ... BACKGROUND Drug toxicity is a common and even serious problem in the gastrointestinal tract that is thought to be caused by a broad spectrum of agents.Although withdrawal of the causative agent would cure the disease knowledge is scarce and mostly derives from case reports and series.AIM To investigate potential triggers of drug-induced colitis(DiC).METHODS We conducted a retrospective,observational case control study.Patients were assigned to DiC or one of two age-and gender-matched control groups(noninflammatory controls and inflammatory colitis of another cause)based on histopathological findings.Histopathology was reassessed in a subset of patients(28 DiC with atherosclerosis,DiC without atherosclerosis and ischaemic colitis each)for validation purposes.Medical history was collected from the electronic database and patient records.Statistical analysis included chi-squared test,t-test,logistic and multivariate regression models.RESULTS Drug-induced colitis was detected in 211 endoscopically sampled biopsy specimens of the colon mucosa(7%of all screened colonoscopic biopsy samples);a total of 633 patients were included equally matched throughout the three groups(291 males,mean age:62.1±16.1 years).In the univariate analysis,DiC was associated with diuretics,dihydropyridines,glycosides,ASS,platelet aggregation inhibitors,nonsteroidal anti-inflammatory drugs(NSAIDs),statins and fibrates,and with atherosclerosis,particularly coronary heart disease,and hyperlipoproteinaemia.Echocardiographic parameters did not show substantial differences.In the multivariate analysis only fibrates[odds ratio(OR)=9.1],NSAIDs(OR=6.7)and atherosclerosis(OR=2.1)proved to be associated with DiC.Both DiC reassessment groups presented milder inflammation than ischaemic colitis.The DiC patients with atherosclerosis exhibited histological features from both DiC without atherosclerosis and ischaemic colitis.CONCLUSION Several drugs indicated for the treatment of cardiovascular and related diseases are associated with DiC.Atherosclerosis and 展开更多
关键词 DRUG toxicity DRUG-INDUCED COLITIS ISCHAEMIC COLITIS Drug-associated gastrointestinal disease Atherosclerosis Colonic ISCHAEMIA Nonsteroidal antiinflammatory drugs FIBRATES
在线阅读 免费下载
皮蛋蛋白水提物在肠道系统中的抗炎作用研究 预览
3
作者 赵燕 徐明生 +5 位作者 姚瑶 李建科 王淑珍 汪雄 王小强 涂勇刚 《中国食品学报》 EI CAS CSCD 北大核心 2019年第6期36-45,共10页
皮蛋是我国独创的一种传统大宗蛋制品,也是一种良好的“清热去火”食品。以“去大肠火”为导向,运用TNF-α诱导的Caco-2细胞炎症反应模型和DSS诱导的小鼠结肠炎模型考察皮蛋蛋白水提物的体内、外抗肠炎活性。结果表明:皮蛋蛋白水提物(5... 皮蛋是我国独创的一种传统大宗蛋制品,也是一种良好的“清热去火”食品。以“去大肠火”为导向,运用TNF-α诱导的Caco-2细胞炎症反应模型和DSS诱导的小鼠结肠炎模型考察皮蛋蛋白水提物的体内、外抗肠炎活性。结果表明:皮蛋蛋白水提物(5和10mg/mL)显著抑制Caco-2细胞IL-8的分泌,下调促炎细胞因子IL-8、TNF-α、IL-6和IL-1β基因的表达,并上调抗炎因子IL-10基因的表达。皮蛋蛋白水提物(150或300mg/kgBW)可以有效缓解DSS诱导的结肠炎小鼠体重减轻和炎症临床症状,降低促炎细胞因子TNF-α和IL-6的分泌,并下调结肠中促炎细胞因子TNF-α、IL-6、IL-1β、IFN-γ、MCP-1和IL-17A基因的表达。体内、外试验结果说明皮蛋蛋白水提物具有抗肠炎活性,可有效保护肠道健康。 展开更多
关键词 皮蛋 水提物 抗炎 CACO-2细胞 结肠炎
在线阅读 下载PDF
茵蒲灌肠液联合美沙拉秦肠溶片治疗轻中度溃疡性结肠炎的临床效果 预览
4
作者 刘将 肖尧生 +4 位作者 曾梅芳 温春虹 张华玉 张鸣青 张帅 《解放军医药杂志》 CAS 2019年第6期91-94,共4页
目的观察茵蒲灌肠液联合美沙拉秦肠溶片治疗轻中度溃疡性结肠炎(UC)的临床效果。方法选取2016年1月-2017年12月轻中度UC患者60例,按治疗方法不同分为对照组和观察组,每组30例。对照组给予美沙拉秦肠溶片,观察组在对照组基础上给予茵蒲... 目的观察茵蒲灌肠液联合美沙拉秦肠溶片治疗轻中度溃疡性结肠炎(UC)的临床效果。方法选取2016年1月-2017年12月轻中度UC患者60例,按治疗方法不同分为对照组和观察组,每组30例。对照组给予美沙拉秦肠溶片,观察组在对照组基础上给予茵蒲灌肠液保留灌肠。比较2组治疗后的临床症状、结肠镜下黏膜改变、不良反应情况以及总体疗效。结果治疗后,观察组的临床症状缓解率、结肠镜下黏膜修复有效率以及总体疗效有效率高于对照组(P<0.05)。2组的药物不良反应发生率比较差异无统计学意义(P>0.05)。结论茵蒲灌肠液联合美沙拉秦肠溶片对轻中度UC的临床症状缓解、结肠镜下黏膜修复程度均具有较好的效果,且不增加不良反应的发生率。 展开更多
关键词 结肠炎 溃疡性 茵蒲灌肠液 美沙拉秦 治疗效果
在线阅读 免费下载
Analysis on the therapeutic effects of integrated traditional Chinese and Western medicine on ulcerative colitis and its effects on coagulation function and inflammatory factors
5
作者 Qiong Wang Han-Ting Zhu +1 位作者 Guo-Rong Wu Wen Chen 《海南医科大学学报(英文版)》 2019年第1期5-9,共5页
Objective:To analyze the therapeutic effects of Integrated Traditional Chinese and Western Medicine on ulcerative colitis (UC) and its effects on coagulation function and inflammatory factors.Methods: 220 cases of pat... Objective:To analyze the therapeutic effects of Integrated Traditional Chinese and Western Medicine on ulcerative colitis (UC) and its effects on coagulation function and inflammatory factors.Methods: 220 cases of patients with UC were selected as the research subjects and randomly divided into the observation group and the control group, 110 cases were included into each group. The patients in the control group were treated with single western medicine, while the patients in the observation group were treated with enema treatment of traditional Chinese Medicine. The clinical therapeutic effects of the two groups were evaluated and compared. The blood coagulation indexes of the activated partial thromboplastin time (APTT), prothrombin time (PT), the plasma fibrinogen (Fib), the D dimer (D-D) of two groups patients who in the treatment were detected and compared. The serum tumor necrosis factor (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8) levels of the patients in the two groups were measured and compared.Results: The clinical efficiencies of the observation group and the control group were 97% and 75%, the clinical efficiencies and the therapeutic effects of the observation group were better than those in the control group, there were statistically significant differences between the two groups. The plasma Fib levels and the plasma D-D levels after the treatment of the patients in the two groups were significantly lower than those before the treatment, the difference was statistically significant. The plasma Fib levels and the plasma D-D levels after the treatment of the patients in the observation group were lower than those in the control group, the difference was statistically significant. The serum inflammatory factors levels after the treatment of the patients in the two groups were significantly lower than those before the treatment, the difference was statistically significant. The serum inflammatory factors levels after the treatment of the patients in the observation group were lower than those i 展开更多
关键词 INTEGRATED Chinese and Western Medicine ULCERATIVE COLITIS Experimental therapy COAGULATION and FIBRINOLYSIS Inflammatory factor
TRAIL基因敲除对葡聚糖硫酸钠诱导的实验性结肠炎小鼠调节性T细胞的影响
6
作者 应时杰 曹曙光 +2 位作者 夏宣平 林芊如 蒋益 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2019年第6期440-447,共8页
目的在葡聚糖硫酸钠(DSS)诱导的实验性结肠炎小鼠中探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因敲除(TRAIL-/-)对结肠炎症反应及调节性T细胞(Treg)的影响。方法选取C57BL/6品系的TRAIL-/-小鼠和野生型(WT)小鼠,随机分为WT对照组、WT... 目的在葡聚糖硫酸钠(DSS)诱导的实验性结肠炎小鼠中探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因敲除(TRAIL-/-)对结肠炎症反应及调节性T细胞(Treg)的影响。方法选取C57BL/6品系的TRAIL-/-小鼠和野生型(WT)小鼠,随机分为WT对照组、WT结肠炎组,TRAIL-/-对照组和TRAIL-/-结肠炎组,每组各10只。口服3.5%的DSS诱导实验性结肠炎模型并评估结肠炎症程度。收集外周血单个核细胞(PBMCs)和肠系膜淋巴结(MLNs),经流式细胞术检测Treg细胞占CD4+T细胞的比例,采用实时荧光定量PCR法检测Treg细胞相关转录因子(Foxp3)和细胞因子(IL-10)的mRNA表达水平,分别采用蛋白质印迹法和酶联免疫吸附试验检测Foxp3和IL-10蛋白表达水平。结果与WT对照组相比,WT结肠炎小鼠PBMCs中Treg细胞比例显著降低[(1.85±0.38)%比(3.12±0.69)%,P<0.05],而MLNs中Treg细胞比例显著增高[(11.79±1.18)%比(6.24±1.04)%,P<0.05]。与WT结肠炎小鼠相比,TRAIL-/-结肠炎小鼠结肠炎症更为严重,PBMCs中Treg细胞比例显著增高[(3.15±0.64)%比(1.85±0.38)%,P<0.05],而MLNs中Treg细胞比例显著降低[(9.80±0.50)%比(11.79±1.18)%,P<0.05]。WT结肠炎小鼠PBMCs中Foxp3、IL-10的mRNA和蛋白表达水平均显著低于WT对照组小鼠[Foxp3 mRNA:0.48±0.21比1.06±0.31,IL-10 mRNA:0.23±0.07比1.22±0.38;Foxp3蛋白:0.68±0.12比1,IL-10蛋白:(4.91 ±0.72)pg/ml比(21.86±2.40)pg/ml,P均<0.05],而MLNs中Foxp3、IL-10的mRNA和蛋白表达水平均显著高于WT对照组小鼠[Foxp3 mRNA:3.71±0.49比1.03±0.15,IL-10 mRNA:11.98±6.10比1.01±0.31;Foxp3蛋白:1.60±0.03比1,IL-10蛋白:(1 260.00±18.02)pg/ml比(1 184.00 ± 38.62)pg/ml,P均<0.05]。与WT结肠炎小鼠相比,TRAIL-/-结肠炎小鼠PBMCs中Foxp3、IL-10的mRNA及蛋白表达水平显著增高[Foxp3 mRNA:1.80±0.49比0.48±0.21,IL-10 mRNA:1.67±0.99比0.23±0.07;Foxp3蛋白:1.10±0.01比0.68±0.12,IL-10蛋白:(31.33±25.02)pg/ml比(4.58±3.73)pg/ml,P均<0.05],而MLNs中Foxp3、IL-10的mRNA及蛋白表达� 展开更多
关键词 肿瘤坏死因子相关凋亡诱导配体 调节性T细胞 葡聚糖硫酸钠 结肠炎
溃疡性结肠炎患者血清白细胞介素-lβ和白细胞介素-13水平变化
7
作者 宋丹 《中国基层医药》 CAS 2019年第6期684-686,共3页
目的探讨溃疡性结肠炎患者炎性因子白细胞介素-lβ(IL-lβ)和白细胞介素-13(IL-13)水平变化及临床意义.方法选取聊城市第四人民医院2016年1-12月收治的溃疡性结肠炎患者120例作为观察组,选择同期120例健康体检者作为对照组.采用ELLSA方... 目的探讨溃疡性结肠炎患者炎性因子白细胞介素-lβ(IL-lβ)和白细胞介素-13(IL-13)水平变化及临床意义.方法选取聊城市第四人民医院2016年1-12月收治的溃疡性结肠炎患者120例作为观察组,选择同期120例健康体检者作为对照组.采用ELLSA方法检测两组血清IL-lβ和IL-13水平.结果观察组的血清IL-lβ水平为(192.3±23.6)ng/L,明显高于对照组血清IL-lβ水平(169.8±20.3)ng/L(t=8.234,P=0.025);观察组的血清IL-13水平为(869.5±56.8)ng/L,明显低于对照组血清IL-13水平(893.2±52.1)ng/L(t=5.957,P=0.036).观察组中重度患者血清IL-lβ水平为(159.8±21.6)ng/L,均高于轻度、中度患者,差异有统计学意义(F=16.931,P=0.018);观察组中重度患者血清IL-13水平为(1132.5±142.3)ng/L,均低于轻度、中度患者,差异有统计学意义(F=15.049,P=0.022).Pearson相关分析显示,观察组血清IL-lβ和IL-13水平呈负相关(r=-0.547,P=0.036).结论血清IL-lβ和IL-13水平且其在不同严重程度的消化性溃疡中也具有不同程度的表达,可作为消化性溃疡患者的检测指标,为临床诊断消化性溃疡提供依据. 展开更多
关键词 结肠炎 溃疡性 白细胞介素1Β 白细胞介素13
艾灸预处理天枢穴对溃疡性结肠炎大鼠结肠TLR4/TRIF信号通路调节作用的研究 预览
8
作者 杨玲 黄艳 +8 位作者 黄任佳 马晓芃 马喆 刘雅楠 郑寒丹 朱毅 刘慧荣 王照钦 吴璐一 《上海针灸杂志》 2019年第8期819-826,共8页
目的观察艾灸预处理天枢穴对溃疡性结肠炎(ulcerative colitis,UC)大鼠结肠TLR4/TRIF信号通路的影响,探讨艾灸预处理防治UC的作用机制.方法将28只SD雄性大鼠随机分为正常组、模型组、隔药灸预处理组和温和灸预处理组.采用4%葡聚糖硫酸... 目的观察艾灸预处理天枢穴对溃疡性结肠炎(ulcerative colitis,UC)大鼠结肠TLR4/TRIF信号通路的影响,探讨艾灸预处理防治UC的作用机制.方法将28只SD雄性大鼠随机分为正常组、模型组、隔药灸预处理组和温和灸预处理组.采用4%葡聚糖硫酸钠水溶液连续饮用7 d制备UC模型.隔药灸预处理组、温和灸预处理组先干预7 d后,模型组、隔药灸预处理组、温和灸预处理组再制备UC模型.采用HE染色法,观察大鼠结肠组织病理学变化;应用免疫组化、免疫印记技术检测大鼠结肠TLR4、TRIF、TRAF6、IRF3、IKK的蛋白表达.结果模型组大鼠结肠黏膜结构损伤较正常组明显加重;隔药灸预处理组和温和灸预处理组,结肠黏膜结构损伤较模型组明显改善.与正常组比较,模型组结肠TLR4、TRIF、TRAF6、IRF3、IKK蛋白表达均显著升高(P<0.05);与模型组比较,隔药灸预处理组、温和灸预处理组结肠TLR4、TRIF、TRAF6、IRF3蛋白表达均显著降低(P<0.05),隔药灸预处理组结肠IKK蛋白表达显著降低(P<0.05).结论艾灸预处理天枢穴能下调UC大鼠结肠TLR4、TRIF、TRAF6、IRF3蛋白表达;下调TLR4/TRIF信号通路相关分子的蛋白表达可能是艾灸预处理防治UC的作用机制之一. 展开更多
关键词 灸法 药饼灸疗法 艾条灸 天枢 艾灸预处理 结肠炎 溃疡性 TLR4/TRIF通路 作用机制
在线阅读 免费下载
溃疡性结肠炎肠外表现的危险因素分析 预览
9
作者 木克热木·依明尼亚孜 高峰 《胃肠病学》 2019年第5期293-297,共5页
背景:溃疡性结肠炎(UC)伴有多种肠外表现(EIM),EIM对UC的诊治有重要作用。目的:探讨UC患者EIM的发生情况及其危险因素。方法:回顾性分析2013年1月-2018年12月新疆维吾尔自治区人民医院确诊为UC的临床资料,采用单因素、多因素分析探讨UC... 背景:溃疡性结肠炎(UC)伴有多种肠外表现(EIM),EIM对UC的诊治有重要作用。目的:探讨UC患者EIM的发生情况及其危险因素。方法:回顾性分析2013年1月-2018年12月新疆维吾尔自治区人民医院确诊为UC的临床资料,采用单因素、多因素分析探讨UC相关EIM的危险因素。结果:共纳入260例UC患者,其中120例伴有EIM,发生率为46.2%,主要包括关节病变、皮肤病变、眼部病变、肝胆疾病等。单因素分析发现,维吾尔族(χ^2=4.069,P=0.044)、老年(χ^2=4.250,P=0.039)、BMI≥24 kg/m^2(χ^2=13.913,P=0.000)、肠手术史(χ^2=6.089,P=0.014)和疾病活动性(χ^2=27.556,P=0.000)均与EIM有关。多因素分析发现,维吾尔族(OR=1.802,P=0.042)、BMI≥24 kg/m^2(OR=3.163,P=0.000)、肠手术史(OR=4.089,P=0.042)和疾病活动性(OR=6.347,P=0.000)为EIM的危险因素。结论:维吾尔族、BMI≥24 kg/m^2、肠手术史和疾病活动性是UC患者发生EIM的高危因素。 展开更多
关键词 结肠炎 溃疡性 肠外表现 危险因素 回顾性研究
在线阅读 下载PDF
中重度溃疡性结肠炎患者肠内营养支持治疗的回顾性分析 预览
10
作者 王凤纤 缪应雷 +2 位作者 张雪芹 唐源 李红纳 《胃肠病学》 2019年第4期235-238,共4页
背景:肠内营养(EN)能促进肠黏膜愈合,对克罗恩病(CD)患者具有一定的治疗价值,但在溃疡性结肠炎(UC)中应用和疗效的研究较少。目的:探讨EN对具有营养风险的中重度活动期UC患者的疗效。方法:选取2016年1月-2018年1月曲靖市第一人民医院、... 背景:肠内营养(EN)能促进肠黏膜愈合,对克罗恩病(CD)患者具有一定的治疗价值,但在溃疡性结肠炎(UC)中应用和疗效的研究较少。目的:探讨EN对具有营养风险的中重度活动期UC患者的疗效。方法:选取2016年1月-2018年1月曲靖市第一人民医院、昆明医科大学第一附属医院90例具有营养风险的中重度UC患者,并分为EN组和无EN组。比较两组治疗前后Mayo评分、体重指数(BMI)、血红蛋白(HGB)、白蛋白(ALB)、总蛋白(TP)水平。结果:治疗前,EN组与无EN组Mayo评分、BMI、HGB、ALB和TP相比差异均无统计学意义(P>0. 05)。与治疗前相比,治疗后EN组Mayo评分显著降低(P <0. 05),ALB、TP显著升高(P <0. 05);无EN组Mayo评分、BMI均显著降低(P <0. 05)。治疗后,EN组BMI、ALB、TP均显著高于无EN组(P <0. 05)。结论:EN能有效改善UC患者的营养状况,对病情的改善起有辅助作用。 展开更多
关键词 结肠炎 溃疡性 营养风险 肠道营养 治疗
在线阅读 下载PDF
非侵入性分子标志物对溃疡性结肠炎内镜活动度的判断价值 预览
11
作者 陈金敏 高山 童旭东 《胃肠病学》 2019年第5期298-301,共4页
背景:溃疡性结肠炎(UC)是复发与缓解交替的肠道慢性炎症性疾病,及时判断疾病活动度对指导临床医师制定合理的治疗方案以及预测患者预后具有重要意义。目的:评估非侵入性分子标志物对UC患者内镜活动度的判断价值。方法:选取2016年8月-201... 背景:溃疡性结肠炎(UC)是复发与缓解交替的肠道慢性炎症性疾病,及时判断疾病活动度对指导临床医师制定合理的治疗方案以及预测患者预后具有重要意义。目的:评估非侵入性分子标志物对UC患者内镜活动度的判断价值。方法:选取2016年8月-2018年3月襄阳市中心医院收治的56例UC患者,以25例肠易激综合征(IBS)患者作为对照。采用ELISA法测定粪钙卫蛋白(FC)浓度,评估临床活动指数(CAI)评分,检测C反应蛋白(CRP)、红细胞沉降率(ESR),以Mayo评分评估内镜活动度。分析非侵入性标志物对UC内镜活动度的判断价值。结果:UC患者FC浓度明显高于IBS患者(P<0.001)。活动期UC患者FC、CAI、CRP、ESR均显著高于缓解期UC患者(P<0.001)。UC患者FC、CAI、CRP、ESR与Mayo评分均相关(r分别为0.814、0.724、0.610、0.657,P均<0.001)。FC临界值为200μg/g时,预测UC内镜活动度的敏感性和特异性分别为92.3%和94.1%。结论:与CAI、CRP、ESR相比,FC能更有效地发现UC患者的内镜下活动性炎症。 展开更多
关键词 结肠炎 溃疡性 粪钙卫蛋白 内镜活动度 C反应蛋白质 血沉
在线阅读 下载PDF
溃疡性结肠炎相关免疫分子及其临床应用的研究进展 预览
12
作者 金晨星 李芳 《中国免疫学杂志》 CAS CSCD 北大核心 2019年第4期505-508,F0004共5页
溃疡性结肠炎(UC)的发病率近年明显上升,但其病因尚未完全明确,研究发现多种免疫分子参与UC的发病并且在临床应用中具有重要作用。其中自身抗体、TNF-α、白细胞介素、中性肽链内切酶等均参与UC的发病;主要作用体现在其异常表达以及相... 溃疡性结肠炎(UC)的发病率近年明显上升,但其病因尚未完全明确,研究发现多种免疫分子参与UC的发病并且在临床应用中具有重要作用。其中自身抗体、TNF-α、白细胞介素、中性肽链内切酶等均参与UC的发病;主要作用体现在其异常表达以及相互作用使肠道免疫稳态被破坏,导致肠道炎症的发生、肠道黏膜损伤并使其持续存在;同时对其进行血清学检测有助于判断疾病严重程度及治疗效果。抗TNF-α治疗、改变肠道色氨酸代谢通路、抑制肠上皮细胞细胞因子信号传导抑制蛋白3的表达、增强IL-22/信号转导子及转录激活子3信号传导将有助于治疗UC。明确免疫分子在UC中的作用将为临床诊断、治疗等方面提供帮助。 展开更多
关键词 结肠炎 溃疡性 自身抗体 肿瘤坏死因子Α 白细胞介素类
在线阅读 下载PDF
粪菌移植体系与炎症性肠病的研究进展 预览
13
作者 王宏刚 何兴祥 +1 位作者 王寒 杨晓钟 《胃肠病学》 2019年第5期302-306,共5页
粪菌移植(FMT)作为一项新兴的治疗技术体系,主要涉及严格的供体筛选、优化的粪菌制备方法、恰当的菌群移植途径等。本文就FMT体系与炎症性肠病(IBD)的研究进展作一综述。
关键词 粪菌移植 CROHN病 结肠炎 溃疡性 安全 经内镜肠道植管术
在线阅读 下载PDF
NADPH氧化酶Nox2和Nox4在小鼠肠炎中的表达及意义 预览
14
作者 肖中岳 轩青霞 高强 《安徽医科大学学报》 CAS 北大核心 2019年第2期225-230,共6页
目的探讨烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶家族的主要成员NADPH氧化酶2 (Nox2)和Nox4在小鼠肠炎模型结肠组织中的表达及意义。方法选用6~8周龄的129S/SV雄性小鼠建立结肠炎模型,将其随机分为对照组、1. 5%葡聚糖硫酸钠(DSS)组和3.... 目的探讨烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶家族的主要成员NADPH氧化酶2 (Nox2)和Nox4在小鼠肠炎模型结肠组织中的表达及意义。方法选用6~8周龄的129S/SV雄性小鼠建立结肠炎模型,将其随机分为对照组、1. 5%葡聚糖硫酸钠(DSS)组和3. 0%DSS组,对照组自由饮水,1. 5%DSS组和3. 0%DSS组分别给予含1. 5%DSS和3. 0%DSS饮用水自由饮用6 d。通过体质量变化、疾病活动指数(DAI)评分和组织病理学评分等方法评估肠道炎症程度。使用酶标仪间接测定小鼠血清中丙二醛(MDA)的含量,以评估实验小鼠的氧化应激程度。采用实时定量PCR技术检测结肠组织中促炎因子白介素-1β(IL-1β)、白介素-6 (IL-6)和肿瘤坏死因子-α(TNF-α)以及Nox2和Nox4 mRNA表达情况;采用免疫组织化学法检测结肠组织中Nox2和Nox4蛋白表达情况。结果对照组小鼠未见肠炎表现,1. 5%DSS组小鼠呈轻度肠炎表现,3. 0%DSS组小鼠呈重度肠炎表现。杯状细胞在1. 5%DSS组体积增大、数量减少,在3. 0%DSS组进一步减少,甚至消失(均P <0. 05)。MDA在1. 5%DSS组升高,在3. 0%DSS组进一步升高(均P <0. 05)。Nox2和Nox4 mRNA和蛋白的表达量在不同炎症程度时表达不同。两者mRNA和蛋白的表达量一致,炎症组均显著高于对照组,且均随炎症程度增加表达进一步增加(均P <0. 05)。Nox2蛋白主要表达于浸润的吞噬细胞和中性粒细胞等炎细胞中;Nox4蛋白表达于中性粒细胞和淋巴细胞等炎细胞中。结论 Nox2和Nox4在小鼠肠炎的发病过程中发挥重要作用。 展开更多
关键词 NADPH氧化酶 Nox2 NOX4 炎症性肠病 结肠炎
在线阅读 下载PDF
Role of cytochrome P450 polymorphisms and functions in development of ulcerative colitis 预览
15
作者 Alaattin Sen Holger Stark 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第23期2846-2862,共17页
Cytochromes P450 s(CYPs) are terminal enzymes in CYP dependent monooxygenases, which constitute a superfamily of enzymes catalysing the metabolism of both endogenous and exogenous substances. One of their main tasks i... Cytochromes P450 s(CYPs) are terminal enzymes in CYP dependent monooxygenases, which constitute a superfamily of enzymes catalysing the metabolism of both endogenous and exogenous substances. One of their main tasks is to facilitate the excretion of these substances and eliminate their toxicities in most phase 1 reactions. Endogenous substrates of CYPs include steroids, bile acids, eicosanoids, cholesterol, vitamin D and neurotransmitters. About 80% of currently used drugs and environmental chemicals comprise exogenous substrates for CYPs. Genetic polymorphisms of CYPs may affect the enzyme functions and have been reported to be associated with various diseases and adverse drug reactions among different populations. In this review, we discuss the role of some critical CYP isoforms(CYP1 A1, CYP2 D6, CYP2 J2, CYP2 R1,CYP3 A5, CYP3 A7, CYP4 F3, CYP24 A1, CYP26 B1 and CYP27 B1) in the pathogenesis or aetiology of ulcerative colitis concerning gene polymorphisms. In addition, their significance in metabolism concerning ulcerative colitis in patients is also discussed showing a clear underestimation in genetic studies performed so far. 展开更多
关键词 CYTOCHROME P450 Polymorphism Function ULCERATIVE COLITIS AETIOLOGY
在线阅读 免费下载
日本血吸虫成虫可溶性蛋白和重组半胱氨酸蛋白酶抑制蛋白抑制小鼠结肠炎的研究
16
作者 武艺 李路 +6 位作者 徐永伟 邢瑞欣 胡静 王书书 沈继龙 徐元宏 陈熙 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 2019年第2期127-136,共10页
目的探讨日本血吸虫可溶性成虫蛋白(SjSWP)和重组半胱氨酸蛋白酶抑制蛋白(rSjcystatin)对模拟炎症性肠病的小鼠结肠炎的作用。方法24只BALB/c雌鼠分为健康对照组、磷酸盐缓冲液组(PBS组)、rSjcystatin治疗组(rSjcystatin组)、SjSWP治疗... 目的探讨日本血吸虫可溶性成虫蛋白(SjSWP)和重组半胱氨酸蛋白酶抑制蛋白(rSjcystatin)对模拟炎症性肠病的小鼠结肠炎的作用。方法24只BALB/c雌鼠分为健康对照组、磷酸盐缓冲液组(PBS组)、rSjcystatin治疗组(rSjcystatin组)、SjSWP治疗组(SjSWP组)。PBS、rSjcystatin、SjSWP组用0.1ml5%2,4,6-三硝基苯磺酸(TNBS)灌肠诱导结肠炎,灌肠后6、24h分别腹腔注射100μlPBS、50μgrSjcystatin、50μgSjSWP;健康对照组在相应时间点用等量的PBS灌肠及腹腔注射。TNBS灌肠72h后无痛处死各组小鼠,检测宏观评分、结肠长度、髓过氧化物酶活性和显微镜下微观评分来评估炎症参数和病理改变。流式细胞仪检测小鼠脾脏中Th1和Th2及T调节细胞(Tregs)的比例。ELISA检测小鼠结肠组织匀浆上清中γ干扰素(IFN-γ)、白细胞介素-4(IL-4)、IL-13、IL-10等炎性细胞因子的水平。多组计量资料分析采用单因素方差分析,多组均数间的两两比较用Student-Newman-Keuls检验。结果TNBS诱导的结肠炎小鼠表现出明显的炎症和病理学特征,表现为体质量下降,便血、腹泻、结肠缩短、溃疡,以及炎性细胞因子IFN-γ、炎性细胞浸润和髓过氧化物酶活性的增加。与PBS组相比,rSjcystatin和SjSWP的干预治疗可以显著改善TNBS诱导的结肠炎症,表现为宏观评分(改善结肠糜烂、黏连、溃疡和缩短)和微观评分(减少组织损伤和炎症)的降低。健康对照组、PBS组、rSjcystatin组和SjSWP组小鼠结肠的宏观评分分别为0、9.8±0.8、4.3±1.0和4.5±1.4,微观评分分别为0、6.0±0.4、2.2±0.5和2.8±0.6,rSjcystatin组、SjSWP组与PBS组相比差异均有统计学意义(F=57.8、34.1,P<0.01),rSjcystatin组与SjSWP组间差异均无统计学意义(P>0.05)。健康对照组、PBS组、rSjcystatin组和SjSWP组髓过氧化物酶分别为0.3±0.0、1.4±0.1、0.6±0.1和0.6±0.1(F=32.5,P<0.01);脾脏Tregs比例分别为(4.4±0.7)%、(8.2±3.1)%、(18.4±2.1)%和(13.4±1.9)% 展开更多
关键词 日本血吸虫 可溶性成虫蛋白 半胱氨酸蛋白酶抑制蛋白 结肠炎 免疫调节
Phantom of the inflammasome in the gut:Cytomegalovirus 预览
17
作者 Ferhat Arslan Haluk Vahaboglu 《世界荟萃分析杂志》 2019年第7期346-349,共4页
Cytomegalovirus(CMV)is frequently detected in inflammatory bowel tissue,especially in corticosteroid-refractory patients,and it has been blamed for adverse outcomes.However,the first acquisition of CMV does not involv... Cytomegalovirus(CMV)is frequently detected in inflammatory bowel tissue,especially in corticosteroid-refractory patients,and it has been blamed for adverse outcomes.However,the first acquisition of CMV does not involve the colon.In particular in the colonic mucosa,which evolved due to the gut microbial relationship,CMV promotes inflammation via recruited monocytes and not through replication in resident macrophages.Whether CMV is the last straw in the process of mucosal inflammation,a doomed agent,or an innocent bystander is a difficult question that remains elusive.With this work,we will try to review the relationship between intestinal mucosa and CMV in the framework of basic virological principles. 展开更多
关键词 CYTOMEGALOVIRUS ULCERATIVE COLITIS Gancyclovir Inflammatory bowel disease
在线阅读 免费下载
Pediatric recurrent Clostridium difficile infections in immunocompetent children:Lessons learned from case reports of the first twelve consecutive patients 预览
18
作者 Angela Chu Sonia Michail 《世界荟萃分析杂志》 2019年第7期350-357,共8页
BACKGROUND Recurrent Clostridium difficile infection(CDI)in children can be difficult to manage and may represent an unidentified underlying pathology.Recurrence can be frequently encountered in immunodeficiency disor... BACKGROUND Recurrent Clostridium difficile infection(CDI)in children can be difficult to manage and may represent an unidentified underlying pathology.Recurrence can be frequently encountered in immunodeficiency disorders and inflammatory bowel disease(IBD).AIM To report cases of a select population of children with recurrent CDI who are immunocompetent and do not have an identified IBD and examine the potential for any underlying risk factors,disease course and disease outcome.METHODS Review of charts for children aged 1-21 years with recurrent CDI referred to see pediatric gastroenterology service was performed.All subjects with known immunosuppression or IBD were excluded.Subjects were followed for at least 24 mo.RESULTS Twelve children seen consecutively were identified.All patients were treated with antibiotic courses for CDI prior to their referral.Five out of 12 patients had an underlying pathology that was not previously identified,including eosinophilic colitis and IBD.CDI symptoms resolved after treatment of underlying colitis without the need to target therapy for CDI.There were 9 patients that failed antibiotic treatment of CDI and required fecal microbiome transplant,which was safe and highly effective in preventing recurrence(100%efficacy).The gut microbial changes after fecal transplant were characterized by a remarkable and durable increase in diversity and in abundance of Bacteroides.CONCLUSION Pediatric patients with frequent recurrence of CDI may have an unidentified underlying gastrointestinal pathology that may warrant further investigation by a specialist who can identify these diseases and help optimize management.Many of these children may benefit from fecal microbial transplant which appears to be a safe,highly effective therapy that results in long term changes in the gut microbiome. 展开更多
关键词 RECURRENT Clostridium DIFFICILE infection EOSINOPHILIC colitis Inflammatory bowel disease FECAL MICROBIOME transplant
在线阅读 免费下载
美沙拉嗪联合双歧杆菌三联活菌治疗溃疡性结肠炎的临床疗效及其安全性
19
作者 国春红 《临床合理用药杂志》 2019年第12期23-24,共2页
目的探讨美沙拉嗪联合双歧杆菌三联活菌治疗溃疡性结肠炎的临床疗效及其安全性。方法选取新泰市人民医院2014年6月-2018年6月收治的溃疡性结肠炎患者120例,随机分为对照组和治疗组,各60例。对照组患者采用美沙拉嗪治疗,治疗组患者在对... 目的探讨美沙拉嗪联合双歧杆菌三联活菌治疗溃疡性结肠炎的临床疗效及其安全性。方法选取新泰市人民医院2014年6月-2018年6月收治的溃疡性结肠炎患者120例,随机分为对照组和治疗组,各60例。对照组患者采用美沙拉嗪治疗,治疗组患者在对照组基础上采用双歧杆菌三联活菌治疗,两组患者均持续治疗2个疗程。比较两组患者临床疗效及治疗前后超敏C反应蛋白(hs-CRP)、肿瘤坏死因子α(TNF-α),并观察两组患者不良反应发生情况。结果治疗组患者治疗总有效率高于对照组(P<0.05)。治疗前,两组患者hs-CRP、TNF-α水平比较,差异无统计学意义(P>0.05);治疗后,治疗组患者hs-CRP、TNF-α水平低于对照组(P<0.05)。治疗组患者不良反应发生率低于对照组(P<0.05)。结论美沙拉嗪联合双歧杆菌三联活菌治疗溃疡性结肠炎的临床疗效确切,其可有效减轻机体炎性反应,且安全性较高。 展开更多
关键词 结肠炎 溃疡性 美沙拉嗪 双歧杆菌三联活菌 治疗结果
Bone alterations in inflammatory bowel diseases 预览
20
作者 Dolores Sgambato Francesca Gimigliano +8 位作者 Cristiana De Musis Antimo Moretti Giuseppe Toro Emanuele Ferrante Agnese Miranda Domenico De Mauro Lorenzo Romano Giovanni Iolascon Marco Romano 《世界临床病例杂志》 2019年第15期1908-1925,共18页
Inflammatory bowel diseases(IBDs)are characterized by a multifactorial partially unknown etiology that involves genetic,immunological and environmental factors.Up to 50%of IBD patients experience at least one extraint... Inflammatory bowel diseases(IBDs)are characterized by a multifactorial partially unknown etiology that involves genetic,immunological and environmental factors.Up to 50%of IBD patients experience at least one extraintestinal manifestation;among them is the involvement of bone density which is referred to as metabolic bone disease(MBD),including osteopenia and osteoporosis.Bone alterations in IBDs population appear to have a multifactorial etiology:Decreased physical activity,inflammation-related bone resorption,multiple intestinal resections,dietary malabsorption of minerals and vitamin D deficiency,genetic factors,gut-bone immune signaling interaction,steroid treatment,microbiota and pathogenic micro-organisms interaction,and dietary malabsorption of minerals,that,all together or individually,may contribute to the alteration of bone mineral density.This review aims to summarize the prevalence and pathophysiology of metabolic bone alterations in IBD subjects outlining the main risk factors of bone fragility.We also want to underline the role of the screening and prophylaxis of bone alterations in Crohn’s disease and ulcerative colitis patients and the importance of treating appropriately MBD. 展开更多
关键词 Inflammatory BOWEL diseases BONE alterations BONE mineral density OSTEOPOROSIS OSTEOPENIA ULCERATIVE COLITIS Crohn’s disease
在线阅读 免费下载
上一页 1 2 250 下一页 到第
使用帮助 返回顶部 意见反馈