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Predicting differentiation potential of human pluripotent stem cells:Possibilities and challenges 预览
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作者 Li-Ping Liu Yun-Wen Zheng 《世界干细胞杂志:英文版(电子版)》 2019年第7期375-382,共8页
The capability of human pluripotent stem cell(hPSC)lines to propagate indefinitely and differentiate into derivatives of three embryonic germ layers makes these cells be powerful tools for basic scientific research an... The capability of human pluripotent stem cell(hPSC)lines to propagate indefinitely and differentiate into derivatives of three embryonic germ layers makes these cells be powerful tools for basic scientific research and promising agents for translational medicine.However,variations in differentiation tendency and efficiency as well as pluripotency maintenance necessitate the selection of hPSC lines for the intended applications to save time and cost.To screen the qualified cell lines and exclude problematic cell lines,their pluripotency must be confirmed initially by traditional methods such as teratoma formation or by highthroughput gene expression profiling assay.Additionally,their differentiation potential,particularly the lineage-specific differentiation propensities of hPSC lines,should be predicted in an early stage.As a complement to the teratoma assay,RNA sequencing data provide a quantitative estimate of the differentiation ability of hPSCs in vivo.Moreover,multiple scorecards have been developed based on selected gene sets for predicting the differentiation potential into three germ layers or the desired cell type many days before terminal differentiation.For clinical application of hPSCs,the malignant potential of the cells must also be evaluated.A combination of histologic examination of teratoma with quantitation of gene expression data derived from teratoma tissue provides safety-related predictive information by detecting immature teratomas,malignancy marker expression,and other parameters.Although various prediction methods are available,distinct limitations remain such as the discordance of results between different assays and requirement of a long time and high labor and cost,restricting their wide applications in routine studies.Therefore,simpler and more rapid detection assays with high specificity and sensitivity that can be used to monitor the status of hPSCs at any time and fewer targeted markers that are more specific for a given desired cell type are urgently needed. 展开更多
关键词 Human PLURIPOTENT STEM CELLS Induced PLURIPOTENT STEM CELLS Embryonic STEM CELLS DIFFERENTIATION POTENTIAL Prediction Pluripotency Malignant POTENTIAL EMBRYOID bodies Lineage-specific DIFFERENTIATION Teratoma
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成人鼻腔呼吸黏膜内间充质干细胞的分化特征及其多向诱导分化潜能研究 预览
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作者 郭照萌 侯铁奇 《中国耳鼻咽喉颅底外科杂志》 CAS 2019年第4期412-418,共7页
目的研究成人鼻腔呼吸黏膜内间充质干细胞(mesenchymal stem cells,MSCs)的分化特征及其多向诱导分化潜能。方法取材及细胞外培养成人鼻腔呼吸黏膜,鼻黏膜组织切片及MSCs免疫荧光染色(体外培养),鼻黏膜MSCs向成骨细胞诱导分化及向神经... 目的研究成人鼻腔呼吸黏膜内间充质干细胞(mesenchymal stem cells,MSCs)的分化特征及其多向诱导分化潜能。方法取材及细胞外培养成人鼻腔呼吸黏膜,鼻黏膜组织切片及MSCs免疫荧光染色(体外培养),鼻黏膜MSCs向成骨细胞诱导分化及向神经细胞诱导分化。结果Vimentin抗体及Nestin抗体免疫荧光染色成人下鼻甲黏膜冷冻切片显示,双标记的阳性细胞为卵圆形或圆形,在鼻黏膜固有层中广泛分布。免疫荧光染色结果显示干细胞标志蛋白Sall4、CD133、Nestin、Vimentin受传代培养的MSCs表达。钙结节的茜素红染色结果:向成骨细胞诱导培养鼻黏膜MSCs 2周后,茜素红染色显示有大量红色钙结节存在于细胞表面,没有显著钙结节存在于未经诱导的MSCs表面,只有少量茜素红着色;碱性磷酸酶染色结果:碱性磷酸酶染色在向成骨细胞诱导培养鼻黏膜MSCs2周后呈紫蓝色,未经诱导的MSCs具有极浅的染色。用神经诱导培养液进行2周的诱导后,细胞向神经细胞样显著分化,可见大部分细胞呈BM88与NF-200免疫染色阳性,细胞将多根细长突起发出并相互连接,对照组细胞具有极弱的荧光染色,为长梭形。结论成人鼻腔呼吸黏膜内MSCs广泛存在,具有多向诱导分化潜能,在对骨与软组织损伤进行自体移植修复的过程中可以作为种子细胞得到有效应用,期待临床进一步验证。 展开更多
关键词 鼻腔呼吸黏膜 成人 内间充质干细胞 分化特征 多向诱导分化潜能
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诱导多能干细胞向雄性生殖细胞分化诱导物的研究进展
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作者 李欣 赵中利 +4 位作者 罗晓彤 曹阳 张立春 于永生 金海国 《中国生物工程杂志》 CAS CSCD 北大核心 2019年第4期94-100,共7页
诱导多能干细胞(induced pluripotent stem cells,iPSCs)是利用细胞重编程技术人工获得的与胚胎干细胞(embryonic stem cells,ESCs)功能类似的细胞,能分化成包括三胚层在内的所有细胞类型,并且规避了ESCs的伦理学争议和移植后的免疫排... 诱导多能干细胞(induced pluripotent stem cells,iPSCs)是利用细胞重编程技术人工获得的与胚胎干细胞(embryonic stem cells,ESCs)功能类似的细胞,能分化成包括三胚层在内的所有细胞类型,并且规避了ESCs的伦理学争议和移植后的免疫排斥问题,具有十分广阔的应用前景。对iPSCs体外诱导为生殖细胞所用的诱导物及其诱导效果进行了综述,生殖细胞发育机制的研究有望促进未来生殖和发育技术的进步。 展开更多
关键词 诱导多能干细胞 雄性生殖细胞 分化 诱导物
考虑要素投入与市场地位的物流联盟演化研究 预览
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作者 陈信同 李帮义 +2 位作者 王哲 马晓平 周扬 《运筹与管理》 CSSCI CSCD 北大核心 2019年第7期64-71,共8页
针对物流联盟成员间的差异化,研究物流联盟的演化与稳定问题。以企业的要素投入与市场地位为依据,将联盟成员划分为三类,并构建三者之间的演化博弈模型,分析了影响博弈主体策略选择的影响因素,通过模型分析确立三方策略趋于稳定的条件... 针对物流联盟成员间的差异化,研究物流联盟的演化与稳定问题。以企业的要素投入与市场地位为依据,将联盟成员划分为三类,并构建三者之间的演化博弈模型,分析了影响博弈主体策略选择的影响因素,通过模型分析确立三方策略趋于稳定的条件。结论表明,联盟的惩罚力度、成员间的交易费用以及投机获利对联盟成员的策略选择以及物流联盟的稳定性有着重要影响。联盟成员间的激励、优惠政策比单方面的联盟惩罚更能促进联盟成员的积极合作。 展开更多
关键词 物流联盟 差异化 演化博弈 三方博弈
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Enhanced hepatic differentiation in the subpopulation of human amniotic stem cells under 3D multicellular microenvironment 预览
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作者 Kinji Furuya Yun-Wen Zheng +11 位作者 Daisuke Sako Kenichi Iwasaki Dong-Xu Zheng Jian-Yun Ge Li-Ping Liu Tomoaki Furuta Kazunori Akimoto Hiroya Yagi Hiromi Hamada Hiroko Isoda Tatsuya Oda Nobuhiro Ohkohchi 《世界干细胞杂志:英文版(电子版)》 2019年第9期705-721,共17页
BACKGROUND To solve the problem of liver transplantation donor insufficiency,an alternative cell transplantation therapy was investigated.We focused on amniotic epithelial cells(AECs)as a cell source because,unlike in... BACKGROUND To solve the problem of liver transplantation donor insufficiency,an alternative cell transplantation therapy was investigated.We focused on amniotic epithelial cells(AECs)as a cell source because,unlike induced pluripotent stem cells,they are cost-effective and non-tumorigenic.The utilization of AECs in regenerative medicine,however,is in its infancy.A general profile for AECs has not been comprehensively analyzed.Moreover,no hepatic differentiation protocol for AECs has yet been established.To this end,we independently compiled human AEC libraries,purified amniotic stem cells(ASCs),and co-cultured them with mesenchymal stem cells(MSCs)and human umbilical vein endothelial cell(HUVECs)in a 3D system which induces functional hepatic organoids.AIM To characterize AECs and generate functional hepatic organoids from ASCs and other somatic stem cells METHODS AECs,MSCs,and HUVECs were isolated from the placentae and umbilical cords of cesarean section patients.Amnion and primary AEC stemness characteristics and heterogeneity were analyzed by immunocytochemistry,Alkaline phosphatase(AP)staining,and flow cytometry.An adherent AEC subpopulation was selected and evaluated for ASC purification quality by a colony formation assay.AEC transcriptomes were compared with those for other hepatocytes cell sources by bioinformatics.The 2D and 3D culture were compared by relative gene expression using several differentiation protocols.ASCs,MSCs,and HUVECs were combined in a 3D co-culture system to generate hepatic organoids whose structure was compared with a 3D AEC sphere and whose function was elucidated by immunofluorescence imaging,periodic acid Schiff,and an indocyanine green(ICG)test.RESULTS AECs have certain stemness markers such as EPCAM,SSEA4,and E-cadherin.One AEC subpopulation was also either positive for AP staining or expressed the TRA-1-60 and TRA-1-81 stemness markers.Moreover,it could form colonies and its frequency was enhanced ten-fold in the adherent subpopulation after selective primary passage.Bioinfo 展开更多
关键词 3D MICROPATTERN Amniotic epithelial CELLS Amniotic STEM CELLS Hepatic differentiation Heterogeneity HUMAN PLACENTAL tissue HUMAN umbilical vein endothelial CELLS Mesenchymal STEM CELLS Multicellular microenvironment Organoid
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财政补贴、研发投入与企业创新绩效——基于创业板上市公司的经验数据 预览
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作者 马克和 张婷婷 《河北经贸大学学报》 CSSCI 北大核心 2019年第6期34-40,共7页
基于2012—2016年创业板上市公司的数据,实证分析财政补贴、企业研发投入与企业创新绩效之间的关系。研究发现:从总体上来看财政补贴与研发投入对企业创新绩效起促进作用,财政补贴对企业自主研发存在挤入效应;从局部来看财政补贴对企业... 基于2012—2016年创业板上市公司的数据,实证分析财政补贴、企业研发投入与企业创新绩效之间的关系。研究发现:从总体上来看财政补贴与研发投入对企业创新绩效起促进作用,财政补贴对企业自主研发存在挤入效应;从局部来看财政补贴对企业创新绩效的调节效果在不同地区、行业和企业间有所差异。为此,政府要制定更加灵活有针对性的补贴政策并强化监管,做到加力提效,努力提高企业创新效率,助力经济高质量发展。 展开更多
关键词 财政补贴 研发投入 创新绩效 差异化
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TDO2对梅花鹿鹿茸软骨细胞分化的影响
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作者 丁军莉 马莉 +3 位作者 柳舒 岳占碰 郭斌 杨占清 《中国兽医学报》 CAS CSCD 北大核心 2019年第2期292-295,共4页
为了研究色氨酸2,3-双加氧酶(TDO2)在梅花鹿茸角再生中的作用,本试验通过原位杂交方法检测TDO2mRNA在梅花鹿茸角中的表达。在体外分离培养的鹿茸软骨细胞中添加TDO2抑制剂680C91,诱导24h后,应用Real-time PCR方法检测肥大软骨细胞分化... 为了研究色氨酸2,3-双加氧酶(TDO2)在梅花鹿茸角再生中的作用,本试验通过原位杂交方法检测TDO2mRNA在梅花鹿茸角中的表达。在体外分离培养的鹿茸软骨细胞中添加TDO2抑制剂680C91,诱导24h后,应用Real-time PCR方法检测肥大软骨细胞分化标志分子Ⅹ型胶原(COLⅩ)及RUNT相关转录因子3(RUNX3)mRNA表达的变化,进一步研究TDO2对鹿茸软骨细胞分化的调节机理。结果发现,TDO2在鹿茸真皮层、间充质层及软骨层中均有表达。680C91诱导24h后,鹿茸软骨细胞中COLⅩ的表达量显著降低,同时RUNX3的表达水平也显著降低。以上结果表明,TDO2可能通过RUNX3来调节梅花鹿鹿茸软骨细胞的分化。 展开更多
关键词 色氨酸2 3-双加氧酶 软骨细胞 分化 鹿茸
The genomic and microenvironmental factors affecting neuroendocrine differentiation in prostate cancer induced by androgen deprivation therapy 预览
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作者 Ya-Nan Man Yan-Fang Chen 《精准医学研究》 2019年第1期9-16,共8页
Although advanced prostate cancer(PCa)can be initially controlled by androgen deprivation therapy(ADT),recurrence normally occurs due to the appearance of castration-resistant prostate cancer(CRPC).Neuroendocrine diff... Although advanced prostate cancer(PCa)can be initially controlled by androgen deprivation therapy(ADT),recurrence normally occurs due to the appearance of castration-resistant prostate cancer(CRPC).Neuroendocrine differentiation(NED)in PCa can be partly explained the androgen resistance and progression to CRPC.NED normally associates with more aggressive clinical behaviour and poor outcomes in PCa.Although more and more studies are performed on NED in PCa recent decades,the molecular profiles that are implicated in NED are still poorly elucidated.Of these studies,signaling factors involved in genomic and microenvironmental components are deeply researched and most likely to provide the potential therapeutic targets for advanced PCa with NED.In this article,we review the hypothesis about the origin of NE cells and the molecular mechanisms driving NED from the point of genomic and environmental views.In addition,we discuss the current potential therapeutic targets and ongoing clinical trails associated with these molecular factors for the treatment of NED patients induced by ADT. 展开更多
关键词 Advanced PROSTATE cancer ANDROGEN DEPRIVATION therapy NEUROENDOCRINE differentiation
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基于分级顾客满意度的水果电商物流配送网络选址-路径问题 预览
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作者 陈诚 檀晓琳 邓颖 《华东交通大学学报》 2019年第4期88-94,共7页
互联网高速发展下,具有巨大市场潜能的水果电商市场不断吸引着投资者与消费者。在激烈的市场竞争下,顾客满意度是影响企业市场竞争力的重要因素之一。文章基于水果特性,以水果新鲜度和配送时效性为标准对顾客满意度进行了分析,建立了基... 互联网高速发展下,具有巨大市场潜能的水果电商市场不断吸引着投资者与消费者。在激烈的市场竞争下,顾客满意度是影响企业市场竞争力的重要因素之一。文章基于水果特性,以水果新鲜度和配送时效性为标准对顾客满意度进行了分析,建立了基于分级顾客满意度的选址-路径优化模型,并设计了相应的求解算法。最后,通过实例应用验证了该模型的可行性与有效性。 展开更多
关键词 水果电商 物流配送网络 顾客满意度 差异化
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抑制自噬对根尖乳头干细胞成骨分化水平的影响 预览
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作者 黄颖 熊华翠 +5 位作者 陈柯 朱晓斌 尹小萍 梁韵 罗薇 雷期音 《南方医科大学学报》 CAS CSCD 北大核心 2019年第1期106-112,共7页
目的探讨抑制自噬对根尖乳头干细胞成骨分化水平的影响。方法5、10ng/mL肿瘤坏死因子-α(TNF-α)分别处理根尖乳头干细胞(SCAPs),对照组不做处理,检测自噬相关蛋白LC3-II表达水平,GFP-LC3质粒转染并检测细胞内GFP-LC3数目,吖啶橙染色检... 目的探讨抑制自噬对根尖乳头干细胞成骨分化水平的影响。方法5、10ng/mL肿瘤坏死因子-α(TNF-α)分别处理根尖乳头干细胞(SCAPs),对照组不做处理,检测自噬相关蛋白LC3-II表达水平,GFP-LC3质粒转染并检测细胞内GFP-LC3数目,吖啶橙染色检测酸性囊泡情况。TNF-α,TNF-α+3-MA分别处理SCAPs,检测LC3-II的表达水平,GFP-LC3质粒转染并检测GFP-LC3数目,CCK-8法检测细胞活力,流式细胞仪检测细胞凋亡。TNF-α、TNF-α+3-MA分别处理SCAPs,对照组不做处理,诱导成骨向分化,qRT-PCR检测分化第3、7、14天时成骨相关基因碱性磷酸酶(ALP)、骨涎蛋白(BSP)、骨钙素(OCN)的表达。结果TNF-α可诱导SCAPs自噬活化:TNF-α组LC3-II/β-actin水平较对照组升高且具有浓度依赖性(P<0.05),TNF-α组GFP-LC3数目较对照组升高且具有浓度依赖性(P<0.05),TNF-α组酸性囊泡较对照组增多。3-MA可抑制TNF-α诱导的SCAPs自噬活化:TNF-α+3-MA组LC3-II/β-actin水平及细胞内GFP-LC3数目较TNF-α组显著降低(P<0.05),并下调细胞活力(P<0.05),上调细胞凋亡水平(P<0.05)。抑制自噬导致SCAPs成骨分化的抑制:TNF-α+3-MA组ALP、BSP表达量在分化第3、7、14天均较TNF-α组降低(P<0.05),OCN的表达量在第3、7天较TNF-α组降低(P<0.05)。结论TNF-α可诱导SCAPs自噬水平的活化;自噬可能对TNF-α作用下的SCAPs起细胞保护作用,对抗细胞凋亡;自噬的抑制将下调TNF-α作用下SCAPs的成骨向分化水平,提示自噬在TNF-α作用下SCAPs的成骨分化过程中具有重要作用。 展开更多
关键词 根尖乳头干细胞 肿瘤坏死因子-Α 自噬 分化
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集体林地征占用法律与事实的差异化分析
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作者 黄培锋 卢素兰 +1 位作者 黄和亮 杨伦增 《林业经济》 北大核心 2019年第8期30-36,43共8页
不同法律对林地征占用的表述不尽一致,由于政策执行不力,不可避免地造成了现行林地征占用法律与实践操作有偏差,极大地损害了林农在林地征收中的合法权益。文章借鉴Barzel和Demsetz的产权不完全界定理论,通过对"公共领域"概... 不同法律对林地征占用的表述不尽一致,由于政策执行不力,不可避免地造成了现行林地征占用法律与实践操作有偏差,极大地损害了林农在林地征收中的合法权益。文章借鉴Barzel和Demsetz的产权不完全界定理论,通过对"公共领域"概念进行拓展,揭示集体林地征占用法律与事实差异的深层次原因,探讨当前林地征占用实践所面临的困境,为制定和规范林地征占用法律政策提供一个基本方向。在此基础上,明确今后应进一步完善法律制度、完善征地补偿机制、健全法律法规、规范林地征收程序,保障被征林地农户的基本权益。 展开更多
关键词 集体林地 征占用 公共领域 差异性
城镇化进程中的小农户分化与升级 预览
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作者 吴业苗 《社会科学》 CSSCI 北大核心 2019年第9期74-84,共11页
城镇化进程中的小农户形态不稳定,正在加速分化,并随着现代农业发展和农业人口进城而转型升级。新时代小农户与大主体、大市场、大社会和大生产形成的组织、营销、社会和生产等结构性矛盾,加剧了小农户分化,致使越来越多的小农户放弃农... 城镇化进程中的小农户形态不稳定,正在加速分化,并随着现代农业发展和农业人口进城而转型升级。新时代小农户与大主体、大市场、大社会和大生产形成的组织、营销、社会和生产等结构性矛盾,加剧了小农户分化,致使越来越多的小农户放弃农业生产。如此,解决当下小农户深层次问题,除了在技术上促进小农户与现代农业发展衔接外,还要从空间再生产、生产方式再生产和生活方式再生产等方面着力提升小农户,促进小农户转化,减少小农户数量。唯有转化小农户并适度提升小农户,“在村”和正在或将要“离村”的小农户才能过上更美好的生活。 展开更多
关键词 小农户 结构性矛盾 分化 城镇化
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缺铁性贫血和地中海贫血常用血液实验室检查结果鉴别分析
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作者 祝少凤 梁桂兰 欧阳慧 《中国实用乡村医生杂志》 2019年第6期49-51,54共4页
目的对缺铁性贫血(IDA)和地中海贫血(TT)常用实验室血液检查结果进行比较,以期为临床鉴别诊断提供依据。方法回顾性收集清远市妇幼保健院门诊和住院部2011年1月-2018年5月收治的小细胞低色素性贫血患者186例,分为IDA组108例和TT组78例,... 目的对缺铁性贫血(IDA)和地中海贫血(TT)常用实验室血液检查结果进行比较,以期为临床鉴别诊断提供依据。方法回顾性收集清远市妇幼保健院门诊和住院部2011年1月-2018年5月收治的小细胞低色素性贫血患者186例,分为IDA组108例和TT组78例,比较两组患者血常规和骨髓、外周血涂片结果。结果两组红细胞计数、血红蛋白浓度、平均红细胞体积、平均红细胞血红蛋白浓度和红细胞分布宽度水平差异有统计学意义(P<0.05);而平均红细胞血红蛋白含量水平差异无统计学意义(P>0.05)。IDA组外周血和骨髓涂片靶形红细胞少见或未见;TT组靶形红细胞多见。骨髓铁染色下,IDA组细胞外铁和细胞内铁减少或缺乏,而TT组细胞外铁和细胞内铁增加。结论IDA和TT临床鉴别诊断存在一定困难,需要结合多种实验室检查以明确诊断。 展开更多
关键词 缺铁性贫血 地中海贫血 鉴别 诊断
Progesterone effects on the oligodendrocyte linage: all roads lead to the progesterone receptor 预览
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作者 Ignacio Jure Alejandro F. De Nicola Florencia Labombarda 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2029-2034,共6页
A new role has emerged for progesterone after discovering its potent actions away from reproduction in both the central and the peripheral nervous system. The aim of the present report is to discuss progesterone’s me... A new role has emerged for progesterone after discovering its potent actions away from reproduction in both the central and the peripheral nervous system. The aim of the present report is to discuss progesterone’s mechanisms of action involved in myelination, remyelination and neuroinflammation. The pivotal role of the classic progesterone receptor is described and evidence is compiled about progesterone’s direct effects on oligodendrocyte linage and its indirect effects on oligodendrocyte precursor cell differentiation by decreasing the neuroinflammatory environment. 展开更多
关键词 PROGESTERONE PROGESTERONE receptor OLIGODENDROCYTE differentiation MYELINATION REMYELINATION NEUROINFLAMMATION
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Effects of Ginkgo biloba extract EGb761 on neural differentiation of stem cells offer new hope for neurological disease treatment 预览
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作者 Chao Ren Yong-Qiang Ji +5 位作者 Hong Liu Zhe Wang Jia-Hui Wang Cai-Yi Zhang Li-Na Guan Pei-Yuan Yin 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1152-1157,共6页
Stem cell transplantation has brought new hope for the treatment of neurological diseases.The key to stem cell therapy lies in inducing the specific differentiation of stem cells into nerve cells.Because the different... Stem cell transplantation has brought new hope for the treatment of neurological diseases.The key to stem cell therapy lies in inducing the specific differentiation of stem cells into nerve cells.Because the differentiation of stem cells in vitro and in vivo is affected by multiple factors,the final differentiation outcome is strongly associated with the microenvironment in which the stem cells are located.Accordingly,the optimal microenvironment for inducing stem cell differentiation is a hot topic.EGb761 is extracted from the leaves of the Ginkgo biloba tree.It is used worldwide and is becoming one of the focuses of stem cell research.Studies have shown that EGb761 can antagonize oxygen free radicals,stabilize cell membranes,promote neurogenesis and synaptogenesis,increase the level of brain-derived neurotrophic factors,and replicate the environment required during the differentiation of stem cells into nerve cells.This offers the possibility of using EGb761 to induce the differentiation of stem cells,facilitating stem cell transplantation.To provide a comprehensive reference for the future application of EGb761 in stem cell therapy,we reviewed studies investigating the influence of EGb761 on stem cells.These started with the composition and neuropharmacology of EGb761,and eventually led to the finding that EGb761 and some of its important components play important roles in the differentiation of stem cells and the protection of a beneficial microenvironment for stem cell transplantation. 展开更多
关键词 nerve REGENERATION GINKGO biloba extract GINKGOLIDE B traditional Chinese medicine STEM cells induction of differentiation STEM cell transplantation synaptic plasticity pharmacological effect NEUROLOGICAL diseases nervous systems neural REGENERATION
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稳态磁场与干细胞
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作者 尚文龙 陈贵林 +2 位作者 卓渝娟 余鹰 任惠文 《生命的化学》 CAS CSCD 2019年第5期924-929,共6页
干细胞是一类具有自我复制能力和分化潜能的多功能细胞,这使得干细胞在医疗上的应用成为一种新颖而且有效的治疗方向。作为一种辅助治疗手段,稳态磁场可以影响牙髓干细胞、骨髓间充质干细胞、神经干细胞的增殖、迁移、分化和矿化等生理... 干细胞是一类具有自我复制能力和分化潜能的多功能细胞,这使得干细胞在医疗上的应用成为一种新颖而且有效的治疗方向。作为一种辅助治疗手段,稳态磁场可以影响牙髓干细胞、骨髓间充质干细胞、神经干细胞的增殖、迁移、分化和矿化等生理过程,有利于受损细胞的再生和受损组织的修复。磁靶向技术在干细胞中的应用研究进一步促进了稳态磁场在再生医学领域中的广泛应用,将干细胞有效地传递到靶部位,促进干细胞发挥免疫调节和修复等功能。本文就稳态磁场对干细胞的生物效应、作用机制和潜在应用进行了综述。 展开更多
关键词 稳态磁场 干细胞 干细胞治疗 增殖 迁移 分化
瘦素对人根尖乳头干细胞成骨/成牙本质相关基因表达的影响 预览
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作者 尹小萍 熊华翠 +2 位作者 陈柯 黄颖 徐帅妹 《口腔疾病防治》 2019年第1期23-29,共7页
目的探讨瘦素(leptin)对人根尖乳头干细胞(human stem cells from the apical papilla,hSCAPs)增殖及成骨/成牙本质相关基因表达的影响,为临床年轻恒牙根尖持续发育的研究提供实验基础。方法采用人根尖乳头组织块培养法获得hSCAPs,以免... 目的探讨瘦素(leptin)对人根尖乳头干细胞(human stem cells from the apical papilla,hSCAPs)增殖及成骨/成牙本质相关基因表达的影响,为临床年轻恒牙根尖持续发育的研究提供实验基础。方法采用人根尖乳头组织块培养法获得hSCAPs,以免疫荧光染色法、Western blot和qRT-PCR分别检测hSCAPs中leptin及其受体OBRb蛋白及基因的表达。实验组用质量分数为0.1μg/mL的leptin(0.1μg/mL组)和1.5μg/mL的leptin(1.5μg/mL组)分别刺激hSCAPs,对照组仅加入α-MEM培养基,CCK-8、流式细胞仪法、qRT-PCR分别检测各组hSCAPs增殖情况及成骨/成牙本质相关基因:碱性磷酸酶(alkaline phosphatase,ALP)、牙本质基质蛋白-1(dentin matrix protein-1,DMP-1)和牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)、骨钙素(osteocalcin,OCN)mRNA的表达。结果hSCAPs表达leptin及其受体OBRb的蛋白及基因。与对照组相比,实验组细胞增殖能力及S期细胞比例均高于对照组(P<0.05),且其中1.5μg/mL组高于0.1μg/mL组(P<0.05)。3 d和7 d时,0.1μg/mL组、1.5μg/mL组hSCAPs的ALP、DMP-1、DSPP mRNA的表达量高于对照组,且1.5μg/mL组高于0.1μg/mL组,差异有统计学意义(P<0.05),14 d时0.1μg/mL组、1.5μg/mL组hSCAPs的ALP、DMP-1、DSPP mRNA的表达量明显低于对照组(P<0.05);0.1μg/mL组、1.5μg/mL组OCN mRNA的表达量于7 d、14 d时高于对照组,且1.5μg/mL组均高于0.1μg/mL组,差异有统计学意义(P<0.05),于14 d达到峰值。结论Leptin可促进hSCAPs增殖,并上调hSCAPs成骨/成牙本质相关基因的表达。 展开更多
关键词 牙源性 根尖乳头干细胞 瘦素 成骨/成牙本质相关基因 分化 牙本质基质蛋白.1 牙本质涎磷蛋白 骨钙素
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Differentiation and pricing power of online retailers
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作者 Zhenjie Wang Chunling Zhu +1 位作者 Shanwu Tian Ping Li 《中国工商管理研究前沿:英文版》 2019年第1期101-120,共20页
This study aims to contribute to marketing theory by applying monopolistic competition theory to investigate how differentiation affects online retailers7 pricing power. We examine the intermediary effect of customer ... This study aims to contribute to marketing theory by applying monopolistic competition theory to investigate how differentiation affects online retailers7 pricing power. We examine the intermediary effect of customer loyalty on the relationship between differentiation and pricing power of online retailers. We assume that customers have different preferences for the differentiated characteristics of online retailers, and thus different degrees of willingness to pay premiums, which gives online retailers pricing power. Structural equation modeling (SEM) is employed to test the hypothesized relati on ships between differentiation, customer loyalty, and pricing power. The statistical results of the empirical data indicate that some of the differentiation characteristics of online retailers can cultivate their pricing power indirectly. Logistics quality followed by commodity assortment and transaction security produces profound effects on the buildup of pricing power with customer loyalty as an intermediary factor. Results further reveal that commodity quality is positively related to pricing power. This study expands the definition of differentiation by combining corporate strategic positioning with commodity pricing. Implications for practice and di recti ons for future research are provided. 展开更多
关键词 Online RETAILER DIFFERENTIATION Pricing power Customer LOYALTY Logistics quality COMMODITY ASSORTMENT TRANSACTION security
Traditional Chinese medicine nursing protocols for acute attack of hepatolithiasis 预览
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作者 Editorial Board of Nursing of Integrated Traditional Chinese and Western Medicine 《中西医结合护理(中英文)》 2019年第2期229-234,共6页
Hepatolithiasis is characterized by the presence of stones within the intrahepatic bile ducts proximal to the right and left hepatic ducts. It is universally recognized that the occurrence of hepatolithiasis is closel... Hepatolithiasis is characterized by the presence of stones within the intrahepatic bile ducts proximal to the right and left hepatic ducts. It is universally recognized that the occurrence of hepatolithiasis is closely related to cholangitis, cholestasis, abnormal bile metabolism, anatomical abnormality of bile ducts, malnutrition and low socio-economic status. Surgery is the primary treatment method for hepatolithiasis in clinic, but some patients may still suffer from recurrence or complications after surgery. Traditional Chinese medicine(TCM) has its own unique advantages in preventing and treating recurrence of calculi. This article mainly explored the key points of common syndromes, TCM nursing methods and health guidance of acute attack of hepatolithiasis in order to further develop the advantages of TCM, improve its efficacy and standardized its nursing behavior. 展开更多
关键词 HEPATOLITHIASIS acute ATTACK JAUNDICE CONSTIPATION traditional Chinese medicine NURSING syndrome DIFFERENTIATION
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山羊胎肝内人脐带间充质干细胞的存活与分化研究 预览
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作者 鄢东海 黄小昆 +6 位作者 向雪萍 卿德科 方伟 李自安 王强 王金祥 庞荣清 《实验动物与比较医学》 CAS 2019年第5期364-370,共7页
目的观察人脐带间充质干细胞(hUCMSC)在胎羊肝脏内的存活与分化。方法妊娠2个月时在胎羊肝脏实质区注射绿色荧光蛋白(GFP)转染标记的hUCMSC(GFP-hUCMSC),出生1月龄时取羔羊肝脏组织,制作切片后在荧光显微镜下观察GFP阳性细胞的分布,采用... 目的观察人脐带间充质干细胞(hUCMSC)在胎羊肝脏内的存活与分化。方法妊娠2个月时在胎羊肝脏实质区注射绿色荧光蛋白(GFP)转染标记的hUCMSC(GFP-hUCMSC),出生1月龄时取羔羊肝脏组织,制作切片后在荧光显微镜下观察GFP阳性细胞的分布,采用PCR方法检测人雄性性别决定基因(SRY)的存在;RT-PCR方法检测人肝细胞特异基因的表达;免疫组织化学染色检测人肝细胞特异蛋白的表达。注射同体积生理盐水的同月龄羔羊为对照。结果在注射了GFP-hUCMSC的1月龄羔羊肝脏组织中可观察到绿色荧光的规则或散在分布,并可检测到人SRY基因的存在。RT-PCR方法可检测到人肝细胞核因子4α(HNF4α)、肝细胞核因子3β(HNF3β)和白蛋白(ALB)基因的阳性表达;免疫组织化学染色可检测到人ALB和HNF4α蛋白的阳性表达,而对照羔羊肝脏检测不到绿色荧光阳性细胞和人SRY基因以及人肝细胞特异基因和蛋白的表达。结论hUCMSC可以在胎羊肝脏内存活并分化为人肝细胞样细胞,并可在胎羊出生后至少持续一个月。 展开更多
关键词 人脐带间充质干细胞(hUCMSC) 山羊 肝细胞 分化
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