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Plasma relative abundance of epidermal growth factor receptor mutations predicts clinical response to epidermal growth factor receptor-tyrosine kinase inhibitors in patients with advanced lung adenocarcinoma
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作者 徐含烟 《中国医学文摘:内科学分册(英文版)》 2019年第2期103-104,共2页
Objective To determine whether relative abundance of epidermal growth factor receptor (EGFR) mutations in plasma predicts clinical response to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) in... Objective To determine whether relative abundance of epidermal growth factor receptor (EGFR) mutations in plasma predicts clinical response to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) in patients with advanced lung adenocarcinoma. Methods In this prospective study,adult patients with advanced lung adenocarcinoma were enrolled in our hospital from 1 April 2016 to 1 January 2017. EGFR mutations in tumortissues were detected by ADx-amplification refractory mutationsystem (ADx-ARMS). EGFR mutations of plasmafree tumor DNA were detected by ADx-ARMS and ADxsuperamplification refractory mutation system (ADx-SuperARMS)at the same time. Patients with EGFR-mutantin tumor tissues and receiving EGFR-TKIs were finallyenrolled. Plasma mutation-positive patients with bothmethods were high abundance group. Patients with positivemutations by ADx-SuperARMS but negative of ADx-ARMS were medium abundance group. 展开更多
关键词 PFS PLASMA relative abundance of EPIDERMAL GROWTH FACTOR RECEPTOR mutations predicts clinical response to EPIDERMAL GROWTH FACTOR receptor-tyrosine kinase inhibitors in PATIENTS with advanced lung adenocarcinoma
New approaches to cognitive work analysis through latent variable modeling in mining operations 预览
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作者 S.Li Y.A.Sari M.Kumral 《矿业科学技术学报:英文版》 CSCD 2019年第4期549-556,共8页
This paper discusses the utilization of latent variable modeling related to occupational health and safety in the mining industry.Latent variable modeling,which is a statistical model that relates observable and laten... This paper discusses the utilization of latent variable modeling related to occupational health and safety in the mining industry.Latent variable modeling,which is a statistical model that relates observable and latent variables,could be used to facilitate researchers’understandings of the underlying constructs or hypothetical factors and their magnitude of effect that constitute a complex system.This enhanced understanding,in turn,can help emphasize the important factors to improve mine safety.The most commonly used techniques include the exploratory factor analysis(EFA),the confirmatory factor analysis(CFA)and the structural equation model with latent variables(SEM).A critical comparison of the three techniques regarding mine safety is provided.Possible applications of latent variable modeling in mining engineering are explored.In this scope,relevant research papers were reviewed.They suggest that the application of such methods could prove useful in mine accident and safety research.Application of latent variables analysis in cognitive work analysis was proposed to improve the understanding of human-work relationships in mining operations. 展开更多
关键词 Latent variables Exploratory factor analysis Confirmatory factor analysis Structural equation modeling Occupational health and safety Mine safety
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Diabetic neuropathy research: from mouse models to targets for treatment 预览
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作者 Vuong M. Pham Shinji Matsumura +2 位作者 Tayo Katano Nobuo Funatsu Seiji Ito 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1870-1879,共10页
Diabetic neuropathy is one of the most serious complications of diabetes, and its increase shows no sign of stopping. Furthermore, current clinical treatments do not yet approach the best effectiveness. Thus, the deve... Diabetic neuropathy is one of the most serious complications of diabetes, and its increase shows no sign of stopping. Furthermore, current clinical treatments do not yet approach the best effectiveness. Thus, the development of better strategies for treating diabetic neuropathy is an urgent matter. In this review, we first discuss the advantages and disadvantages of some major mouse models of diabetic neuropathy and then address the targets for mechanism-based treatment that have been studied. We also introduce our studies on each part. Using stem cells as a source of neurotrophic factors to target extrinsic factors of diabetic neuropathy, we found that they present a promising treatment. 展开更多
关键词 brain derived NEUROTROPHIC FACTOR DIABETES extrinsic FACTORS NERVE growth FACTOR NERVE regeneration NEUROTROPHIC FACTORS NON-OBESE type 2 DIABETES phosphatase and tensin homolog stem cell streptozotocin
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Assessment of seismic amplifi cation factor of excavation with support system 预览
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作者 Hamidreza Tavakoli Saman Soleimani Kutanaei Seyed Hossein Hosseini 《地震工程与工程振动:英文版》 SCIE EI CSCD 2019年第3期555-566,共12页
Retaining walls have been used in many construction projects such as for road and inclined surfaces protection. The damage caused by an earthquake depends on the fundamental frequency, amplitude and the duration of th... Retaining walls have been used in many construction projects such as for road and inclined surfaces protection. The damage caused by an earthquake depends on the fundamental frequency, amplitude and the duration of the seismic motion. These parameters strongly depend on the seismic properties of the layers that are near the surface. In the study of retaining walls, in addition to the infl uence of soil, the infl uence of topography is also important. In the present study, site response analysis is performed by using fi nite element software PLAXIS to obtain the eff ect of various factors such as embedded length of the sheet pile, underground water table, length and angle of the nail, shear wave velocity of soil on site eff ect and dynamic response. Moreover, for better understanding of the eff ect of the above parameters, the stability analysis was performed by using shear reduction method. The results show that an increase in the embedded length of the sheet pile and the length of nailing causes an increase in the amplifi cation factor. Moreover, for shear-wave velocity in the range of 200- 600 m/s, the amplifi cation factor increases with increase of the shear-wave velocity due to the decrease of nonlinear behavior. 展开更多
关键词 amplifi CATION FACTOR safety FACTOR shear wave velocity NAILING sheet PILE
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A Meta-Analysis on Phenotypic Variation in Cadmium Accumulation of Rice and Wheat:Implications for Food Cadmium Risk Control
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作者 LI Xiaofang ZHOU Dongmei 《土壤圈:英文版》 CSCD 2019年第5期545-553,共9页
In some densely-populated countries, farmland has been widely cadmium (Cd) contaminated, and the utilization of the contaminated farmland for crop production is currently unavoidable. This necessitates the use of low-... In some densely-populated countries, farmland has been widely cadmium (Cd) contaminated, and the utilization of the contaminated farmland for crop production is currently unavoidable. This necessitates the use of low-Cd crops (i.e., pollution-safe cultivars, the crop varieties with the ability to accumulate a low level of Cd in their edible parts when grown on polluted soil) in these areas and highlights the importance of knowledge on phenotypic variation in crop Cd accumulation for food Cd risk control. Studies on phenotypic variation in heavy metal accumulation started decades ago for a wide range of crops, and synthesis of the scattered experimental results in the literature is in need. We built a Low-Cd Crops Database based on literature research, and relevant meta-analysis was performed to quantitatively explore the phenotypic variation in Cd uptake and translocation of rice and wheat. Considerable variability existed among rice (median grain Cd bioconce nt ration factor (BCF) of 0.10) and wheat (median grain Cd BCF of 0.21) phenotypes in grain Cd accumulation, and this variability was labile to soil pH and the level of Cd stress. Wheat statistically had a higher root-to-shoot Cd-translocating ability than rice, highlighting potential food Cd risks and the importance of growing low-Cd wheat in slightly Cd-contaminated regions. Meanwhile, no correlations were detected among soil-to-root, root-to-shoot, and shoot-to-grain translocation factors, implying that Cd uptake and internal translocation in crops were probably controlled by different underlying gene tic mechanisms. Root-to-shoot Cd transport could be a favorable target trait for selecting and breeding low-Cd rice and wheat. In all, this review provides a comprehensive low-Cd crop list for remediation practice and a systematic meta-analysis inferring food Cd risks based on plant capacity for Cd accumulation and desired traits for low-Cd crop breeding. 展开更多
关键词 BIOCONCENTRATION FACTOR CD contamination CD stress heavy metal low-Cd CROPS plant uptake pollution-safe CULTIVARS soil pH TRANSLOCATION FACTOR
Pollen Typhae Total Flavone Inhibits Endoplasmic Reticulum Stress-Induced Apoptosis in Human Aortic-Vascular Smooth Muscle Cells through Down-Regulating PERK-eIF2α-ATF4-CHOP Pathway
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作者 CHEN Ming-tai HUANG Ruo-lan +6 位作者 OU Li-jun CHEN Ying-nan MEN Ling CHANG Xiao WANG Ling YANG Yu-zhu ZHANG Zhong 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第8期604-612,共9页
Objective: To test the hypothesis that the inhibition of endoplasmic reticulum(ER) stress-induced apoptosis in oxidized low-density lipoproteins(ox-LDL)-induced human aortic-vascular smooth muscle cells(HA-VSMCs) was ... Objective: To test the hypothesis that the inhibition of endoplasmic reticulum(ER) stress-induced apoptosis in oxidized low-density lipoproteins(ox-LDL)-induced human aortic-vascular smooth muscle cells(HA-VSMCs) was associated with suppression of the protein kinase RNA-like ER kinase(PERK)-eukaryotic translation initiation factor 2α(e IF2α)-activating transcription factor 4(ATF4)-CCAAT/enhancer binding protein homologous protein(CHOP) signaling pathway by Pollen Typhae total flavone(PTF). Methods: Primary HA-VSMCs were cultured and identified. The cultured HA-VSMCs were randomized into 5 groups, including a normal control group, an ox-LDL group(70 μg/m L high ox-LDL), an HPTF group(70 μg/m L high ox-LDL+500 μg/m L PTF), an MPTF group(70 μg/m L high ox-LDL+250 μg/m L PTF), and a LPTF group(70 μg/m L high ox-LDL+100 μg/m L PTF) in the first part;and a normal control group, an ox-LDL group(70 μg/mL high ox-LDL), an MPTF group(70 μg/m L high ox-LDL+250 μg/m L PTF), a sh RNA group(transducted with PERK shRNA lentiviral particles), a scramble shRNA group(transducted with control shRNA lentiviral particles), an MPTF+ox-LDL+shRNA group(250 μg/mL PTF+70 μg/mL high ox-LDL+PERK shRNA lentiviral particles) and an ox-LDL+shRNA group(70 μg/mL high ox-LDL+PERK shRNA lentiviral particles) in the second part. The protein expression levels of ER-associated apoptosis proteins were detected by Western blot, and their m RNA expression levels were detected by quantitative real-time reverse transcription-polymerase chain reaction. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was applied to test cel viability, and the level of apoptosis was monitored by flow cytometry. Results: The MTT assay and flow cytometry showed that the ox-LDL group had a significant increase in apoptosis, which was attenuated in PTF treatment groups and sh RNA groups. Moreover, the ox-LDL group had increased protein and m RNA levels of binding immunoglobulin protein and ER-associated apoptosis proteins, such as PERK, 展开更多
关键词 Pollen Typhae total flavone endoplasmic reticulum stress PROTEIN KINASE RNA-like endoplasmic reticulum kinase-eukaryotic translation initiation FACTOR 2a -activating transcription FACTOR 4-CCAAT/enhancer binding PROTEIN homologous PROTEIN PATHWAY apoptosis vulnerable atherosclerotic plaque
Early treadmill exercise increases macrophage migration inhibitory factor expression after cerebral ischemia/reperfusion 预览
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作者 Min Cheol Chang Chae Ri Park +2 位作者 Seung Hwa Rhie Woo Hyun Shim Dae Yul Kim 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1230-1236,共7页
The neuroprotective function of macrophage migration inhibitory factor(MIF)in ischemic stroke was rarely evaluated.This study aimed to investigate the effects of early treadmill exercise on recovery from ischemic stro... The neuroprotective function of macrophage migration inhibitory factor(MIF)in ischemic stroke was rarely evaluated.This study aimed to investigate the effects of early treadmill exercise on recovery from ischemic stroke and to determine whether these effects are associated with the expression levels of MIF and brain-derived neurotrophic factor(BDNF)in the ischemic area.A total of 40 male Sprague-Dawley rats were randomly assigned to the ischemia and exercise group[middle cerebral artery occlusion(MCAO)-Ex,n=10),ischemia and sedentary group(MCAO-St,n=10),sham-surgery and exercise group(Sham-Ex,n=10),or sham-surgery and sedentary group(Sham-St,n=10).The MCAO-Ex and MCAO-St groups were subjected to MCAO for 60 minutes,whereas the Sham-Ex and Sham-St groups were subjected to an identical operation without MCAO.Rats in the MCAO-Ex and Sham-Ex groups then ran on a treadmill for 30 minutes once a day for 5 consecutive days.After reperfusion,the hanging time tested by the wire hang test was longer and the relative fractional anisotropy determined by MRI was higher in the peri-infarct region of the MCAO-Ex group compared with the MCAO-St group.The expression levels of MIF and BDNF in the peri-infarct region were upregulated in the MCAO-Ex group.Increased MIF and BDNF levels were positively correlated with relative fractional anisotropy changes in the peri-infarct region.There was no significant difference in the levels of MIF and BDNF in the peri-infarct region between the Sham-Ex and Sham-St groups.Our study demonstrated that early exercise(initiated 48 hours after the MCAO)could improve motor and neuronal recovery after ischemic stroke.Furthermore,the increased levels of MIF and BDNF in the peri-infarct region(penumbra)may be one of the mechanisms of enhanced neurological function recovery.All experiments were approved by the Institutional Animal Care and Use Committee in Asan Medical Center in South Korea(2016-12-126). 展开更多
关键词 ischemic stroke EARLY exercise macrophage migration inhibitory FACTOR BRAIN-DERIVED NEUROTROPHIC FACTOR motor recovery neural regeneration
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Sources and Ecological Risks of Heavy Metals in Soils Under Different Land Uses in Bangladesh
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作者 Mohammad Saiful ISLAM Mohammad Kawser AHMED +1 位作者 Mohammad Habibullah AL-MAMUN Shah Mohammad Asraful ISLAM 《土壤圈:英文版》 CSCD 2019年第5期665-675,共11页
Soil heavy metal pollution, influenced by both natural and anthropogenic factors, significantly reduces environmental quality. In this study, Cr, Ni, Cu, As, Cd, and Pb in eight different land-use soils from Patuakhal... Soil heavy metal pollution, influenced by both natural and anthropogenic factors, significantly reduces environmental quality. In this study, Cr, Ni, Cu, As, Cd, and Pb in eight different land-use soils from Patuakhali District in Bangladesh were assessed. Concentrations of Cr, Ni, Cu, As, Cd, and Pb in soils were 1-87, 5-271, 4-181, 0-80, 0.2-24.0, and 5-276 mg kg-1, respectively, measured using an inductively coupled plasma-mass spectrometer. The enrichment factor, pollution load index (PLI), and contamination factor (CJ) of metal i were used to assess the ecological risk posed by metals in soils. The PLI ranged from 0.78 to 2.66, indicating baseline levels to progressive deterioration of soil due to metal contamination. However, C;of Cd ranged from 1.8 to 12.0, which showed that the studied soils were strongly impacted by Cd. Considering the severity of the potential ecological risk of a single metal, the descending order was Cd > As > Pb > Cu > Ni > Cr. Soils under all land uses showed moderate to very high potential ecological risk. 展开更多
关键词 Cd CONTAMINATION FACTOR degree of CONTAMINATION enrichment FACTOR environmental quality POLLUTION load index SOIL DETERIORATION SOIL POLLUTION
曲古抑菌素A预处理对小鼠脑缺血再灌注损伤皮质炎症反应和凋亡的影响 预览
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作者 侯家保 袁泉 +3 位作者 万杏 刘恋 赵博 吴洋 《中国现代医学杂志》 CAS 2019年第9期1-5,共5页
目的探讨曲古抑菌素A预处理在小鼠脑缺血再灌注损伤中对大脑皮质炎症反应和凋亡的影响。方法Balb/c小鼠随机分为3组:假手术组(S组)、缺血再灌注组(IR组)、曲古抑菌素A预处理组(预处理组),每组10只。采用颈部切口大脑中动脉线栓(MCAO)法... 目的探讨曲古抑菌素A预处理在小鼠脑缺血再灌注损伤中对大脑皮质炎症反应和凋亡的影响。方法Balb/c小鼠随机分为3组:假手术组(S组)、缺血再灌注组(IR组)、曲古抑菌素A预处理组(预处理组),每组10只。采用颈部切口大脑中动脉线栓(MCAO)法缺血1h,再灌注24h复制脑缺血再灌注模型,预处理组在复制脑缺血再灌注模型前连续3d腹腔注射曲古抑菌素A5mg/kg。取脑皮质组织,光学显微镜下观察病理学结果,ELISA检测TNF-α、IL-1β,免疫组织化学法检测Bcl-2、Bax、Caspase-3,TUNEL检测细胞凋亡。结果3组TNF-α、IL-1β、Bax、Bcl-2及Caspase-3比较,差异有统计学意义(P<0.05)。与S组比较,IR组病理学损伤严重,TNF-α、IL-1β、Bax、Caspase-3阳性表达水平升高,Bcl-2降低(P<0.05);与IR组比较,预处理组病理学损伤减轻,TNF-α、IL-1β、Bax、Caspase-3阳性表达水平降低,Bcl-2升高(P<0.05)。结论曲古抑菌素A预处理能抑制炎症因子、凋亡因子的表达及减少细胞凋亡,从而减轻脑缺血再灌注损伤。 展开更多
关键词 再灌注损伤 模型 动物 曲古抑菌素A 因子 炎症 凋亡诱导因子
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Microglial cathepsin B as a key driver of inflammatory brain diseases and brain aging 预览
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作者 Hiroshi Nakanishi 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第1期25-29,共5页
Interleukin-1βis a potent proinflammatory cytokine that plays a key role in the pathogenesis of the brain aging and diverse range of neurological diseases including Alzheimer’s disease,Parkinson’s disease,stroke an... Interleukin-1βis a potent proinflammatory cytokine that plays a key role in the pathogenesis of the brain aging and diverse range of neurological diseases including Alzheimer’s disease,Parkinson’s disease,stroke and persistent pain.Activated microglia are the main cellular source of interleukin-1βin the brain.Cathepsin B is associated with the production and secretion of interleukin-1βthrough pyrin domain-containing protein 3 inflammasome-independent processing of procaspase-3 in the phagolysosomes.The leakage of cathepsin B from the endosomal-lysosomal system during aging is associated with the proteolytic degradation of mitochondrial transcription factor A,which can stabilize mitochondrial DNA.Therefore,microglial cathepsin B could function as a major driver for inflammatory brain diseases and brain aging.Orally active and blood-brain barrier-permeable specific inhibitors for cathepsin B can be potentially effective new pharmaceutical interventions against inflammatory brain diseases and brain aging. 展开更多
关键词 brain aging CASPASE-1 cathepsin B inflammatory brain diseases INTERLEUKIN-1Β MICROGLIA mitochondrial transcription factor A NEUROINFLAMMATION nuclear factor-κB oxidative stress
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Hyodeoxycholic acid protects the neurovascular unit against oxygen-glucose deprivation and reoxygenation-induced injury in vitro 预览
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作者 Chang-Xiang Li Xue-Qian Wang +3 位作者 Fa-Feng Cheng Xin Yan Juan Luo Qing-Guo Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1941-1949,共9页
Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin an... Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin and jas-minoidin, HDCA prevents hypoxia-reoxygenation-induced brain injury by suppressing endoplasmic reticulum stress-mediated apoptotic signaling. However, the effects of HDCA in ischemic stroke injury have not yet been studied. Neurovascular unit(NVU) dysfunction occurs in ischemic stroke. Therefore, in this study, we investigated the effects of HDCA on the NVU under ischemic conditions in vitro. We co-cultured primary brain microvascular endothelial cells, neurons and astrocytes using a transwell chamber co-culture system. The NVU was pre-treated with 10.16 or 2.54 μg/mL HDCA for 24 hours before exposure to oxygen-glucose deprivation for 1 hour. The cell counting kit-8 assay was used to detect cell activity. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling were used to assess apoptosis. Enzyme-linked immunosorbent assay was used to measure the expression levels of inflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor-α, and neurotrophic factors, including brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. Oxidative stress-related factors, such as superoxide dismutase, nitric oxide, malondialdehyde and γ-glutamyltransferase, were measured using kits. Pretreatment with HDCA significantly decreased blood-brain barrier permeability and neuronal apoptosis, significantly increased transendothelial electrical resistance and γ-glutamyltransferase activity, attenuated oxidative stress damage and the release of inflammatory cytokines, and increased brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression. Our findings suggest that HDCA maintains NVU morphological integrity and function by modulating inflammation, oxidation stress, apoptosis, and the expression o 展开更多
关键词 hyodeoxycholic acid oxygen glucose deprivation and REOXYGENATION blood-brain barrier permeability anti-oxidative anti-inflammatory ANTI-APOPTOTIC BRAIN-DERIVED NEUROTROPHIC FACTOR glial cell line-derived NEUROTROPHIC FACTOR ischemic stroke in vitro NEUROVASCULAR unit
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Vascular endothelial growth factor A promotes platelet adhesion to collagen Ⅳ and causes early brain injury after subarachnoid hemorrhage 预览
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作者 Zun-Wei Liu Jun-Jie Zhao +1 位作者 Hong-Gang Pang Jin-Ning Song 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1726-1733,共8页
The role of vascular endothelial growth factor A in platelet adhesion in cerebral microvessels in the early stage of subarachnoid hemorrhage remains unclear.In this study,the endovascular puncture method was used to p... The role of vascular endothelial growth factor A in platelet adhesion in cerebral microvessels in the early stage of subarachnoid hemorrhage remains unclear.In this study,the endovascular puncture method was used to produce a rat model of subarachnoid hemorrhage.Then,30 minutes later,vascular endothelial growth factor A antagonist anti-vascular endothelial growth factor receptor 2 antibody,10μg,was injected into the right ventricle.Immunohistochemistry and western blot assay were used to assess expression of vascular endothelial growth factor A,occludin and claudin-5.Immunohistochemical double labeling was conducted to examine co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen.TUNEL was used to detect apoptosis in the hippocampus.Neurological score was used to assess behavioral performance.After subarachnoid hemorrhage,the expression of vascular endothelial growth factor A increased in the hippocampus,while occludin and claudin-5 expression levels decreased.Co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen and the number of apoptotic cells increased,whereas behavioral performance was markedly impaired.After treatment with anti-vascular endothelial growth factor receptor 2 antibody,occludin and claudin-5 expression recovered,while co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen and the number of apoptotic cells decreased.Furthermore,behavioral performance improved notably.Our findings suggest that increased vascular endothelial growth factor A levels promote platelet adhesion and contribute to early brain injury after subarachnoid hemorrhage.This study was approved by the Biomedical Ethics Committee,Medical College of Xi’an Jiaotong University,China in December 2015. 展开更多
关键词 nerve REGENERATION VASCULAR ENDOTHELIAL GROWTH FACTOR A VASCULAR ENDOTHELIAL GROWTH FACTOR receptor 2 subarachnoid hemorrhage brain injuries platelet adhesion COLLAGEN blood-brain barrier neural REGENERATION
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Effects of Huatan Tongluo decoction on vascular endothelial growth factor receptor 2 expression in synovial tissues of rats with collagen-induced arthritis
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作者 Chen Jinchun Qiu Mingshan +7 位作者 Li Yihan Zhang Qian Zhang Yiyan Lin Shuangjie Zhang Shaohong Qian Lixia Gao Hai Li Liang-cheng 《中医杂志:英文版》 SCIE CAS CSCD 2019年第2期191-198,共8页
OBJECTIVE:To determine the therapeutic effect and potential mechanism of Huatan Tongluo decoction on rats with collagen-induced arthritis.METHODS:Forty specific pathogen-free Wistar rats were selected,and 10 were rand... OBJECTIVE:To determine the therapeutic effect and potential mechanism of Huatan Tongluo decoction on rats with collagen-induced arthritis.METHODS:Forty specific pathogen-free Wistar rats were selected,and 10 were randomly selected as the control(group 1).The remaining rats were injected intradermally with emulsified type II bovine collagen at the tail base and back,followed by a booster 7 d post first immunization.After establishing collagen-induced arthritis(CIA),rats were randomly divided into three groups(n=10).The rats were treated orally for 30 d as follows:group 1,saline;group 2,model(saline);group 3,tripterygium polyglycoside(TP;7.81 mg/kg,positive control);group 4,Huatan Tongluo decoction(HTTL;7.5 g/kg).Body weight,ankle swelling and arthritis index were measured over the course of the study.The rats were sacrificed 30 d after treatment.Morphological changes in the synovium were observed by hematoxylin and eosin staining.Pannus formation and synovial thickness in the left ankle were observed by color Doppler ultrasoundVascular endothelial growth factor(VEGF)and VEGFR2 protein levels were measured by immunohistochemistry.VEGF/VEGFR2 mRNA levels were measured by real-time quantitative polymerase chain reaction.RESULTS:Compared with the model group,a significantly lower arthritis index was observed in the positive control group(P<0.05)and HTTL group(P<0.01),after treatment.Both positive control and HTTL reduced intra-articular pannus formation and synovial thickening.Furthermore,VEGF mRNA,and VEGFR2 protein and mRNA levels were significantly downregulated(P<0.05)in the treatment groups.CONCLUSION:Inhibition of the expression of VEGF and VEGFR2 in synovial tissues and the formation of pannus and synovial hyperplasia may be part of the mechanism of HTTL for relieving the symptoms of rheumatoid arthritis in CIA rats. 展开更多
关键词 Arthritis experimental TRIPTERYGIUM VASCULAR ENDOTHELIAL GROWTH FACTOR A VASCULAR ENDOTHELIAL GROWTH FACTOR receptor-2 Huatan Tongluo decocti on
Acacetin protects against cerebral ischemiareperfusion injury via the NLRP3 signaling pathway 预览
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作者 Juan Bu Shen Shi +8 位作者 Hui-Qin Wang Xiao-Shan Niu Zong-Feng Zhao Wei-Dong Wu Xiao-Ling Zhang Zhi Ma Yan-Jun Zhang Hui Zhang Yi Zhu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期605-612,共8页
Acacetin(5,7-dihydroxy-4′-methoxyflavone),a potential neuroprotective agent,has an inhibitory effect on lipopolysaccharide-induced neuroinflammatory reactions.However,whether acacetin has an effect on inflammatory co... Acacetin(5,7-dihydroxy-4′-methoxyflavone),a potential neuroprotective agent,has an inhibitory effect on lipopolysaccharide-induced neuroinflammatory reactions.However,whether acacetin has an effect on inflammatory corpuscle 3(NLRP3)after cerebral ischemia-reperfusion injury has not been fully determined.This study used an improved suture method to establish a cerebral ischemia-reperfusion injury model in C57BL/6 mice.After ischemia with middle cerebral artery occlusion for 1 hour,reperfusion with intraperitoneal injection of 25 mg/kg of acacetin(acacetin group)or an equal volume of saline(0.1 mL/10 g,middle cerebral artery occlusion group)was used to investigate the effect of acacetin on cerebral ischemia-reperfusion injury.Infarct volume and neurological function scores were determined by 2,3,5-triphenyltetrazolium chloride staining and the Zea-Longa scoring method.Compared with the middle cerebral artery occlusion group,neurological function scores and cerebral infarction volumes were significantly reduced in the acacetin group.To understand the effect of acacetin on microglia-mediated inflammatory response after cerebral ischemia-reperfusion injury,immunohistochemistry for the microglia marker calcium adapter protein ionized calcium-binding adaptor molecule 1(Iba1)was examined in the hippocampus of ischemic brain tissue.In addition,tumor necrosis factor-α,interleukin-1β,and interleukin-6 expression in ischemic brain tissue of mice was quantified by enzyme-linked immunosorbent assay.Expression of Iba1,tumor necrosis factor-α,interleukin-1βand interleukin-6 was significantly lower in the acacetin group compared with the middle cerebral artery occlusion group.Western blot assay results showed that expression of Toll-like receptor 4,nuclear factor kappa B,NLRP3,procaspase-1,caspase-1,pro-interleukin-1β,and interleukin-1βwere significantly lower in the acacetin group compared with the middle cerebral artery occlusion group.Our findings indicate that acacetin has a protective effect on cerebral ischemia-repe 展开更多
关键词 nerve REGENERATION ACACETIN cerebral ischemia-reperfusion injury microglia NLRP3 INFLAMMASOME inflammatory FACTOR INFARCT volume signaling pathway nuclear factor-κB neuroprotection neural REGENERATION
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Carbon productivity convergence club and its initial conditions: China's construction industry 预览
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作者 Puwei Zhang Guangshe Jiaa +3 位作者 Qiang Mou Mingli Song Changquan He Qixiong Xu 《中国人口资源与环境:英文版》 2019年第1期12-24,共13页
A three-stage method is proposed to study the convergence clubs for the dynamic total factor carbon productivity (DCP) and the initial conditions. The first stage is to measure the DCP that reflects the initial differ... A three-stage method is proposed to study the convergence clubs for the dynamic total factor carbon productivity (DCP) and the initial conditions. The first stage is to measure the DCP that reflects the initial difference. The second stage is to identify the convergence club of DCP. The last stage is to examine the initial factors that may affect the formation of the convergence club. Construction industry data from 30 provinces in mainland China from 2005 to 2016 were adopted to conduct an empirical study. The empirical results showed that (1) the arithmetic mean value of China’s provincial DCP showed an upward trend and the standard deviation showed an expanding trend.(2) There are five convergence clubs, but 13 provinces failed to converge to any club.(3) The higher the degree of construction industry marketization in 2005, the greater the probability that the provinces belong to a club with higher DCP. To improve the DCP, the effective diffusion of low-carbon construction technologies and the market-oriented reform of state-owned construction companies should be promoted. The three-stage method can also be applied to study different industries in different countries or regions. 展开更多
关键词 Total FACTOR carbon productivity CLUB convergence construction industry nonlinear TIME-VARYING FACTOR MODEL ordered LOGIT regression MODEL
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Berberine prevents stress-induced gut inflammation and visceral hypersensitivity and reduces intestinal motility in rats 预览
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作者 Zhi-Chao Yu Yong-Xin Cen +10 位作者 Ben-Hua Wu Cheng Wei Feng Xiong De-Feng Li Ting-Ting Liu Ming-Han Luo Li-Liangzi Guo Ying-Xue Li Li-Sheng Wang Jian-Yao Wang Jun Yao 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第29期3956-3971,共16页
BACKGROUND Irritable bowel syndrome (IBS) is a common chronic non-organic disease of the digestive system. Berberine (BBR) has been used to treat patients with IBS, but the underlying therapeutic mechanism is little u... BACKGROUND Irritable bowel syndrome (IBS) is a common chronic non-organic disease of the digestive system. Berberine (BBR) has been used to treat patients with IBS, but the underlying therapeutic mechanism is little understood. We believe that BBR achieves its therapeutic effect on IBS by preventing stress intestinal inflammation and visceral hypersensitivity and reducing bowel motility. AIM To test the hypothesis that BBR achieves its therapeutic effect on IBS by preventing subclinical inflammation of the intestinal mucosa and reducing visceral hypersensitivity and intestinal motility. METHODS IBS was induced in rats via water avoidance stress (WAS). qRT-PCR and histological analyses were used to evaluate the levels of cytokines and mucosal inflammation, respectively. Modified ELISA and qRT-PCR were used to evaluate the nuclear factor kappa-B (NF-κB) signal transduction pathway. Colorectal distention test, gastrointestinal transit measurement, Western blot, and qRT-PCR were used to analyze visceral sensitivity, intestinal motility, the expression of Ckit (marker of Cajal mesenchymal cells), and the expression of brain derived neurotrophic factor (BDNF) and its receptor TrkB.RESULTS WAS led to mucosal inflammation, visceral hyperalgesia, and high intestinal motility. Oral administration of BBR inhibited the NF-κB signal transduction pathway, reduced the expression of pro-inflammatory cytokines [interleukin (IL)- 1β, IL-6, interferon-γ, and tumor necrosis factor-α], promoted the expression of anti-inflammatory cytokines (IL-10 and transforming growth factor-β), and improved the terminal ileum tissue inflammation. BBR inhibited the expression of BDNF, TrkB, and C-kit in IBS rats, leading to the reduction of intestinal motility and visceral hypersensitivity. The therapeutic effect of BBR at a high dose (100 mg/kg) was superior to than that of the low-dose (25 mg/kg) group. CONCLUSION BBR reduces intestinal mucosal inflammation by inhibiting the intestinal NF-κB signal pathway in the IBS rats. BBR reduces the 展开更多
关键词 Irritable bowel syndrome Visceral hypersensitivity BERBERINE RIFAMPICIN Nuclear FACTOR KAPPA-B BRAIN-DERIVED neurotrophic FACTOR CAJAL mesenchymal cells Ckit
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Safety and efficacy of EFGR and VEGF signaling pathway inhibition therapy in patients with colorectal cancer: a meta-analysis 预览
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作者 Siwen Liu Rong Ma +7 位作者 Haixia Cao Shaorong Yu Dan Chen Changwen Jing Zhuo Wang Junying Zhang Jifeng Feng Jianzhong Wu 《肿瘤学与转化医学:英文版》 2019年第2期80-90,共11页
Objective Epidermal growth factor receptor(EGFR) and vascular endothelial growth factor(VEGF) inhibitors are two targeted therapies for metastatic colorectal cancer(mCRC). However, few studies have focused on the safe... Objective Epidermal growth factor receptor(EGFR) and vascular endothelial growth factor(VEGF) inhibitors are two targeted therapies for metastatic colorectal cancer(mCRC). However, few studies have focused on the safety and efficacy of combined targeted therapy against those of a single inhibition therapy of EFGR or VEGF. This meta-analysis aimed to compare the anti-tumor activity of the combined inhibition therapy and single inhibition therapy in patients with mCRC. Methods We searched PubMed, Medline, the Cochrane library, Embase, and annual meeting proceedings for relevant clinical trials. Objective response rate(ORR), progression-free survival(PFS), overall survival(OS), and adverse events were extracted and calculated.Results Nine trials comprising 3977 patients were selected for the analysis. The combined inhibition therapy showed a 3.7% improvement in ORR compared with single inhibition, and this difference was statistically significant [hazard ratio(HR)= 1.33;95% confidence interval(CI), 1.01–1.74;P = 0.04]. Subgroup analysis showed that the combined EGFR and VEGF inhibitor therapy had an 11.65% improvement in ORR compared with VEGF inhibitor therapy(OR = 2.14;95% CI, 1.34–3.40;P = 0.001). EGFR and VEGF inhibitor therapy and chemotherapy had an 18.08% improvement in ORR compared with chemotherapy(OR = 2.21;95% CI, 1.05–4.64;P = 0.04). Moreover, EGFR and VEGF inhibitor therapy significantly improved PFS compared with VEGF inhibitor therapy(OR = 0.82;95% CI, 0.69–0.97;P = 0.02). VEGF inhibitor therapy and chemotherapy significantly improved PFS compared with EGFR and VEGF inhibitor therapy and chemotherapy(OR = 1.20;95% CI, 1.11–1.30;P = 0.00). In addition, EGFR and VEGF inhibitor therapy showed improved OS compared with VEGF inhibitor therapy(HR = 0.78, 95% CI: 0.65–0.94;P = 0.008). Finally, the combined inhibition therapy showed an obviously increased risk of cutaneous and mucosal effects(RR = 6.45;95% CI: 2.71–15.36;P < 0.01), diarrhea/abdominal pain(RR = 1.97;95% CI: 1.45–2.68;P < 0.01), 展开更多
关键词 colorectal cancer (CRC) EPIDERMAL GROWTH FACTOR receptor (EGFR) vascular endothelial GROWTH FACTOR (VEGF) META-ANALYSIS
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Hyperbaric oxygen improves functional recovery of rats after spinal cord injury via activating stromal cell-derived factor-1/CXC chemokine receptor 4 axis and promoting brain-derived neurothrophic factor expression
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作者 Xiang-Long Meng Yong Hai +6 位作者 Xi-Nuo Zhang Yun-Sheng Wang Xue-Hua Liu Lin-Lin Ma Rong Yue Gang Xu Zhuo Li 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第6期699-706,共8页
Background: Spinal cord injury (SCI) is a worldwide medical concern. This study aimed to elucidate the mechanism underlying protective effect of hyperbaric oxygen (HBO) against SCI-induced neurologic defects in rats v... Background: Spinal cord injury (SCI) is a worldwide medical concern. This study aimed to elucidate the mechanism underlying protective effect of hyperbaric oxygen (HBO) against SCI-induced neurologic defects in rats via exploring the stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis and expression of brain-derived neurotrophic factor (BDNF). Methods: An acute SCI rat model was established in Sprague-Dawley rats using the Allen method. Sixty rats were divided into four groups (w = 15 in each group): sham-operated, SCI, SCI treated with HBO (SCI + HBO), and SCI treated with both HBO and AMD3100 (an antagonist of CXCR4;SCI + HBO + AMD) groups. The rats were treated with HBO twice a day for 3 days and thereafter once a day after the surgery for up to 28 days. Following the surgery, neurologic assessments were performed with the Basso-Bettie-Bresnahan (BBB) scoring system on postoperative day (POD) 7, 14, 21, and 28. Spinal cord tissues were harvested to assess the expression of SDF-1, CXCR4, and BDNF at mRNA and protein levels, using quantitative real-time polymerase chain reaction, Western blot analysis, and histopathologic analysis. Results: HBO treatment recovered SCI-induced descent of BBB scores on POD 14,(1.25±0.75 vs. 1.03 ±0.66, P< 0.05), 21 (5.27± 0.89 vs. 2.56± 1.24, P< 0.05), and 28 (11.35±0.56 vs. 4.23± 1.20, P<0.05) compared with the SCI group. Significant differences were found in the mRNA levels of SDF-1 (mRNA: day 21, SCI + HBO vs. SCI + HBO + AMD, 2.89± 1.60 vs. 1.56±0.98, P<0.05), CXCR4 (mRNA: day 7, SCI + HBO vs. SCI, 2.99± 1.60 vs. 1.31 ±0.98, P<0.05;day 14, SCI + HBO vs. SCI + HBO + AMD, 4.18± 1.60 vs. 0.80±0.34, P<0.05;day 21, SCI + HBO vs. SCI, 2.10±1.01 vs.1.15±0.03, P<0.05), and BDNF (mRNA: day 7, SCI + HBO vs. SCI, 3.04±0.41 vs. 2.75±0.31, P<0.05;day 14, SCI + HBO vs. SCI, 3.88± 1.59 vs. 1.11 ±0.40, P<0.05), indicating the involvement of SDF-1/CXCR4 axis in the protective effect of HBO. Conclusions: HBO might promote the recovery of neurologic func 展开更多
关键词 BRAIN-DERIVED NEUROTROPHIC factor CXC CHEMOKINE receptor 4 HYPERBARIC oxygen NEUROTROPHIC STROMAL cell-derived factor-1 Spinal cord injury
Role of hepatocyte nuclear factor 4-alpha in gastrointestinal and liver diseases 预览
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作者 Matthew M Yeh Dustin E Bosch Sayed S Daoud 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第30期4074-4091,共18页
Hepatocyte nuclear factor 4-alpha(HNF4α)is a highly conserved member of nuclear receptor superfamily of ligand-dependent transcription factors that is expressed in liver and gastrointestinal organs(pancreas,stomach,a... Hepatocyte nuclear factor 4-alpha(HNF4α)is a highly conserved member of nuclear receptor superfamily of ligand-dependent transcription factors that is expressed in liver and gastrointestinal organs(pancreas,stomach,and intestine).In liver,HNF4αis best known for its role as a master regulator of liver-specific gene expression and essential for adult and fetal liver function.Dysregulation of HNF4αexpression has been associated with many human diseases such as ulcerative colitis,colon cancer,maturity-onset diabetes of the young,liver cirrhosis,and hepatocellular carcinoma.However,the precise role of HNF4αin the etiology of these human pathogenesis is not well understood.Limited information is known about the role of HNF4αisoforms in liver and gastrointestinal disease progression.There is,therefore,a critical need to know how disruption of the expression of these isoforms may impact on disease progression and phenotypes.In this review,we will update our current understanding on the role of HNF4αin human liver and gastrointestinal diseases.We further provide additional information on possible use of HNF4αas a target for potential therapeutic approaches. 展开更多
关键词 HEPATOCYTE nuclear FACTOR 4-alpha Liver cirrhosis Hepatocellular CARCINOMA Viral hepatitis Gastrointestinal TRACT Colorectal CARCINOMA Transcription FACTOR
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Effect of laparoscopic hepatectomy on growth factor, immunoglobulin and related factors in patients with hepatocellular carcinoma
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作者 Gang Wang Gen-Hai Shen +2 位作者 Quan-Gen Gao Jian-Mao Yuan Li-Qiang Qian 《海南医科大学学报(英文版)》 2019年第12期53-56,共4页
Objective:To compare the effects of laparoscopic surgery and traditional open surgery on serum growth factor, immunoglobulin and related factors in patients with hepatocellular carcinoma after operation.Methods: 60 pa... Objective:To compare the effects of laparoscopic surgery and traditional open surgery on serum growth factor, immunoglobulin and related factors in patients with hepatocellular carcinoma after operation.Methods: 60 patients with hepatocellular carcinoma admitted to our hospital from 2014 to 2018 were randomly divided into the control group and the observation group, with 30 cases in each group. Patients in the control group underwent traditional open radical hepatectomy, while patients in the observation group underwent laparoscopic radical hepatectomy. Before and after the operation, the levels of Alpha-alanine aminotransferase (ALT), aspartate aminotransferase (AST), vascular endothelial growth factor (VEGF), acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF), immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin G (IgG), matrix metalloproteinase-13 (MMP-13), Procalcitonin (PCT) and C-reactive protein (CRP) were compared between the patients in the two groups.Results:The levels of ALT, AST, VEGF, aFGF, bFGF, IgA, IgM, and IgG of patients in the control group were significantly decreased after the operation, but the levels of MMP-13, PCT and CRP increased significantly. The serum levels of ALT, AST, VEGF, aFGF, bFGF, IgA, IgM, and IgG of patients in the observation group were significantly decreased after operation, but the levels of MMP-13, PCT and CRP increased significantly. The above indexes of patients in the observation group were better than those in the control group (P<0.05).Conclusion: Laparoscopic hepatectomy has a good clinical effect. It can significantly reduce the level of growth factor in serum of patients. It has less damage to immune function than open surgery and can reduce the level of inflammatory stress caused by operation. 展开更多
关键词 LAPAROSCOPIC SURGERY Open SURGERY Liver cancer Growth FACTOR IMMUNOGLOBULIN Inflammatory FACTOR
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