期刊文献+
共找到226篇文章
< 1 2 12 >
每页显示 20 50 100
Effects of miR-219/miR-338 on microglia and astrocyte behaviors and astrocyte-oligodendrocyte precursor cell interactions 预览
1
作者 Lan Huong Nguyen William Ong +3 位作者 Kai Wang Mingfeng Wang Dean Nizetic Sing Yian Chew 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期739-747,共9页
MiR-219 and miR-338(miR-219/miR-338)are oligodendrocyte-specific microRNAs.The overexpression of these miRs in oligodendrocyte precursor cells promotes their differentiation and maturation into oligodendrocytes,which ... MiR-219 and miR-338(miR-219/miR-338)are oligodendrocyte-specific microRNAs.The overexpression of these miRs in oligodendrocyte precursor cells promotes their differentiation and maturation into oligodendrocytes,which may enhance axonal remyelination after nerve injuries in the central nervous system(CNS).As such,the delivery of miR-219/miR-338 to the CNS to promote oligodendrocyte precursor cell differentiation,maturation and myelination could be a promising approach for nerve repair.However,nerve injuries in the CNS also involve other cell types,such as microglia and astrocytes.Herein,we investigated the effects of miR-219/miR-338 treatment on microglia and astrocytes in vitro and in vivo.We found that miR-219/miR-338 diminished microglial expression of pro-inflammatory cytokines and suppressed astrocyte activation.In addition,we showed that miR-219/miR-338 enhanced oligodendrocyte precursor cell differentiation and maturation in a scratch assay paradigm that re-created a nerve injury condition in vitro.Collectively,our results suggest miR-219/miR-338 as a promising treatment for axonal remyelination in the CNS following nerve injuries.All experimental procedures were approved by the Institutional Animal Care and Use Committee(IACUC),Nanyang Technological University(approval No.A0309 and A0333)on April 27,2016 and October 8,2016. 展开更多
关键词 central nervous system electrospinning gene SILENCING GLIA hydrogel MYELINATION nanofibers oligodendroglial POLYCAPROLACTONE spinal cord injury
在线阅读 下载PDF
Genetic targeting of astrocytes to combat neurodegenerative disease 预览
2
作者 Rachel Kéry Allen P. F. Chen Gregory W. Kirschen 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期199-211,共13页
Astrocytes, glial cells that interact extensively with neurons and other support cells throughout the central nervous system, have recently come under the spotlight for their potential contribution to, or potential re... Astrocytes, glial cells that interact extensively with neurons and other support cells throughout the central nervous system, have recently come under the spotlight for their potential contribution to, or potential regenerative role in a host of neurodegenerative disorders. It is becoming increasingly clear that astrocytes, in concert with microglial cells, activate intrinsic immunological pathways in the setting of neurodegenerative injury, although the direct and indirect consequences of such activation are still largely unknown. We review the current literature on the astrocyte’s role in several neurodegenerative diseases, as well as highlighting recent advances in genetic manipulation of astrocytes that may prove critical to modulating their response to neurological injury, potentially combatting neurodegenerative damage. 展开更多
关键词 Alzheimer's DISEASE amyotrophic lateral SCLEROSIS GLIA immune system inflammation Parkinson's DISEASE reactive ASTROCYTE regeneration
在线阅读 下载PDF
Adult neurogenesis from reprogrammed astrocytes 预览
3
作者 Brian B.Griffiths Anvee Bhutani Creed MStary 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第6期973-979,共7页
The details of adult neurogenesis,including environmental triggers,region specificity,and species homology remain an area of intense investigation.Slowing or halting age-related cognitive dysfunction,or restoring neur... The details of adult neurogenesis,including environmental triggers,region specificity,and species homology remain an area of intense investigation.Slowing or halting age-related cognitive dysfunction,or restoring neurons lost to disease or injury represent just a fraction of potential therapeutic applications.New neurons can derive from stem cells,pluripotent neural progenitor cells,or non-neuronal glial cells,such as astrocytes.Astrocytes must be epigenetically"reprogrammed"to become neurons,which can occur both naturally in vivo,and via artificial exogenous treatments.While neural progenitor cells are localized to a few neurogenic zones in the adult brain,astrocytes populate almost every brain structure.In this review,we will summarize recent research into neurogenesis that arises from conversion of post-mitotic astrocytes,detail the genetic and epigenetic pathways that regulate this process,and discuss the possible clinical relevance in supplementing stem-cell neurogenic therapies. 展开更多
关键词 ASTROCYTE brain DEDIFFERENTIATION development disease GLIA INJURY NEUROGENESIS
在线阅读 下载PDF
Adrenomedullin:an important participant in neurological diseases 预览
4
作者 Feng-Jiao Li Si-Ru Zheng Dong-Mei Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第7期1199-1207,共9页
Adrenomedullin,a peptide with multiple physiological functions in nervous system injury and disease,has aroused the interest of researchers.This review summarizes the role of adrenomedullin in neuropathological disord... Adrenomedullin,a peptide with multiple physiological functions in nervous system injury and disease,has aroused the interest of researchers.This review summarizes the role of adrenomedullin in neuropathological disorders,including pathological pain,brain injury and nerve regeneration,and their treatment.As a newly characterized pronociceptive mediator,adrenomedullin has been shown to act as an upstream factor in the transmission of noxious information for various types of pathological pain including acute and chronic inflammatory pain,cancer pain,neuropathic pain induced by spinal nerve injury and diabetic neuropathy.Initiation of glia-neuron signaling networks in the peripheral and central nervous system by adrenomedullin is involved in the formation and maintenance of morphine tolerance.Adrenomedullin has been shown to exert a facilitated or neuroprotective effect against brain injury including hemorrhagic or ischemic stroke and traumatic brain injury.Additionally,adrenomedullin can serve as a regulator to promote nerve regeneration in pathological conditions.Therefore,adrenomedullin is an important participant in nervous system diseases. 展开更多
关键词 ADRENOMEDULLIN brain injury GLIA mechanism morphine tolerance neural regeneration neuroprotective effect pathological pain REGENERATION SENSITIZATION TARGET
在线阅读 下载PDF
Different modes of foveal regeneration after closure of full-thickness macular holes by(re)vitrectomy and autologous platelet concentrate
5
作者 Andreas Bringmann Claudia Jochmann +3 位作者 Jan Darius Unterlauft Renate Wiedemann Matus Rehak Peter Wiedemann 《国际眼科杂志:英文版》 SCIE CAS 2020年第1期36-48,共13页
AIM: To describe using spectral-domain optical coherence tomography the regeneration of the foveal morphology after pars plana(re)vitrectomy surgery and gas tamponade combined with injection of autologous platelet con... AIM: To describe using spectral-domain optical coherence tomography the regeneration of the foveal morphology after pars plana(re)vitrectomy surgery and gas tamponade combined with injection of autologous platelet concentrate to treat full-thickness macular holes, and to describe different anatomical outcome. METHODS: A retrospective case series of 8 eyes of 8 patients was described. RESULTS: In all cases investigated, the plateletassisted closure of macular holes was associated with a rapid resolution of cystic cavities in the foveal walls. In two patients, there was a regular regeneration of the foveal morphology after hole closure;the regenerated central fovea had a regular structure with a foveola and photoreceptors. In three other patients, there was an irregular regeneration of the fovea;a foveola was not formed, photoreceptor cells were absent from the foveal center, and the center was composed of Müller and retinal pigment epithelial(RPE) cells. The foveal regeneration after hole closure may proceed with or without a temporary detachment of the foveal center from the RPE, and with or without a direct contact between the central outer nuclear layer(ONL) and the RPE. Contacts between the ONL and RPE were observed only in patients with an irregular foveal regeneration after hole closure.CONCLUSION: The data show that there are different modes of foveal regeneration after closure of macular holes with(re)vitrectomy and platelet concentrate. It is suggested that the regular regeneration of the foveal morphology proceeds by Müller cell-mediated tissue movements without cell proliferation, whereas the irregular foveal regeneration proceeds in part by proliferation of Müller and RPE cells. 展开更多
关键词 macular hole platelet concentrate FOVEA Müller glia retinal pigment epithelium
Rapid transport of insulin to the brain following intranasal administration in rats 预览
6
作者 Lir-Wan Fan Kathleen Carter +1 位作者 Abhay Bhatt Yi Pang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期1046-1051,共6页
We previously reported that intranasal insulin protects substantia nigra dopaminergic neurons against 6-hydroxydopamine neurotoxicity in rats.This study aimed to assess insulin pharmacokinetics in the rat brain follow... We previously reported that intranasal insulin protects substantia nigra dopaminergic neurons against 6-hydroxydopamine neurotoxicity in rats.This study aimed to assess insulin pharmacokinetics in the rat brain following intranasal application.Recombinant human insulin(rh-Ins)or phosphate buffer solution was administered to both nostrils of rats.Animals were sacrificed at 15 minutes,1,2,and 6 hours to determine insulin levels in different brain regions by an ultrasensitive,human-specific enzyme-linked immunosorbent assay kit.For fluorescence tracing study,rats were administered with intranasal florescence-tagged insulin(Alex546-Ins),and brains were fixed at 10 and 30 minutes to prepare sagittal sections.rh-Ins was detected in all brain regions examined except the cerebral cortex.The highest levels were detected in the brainstem,followed by the cerebellum,substantia nigra/ventral tegmental area,olfactory bulb,striatum,hippocampus,and thalamus/hypothalamus.Insulin levels reached a peak at 15 minutes and then declined gradually overtime,but remained significantly higher than baseline levels at 6 hours in most regions.Consistently,widespread Alex546-Ins-binding cells were detected in the brain at 10 and 30 minutes,with the olfactory bulb and brainstem showing the highest while the cerebral cortex showing lowest fluorescence signals.Double-immunostaining showed that Alex546-Ins-bindings were primarily co-localized with neuronal nuclei-positive neurons.In the subtantia nigra,phospho-Akt was found to be activated in a subset of Alex546-Ins and tyrosine hydroxylase double-labeled cells,suggesting activation of the Akt/PI3K pathway in these dopaminergic neurons.Data from this study suggest that intranasal insulin could effectively reach deep brain structures including the nigrostriatal pathways,where it binds to dopaminergic neurons and activates intracellular cell survival signaling.This study was approved by the Institutional Animal Care Committee at the University of Mississippi Medical Center(protocol 1333A)on June 29 展开更多
关键词 dopaminergic neurons STRIATUM substantia nigra BRAINSTEM olfactory bulb GLIA TRIGEMINAL nerve pharmacokinetics axonal TRANSPORT PAKT
在线阅读 下载PDF
α-突触核蛋白在大鼠脊髓损伤后的表达及意义 预览
7
作者 钟占琼 薛清彩 +3 位作者 刘晓芬 许明珠 向阳 张晓 《中国医药导刊》 2019年第3期170-176,共7页
目的:探究大鼠脊髓损伤(spinal cord injury,SCI)后α-突触核蛋白(α-synuclein, SNCA)在脊髓中的表达和意义。方法:SD大鼠随机分为对照组和SCI组,采用Allen′s法制备大鼠SCI模型。根据基因芯片分析结果,筛选出目的基因。应用实时定量PC... 目的:探究大鼠脊髓损伤(spinal cord injury,SCI)后α-突触核蛋白(α-synuclein, SNCA)在脊髓中的表达和意义。方法:SD大鼠随机分为对照组和SCI组,采用Allen′s法制备大鼠SCI模型。根据基因芯片分析结果,筛选出目的基因。应用实时定量PCR和免疫组织化学方法分别检测SNCA在脊髓中的定位表达和变化。生物信息学分析预测SNCA相关基因或蛋白。结果:基因芯片结果显示差异下调基因SNCA差异表达倍数2.276,验证结果显示SNCA下调。实时定量PCR显示SNCA的 mRNA在大鼠SCI后3、7、14和28 d的表达量明显降低,与对照组相比,差异均有统计学意义( P <0.001);免疫组织化学结果显示SNCA在正常脊髓后角的神经元突起、神经胶质细胞突起以及前角神经元胞浆中表达,在SCI后7 d和14 d,与对照组相比,SNCA免疫阳性物质明显减少。生物信息学分析结果显示SNCA与神经递质传递、氧化应激和细胞凋亡等有关。结论:SCI后SNCA的mRNA和蛋白表达水平均降低,在脊髓前角和后角的表达明显减少。提示在SCI后SNCA可能参与调控神经元和神经胶质细胞的存活,进一步影响SCI后的运动功能和感觉功能。 展开更多
关键词 脊髓损伤 Α-突触核蛋白 神经元 神经胶质细胞
在线阅读 免费下载
Nogo-A蛋白参与癫痫发生机制的研究进展
8
作者 章津伟 陈涛 +2 位作者 余思逊 树海峰 匡永勤 《中华神经科杂志》 CAS CSCD 北大核心 2019年第11期967-973,共7页
髓鞘相关蛋白Nogo-A最早被认为是轴突生长抑制因子,参与神经系统多种病理生理调节。近年来越来越多的研究表明,Nogo-A蛋白通过参与调控树突可塑性、介导神经异常迁移、调节胶质细胞活化等与癫痫的发生有着密切的关系。文中将对近年来Nog... 髓鞘相关蛋白Nogo-A最早被认为是轴突生长抑制因子,参与神经系统多种病理生理调节。近年来越来越多的研究表明,Nogo-A蛋白通过参与调控树突可塑性、介导神经异常迁移、调节胶质细胞活化等与癫痫的发生有着密切的关系。文中将对近年来Nogo-A在癫痫发生中的联系研究进展做一综述。 展开更多
关键词 NOGO-A 癫痫 树突 胶质细胞 神经迁移
The neuro-glial coagulonome: the thrombin receptor and coagulation pathways as major players in neurological diseases 预览
9
作者 Shany G. Gofrit Efrat Shavit-Stein 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2043-2053,共11页
The neuro-glial interface extends far beyond mechanical support alone and includes interactions through coagulation cascade proteins. Here, we systematically review the evidence indicating that synaptic and node of Ra... The neuro-glial interface extends far beyond mechanical support alone and includes interactions through coagulation cascade proteins. Here, we systematically review the evidence indicating that synaptic and node of Ranvier glia cell components modulate synaptic transmission and axonal conduction by a coagulation cascade protein system, leading us to propose the concept of the neuro-glial coagulonome. In the peripheral nervous system, the main thrombin receptor protease activated receptor 1 (PAR1) is located on the Schwann microvilli at the node of Ranvier and at the neuromuscular junction. PAR1 activation effects can be both neuroprotective or harmful, depending on thrombin activity levels. Low physiological levels of thrombin induce neuroprotective effects in the Schwann cells which are mediated by the endothelial protein C receptor. High levels of thrombin induce conduction deficits, as found in experimental autoimmune neuritis, the animal model for Guillaine-Barre syndrome. In the central nervous system, PAR1 is located on the peri-synaptic astrocyte end-feet. Its activation by high thrombin levels is involved in the pathology of primary inflammatory brain diseases such as multiple sclerosis, as well as in other central nervous system insults, including trauma, neoplasms, epilepsy and vascular injury. Following activation of PAR1 by high thrombin levels the seizure threshold is lowered. On the other hand, PAR1 activation by lower levels of thrombin in the central nervous system protects against a future ischemic insult. This review presents the known structure and function of the neuro-glial coagulonome, focusing on coagulation, thrombin and PAR1 in a pathway which may be either physiological (neuroprotective) or detrimental in peripheral nervous system and central nervous system diseases. Understanding the neuro-glial coagulonome may open opportunities for novel pharmacological interventions in neurological diseases. 展开更多
关键词 THROMBIN PROTEASE activated receptor 1 PROTEASE nexin 1 GLIA node of Ranvier SYNAPSE epilepsy GLIOBLASTOMA Guillaine-Barre syndrome
在线阅读 下载PDF
Effects of LW-AFC on anxiety-like behavior and immune function in corticosterone-induced mice 预览
10
作者 ZENG Ju CHENG Xiao-rui +1 位作者 ZHOU Wen-xia ZHANG Yong-xiang 《中国药理学与毒理学杂志》 CAS 北大核心 2019年第9期678-679,共2页
OBJECTIVE Chronic stress is one of the important factors in the development of many mental and neurological diseases,and cause damage to the central nervous system,affect animal emotions and damage the immune function... OBJECTIVE Chronic stress is one of the important factors in the development of many mental and neurological diseases,and cause damage to the central nervous system,affect animal emotions and damage the immune function of the body.The purpose of this study was to investigate the effects of LW-AFC which extracting from traditional Chinese medicine prescription Liuwei Dihuang decoctionon the anxiety-like behaviorand immune dysfunction abnormalities caused by chronic stress,and whether immune intervention affect the action of LW-AFC.METHODS Male BALB/c mice were subcutaneously injected with corticosterone(25 mg·kg^-1)for 28 d to establish a chronic stress model.Cyclophos⁃phamide(Cy,80 mg·kg^-1)was injected continuously for the initial three days,followed by once a week,LW-AFC(1.6 g·kg^-1)was given continuously for 28 d.Then investigate the emotion changes by open field and elevated plus maze tests,and detected the lymphocyte proliferation,lymphocyte subsets in peripheral blood,microglia and astrocyte expression,and inflammatory cytokines in peripheral blood and brain tissue.RESULTS The mice showed obvious depressive-like behaviorafter 28 d of continuous corticosterone injection.LW-AFC could significantly improve the anxietybehavior induced by corticosterone injection,but LW-AFC could not improve the anxietybehavior of mice by Cy intervention.The expression of glial cells in hippocampus of corticosterone-induced mice showed an upward trend,and the activation of microglia and astrocytes have significantly increase in corticosterone and Cy injected mice.LW-AFC significantly decreased the activation of microglia and astrocytes in corticosterone-induced mice with Cy intervention.This suggested that LW-AFC can reduce the damage of stress on the immune function of central nervous system under immunosuppres⁃sive state.Furthermore,LW-AFC could significantly up-regulate the proliferation of splenic lymphocyte stimulated by LPS and ConA,up-regulate the proportion of CD3+CD8+cells,reduce the proportion of CD4+/CD8+cells,d 展开更多
关键词 LW-AFC CORTICOSTERONE CYCLOPHOSPHAMIDE anxiety-like behavior glia cells LYMPHOCYTE inflammatory factors
在线阅读 免费下载
Attenuation of periostin in retinal Müller glia by TNF-αand IFN-γ
11
作者 Ying-Qian Peng Man-Jing Cao +8 位作者 Shigeo Yoshida Lu-Si Zhang Hui-Lan Zeng Jing-Ling Zou Yoshiyuki Kobayashi Takahito Nakama Jing-Ming Shi Song-Bai Jia Ye-Di Zhou 《国际眼科杂志:英文版》 SCIE CAS 2019年第2期212-218,共7页
AIM:To investigate the regulation and mechanisms of periostin expression in retinal Müller glia, and to explore the relevance to retinal neovascularization. METHODS:The oxygen-induced retinopathy(OIR) mouse model... AIM:To investigate the regulation and mechanisms of periostin expression in retinal Müller glia, and to explore the relevance to retinal neovascularization. METHODS:The oxygen-induced retinopathy(OIR) mouse model and the human Moorfield/Institute of Ophthalmology-Müller 1(MIO-M1) cell line were used in the study. Immunofluorescence staining was used to determine the distribution and expression of periostin and a Müller glial cell marker glutamine synthetase(GS). Cytokines TNF-α and IFN-γ were added to stimulate the MIO-M1 cells. ShRNA was used to knockdown periostin expression in MIO-M1 cells. Quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR) was conducted to assess the mRNA expression of periostin. RESULTS:Immunofluorescence staining showed that periostin was expressed by MIO-M1 Müller glia. GS-positive Müller glia and periostin increased in OIR retinas, and were partially overlaid. The stimulation of TNF-α and IFN-γ reduced the mRNA expression of periostin significantly and dose-dependently in MIO-M1 cells. Knockdown of periostin reduced mRNA expression of vascular endothelial growth factor A(VEGFA) in MIO-M1 cells, while VEGFA expression was not changed in periostin knock-out OIR retinas. CONCLUSION:Müller glia could be one of the main sources of periostin in the retina, and might contribute to the pathogenesis of retinal neovascularization. Proinflammatory cytokines TNF-α and IFN-γ attenuate the periostin expression in retinal Müller glia, which provides a potential and novel method in treating retinal neovascular diseases. 展开更多
关键词 TNF-Α IFN-Γ PERIOSTIN Müller GLIA RETINAL neovascularization
H3K27me3 Signal in the Cis Regulatory Elements Reveals the Differentiation Potential of Progenitors During Drosophila Neuroglial Development
12
作者 Xiaolong Chen Youqiong Ye +6 位作者 Liang Gu Jin Sun Yanhua Du Wen-Ju Liu Wei Li Xiaobai Zhang Cizhong Jiang 《基因组蛋白质组与生物信息学报:英文版》 CAS CSCD 2019年第3期297-304,共8页
Drosophila neural development undergoes extensive chromatin remodeling and precise epigenetic regulation.However,the roles of chromatin remodeling in establishment and maintenance of cell identity during cell fate tra... Drosophila neural development undergoes extensive chromatin remodeling and precise epigenetic regulation.However,the roles of chromatin remodeling in establishment and maintenance of cell identity during cell fate transition remain enigmatic.Here,we compared the changes in gene expression,as well as the dynamics of nucleosome positioning and key histone modifications between the four major neural cell types during Drosophila neural development.We find that the neural progenitors can be separated from the terminally differentiated cells based on their gene expression profiles,whereas nucleosome distribution in the flanking regions of transcription start sites fails to identify the relationships between the progenitors and the differentiated cells.H3K27me3 signal in promoters and enhancers can not only distinguish the progenitors from the differentiated cells but also identify the differentiation path of the neural stem cells(NSCs)to the intermediate progenitor cells to the glial cells.In contrast,H3K9ac signal fails to identify the differentiation path,although it activates distinct sets of genes with neuron-specific and glia-related functions during the differentiation of the NSCs into neurons and glia,respectively.Together,our study provides novel insights into the crucial roles of chromatin remodeling in determining cell type during Drosophila neural development. 展开更多
关键词 NUCLEOSOME HISTONE modification Neural stem cell NEURON GLIA
Glutamate transporters,EAAT1 and EAAT2,are potentially important in the pathophysiology and treatment of schizophrenia and affective disorders 预览
13
作者 Georgia M Parkin Madhara Udawela +1 位作者 Andrew Gibbons Brian Dean 《世界精神病学杂志》 2018年第2期51-63,共13页
Glutamate is the predominant excitatory neurotransmitter in the human brain and it has been shown that prolonged activation of the glutamatergic system leads to nerve damage and cell death.Following release from the p... Glutamate is the predominant excitatory neurotransmitter in the human brain and it has been shown that prolonged activation of the glutamatergic system leads to nerve damage and cell death.Following release from the pre-synaptic neuron and synaptic transmission,glutamate is either taken up into the presynaptic neuron or neighbouring glia by transmembrane glutamate transporters.Excitatory amino acid transporter(EAAT)1 and EAAT2 are Na+-dependant glutamate transporters expressed predominantly in glia cells of the central nervous system.As the most abundant glutamate transporters,their primary role is to modulate levels of glutamatergic excitability and prevent spill over of glutamate beyond the synapse.This role is facilitated through the binding and transportation of glutamate into astrocytes and microglia.The function of EAAT1 and EAAT2 is heavily regulated at the levels of gene expression,post-transcriptional splicing,glycosylation states and cell-surface trafficking of the protein.Both glutamatergic dysfunction and glial dysfunction have been proposed to be involved in psychiatric disorder.This review will present an overview of the roles that EAAT1 and EAAT2 play in modulating glutamatergic activity in the human brain,and mount an argument that these two transporters could be involved in the aetiologies of schizophrenia and affective disorders as well as represent potential drug targets for novel therapies for those disorders. 展开更多
关键词 GLIA EXCITATORY amino acid TRANSPORTER PSYCHIATRY Affective disorders GLUTAMATE TRANSPORTER GLUTAMATE SCHIZOPHRENIA
在线阅读 免费下载
鞘内注射SOCS3对神经病理性疼痛模型小鼠痛行为及脊髓促炎因子表达的影响
14
作者 张婷 孙凯 +3 位作者 张法强 祁乐 申文 王立伟 《中华行为医学与脑科学杂志》 CSCD 北大核心 2018年第10期865-869,共5页
目的探讨脊髓水平细胞因子信号传导抑制蛋白3(suppressor of cytokine signaling 3,SOCS3)在小鼠神经病理性疼痛中的作用。方法第一部分:雄性昆明小鼠4只,采用坐骨神经结扎方法制备小鼠神经病理性疼痛(chronic sciatic constricti... 目的探讨脊髓水平细胞因子信号传导抑制蛋白3(suppressor of cytokine signaling 3,SOCS3)在小鼠神经病理性疼痛中的作用。方法第一部分:雄性昆明小鼠4只,采用坐骨神经结扎方法制备小鼠神经病理性疼痛(chronic sciatic constriction injury,CCI)模型,术后7d处死小鼠取脊髓L4~6腰膨大,采用免疫荧光技术检测CCI小鼠脊髓背角SOCS3分别与神经元标记物(NeuN)、星形胶质细胞标记物(GFAP)、小胶质细胞标记物(IBA1)的共表达。第二部分:雄性昆明小鼠32只,按照随机数字表法分为假手术组(SH组),神经病理性疼痛组(BP组),神经病理性疼痛+SOCS3过表达慢病毒(BS组),神经病理性疼痛+对照慢病毒组(BV组)。BS组和BV组于模型制备成功后7~9d分别鞘内注射SOCS3过表达慢病毒2μl、空载慢病毒2μl。分别于术前1d、术后5,7,10,12,14d,测定热缩足潜伏期(PWL)和机械缩足阈值(PWT),术后14d处死小鼠,取脊髓L4~6腰膨大节段,采用Western blot技术检测GFAP和IBA-1表达,采用Elisa法测定促炎因子IL-6,IL-1β,TNF-α的表达。结果SOCS3在神经病理性疼痛小鼠脊髓背角主要表达于星形胶质细胞和小胶质细胞,在神经元中几乎没有表达。与SH组比较,BP组、BV组术后PWL和PWT明显降低(P〈0.05),脊髓GFAP、IBA-1,炎症相关因子IL-6、IL-18、TNF·仪表达升高(P〈0.05);与BV组相比,BS组10、12、14天PWL[(5.1±0.9),(7.5±0.8),(7.2±1.4)]和PWT[(6.1±1.4),(8.9±1.1),(8.2±2.1)]明显升高(均P〈0.05),脊髓GFAP(1.69±0.45)、IBA-1(1.76±0.25),炎症相关因子IL-6(181±8)、IL-1β(151±7)、TNF-α(216±9)表达减少(均P〈0.05)。结论神经病理性疼痛小鼠脊髓SOCS3主要表达于星形胶质细胞和小胶质细胞,鞘内注射SOCS3过表达慢病毒可能通过抑制胶质细胞活化和促炎因子IL-6,IL-1 展开更多
关键词 细胞因子信号传导抑制蛋白3 神经病理性疼痛 胶质细胞 脊髓 小鼠
干扰素在中枢神经系统退行性疾病中的作用 预览
15
作者 向敏 舒扬 +4 位作者 汪毅 毕明达 汪雪峰 冯玎琦 毛佳慧 《中国组织化学与细胞化学杂志》 CAS CSCD 2018年第3期261-267,共7页
干扰素(Interferons,IFNs)在神经系统退行性疾病中作用机制复杂。IFNs受体在神经元与胶质细胞中广泛表达,提示IFNs在神经系统中发挥重要作用。然而,IFNs在中枢神经系统中的功能,特别是在相关疾病中的作用机制仍不清楚。近年来,越来越多... 干扰素(Interferons,IFNs)在神经系统退行性疾病中作用机制复杂。IFNs受体在神经元与胶质细胞中广泛表达,提示IFNs在神经系统中发挥重要作用。然而,IFNs在中枢神经系统中的功能,特别是在相关疾病中的作用机制仍不清楚。近年来,越来越多的研宄关注IFNs在中枢神经系统中的作用以及对神经元和胶质细胞的调控机制。本文主要就IFNs的结构、功能和疾病的关系进行综述。 展开更多
关键词 神经系统 干扰素 炎症 神经元 胶质细胞
在线阅读 下载PDF
脊髓背角胶质细胞及p38 MAPK参与介导髓核致炎大鼠神经根痛 预览
16
作者 王益敏 易增兴 +6 位作者 林世清 张嘉明 蔡哲 贺秋兰 魏明 孙来保 邹学农 《中国疼痛医学杂志》 CAS CSCD 北大核心 2018年第1期14-23,共10页
目的:探索脊髓背角胶质细胞及p38 MAPK在髓核致炎大鼠神经根痛中表达的变化。方法:雄性成年SD大鼠66只,随机分为3组:Blank组(12只)、Sham组(12只)、手术模型组(NP组,42只)。检测所有大鼠术前1天,术后1、3、7、12、14、21和28天... 目的:探索脊髓背角胶质细胞及p38 MAPK在髓核致炎大鼠神经根痛中表达的变化。方法:雄性成年SD大鼠66只,随机分为3组:Blank组(12只)、Sham组(12只)、手术模型组(NP组,42只)。检测所有大鼠术前1天,术后1、3、7、12、14、21和28天的50%机械性缩足阈值(50%MWT)。取各组大鼠术后1、3、7、14和28天术侧腰段脊髓背角,免疫荧光检测胶质细胞和p-p38表达与变化及p-p38与胶质细胞共表达情况。另一雄性成年SD大鼠84只,随机分为5组:Blank(12只),Sham(12只),NP(12只),DMSO(12只)和p38 MAPK抑制剂SB203580组(SB组,36只),DMSO组和SB组于术后第2天经硬膜外腔分别给予50μl 10%DMSO和0.1%SB203580,检测所有大鼠术前1天,术后1、2天及给药后1 h、3 h、5 h、7 h、12和24 h的50%MWT。Blank组,Sham组和NP组于术后第2天、DMSO组于给药后3 h取材,SB组分别于给药后3 h、7 h、12 h、24 h取材,Western blot检测p38和p-p38蛋白表达。结果:与Blank组相比,Sham组50%MWT仅在术后1 d下降(P〈0.05),NP组各时点的50%MWT明显降低(P〈0.05),可持续至术后28天。NP组胶质细胞和p-p38蛋白在术后不同时点发生表达(P〈0.05),免疫荧光双标显示p-p38大量表达于小胶质细胞。SB组在给药后3 h 50%MWT明显升高,持续到给药后12 h(P〈0.05),Western blot结果显示给药后3-12 h p-p38蛋白表达降低(P〈0.05)。结论:在髓核致炎神经根痛大鼠脊髓背角中出现胶质细胞激活及p-p38蛋白表达,给予p38 MAPK特异性抑制剂后痛敏发生改善,胶质细胞及p38 MAPK参与了髓核致炎大鼠神经根痛的发生和发展。 展开更多
关键词 脊髓背角 神经根炎性痛 胶质细胞 p38 MAPK 髓核
在线阅读 下载PDF
Axon degeneration: make the Schwann cell great again 预览
17
作者 Keit Men Wong Elisabetta Babetto Bogdan Beirowski 《中国神经再生研究:英文版》 SCIE CAS CSCD 2017年第4期518-524,共7页
Axonal degeneration is a pivotal feature of many neurodegenerative conditions and substantially accounts for neurological morbidity. A widely used experimental model to study the mechanisms of axonal degeneration is W... Axonal degeneration is a pivotal feature of many neurodegenerative conditions and substantially accounts for neurological morbidity. A widely used experimental model to study the mechanisms of axonal degeneration is Wallerian degeneration(WD), which occurs after acute axonal injury. In the peripheral nervous system(PNS), WD is characterized by swift dismantling and clearance of injured axons with their myelin sheaths. This is a prerequisite for successful axonal regeneration. In the central nervous system(CNS), WD is much slower, which significantly contributes to failed axonal regeneration. Although it is well-documented that Schwann cells(SCs) have a critical role in the regenerative potential of the PNS, to date we have only scarce knowledge as to how SCs ‘sense' axonal injury and immediately respond to it. In this regard, it remains unknown as to whether SCs play the role of a passive bystander or an active director during the execution of the highly orchestrated disintegration program of axons. Older reports, together with more recent studies, suggest that SCs mount dynamic injury responses minutes after axonal injury, long before axonal breakdown occurs. The swift SC response to axonal injury could play either a pro-degenerative role, or alternatively a supportive role, to the integrity of distressed axons that have not yet committed to degenerate. Indeed, supporting the latter concept, recent findings in a chronic PNS neurodegeneration model indicate that deactivation of a key molecule promoting SC injury responses exacerbates axonal loss. If this holds true in a broader spectrum of conditions, it may provide the grounds for the development of new glia-centric therapeutic approaches to counteract axonal loss. 展开更多
在线阅读 下载PDF
NRG1-ErbB2信号通路对骨癌痛大鼠脊髓胶质细胞及IL-1β的影响 预览 被引量:2
18
作者 蒋晶晶 姚鹏 +2 位作者 田悦 吴秀英 张锦 《实用药物与临床》 CAS 2016年第6期657-660,共4页
目的探讨NRG1-Erb B2信号通路对骨癌痛大鼠脊髓胶质细胞和IL-1β的影响。方法雌性SD大鼠,随机分为3组,每组12只。Sham组(假手术组);CIBP组:大鼠胫骨内注射Walker256乳腺癌细胞构建骨癌痛模型;CIBP+PD168393组:构建CIBP模型后6 d,... 目的探讨NRG1-Erb B2信号通路对骨癌痛大鼠脊髓胶质细胞和IL-1β的影响。方法雌性SD大鼠,随机分为3组,每组12只。Sham组(假手术组);CIBP组:大鼠胫骨内注射Walker256乳腺癌细胞构建骨癌痛模型;CIBP+PD168393组:构建CIBP模型后6 d,鞘内注入PD168393 10μg,每日1次,连续9 d,其余组鞘内注入生理盐水。接种瘤细胞后14 d,检测大鼠脊髓背角GFAP、OX42和IL-1β的变化。结果接种瘤细胞后14 d,CIBP组大鼠GFAP、OX42积分光密度值明显高于Sham组,差异有统计学意义(P〈0.01),肿瘤细胞的植入能诱导大鼠同侧脊髓背角GFAP和OX42的表达显著增加,并使星形胶质细胞和小胶质细胞的胞体肥大。而给予Erb B2受体抑制剂PD168393可明显抑制脊髓背角神经化学物质的改变,GFAP和OX42的表达均明显降低(P〈0.01)。CIBP组IL-1β于接种瘤细胞后14 d表达增加,明显高于Sham组(P〈0.01),给予PD168393能显著抑制IL-1β表达增加(P〈0.01)。结论大鼠胫骨接种瘤细胞后,脊髓背角内星形胶质细胞和小胶质细胞被广泛激活,IL-1β释放增加,阻断NRG1-Erb B2受体信号通路能有效抑制脊髓胶质细胞的表达和活化及炎症介质IL-1β的释放,从而产生镇痛作用。 展开更多
关键词 骨癌痛 NRG1 Erb B2 脊髓 胶质细胞 鞘内注射 痛觉过敏
在线阅读 下载PDF
Hippo通路在神经发育和神经系统疾病中的功能 被引量:2
19
作者 赵思奇 王树坤 +1 位作者 何晴 袁增强 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2016年第4期383-388,共6页
Hippo通路是一个调控组织器官大小、细胞增殖、分化和凋亡的高度保守的信号通路.我们研究了氧化压力条件下Hippo通路在神经细胞中的作用,并发现哺乳动物STE20样的丝-苏氨酸蛋白激酶(MST1)可参与氧化应激诱导的神经细胞凋亡,其上游受... Hippo通路是一个调控组织器官大小、细胞增殖、分化和凋亡的高度保守的信号通路.我们研究了氧化压力条件下Hippo通路在神经细胞中的作用,并发现哺乳动物STE20样的丝-苏氨酸蛋白激酶(MST1)可参与氧化应激诱导的神经细胞凋亡,其上游受非受体酪氨酸激酶c-Abl的调控.近期,我们研究发现MST1参与脑缺血引起的神经炎症,还发现Yes相关蛋白1(YAP)参与神经干细胞的自我更新.本文将介绍Hippo通路在中枢神经系统疾病和神经发育中的作用和机制研究的相关进展. 展开更多
关键词 MST1 YAP 神经元 神经干细胞 胶质细胞
Role of astrocytic glutamate transporter in alcohol use disorder 预览
20
作者 Jennifer R Ayers-Ringler Yun-Fang Jia +1 位作者 Yan-Yan Qiu Doo-Sup Choi 《世界精神病学杂志》 2016年第1期31-42,共12页
Alcohol use disorder(AUD)is one of the most widespread neuropsychiatric conditions,having a significant health and socioeconomic impact.According to the 2014 World Health Organization global status report on alcohol a... Alcohol use disorder(AUD)is one of the most widespread neuropsychiatric conditions,having a significant health and socioeconomic impact.According to the 2014 World Health Organization global status report on alcohol and health,the harmful use of alcohol is responsible for 5.9%of all deaths worldwide.Additionally,5.1%of the global burden of disease and injury is ascribed to alcohol(measured in disability adjusted life years,or disability adjusted life years).Although the neurobiological basis of AUD is highly complex,the corticostriatal circuit contributes significantly to the development of addictive behaviors.In-depth investigation into the changes of the neurotransmitters in this circuit,dopamine,gamma-aminobutyricacid,and glutamate,and their corresponding neuronal receptors in AUD and other addictions enable us to understand the molecular basis of AUD.However,these discoveries have also revealed a dearth of knowledge regarding contributions from nonneuronal sources.Astrocytes,though intimately involved in synaptic function,had until recently been noticeably overlooked in their potential role in AUD.One major function of the astrocyte is protecting neurons from excitotoxicity by removing glutamate from the synapse via excitatory amino acid transporter type 2.The importance of this key transporter in addiction,as well as ethanol withdrawal,has recently become evident,though its regulation is still under investigation.Historically,pharmacotherapy for AUD has been focused on altering the activity of neuronal glutamate receptors.However,recent clinical evidence has supported the animal-based findings,showing that regulating glutamate homeostasis contributes to successful management of recovery from AUD. 展开更多
关键词 Alcohol ADDICTION Glutamate ASTROCYTE EXCITATORY amino acid TRANSPORTER type 2 GLIA STRIATUM
在线阅读 免费下载
上一页 1 2 12 下一页 到第
使用帮助 返回顶部 意见反馈