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HPTLC-DPPH法定性定量分析保健品中芦丁 预览
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作者 王天楠 张煌 +2 位作者 陈益胜 徐学明 黄彩虹 《食品与机械》 北大核心 2019年第1期92-96,142共6页
将1,1-二苯基-2-三硝基苯肼(DPPH)还原变色反应与高效薄层色谱分离相结合,建立了一种HPTLC-DPPH联用测定保健品中芦丁的方法,并考察了DPPH显色反应时间、流动相配比、光密度扫描参数对色谱分离、扫描效果的影响,同时测试了该方法的回收... 将1,1-二苯基-2-三硝基苯肼(DPPH)还原变色反应与高效薄层色谱分离相结合,建立了一种HPTLC-DPPH联用测定保健品中芦丁的方法,并考察了DPPH显色反应时间、流动相配比、光密度扫描参数对色谱分离、扫描效果的影响,同时测试了该方法的回收率和精密度。结果显示,该方法的标准添加回收率为82%~97%,精密度RSD<10%,且可消除保健品中复杂基质的干扰,适用于保健品中芦丁的快速测定。 展开更多
关键词 芦丁 保健品 HPTLC DPPH
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Antidepressant activity and HPTLC fingerprinting of stearic acid in different days of wheat seedlings 预览
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作者 Palanisamy Ravikumar Muthusamy Jeyam 《粮油科技:英文版》 2019年第1期6-10,共5页
Depression is a chronic,recurring and potentially life threatening illness and affects up to 20%of the world population and in the year 2020,depression will become the second most common disease in the world.To find t... Depression is a chronic,recurring and potentially life threatening illness and affects up to 20%of the world population and in the year 2020,depression will become the second most common disease in the world.To find the remedy from nutraceuticals,the present study was designed to evaluate the antidepressant activity of stearic acid(SA)and to quantify its maximum content in different dayold wheat seedlings and wheat grains.Forced swimming test(FST)and tail suspension test(TST)were done to evaluate the antidepressant activity of SA.HPTLC fingerprinting of SA was done in different days(3,5 and 7 d)of wheat seedlings and wheat grains to quantify its maximum content.In the antidepressant study,when compared with the control(326.67±3.02 s),SA showed potential antidepressant activity in TST(131.67±2.60 s)and SA also showed very good antidepressant activity in FST(124.83±5.37 s)when compared with the control(215.83±6.64 s).In HPTLC fingerprinting,the maximum content of SA was identified in 3-d-old wheat seedlings(89.43μg)when compared to wheat grains(84.69μg),5-d-old(86.43μg)and 7-d-old(85.32μg)wheat seedlings.Hence,the present study concludes that SA has a potential antidepressant activity and 3-d-old wheat seedlings are the essential sources of SA among the different dayold wheat seedlings. 展开更多
关键词 Depression Forced SWIMMING TEST TAIL suspension TEST Stearic acid HPTLC fingerprinting 3-d-old wheat SEEDLINGS
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Antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus
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作者 Shohini Chakraborty Nashra Afaq +1 位作者 Neelam Singh Sukanta Majumdar 《结合医学学报：英文版》 CAS CSCD 2018年第5期350-357,共8页
关键词 葡萄球菌 抗菌剂 大麻 印度 MRSA HPTLC 生物化学 青霉素
草豆蔻及其近缘种药用植物的高效薄层色谱指纹图谱研究 被引量：2
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作者 屈敏红 李倩 +1 位作者 伍彩红 丁平 《中国药学杂志》 CSCD 北大核心 2018年第4期258-262,共5页
目的建立不同商品来源的草豆蔻挥发油和黄酮类成分高效薄层色谱指纹图谱，为其质量控制提供依据，并以草豆蔻为对照，对其近缘种常用药用植物进行考察。方法应用硅胶GF254高效预制薄层板，挥发油成分以甲苯-乙酸乙酯（9：1）为展开剂，... 目的建立不同商品来源的草豆蔻挥发油和黄酮类成分高效薄层色谱指纹图谱，为其质量控制提供依据，并以草豆蔻为对照，对其近缘种常用药用植物进行考察。方法应用硅胶GF254高效预制薄层板，挥发油成分以甲苯-乙酸乙酯（9：1）为展开剂，喷以5％香草醛浓硫酸溶液，105℃加热至斑点清晰，日光下观察；黄酮类成分以甲苯．乙酸乙酯．乙酸（8：1．5：0．5）为展开剂，喷以5％三氯化铝乙醇液，105℃加热数分钟后，置于365nm紫外光下检视。将显色后的薄层色谱图导入CHROMAP1．5色谱指纹图谱系统解决方案软件生成共有模式，进行相似度分析。结果草豆蔻挥发油成分和黄酮类成分薄层色谱指纹图谱分别由10个和7个色谱峰组成，并分别指认了主要成分桉油精和山姜素。结论通过HPTLC指纹图谱考察，不同商品来源的草豆蔻药材斑点质量差别较小；草豆蔻及其近缘种常用药用植物的挥发油成分和黄酮类成分均差别显著。该方法可以快速、有效地对草豆蔻进行鉴别和质量评价。 展开更多
关键词 草豆蔻 近缘种药用植物 挥发油 黄酮 高效薄层色谱指纹图谱
3种新烟碱类杀虫剂在蜂蜜中HPTLC残留分析方法的研究
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作者 梁睿 岳永德 汤锋 《安徽农业大学学报》 CSCD 2018年第4期735-739,共5页
建立了吡虫啉、噻虫胺和噻虫嗪在蜂蜜中残留的高效薄层色谱（HPTLC）检测方法，用乙腈机械震荡提取后涡旋并离心样品，于C-18固相萃取柱净化，CAMAG Linomat Ⅳ点样仪点样，二氯甲烷∶乙酸乙酯= 3∶1 （V∶V）混合溶液单向上行展开，于2... 建立了吡虫啉、噻虫胺和噻虫嗪在蜂蜜中残留的高效薄层色谱（HPTLC）检测方法，用乙腈机械震荡提取后涡旋并离心样品，于C-18固相萃取柱净化，CAMAG Linomat Ⅳ点样仪点样，二氯甲烷∶乙酸乙酯= 3∶1 （V∶V）混合溶液单向上行展开，于254 nm波长进行HPTLC检测。结果表明，当3种农药在蜂蜜中的添加浓度分别0.05、0.5和1.0 mg·kg-1时，添加回收率分别为71.58%～101.25%、72.29%～109.47%和79.88%～108.92%，相对标准偏差分别为9.31%～14.47%、7.79%～12.86%和9.24%～14.23%，均满足农残分析的基本要求。 展开更多
关键词 新烟碱类杀虫剂 蜂蜜 高效薄层色谱 农残分析
何首乌与制何首乌的高效薄层色谱指纹图谱研究 被引量：2
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作者 刘美廷 李倩 +2 位作者 屈敏红 伍彩红 丁平 《华西药学杂志》 CAS CSCD 2018年第2期193-196,共4页
目的 建立何首乌与制何首乌的高效薄层色谱（HPTLC）指纹图谱,并对不同干燥方式、生长年限、产地来源的药材进行比较分析。方法 采用甲醇超声提取,浓缩滤液的方式提取样品。应用硅胶GF254高效预制薄层板,以三氯甲烷-甲醇-水（7∶3∶0.5... 目的 建立何首乌与制何首乌的高效薄层色谱（HPTLC）指纹图谱,并对不同干燥方式、生长年限、产地来源的药材进行比较分析。方法 采用甲醇超声提取,浓缩滤液的方式提取样品。应用硅胶GF254高效预制薄层板,以三氯甲烷-甲醇-水（7∶3∶0.5）和三氯甲烷-丙酮-甲醇-水（6.5∶2.5∶1.5∶0.5）为展开剂,二次展开,置365 nm下检视。将所得薄层色谱图导入Chromap1.5色谱指纹图谱系统解决方案软件中生成共有模式,进行相似度分析。结果 何首乌与制何首乌的薄层色谱指纹图谱分别由7个与6个（不同产地制何首乌为5个）共有色谱峰组成。通过远红外干燥后的何首乌斑点信息丰富,整体较清晰;不同年限、产地的药材在成分上存在差异。结论 高效薄层色谱法可快速、简便、有效地鉴别何首乌与制何首乌,为评价药材内在质量的方法提供了依据。 展开更多
关键词 何首乌 制何首乌 高效薄层色谱 相似度分析 共有模式 鉴别
4种不同基原穿山甲炮制品的鉴定 被引量：1
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作者 刘逊 张华峰 +2 位作者 刘雪梅 朱缨 崔小兵 《中药材》 北大核心 2017年第3期585-588,共4页
目的：建立穿山甲饮片鉴定方法。方法：运用砂烫法对穿山甲进行炮制,首先从形状、大小、色泽、质地特征等方面对不同基原炮山甲饮片性状进行观察与描述;其次,采用二次展开法对高效薄层板进行扫描,利用Camag TLC Scanner4 System软件进... 目的：建立穿山甲饮片鉴定方法。方法：运用砂烫法对穿山甲进行炮制,首先从形状、大小、色泽、质地特征等方面对不同基原炮山甲饮片性状进行观察与描述;其次,采用二次展开法对高效薄层板进行扫描,利用Camag TLC Scanner4 System软件进行分析,同时采用TOF MS-IDA-MS/MS对炮山甲进行成分分析,使用Markerview等分析软件,通过PCA分析,寻找各来源穿山甲炮制品之间的差异。结果：炮制品中,穿山甲、马来穿山甲的性状及化学成分较为类似,可归为一类;树穿山甲、印度穿山甲炮制品的性状及化学成分差异较大,各自归为一类,同时各来源穿山甲经炮制后均产生丝-酪环二肽,可以作为鉴定穿山甲炮制品的共性指标成分。结论：性状、HPTLC结合TOF MS-IDA-MS/MS,可有效鉴别不同基原的穿山甲炮制品,为穿山甲的鉴定提供科学依据。 展开更多
关键词 穿山甲 性状 HPTLC TOF MS-IDA-MS/MS 鉴定
人参对照提取物的应用研究——人参皂苷成分的质量分析 被引量：1
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作者 郭隆钢 巫少娟 +3 位作者 孙帅 许舜军 许铮弟 谢培山 《中药新药与临床药理》 CSCD 北大核心 2017年第6期771-776,共6页
目的使用人参对照提取物（Ginseng Extract Reference Substance,GS ERS）对人参样品进行定性鉴别及定量分析。方法使用薄层色谱法、硅胶高效薄层板,以氯仿-乙酸乙酯-甲醇-水（15∶40∶22∶10）10℃以下分层的下层溶剂为展开剂,对人参... 目的使用人参对照提取物（Ginseng Extract Reference Substance,GS ERS）对人参样品进行定性鉴别及定量分析。方法使用薄层色谱法、硅胶高效薄层板,以氯仿-乙酸乙酯-甲醇-水（15∶40∶22∶10）10℃以下分层的下层溶剂为展开剂,对人参样品进行定性鉴别。采用Kromasil 100-5 C_（18）色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-水,梯度洗脱;流速1.0 m L·min-1,柱温25℃,检测波长203 nm,进样量10μL。用已知含量的GS ERS外标法测定人参样品中人参皂苷Rg_1、Re、Rf、Rb_1、Rc、Rb_2、Rd 7个成分的含量。结果高效薄层色谱显示GS ERS与人参药材图谱具有一致性,且与西洋参有明显区别;使用GS ERS外标法测定人参及西洋参样品中的人参皂苷成分,结果与使用化学对照品外标法测定结果一致,两种方法相对平均偏差在0.02%~2.90%之间。结论 GS ERS用于人参样品的定性鉴别以及人参药材中人参皂苷Rg1,Re,Rf,Rb1,Rc,Rb2,Rd 7个成分的含量测定,方便有效,经济实惠。对照提取物符合中药整体性和复杂性的特点,更适合中药整体质量控制。 展开更多
关键词 人参对照提取物 人参皂苷 含量测定 高效薄层色谱 高效液相色谱
砂仁及其近缘药用植物的高效薄层色谱指纹图谱研究 被引量：2
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作者 邵艳华 刘美廷 +2 位作者 杨一帆 于现阔 丁平 《中国药学杂志》 CAS CSCD 北大核心 2017年第3期188-192,共5页
目的 对砂仁及其近缘药用植物的挥发油成分进行高效薄层色谱指纹图谱研究,以期解决砂仁的专属性鉴别,并为其质量控制提供依据。方法 采用高效薄层色谱法,控制相对湿度为67%,环己烷-三氯甲烷-乙酸乙酯（13∶2∶2）为展开剂,5%香草醛硫酸... 目的 对砂仁及其近缘药用植物的挥发油成分进行高效薄层色谱指纹图谱研究,以期解决砂仁的专属性鉴别,并为其质量控制提供依据。方法 采用高效薄层色谱法,控制相对湿度为67%,环己烷-三氯甲烷-乙酸乙酯（13∶2∶2）为展开剂,5%香草醛硫酸溶液显色,于可见光下检视,薄层色谱图用Chromap 1.5色谱软件进行分析。结果 建立了由9个特征斑点构成的砂仁挥发油类成分的高效薄层色谱指纹图谱,并指认出主要成分乙酸龙脑酯。通过HPTLC指纹图谱考察,不同存放时间及不同产地的砂仁药材质量参差不齐;常见砂仁混伪品间质量差别显著;砂仁及姜科其他近缘种常用药用植物挥发油类成分的化学组成亦差异明显。结论 该方法可以有效地鉴别砂仁,并评价其质量。 展开更多
关键词 砂仁 混伪品 近缘药用植物 挥发油 高效薄层色谱法
Quantitative determination of vitexin in Passiflora foetida Linn. leaves using HPTLC
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作者 Aussavashai Shuayprom Donruedee Sanguansermsri +2 位作者 Phanchana Sanguansermsri Ian Hamilton Fraser Nalin Wongkattiya 《亚太热带生物医学杂志：英文版》 CAS 2016年第3期216-220,共5页
Objective: To establish a simple, rapid, precise and accurate high performance thin layer chromatography(HPTLC) method with densitometric detection for the determination of vitexin in Passiflora foetida Linn.(P. foeti... Objective: To establish a simple, rapid, precise and accurate high performance thin layer chromatography(HPTLC) method with densitometric detection for the determination of vitexin in Passiflora foetida Linn.(P. foetida).Methods: Ethanolic extract of the plant leaf powder was used for the experimental work.Separation was performed on silica gel 60 F254 HPTLC plates with ethyl acetate:methanol: distilled water: formic acid in the proportion of 50:2:3:6(v/v), as the mobile phase. The determination was carried out using the densitometric absorbance mode at340 nm.Results: Vitexin response was linear over the range of 2.5–17.5 mg/m L with a correlation coefficient of 0.996. Intraday and interday precision studies showed the relative SD was< 3%. Accuracy of the method was determined and the average recovery was 100.3%.The limit of quantitation and limit of detection were 0.879 and 0.290 mg/m L, respectively.The contents of vitexin in P. foetida leaf extracts were within the range of 0.030%–0.310%.Conclusions: The method was evaluated for sensitivity, accuracy, precision and reproducibility. Each analysis by HPTLC is less expensive than current methods. This method is suitable for routine quality control of raw material of the leaves of P. foetida extract and its products. 展开更多
关键词 HPTLC Thin layer CHROMATOGRAPHY VITEXIN PASSIFLORA foetida LINN
Free radical scavenging potential and HPTLC analysis of Indigofera tinctoria linn （Fabaceae） 预览 被引量：1
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作者 Sakthivel Srinivasan Wankupar Wankhar +1 位作者 Sheeladevi Rathinasamy Ravindran Rajan 《药物分析学报：英文版》 CAS 2016年第2期125-131,共7页
The objective of this study was to evaluate the free radical scavenging potential and high performance thin layer chromatography（HPTLC） fingerprinting of Indigofera tinctoria（I. tinctoria）. Phytochemical analysis ... The objective of this study was to evaluate the free radical scavenging potential and high performance thin layer chromatography（HPTLC） fingerprinting of Indigofera tinctoria（I. tinctoria）. Phytochemical analysis was carried out using standard methods, and free radical scavenging activity of the plant was determined using 2,2-diphenyl-1-picrylhydrazy（DPPH）, nitric oxide（NO） and superoxide anion（O2-）radical scavenging capacities. HPTLC plate was kept in CAMAG TLC Scanner 3 and the Rf values at fingerprint data were recorded by WINCATS software. Aqueous extract of I. tinctoria reliably showed the total phenolics（267.2±2.42 mg/g）, flavonoids（75.43±3.36 mg/g） and antioxidants（349.11±8.04 mg/g）. The extract was found to have DPPH（52.08%）, NO（23.12%） and O2-（26.79%） scavenging activities at the concentration of 250 μg/m L and the results were statistically signi ficant compared with ascorbic acid standard（p<0.05）. HPTLC results con firmed that the extract contained several potential active components such as phenols, flavonoids, saponins and terpenoids as the slides revealed multi-colored bands of varying intensities. This study confirmed that the plant had multipotential antioxidant and free radicals scavenging activities. 展开更多
关键词 清除自由基 薄层色谱分析 DPPH自由基 高效薄层色谱 豆科 木蓝 化学成分分析 水提取物
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无机离子对嗜盐放线菌株YIM90615代谢产物多样性的影响 预览 被引量：1
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作者 张正芸 陆晓菊 +2 位作者 官会林 唐蜀昆 丁章贵 《云南大学学报：自然科学版》 CAS CSCD 北大核心 2015年第1期147-154,共8页
为了研究Na Cl浓度、金属离子（Cr^7＋、Mg^2＋及K^＋）、氯化钪及卤素（I^-、Br^-及F^-）对嗜盐放线菌株YIM90615液体发酵过程中次级代谢产物多样性的影响,作者采用HPTLC（high performance thin layer chromatography）技术,结合紫外... 为了研究Na Cl浓度、金属离子（Cr^7＋、Mg^2＋及K^＋）、氯化钪及卤素（I^-、Br^-及F^-）对嗜盐放线菌株YIM90615液体发酵过程中次级代谢产物多样性的影响,作者采用HPTLC（high performance thin layer chromatography）技术,结合紫外（254 nm）检测、碘及硫酸-茴香醛显色方法,对该菌代谢产物的HPTLC图谱进行了定性分析.结果显示,Na Cl添加质量浓度为100 g/L时该菌株发酵效果最佳,有利于其代谢产物的多样性及量的积累;Cr^7＋和K^＋有利于其次生代谢产物的产生;1 g/L的氯化钪能增加其次生代谢产物的丰富性;F-对其次生代谢产物多样性有较好的促进作用.试验结果表明外源添加一定浓度和种类的盐离子有助于提升YIM 90615液体发酵过程中次生代谢产物多样性,为新型抗生素及其它有用化合物的产生创造合适条件,同时有助于嗜盐放线菌发酵培养基的筛选工作. 展开更多
关键词 嗜盐放线菌 液体发酵 次级代谢产物 高效薄层色谱(HPTLC)
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Analysis of Multi-ingredient Food Supplements by Fingerprint HPTLC Approach 预览
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作者 Marcello Nicoletti Chiara Toniolo 《化学与化工：英文版》 2015年第4期239-244,共6页
关键词 色谱指纹图谱 多成分 HPTLC 食品补充剂 色谱分析方法 植物性 食品分析 成分测定
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HPTLC Analysis of multi-ingredient traditional Chinese medicine supplement 预览
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作者 MartinCAI SheenaGOH +1 位作者 LengNEO FengTIAN 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2015年第S1期93-94,共2页
OBJECTIVE Analysis of traditional Chinese medicinal(TCM)supplements has always been a laborious task,particularly in the case of multi-ingredient formulations.Traditionally,herbal extracts are analyzed using one or fe... OBJECTIVE Analysis of traditional Chinese medicinal(TCM)supplements has always been a laborious task,particularly in the case of multi-ingredient formulations.Traditionally,herbal extracts are analyzed using one or few markers compounds.In the recent years,however,pharmaceutical companies are introducing health supplements of TCM ingredients to cater to the needs of consumers in the fast-paced society in this age.As such,new problems arise in the aspects of composition identification as well as quality analysis.In most cases of products or supplements formulated with multiple TCM herbs,the chemical composition and nature of each raw material differs greatly from the others in the formulation.This results in a requirement for individual analytical processes in order to identify the marker compounds in the various botanicals.The aim of this study is to establish a rapid and low cost analytical approach using high performance thin layer chromatography(HPTLC)for the analysis of 3 key components of the LAC Liver Protector,a supplement with TCM formulation aimed at improving liver health.METHODS The TCM herbs were extracted with methods derived from Chinese Pharmacopeia standards.The herbal extracts were then applied to HPTLC plates using CAMAG Linomat 5 and developed using fully saturated twin-trough chromatographic chambers.The developed plates were derivatized and documented with CAMAG TLC Visualizer.The images were processed with CAMAG winCATS software to calculate the Rf values and confirm the presence of key constituents of the herbs.RESULTS The 3 key components of LAC Liver Protector product,namely Radix notoginseng,Rhizoma alismatis and Radix paeoniae alba/rubra were identified in all 6 batches of products tested.CONCLUSION With the increasing trend of small and medium-sized enterprises(SMEs)bringing natural products and health supplements into the market,it is crucial that the qualities of both raw materials and end products be well-assured for the protection of consumers.With the technology of HPTLC,science c 展开更多
关键词 TLC HPTLC RADIX notoginseng Rhizoma alismatis Radi
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Preparation of κ-carra-Oligosaccharides with Microwave Assisted Acid Hydrolysis Method 预览
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作者 LIGuangsheng 《中国海洋大学学报：英文版》 SCIE CAS 2015年第2期345-349,共5页
帮助的酸水解作用被建立准备 -carra-oligosaccharides 的微波的一个快速的方法。最佳的水解作用条件被直角的测试决定。oligosaccharides 的聚合(DP ) 的度被高效检测薄层层析(HPTLC ) 和 polyacrylamide 胶化电气泳动(页) 。就 HPTLC... 帮助的酸水解作用被建立准备 -carra-oligosaccharides 的微波的一个快速的方法。最佳的水解作用条件被直角的测试决定。oligosaccharides 的聚合(DP ) 的度被高效检测薄层层析(HPTLC ) 和 polyacrylamide 胶化电气泳动(页) 。就 HPTLC 和页的结果而言，微波的最佳条件帮助了酸水解作用被决定。-carrageenan 的集中是 5 mg mL < 啜 class= “ a-plus-plus ” > 1 </sup> ；反应答案被适应 pH 3 与冲淡盐酸酸；答案为在这些 indic 燬?燬 的 15 个变化是在在 100 点的微波照耀下面的 hydrolyzed 吗？？ 展开更多
关键词 酸水解法 微波辅助 寡糖 制备 聚丙烯酰胺凝胶电泳 HPTLC ESI-MS PAGE
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Fingerprinting of the Acacia nilotica （L.） Bark Extract Having Antibacterial Property 预览
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作者 Jayshree D. Patel Nirmal S. Sahay Vipin Kumar 《健康科学：英文版》 2015年第3期123-127,共5页
关键词 保健法 医学心理学 心理卫生 心理健康
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复方补乌糖浆的HPTLC图谱研究 预览
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作者 陈莉 王盛 +2 位作者 夏伦祝 孟楣 郭艳丽 《中成药》 CAS CSCD 北大核心 2014年第9期1886-1890,共5页
目的研究复方补乌糖浆（补骨脂、川芎、当归、生首乌等）高效薄层色谱,用于质量控制。方法复方补乌糖浆用碳酸钠溶液溶解,乙酸乙酯和正丁醇萃取,不同极性部位进行特征图谱研究,用Chromafinger软件进行相似度分析。结果对10批复方补乌糖... 目的研究复方补乌糖浆（补骨脂、川芎、当归、生首乌等）高效薄层色谱,用于质量控制。方法复方补乌糖浆用碳酸钠溶液溶解,乙酸乙酯和正丁醇萃取,不同极性部位进行特征图谱研究,用Chromafinger软件进行相似度分析。结果对10批复方补乌糖浆进行了考察,乙酸乙酯部位确定了12个共有峰,各批样品特征图谱相似度均大于0.99;正丁醇部位确定了10个共有峰,各批样品特征图谱相似度均大于0.97,共有峰中包括补骨脂素、异补骨脂素、大黄素、丹参酮ⅡA、丹酚酸B和阿魏酸等6个色谱峰。结论此方法未发现复方补乌糖浆中左旋紫草素、齐墩果酸和藁本内酯。 展开更多
关键词 复方补乌糖浆 HPTLC图谱 质量控制
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Gastroprotective effect of the iridoid fraction from Barleria prionitis leaves on experimentally-induced gastric ulceration
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作者 Sunil K.Jaiswal Mukesh K.Dubey +1 位作者 Sanjib Das Ch.V.Rao 《中国天然药物》 SCIE CAS CSCD 2014年第10期738-744,共7页
AIM: To study the gastroprotective effect and in vivo antioxidant potential of a standardized iridoid fraction from B. prionitis leaves(BPE) against different gastric ulcer models in rats. METHOD: The standardized iri... AIM: To study the gastroprotective effect and in vivo antioxidant potential of a standardized iridoid fraction from B. prionitis leaves(BPE) against different gastric ulcer models in rats. METHOD: The standardized iridoid fraction from BPE at 50, 100, and 200 mg/kg body weight was administered orally, twice daily for 5 days for prevention from aspirin, ethanol, cold-restraint stress(CRS), and pylorus ligation(PL)-induced ulcers. Estimation of the antioxidant enzyme activity was carried out in a CRS-induced ulcer model, and various gastric secretion parameters including volume of gastric juice, acid output, and pH value were estimated in the PL-induced ulcer model. RESULTS: BPE showed a dose-dependent ulcer protective effect in PL(18.67%–66.26% protection), aspirin(24.65%–63.25% protection), CRS(20.77%–59.42% protection), and EtOH(16.93%–77.04% protection)-induced ulcers. BPE treatment in PL-rats showed a decrease in acid-pepsin secretion, and enhanced mucin and mucosal glycoproteins. However, BPE reduced the ulcer index with significant decrease in LPO(P < 0.01–0.001), SOD(P < 0.01–0.001), and an increase in CAT(P < 0.01–0.001), activity in the CRS-induced model. CONCLUSION: The data shows that the iridoid fraction from BPE possesses anti-ulcerogenic and antioxidant potential. 展开更多
关键词 环烯醚萜 HPTLC 抗溃疡活性 实现
Chromatographic finger print analysis of anti-inflammatory active extract fractions of aerial parts of Tribulus terrestris by HPTLC technique
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作者 Mona Salih Mohammed Mohamed Fahad Alajmi +3 位作者 Perwez Alam Hassan Subki Khalid Abelkhalig Muddathir Mahmoud Wadah Jamal Ahmed 《亚太热带生物医学杂志：英文版》 2014年第3期203-208,共6页
Objective:To develop HPTLC fingerprint profile of anti-inflammatory active extract fractions of Tribulus terrestris(family Zygophyllaceae).Methods:The anti-inflammatory activity was tested for the methanol and its fr... Objective:To develop HPTLC fingerprint profile of anti-inflammatory active extract fractions of Tribulus terrestris(family Zygophyllaceae).Methods:The anti-inflammatory activity was tested for the methanol and its fractions(chloroform,ethyl acetate,n-butanol and aqueous)and chloroform extract of Tribulus terrestris(aerial parts)by injecting different groups of rats(6 each)with carrageenan in hind paw and measuring the edema volume before and 1,2 and 3 h after carrageenan injection.Control group received saline i.p.The extracts treatment was injected i.p.in doses of 200 mg/kg 1 h before carrageenan administration.Indomethacin(30 mg/kg)was used as standard.HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator.TLC scanner 3.Reprostar 3,CAMAG ADC 2 and WIN CATS-4 software for the active fractions of chloroform fraction of methanol extract.Results:The methanol extract showed good antiedematous effect with percentage of inhibition more than 72%,indicating its ability to inhibit the inflammatory mediators.The methanol extract was re-dissolved in 100 mL of distilled water and fractionated with chloroform,ethyl acetate and n-butanol.The four fractions(chloroform,ethyl acetate,n-butanol and aqueous)were subjected to anti-inflammatory activity.Chloroform fraction showed good anti-inflammatory activity at dose of 200 mg/kg.Chloroform fraction was then subjected to normal phase silica gel column chromatography and eluted with petroleum ether-chloroform,chloroform-ethyl acetate mixtures of increasing polarity which produced 15 fractions(F1-F15).Only fractions F1,F2,F4,F5,F7,F9,F11 and F14 were found to be active,hence these were analyzed with HPTLC to develop their finger print profile.These fractions showed different spots with different R_f values.Conclusions:The different chloroform fractions F1,F2,F4,F5,F7,F9,F11 and F14 revealed 4,7,7,8,9,7,7 and 6 major spots,respectively.The results obtained in this experiment strongly support and validate the traditional uses of this Sudanese medi 展开更多
关键词 Tribulus terrestris FINGER PRINT ANTI-INFLAMMATORY Standardization HPTLC
Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels
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作者 Prawez Alam Aftab Alam +1 位作者 Khalid Anwer Saleh I Alqasoumi 《亚太热带生物医学杂志：英文版》 2014年第4期262-266,共5页
Objective:To develop a simple,selective,sensitive and accurate high-performance thin layer chromatography（HPTLC）method to determine the quantity of hesperidin in different varieties of citrus fruits.Methods:The met... Objective:To develop a simple,selective,sensitive and accurate high-performance thin layer chromatography（HPTLC）method to determine the quantity of hesperidin in different varieties of citrus fruits.Methods:The method was carried out in aluminum-backed silica gel 60 F<sub>254</sub>plates with ethyl acetate-methanol-water 15:3:2（%,v/v）as mobile phase.Results:A compact band was obtained for hesperidin at R<sub>f</sub> value of（0.40±0.04）.The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.9986was indicative of good linear dependence of peak area on concentration.Limit of detection（8.87ng/spot）,limit of quantification（23.21 ng/spot）,accuracy（less than 2%）and recovery（ranging from98.55-99.38）were found satisfactory.Conclusions:The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient. 展开更多
关键词 HPTLC CITRUS HESPERIDIN
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