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MicroRNA expression in the hippocampal CA1 region under deep hypothermic circulatory arrest 预览
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作者 Xiao-Hua Wang Dong-Xu Yao +7 位作者 Xiu-Shu Luan Yu Wang Hai-Xia Liu Bei Liu Yang Liu Lei Zhao Xun-Ming Ji Tian-Long Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期2003-2010,共8页
Using deep hypothermic circulatory arrest, thoracic aorta diseases and complex heart diseases can be subjected to corrective procedures. However, mechanisms underlying brain protection during deep hypothermic circulat... Using deep hypothermic circulatory arrest, thoracic aorta diseases and complex heart diseases can be subjected to corrective procedures. However, mechanisms underlying brain protection during deep hypothermic circulatory arrest are unclear. After piglet models underwent 60 minutes of deep hypothermic circulatory arrest at 14°C, expression of microRNAs(miRNAs) was analyzed in the hippocampus by microarray. Subsequently, TargetScan 6.2, RNA22 v2.0, miRWalk 2.0, and miRanda were used to predict potential targets, and gene ontology enrichment analysis was carried out to identify functional pathways involved. Quantitative reverse transcription-polymerase chain reaction was conducted to verify miRNA changes. Deep hypothermic circulatory arrest altered the expression of 35 miRNAs. Twenty-two miRNAs were significantly downregulated and thirteen miRNAs were significantly upregulated in the hippocampus after deep hypothermic circulatory arrest. Six out of eight targets among the differentially expressed miRNAs were enriched for neuronal projection(cyclin dependent kinase, CDK16 and SLC1 A2), central nervous system development(FOXO3, TYRO3, and SLC1 A2), ion transmembrane transporter activity(ATP2 B2 and SLC1 A2), and interleukin-6 receptor binding(IL6 R)– these are the key functional pathways involved in cerebral protection during deep hypothermic circulatory arrest. Quantitative reverse transcription-polymerase chain reaction confirmed the results of microarray analysis. Our experimental results illustrate a new role for transcriptional regulation in deep hypothermic circulatory arrest, and provide significant insight for the development of miRNAs to treat brain injuries. All procedures were approved by the Animal Care Committee of Xuanwu Hospital, Capital Medical University, China on March 1, 2017(approval No. XW-INI-AD2017-0112). 展开更多
关键词 nerve REGENERATION cerebral protection deep hypothermic circulatory ARREST gene ontology enrichment analysis microRNA hippocampus POST-TRANSCRIPTIONAL expression MICROARRAY BIOINFORMATICS neural REGENERATION
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Liver graft preservation methods during cold ischemia phase and normothermic machine perfusion 预览
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作者 Konstantin Y Tchilikidi 《世界胃肠外科杂志:英文版(电子版)》 2019年第3期126-142,共17页
The growing demand for donor organs requires measures to expand donor pool. Those include extended criteria donors, such as elderly people, steatotic livers, donation after cardiac death, etc. Static cold storage to r... The growing demand for donor organs requires measures to expand donor pool. Those include extended criteria donors, such as elderly people, steatotic livers, donation after cardiac death, etc. Static cold storage to reduce metabolic requirements developed by Collins in late 1960s is the mainstay and the golden standard for donated organ protection. Hypothermic machine perfusion provides dynamic organ preservation at 4°C with protracted infusion of metabolic substrates to the graft during the ex vivo period. It has been used instead of static cold storage or after it as short perfusion in transplant center. Normothermic machine perfusion (NMP) delivers oxygen, and nutrition at physiological temperature mimicking regular environment in order to support cellular function. This would minimize effects of ischemia/reperfusion injury. Potentially, NMP may help to estimate graft functionality before implantation into a recipient. Clinical studies demonstrated at least its non-inferiority or better outcomes vs static cold storage. Regular grafts donated after brain death could be safely preserved with convenient static cold storage. Except for prolonged ischemia time where hypothermic machine perfusion started in transplant center could be estimated to provide possible positive reconditioning effect. Use of hypothermic machine perfusion in regular donation instead of static cold storage or in extended criteria donors requires further investigation. Multicenter randomized clinical trial supposed to be completed in December 2021. Extended criteria donors need additional measures for graft storage and assessment until its implantation. NMP is actively evaluating promising method for this purpose. Future studies are necessary for precise estimation and confirmation to issue clinical practice recommendations. 展开更多
关键词 Liver GRAFT PRESERVATION GRAFT PRESERVATION solutions Static cold storage Hypothermic MACHINE PERFUSION Normothermic MACHINE PERFUSION Ischemia-reperfusion injury Marginal GRAFTS DONATION after cardiac death Extended criteria donors Transpla complications
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Predictive Score Model for Delayed Graft Function Based on Hypothermic Machine Perfusion Variables in Kidney Transplantation
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作者 Chen-Guang Ding Yang Li +6 位作者 Xiao-Hui Tian Xiao-Jun Hu Pu-Xun Tian Xiao-Ming Ding He-Li Xiang Jin Zheng Wu-Jun Xue 《中华医学杂志:英文版》 SCIE CAS CSCD 2018年第22期2651-2657,共7页
Background:Hypothermic machine perfusion (HMP)is being used more often in cardiac death kidney transplantation;however,the significance of assessing organ quality and predicting delayed graft function (DGF)by HMP para... Background:Hypothermic machine perfusion (HMP)is being used more often in cardiac death kidney transplantation;however,the significance of assessing organ quality and predicting delayed graft function (DGF)by HMP parameters is still controversial.Therefore, we used a readily available HMP variable to design a scoring model that can identify the highest risk of DGF and provide the guidance and advice for organ allocation and DCD kidney assessment. Methods:From September 1,2012 to August 31,2016,366 qualified kidneys were randomly assigned to the development and validation cohorts in a 2:1 distribution.The HMP variables of the development cohort served as candidate univariate predictors for DGF.The independent predictors of DGF were identified by multivariate logistic regression analysis with a P <0.05.According to the odds ratios (ORs)value,each HMP variable was assigned a weighted integer,and the sum of the integers indicated the total risk score for each kidney.The validation cohort was used to verify the accuracy and reliability of the scoring model. Results:HMP duration (OR =1.165,95% confidence interval [CI]:1.008-1.360,P =0.043),resistance (OR =2.190,95% CI:1.032-10.20,P <0.001),and flow rate (OR =0.931,95%CI:0.894-0.967,P =0.011)were the independent predictors of identified DGF.The HMP predictive score ranged from 0 to 14,and there was a clear increase in the incidence of DGF,from the low predictive score group to the very high predictive score group.We formed four increasingly serious risk categories (scores 0-3,4-7,8-11,and 12-14) according to the frequency associated with the different risk scores of DGF.The HMP predictive score indicates good discriminative power with a c-statistic of 0.706 in the validation cohort,and it had significantly better prediction value for DGF compared to both terminal flow (P =0.012)and resistance (P =0.006). Conclusion:The HMP predictive score is a good noninvasive tool for assessing the quality of DCD kidneys,and it is potentially useful for physicians in making optimal de 展开更多
关键词 DELAYED GRAFT Function DONATION after CARDIAC Death Hypothermic Machine PERFUSION Kidney Transplantation
Beneficial Effect of Moderately Increasing Hypothermic Machine Perfusion Pressure on Donor after Cardiac Death Renal Transplantation
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作者 Chen-Guang Ding Pu-Xun Tian +8 位作者 Xiao-Ming Ding He-Li Xiang Yang Li Xiao-Hui Tian Feng Han Qian-Hui Tai Qian-Long Liu Jin Zheng Wu-Jun Xue 《中华医学杂志:英文版》 SCIE CAS CSCD 2018年第22期2676-2682,共7页
Background:Vascular resistance and flow rate during hypotherrnic machine perfusion (HMP)of kidneys is correlated with graft function. We aimed to determine the effects of increasing HMP pressure versus maintaining the... Background:Vascular resistance and flow rate during hypotherrnic machine perfusion (HMP)of kidneys is correlated with graft function. We aimed to determine the effects of increasing HMP pressure versus maintaining the initial pressure on kidney transplantation outcomes. Methods:We retrospectively reviewed the data of 76 primary transplantation patients who received HMP-preserved kidneys from 48 donors after cardiac death between September 1,2013,and August 31,2015.HMP pressure was increased from 30 to 40mmHg (1mmHg =0.133kPa)in kidneys with poor flow and/or vascular resistance (increased pressure [IP]group;36 patients);otherwise,the initial pressure was maintained (constant pressure group;40 patients).Finally,the clinical characteristics and transplantation outcomes in both groups were assessed. Results:Delayed graft function (DGF)incidence,1-year allograft,patient survival,kidney function recovery time,and serum creatinine level on day 30 were similar in both groups,with improved flow and resistance in the IP group.Among patients with DGF,kidney function recovery time and DGF duration were ameliorated in the IP group.Multivariate logistic regression analysis revealed that donor hypertension (odds ratio [OR]:1.43,95%confidence interval [CI]:1.02-2.06,P =0.035),donor terminal serum creatinine (OR:1.27,95%C7:1.06-1.62,P =0.023),warm ischemic time (OR:3.45,95%CI:1.97-6.37,P =0.002),and terminal resistance (OR:3.12,95%CI:1.76-6.09,P =0.012)were independent predictors of DGF.Cox proportional hazards analysis showed that terminal resistance (hazard ratio:2.06,95%C1:1.32-5.16,P =0.032)significantly affected graft survival. Conclusion:Increased HMP pressure improves graft perfusion but does not affect DGF incidence or 1-year graft survival. 展开更多
关键词 DELAYED GRAFT Function DONOR AFTER CARDIAC Death Hypothermic Machine PERFUSION Kidney Transplantation
低温环境下容量控制性失血性休克猪的丙泊酚药代动力学研究 预览
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作者 夏艳萍 陈克研 +1 位作者 张铁铮 刁玉刚 《实用药物与临床》 CAS 2016年第4期393-397,共5页
目的探讨低温环境下容量控制性失血性休克猪体内丙泊酚药代动力学的特点。方法24头健康巴马小型猪,应用随机数字表法将其随机分为4组,每组6头,分别为常温不休克组(C组)、常温休克组(CS组)、低温不休克组(D组)、低温休克组(DS组... 目的探讨低温环境下容量控制性失血性休克猪体内丙泊酚药代动力学的特点。方法24头健康巴马小型猪,应用随机数字表法将其随机分为4组,每组6头,分别为常温不休克组(C组)、常温休克组(CS组)、低温不休克组(D组)、低温休克组(DS组)。建立低温失血性休克模型,四组均静脉泵入丙泊酚200μg/(kg·min)共10 min。在静脉泵入丙泊酚0、4、8、10、11、12、13、14、15、17.5、20、25、30、45、60、180 min时,测定丙泊酚血浆药物浓度。根据已测定的血药浓度计算丙泊酚的消除半衰期(t_(1/2))、血浆-效应室平衡速率常数(K_(e0))、药时曲线面积(AUC)及平均驻留时间(MRT)等药代动力学参数。结果丙泊酚血药浓度:D组与C组比较,差异有统计学意义(P〈0.05);DS组与CS组、D组比较,差异有统计学意义(P〈0.05)。最大药物浓度(C_(max)):D组高于C组,DS组高于CS组、D组,差异均有统计学意义(P〈0.05)。t_(1/2):D组较C组延长,DS组较CS组、D组延长,差异均有统计学意义(P〈0.05)。MRT:D组较C组延长,DS组较CS组、D组延长,差异均有统计学意义(P〈0.05)。K_(e0)、AUC:D组和CS组略高于C组,但差异无统计学意义(P〉0.05);DS组略高于D组、CS组,差异无统计学意义(P〉0.05)。结论低温环境下容量控制性失血性休克猪丙泊酚的药代动力学特点是代谢减慢,药物的消除半衰期延长,血浆-效应室平衡速率常数增大,体内平均驻留时间延长。 展开更多
关键词 低温 失血性休克 丙泊酚 药代动力学
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Liver transplantation with grafts obtained after cardiac death-current advances in mastering the challenge 预览
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作者 Fateh Bazerbachi Nazia Selzner +1 位作者 John B Seal Markus Selzner 《世界转化医学杂志》 2014年第2期58-68,共11页
The scarcity of donor livers has increased the interest in donation after cardiac death(DCD)as an additional pool to expand the availability of organs.However,the initial results of liver transplantation with DCD graf... The scarcity of donor livers has increased the interest in donation after cardiac death(DCD)as an additional pool to expand the availability of organs.However,the initial results of liver transplantation with DCD grafts have been suboptimal due to an increased rate of complications,as well as decreased graft survival.These challenges have led to many developments in DCD donation outcome,as well as basic and translational research.In this article we review the unique characteristics of DCD donors,nuances of DCD organ procurement,the effect of prolonged warm and cold ischemia times,and discuss major studies that compared DCD to donation after brain death liver transplantation,in terms of outcomes and complications.We also review the different methods of donor treatment that has been applied to ameliorate DCD organ outcome,and we discuss the role of machine perfusion techniques in organ reconditioning.We discuss the two major perfusion models,namely,hypothermic machine perfusion and normothermic machine perfusion;we compare both methods,and delineate their major differences. 展开更多
关键词 Transplant hepatology LIVER transplantation Organ DONATION Extended criteria donors DONATION AFTER cardiac death EX-VIVO LIVER PERFUSION Normothermic MACHINE PERFUSION Hypothermic MACHINE PERFUSION
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Effect of continuous hypothermic machine perfusion transport system(AirdriveTM) on canine kidney preservation
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《中华医学杂志:英文版》 SCIE CAS CSCD 2014年第6期1105-1109,共5页
Background Organ preservation keeps the quality of the organs under prolonged ischemia. Continuous machineperfusions are gaining an important position in clinical research and practice. The aim of this study was to ev... Background Organ preservation keeps the quality of the organs under prolonged ischemia. Continuous machineperfusions are gaining an important position in clinical research and practice. The aim of this study was to evaluate theprotective effect of continuous hypothermic machine perfusion transport system (AirdriveTM) on cold ischemic injury ofcanine kidney. 展开更多
关键词 CONTINUOUS hypothermic machine PERFUSION ORGAN PRESERVATION ORGAN transplantation CANINE KIDNEY common cold PRESERVATION
常温与低温体外循环下婴幼儿心脏直视手术对细胞因子的影响 预览
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作者 刘广文 范太兵 +1 位作者 陈红卫 潘砚鹏 《中国医药指南》 2013年第3期442-444,共3页
目的探讨常温及低温体外循环心脏直视手术对细胞因子的影响。方法方法选择先天性心脏病40例,随机分为常温组及低温组各20例,分别于术晨、体外循环结束时及术后6、12、24、48h抽取患者动脉血标本。测定血浆TNF-α、IL-6、IL-8。结果两组... 目的探讨常温及低温体外循环心脏直视手术对细胞因子的影响。方法方法选择先天性心脏病40例,随机分为常温组及低温组各20例,分别于术晨、体外循环结束时及术后6、12、24、48h抽取患者动脉血标本。测定血浆TNF-α、IL-6、IL-8。结果两组术前各项检查指标无显著差异。①常温体外循环下炎性因子升高水平低于中低温;②常温体外循环下炎性因子恢复时间比中低温明显缩短。结论常温体外循环心脏直视手术对细胞因子的影响显著轻于低温组,因而对术后机体的恢复优于低温方法。 展开更多
关键词 肿瘤坏死因子 体外循环 常温 低温
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常温和中低温体外循环对甲状腺素变化的影响 预览 被引量:1
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作者 刘广文 陈红卫 +1 位作者 潘砚鹏 范太兵 《中国心血管病研究杂志》 CAS 2012年第10期727-729,共3页
目的探讨常温及低温体外循环心脏直视手术对甲状腺素的影响。方法选择先天性心脏病患者80例,随机分为常温组及低温组,各40例,分别于术晨、体外循环结束时及术后6、12、24、48h抽取患者动脉血,测定血浆T3、T4和TSH。结果两组术前各... 目的探讨常温及低温体外循环心脏直视手术对甲状腺素的影响。方法选择先天性心脏病患者80例,随机分为常温组及低温组,各40例,分别于术晨、体外循环结束时及术后6、12、24、48h抽取患者动脉血,测定血浆T3、T4和TSH。结果两组术前各项检查指标差异无统计学意义。常温体外循环下甲状腺素T3升高水平低于中低温,恢复时间比中低温明显缩短。结论常温体外循环心脏直视手术对甲状腺素的影响显著轻于低温组,因而术后机体的恢复优于低温方法。 展开更多
关键词 体外循环 常温 低温 甲状腺素
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低温条件下兔肾脏血流动力学超声检测 预览 被引量:4
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作者 王宁 曹军英 张筠 《中国医学计算机成像杂志》 CSCD 北大核心 2012年第1期 72-75,共4页
目的:探讨低温条件下兔三级肾动脉血流动力学变化规律。方法:建立兔低温模型,选取20只兔,平均分为5组,分别置于室温,-10℃和-15℃中,分别于4h和8h时进行肾脏二维超声及彩色多普勒检查(CDFI)。二维超声测量肾脏大小,记录其长... 目的:探讨低温条件下兔三级肾动脉血流动力学变化规律。方法:建立兔低温模型,选取20只兔,平均分为5组,分别置于室温,-10℃和-15℃中,分别于4h和8h时进行肾脏二维超声及彩色多普勒检查(CDFI)。二维超声测量肾脏大小,记录其长、宽、厚径;多普勒流速曲线分析肾门部主肾动脉(MRA)、肾窦部段动脉(SRA)中部和肾锥体两侧缘的叶问动脉(IRA)的血流动力学指标:收缩期峰值血流速度(PSV/Vs),舒张末期最低血流速度(EDV/VD),阻力指数(RI)。结果:低温条件下。跨脏的各径线测值均无改变;血流参数收缩期峰值血流速度,舒张期最低血流速度和阻力指数在各级肾动脉表现出不同的特点。结论:超声检查可以反映低温条件下兔三级肾动脉的血流动力学改变,可为临床研究低温所引起的肾损害提供信息。 展开更多
关键词 低温 肾脏 彩色多普勒超声 血流动力学
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低温体外循环对罗库溴铵肌松效应的影响 预览 被引量:1
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作者 夏磊铭 姚尚龙 《医学新知杂志》 CAS 2011年第3期 175-177,共3页
目的利用加速度肌松监测法探讨低温体外循环对罗库溴铵肌松效应的影响。方法择期行心内直视术患者40例,ASAⅡ-Ⅲ级,随机分为2组:T组(n=20),自麻醉诱导至手术结束,采用TOF—Watch sx加速度肌松监测仪监测神经肌肉阻滞程度,并根据... 目的利用加速度肌松监测法探讨低温体外循环对罗库溴铵肌松效应的影响。方法择期行心内直视术患者40例,ASAⅡ-Ⅲ级,随机分为2组:T组(n=20),自麻醉诱导至手术结束,采用TOF—Watch sx加速度肌松监测仪监测神经肌肉阻滞程度,并根据TOF值追加罗库溴铵;C组(n=20)为对照组,除诱导期间采用TOF—Watch SX加速度肌松监仪指导气管插管外,手术过程中根据临床经验追加罗库溴铵。记录以下指标:①诱导前、静注插管剂量罗库溴铵前、后及插管后各时点患者平均动脉压(MAP)和心率(HR);②两组患者低温过程中罗库溴铵总用量、围手术期罗库溴铵总用量;③T组体外循环(CPB)前、低温过程中、复温后罗库溴铵维持时间。结果①两组病人插管时、插管后各时点平均动脉压(MAP)与诱导前相比无显著性(P〉0.05),心率(HR)与诱导前相比明显增快(P〈0.05或P〈0.01);②T组患者低温过程中罗库溴铵总用量明显少于C组,T组围术期罗库溴铵总用量也明显少于C组(P〈0.05);③T组低温过程中罗库溴铵维持时间明显长于降温前(P〈0.05),复温后罗库溴铵维持时间与降温前相比无差异性(P〉0.05)。结论低温体外循环中罗库溴铵用量明显减少,作用时间明显延长;罗库溴铵对心内直视手术病人血流动力学影响轻微;心内直视手术低温体外循环中应该对患者神经肌肉功能进行定量监测,便于指导肌松药的合理应用。 展开更多
关键词 低温 体外循环 罗库溴铵
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低温下筋膜内前列腺癌根治术对早期控尿及勃起功能的影响 被引量:2
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作者 乔勇 胡晓勇 李超 《临床泌尿外科杂志》 北大核心 2011年第9期669-671,共3页
目的:探讨低温下筋膜内前列腺癌根治术对早期控尿及勃起功能的影响。方法:选择穿刺活检证实的早期前列腺癌患者21例,其中有性生活者17例,在25℃生理盐水局部低温处理下行筋膜内前列腺癌根治术。结果:21例手术均顺利完成,出血量200... 目的:探讨低温下筋膜内前列腺癌根治术对早期控尿及勃起功能的影响。方法:选择穿刺活检证实的早期前列腺癌患者21例,其中有性生活者17例,在25℃生理盐水局部低温处理下行筋膜内前列腺癌根治术。结果:21例手术均顺利完成,出血量2001100(300±95)ml,平均留置尿管8(6~14)天。术后随访6个月,完全控尿18例(85%);17例术前有性生活的患者中,术后6个月可完成性生活者13例(76%)。结论:局部低温的应用减轻了前列腺癌根治术的创伤性炎症后遗反应;低温下筋膜内前列腺癌根治术对早期控尿与勃起功能的恢复有益。 展开更多
关键词 前列腺肿瘤 前列腺切除术 前列腺筋膜 低温
术中应用积极保温措施防止剖宫产产妇低体温寒颤 预览 被引量:11
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作者 陈少娟 郭雅梅 +2 位作者 赖梅 黄素娟 张莹 《广州医药》 2010年第5期 27-29,共3页
目的探讨术中应用积极的保温措施对防止剖宫产产妇低体温寒颤的影响。方法选取拟行剖宫产产妇100例,随机分为保温组和对照组,每组50例。对照组术中按传统护理常规进行护理,保温组采用多种积极的综合保温措施。结果低体温寒颤发生率对照... 目的探讨术中应用积极的保温措施对防止剖宫产产妇低体温寒颤的影响。方法选取拟行剖宫产产妇100例,随机分为保温组和对照组,每组50例。对照组术中按传统护理常规进行护理,保温组采用多种积极的综合保温措施。结果低体温寒颤发生率对照组为60%,保温组16%,两组比较差异有显著性(P〈0.05)。结论术中采取积极有效的保温措施有助于预防剖宫产产妇低体温寒颤的发生。 展开更多
关键词 保温 剖宫产 低体温 寒颤
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通气状态下离体肺保存的实验研究 预览
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作者 刘景亮 姜文航 +1 位作者 王传庆 王洪云 《临床肺科杂志》 2008年第10期 1257-1259,共3页
目的应用兔离体肺再灌注模型研究通气在离体肺保护中的作用。方法10只实验用兔20个单肺,分成A、B两组。A组为通气状态下低温再灌注组;B组为单纯低温再灌注组。分别在保存0、3、6.9、12、15、18h时取保存组织送组织学检查及引流灌注... 目的应用兔离体肺再灌注模型研究通气在离体肺保护中的作用。方法10只实验用兔20个单肺,分成A、B两组。A组为通气状态下低温再灌注组;B组为单纯低温再灌注组。分别在保存0、3、6.9、12、15、18h时取保存组织送组织学检查及引流灌注液测血气分析。结果在保存9h时A、B两组组织学结构发现差别,A组优于B组。血气分析示:A、B两组二氧化碳分压及PH无明显差别,氧分压有显著差别。结论在通气状态下低温灌注保存离体肺的效果明显优于单纯低温灌注保存方法。在通气状态下低温灌注保存离体肺的有效时限可达18h。 展开更多
关键词 通气 低温 肺灌洗肺保存
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Comparative study of the protective effect using hypothermic cardiopulmonary bypass and normothermic cardiopulmonary bypass 预览
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作者 HAN Pei - li FU Qing - lin +3 位作者 ZHANG Xin - zhong ZHANG Jie QIN Yuan - xu CUI Yu 《医学信息:手术学分册》 2007年第12期 1094-1099,共6页
Objective To explore the detrimental influence of normothermic and hypothermic cardiopulmonary bypass during open-heart surgery on immunity function,cytokines and complements.Methods Forty patients with congenital or ... Objective To explore the detrimental influence of normothermic and hypothermic cardiopulmonary bypass during open-heart surgery on immunity function,cytokines and complements.Methods Forty patients with congenital or rheumatic heart disease were randomized to receive the two strategies:normothermic CPB(study group)and hypothermic CPB(control group),20 cases in each group.Venous blood samples were collected at each time points of preoperation,end of CPB,day 1,4,7,14 postoperatively to examine the plasma level of IL-2,TNF-α,C3,C4,IgG,IgM,IgA,CD3,CD4,CD8.Results IL-2 in both groups decreased significantly at day 1,4,and returned to normal at day 7 postoperatively.IL-2 in control group was significantly lower than that in study group postoperatively.TNF-α in two groups was all higher at time points of end of CPB,day 1,4 postoperatively;in study group,it returned to normal level at day 7 postoperatively,whereas in control group,it was still higher at day 7 postoperatively than that before operation,and returned to normal at day 14 postoperatively.C3 in study group was significantly lower at time points of end of CPB,day 1,7 postoperatively than that in control group;C3 in both groups was all higher at time points of end of CPB,day 1,4 postoperatively;in study group,it returned to normal level at day 7 postoperatively,whereas in control group,it was still higher at day 7 postoperatively than that before operation and returned to normal at day 14 postoperatively.C4 in study group at time points of end of CPB,day 1 postoperatively was significantly lower than that in control group;C4 in both groups was all lower at time points of end of CPB,day 1,4 postoperatively than that before operation.The results showed that IgA after operation in both groups was significantly lower than that before operation,and returned to normal at day 7 postoperatively;IgA in study group at day 1 postoperatively was higher than that in control group.IgG in both group at time points of end of CPB,day 1,4 postoperatively was significantly lower 展开更多
关键词 心肺旁路手术 保护作用 手术治疗 体温
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低温低氧环境对心肌缺血家兔血浆内皮素和血液流变学的影响 预览 被引量:6
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作者 赵善民 何显教 +7 位作者 黄丽娟 黄俊杰 晋玲 梁祚仁 罗显克 覃志坚 罗章伟 黄彦峰 《中国心血管病研究杂志》 CAS 2004年第4期300-302,共3页
目的 探讨低温、低氧条件对正常家免与心肌缺血家兔内皮素(ET)和血液流变学的影响。方法 将家兔置于低温[(-10±2)℃]和低氧(混合气体中91.5%为氮,8.5%为氧)的冰柜中30min,然后测定血浆中ET和血液流变学的变化。结果 低温低... 目的 探讨低温、低氧条件对正常家免与心肌缺血家兔内皮素(ET)和血液流变学的影响。方法 将家兔置于低温[(-10±2)℃]和低氧(混合气体中91.5%为氮,8.5%为氧)的冰柜中30min,然后测定血浆中ET和血液流变学的变化。结果 低温低氧刺激组ET高于非低温低氧刺激组;心肌缺血组ET高于非低温低氧刺激组,也高于非心肌缺血组。同时,在低温低氧刺激下,正常家免血液流变学发生异常改变,心肌缺血家兔在低温低氧异常基础上出现加重的趋势。结论 低温低氧可使ET升高、血液流变学异常。提示:对于心肌缺血的患者,在低温低氧的环境中应加强保护。 展开更多
关键词 低温低氧环境 心肌缺血 家兔 血浆内皮素 血液流变学 ET 交感神经
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中低温停循环技术在复杂头颈部血管病变中的脑保护作用
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作者 张庆丰 董斌 +4 位作者 罗其中 江基尧 钟春龙 徐英辉 廉治刚 《中华实验外科杂志》 CAS CSCD 北大核心 2004年第10期 1249-1250,共2页
目的观察中低温停循环技术(MHCA)在复杂颈部大血管病变中的脑保护作用.方法制作大鼠永久性大脑中动脉(pMCAO)缺血模型,以代替一侧头颈外科中颈内动脉病变.建立大鼠闭胸式体外循环(CCFFB)中低温模型.实验分两组:对照组12只,37 ℃ pMCAO模... 目的观察中低温停循环技术(MHCA)在复杂颈部大血管病变中的脑保护作用.方法制作大鼠永久性大脑中动脉(pMCAO)缺血模型,以代替一侧头颈外科中颈内动脉病变.建立大鼠闭胸式体外循环(CCFFB)中低温模型.实验分两组:对照组12只,37 ℃ pMCAO模型,不予降温处理;实验组16只,2 h pMCAO模型制作后30 min内降温到26 ℃,停循环45、30 min内复温;温度监测以鼻咽温为准,复温后TTC染色观察梗死灶变化,计算梗死体积,分子生物学检测海马CA1组织凋亡基因bcl-2和bax的表达和光镜下观察海马组织神经元的形态学变化,TUNEL检测凋亡.结果对照组可以观察到严重的神经元损害,有大量凋亡细胞,TTC染色梗死和中心灶明显,实验组海马区可以观测到少量凋亡神经元细胞,神经元染色形态基本正常,梗死中心灶体积减小了 46.50%,周围灶的体积减小了 38.36% (P< 0.05).蛋白检测实验组bcl-2表达明显增加,bax表达无明显改变.结论采用MHCA技术有安全、较好的脑保护作用,有明显的抗神经元凋亡作用,可做为头颈外科复杂病变涉及颈部大血管手术时一个较好的辅助手段. 展开更多
关键词 头颈外科 低温 脑保护 脱噬作用
大鼠原位肝脏低温灌注和复流模型的建立及意义 被引量:12
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作者 胡建平 钱建民 +3 位作者 王学浩 陆森 孙倍成 张浩 《中华实验外科杂志》 CAS CSCD 北大核心 2001年第4期 366-367,共2页
目的 建立一种大鼠肝脏原位低温灌注再复流模型,用于离体及活体供肝肝切除术的研究。方法大鼠78只,分成4组,A、B组分别用乳酸林格氏液和自制灌注液灌注,C组仅行全肝血流阻断,D组作正常对照。脾静脉和右肾上腺静脉分别作为灌注液流... 目的 建立一种大鼠肝脏原位低温灌注再复流模型,用于离体及活体供肝肝切除术的研究。方法大鼠78只,分成4组,A、B组分别用乳酸林格氏液和自制灌注液灌注,C组仅行全肝血流阻断,D组作正常对照。脾静脉和右肾上腺静脉分别作为灌注液流入和流出道,行在体原位低温灌注,观察再复流后对肝细胞和肝窦内皮细胞的影响。结果 C组再复流2 h和6 h血清透明质酸浓度为(250.0±24.1)μg/L、(290.4±37.0)μg/L,分别高于A组(142.9±30.1)μg/L、(200.0±17.5)μg/L, 差异均有非常显著性(P<0.01);B组浓度为(63.3±16.2)μg/L、(62.1±14.6)μg/L,与A组相比浓度更低(P<0.01)。结论 该模型方法简单,易成功,可用于离体和活体供肝肝切除防止肝脏损伤的研究。 展开更多
关键词 SPRAGUE-DAWLEY 肝脏 低温灌注 肝切除 肝脏保护 建模
低温海水淹溺濒临死亡大鼠动物模型的研究 被引量:13
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作者 姜忠华 范静 +4 位作者 鲁青 王额尔敦 强琳 钟广文 苑同业 《中华劳动卫生职业病杂志》 CAS CSCD 北大核心 2001年第3期 201-204,共4页
目的建立低温海水淹溺濒临死亡动物模型,为开展相关研究奠定理论基础.方法将大鼠置于16℃~18℃低温海水中,自由游动,直至沉入水底;迅速取出动物,置室温下于不同时间(1、5、15、30及60min)观察生命指征,快速自左心室取血1ml行血气分析,... 目的建立低温海水淹溺濒临死亡动物模型,为开展相关研究奠定理论基础.方法将大鼠置于16℃~18℃低温海水中,自由游动,直至沉入水底;迅速取出动物,置室温下于不同时间(1、5、15、30及60min)观察生命指征,快速自左心室取血1ml行血气分析,处死动物,取左肺计算干湿重比值,透射电镜观察右肺超微结构.结果动物入海水后约15 min呼吸暂停;出海水后肛温、肺干湿重比值、呼吸频率和心率明显降低,1 min组呼吸频率达最低点(47±7)次/min,心率(70±14)次/min;5 min组肛温降至最低点(20.25±0.52)℃,上述指标除肺干湿重比值外均呈上升趋势,60min组呼吸频率达(111±26)次/min、心率达(177±28)次/min,接近正常对照组水平,差异无显著性(P>0.05),而肛温(23.82±0.72)℃和肺干湿重比值(0.08±0.03)仍未达正常对照组水平,差异有显著性(P<0.01).血pH、动脉氧分压(PaO2)及血氧饱和度(SaO2)明显降低,5 min组降至最低点[6.67±0.11、(5.91±2.98)kPa、43.30%±15.03%],随后呈上升趋势;60min组血PaO2(11.81±1.45)kPa及SaO2(88.63%±14.39%)接近正常对照组水平,差异无显著性(P>0.05),而血pH(7.10±0.14)仍未达正常对照组水平,差异有显著性(P<0.01).5 min及15 min组二氧化碳分压(PaCO2)明显升高,5min组升至最高点(13.18±1.88)kPa,差异有显著性(P<0.01),随后呈恢复趋势,30 min组[(8.72±2.72)kPa]及60min组[(8.91±1.54)kPa]接近正常对照组水平,差异无显著性(P>0.05).肺脏呈"湿肺”改变;12只动物出海水约15 min死亡.结论成功建立大鼠低温海水淹溺濒临死亡动物模型;动物死亡多发生在出海水15 min左右,主要死于低氧血症和酸中毒. 展开更多
关键词 动物模型 濒临淹溺死亡 低温 低氧血症 生命指征 血气分析 肺干湿重比值
解热静口服液药效学研究 预览
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作者 姚献花 刘霞 《中国实验方剂学杂志》 CAS 1997年第5期 16-19,共4页
解热静口服液对伤寒副伤寒、甲乙三联菌苗及酵母菌所致动物发热有较好的解热作用;可显著对抗二甲苯及蛋清引起的动物炎症反应;对戊巴比妥钠阈下催眠剂量有明显的协调催眠作用;并对临床常见致病菌有不同程度的抑制作用。
关键词 解热静口服液 药理学 中药品
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