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Harnessing the potential of gene editing technology using CRISPR in inflammatory bowel disease 预览
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作者 Viktor Limanskiy Arpita Vyas +1 位作者 Lakshmi Shankar Chaturvedi Dinesh Vyas 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第18期2177-2187,共11页
The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel ... The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel disease (IBD) and consequently decrease the 6.3 billion dollar annual financial healthcare burden in the treatment of IBD. For the past few years CRISPR technology has drastically revolutionized DNA engineering and biomedical research field. We are beginning to see its application in gene manipulation of sickle cell disease, human immunodeficiency virus resistant embryologic twin gene modification and IBD genes such as Gatm (Glycine amidinotransferase, mitochondrial), nucleotide-binding oligomerization domain-containing protein 2, KRT12 and other genes implicated in adaptive immune convergence pathways have been subjected to gene editing, however there are very few publications. Furthermore, since Crohn’s disease and ulcerative colitis have shared disease susceptibility and share genetic gene profile, it is paramount and is more advantageous to use CRISPR technology to maximize impact. Although, currently CRISPR does have its limitations due to limited number of specific Cas enzymes, off-target activity, protospacer adjacent motifs and crossfire between different target sites. However, these limitations have given researchers further insight on how to augment and manipulate enzymes to enable precise gene excision and limit crossfire between target sites. 展开更多
关键词 Clustered regularly interspersed short palindromic REPEATS INFLAMMATORY BOWEL DISEASE Crohn’s DISEASE Ulcerative colitis GENE excision GENE EDITING GENE therapy Financial impact of INFLAMMATORY BOWEL DISEASE on healthcare Clustered regularly interspersed short palindromic REPEATS crossfire
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Study on the relationship between nonresolving inflammation and tumor 预览
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作者 Jin-Feng Liu 《精准医学研究》 2019年第3期66-71,共6页
Due to persistent or low-intensity stimulation,tissue cells are in a state of injury for a long time,and the inflammatory state continues,eventually leading to nonresolving inflammation.In this nonresolving inflammati... Due to persistent or low-intensity stimulation,tissue cells are in a state of injury for a long time,and the inflammatory state continues,eventually leading to nonresolving inflammation.In this nonresolving inflammation,various inflammatory mediators and inflammatory cells act on tissue cells,and tumors are easily induced.Further,the unique microenvironment of the tumor further aggravates the development of uncontrolled inflammation.Eventually a vicious circle is formed.In this paper,we explored the mechanism of inflammatory mediators,inflammatory cells and tumors in nonresolving inflammation from the relationship between nonresolving inflammation and tumors,and provided some new ideas for the prevention and treatment of tumors. 展开更多
关键词 Nonresolving INFLAMMATION TUMOR INFLAMMATORY MEDIATOR INFLAMMATORY CELL
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三叶糖脂清DPP-4抑制成分筛选及其调控JNK/AKT信号通路改善胰岛素抵抗的机制研究 预览
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作者 石蔚华 赵筱萍 陈晓玲 《浙江中医药大学学报》 CAS 2019年第1期7-13,共7页
[目的]筛选三叶糖脂清中二肽基肽酶-4(dipeptidyl peptidase-4,DPP-4)的抑制成分,探讨三叶糖脂清及其DPP-4抑制成分对胰岛素抵抗(insulin resistant,IR)细胞的影响和作用机制。[方法]运用课题组前期建立的DPP-4抑制成分筛选方法,从三叶... [目的]筛选三叶糖脂清中二肽基肽酶-4(dipeptidyl peptidase-4,DPP-4)的抑制成分,探讨三叶糖脂清及其DPP-4抑制成分对胰岛素抵抗(insulin resistant,IR)细胞的影响和作用机制。[方法]运用课题组前期建立的DPP-4抑制成分筛选方法,从三叶糖脂清中筛选DPP-4抑制成分,并对抑制率大于50%的化合物进行量效考察,通过高浓度胰岛素诱导BNL.CL2小鼠胚胎肝细胞构建IR细胞模型。通过检测葡萄糖消耗量、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)水平和锰超氧化物歧化酶(manganese superoxide dismutase,Mn-SOD)含量,考察三叶糖脂清总提取物及其DPP-4抑制成分对IR的BNL.CL2细胞的影响。以Western blot检测超氧化物歧化酶2(superoxide dismutase 2,SOD2)、磷酸化c-Jun氨基末端激酶(phospho-c-Jun N-terminal kinase,p-JNK)、c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)、磷酸化蛋白激酶B(phospho-protein kinase B,p-AKT/PKB)、蛋白激酶B(protein kinase B,AKT/PKB)的蛋白表达。[结果]三叶糖脂清中筛选出6个化合物对DPP-4的抑制率大于50%,且在10~50μmol·L-1浓度范围呈良好的量效关系。三叶糖脂清总提取物及其DPP-4抑制成分隐丹参酮能促进BNL.CL2细胞葡萄糖消耗和Mn-SOD的表达,抑制IL-1β、IL-6的分泌,降低JNK蛋白磷酸化,促进AKT蛋白磷酸化和SOD2蛋白表达。[结论]三叶糖脂清中含有DPP-4抑制作用的成分,三叶糖脂清总提取物及其成分隐丹参酮可能通过降低氧化应激和炎症反应水平来调节JNK/AKT通路,改善IR。 展开更多
关键词 三叶糖脂清 DPP-4 胰岛素抵抗 氧化应激 炎症 JNK
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Effects of adjuvant raw rhubarb enema therapy on systemic inflammatory stress response and intestinal mucosal barrier function in patients with severe pancreatitis
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作者 Ping Fang Xian-Hua Xiao 《海南医科大学学报(英文版)》 2019年第9期50-53,共4页
Objective: To investigate the effects of adjuvant raw rhubarb enema therapy on systemic inflammatory stress response and intestinal mucosal barrier function in patients with severe pancreatitis. Methods: A total of 78... Objective: To investigate the effects of adjuvant raw rhubarb enema therapy on systemic inflammatory stress response and intestinal mucosal barrier function in patients with severe pancreatitis. Methods: A total of 78 patients with severe pancreatitis treated in the Second People's Hospital of Neijiang, Sichuan, China between December 2016 and September 2018 were included in the study. The patients were divided into control group (n=39) and raw rhubarb group (n=39) by simple randomization. The control group of patients received conventional treatment of severe pancreatitis with western medicine, while the raw rhubarb group of patients received raw rhubarb enema on the basis of the treatment of control group, and the efficacy was evaluated after continuous treatment for 1 week. The differences in serum levels of inflammatory mediators (high mobility group protein B1, C-reactive protein, prostaglandin E2 and tumor necrosis factor α), stress hormones (cortisol and epinephrine) as well as intestinal mucosal barrier function indicators (D-lactate, endotoxin and diamine oxidase) were compared between the two groups of patients before and after treatment. Results: Before treatment, there was no statistically significant difference in serum levels of inflammatory mediators, stress hormones and intestinal mucosal barrier function indicators between the two groups (P>0.05). After treatment, serum high mobility group protein B1, C-reactive protein, prostaglandin E2 and tumor necrosis factor levels of the observation group were significantly lower than those of the control group;cortisol and epinephrine levels were significantly lower than those of the control group;D-lactate, endotoxin and diamine oxidase levels were significantly lower than those of the control group (P<0.05). Conclusions:Adjuvant raw rhubarb enema therapy on the basis of western medicine can help alleviate the inflammatory stress response and optimize the intestinal mucosal barrier function in patients with severe pancreatitis. 展开更多
关键词 SEVERE PANCREATITIS RAW RHUBARB enema SYSTEMIC INFLAMMATORY response Stress response Intestinal mucosal barrier function
Atsttrin reduces lipopolysaccharide-induced neuroinflammation by inhibiting the nuclear factor kappa B signaling pathway 预览
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作者 Lian Liu Yuan Qu +7 位作者 Yi Liu Hua Zhao He-Cheng Ma Ahmed Fayyaz Noor Chang-Jiao Ji Lin Nie Meng Si Lei Cheng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1994-2002,共9页
Progranulin is closely related to neuronal survival in a neuroinflammatory mouse model and attenuates inflammatory reactions. Atsttrin is an engineered protein composed of three progranulin fragments and has been show... Progranulin is closely related to neuronal survival in a neuroinflammatory mouse model and attenuates inflammatory reactions. Atsttrin is an engineered protein composed of three progranulin fragments and has been shown to have an effect similar to that of progranulin. Atsttrin has anti-inflammatory actions in multiple arthritis mouse models, and it protects against further arthritis development. However, whether Atsttrin has a role in neuroinflammation remains to be elucidated. In this study, we produced a neuroinflammatory mouse model by intracerebroventricular injection of 1 μL lipopolysaccharide(10 μg/μL). Atsttrin(2.5 mg/kg) was administered via intraperitoneal injection every 3 days over a period of 7 days before intracerebroventricular injection of 1 μL lipopolysaccharide(10 μg/μL). In addition, astrocyte cultures were treated with 0, 100 or 300 ng/mL lipopolysaccharide, with 200 ng/mL Atsttrin simultaneously. Immunohistochemistry, enzyme-linked immunosorbent assay and real-time reverse transcription-polymerase chain reaction were performed to examine the protein and mRNA levels of inflammatory mediators and to assess activation of the nuclear factor kappa B signaling pathway. Progranulin expression in the brain of wild-type mice and in astrocyte cultures was increased after lipopolysaccharide administration. The protein and mRNA expression levels of tumor necrosis factor-α, interleukin-1β and inducible nitric oxide synthase were increased in the brain of progranulin knockout mice after lipopolysaccharide administration. Atsttrin treatment reduced the lipopolysaccharide-induced increase in the protein and mRNA levels of tumor necrosis factor-α, interleukin-1β, matrix metalloproteinase-3 and inducible nitric oxide synthase in the brain of progranulin knockout mice. Atsttrin also reduced the expression of cyclooxygenase-2, inducible nitric oxide synthase and matrix metalloproteinase 3 mRNA in lipopolysaccharide-treated astrocytes in vitro, and decreased the concentration of tumor necrosis factor α 展开更多
关键词 nerve REGENERATION progranulin Atsttrin NEUROINFLAMMATION inflammatory cytokines LIPOPOLYSACCHARIDE INTRACEREBROVENTRICULAR injection ASTROCYTE nuclear factor kappa B signaling pathway progranulin knockout mouse CEREBROSPINAL fluid neural REGENERATION
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New ex vivo demyelination/ remyelination models to defeat multiple sclerosis and neuromyelitis optica 预览
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作者 Yiting Liu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1715-1716,共2页
Multiple sclerosis (MS) and neuromyelitis optica (NMO) are inflammatory diseases of the central nervous system (CNS) resulting in CNS inflammation, infiltration of peripheral immune cells, loss of myelin and oligodend... Multiple sclerosis (MS) and neuromyelitis optica (NMO) are inflammatory diseases of the central nervous system (CNS) resulting in CNS inflammation, infiltration of peripheral immune cells, loss of myelin and oligodendrocytes, interruption of axonal communication, and neurologic deficits. Following oligodendrocyte injury, newly generated myelinating oligodendrocytes derived from oligodendrocyte progenitors (OPCs) may produce new myelin sheaths around denuded axons (remyelination) restoring neuronal function (Verden and Macklin, 2016). While remyelination is apparent in MS lesions, the process is often inefficient;in NMO, remyelination is even more limited. 展开更多
关键词 Multiple sclerosis(MS) inflammatory diseases REMYELINATION is EVEN more LIMITED
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Effects of adjuvant Qishen Yiqi Drop Pill therapy on renal function changes and prognosis of patients with early diabetic nephropathy
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作者 Jun Li Xiao-Hong Zhang 《海南医科大学学报(英文版)》 2019年第3期31-35,共5页
Objective: To study the effects of adjuvant Qishen Yiqi Drop Pill therapy on renal function changes and prognosis of patients with early diabetic nephropathy. Methods: The patients with early diabetic nephropathy trea... Objective: To study the effects of adjuvant Qishen Yiqi Drop Pill therapy on renal function changes and prognosis of patients with early diabetic nephropathy. Methods: The patients with early diabetic nephropathy treated in our hospital between February 2015 and April 2017 were chosen and divided into two groups by random number table, observation group received Qishen Yiqi Drop Pill combined with conventional therapy and control group accepted conventional therapy. The renal function indexes, cytokine contents and oxygen free radical generation were compared before treatment and 3 months after treatment. Results:Urine UREA levels as well as serum β2 microglobulin (β2-MG), cystatin C (CysC), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), stromal cell-derived factor-1 (SDF-1), interleukin-17 (IL-17), transforming growth factor-β1 (TGF-β1), malondialdehyde (MDA) and 8-iso-prostaglandin F2α (8-iso-PGF2α) contents of both groups were significantly lower while superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) contents were significantly higher after treatment, and urine UREA level as well as serum β2-MG, CysC, TNF-α, IL-6, SDF-1, IL-17, TGF-β1, MDA and 8-iso-PGF2α contents of observation group after treatment was significantly lower than those of control group while SOD and T-AOC contents were significantly higher than those of control group. Conclusion: Adjuvant Qishen Yiqi Drop Pill therapy can improve the renal function and reduce the inflammatory response and oxidative stress response in early diabetic nephropathy. 展开更多
关键词 DIABETIC NEPHROPATHY Qishen Yiqi DROP PILL Renal function Inflammatory RESPONSE Oxidative stress RESPONSE
Effects of Liraglutide on endothelial function, immune function and related factors in patients with type 2 diabetes mellitus
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作者 De-Zhi Bian Chun-Lei Wang +1 位作者 Ke Xu Yan-Mei Liu 《海南医科大学学报(英文版)》 2019年第3期27-30,共4页
Objective: To investigate the effects of Liraglutide on endothelial function, immune function and related factors in patients with type 2 diabetes mellitus. Methods: A total of 160 patients with type 2 diabetes mellit... Objective: To investigate the effects of Liraglutide on endothelial function, immune function and related factors in patients with type 2 diabetes mellitus. Methods: A total of 160 patients with type 2 diabetes mellitus who received treatment in department of endocrinology of our hospital from December 2015 to June 2018 were collected. They were randomly divided into the control group and the observation group, 80 cases in each group. The patients in the control group were treated with metformin. The patients in the observation group were treated with liraglutide on the basis of the control group. The levels of endothelin (ET-1), nitric oxide (NO), immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin G (IgG), hypersensitive C-reactive protein (hs-CRP), insulin-like growth factor-1 (IGF-1), tumor necrosis factor- (TNF-α), Vaspin and HbAlc were detected and compared. Results: After treatment, compared with the treatment group, the serum levels of ET-1, hs-CRP, IGF-1, TNF-α and HbAlc of patients in the control group and the observation group were significantly decreased, and the levels of NO, IgA, IgG, IgM and Vaspin increased significantly. The change trend of the above indexes of patients in the observation group was more obvious than those in the control group (P<0.05). Conclusion: Liraglutide combined with metformin in the treatment of type 2 diabetes mellitus can significantly enhance endothelial function and immune function of patients, and alleviate inflammation, and it has good clinical efficacy. 展开更多
关键词 LIRAGLUTIDE METFORMIN Type 2 diabetes MELLITUS Endothelial FUNCTION Immune FUNCTION Inflammatory factors
痰热清注射液联合左氧氟沙星对老年肺炎患者红细胞分布宽度及炎症标志物的影响 预览
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作者 陶忠义 江畅 许佑冬 《中国医院用药评价与分析》 2019年第9期1101-1103,共3页
目的:探讨痰热清注射液联合左氧氟沙星对老年肺炎患者红细胞分布宽度(RDW)及炎症标志物的影响。方法:回顾性选取2017年3月至2018年12月安徽医科大学附属六安医院收治的老年肺炎患者157例,其中采用痰热清注射液联合左氧氟沙星注射液治疗... 目的:探讨痰热清注射液联合左氧氟沙星对老年肺炎患者红细胞分布宽度(RDW)及炎症标志物的影响。方法:回顾性选取2017年3月至2018年12月安徽医科大学附属六安医院收治的老年肺炎患者157例,其中采用痰热清注射液联合左氧氟沙星注射液治疗的87例患者设为观察组,单独采用左氧氟沙星注射液治疗的70例患者作为对照组。观察两组患者的临床疗效和不良反应发生情况,比较两组患者的RDW、白细胞计数(WBC)、中性粒细胞绝对值(N)、C反应蛋白(CRP)、血沉(ESR)、降钙素原(PCT)和纤维蛋白原(FIB)水平的差异,判断治疗前后RDW及炎症标志物的变化趋势,分析RDW与炎症指标的相关性。结果:观察组患者的总有效率为95.40%(83/87),明显高于对照组的75.71%(53/70),差异有统计学意义(P<0.05)。治疗前,两组患者RDW、WBC、N、CRP、ESR、PCT和FIB水平的差异均无统计学意义(P>0.05);治疗后,观察组患者RDW、WBC、N、CRP、ESR、PCT和FIB水平明显低于对照组,差异均有统计学意义(P<0.05)。157例患者治疗前RDW水平与WBC、N、CRP、ESR、PCT和FIB水平有显著正相关性,治疗后RDW水平与WBC、N、CRP、ESR、PCT和FIB水平无相关性。观察组、对照组患者不良反应发生率分别为5.75%(5/87)、8.57%(6/70),差异无统计学意义(P>0.05)。结论:痰热清注射液用于老年肺炎患者具有良好的抗炎作用,RDW水平与炎症活动呈正相关性。 展开更多
关键词 肺炎 红细胞分布宽度 炎症 相关性
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姜黄素通过TLR4-MAPK/NF-κB信号通路对巨噬细胞极化及炎症反应的影响 预览
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作者 唐彪 周瑶瑶 《心电与循环》 2019年第5期389-394,共6页
目的探讨姜黄素对巨噬细胞极化及表达炎症因子的影响并研究相关的分子机制。方法采用脂多糖诱导巨噬细胞向M1型极化并设立不同浓度的姜黄素干预组和对照组,通过实时荧光定量PCR及酶联免疫吸附法测定各组肿瘤坏死因子α(TNF-α)及白细胞... 目的探讨姜黄素对巨噬细胞极化及表达炎症因子的影响并研究相关的分子机制。方法采用脂多糖诱导巨噬细胞向M1型极化并设立不同浓度的姜黄素干预组和对照组,通过实时荧光定量PCR及酶联免疫吸附法测定各组肿瘤坏死因子α(TNF-α)及白细胞介素6(IL-6)的表达。通过siTLR4、JNK的抑制剂SP600125、ERK的抑制剂SCH772984以及p38的抑制剂Skepinone-L在不同水平阻断信号通路,Westernblot法检测各组细胞TLR4-MAPK/NF-κB信号通路p38/ERK/JNK/IKK/IκBα/p65的总体水平及磷酸化水平。结果姜黄素抑制巨噬细胞向M1型极化及炎症因子TNF-α及IL-6的表达;抑制LPS引起的TLR4-MAPK/NF-κB通路的激活,具体表现为p38/ERK/JNK/IκBα/p65的磷酸化水平的回落(P<0.05)。通过SP600125、SCH772984以及Skepinone-L分别在不同水平阻断TLR4/MAPK通路的传导,进一步证实TLR4/MAPK信号通路可影响M1型细胞因子TNF-α和IL-6的表达(P<0.05)。结论姜黄素通过抑制TLR4-MAPK/NF-κB信号通路抑制巨噬细胞向M1型极化并减少炎症因子TNF-α及IL-6的表达。 展开更多
关键词 姜黄素 巨噬细胞 极化 炎症 分子机制
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白细胞介素-38的炎症免疫作用及调控机制研究进展 预览
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作者 柴雨森 王川江 +1 位作者 林时辉 徐昉 《中国免疫学杂志》 CAS CSCD 北大核心 2019年第2期230-235,共6页
白细胞介素(IL)-38是IL-1家族新成员,其基因序列与IL-1受体拮抗剂(IL-1Ra)和IL-36Ra的基因有较高同源性,呈现IL-36部分受体拮抗剂的功能。目前认为IL-38可广泛参与胞内感染、炎症反应等体内免疫应答,在自身免疫病、骨关节炎、肿瘤、心... 白细胞介素(IL)-38是IL-1家族新成员,其基因序列与IL-1受体拮抗剂(IL-1Ra)和IL-36Ra的基因有较高同源性,呈现IL-36部分受体拮抗剂的功能。目前认为IL-38可广泛参与胞内感染、炎症反应等体内免疫应答,在自身免疫病、骨关节炎、肿瘤、心肺疾病等疾病中起重要调节作用。然而,IL-38的分子免疫调控机制复杂,涉及特异性受体募集、凋亡介导的吞噬细胞调控、调控Th17细胞分化等诸多过程,这也为疾病治疗提供了多条新的线索。IL-38已成为多种疾病生物治疗研究的新热点,具有广泛的应用前景和深入探索的价值。 展开更多
关键词 白细胞介素-38 细胞因子 炎症 免疫
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KOA患者关节镜有限清理术后临床症状和炎症指标变化及补肾壮骨汤的改善作用
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作者 朱康 王晓桐 +2 位作者 马明 马胡晶 丁宝江 《时珍国医国药》 CAS CSCD 北大核心 2019年第8期1920-1922,共3页
目的分析膝骨性关节炎(KOA)患者关节镜有限清理术后临床症状和炎症指标变化及补肾壮骨汤的改善作用。方法选取86例KOA患者,随机分为对照组采取关节镜有限清理术,观察组在对照组治疗基础上应用补肾壮骨汤治疗,两组各43例。比较两组患者... 目的分析膝骨性关节炎(KOA)患者关节镜有限清理术后临床症状和炎症指标变化及补肾壮骨汤的改善作用。方法选取86例KOA患者,随机分为对照组采取关节镜有限清理术,观察组在对照组治疗基础上应用补肾壮骨汤治疗,两组各43例。比较两组患者临床疗效、治疗前后临床症状评分和血清细胞因子水平的变化情况。结果相比对照组,观察组治疗总有效率显著升高(P<0.05)。相比治疗前,两组患者治疗后关节活动度评分、膝关节疼痛评分、晨僵评分和总评分均明显减少(P<0.05);相比对照组,观察组治疗后各项临床症状评分和总评分均明显减少(P<0.05)。与治疗前比较,两组患者治疗后血清转化生长因子-1(TGF-1)含量显著增高,白细胞介素-1(IL-1)含量显著下降(P<0.05);与对照组比较,观察组治疗后血清TGF-1含量显著升高,IL-1含量显著降低(P<0.05)。结论在关节镜有限清理术治疗的同时,应用补肾壮骨汤治疗不仅可有效减轻KOA患者晨僵和膝关节疼痛,提高关节活动度,改善膝关节功能,临床疗效确切,而且可有效改善机体炎症指标水平,减轻体内炎症程度,因此具有良好的临床应用价值。 展开更多
关键词 膝骨性关节炎 补肾壮骨汤 关节镜有限清理术 临床症状 炎症
早产的发生机制及预测因子新进展 预览
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作者 付锦娴 陈友鹏 陈新 《暨南大学学报:自然科学与医学版》 CAS CSCD 北大核心 2019年第4期334-338,共5页
早产是导致围生儿死亡及新生儿疾病的重要因素,是临床工作中频繁出现的挑战与难题.影响早产的因素众多,生理或心理因素、先天或后天因素都可能在妊娠过程中引致早产.早产的机制更复杂,可能与炎症机制、内分泌系统的调节、母体基因背景... 早产是导致围生儿死亡及新生儿疾病的重要因素,是临床工作中频繁出现的挑战与难题.影响早产的因素众多,生理或心理因素、先天或后天因素都可能在妊娠过程中引致早产.早产的机制更复杂,可能与炎症机制、内分泌系统的调节、母体基因背景等有关.目前临床上预测早产的手段较为局限,综合早产的病因机制及预测因子,查找更多的早产预测因子,为早产的诊断与防治提供依据. 展开更多
关键词 早产 免疫失衡 炎症 蜕膜早衰 预测因子
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TSG-6蛋白在疾病模型中的研究进展
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作者 侯林林 高艳霞 《临床急诊杂志》 CAS 2019年第9期696-700,共5页
肿瘤坏死因子α刺激基因-6(tumor necrosis factor a stimulated gene 6,TSG-6)蛋白是一种35 kd的炎症诱导蛋白,最初是通过对TNF刺激的人FS-4成纤维细胞制备的cDNA文库进行鉴别筛选而发现的,位于人染色体2q23.3,mRNA全长1440 bp,其启动... 肿瘤坏死因子α刺激基因-6(tumor necrosis factor a stimulated gene 6,TSG-6)蛋白是一种35 kd的炎症诱导蛋白,最初是通过对TNF刺激的人FS-4成纤维细胞制备的cDNA文库进行鉴别筛选而发现的,位于人染色体2q23.3,mRNA全长1440 bp,其启动子序列中存在白细胞介素(interleukin,IL)和核因子的结合位点。 展开更多
关键词 TSG-6蛋白 透明质酸 炎症反应 间充质干细胞
Protective Effect of Bacillus subtilis Peptidoglycan (PG) on β-conglycinin-induced Intestinal Epithelial Cells Damage of Juvenile Carp (Cyprinus carpio) 预览
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作者 Zhang Tiantian Yin Haicheng Huang Wei 《动物与饲料科学:英文版》 CAS 2019年第2期56-61,共6页
[Objective] The paper was to investigate the protective effects of different concentrations of Bacillus subtilis peptidoglycan(PG) on β-conglycinin-induced inflammatory injury in intestinal epithelial cells of juveni... [Objective] The paper was to investigate the protective effects of different concentrations of Bacillus subtilis peptidoglycan(PG) on β-conglycinin-induced inflammatory injury in intestinal epithelial cells of juvenile carp(Cyprinus carpio).[Method] In 24-cell microplates, the intestinal epithelial cells(IECs) of juvenile carp were primarily cultured for 72 h at 26°C and 6% CO2, and then the IECs were randomly divided into6 groups with 4 replicates per group. One of the six groups was set as negative control group, and the other groups were all supplemented with 1.0 mg/mL β-conglycinin in culture medium to establish inflammatory injury. At 24 h post induction, the culture media were changed into B. subtilis PG culture media with the concentrations of 0(positive control group), 0.15, 0.30, 0.45 and 0.60 mg/mL, respectively. The samples were collected to measure the antioxidant and anti-inflammatory indices at 12, 24 and 36 h post culture.[Result]β-conglycinin exposure significantly decreased the activity of ASA, AHR, SOD, CAT, GPx, and increased the PC content and the mRNA expression of inflammatory cytokines(IL-1β, IL-8, TNF-α1,IL-10 and TGF-β). At 12, 24 and 36 h post PG treatment, the activities of ASA, AHR, SOD, CAT, GPx and the content of PC in cells decreased in a dose-dependent manner;the mRNA levels of IL-1β, IL-8 and TNF-α1 were down-regulated and those of IL-10 and TGF-β were up-regulated.[Conclusion] Different concentrations of B. subtilis PG could protect IECs oxidative damage induced by β-conglycinin and improve the antioxidant capacity of IECs. High concentration of PG could improve the anti-inflammatory ability of IECs by inhibiting inflammatory factors and promoting the gene expression of anti-inflammatory cytokines. 展开更多
关键词 CARP (Cyprinus carpio) Bacillus subtilis PG Β-CONGLYCININ Intestinal epithelial cell (IECs) Antioxidant Inflammatory factor
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Characterization of inflammatory factor-induced changes in mesenchymal stem cell exosomes and sequencing analysis of exosomal microRNAs 预览
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作者 Chen Huang Wen-Feng Luo +8 位作者 Yu-Feng Ye Li Lin Zhe Wang Ming-Hua Luo Qi-De Song Xue-Ping He Han-Wei Chen Yi Kong Yu-Kuan Tang 《世界干细胞杂志:英文版(电子版)》 2019年第10期859-890,共32页
BACKGROUND Treatments utilizing stems cells often require stem cells to be exposed to inflammatory environments,but the effects of such environments are unknown.AIM To examine the effects of inflammatory cytokines on ... BACKGROUND Treatments utilizing stems cells often require stem cells to be exposed to inflammatory environments,but the effects of such environments are unknown.AIM To examine the effects of inflammatory cytokines on the morphology and quantity of mesenchymal stem cell exosomes(MSCs-exo)as well as the differential expression of microRNAs(miRNAs)in the exosomes.METHODS MSCs were isolated from human umbilical tissue by enzymatic digestion.Exosomes were then collected after a 48-h incubation period in a serum-free medium with one of the following the inflammatory cytokines:None(control),vascular cell adhesion molecule-1(VCAM-1),tumor necrosis factor(TNF)α,and interleukin(IL)6.The morphology and quantity of each group of MSC exosomes were observed and measured.The miRNAs in MSCs-exo were sequenced.We compared the sequenced data with the miRBase and other non-coding databases in order to detect differentially expressed miRNAs and explore their target genes and regulatory mechanisms.In vitro tube formation assays and Western blot were performed in endothelial cells which were used to assess the angiogenic potential of MSCs-exo after inflammatory cytokine stimulation.RESULTS MSCs-exo were numerous,small,and regularly shaped in the VCAM-1 group.TNFαstimulated MSCs to secrete larger and irregular exosomes.IL6 led to a reduced quantity of MSCs-exo.Compared to the control group,the TNFαand IL6 groups had more downregulated differentially expressed miRNAs,particularly angiogenesis-related miRNAs.The angiogenic potential of MSCs-exo declined after IL6 stimulation.CONCLUSION TNFαand IL6 may influence the expression of miRNAs that down-regulate the PI3K-AKT,MAPK,and VEGF signaling pathways;particularly,IL6 significantly down-regulates the PI3K-AKT signaling pathway.Overall,inflammatory cytokines may lead to changes in exosomal miRNAs that abnormally impact cellular components,molecular function,and biological processes. 展开更多
关键词 MESENCHYMAL stem cells EXOSOMES MiRNA INFLAMMATORY CYTOKINES ANGIOGENESIS
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Data mining in Xu Runsan's Traditional Chinese Medicine practice:treatment of chronic pelvic pain caused by pelvic inflammatory disease
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作者 Liu Liuqing Yang Fang +1 位作者 Xin Ling Jing Yan 《中医杂志:英文版》 SCIE CAS CSCD 2019年第3期440-450,共11页
OBJECTIVE:To research the Traditional Chinese Medicine(TCM)practice of Professor Xu Runsan for treatment of chronic pelvic pain(CPP)caused by sequelae of pelvic inflammatory disease(SPID)by data mining.METHODS:The med... OBJECTIVE:To research the Traditional Chinese Medicine(TCM)practice of Professor Xu Runsan for treatment of chronic pelvic pain(CPP)caused by sequelae of pelvic inflammatory disease(SPID)by data mining.METHODS:The medical records of inpatients at China-Japan Friendship Hospital confirmed to have CPP caused by SPID were collected(274 visits in total).The data extracted from the medical records were analyzed by frequency statistics,correlation analyses,cluster analyses,and complex network analyses.RESULTS:The most frequently used medicines were warm medicines,bitter medicines,and medicines distributed to the liver meridian.The most common medicinal combinations were Chishao(Radix Paeoniae Rubra)plus Huangqi(Radix Astragali Mongolici)plus Sanqi(Radix Notoginseng)and Ezhu(Rhizoma Curcumae Phaeocoulis);Guizhi(Ramulus Cinnamomi)plus Fuling(Poria)and Chishao(Radix Poeoniae Rubra);and Chaihu(Radix Bupleuri Chinensis)plus Zhishi(Fructus Aurantii Immaturus)and Gancao(Radix Glycyrrhizae).The most frequently used medicines were divided into four groups according to their efficacy;i.e.,medicines that could(a)warm meridians and free collateral vessels,(b)regulate Qi and free collateral vessels,(c)fortify the spleen and nourish blood and Qi,and(d)tonify Qi and activate blood.The most commonly used formulations were Guizhi FulingPill and Sini Powder.The core medicines extracted based on complex network analyses were Chishao(Radix Poeoniae Rubra),Sanqi(Radix Notoginseng),Hua ngqi(Radix Astragali Mongolica),Danshen(Radix Salviae Miltiorrhizae),Ezhu(Rhizoma Curcumae Phaeocaulis), Gancao(Radix Glycyrrhizae),Chaihu(Radix Bupleuri Chinensis),Guizhi(Romulus Cinnomomi),Shuizhi(Hirudo), Fuling(Poria),and Zhishi(Fructus Aurantii Immaturus).CONCLUSION:According to the TCM practice of Professor Xui,treatment of CPP caused by SPID should focus on dissolving stasis and obstructions using medicines that can activate blood,resolve stasis,regulate Qi,and dissipate adhesions.His prescriptions are often based on Guizhi Fuling Pill and Sini Powder. 展开更多
关键词 PELVIC PAIN PELVIC inflammatory disease Drugs Chinese HERBAL Cluster ANALYSIS Complex network ANALYSIS
Sexual health and fertility for individuals with inflammatory bowel disease 预览
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作者 Romain Leenhardt Pauline Rivière +4 位作者 Patrick Papazian Isabelle Nion-Larmurier Guillaume Girard David Laharie Philippe Marteau 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第36期5423-5433,共11页
The impact of a chronic disease such as inflammatory bowel disease (IBD) on sexual functioning and body image can significantly impair the quality of life of patients. This review considers the sexual and fertility as... The impact of a chronic disease such as inflammatory bowel disease (IBD) on sexual functioning and body image can significantly impair the quality of life of patients. This review considers the sexual and fertility aspects of IBD patients and their daily management. Modern IBD healthcare management should include appropriate communication on sexuality and consider psychological, physiological, and biological issues. Patients with IBD have less children than the general population, and voluntary childlessness is frequent. The most influential factors reported by IBD patients who experience fertility alteration are psychological and surgery-related problems. Pregnancy is a major concern for patients, and any pregnancy for IBD patients should be closely followed-up to keep the chronic disease in a quiescent state. Preconceptional consultation is of great help. 展开更多
关键词 Inflammatory BOWEL DISEASE Crohn’s DISEASE ULCERATIVE COLITIS FERTILITY Pregnancy SEXUALITY
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超声影像技术在炎性关节病变诊疗中的应用研究 预览
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作者 李红琼 《影像研究与医学应用》 2019年第11期98-99,共2页
目的:探究分析超声影像技术在炎性关节病变当中的应用价值以及诊断效果。方法:选取我院在2016年—2018年期间我院所收治的共计100名关节炎性病变的患者作为研究对象,将所有的患者按照临床上的诊断方式不同分为对照组和观察组。其中对照... 目的:探究分析超声影像技术在炎性关节病变当中的应用价值以及诊断效果。方法:选取我院在2016年—2018年期间我院所收治的共计100名关节炎性病变的患者作为研究对象,将所有的患者按照临床上的诊断方式不同分为对照组和观察组。其中对照组人数共计50名实施X射线诊断方式进行,观察组人数共计50名实施超声诊断方式。对比两组患者在临床上的诊断准确率情况。结果:通过对比分析来看,观察组患者实施超声诊断方式后,医生能够更加清楚的对患者关节周围软组织异常情况进行分析,发现局部所存在的活动性炎症,同时有效的对患者肌肉骨骼显示,相比于X射线检查诊断准确率方面显著提升,差异具有统计学意义(P <0.05)。结论:在当前阶段针对炎性关节炎来说,通过实施正确的超声诊断方式能够有效的提升实际的诊断效果,对于患者的诊断准确率具有积极的意义和作用,可在临床上进行推广。 展开更多
关键词 超声诊断 炎性 关节炎
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Probiotic Mixture VSL#3 Alleviates Dextran Sulfate Sodium-induced Colitis in Mice by Downregulating T Follicular Helper Cells 预览
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作者 Xiao-jing LIU Ran YU Kai-fang ZOU 《当代医学科学(英文)》 SCIE CAS 2019年第3期371-378,共8页
Clinical trials have shown beneficial effects of probiotics on inflammatory bowel diseases (IBD), although the exact mechanism remains unknown. VSL#3, a mixture of 8 probiotic bacteria, has been confirmed to have adju... Clinical trials have shown beneficial effects of probiotics on inflammatory bowel diseases (IBD), although the exact mechanism remains unknown. VSL#3, a mixture of 8 probiotic bacteria, has been confirmed to have adjunctive therapeutic effects on colitis. T follicular helper (Tfh) cells, a new separate subset of CD4+ T helper cells, have been proved to play a vital role in autoimmunity. The present study aimed to identify the beneficial effect of the probiotic mixture VSL#3 on the mouse model of colitis by regulating Tfh cells. Dextran sulfate sodium (DSS) was used to induce chronic colitis in C57BL/6 mice. VSL#3 (3x109 live bacteria) was given to C57BL/6 mice every other day for 60 days by gavage. The disease activity index (DAI), histological activity index (HAI), colon length and myeloperoxidase (MPO) activity were detected. Immunofluorescence was used to visualize the location of Tfh cells. Immunoglobulins, Tfh cells and plasma cells were quantified by enzyme-linked immunosorbent assay (ELISA), flow cytometry, real-time PCR or Western blotting. The results showed that after DSS treatment, the humoral immunity was disordered in C57BL/6 mice, with increased IgM, IgG and IgA levels in colonic mucus and increased Tfh cells in mesenteric lymph nodes (MLN). VSL#3 treatment showed anti-inflammatory effects as evidenced by reduced DAI score, HAI score and MPO activity. IgM, IgG and IgA levels were significantly reduced in colon mucus, and the number of Tfh cells was markedly decreased in MLN after VSL#3 treatment. It was concluded that VSL#3 alleviates DSS-induced colitis by downregulating Tfh cells, and Tfh cells may become a potential therapeutic target for IBD. 展开更多
关键词 T FOLLICULAR HELPER cells PROBIOTICS VSL#3 HUMORAL immunity inflammatory BOWEL disease
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