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Long noncoding RNA LINC00520 prevents the progression of cutaneous squamous cell carcinoma through the inactivation of the PI3K/Akt signaling pathway by downregulating EGFR
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作者 Xue-Ling Mei Shah Zhong 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第4期454-465,共12页
Background: Long noncoding RNAs (lncRNAs) play pivotal roles in various malignant tumors. Epidermal growth factor receptor (EGFR) signaling is associated with the pathogenesis of cutaneous squamous cell carcinoma (cSC... Background: Long noncoding RNAs (lncRNAs) play pivotal roles in various malignant tumors. Epidermal growth factor receptor (EGFR) signaling is associated with the pathogenesis of cutaneous squamous cell carcinoma (cSCC). This study aimed to explore the role of LINC00520 in the development of cSCC via EGFR and phosphoinositide 3-kinase-protein kinase B (PI3K/Akt) signaling pathways. Methods: A microarray analysis was applied to screen differentially expressed lncRNAs in cSCC samples. The A431 cSCC cell line was transfected and assigned different groups. The expression patterns of LINC00520, EGFR, and intermediates in the PI3K/Akt pathway were characterized using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting analysis. Cell proliferation, migration, and invasion were detected using the MTT assay, scratch test, and Transwell assay, respectively. Cell-based experiments and a tumorigenicity assay were conducted to assess the effect of LINC00520 on cSCC progression. This study was ended in September 2017. Comparisons between two groups were analyzed with t-test and comparisons among multiple groups were analyzed using one-way analysis of variance. The nonparametric Wilcoxon rank sum test was used to analyze skewed data. The enumerated data were analyzed using the chi-square test or Fisher exact test. Results: Data from chip GSE66359 revealed depletion of LINC00520 in cSCC. Cells transfected with LINC00520 vector and LINC00520 vector + si-EGFR showed elevated LINC00520 level but decreased levels of the EGFR, PI3K, AKT, VEGF, MMP-2 and MMP-9 mRNAs and proteins, and inhibition of the growth, migration and adhesion of cSCC cells, while the si-LINC00520 group showed opposite trends (all P < 0.05). Compared with the LINC00520 vector group, the LINC00520 vector + si-EGFR group showed decreased levels of the EGFR, PI3K, AKT, VEGF, MMP-2 and MMP-9 mRNAs and proteins, and inhibition of the growth, migration and adhesion of cSCC cells, while the LINC00520 vector+ EGFR vector group show 展开更多
关键词 LINC00520 EGFR PI3K/AKT signaling pathway CUTANEOUS SQUAMOUS cell carcinoma LYMPHATIC vessel INVASION INVASION Metastasis
长链非编码RNA LINC00520在喉鳞状细胞癌中的表达与临床意义 被引量:1
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作者 吴勇延 高伟 +6 位作者 张宇良 牛敏 崔佳佳 相彩霞 桑江玮 温树信 王斌全 《临床耳鼻咽喉头颈外科杂志》 北大核心 2018年第2期91-95,共5页
目的:研究长链非编码RNA LINC00520在喉鳞状细胞癌中的表达及与临床意义。方法:使用实时荧光定量PCR技术检测LINC00520在65例喉鳞状细胞癌组织及癌旁正常黏膜组织中的表达情况,分析LINC00520表达水平与患者临床分型、组织分化、淋巴... 目的:研究长链非编码RNA LINC00520在喉鳞状细胞癌中的表达及与临床意义。方法:使用实时荧光定量PCR技术检测LINC00520在65例喉鳞状细胞癌组织及癌旁正常黏膜组织中的表达情况,分析LINC00520表达水平与患者临床分型、组织分化、淋巴结转移、临床分期等临床病理参数的关系。结果:喉鳞状细胞癌LINC00520表达水平显著高于癌旁正常黏膜组织(P〈0.000 1);结合患者临床病理参数分析发现,声门型、声门上型和声门下型喉鳞状细胞癌LINC00520表达水平组间比较均差异无统计学意义(P〉0.05)。低分化组喉鳞状细胞癌中LINC00520表达水平与高、中分化组相比,差异无统计学意义(P〉0.05)。T1+T2组喉鳞状细胞癌与T3+T4组相比,LINC00520表达亦差异无统计学意义(P〉0.05)。无淋巴结转移组喉鳞状细胞癌LINC00520表达水平显著低于淋巴结转移组(P〈0.01)。结论:LINC00520表达上调可能是喉鳞状细胞癌转移中的重要分子事件并发挥重要生物学功能。 展开更多
关键词 长链非编码RNA LINC00520 喉肿瘤 肿瘤转移
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