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Assignment of unanchored scaffolds in genome of Brassica napus by RNA-seq analysis in a complete set of Brassica rapa-Brassica oleracea monosomic addition lines 预览
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作者 HUO Dong-ao ZHU Bin +3 位作者 TIAN Gui-fu DU Xu-ye GUO Juan CAI Meng-xian 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第7期1541-1546,共6页
The economically valuable oil crop Brassica napus(AACC,2 n=38),which arose from interspecific hybridization between the diploid ancestors Brassica rapa(AA,2 n=20) and Brassica oleracea(CC,2 n=18),has a complex genome.... The economically valuable oil crop Brassica napus(AACC,2 n=38),which arose from interspecific hybridization between the diploid ancestors Brassica rapa(AA,2 n=20) and Brassica oleracea(CC,2 n=18),has a complex genome.More than 10% of the assembled sequences,most of which belong to the C subgenome,have not been anchored to the corresponding chromosome.Previously,a complete set of monosomic alien addition lines(MAALs,C1–C9) with each of the nine C-subgenome chromosomes added to the extracted A subgenome was obtained from the allotetraploid B.napus donor Oro,after the ancestral B.rapa(RBR Oro) genome was restored.These MAALs effectively reduced the complexity of the B.napus genome.Here,we determined the expression values of genes on unanchored scaffolds in the MAALs and RBR Oro.Then,multiple comparisons of these gene expression values were used to determine the affiliations of the nonanchored scaffolds on which the genes were located.In total,54.68%(44.11 Mb) of the 80.67 Mb of non-anchored scaffolds belonging to the C subgenome were assigned to corresponding C chromosomes.This work highlights the potential value of these MAALs in improving the genome quality of B.napus. 展开更多
关键词 Brassica NAPUS GENOME sequence RNA-SEQ monosomic ALIEN addition lines scaffolds
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Clubroot resistance introgression in interspecific hybrids between Raphanus sativus and Brassica napus 预览
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作者 Xiuzhen Wang Lingyi Zeng +8 位作者 Li Xu Wang Chen Fan Liu Huan Yang Peng Chi Li Ren Ruibin Yan Guangyuan Lu Xiaoping Fang 《Oil Crop Science-中国油料作物学报英文版》 2019年第3期139-151,共13页
Clubroot is a prevailing soil-borne disease affecting rapeseed production worldwide.However,few clubroot resistant rapeseed accessions were available for breeding.Identification and introgression of new clubroot resis... Clubroot is a prevailing soil-borne disease affecting rapeseed production worldwide.However,few clubroot resistant rapeseed accessions were available for breeding.Identification and introgression of new clubroot resistant genes from closely related species by distant hybridization is an effective strategy.In the present study,9 radish(Raphanus sativus L.,2n=18,RR)lines resistant to Plasmodiophora brassicae pathotype 4 were used as donors to transfer clubroot resistance into a susceptible rapeseed(Brassica napus L.,2n=38,AACC)line by distant hybridization combined with embryo rescue.Nine intergeneric crosses were made but only 1(411×93039)produced F1 plants both from embryo rescue and natural seed-setting.Authenticity of triploid F1 hybrids(2n=28,ACR)were verified by flower color,cytological observation and molecular marker analysis,and 2 genuine F1 hybrids were identified.After chromosome doubling,these synthetic allohexaploid plants(2n=56,AACCRR)became partially fertile(pollen viability rate=35%)and were backcrossed with rapeseed parent to generate a BC1 population(2n=47,AACCR).Totally 178 BC1 plants were obtained,of which the majority(96.1%)were resistant to clubroot.These backcrossing progenies could be used for the breeding of new rapeseed varieties resistant to clubroot. 展开更多
关键词 CLUBROOT Plasmodiophora brassicae Brassica napus RADISH intergenerichybridization
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Effects of harvesting method and date on yield loss and seed quality of rapeseed 预览
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作者 Min Zhang Chongyou Wu +3 位作者 Mei Jin Senlin Mu Suning Liang Qing Tang 《Oil Crop Science-中国油料作物学报英文版》 2019年第3期166-174,共9页
China is one of the major rapeseed production countries in the world,but harvesting mechanization was still backward,and high harvest loss was a key inhibiting factor for rapeseed production.To obtain optimum harvesti... China is one of the major rapeseed production countries in the world,but harvesting mechanization was still backward,and high harvest loss was a key inhibiting factor for rapeseed production.To obtain optimum harvesting date for winter rape in the Yangtze River Valley of China,artificial simulated combine harvesting and artificial two-stage harvesting were adopted to find correlations between harvesting date,rape variety,seed oil content,protein content,grain moisture rate,rapeseed straw moisture content,rapeseed unthreshing rate,grain drop loss rate,thousand seeds weight(TSW),and harvesting economic coefficient(HEC).Analysis of variance showed that rapeseed oil content,protein content and TSW were correlated with rape variety;HEC was correlated with harvesting method and rape variety.Rape variety was the dominant factor of rapeseed oil content,protein content,TSW and HEC.Grain moisture rate,un-threshing rate,grain drop loss rate and straw moisture content were correlated with harvesting method and date.Harvesting date was the dominant factor of rapeseed moisture rate,un-threshing rate,grain drop loss rate and straw moisture rate.Single factor tests further proved that harvesting date had less impact on rapeseed oil content,protein content,TSW and HEC.It showed little correlation with rapeseed quality except grain moisture rate.The optimum harvesting date could be known by change in straw moisture content.The combined harvesting should be carried out during the grain moisture content of 15%-20%with TSW stabilized in the highest level.The twostage harvesting cutting should be carried out at the grain moisture content of 35%-40%.This research offered a reference to harvesting method and date for rape cultivated in the Yangtze River Valley. 展开更多
关键词 winter oilseed rape (Brassica napus L.) combine harvesting two-stageharvesting yield loss seed quality
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Soaking seed with paclobutrazol increased tolerance of juvenile oilseed rape to waterlogging 预览
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作者 Chiming Gu Yinshui Li +6 位作者 Changbing Yu Lihua Xie Xiaojia Hu Xinjie Shen Margot Brooks Xing Liao Lu Qin 《Oil Crop Science-中国油料作物学报英文版》 2019年第3期183-193,共11页
Global increase in demand for edible plant oil along with changing climates has led to a greater need to grow oilseed rape even in wet and humid environments.To find effective measures to increase tolerance of juvenil... Global increase in demand for edible plant oil along with changing climates has led to a greater need to grow oilseed rape even in wet and humid environments.To find effective measures to increase tolerance of juvenile oilseed rape to waterlogging is vitally important.A pot experiment was conducted to investigate effects of soaking seed in paclobutrazol(PBZ)solution(0(W),0.1(P1),0.3(P2)or 0.5(P3)g/L)on the growth of juvenile oilseed rape(Brassica napus L.cv.‘Zhongshuang 11’).Seed soaking with water without waterlogging was used as a control.Results showed that waterlogging decreased oilseed rape growth significantly;however,soaking seed with lower doses of PBZ(P1 and P2)significantly ameliorated the decrease in plant dry weight caused by waterlogging.Compared with W,both root dry weights and shoot dry weights of P1 and P2 treatments were significantly increased at 5 d and 10 d after waterlogging,and photosynthetic pigment contents and antioxidative enzyme activities were increased by P2 treatment at both 2 samples.Since it is labor saving and economical,soaking seed with an optimal dose(0.3 g/L)of PBZ might be an efficient way to improve waterlogging tolerance of oilseed rape. 展开更多
关键词 abiotic stress antioxidative enzymes Brassica napus CHLOROPHYLL lateral root root morphology
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Breeding of the New Hybrid Rape Variety Zhuoyou 1032 in Brassica napus L. 预览
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作者 Hailin WANG Fangming JIN +1 位作者 Yao ZHAO Hua WANG 《农业生物技术:英文版》 CAS 2019年第3期64-66,77共4页
Zhuoyou 1032 is a new double-low hybrid rape variety in Brassica napus L.bred by Guizhou Zhuohao Agricultural Technology Co.,Ltd.in 2011 with the double-low two-type 240AB in B.napus L.as the female parent and the dou... Zhuoyou 1032 is a new double-low hybrid rape variety in Brassica napus L.bred by Guizhou Zhuohao Agricultural Technology Co.,Ltd.in 2011 with the double-low two-type 240AB in B.napus L.as the female parent and the double-low restorer 132-4R as the male parent.In the adaptability evaluation test at 18 sites in two years,the average yield was 172.31 kg/666.7 m^2,which was 4.78% higher than that of Youyan 50 (CK);the whole growth period was about 202 d,which was earlier than Youyan 50 (CK) by 2-3 d;the plant height was 197.6 cm;the branching height was 52.1 cm;the number of effective branches was 5.55;the number of effective pods per plant was 179.7 (pods/plant);the number of seeds per pod was 17.7;the 1 000-seed weight was 3.99 g;and the average yield per plant was 11.7 g.It has the characteristics of early maturity,fast growth,good tidiness,compact plant type,moderate plant height,strong lodging resistance,strong resistance to sclerotinia rot,medium-to-long pods and large 1 000-seed weight. 展开更多
关键词 BRASSICA NAPUS L. Zhuoyou 1032 YIELD OIL production
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Genetic dissection of root morphological traits as related to potassium use efficiency in rapeseed under two contrasting potassium levels by hydroponics
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作者 Xiaoling Dun Jiaqin Shi +3 位作者 Huiping Liu Jie Wang Xinfa Wang Hanzhong Wang 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第6期746-757,共12页
To reveal the genetic basis of potassium use efficiency(KUE) in rapeseed, root morphology(RM), biomass and KUE-related traits were measured in a recombinant inbred line population with 175 F7 lines that were subjected... To reveal the genetic basis of potassium use efficiency(KUE) in rapeseed, root morphology(RM), biomass and KUE-related traits were measured in a recombinant inbred line population with 175 F7 lines that were subjected to high-potassium(HK) and low-potassium(LK) treatments by hydroponics. A total of 109 significant QTLs were identified to be associated with the examined traits. Sixty-one of these QTLs were integrated into nine stable QTLs. The higher heritability for RM and biomass traits and lower heritability for KUE-related traits, as well as nine stable QTLs for RM traits and only two for KUE-related traits,suggested that regulating RM traits would be more effective than selecting KUE traits directly to improve KUE by markerassisted selection. Furthermore, the integration of stable QTLs identified in the HK, LK, high-nitrogen(HN) and low-nitrogen(LN) conditions gave 10 QTL clusters. Seven of these clusters were classified into major QTLs that explained 7.4%–23.7% of the total phenotypic variation. Five of the major QTL clusters were detected under all of the treated conditions, and four clusters were specifically detected under the LK and LN conditions. These common and specific QTL clusters may be useful for the simultaneous improvement of multiple traits by marker-assisted selection. 展开更多
关键词 Brassica NAPUS L. ROOT traits potassium efficiency QTL mapping QTL cluster
luminating Crop Adaptation Using Population Genomics
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作者 Rod J.Snowdon Sarah Schiessl 《分子植物:英文版》 SCIE CAS CSCD 2019年第1期27-29,共3页
In this issue,Wu et al.(2019)describe the largest whole-genome resequencing dataset published to date for rapeseed (Brassica napus),an allopolyploid crop species that originated just a few thousand years ago under ant... In this issue,Wu et al.(2019)describe the largest whole-genome resequencing dataset published to date for rapeseed (Brassica napus),an allopolyploid crop species that originated just a few thousand years ago under anthropogenic influence and rapidly evolved into one of the world's most important oilseed crops. In almost 1000 a.ccessions spanning species-wid.e germplasm for oilseed rape,a comprehensive analysis of sequence diversity related to flowering-related traits uncovered selective sweeps associated with eco-geographic adaptation and human selection,attributable particularly to divergence among homoeologs of key flowering-time regulation and ethylene synthesis/signaling genes. 展开更多
关键词 ISSUE BRASSICA NAPUS
Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence
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作者 Dezhi Wu Zhe Liang +23 位作者 Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang 《分子植物:英文版》 SCIE CAS CSCD 2019年第1期30-43,共14页
Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underly... Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of "Darmor-bzh"and "Tapidor,"respectively.We generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of B.napus by calculating the genetic diversity and linkage disequilibrium parameters.Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding. 展开更多
关键词 Brassica napus genome RESEQUENCING selective SWEEP ECOTYPE DIVERGENCE GWAS floweringtime trait
Syntenic quantitative trait loci and genomic divergence for Sclerotin;a resistance and flowering time in Brassica napus^FA
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作者 Fengqi Zhang Junyan Huang +10 位作者 Minqiang Tang Xiaohui Cheng Yueying Liu Chaobo Tong Jingyin Yu Tehrim Sadia Caihua Dong Lingyan Liu Baojun Tang Jianguo Chen Shengyi Liu 《植物学报:英文版》 SCIE CAS CSCD 2019年第1期75-88,共14页
Oilseed rape(Brassica napus)is an allotetraploid with two subgenomes descended from a common ancestor.Accordingly,its genome contains syntenic regions with many duplicate genes,some of which may have retained their or... Oilseed rape(Brassica napus)is an allotetraploid with two subgenomes descended from a common ancestor.Accordingly,its genome contains syntenic regions with many duplicate genes,some of which may have retained their original functions,whereas others may have diverged.Here,we mapped quantitative trait loci(QTL)for stem rot resistance(SRR),a disease caused by the fungus Sclerotinia sclerotiorum,and刊owering time(FT)in a recombinant inbred line population.The population was genotyped using B.napus 60K single nucleotide polymorphìsm arrays and phenotyped in six(FT)and nine(SSR)experimental conditions or environments.(n total,we detected 30 SRR QTL and 22 FT QTL and show that some of the major QTL associated with these two traits were co-Iocalized,suggesting a genetic linkage between them.Two SRR QTL on chromosome A2 and two on chromosome C2 were shown to be syntenic,suggesting the functional conservation of these regions.We used the syntenic properties of the genomic regions to exclude genes for selection candidates responsible for QTL-associated traits.For example,152 of the 185 genes could be excluded from a syntenic A2-C2 region.These findings will help to elucidate polyploid genomics in future studies,in addition to providing useful information for B.napus breeding programs. 展开更多
关键词 NAPUS OILSEED RAPE
A Novel Chimeric Mitochondrial Gene Confers Cytoplasmic Effects on Seed Oil Content in Polyploid Rapeseed (Brassica napus)
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作者 Jun Liu Wanjun Hao +7 位作者 Jing Liu Shihang Fan Wei Zhao Linbing Deng Xinfa Wang Zhiyong Hu Wei Hua Hanzhong Wang 《分子植物:英文版》 SCIE CAS CSCD 2019年第4期582-596,共15页
Cytoplasmic effects (CEs) have been discovered to influence a diverse array of agronomic traits in crops, and understanding the underlying mechanisms can help accelerate breeding programs. Seed oil content (SOC) is of... Cytoplasmic effects (CEs) have been discovered to influence a diverse array of agronomic traits in crops, and understanding the underlying mechanisms can help accelerate breeding programs. Seed oil content (SOC) is of great agricultural, nutritional, and economic importance. However, the genetic basis of CEs on SOC (CE-SOC) remains enigmatic. In this study, we use an optimized approach to sequence the cytoplasmic (plastid and mitochondrial) genomes of allotetraploid oilseed rape (Brassica napus) cultivars, 51218 and 56366, that bear contrasting CE-SOC. By combining comparative genomics and genome-wide transcriptome analysis, we identify mitochondria-encoded orf188 as a potential CE-SOC determinant gene. Functional analyses in the model system Arabidopsis thaliana and rapeseed demonstrated that orf188 governs CE-SOC and could significantly increase SOC, strikingly, through promoting the yield of ATP. Consistent with this finding, transcriptional profiling with microarray and RNA sequencing revealed that orf188 affects transcriptional reprogramming of mitochondrial energy metabolism to facilitate ATP production. Intriguingly, orf188 is a previously uncharacterized chimeric gene, and the presence of this genetic novelty endows rapeseed with positive CE-SOC. Our results shed light on the molecular basis of CEs on a key quantitative trait in polyploid crops and enrich the theory of maternal control of oil content, providing new scientific guidance for breeding high-oil rapeseed germplasms. 展开更多
关键词 CYTOPLASMIC effects seed oil content energy metabolism crop breeding Brassica NAPUS
Tissue-specific Expression of Acetolactate Synthase (ALS),Male Sterility-inducing Effect of Tribenuron-methyl and Its Effect on ALS Activity in Brassica napus L. 预览
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作者 Sanxiong FU Xiaoying ZHOU Cunkou QI 《农业生物技术:英文版》 CAS 2019年第3期1-4,12共5页
[Objectives]This study was conducted to provide a basis for the rapid identification of the drug spraying effect in early stage and the molecular mechanism of chemical hybridizing in Brassica napus L.[Methods]Quantita... [Objectives]This study was conducted to provide a basis for the rapid identification of the drug spraying effect in early stage and the molecular mechanism of chemical hybridizing in Brassica napus L.[Methods]Quantitative RT-PCR analysis showed that ALS was constitutively expressed in various tissues of 096030,including flower buds,four floral organs (calyxes,petals,stamens and pistils),roots,stems and leaves.ALS was prominently expressed in leaves and was expressed weakly in the petals and stamens.The male sterility-inducing effects of tribenuron-methyl on such two Brassica napus L.varieties as Ningyou18 and 096030 were investigated.[Results]Plants were twice sprayed with 0.2 μg/ml tribenuron-methyl on leaves.The results showed that 8-10 ml of tribenuron- methyl was applied per plant for the first time at bolting stage with 1-2 mm flower buds on 15-20 cm inflorescence,and the second spray was performed with 8-10 ml of tribenuron-methyl per plant 10 d later.The results showed that the percentage of the full sterile plants reached 100%,which lasted for the whole flowering period,and the relative seed setting rate was only about 4%.Thus,this method could fullfill the requirement of hybrid seed production in field.The in-vivo enzyme activity of acetolactate synthase (ALS) was assayed using 2 mm buds collected 3 d after spray.The results showed that 0.2 μg/ml tribenuron-methyl inhibited ALS activity.The ALS activity of Ningyou 18 (CK) and Ningyou 18 (0.2 μg/ml) was 3.20 and 1.30 μmol/(mg·h),respectively,and the ALS activity of 096030 (CK) and 096030 (0.2 μg/ml) was 3.37 and 1.25 μmol/(mg·h),respectively.The relative enzyme activity of ALS in Ningyou18 and 096030 was 40.63% and 37.23%,respectively,both of which decreased significantly.[Conclusions]These results showed that the change of ALS activity may be used as an index for quickly identifying and predicting the chemical hybridizing effect of tribenuron-methyl. 展开更多
关键词 BRASSICA NAPUS L. TRIBENURON-METHYL Male STERILITY Expression analysis Acetolactate SYNTHASE
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Screening and Identification of Waterlogging Tolerance in Brassica napus Germplasm Resources 预览
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作者 Yun LI Cunkou QI +2 位作者 Sanxiong FU Feng CHEN Song CHEN 《农业生物技术:英文版》 CAS 2019年第1期17-20,共4页
In order to evaluate,screen and identify waterlogging-tolerant Brassica napus resources and provide good germplasm materials for breeding of waterlogging-tolerant rape,608 B.napus germplasm materials from different so... In order to evaluate,screen and identify waterlogging-tolerant Brassica napus resources and provide good germplasm materials for breeding of waterlogging-tolerant rape,608 B.napus germplasm materials from different sources were identified and screened for waterlogging-tolerant germplasms.The identification results showed that tested materials had dead seedling rates in the range of 0-100%,indicating that these rape germplasm materials varied extensively in waterlogging tolerance.Among the 608 materials,waterlogging-tolerant materials(grade I,relative dead seedling rate≤21%)accounted for 25.49%;non-tolerant materials(gradeⅣ+V,dead seedling rate≥61%)accounted for 29.43%;and other materials had waterlogging tolerance between the two.The identification results of waterlogging tolerance in the 349 rape germplasm resource from the upper,middle and lower reaches of Yangtze River and Huang-Huai Basin showed that materials from the lower Yangtze River had the lowest average dead seedling rate of 38.8%,which was remarkably lower than materials from other ecological regions,and waterlogging-tolerant materials among them accounted for 33.6%,which was also remarkably higher than materials from other ecological regions.It could thus be seen that materials from the lower Yangtze River have stronger waterlogging tolerance than those from other ecological regions overall.In this study,24 highly-tolerant B.napus germplasms(dead seedling rate<1%)were selected from the 608 B.napus germplasm materials,which could be used for breeding of waterlogging-tolerant rape and related fundamental research. 展开更多
关键词 Brassica NAPUS IDENTIFICATION of WATERLOGGING tolerance GERMPLASM SCREENING
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Transcriptome landscape of the early Brassica napus seed^FA
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作者 Dylan J.Ziegler Deirdre Khan +2 位作者 Jenna L.Kalichuk Michael G.Becker Mark F.Belmonte 《植物学报:英文版》 SCIE CAS CSCD 2019年第5期639-650,共12页
Brassica napus L.(canola) is one of the world’s most economically important oilseeds. Despite our growing knowledge of Brassica genetics, we still know little about the genes and gene regulatory networks underlying e... Brassica napus L.(canola) is one of the world’s most economically important oilseeds. Despite our growing knowledge of Brassica genetics, we still know little about the genes and gene regulatory networks underlying early seed development. In this work, we use laser microdissection coupled with RNA sequencing to profile gene activity of both the maternal and filial subregions of the globular seed. We find subregions of the chalazal end including the chalazal endosperm, chalazal proliferating tissue, and chalazal seed coat, have unique transcriptome profiles associated with hormone biosynthesis and polysaccharide metabolism. We confirm that the chalazal seed coat is uniquely enriched for sucrose biosynthesis and transport, and that the chalazal endosperm may function as an important regulator of the maternal region through brassinosteroid synthesis. The chalazal proliferating tissue,a poorly understood subregion, was specifically enriched in transcripts associated with megasporogenesis and trehalose biosynthesis, suggesting this ephemeral structure plays an important role in both sporophytic development and carbon nutrient balance, respectively. Finally, compartmentalization of transcription factors and their regulatory circuits has uncovered previously unknown roles for the chalazal pole in early seed development. 展开更多
关键词 BRASSICA NAPUS L.(canola) understood subregion
Mapping loci controlling fatty acid profiles, oil and protein content by genome-wide association study in Brassica napus 预览
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作者 Minqiang Tang Yuanyuan Zhang +6 位作者 Yueying Liu Chaobo Tong Xiaohui Cheng Wei Zhu Zaiyun Li Junyan Huang Shengyi Liu 《作物学报:英文版》 CAS CSCD 2019年第2期217-226,共10页
Optimizing the profile and quantity of fatty acids in rapeseed (Brassica napus L.) is critical for maximizing the value of edible oil and biodiesel.However,selection of these complex seed quality traits is difficult b... Optimizing the profile and quantity of fatty acids in rapeseed (Brassica napus L.) is critical for maximizing the value of edible oil and biodiesel.However,selection of these complex seed quality traits is difficult before haplotypes controlling their contents are identified.To efficiently identify genetic loci influencing these traits and underlying candidate genes and networks,we performed a genome-wide association study (GWAS) of eight seed quality traits (oil and protein content,palmitic,stearic,oleic,linoleic,eicosenoic and erucic acids content).The GWAS population comprised 370 diverse accessions,whichwere phenotyped in five environments and genotyped using 60K SNP arrays.The results indicated that oil and protein contents generally showed negative correlations,while fatty acid contents showed positive or negative correlations,with palmitic and erucic acid contents directly affecting oil content.Seven SNPs on five chromosomes were associated with both seed oil and protein content,and five genes orthologous to genes in Arabidopsis thaliana were predicted as candidates.From resequencing data,besides known haplotypes in BnaA.FAE1.a and BnaC.FAE1.a,three accessions harboring a new haplotype conferring moderate erucic acid content were identified.Interestingly,in a haplotype block,one haplotype was associated with high palmitic acid content and low oil content,while the others showed the reverse effects.This findingwas consistentwith a negative correlation between palmitic acid and oil contents,suggesting historical selection for high oil content.The identification by this study of genetic variation and complex correlations of eight seed quality traitsmay be beneficial for crop selection strategies. 展开更多
关键词 BRASSICA NAPUS OIL content Fatty acid profiles Association study Haplotypes
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Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus 预览
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作者 Jian Wu Peipei Chen +6 位作者 Qing Zhao Guangqin Cai Yue Hu Yang Xiang Qingyong Yang Youping Wang Yongming Zhou 《作物学报:英文版》 CAS CSCD 2019年第2期227-237,共11页
Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most devastating diseases of Brassica napus worldwide.Both SSR resistance and flowering time (FT) adaptation are major breedin... Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most devastating diseases of Brassica napus worldwide.Both SSR resistance and flowering time (FT) adaptation are major breeding goals in B.napus.However,early maturing rapeseed varieties,which are important for rice-rapeseed rotation in China,are often highly susceptible to SSR.Here,we found that SSR resistance was significantly negatively correlated with FT in a natural population containing 521 rapeseed inbred lines and a double haploid (DH) population with 150 individual lines,both of which had great variation in FT.Four chromosomal regions on A2,A6,C2,and C8 affecting both SSR resistance and FT were identified using quantitative trait loci (QTL) mapping after constructing a high-density genetic map based on single nucleotide polymorphism markers in the DH population.Furthermore,we aligned QTL for the two traits identified in the present and previous studies to the B.napus reference genome,and identified four colocalized QTL hotspots of SSR resistance and FT on A2 (0–7.7 Mb),A3 (0.8–7.5 Mb),C2 (0–15.2 Mb),and C6 (20.2–36.6 Mb).Our results revealed a genetic link between SSR resistance and FT in B.napus,which should facilitate the development of effective strategies in both early maturing and SSR resistance breeding and in map-based cloning of SSR resistance QTL. 展开更多
关键词 SCLEROTINIA stem ROT Brassica NAPUS QTL mapping FLOWERING time SNP array
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QTL mapping for yield components of Brassica napus L.using double haploid and immortalized F2 populations 预览
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作者 Hui Wang Wenhui Huang +6 位作者 Jun Wang Jia Liu Wenxiang Wang Li Fu QAMAR U Zaman Qiong Hu Desheng Mei 《Oil Crop Science-中国油料作物学报英文版》 2018年第4期203-214,共12页
Effective silique number per plant(ESN),seeds per silique(SS),thousand seeds weight(TSW),silique length(SL)and silique density(SD)are important seed yield potential determinant traits in rapeseed(Brassica napus L.),wh... Effective silique number per plant(ESN),seeds per silique(SS),thousand seeds weight(TSW),silique length(SL)and silique density(SD)are important seed yield potential determinant traits in rapeseed(Brassica napus L.),which are controlled by quantitative trait loci(QTL).Mapping QTL to delimited chromosomal region offers an effective method for genetic dissection of these traits.A set of 96 double haploid(DH)lines were developed by crossing 2 Brassica napus lines R1 and R2,and an immortalized F2(IF2)population containing 124 combinations was developed by crossing those DH lines.DH populations were planted at 2 locations for 2 years and IF2 populations were planted in 2 locations for 1 year.Based on the established 2,217.2 cM length high density genetic map,42 QTLs were identified,with 26 QTLs detected repeatedly in different environments or populations,including 8 for SL,7 for TSW,4 for ESN,4 for SS and 3 for SD.Among these identified QTLs,3,4,1,1 and 3 QTLs were considered as major QTLs for SL,TSW,ESN,SS and SD,respectively.In addition,2 QTLs on A9 chromosome which control multiple traits were identified.These results warrant further study of fine mapping for yield and yield components. 展开更多
关键词 Brassica NAPUS L. quantitative TRAIT locus(QTL) single NUCLEOTIDE polymorphism(SNP) yield component traits
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Genome-wide identification,phylogenetic and expression analysis of SMAX1-Like genes in rapeseed(Brassica napus L.) 预览
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作者 Hongli Yang Xiaokang Li +3 位作者 Jinglin Liu Hongfang Liu Wei Hua Ming Zheng 《Oil Crop Science-中国油料作物学报英文版》 2018年第2期71-85,共15页
SMAX1-LIKE(SMXL)family members have played important roles in regulating plant growth and development in Arabidopsis and rice,but few have been studied in Brassica napus(rapeseed).In this study,31 SMXL genes(BnaSMXL)w... SMAX1-LIKE(SMXL)family members have played important roles in regulating plant growth and development in Arabidopsis and rice,but few have been studied in Brassica napus(rapeseed).In this study,31 SMXL genes(BnaSMXL)were identified in rapeseed by whole genome bioinformatics.Detailed information were characterized,including genomic distribution,phylogenetic relationship,evolutionary selection pattern,structural and motif features.Phylogenetic analysis classified BnaSMXL genes into 4 distinct sub-clades representing clear orthologous relationships to their family members in Arabidopsis and rice.Conserved motif analysis indicated that the motif 16 mediating strigolactones(SLs)or karrikins(KARs)signaling was absent in SMXL3-5 genes.Orthologous gene pairs between Arabidopsis and 3 Brassica species were extracted,and 8 SMXL genes in Arabidopsis genome had 16,16 and 31 corresponding genes in B.oleracea,B.rapa and rapeseed genomes respectively.Evolutionary selection pattern showed that most of SMXL genes had undergone stronger negative selection.BnaSMXLs expression showed diverse patterns in 10 tissues of both vegetative and reproductive organs.Subcellular localization analysis showed that BnaSMXL proteins were exclusively localized in nucleus. 展开更多
关键词 Brassica NAPUS SMXL PHYLOGENETIC analysis expression profile SUBCELLULAR localization
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NMR-based metabolomics profile analysis in understanding metabolic variations of BraLTP1-overexpressing Brassica napus 预览
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作者 Nini Tian Pandi Wang +3 位作者 Xiaojuan Xiong Ping Fu Gang Wu Fang Liu 《Oil Crop Science-中国油料作物学报英文版》 2018年第1期1-11,共11页
BraLTP1 is an important member of lipid transfer protein family in Brassica napus.The aim of current study was to detect,classify and follow variations in metabolite profiling of B.napus with overexpression of BraLTP1... BraLTP1 is an important member of lipid transfer protein family in Brassica napus.The aim of current study was to detect,classify and follow variations in metabolite profiling of B.napus with overexpression of BraLTP1.In this study,metabolic change in leaves of BraLTP1-overexpressing B.napus plants(BraLTP1-22)and negative control(BraLTP1-22N)was investigated using nuclear magnetic resonance(NMR).Statistical strategy of principle component analysis(PCA)was performed to identify related difference metabolites.PCA score plots indicated not only high reproducibility of various treatments,but also significant difference of metabolite levels between different treatments.PCA loading plots indicated main responsible metabolites and a total of 50 metabolites were quantitatively determined.A wide range of metabolites were detected due to BraLTP1 overexpression,including biosynthesis and metabolism of sugars,amino acids,ammoniums compounds and organic acids.Furthermore,concentrations of 17 amino acids were determined for other set of samples by amino acids component analysis assay.The link between metabolite variations and phenotype were also discussed in BraLTP1-overexpressing B.napus.This work will help to gain insight into BraLTP1 function in B.napus in metabolism. 展开更多
关键词 BRASSICA NAPUS BraLTP1 lipid transfer protein NMR based metabolomics amino ACIDS component analysis
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Identification of seed heat tolerance and its correlation with agronomic traits in Brassica napus L. 预览
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作者 Guizhen Gao Kun Xu +5 位作者 Biyun Chen Xi Cheng Guixin Yan Jiangwei Qiao Qian Huang Xiaoming Wu 《Oil Crop Science-中国油料作物学报英文版》 2018年第1期21-32,共12页
To better understand the effect of high temperature on seed viability,artificial aging treatments were implemented on 472 accessions of rapeseed(Brassica napus L.)by constant high temperature at 70oC for 8h.Results sh... To better understand the effect of high temperature on seed viability,artificial aging treatments were implemented on 472 accessions of rapeseed(Brassica napus L.)by constant high temperature at 70oC for 8h.Results showed a remarkable impact of constant heat on seed germination.After heat treatment,considerable variation was found in seed germinability,some genotypes even lost the ability to germinate.The effect of accelerated aging was highly significant.Germination parameters varied among ecotypes.Seed viability varied with different origin,and was significantly and positively correlated with seed yield per plant and dry weight of aboveground parts per plant among 14 agronomic traits.Germination traits were positively correlated with oil contents. 展开更多
关键词 Brassica NAPUS L. SEED VIABILITY SEED GERMINATION heat stress AGRONOMIC traits yield quality
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高钾地力下不同镁肥用量对油菜产量和品质的影响 预览 被引量:1
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作者 李小芳 李倩 +2 位作者 雷利琴 田贵生 鲁剑巍 《湖南农业科学》 2018年第8期48-50,共3页
为了探究高钾地力下油菜(Brassica napus L.)镁肥施用效果和适宜施镁量,设4个施镁处理(0、22.5、45.0、67.5 kg/hm^2),研究不同镁肥用量对油菜产量和品质的影响。结果表明:(1)与对照(不施镁肥处理)相比,施用镁肥处理产量增加17... 为了探究高钾地力下油菜(Brassica napus L.)镁肥施用效果和适宜施镁量,设4个施镁处理(0、22.5、45.0、67.5 kg/hm^2),研究不同镁肥用量对油菜产量和品质的影响。结果表明:(1)与对照(不施镁肥处理)相比,施用镁肥处理产量增加17.1%~40.2%,当镁肥用量为45.0 kg/hm^2时产量达到最高,为2 884 kg/hm^2,当镁肥用量超过45.0 kg/hm^2后不再增加。(2)与对照(不施镁肥处理)相比,施用镁肥处理油菜籽粒含油量提高0.19~0.86个百分点,蛋白质含量降低0.08~1.77个百分点,当镁肥用量为45.0 kg/hm^2时含油量达到最高,最高含油量为45.17%,当镁肥用量超过45.0 kg/hm^2后籽粒含油量不再增加。高钾地力下,施用镁肥不仅有助于提高了油菜的产量,而且对于提高油菜籽的品质也有一定作用。湖南省衡阳地区油菜产区高钾地力下油菜生产上镁肥(硫酸镁)的适宜用量为45~60 kg/hm^2。 展开更多
关键词 高钾地力 油菜(Brassica NAPUS L.) 镁肥用量 产量 品质
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