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Skeletal muscle-derived cells repair peripheral nerve defects in mice 预览
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作者 Zi-Xiang Chen Hai-Bin Lu +3 位作者 Xiao-Lei Jin Wei-Feng Feng Xiao-Nan Yang Zuo-Liang Qi 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第1期152-161,共10页
Skeletal muscle-derived cells have strong secretory function,while skeletal muscle-derived stem cells,which are included in muscle-derived cells,can differentiate into Schwann cell-like cells and other cell types.Howe... Skeletal muscle-derived cells have strong secretory function,while skeletal muscle-derived stem cells,which are included in muscle-derived cells,can differentiate into Schwann cell-like cells and other cell types.However,the effect of muscle-derived cells on peripheral nerve defects has not been reported.In this study,5-mm-long nerve defects were created in the right sciatic nerves of mice to construct a peripheral nerve defect model.Adult female C57BL/6 mice were randomly divided into four groups.For the muscle-derived cell group,muscle-derived cells were injected into the catheter after the cut nerve ends were bridged with a polyurethane catheter.For external oblique muscle-fabricated nerve conduit and polyurethane groups,an external oblique muscle-fabricated nerve conduit or polyurethane catheter was used to bridge the cut nerve ends,respectively.For the sham group,the sciatic nerves on the right side were separated but not excised.At 8 and 12 weeks post-surgery,distributions of axons and myelin sheaths were observed,and the nerve diameter was calculated using immunofluorescence staining.The number,diameter,and thickness of myelinated nerve fibers were detected by toluidine blue staining and transmission electron microscopy.Muscle fiber area ratios were calculated by Masson’s trichrome staining of gastrocnemius muscle sections.Sciatic functional index was recorded using walking footprint analysis at 4,8,and 12 weeks after operation.The results showed that,at 8 and 12 weeks after surgery,myelin sheaths and axons of regenerating nerves were evenly distributed in the muscle-derived cell group.The number,diameter,and myelin sheath thickness of myelinated nerve fibers,as well as gastrocnemius muscle wet weight and muscle area ratio,were significantly higher in the muscle-derived cell group compared with the polyurethane group.At 4,8,and 12 weeks post-surgery,sciatic functional index was notably increased in the muscle-derived cell group compared with the polyurethane group.These criteria of the muscle-derived cel 展开更多
关键词 muscle nerve conduit MYOKINE nerve regeneration nerve repair peripheral nerve regeneration polyurethane catheter seed cells skeletal muscle skeletal muscle-derived cells tissue-engineered nerve
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Qian-Zheng-San promotes regeneration after sciatic nerve crush injury in rats 预览
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作者 Zhi-Yong Wang Li-Hua Qin +2 位作者 Wei-Guang Zhang Pei-Xun Zhang Bao-Guo Jiang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期683-691,共9页
Qian-Zheng-San,a traditional Chinese prescription consisting of Typhonii Rhizoma,Bombyx Batryticatus,Scorpio,has been found to play an active therapeutic role in central nervous system diseases.However,it is unclear w... Qian-Zheng-San,a traditional Chinese prescription consisting of Typhonii Rhizoma,Bombyx Batryticatus,Scorpio,has been found to play an active therapeutic role in central nervous system diseases.However,it is unclear whether Qian-Zheng-San has therapeutic value for peripheral nerve injury.Therefore,we used Sprague-Dawley rats to investigate this.A sciatic nerve crush injury model was induced by clamping the right sciatic nerve.Subsequently,rats in the treatment group were administered 2 mL Qian-Zheng-San(1.75 g/mL)daily as systemic therapy for 1,2,4,or 8 weeks.Rats in the control group were not administered Qian-Zheng-San.Rats in sham group did not undergo surgery and systemic therapy.Footprint analysis was used to assess nerve motor function.Electrophysiological experiments were used to detect nerve conduction function.Immunofluorescence staining was used to assess axon counts and morphological analysis.Immunohistochemical staining was used to observe myelin regeneration of the sciatic nerve and the number of motoneurons in the anterior horn of the spinal cord.At 2 and 4 weeks postoperatively,the sciatic nerve function index,nerve conduction velocity,the number of distant regenerated axons and the axon diameter of the sciatic nerve increased in the Qian-Zheng-San treatment group compared with the control group.At 2 weeks postoperatively,nerve fiber diameter,myelin thickness,and the number of motor neurons in the lumbar spinal cord anterior horn increased in the Qian-Zheng-San treatment group compared with the control group.These results indicate that Qian-Zheng-San has a positive effect on peripheral nerve regeneration. 展开更多
关键词 NERVE REGENERATION traditional Chinese medicine CRUSH INJURY peripheral NERVE REGENERATION NERVE conduction velocity SCIATIC function index NERVE INJURY NERVE repair formula SCORPION neural REGENERATION
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Repair of peripheral nerve defects by nerve transposition using small gap bio-sleeve suture with different inner diameters at both ends 预览
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作者 Yu-Hui Kou You-Lai Yu +7 位作者 Ya-Jun Zhang Na Han Xiao-Feng Yin Yu-Song Yuan Fei Yu Dian-Ying Zhang Pei-Xun Zhang Bao-Guo Jiang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期706-712,共7页
During peripheral nerve transposition repair,if the diameter difference between transposed nerves is large or multiple distal nerves must be repaired at the same time,traditional epineurial neurorrhaphy has the proble... During peripheral nerve transposition repair,if the diameter difference between transposed nerves is large or multiple distal nerves must be repaired at the same time,traditional epineurial neurorrhaphy has the problem of high tension at the suture site,which may even lead to the failure of nerve suture.We investigated whether a small gap bio-sleeve suture with different inner diameters at both ends can be used to repair a 2-mm tibial nerve defect by proximal transposition of the common peroneal nerve in rats and compared the results with the repair seen after epineurial neurorrhaphy.Three months after surgery,neurological function,nerve regeneration,and recovery of nerve innervation muscle were assessed using the tibial nerve function index,neuroelectrophysiological testing,muscle biomechanics and wet weight measurement,osmic acid staining,and hematoxylin-eosin staining.There was no obvious inflammatory reaction and neuroma formation in the tibial nerve after repair by the small gap bio-sleeve suture with different inner diameters at both ends.The conduction velocity,muscle strength,wet muscle weight,cross-sectional area of muscle fibers,and the number of new myelinated nerve fibers in the biosleeve suture group were similar to those in the epineurial neurorrhaphy group.Our findings indicate that small gap bio-sleeve suture with different inner diameters at both ends can achieve surgical suture between nerves of different diameters and promote regeneration and functional recovery of injured peripheral nerves. 展开更多
关键词 NERVE REGENERATION bio-sleeve small GAP SLEEVE SUTURE NERVE TRANSPOSITION NERVE defect NERVE conduit NERVE reinnervation peripheral NERVE neural REGENERATION
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Bridging larger gaps in peripheral nerves using neural prosthetics and physical therapeutic agents 预览
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作者 Muhammad Sana Ullah Sahar Matthew Barton Geoffrey Douglas Tansley 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1109-1115,共7页
Peripheral nerve injuries are relatively common and can be caused by a variety of traumatic events such as motor vehicle accidents.They can lead to long-term disability,pain,and financial burden,and contribute to poor... Peripheral nerve injuries are relatively common and can be caused by a variety of traumatic events such as motor vehicle accidents.They can lead to long-term disability,pain,and financial burden,and contribute to poor quality of life.In this review,we systematically analyze the contemporary literature on peripheral nerve gap management using nerve prostheses in conjunction with physical therapeutic agents.The use of nerve prostheses to assist nerve regeneration across large gaps(>30 mm)has revolutionized neural surgery.The materials used for nerve prostheses have been greatly refined,making them suitable for repairing large nerve gaps.However,research on peripheral nerve gap management using nerve prostheses reports inconsistent functional outcomes,especially when prostheses are integrated with physical therapeutic agents,and thus warrants careful investigation.This review explores the effectiveness of nerve prostheses for bridging large nerve gaps and then addresses their use in combination with physical therapeutic agents. 展开更多
关键词 NERVE repair NERVE conduits NERVE PROSTHESIS PHYSICAL therapeutic agents electric stimulation NERVE gap AXONAL growth NERVE regeneration
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Aligned fibers enhance nerve guide conduits when bridging peripheral nerve defects focused on early repair stage 预览
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作者 Qi Quan Hao-Ye Meng +7 位作者 Biao Chang Guang-Bo Liu Xiao-Qing Cheng He Tang Yu Wang Jiang Peng Qing Zhao Shi-Bi Lu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期903-912,共10页
Nerve conduits enhance nerve regeneration in the repair of long-distance peripheral nerve defects.To help optimize the effectiveness of nerve conduits for nerve repair,we developed a multi-step electrospinning process... Nerve conduits enhance nerve regeneration in the repair of long-distance peripheral nerve defects.To help optimize the effectiveness of nerve conduits for nerve repair,we developed a multi-step electrospinning process for constructing nerve guide conduits with aligned nanofibers.The alignment of the nerve guide conduits was characterized by scanning electron microscopy and fast Fourier transform.The mechanical performance of the nerve guide conduits was assessed by testing for tensile strength and compression resistance.The biological performance of the aligned fibers was examined using Schwann cells,PC12 cells and dorsal root ganglia in vitro.Immunohistochemistry was performed for the Schwann cell marker S100 and for the neurofilament protein NF200 in PC12 cells and dorsal root ganglia.In the in vivo experiment,a 1.5-cm defect model of the right sciatic nerve in adult female Sprague-Dawley rats was produced and bridged with an aligned nerve guide conduit.Hematoxylin-eosin staining and immunohistochemistry were used to observe the expression of ATF3 and cleaved caspase-3 in the regenerating matrix.The recovery of motor function was evaluated using the static sciatic nerve index.The number of myelinated fibers,axon diameter,fiber diameter,and myelin thickness in the distal nerve were observed by electron microscopy.Gastrocnemius muscle mass ratio was also determined.The analyses revealed that aligned nanofiber nerve guide conduits have good mechanical properties and can induce Schwann cells,PC12 cells and dorsal root ganglia to aggregate along the length of the nanofibers,and promote the growth of longer axons in the latter two(neuronal)cell types.The aligned fiber nerve conduits increased the expression of ATF3 and cleaved caspase-3 at the middle of the regenerative matrix and at the distal nerve segment,improved sciatic nerve function,increased muscle mass of the gastrocnemius muscle,and enhanced recovery of distal nerve ultrastructure.Collectively,the results show that highly aligned nanofibers improve the perf 展开更多
关键词 NERVE REGENERATION NERVE guide conduit electrospinning peripheral NERVE injury ALIGNED fiber SCIATIC NERVE structure mechanical function NERVE scaffold NANOFIBER neural REGENERATION
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Modified contralateral C7 nerve transfer: the possibility of permitting ulnar nerve recovery is confirmed by 10 cases of autopsy 预览
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作者 Guang-Hui Hong Jing-Bo Liu +3 位作者 Yu-Zhou Liu Kai-Ming Gao Xin Zhao Jie Lao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第8期1449-1454,共6页
Contralateral C7 nerve transfer surgery is one of the most important surgical techniques for treating total brachial plexus nerve injury. In the traditional contralateral C7 nerve transfer surgery, the whole ulnar ner... Contralateral C7 nerve transfer surgery is one of the most important surgical techniques for treating total brachial plexus nerve injury. In the traditional contralateral C7 nerve transfer surgery, the whole ulnar nerve on the paralyzed side is harvested for transfer, which completely sacrifices its potential of recovery. In the present, novel study, we report on the anatomical feasibility of a modified contralateral C7 nerve transfer surgery. Ten fresh cadavers (4 males and 6 females) provided by the Department of Anatomy, Histology, and Embryology at the Medical College of Fudan University, China were used in modified contralateral C7 nerve transfer surgery. In this surgical model, only the dorsal and superficial branches of the ulnar nerve and the medial antebrachial cutaneous nerve on the paralyzed side (left) were harvested for grafting the contralateral (right) C7 nerve and the recipient nerves. Both the median nerve and deep branch of the ulnar nerve on the paralyzed (left) side were recipient nerves. To verify the feasibility of this surgery, the distances between each pair of coaptating nerve ends were measured by a vernier caliper. The results validated that starting point of the deep branch of ulnar nerve and the starting point of the medial antebrachial cutaneous nerve at the elbow were close to each other and could be readily anastomosed. We investigated whether the fiber number of donor and recipient nerves matched one another. The axons were counted in sections of nerve segments distal and proximal to the coaptation sites after silver impregnation. Averaged axon number of the ulnar nerve at the upper arm level was approximately equal to the sum of the median nerve and proximal end of medial antebrachial cutaneous nerve (left: 0.94:1;right: 0.93:1). In conclusion, the contralateral C7 nerve could be transferred to the median nerve but also to the deep branch of the ulnar nerve via grafts of the ulnar nerve without deep branch and the medial antebrachial cutaneous nerve. The advantage over traditiona 展开更多
关键词 NERVE REGENERATION brachial plexus avulsion injury NERVE transfer CONTRALATERAL C7 NERVE MODIFIED surgery deep branch of ULNAR NERVE median NERVE medial antebrachial cutaneous NERVE hand function neural REGENERATION
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Comparison between direct repair and human acellular nerve allografting during contralateral C7 transfer to the upper trunk for restoration of shoulder abduction and elbow flexion 预览
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作者 Liang Li Wen-Ting He +3 位作者 Ben-Gang Qin Xiao-Lin Liu Jian-Tao Yang Li-Qiang Gu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2132-2140,共9页
Direct coaptation of contralateral C7 to the upper trunk could avoid the interposition of nerve grafts. We have successfully shortened the gap and graft lengths, and even achieved direct coaptation. However, direct re... Direct coaptation of contralateral C7 to the upper trunk could avoid the interposition of nerve grafts. We have successfully shortened the gap and graft lengths, and even achieved direct coaptation. However, direct repair can only be performed in some selected cases, and partial procedures still require autografts, which are the gold standard for repairing neurologic defects. As symptoms often occur after autografting, human acellular nerve allografts have been used to avoid concomitant symptoms. This study investigated the quality of shoulder abduction and elbow flexion following direct repair and acellular allografting to evaluate issues requiring attention for brachial plexus injury repair. Fifty-one brachial plexus injury patients in the surgical database were eligible for this retrospective study. Patients were divided into two groups according to different surgical methods. Direct repair was performed in 27 patients, while acellular nerve allografts were used to bridge the gap between the contralateral C7 nerve root and upper trunk in 24 patients. The length of the harvested contralateral C7 nerve root was measured intraoperatively. Deltoid and biceps muscle strength, and degrees of shoulder abduction and elbow flexion were examined according to the British Medical Research Council scoring system;meaningful recovery was defined as M3–M5. Lengths of anterior and posterior divisions of the contralateral C7 in the direct repair group were 7.64 ± 0.69 mm and 7.55 ± 0.69 mm, respectively, and in the acellular nerve allografts group were 6.46 ± 0.58 mm and 6.43 ± 0.59 mm, respectively. After a minimum of 4-year follow-up, meaningful recoveries of deltoid and biceps muscles in the direct repair group were 88.89% and 85.19%, respectively, while they were 70.83% and 66.67% in the acellular nerve allografts group. Time to C5/C6 reinnervation was shorter in the direct repair group compared with the acellular nerve allografts group. Direct repair facilitated the restoration of shoulder abduction and elbow flexion 展开更多
关键词 NERVE REGENERATION CONTRALATERAL C7 NERVE root TRANSFER NERVE graft brachial plexus avulsion injury direct REPAIR human acellular NERVE allograft shoulder function elbow function NERVE TRANSFER phrenic NERVE accessary NERVE neural REGENERATION
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Endogenous automatic nerve discharge promotes nerve repair: an optimized animal model 预览
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作者 Jing Rui Ying-Jie Zhou +3 位作者 Xin Zhao Ji-Feng Li Yu-Dong Gu Jie Lao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第2期306-312,共7页
Exogenous electrical nerve stimulation has been reported to promote nerve regeneration. Our previous study has suggested that endogenous automatic nerve discharge of the phrenic nerve and intercostal nerve has a posit... Exogenous electrical nerve stimulation has been reported to promote nerve regeneration. Our previous study has suggested that endogenous automatic nerve discharge of the phrenic nerve and intercostal nerve has a positive effect on nerve regeneration at 1 month postoperatively, but a negative effect at 2 months postoperatively, which may be caused by scar compression. In this study, we designed four different rat models to avoid the negative effect from scar compression. The control group received musculocutaneous nerve cut and repair. The other three groups were subjected to side-to-side transfer of either the phrenic (phrenic nerve group), intercostal (intercostal nerve group) or thoracodorsal nerves (thoracic dorsal nerve group), with sural nerve autograft distal to the anastomosis site. Musculocutaneous nerve regeneration was assessed by electrophysiology of the musculocutaneous nerve, muscle tension, muscle wet weight, maximum cross-sectional area of biceps, and myelinated fiber numbers of the proximal and distal ends of the anastomosis site of the musculocutaneous nerve and the middle of the nerve graft. At 1 month postoperatively, compound muscle action potential amplitude of the biceps in the phrenic nerve group and the intercostal nerve group was statistically higher than that in the control group. The myelinated nerve fiber numbers in the distal end of the musculocutaneous nerve and nerve graft anastomosis in the phrenic nerve and the intercostal nerve groups were statistically higher than those in the control and thoracic dorsal nerve groups. The neural degeneration rate in the middle of the nerve graft in the thoracic dorsal nerve group was statistically higher than that in the phrenic nerve and the intercostal nerve groups. At 2 and 3 months postoperatively, no significant difference was detected between the groups in all the assessments. These findings confirm that the phrenic nerve and intercostal nerve have a positive effect on nerve regeneration at the early stage of recovery. This study establish 展开更多
关键词 NERVE REGENERATION peripheral NERVE REGENERATION ENDOGENOUS AUTOMATIC DISCHARGE side-to-side NERVE anastomosis phrenic NERVE intercostal NERVE animal model electrical treatment rats NERVE compression neural REGENERATION
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Contralateral C7 transfer combined with acellular nerve allografts seeded with differentiated adipose stem cells for repairing upper brachial plexus injury in rats 预览
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作者 Jian-Tao Yang Jin-Tao Fang +3 位作者 Liang Li Gang Chen Ben-Gang Qin Li-Qiang Gu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1932-1940,共9页
Nerve grafting has always been necessary when the contralateral C7 nerve root is transferred to treat brachial plexus injury. Acellular nerve allograft is a promising alternative for the treatment of nerve defects, an... Nerve grafting has always been necessary when the contralateral C7 nerve root is transferred to treat brachial plexus injury. Acellular nerve allograft is a promising alternative for the treatment of nerve defects, and results were improved by grafts laden with differentiated adipose stem cells. However, use of these tissue-engineered nerve grafts has not been reported for the treatment of brachial plexus injury. The aim of the present study was to evaluate the outcome of acellular nerve allografts seeded with differentiated adipose stem cells to improve nerve regeneration in a rat model in which the contralateral C7 nerve was transferred to repair an upper brachial plexus injury. Differentiated adipose stem cells were obtained from Sprague-Dawley rats and transdifferentiated into a Schwann cell-like phenotype. Acellular nerve allografts were prepared from 15-mm bilateral sections of rat sciatic nerves. Rats were randomly divided into three groups: acellular nerve allograft, acellular nerve allograft + differentiated adipose stem cells, and autograft. The upper brachial plexus injury model was established by traction applied away from the intervertebral foramen with micro-hemostat forceps. Acellular nerve allografts with or without seeded cells were used to bridge the gap between the contralateral C7 nerve root and C5–6 nerve. Histological staining, electrophysiology, and neurological function tests were used to evaluate the effect of nerve repair 16 weeks after surgery. Results showed that the onset of discernible functional recovery occurred earlier in the autograft group first, followed by the acellular nerve allograft + differentiated adipose stem cells group, and then the acellular nerve allograft group;moreover, there was a significant difference between autograft and acellular nerve allograft groups. Compared with the acellular nerve allograft group, compound muscle action potential, motor conduction velocity, positivity for neurofilament and S100, diameter of regenerating axons, myelin sheath thickness, 展开更多
关键词 NERVE REGENERATION peripheral NERVE INJURY brachial plexus INJURY CONTRALATERAL C7 NERVE root acellular NERVE adipose stem CELLS Schwann CELLS tissue engineering NERVE NERVE grafting NERVE defect neural REGENERATION
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Axonotmesis-evoked plantar vasodilatation as a novel assessment of C-fiber afferent function after sciatic nerve injury in rats 预览
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作者 Xue-Song Wang Xue Chen +3 位作者 Tian-Wen Gu Ya-Xian Wang Da-Guo Mi Wen Hu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2164-2172,共9页
Quantitative assessment of the recovery of nerve function, especially sensory and autonomic nerve function, remains a challenge in the field of nerve regeneration research. We previously found that neural control of v... Quantitative assessment of the recovery of nerve function, especially sensory and autonomic nerve function, remains a challenge in the field of nerve regeneration research. We previously found that neural control of vasomotor activity could be potentially harnessed to evaluate nerve function. In the present study, five different models of left sciatic nerve injury in rats were established: nerve crush injury, nerve transection/ suturing, nerve defect/autografting, nerve defect/conduit repair, and nerve defect/non-regeneration. Laser Doppler perfusion imaging was used to analyze blood perfusion of the hind feet. The toe pinch test and walking track analysis were used to assess sensory and motor functions of the rat hind limb, respectively. Transmission electron microscopy was used to observe the density of unmyelinated axons in the injured sciatic nerve. Our results showed that axonotmesis-evoked vasodilatation in the foot 6 months after nerve injury/repair recovered to normal levels in the nerve crush injury group and partially in the other three repair groups;whereas the nerve defect/non-regeneration group exhibited no recovery in vasodilatation. Furthermore, the recovery index of axonotmesis-evoked vasodilatation was positively correlated with toe pinch reflex scores and the density of unmyelinated nerve fibers in the regenerated nerve. As C-fiber afferents are predominantly responsible for dilatation of the superficial vasculature in the glabrous skin in rats, the present findings indicate that axonotmesis-evoked vasodilatation can be used as a novel way to assess C-afferent function recovery after peripheral nerve injury. This study was approved by the Ethics Committee for Laboratory Animals of Nantong University of China (approval No. 20130410-006) on April 10, 2013. 展开更多
关键词 NERVE REGENERATION axonotmesis-evoked VASODILATATION laser Doppler perfusion imaging NERVE FUNCTION autonomic NERVE C-FIBER AFFERENT FUNCTION peripheral NERVE injury unmyelinated AFFERENT fiber REGENERATION neural REGENERATION
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Repair of long segmental ulnar nerve defects in rats by several different kinds of nerve transposition 预览
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作者 Fei Yu You-Lai Yu +7 位作者 Su-Ping Niu Pei-Xun Zhang Xiao-Feng Yin Na Han Ya-Jun Zhang Dian-Ying Zhang Yu-Hui Kou Bao-Guo Jiang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期692-698,共7页
Multiple regeneration of axonal buds has been shown to exist during the repair of peripheral nerve injury,which confirms a certain repair potential of the injured peripheral nerve.Therefore,a systematic nerve transpos... Multiple regeneration of axonal buds has been shown to exist during the repair of peripheral nerve injury,which confirms a certain repair potential of the injured peripheral nerve.Therefore,a systematic nerve transposition repair technique has been proposed to treat severe peripheral nerve injury.During nerve transposition repair,the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively grow into the repaired distal nerve and target muscle tissues,which is conducive to the recovery of motor function.The aim of this study was to explore regeneration and nerve functional recovery after repairing a long-segment peripheral nerve defect by transposition of different donor nerves.A long-segment(2 mm)ulnar nerve defect in Sprague-Dawley rats was repaired by transposition of the musculocutaneous nerve,medial pectoral nerve,muscular branches of the radial nerve and anterior interosseous nerve(pronator quadratus muscle branch).In situ repair of the ulnar nerve was considered as a control.Three months later,wrist flexion function,nerve regeneration and innervation muscle recovery in rats were assessed using neuroelectrophysiological testing,osmic acid staining and hematoxylin-eosin staining,respectively.Our findings indicate that repair of a long-segment ulnar nerve defect with different donor nerve transpositions can reinnervate axonal function of motor neurons in the anterior horn of spinal cord and restore the function of affected limbs to a certain extent. 展开更多
关键词 NERVE REGENERATION NERVE TRANSPOSITION REPAIR conical SLEEVE small gap SLEEVE bridging ULNAR NERVE target organ muscle NERVE reinnervation neural REGENERATION
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Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration 预览
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作者 Yao-Fa Lin Zheng Xie +2 位作者 Jun Zhou Gang Yin Hao-Dong Lin 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2183-2191,共9页
Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this i... Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this is caused by differential expression of genes and proteins during Wallerian degeneration remains unclear. The right tibial nerve and the common peroneal nerve of the same rat were exposed and completely cut through and then sutured in the same horizontal plane. On days 1, 7, 14, and 21 after surgery, 1–2 cm of nerve tissue distal to the suture site was dissected out from the tibial and common peroneal nerves. The differences in gene and protein expression during Wallerian degeneration of the injured nerves were then studied by RNA sequencing and proteomic techniques. In the tibial and common peroneal nerves, there were 1718, 1374, 1187, and 2195 differentially expressed genes, and 477, 447, 619, and 495 differentially expressed proteins on days 1, 7, 14, and 21 after surgery, respectively. Forty-seven pathways were activated during Wallerian degeneration. Three genes showing significant differential expression by RNA sequencing (Hoxd4, Lpcat4 and Tbx1) were assayed by real-time quantitative polymerase chain reaction. RNA sequencing and real-time quantitative polymerase chain reaction results were consistent. Our findings showed that expression of genes and proteins in injured tibial and the common peroneal nerves were significantly different during Wallerian degeneration at different time points. This suggests that the biological processes during Wallerian degeneration are different in different peripheral nerves after injury. The procedure was approved by the Animal Experimental Ethics Committee of the Second Military Medical University, China (approval No. CZ20160218) on February 18, 2016. 展开更多
关键词 NERVE REGENERATION PERIPHERAL NERVES PERIPHERAL NERVE injuries Wallerian degeneration TIBIAL NERVE common PERONEAL NERVE RNA sequencing proteomic real-time quantitative polymerase chain reaction neural REGENERATION
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Effect of the combination of high-frequency repetitive magnetic stimulation and neurotropin on injured sciatic nerve regeneration in rats 预览
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作者 Jie Chen Xian-Ju Zhou Rong-Bin Sun 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第1期145-151,共7页
Repetitive magnetic stimulation is effective for treating posttraumatic neuropathies following spinal or axonal injury.Neurotropin is a potential treatment for nerve injuries like demyelinating diseases.This study sou... Repetitive magnetic stimulation is effective for treating posttraumatic neuropathies following spinal or axonal injury.Neurotropin is a potential treatment for nerve injuries like demyelinating diseases.This study sought to observe the effects of high-frequency repetitive magnetic stimulation,neurotropin and their combined use in the treatment of peripheral nerve injury in 32 adult male Sprague-Dawley rats.To create a sciatic nerve injury model,a 10 mm-nerve segment of the left sciatic nerve was cut and rotated through 180°and each end restored continuously with interrupted sutures.The rats were randomly divided into four groups.The control group received only a reversed autograft in the left sciatic nerve with no treatment.In the high-frequency repetitive magnetic stimulation group,peripheral high-frequency repetitive magnetic stimulation treatment(20 Hz,20 min/d)was delivered for 10 consecutive days after auto-grafting.In the neurotropin group,neurotropin therapy(0.96 NU/kg per day)was administrated for 10 consecutive days after surgery.In the combined group,the combination of peripheral high-frequency repetitive magnetic stimulation(20 Hz,20 min/d)and neurotropin(0.96 NU/kg per day)was given for 10 consecutive days after the operation.The Basso-Beattie-Bresnahan locomotor rating scale was used to assess the behavioral recovery of the injured nerve.The sciatic functional index was used to evaluate the recovery of motor functions.Toluidine blue staining was performed to determine the number of myelinated fibers in the distal and proximal grafts.Immunohistochemistry staining was used to detect the length of axons marked by neurofilament 200.Our results reveal that the Basso-Beattie-Bresnahan locomotor rating scale scores,sciatic functional index,the number of myelinated fibers in distal and proximal grafts were higher and axon lengths were longer in the high-frequency repetitive magnetic stimulation,neurotropin and combined groups compared with the control group.These measures were not significantly different am 展开更多
关键词 AXON myelinated nerve fibers nerve regeneration neurological rehabilitation NEUROTROPIN peripheral nerve injury repetitive magnetic stimulation sciatic nerve TRAUMA
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Animal models used to study direct peripheral nerve repair:a systematic review 预览
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作者 Francisco Javier Vela Guadalupe Martínez-Chacón +3 位作者 Alberto Ballestín JoséLuis Campos Francisco Miguel Sánchez-Margallo Elena Abellán 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期491-502,共12页
Objective:Peripheral nerve repair is required after traumatic injury.This common condition represents a major public health problem worldwide.Recovery after nerve repair depends on several factors,including the severi... Objective:Peripheral nerve repair is required after traumatic injury.This common condition represents a major public health problem worldwide.Recovery after nerve repair depends on several factors,including the severity of the injury,the nerve involved,and the surgeon’s technical skills.Despite the precise microsurgical repair of nerve lesions,adequate functional recovery is not always achieved and,therefore,the regeneration process and surgical techniques are still being studied.Pre-clinical animal models are essential for this research and,for this reason,the focus of the present systematic review(according to the PRISMA statement)was to analyze the different animal models used in pre-clinical peripheral nerve repair studies.Data sources:Original articles,published in English from 2000 to 2018,were collected using the Web of Science,Scopus,and PubMed databases.Data selection:Only preclinical trials on direct nerve repair were included in this review.The articles were evaluated by the first two authors,in accordance with predefined data fields.Outcome measures:The primary outcomes included functional motor abilities,daily activity and regeneration rate.Secondary outcomes included coaptation technique and animal model.Results:This review yielded 267 articles,of which,after completion of the screening,49 studies were analyzed.There were 1425 animals in those 49 studies,being rats,mice,guinea pigs,rabbits,cats and dogs the different pre-clinical models.The nerves used were classified into three groups:head and neck(11),forelimb(8)and hindlimb(30).The techniques used to perform the coaptation were:microsuture(46),glue(12),laser(8)and mechanical(2).The follow-up examinations were histology(43),electrophysiological analysis(24)and behavioral observation(22).Conclusion:The most widely used animal model in the study of peripheral nerve repair is the rat.Other animal models are also used but the cost-benefit of the rat model provides several strengths over the others.Suture techniques are currently the first option for 展开更多
关键词 NERVE MICROSURGERY peripheral nerve regeneration repair reconstruction direct nerve repair animal model coaptation PRISMA systematic review
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Application of custom anatomy-based nerve conduits on rabbit sciatic nerve defects: in vitro and in vivo evaluations 预览
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作者 Yamuhanmode·Alike Maimaiaili·Yushan +6 位作者 Ajimu·Keremu Alimujiang·Abulaiti Zhen-Hui Liu Wei Fu Li-Wei Yan Aihemaitijiang·Yusufu Qing-Tang Zhu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2173-2182,共10页
The intermingling of regenerated nerve fibers inside nerve grafts is the main reason for mismatched nerve fibers. This is one of the key factors affecting limb function recovery after nerve injury. Previous research h... The intermingling of regenerated nerve fibers inside nerve grafts is the main reason for mismatched nerve fibers. This is one of the key factors affecting limb function recovery after nerve injury. Previous research has shown that the accuracy of axon regeneration can be improved by a bionic structural implant. To this aim, iodine and freeze-drying high-resolution micro-computed tomography was performed to visualize the 3D topography of the New Zealand rabbit sciatic nerve (25 mm). A series of 1-, 2-, 3-, and 4-custom anatomy-based nerve conduits (CANCs) were fabricated based on the anatomical structure of the nerve fascicle. The match index, luminal surface, and mechanical properties of CANCs were evaluated before implanting in a 10-mm gap of the sciatic nerve. Recovery was evaluated by histomorphometric analyses, electrophysiological study, gastrocnemius muscle weight recovery ratio, and behavioral assessments at 12 and 24 weeks postoperatively. The accuracy of nerve regeneration was determined by changes in fluorescence-labeled profile number during simultaneous retrograde tracing. Our results showed that the optimal preprocessing condition for high-resolution micro-computed tomography visualization was treatment of the sciatic nerve with 40% Lugol’s solution for 3 days followed by lyophilization for 2 days. In vitro experiments demonstrated that the match index was highest in the 3-CANC group, followed by the 2-, 1-, and 4-CANC groups. The luminal surface was lowest in the 1-CANC group. Mechanical properties (transverse compressive and bending properties) were higher in the 3- and 4-CANC groups than in the 1-CANC group. In vivo experiments demonstrated that the recovery (morphology of regenerated fibers, compound muscle action potential, gastrocnemius muscle weight recovery ratio, pain-related autotomy behaviors, and range of motion) in the 3-CANC group was superior to the other CANC groups, and achieved the same therapeutic effect as the autograft. The simultaneous retrograde tracing results showed that th 展开更多
关键词 NERVE REGENERATION NERVE conduits mismatch iodine and FREEZE-DRYING high-resolution micro-computed tomography bio-mimic CUSTOM RABBIT SCIATIC NERVE in vitro in vivo neural REGENERATION
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Different protein expression patterns in rat spinal nerves during wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling 预览
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作者 Shuai Wei Xue-Zhen Liang +12 位作者 Qian Hu Wei-Shan Wang Wen-Jing Xu Xiao-Qing Cheng Jiang Peng Quan-Yi Guo Shu-Yun Liu Wen Jiang Xiao Ding Gong-Hai Han Ping Liu Chen-Hui Shi Yu Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期315-323,共9页
Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a label... Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the differences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between s 展开更多
关键词 gene ontology Kyoto ENCYCLOPEDIA of Genes and Genomes ISOBARIC tags for RELATIVE and absolute quantitation motor NERVE PROTEOMICS sensory NERVE spinal NERVE Wallerian degeneration
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TrkA regulates the regenerative capacity of bone marrow stromal stem cells in nerve grafts 预览
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作者 Mei-Ge Zheng Wen-Yuan Sui +8 位作者 Zhen-Dan He Yan Liu Yu-Lin Huang Shu-Hua Mu Xin-Zhong Xu Ji-Sen Zhang Jun-Le Qu Jian Zhang Dong Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1765-1771,共7页
We previously demonstrated that overexpression of tropomyosin receptor kinase A(TrkA)promotes the survival and Schwann celllike differentiation of bone marrow stromal stem cells in nerve grafts,thereby enhancing the r... We previously demonstrated that overexpression of tropomyosin receptor kinase A(TrkA)promotes the survival and Schwann celllike differentiation of bone marrow stromal stem cells in nerve grafts,thereby enhancing the regeneration and functional recovery of the peripheral nerve.In the present study,we investigated the molecular mechanisms underlying the neuroprotective effects of TrkA in bone marrow stromal stem cells seeded into nerve grafts.Bone marrow stromal stem cells from Sprague-Dawley rats were infected with recombinant lentivirus vector expressing rat TrkA,TrkA-shRNA or the respective control.The cells were then seeded into allogeneic rat acellular nerve allografts for bridging a 1-cm right sciatic nerve defect.Then,8 weeks after surgery,hematoxylin and eosin staining showed that compared with the control groups,the cells and fibers in the TrkA overexpressing group were more densely and uniformly arranged,whereas they were relatively sparse and arranged in a disordered manner in the TrkA-shRNA group.Western blot assay showed that compared with the control groups,the TrkA overexpressing group had higher expression of the myelin marker,myelin basic protein and the axonal marker neurofilament 200.The TrkA overexpressing group also had higher levels of various signaling molecules,including TrkA,pTrkA(Tyr490),extracellular signal-regulated kinases 1/2(Erkl/2),pErk1/2(Thr202/Tyr204),and the anti-apoptotic proteins Bcl-2 and Bcl-xL.In contrast,these proteins were downregulated,while the pro-apoptotic factors Bax and Bad were upregulated,in the TrkA-shRNA group.The levels of the TrkA effectors Akt and pAkt(Ser473)were not different among the groups.These results suggest that TrkA enhances the survival and regenerative capacity of bone marrow stromal stem cells through upregulation of the Erk/Bcl-2 pathway.All procedures were approved by the Animal Ethical and Welfare Committee of Shenzhen University,China in December 2014(approval No.AEWC-2014-001219). 展开更多
关键词 NERVE REGENERATION bone marrow stromal stem cells TROPOMYOSIN RECEPTOR kinase A RECEPTOR LENTIVIRAL vector shRNA extracellular SIGNAL-REGULATED protein kinases 1/2 Bcl-2 NERVE grafts peripheral NERVE REGENERATION survival neural REGENERATION
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Remnant neuromuscular junctions in denervated muscles contribute to functional recovery in delayed peripheral nerve repair 预览
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作者 Leyang Li Hiroyuki Yokoyama +5 位作者 Hidetoshi Kaburagi Takashi Hirai Kunikazu Tsuji Mitsuhiro Enomoto Yoshiaki Wakabayashi Atsushi Okawa 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期731-738,共8页
Schwann cell proliferation in peripheral nerve injury(PNI)enhances axonal regeneration compared to central nerve injury.However,even in PNI,long-term nerve damage without repair induces degeneration of neuromuscular j... Schwann cell proliferation in peripheral nerve injury(PNI)enhances axonal regeneration compared to central nerve injury.However,even in PNI,long-term nerve damage without repair induces degeneration of neuromuscular junctions(NMJs),and muscle atrophy results in irreversible dysfunction.The peripheral regeneration of motor axons depends on the duration of skeletal muscle denervation.To overcome this difficulty in nerve regeneration,detailed mechanisms should be determined for not only Schwann cells but also NMJ degeneration after PNI and regeneration after nerve repair.Here,we examined motor axon denervation in the tibialis anterior muscle after peroneal nerve transection in thy1-YFP mice and regeneration with nerve reconstruction using allografts.The number of NMJs in the tibialis anterior muscle was maintained up to 4 weeks and then decreased at 6 weeks after injury.In contrast,the number of Schwann cells showed a stepwise decline and then reached a plateau at 6 weeks after injury.For regeneration,we reconstructed the degenerated nerve with an allograft at 4 and 6 weeks after injury,and evaluated functional and histological outcomes for 10 to 12 weeks after grafting.A higher number of pretzel-shaped NMJs in the tibialis anterior muscle and better functional recovery were observed in mice with a 4-week delay in surgery than in those with a 6-week delay.Nerve repair within 4 weeks after PNI is necessary for successful recovery in mice.Prevention of synaptic acetylcholine receptor degeneration may play a key role in peripheral nerve regeneration.All animal experiments were approved by the Institutional Animal Care and Use Committee of Tokyo Medical and Dental University on 5 July 2017,30 March 2018,and 15 May 2019(A2017-311C,A2018-297A,and A2019-248A),respectively. 展开更多
关键词 AXON nerve allograft nerve regeneration NEURODEGENERATION neuromuscular junction peripheral nerve injury Schwann cell skeletal muscle
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Chitosan tubes enriched with fresh skeletal muscle fibers for delayed repair of peripheral nerve defects 预览
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作者 Alessandro Crosio Benedetta Elena Fornasari +5 位作者 Giovanna Gambarotta Stefano Geuna Stefania Raimondo Bruno Battiston Pierluigi Tos Giulia Ronchi 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期1079-1084,共6页
Nerve regeneration after delayed nerve repair is often unsuccessful.Indeed,the expression of genes associated with regeneration,including neurotrophic and gliotrophic factors,is drastically reduced in the distal stump... Nerve regeneration after delayed nerve repair is often unsuccessful.Indeed,the expression of genes associated with regeneration,including neurotrophic and gliotrophic factors,is drastically reduced in the distal stump of chronically transected nerves;moreover,Schwann cells undergo atrophy,losing their ability to sustain regeneration.In the present study,to provide a three-dimensional environment and trophic factors supporting Schwann cell activity and axon re-growth,we combined the use of an effective conduit(a chitosan tube)with a promising intraluminal structure(fresh longitudinal skeletal muscle fibers).This enriched conduit was used to repair a 10-mm rat median nerve gap after 3-month delay and functional and morphometrical analyses were performed 4 months after nerve reconstruction.Our data show that the enriched chitosan conduit is as effective as the hollow chitosan conduit in promoting nerve regeneration,and its efficacy is not statistically different from the autograft,considered the“gold standard”technique for nerve reconstruction.Since hollow tubes not always lead to good results after long defects(>20 mm),we believe that the conduit enriched with fresh muscle fibers could be a promising strategy to repair longer gaps,as muscle fibers create a favorable three-dimensional environment and release trophic factors.All procedures were approved by the Bioethical Committee of the University of Torino and by the Italian Ministry of Health(approval number:864/2016/PR)on September 14,2016. 展开更多
关键词 tubulization Schwann cells SCAFFOLD tissue engineering NERVE reconstruction morphometrical analyses GRASPING test secondary repair MEDIAN NERVE NERVE REGENERATION
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Novel miRNA,miR-sc14,promotes Schwann cell proliferation and migration 预览
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作者 Xi-Meng Ji Shan-Shan Wang +5 位作者 Xiao-Dong Cai Xing-Hui Wang Qian-Yan Liu Pan Wang Zhang-Chun Cheng Tian-Mei Qian 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1651-1656,共6页
MicroRNAs refer to a class of endogenous,short non-coding RNAs that mediate numerous biological functions.MicroRNAs regulate various physiological and pathological activities of peripheral nerves,including peripheral ... MicroRNAs refer to a class of endogenous,short non-coding RNAs that mediate numerous biological functions.MicroRNAs regulate various physiological and pathological activities of peripheral nerves,including peripheral nerve repair and regeneration.Previously,using a rat sciatic nerve injury model,we identified many functionally annotated novel microRNAs,including miR-sc14.Here,we used real-time reverse transcription-polymerase chain reaction to examine miR-sc14 expression in rat sciatic nerve stumps.Our results show that miRsc14 is noticeably altered following sciatic nerve injury,being up-regulated at 1 day and diminished at 7 days.EdU and transwell chamber assay results showed that miR-sc14 mimic promoted proliferation and migration of Schwann cells,while miR-sc14 inhiThe study was approved by the Jiangsu Provincial Laboratory Animal Management Committee,China on March 4,2015(approval No.20150304-004).bitor suppressed their proliferation and migration.Additionally,bioinformatic analysis examined potential target genes of miR-sc14,and found that fibroblast growth factor receptor 2 might be a potential target gene.Specifically,our results show changes of miR-sc14 expression in the sciatic nerve of rats at different time points after nerve injury.Appropriately,up-regulation of miR-sc14 promoted proliferation and migration of Schwann cells.Consequently,miR-sc14 may be an intervention target to promote repair of peripheral nerve injury.The study was approved by the Jiangsu Provincial Laboratory Animal Management Committee,China on March 4,2015(approval No.20150304-004). 展开更多
关键词 NERVE REGENERATION novel microRNAs miR-sc14 PERIPHERAL NERVE injury cell PROLIFERATION cell MIGRATION Schwann cells fibroblast growth factor receptor 2 biological functions PERIPHERAL NERVE REGENERATION regulatory mechanisms neural REGENERATION
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