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Differential Responses of Phytophthora sojae to Seed Exudates of Host Soybean and Non-host Maize 预览
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作者 Wen Jing-zhi Zhang Zhuo-qun +6 位作者 Xu Ying Song Guang-mei Chen Yu-fei Gao Xin-ying Zhao Yu-qi Jia Meng- zhen Liang Jia-yu 《东北农业大学学报:英文版》 CAS 2019年第3期1-8,共8页
In order to clarify the differential response of Phytophthora sojae to the seed exudates of host soybean and non-host maize and understand the relationship between seed exudates and host selectivity of Phytophthora so... In order to clarify the differential response of Phytophthora sojae to the seed exudates of host soybean and non-host maize and understand the relationship between seed exudates and host selectivity of Phytophthora sojae, non-host maize Suiyu 23 and susceptible host soybean Sloan seed exudates were collected to measure their influence on mycelial growth, formation and germination of oospores, chemotaxis, encystment and germination of zoospores of Phytophthora sojae. The results showed that nonhost maize seed exudates exhibited repellency to zoospores of Phytophthora sojae, it also could significantly inhibited Phytophthora sojae mycelial growth, formation of oospores compared with the control;compared with host soybean, non-host maize seed exudates could significantly inhibited Phytophthora sojae mycelial growth, formation and germination of oospores, germination of cysts, which indicated that the seed exudates was the critical factor to host selectivity of Phytophthora sojae and the maize seed exudates was closely related to its non-host resistance. 展开更多
关键词 PHYTOPHTHORA sojae SEED EXUDATE non-host resistance
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三种单子叶植物叶组织中GWSF诱导转录特性
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作者 董永清 陈广欣 +2 位作者 伍思韵 邝嘉怡 黄真池 《分子植物育种》 CAS CSCD 北大核心 2019年第13期4257-4262,共6页
理想病原诱导型启动子的应用在植物抗病基因工程中非常重要。本研究以高粱、玉米和小麦为材料,检测人工病原诱导型启动子GWSF在单子叶植物叶组织中的诱导转录特性,寻找理想病原诱导型启动子。用GWSF替代pBI121中调控gus基因的CaMV35S启... 理想病原诱导型启动子的应用在植物抗病基因工程中非常重要。本研究以高粱、玉米和小麦为材料,检测人工病原诱导型启动子GWSF在单子叶植物叶组织中的诱导转录特性,寻找理想病原诱导型启动子。用GWSF替代pBI121中调控gus基因的CaMV35S启动子构建重组质粒,导入农杆菌(Agrobacterium tumefaciens) GV3101;通过农杆菌渗入法转化高粱、玉米和小麦12 h后,用水杨酸,青枯菌,疫霉菌孢子诱导处理12 h,本底表达用无菌水处理12 h;最后用GUS染色法和实时荧光定量PCR (real-time quantitative PCR, qPCR)检测GWSF的转录特性。GUS染色结果显示:GWSF在三种植物中都具有本底低、受水杨酸,青枯菌,疫霉菌孢子诱导的特性。设CaMV35S的转录活性为1,qPCR结果为:GWSF在高粱中本底水平为0.67,受水杨酸,青枯菌,疫霉菌孢子诱导时,转录活性明显升高,分别为:2.53、0.87、7.33;玉米中本底水平为0.11,受水杨酸,青枯菌,疫霉菌孢子诱导时,转录活性分别为:1.92、0.19、2.06;小麦中本底水平为0.13,受水杨酸,青枯菌,疫霉菌孢子诱导时,转录活性分别为:0.69、0.45、1.16。结果表明,GWSF在三种单子叶植物叶组织中具有本底低、诱导因子广、诱导活性高的特性,是较理想的人工病原诱导型启动子,可应用于单子叶植物转基因抗病育种。 展开更多
关键词 单子叶植物 GWSF 水杨酸 青枯菌 疫霉菌
Phytophthora sojae Effector PsAvh240 Inhibits Host Aspartic Protease Secretion to Promote Infection
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作者 Baodian Guo Haonan Wang +13 位作者 Bo Yang Wenjing Jiang Maofeng Jing Haiyang Li Yeqiang Xia Yuanpeng Xu Qinli Hu Fangfang Wang Feng Yu Yan Wang Wenwu Ye Suomeng Dong Weiman Xing Yuanchao Wang 《分子植物:英文版》 SCIE CAS CSCD 2019年第4期552-564,共13页
Plants secrete defense molecules into the extracellular space (the apoplast) to combat attacking microbes. However, the mechanisms by which successful pathogens subvert plant apoplastic immunity remain poorly understo... Plants secrete defense molecules into the extracellular space (the apoplast) to combat attacking microbes. However, the mechanisms by which successful pathogens subvert plant apoplastic immunity remain poorly understood. In this study, we show that PsAvh240, a membrane-localized effector of the soybean pathogen Phytophthora sojae, promotes P. sojae infection in soybean hairy roots. We found that PsAvh240 interacts with the soybean-resistant aspartic protease GmAP1 in planta and suppresses the secretion of GmAP1 into the apoplast. By solving its crystal structure we revealed that PsAvh240 contain six a helices and two WY motifs. The first two a helices of PsAvh240 are responsible for its plasma membrane-localization and are required for PsAvh240's interaction with GmAP1. The second WY motifs of two PsAvh240 molecules form a handshake arrangement resulting in a handshake-like dimer. This dimerization is required for the effector's repression of GmAP1 secretion. Taken together, these data reveal that PsAvh240 localizes at the plasma membrane to interfere with GmAP1 secretion, which represents an effective mechanism by which effector proteins suppress plant apoplastic immunity. 展开更多
关键词 PHYTOPHTHORA sojae effector handshake?like dimer soybean aspartic PROTEASE plant apoplastic immunity EFFECTOR triggered susceptibility
A Phytophthora capsid Effector Targets ACD11 Binding Partners that Regulate ROS-Mediated Defense Response in Arabidopsis
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作者 Qi Li Gan Ai +8 位作者 Danyu Shen Fen Zou Ji Wang Tian Bai Yanyu Chen Shutian Li Meixiang Zhang Maofeng Jing Daolong Dou 《分子植物:英文版》 SCIE CAS CSCD 2019年第4期565-581,共17页
Reactive oxygen species (ROS) play a vital role in plant immune response, but the genes involved in the regulation of ROS are scantily reported. Phytophthora pathogens produce a large number of effectors to promote in... Reactive oxygen species (ROS) play a vital role in plant immune response, but the genes involved in the regulation of ROS are scantily reported. Phytophthora pathogens produce a large number of effectors to promote infection, but the modes of action adopted are largely unknown. Here, we report that RxLR207 could activate ROS-mediated cell death in Nicotiana benthamiana and was essential for virulence of P. capsici. We found that this effector targeted BPA1 (binding partner of ACD11) and four members of BPLs (BPAl-Like proteins)in Arabidopsis, and the bpa1 and bpl mutants had enhanced ROS accumulation and cell death under biotic or abiotic stresses. Furthermore, we showed that BPA1 and several BPLs functioned redundantly in plant immunity to P. capsici. We discovered that BPA1 and all six BPLs interacted with ACD11, and stabilization of ACD11 was impaired in the bpa 1, bpl2, bpl3, and bpl4 mutants. RxLR207 could promote the degradation of BPA1, BPL1, BPL2, and BPL4 to disrupt ACD11 stabilization in a 26S proteasome-dependent manner. Taken together, these fin dings indicate the important roles of Arabidopsis BPA1 and its homologs in ROS homeostasis and defense response, highlighting the usefulness of a pathogen effector-directed approach as a promising strategy for the discovery of novel plant immune regulators. 展开更多
关键词 PHYTOPHTHORA ARABIDOPSIS ROS RxLR EFFECTOR ACD11 BPA1
Synthesis and Bioactivity Evaluation of Novel N-Pyridylpyrazolemethanamine Derivatives
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作者 HUA Xuewen WEI Wei +1 位作者 ZHU Liangliang ZHOU Yunyun 《高等学校化学研究:英文版》 SCIE CAS CSCD 2018年第5期744-750,共7页
关键词 衍生物 PHYTOPHTHORA 评估 合成 化学结构 混合物 生物活动 鉴定结果
诱饵模式——病原菌致病的全新机制 预览
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作者 王源超 《南京农业大学学报》 CSCD 北大核心 2018年第1期1-2,共2页
XEG1属于GH12家族糖基水解酶,能降解植物细胞壁,是疫病菌攻击植物的关键"武器"。植物在利用抑制蛋白GIP1对XEG1的防卫过程中,疫霉菌能分泌失去酶活性的XEG1突变体——XLP1作为"诱饵"吸引抑制蛋白,掩护XEG1对植物的攻击。
关键词 植物免疫 疫霉菌 致病机制 效应子 诱饵模式
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基于文献计量分析的疫霉菌抗甲霜灵研究进展 预览
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作者 刘冬 李坤缘 +2 位作者 王伟燕 李萍 高智谋 《信阳农林学院学报》 2018年第2期103-105,109共4页
疫霉菌引起的病害是植物生产上的严重威胁,甲霜灵在这些病害防治中起了关键性作用。本文综述了国内外疫霉菌对甲霜灵内吸性杀菌剂的抗药性发生现状、分子机制、环境适合度和抗药性治理对策等方面的研究进展,对疫霉菌的防治具有一定的参... 疫霉菌引起的病害是植物生产上的严重威胁,甲霜灵在这些病害防治中起了关键性作用。本文综述了国内外疫霉菌对甲霜灵内吸性杀菌剂的抗药性发生现状、分子机制、环境适合度和抗药性治理对策等方面的研究进展,对疫霉菌的防治具有一定的参考价值。 展开更多
关键词 疫霉菌 甲霜灵 抗药性 CNKI Web of SCIENCE
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木醋液对土壤镰孢菌和疫霉菌的影响-土壤培养试验 预览
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作者 张文丽 范丽 +2 位作者 林启美 赵小蓉 李贵桐 《华北农学报》 CSCD 北大核心 2018年第A01期268-272,共5页
为了研究木醋液对土壤微生物,尤其是镰孢菌及疫霉菌数量的影响,采用室内培养方法,向大棚土壤加入系列量木醋液,25℃下培养35d。结果显示,在培养期间,各处理土壤pH值变化很小。除培养第7天外,木醋液对土壤pH值没有显著的影响,只有加入量... 为了研究木醋液对土壤微生物,尤其是镰孢菌及疫霉菌数量的影响,采用室内培养方法,向大棚土壤加入系列量木醋液,25℃下培养35d。结果显示,在培养期间,各处理土壤pH值变化很小。除培养第7天外,木醋液对土壤pH值没有显著的影响,只有加入量达到3.3mL/kg,土壤pH值才显著降低了近0.2个单位。培养初期加入木醋液的土壤矿质氮含量略有降低,降低量为15.1~28.2mg/kg。但随着培养时间的延长,所有土壤矿质氮含量逐渐增加至相近的水平(324.9~344.8mg/kg)。在35d培养期间,加入不同体积木醋液的土壤,可培养细菌、真菌及放线菌数量与对照土壤仅在某些时间点有明显的差异,但在第14天时,1.3,3.3mL/kg处理的土壤可培养放线菌数量分别比比对照土壤低31.4%,34.4%;在第21天时,0.7,1.3,3.3mL/kg处理可培养细菌数量与对照相比显著降低了20.2%,24.8%,40.6%,0.3,0.7,3.3mL/kg处理可培养真菌数量与对照相比显著降低了45.9%,35.5%,36.6%。加入系列量木醋液的土壤,在培养过程中其镰孢菌和疫霉菌数量的变化很大,但与对照土壤没有显著性差异。总体来看,木醋液作为酸性液体,对土壤酸度产生一定的影响,但对土壤矿质氮、细菌、真菌、放线菌,以及镰孢菌和疫霉菌数量没有显著的影响。说明木醋液几乎没有杀灭镰孢菌和疫霉菌的作用,不适合用作土壤熏蒸剂,减轻土传病害的作用可能也很有限。 展开更多
关键词 木醋液 土壤 镰孢菌 疫霉菌
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浅谈马铃薯晚疫病的发生与防治 预览
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作者 毛元江 《新农村(黑龙江)》 2018年第17期30-30,32共2页
马铃薯晚疫病是危害我镇马铃薯的主要病害。本文根据我们近年来对马玲薯晚疫病发生、危害情况的调查及防治经验进行了认真分析总结,并根据马玲薯晚疫病的症状、病源、浸染循环及发生和流行条件等因素,结合我镇马玲薯晚疫病发生、危害情... 马铃薯晚疫病是危害我镇马铃薯的主要病害。本文根据我们近年来对马玲薯晚疫病发生、危害情况的调查及防治经验进行了认真分析总结,并根据马玲薯晚疫病的症状、病源、浸染循环及发生和流行条件等因素,结合我镇马玲薯晚疫病发生、危害情况及防治经验,提出了马铃薯晚疫病的防治措施。马铃薯晚疫病是一种真菌性病害,是我镇马铃薯的主要病害之一。在我镇马铃薯种植区域均有不同程度的发生。目前我镇种植马铃薯达6万亩,占粮食种植面积的50%以上。其中,春马铃薯达5万亩,冬早马铃薯1万亩。马铃薯即是我镇主要的粮食作物,也是主要的经济作物。由于农户种植水平较低,管理粗放,加之品种不断退化及品种更新较慢。因此,马铃薯晚疫病的发生面积及发生程度呈逐年上升趋势,该病危害较大,损失严重,给我镇马铃薯生产带来严重的损失。为此,搞好马铃薯晚疫病的防治,是保证马铃薯产量,确保农民的增产增收的重要措施。1 病原菌马铃薯晚疫病菌【Phytophthora infestans(Mont.)de Bary】属真菌中的卵菌。1.1 病菌形态病菌菌丝多核、无色、无隔。在病叶上产生的白色霉状物是病原菌的孢子梗和孢子囊。孢子梗从寄主的气孔中伸出,无色,有1-4个分枝,在每个分枝的顶端产生孢子囊。孢子囊无色、单包、卵圆形,顶端有乳突。孢子囊盟发方式分为直接盟发和间接盟发两种。间接明发是在低温高湿条件的盟发,盟发产生的芽管再次侵入寄主;直接盟发是在温度高于15℃时,孢子囊直接明发产生芽管而侵入寄主。孢子梗顶端形成孢子囊后,孢子梗可继续生长而将孢子囊推向一旁,并在顶端又再形成孢子囊,孢子囊借风雨传播,再次侵染寄主,在生产季节中病菌可多代重复侵染。1.2 寄生性及寄主晚疫病菌是一种寄生性很强的真菌,只能在特定的寄主上寄生形成孢子囊� 展开更多
关键词 马铃薯晚疫病菌 发生与防治 PHYTOPHTHORA 番茄晚疫病菌 真菌性病害 危害情况 种植区域 品种更新
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Sensitivity assessment of Phytophthora nicotianae to fungicides of fluazinam and its mixture with carboxylic acid amide 预览
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作者 MU Wenjun LI Qi +8 位作者 CHEN Xiuhua LI Haijiang DONG Kunle WANG Jianwei ZHANG Youwu FENG Xiaohu ZHANG Zhigao WANG Libing SONG Jizhen 《烟草科技》 CSCD 北大核心 2018年第A01期1-7,共7页
Tobacco black shank caused by Phytophthora parasitica var.nicotianae(Breda de Hean)is one of the most serious diseases in tobacco growing,and few effective fungicides are available currently.The purpose of this study ... Tobacco black shank caused by Phytophthora parasitica var.nicotianae(Breda de Hean)is one of the most serious diseases in tobacco growing,and few effective fungicides are available currently.The purpose of this study was to assess the feasibility of using fluazinam to treat tobacco black shank disease.In vitro toxicities of fluazinam to 62 isolates of P.nicotianae were determined by mycelial growth rate method.The EC50 values ranged from 0.294 6 to 2.389 9 μg/mL with a mean of 1.084 0 μg/mL.The sensitivity distribution was unimodal,and there was no resistant isolate in P.nicotianae population.The carboxylic acid amide(CAA)fungicides were single-site in action and activity to oomycetes.The synergistic interactions were tested by Wadley method between fluazinam and dimethomorph,flumorph,mandipropamid,respectively.The mixture containing dimethomoph:Huazinam at 1:2 ratio showed additive actions,with the synergy ratio of 1.2.The mixture containing flumorph and fluazinam at 1:5 ratio had the highest synergy ratio(SR 2.07).Mandipropamid and fluazinam had an additive interaction and the proportion of 2:1 had the lowest EC50 value(0.020 7 μg/mL).This study demonstrated the sensitivity of P.nicotianae to fluazinam,and provided the experimental foundation to use fluazinam for tobacco black shank management. 展开更多
关键词 Phytophthora nicotianae FLUAZINAM DIMETHOMORPH FLUMORPH MANDIPROPAMID FUNGICIDE MIXTURE
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Rediscovery and analysis of Phytophthora carbohydrate esterase(CE) genes revealing their evolutionary diversity 预览
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作者 QIAN Kun LI Deng-hui +5 位作者 LIN Run-mao SHI Qian-qian MAO Zhen-chuan YANG Yu-hong FENG Dong-xin XIE Bing-yan 《农业科学学报:英文版》 SCIE CAS CSCD 2018年第4期878-891,共14页
A continuous co-evolutionary arms-race between pathogens and their host plants promotes the development of pathogenicfactors by microbes, including carbohydrate esterase (CE) genes to overcome the barriers in plant ... A continuous co-evolutionary arms-race between pathogens and their host plants promotes the development of pathogenicfactors by microbes, including carbohydrate esterase (CE) genes to overcome the barriers in plant cell walls. Identificationof CEs is essential to facilitate their functional and evolutionary investigations; however, current methods may have a limitin detecting some conserved domains, and ignore evolutionary relationships of CEs, as well as do not distinguish CEs fromproteases. Here, candidate CEs were annotated using conserved functional domains, and orthologous gene detection andphylogenetic relationships were used to identify new CEs in 16 oomycete genomes, excluding genes with protease domains.In our method, 41 new putative CEs were discovered comparing to current methods, including three CE4, 14 CE5, eight CE12,five CE13, and 11 CE14. We found that significantly more CEs were identified in Phytophthora than in Hyaloperonosporaand Pythium, especially CE8, CE12, and CE13 that are putatively involved in pectin degradation. The abundance of theseCEs in Phytophthora may be due to a high frequency of multiple-copy genes, supporting by the phylogenetic distributionof CE13 genes, which showed five units of Phytophthora CE13 gene clusters each displaying a species tree like topology,but without any gene from Hyaloperonospora or Pythium species. Additionally, diverse proteins associated with productsof CE13 genes were identified in Phytophthora strains. Our analyses provide a highly effective method for CE discovery,complementing current methods, and have the potential to advance our understanding of function and evolution of CEs. 展开更多
关键词 PHYTOPHTHORA CARBOHYDRATE ESTERASE gene prediction comparative genomic ANALYSIS evolution DIVERSITY
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Development and testing of a weather-based model to determine potential yield losses caused by potato late blight and optimize fungicide application
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作者 Alexey FILIPPOV Maria KUZNETSOVA +3 位作者 AlexANDer ROGOZHIN Olga IAKUSHEVA Valentina DEMIDOVA Natalia STATSYUK 《农业科学与工程前沿:英文版》 2018年第4期462-468,共7页
Late blight is one of the most important potato diseases. To minimize yield losses, various protective measures are used including fungicide application. Active use of fungicides results in a contamination of the envi... Late blight is one of the most important potato diseases. To minimize yield losses, various protective measures are used including fungicide application. Active use of fungicides results in a contamination of the environment. Therefore, crop protection strategies optimizing the number of treatments are of great interest.Using information about late blight development in an experimental potato field recorded over 30 seasons, a simulator to forecast yield losses caused by the disease was developed based on the number of 5-d periods favorable for reinfection of plants during a vegetation season. The simulator was successfully verified using independent data on the disease development from nine unprotected potato fields in the Netherlands and Germany. The average difference between the calculated and real yield losses did not exceed 5%. Using the simulator and weather data for a period of 2007–2017, yield losses were calculated for several areas of the Bryansk, Tambov, and Orenburg Regions of Russia. The results revealed differences in disease development between these regions and may be used to develop recommendations for a frequency of fungicide applications according to the regional risk of epidemics, leading to a significant reduction in fungicide use. 展开更多
关键词 POTATO late blight PHYTOPHTHORA INFESTANS yield losses RETROSPECTIVE analysis mathematical MODEL
土传病害的危害及防治技术 预览 被引量:1
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作者 曹坳程 《营销界:农资与市场》 2017年第3期35-36,共2页
近年来,作物病虫害发生面积不断扩大,发生程度不断加重,成为制约作物产量和品质的重要因素,土传病害被人们称为作物癌症。土传病害一般危害植物根茎,作物生长前期一旦发生病害,幼苗根腐烂或茎腐烂猝倒,幼苗很快就会死亡,严重影响作物生... 近年来,作物病虫害发生面积不断扩大,发生程度不断加重,成为制约作物产量和品质的重要因素,土传病害被人们称为作物癌症。土传病害一般危害植物根茎,作物生长前期一旦发生病害,幼苗根腐烂或茎腐烂猝倒,幼苗很快就会死亡,严重影响作物生产。作物生长后期发生病害,一般年份减产20~30%,严重年份减产50~60%,甚至绝收。土传病害发病后,比较难以防治,病菌藏在土壤中越冬,很难被杀死,来年继续侵害作物,如此循环,病害越来越严重。 展开更多
关键词 土传病害 生长前期 幼苗根 植物根茎 根结线虫 马铃薯黑胫病 青枯假单胞菌 PHYTOPHTHORA 致病疫霉 土壤改良
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Status of Phytophthora and Huanglongbing Diseases of Citrus Causing Decline in Coorg Mandarin in India 预览
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作者 Priti Sonavane Venkataravanappa Venkataravanappa 《农业科学与技术:B》 2017年第4期248-263,共16页
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Synthesis and Fungicidal Activity of (E)-5-[1-(2-Oxo- 1 -oxaspiro[4,5]dec/non-3-en-3-yl)ethylidene]-2- aminoimidazolin-4-one Derivatives
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作者 Bo Tang Aiying Guan +3 位作者 Yu Zhao Jiazhen Jiang Mingan Wang Ligang Zhou 《中国化学:英文版》 SCIE CAS CSCD 2017年第7期1133-1140,共8页
关键词 杀菌活性 衍生物 DEC PHYTOPHTHORA 合成 乙基 氧代 扫描电子显微镜
草莓疫霉病病原菌鉴定 预览
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作者 汪泉 史芳芳 《中国蔬菜》 北大核心 2016年第11期46-50,共5页
采集新疆生产建设兵团第十二师农业科学研究所草莓育苗网室内典型疫霉病症状的发病草莓短缩茎,进行组织培养、病原菌分离及纯化,并进行病原菌形态学及分子生物学鉴定。结果表明:发病草莓短缩茎分离得到的培养物在PDA培养基上的形态正... 采集新疆生产建设兵团第十二师农业科学研究所草莓育苗网室内典型疫霉病症状的发病草莓短缩茎,进行组织培养、病原菌分离及纯化,并进行病原菌形态学及分子生物学鉴定。结果表明:发病草莓短缩茎分离得到的培养物在PDA培养基上的形态正反面无差异,分离纯化获得P1菌株;致病性结果表明,P1菌株在接种病原物的健康草莓短缩茎部位产生了病原菌菌丝;利用通用引物ITS1和ITS4对P1菌株DNA进行PCR扩增,得到大小约800bp的条带,获得片段与Phytophthora cactorumstrainGL1、BT1、TARI20179菌株的ITS序列同源性均为100%;结合形态学和分子生物学鉴定结果,确定致病病原菌为恶疫霉菌(Phytophthora cactorum)。 展开更多
关键词 草莓 疫霉病 恶疫霉菌 病原菌鉴定
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天然来源枯草芽孢杆菌的抑菌活性研究
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作者 吴卫刚 吴祺豪 +3 位作者 沈青青 陈姜 陈苏 王鸿 《中华中医药学刊》 CAS 北大核心 2016年第3期740-743,共4页
目的:研究一种天然来源抑菌剂对常见致病性细菌(金黄色葡萄球菌,蜡状芽孢杆菌,铜绿假单胞菌,大肠杆菌)以及石斛病原真菌(烟草疫霉菌)的抑制作用。方法:从东海沙滩泥土中分离纯化菌株,通过牛津杯法及对峙法筛选对四株致病性细菌和... 目的:研究一种天然来源抑菌剂对常见致病性细菌(金黄色葡萄球菌,蜡状芽孢杆菌,铜绿假单胞菌,大肠杆菌)以及石斛病原真菌(烟草疫霉菌)的抑制作用。方法:从东海沙滩泥土中分离纯化菌株,通过牛津杯法及对峙法筛选对四株致病性细菌和一株石斛病原真菌具有抑制作用的活性菌株;通过观察菌体形态,16S rRNA测序、Gen Bank比对,对最优活性菌株进行鉴定;通过优化菌株培养条件,获得活性物质产生最佳发酵条件。结果:从样品中共分离52株菌,6株菌发酵液提取物对病原细菌具有抑制作用,2株对烟草疫霉菌具有拮抗活性。选取活性最佳的菌株,通过鉴定,确定为枯草芽孢杆菌(Bacillus subtilis);其发酵液乙酸乙酯提取物对所述5株指示菌具有生长抑制活性;通过优化,确定该菌最佳培养条件为37℃,ISP2培养基,发酵培养96 h。结论:从东海中所分离获得的枯草芽孢杆菌对常见致病细菌具有较强的抑制作用,且对烟草疫霉菌具有抑制作用,可用于筛选石斛病原菌天然抑制剂的研究。 展开更多
关键词 枯草芽孢杆菌 抑菌活性 疫霉菌 海洋微生物
呼伦贝尔地区马铃薯主栽品种对晚疫病的抗性调查 预览
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作者 何忠仁 张玉敏 《现代农业》 2016年第3期55-56,共2页
致病疫霉(Phytophthora infestans)引起的马铃薯晚疫病是马铃薯的一种毁灭性病害,全世界普遍发生,危害严重,己列为世界粮食作物第一大病害。1950年中国许多地区晚疫病暴发,华北马铃薯当年减产30%-50%。上世纪60-70年代,由于引进抗病品... 致病疫霉(Phytophthora infestans)引起的马铃薯晚疫病是马铃薯的一种毁灭性病害,全世界普遍发生,危害严重,己列为世界粮食作物第一大病害。1950年中国许多地区晚疫病暴发,华北马铃薯当年减产30%-50%。上世纪60-70年代,由于引进抗病品种,晚疫病一度得到控制。但是从80年代起、晚疫病的流行频率和流行程度逐渐加重,又成为马铃薯生产的一个重要障碍,据估计, 展开更多
关键词 致病疫霉 PHYTOPHTHORA 抗性评价 克新 费乌瑞它 早大白 黄麻子 夏波蒂 流行程度 播种面积
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柑橘“黄龙病”之我见 预览
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作者 曹洪涛 《营销界:农资与市场》 2016年第18期75-76,共2页
近年来在南方柑橘产区,柑橘黄化现象极其严重,有很多的专家均鉴定为黄龙病。的确从各种症状上来看,均是黄龙病的症状,但是根据我在江西赣州等地的实际调查中却发现柑橘的黄化并非单纯的黄龙病所引起。经过两年的调查和研究,我个人认为... 近年来在南方柑橘产区,柑橘黄化现象极其严重,有很多的专家均鉴定为黄龙病。的确从各种症状上来看,均是黄龙病的症状,但是根据我在江西赣州等地的实际调查中却发现柑橘的黄化并非单纯的黄龙病所引起。经过两年的调查和研究,我个人认为当前的柑橘黄化发生的原因有以下三个方面:1.由柑橘木虱所传播的细菌引起的柑橘黄龙病;2.由柑橘线虫引起之柑橘萎凋病(或称黄叶病); 展开更多
关键词 黄龙病 柑橘黄 柑橘木虱 黄化现象 江西赣州 叶片黄化 黄叶病 PHYTOPHTHORA 水分管理 流胶病
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大豆疫霉MAPK基因的鉴定与转录分析 被引量:1
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作者 叶文武 李爱宁 +1 位作者 王晓莉 王源超 《植物病理学报》 CAS CSCD 北大核心 2016年第3期338-346,共9页
疫霉菌包括大豆疫霉等重要植物病原物,属于茸鞭生物界卵菌门,在进化上与真菌相差甚远;由于分离培养与遗传转化等相对困难,目前对其生长发育和致病机理的研究相对滞后。本研究综合运用基因组学和转录组学方法,首先对植物病原卵菌与真菌... 疫霉菌包括大豆疫霉等重要植物病原物,属于茸鞭生物界卵菌门,在进化上与真菌相差甚远;由于分离培养与遗传转化等相对困难,目前对其生长发育和致病机理的研究相对滞后。本研究综合运用基因组学和转录组学方法,首先对植物病原卵菌与真菌的蛋白激酶特别是MAPK进行了鉴定和比较,然后对大豆疫霉MAPK的基因结构、蛋白功能域以及转录模式等进行深入分析。结果表明,植物病原卵菌比真菌含有更多的蛋白激酶(包括MAPK),且卵菌及其近缘物种硅藻的MAPK与真菌在进化上相对独立;大豆疫霉的14个MAPK中,4个具有非典型的磷酸化唇序列,7个含有PH、C2、WW、PAS等与细胞信号转导相关的其它功能域;转录分析表明,大部分MAPK可能在大豆疫霉生长发育与致病的整个过程或特定过程中发挥重要作用。本文通过对蛋白激酶特别是MAPK的分析揭示了植物病原卵菌(与真菌相比)在细胞信号转导网络与机制上的独特性与复杂性,可为进一步研究疫霉菌MAPK的生物学功能及其信号调控机制提供参考。 展开更多
关键词 疫霉菌 基因组 蛋白激酶 功能域 转录分析
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