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A high?throughput three?dimensional cell culture platform for drug screening 认领
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作者 Jingyu Ock Wei Li 《生物设计与制造(英文)》 CSCD 2020年第1期40-47,共8页
Traditional two-dimensional(2D)cell cultures lack the extracellular matrix(ECM)-like structure or dynamic fluidic microenvironment for cells to maintain in vivo functionality.Three-dimensional(3D)tissue scaffolds,on t... Traditional two-dimensional(2D)cell cultures lack the extracellular matrix(ECM)-like structure or dynamic fluidic microenvironment for cells to maintain in vivo functionality.Three-dimensional(3D)tissue scaffolds,on the other hand,could provide the ECM-like microenvironment for cells to reformulate into tissue or organoids that are highly useful for in vitro drug screening.In this study,a high-throughput two-chamber 3D microscale tissue model platform is developed.Porous scaffolds are selectively foamed on a commercially available compact disk using laser.Perfusion of cell culture medium is achieved with centrifugal force-driven diffusion by disk rotation.Experimental studies were conducted on the fabrication process under various gas saturation and laser power conditions.Cell cultures were performed with two types of human cell lines:M059K and C3A-sub28.It is shown that the structure of microscale porous scaffolds can be controlled with laser foaming parameters and that coating with polydopamine these scaffolds are inducive for cell attachment and aggregation,forming a 3D network.With many such two-chamber models fabricated on a single CD and perfusion driven by the centrifugal force from rotation,the proposed platform provides a simple solution to the high-cost and lengthy drug development process with a high-throughput and physiologically more relevant tissue model system. 展开更多
关键词 HIGH-THROUGHPUT SCREENING Three-dimensional tissue model Drug SCREENING CENTRIFUGAL force-driven DIFFUSION Laser FOAMING
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Factors Associated with the Use of Cervical Cancer Screening in the Mbour Health District (Senegal) 认领
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作者 Niang Khadim Tine Jean Augustin Diegane +5 位作者 Thiam Ousmane Ly Toly Diongue Fatoumata Bintou Diallo Amadou Ibra Faye Adama Ndiaye Papa 《妇产科期刊(英文)》 2020年第4期604-614,共11页
Cervical cancer with an estimated incidence between 1700 and 2000 new cases per year and mortality around 70% to 80% has become the leading cancer in Senegal. The objectives of this present work were to study the fact... Cervical cancer with an estimated incidence between 1700 and 2000 new cases per year and mortality around 70% to 80% has become the leading cancer in Senegal. The objectives of this present work were to study the factors associated with cervical cancer screening in the Mbour health district in 2015. It was a cross-sectional, descriptive and analytical study carried out on a sample of 599 women chosen according to a two-stage survey. The survey used a questionnaire where data on individual characteristics, knowledge, attitudes and practices are collected during an individual interview after informed consent. The data collected were entered in the Epi Info 3.5.3 software and analyzed with the R 3.1.2 software. The average age was 44.4 years with a standard deviation of 8.9. The proportion of women screened for cervical cancer was 19.4%. Screening for cervical cancer was positively related to the possession of an income-generating activity (Adjusted OR = 2.39 [1.4 - 4.2]), increase of education and knowledge of a person suffering from cancer in the environment (adjusted OR = 2.40 [1.3 - 4.2]). Knowledge of the methods of diagnosis of cervical cancer was positively associated with cervical cancer screening (adjusted OR = 20 [11.1 - 33.3]). The lack of awareness and the lack of financial resources and education for women were the main obstacles to cervical cancer screening for women in Mbour. The health system should focus on building the capacity of women to fight cervical cancer. 展开更多
关键词 Cancer CERVIX SCREENING WOMEN Senegal
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Analysis of Screening Results of Four Common Infectious Diseases in One Stomatology Hospital 认领
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作者 Peng Sun Haili Yu +1 位作者 Meiling Yao Xiaolin Xu 《生物科学与医学(英文)》 2020年第4期96-102,共7页
Objective: To understand the status and characteristics of hepatitis B, hepatitis C, syphilis and HIV antibody screening in one dental hospital, and to provide scientific basis for the prevention and treatment of comm... Objective: To understand the status and characteristics of hepatitis B, hepatitis C, syphilis and HIV antibody screening in one dental hospital, and to provide scientific basis for the prevention and treatment of common infectious diseases in dental medical institutions. Methods: Collect all samples of patients screened for hepatitis B, hepatitis C, syphilis and HIV from one stomatological hospital in 2018-2019, and make statistics on the distribution of common infectious diseases among patients in stomatology, such as age, gender, type of treatment, department of treatment, etc. Results: The positive rate of hepatitis B was 2.27%, 39.42% in males and 60.58% in females. It was mainly detected by outpatient department, accounting for 87.5%. The age was concentrated in 30 - 60 years old, accounting for 77.88%. It was mainly distributed in oral and maxillofacial surgery, orthodontics and implant, accounting for 82.69%. The positive rate of hepatitis C was 0.1%, 33.33% in men and 66.67% in women. It was mainly detected by outpatient department, accounting for 83.33%. The age is concentrated in 20 - 50 years old, accounting for 100%. It is mainly distributed in oral and maxillofacial surgery, orthodontics and implant, accounting for 100%. The positive rate of syphilis was 0.18%, 33.33% for men and 66.67% for women. It was mainly detected by outpatient department, accounting for 83.33%. The age is concentrated in 30 - 50 years old, accounting for 100%. It was mainly distributed in oral and maxillofacial surgery, orthodontics and implant, accounting for 83.33%. HIV was not detected. Conclusion: The positive detection rate of common infectious diseases in the stomatological hospitals is generally lower than the average level. It is mainly distributed in the department of implant, oral and maxillofacial surgery and orthodontics. The highest positive detection rate of common infectious diseases is hepatitis B which is 2.27%, and the lowest is HIV which is 0. 展开更多
关键词 STOMATOLOGY INFECTIOUS DISEASES Screening
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Large Population Screening Identified the Main Risk Factors of Stroke in Shashi District of Jingzhou City 认领
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作者 Panpan Zheng Ling Zhang +1 位作者 Rui Sun Xiaoyan Peng 《长江医药(英文)》 2020年第1期70-78,共9页
Objective: This paper aims to screen and analyze the current status of high-risk stroke patients in Shashi District of Jingzhou City and the exposure levels of related risk factors, and provides suggestions as the ref... Objective: This paper aims to screen and analyze the current status of high-risk stroke patients in Shashi District of Jingzhou City and the exposure levels of related risk factors, and provides suggestions as the references for prevention and treatment of stroke. Methods: Using cluster sampling, on-site investigations were conducted on 1060 permanent residents aged 40 years and over at 3 townships and 2 communities in Shashi District of Jingzhou City from January 2018 to December 2018. Risk assessment of stroke is based on the stroke risk screening form. Statistical analysis was performed by using SPSS 22.0 software. Results: After making a stroke risk assessment, a total of 313 high-risk stroke patients were screened, and the detection rate was 29.53%. The exposure rate of risk factors from high to low was hypertension (70.93%), dyslipidemia (46.33%), less physical exercise (46.01%), diabetes (36.10%), overweight (33.55%), smoking (33.23%), family history of stroke (24.92%), atrial fibrillation or valvular heart disease (7.35%). There are statistically significant differences among all risk factors between the high-risk group and middle and low-risk groups (P Conclusion: The detection rate of high-risk stroke patients in Shashi District of Jingzhou City is high. Hypertension, dyslipidemia and less physical exercise are the main risk factors of stroke occurrence and recurrence in the region. The prevention and treatment of risk factors for stroke should be strengthened to control the incidence and recurrence rate of stroke. 展开更多
关键词 STROKE HIGH-RISK POPULATION SCREENING Risk Factors
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Phytochemical Screening and Antimicrobial Activity of <i>Desmodium ramosissimum</i> 认领
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作者 Badé Farid Toyigbénan Dah-Nouvlessounon Durand +10 位作者 Sina Haziz Nanoukon Chimène Moussè Wassiyath Nabéde Aklesso Assogba Sylvestre Tohoyéssou Majoie Nounagnon Martial Halfane Lehmane Adjanohoun Adolphe Savadogo Aly Baba-Moussa Lamine 《美国植物学期刊(英文)》 2020年第1期51-63,共13页
Microbial infections threaten the health of the Beninese population. Objectives: To identify bioactive phytochemical groups and evaluate the antimicrobial activity of Desmodium ramosissimum used in traditional medicin... Microbial infections threaten the health of the Beninese population. Objectives: To identify bioactive phytochemical groups and evaluate the antimicrobial activity of Desmodium ramosissimum used in traditional medicine in Benin against microbial infections. Methodology & Results: The classic reactions in solution helped highlight alkaloids, anthocyanins, flavonoids, quinones, saponins, steroids, tannins, terpenoids, mucilage, Coumarin, reducing compounds, anthracene, glycosides. The antibacterial activity was evaluated by extracts dilution’s method (ethanol extracts, methanol extracts and hexane extracts). The methanol extract inhibited the growth of all tested germs, with a significant effect on E. coli and S. aureus strains whose MIC values are equal to 1.25 mg/ml. On the contrary, this extract has a low activity on P. vulgaris strain with a MIC of 10 mg/ml. The ethanol extract also inhibited the growth of all the tested strains, but with more intense activity than the methanol extract. The strains of C. albicans and S. oralis were more sensitive to the extract with 0.625 mg/ml for MIC, while S. aureus and P. vulgaris were less sensitive (MIC = 5 mg/ml). The residual ethanol extract exerted inhibition on all microorganisms at a concentration of 20 mg/ml, except the strain of E. foecalis that was resistant. Conclusion & Applications: This study shows that besides its food virtues of this plant, there are antibacterial and antifungal properties. 展开更多
关键词 DESMODIUM BENIN EXTRACTS ANTIMICROBIAL Screening
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Cervical Length Estimation and Cervicovaginal Fluid for Placental <i>α</i>-Microglobulin 1 Testing to Screen Women Had Threatened Preterm Labor for Time till Spontaneous Labor 认领
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作者 Mohamed Kandil Abdelhaseib Salah Saad Alaa Masood 《生殖科学(英文)》 2020年第1期57-70,共14页
Objectives: Evaluation of diagnostic performance of rapid testing of cervico-vaginal fluid (CVF) for fetal fibronectin (FFN) and placental α-microglobulin 1 (PAMG-1) as screening of women presented by threatened pret... Objectives: Evaluation of diagnostic performance of rapid testing of cervico-vaginal fluid (CVF) for fetal fibronectin (FFN) and placental α-microglobulin 1 (PAMG-1) as screening of women presented by threatened preterm labor (TPTL) with intact membranes for duration till getting spontaneous labor (SL). Patients & Methods: 37 women presenting with TPTL underwent CVF samplings before digital examination and then underwent transvaginal ultrasonography for estimation of cervical length (CL). All women received fluid and anxiolytic therapy and if uterine contractions persisted, all had received tocolytic therapy with oral nifedipine or intravenous magnesium sulphate according to requirements. Incidence of SL within Results: Incidence of SL was 13.5%, 35.2% and 51.3% within 48-hr, 2 - 7 and 7 - 14 days, respectively. Duration till labor after sampling was positively correlated with CL, while was negatively correlated with positive FFN and PAMG-1 tests. Positive FFN test had high specificity, while positive PAMG-1 test had high sensitivity for labor within 7 days. Regression analysis defined short CL and positive PAMG-1 test as significant predictors for short duration till SL. ROC curve analysis defined short cervix and positive PAMG-1 test as significant predictors for labor within 48-hr and within 2 - 7 days respectively and combined negative PAMG-1 test and CL of 20 - 25 mm were significant predictors for labor within 7 - 14 days. Conclusion: PAMG-1 test had high specificity, if positive, for predicting SL and high NPP, if negative, for excluding labor within 7 days, so it can be used as rapid adjuvant to clinical evaluation to help management decision-making. Moreover, PAMG-1 test is recommended screening test for being easy-to-use bedside test, provides rapid results, can be used after vaginal exam and coitus and does not require a speculum examination or specialized equipment to analyze results. 展开更多
关键词 THREATENED Preterm LABOR Cervico-Vaginal Fluid Fetal Fibronectin PLACENTAL α-Microglobulin 1 Screening Spontaneous LABOR
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转基因油菜筛查阳性质粒分子的研制及应用 认领
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作者 李俊 李夏莹 +7 位作者 王颢潜 翟杉杉 陈子言 高鸿飞 李允静 吴刚 张秀杰 武玉花 《中国农业科学》 CAS CSCD 北大核心 2020年第7期1322-1337,共16页
【目的】转基因油菜是四大转基因作物之一,是中国转基因生物安全监管的重要对象,转基因检测为转基因安全监管提供技术支撑。转基因筛查是转基因检测的第一步,筛查靶标设置不合理会导致漏检部分转基因成分。建立转基因油菜筛查策略,并研... 【目的】转基因油菜是四大转基因作物之一,是中国转基因生物安全监管的重要对象,转基因检测为转基因安全监管提供技术支撑。转基因筛查是转基因检测的第一步,筛查靶标设置不合理会导致漏检部分转基因成分。建立转基因油菜筛查策略,并研制与筛查策略配套的阳性质粒分子,将为中国的转基因油菜安全监管提供强有力的技术支撑。【方法】通过收集数据库中登记的转基因油菜品种的外源基因元件信息,分析转基因油菜品种中常用的调控元件和标记基因,基于最大筛查覆盖率原则,确定转基因油菜的筛查靶标。通过检索数据库或查询专利,收集筛查元件的核苷酸序列。一个筛查元件通常有多个标准方法,查阅各筛查元件的检测标准,分析各标准中普通PCR引物对和实时荧光PCR引物/探针组合在筛查元件核苷酸序列中的位置,根据引物探针的结合位点,确定拟构建到质粒上的各筛查元件的核苷酸序列。人工合成各筛查元件和油菜内标基因的融合序列,克隆到常用质粒pUC18,构建阳性质粒分子。采用各筛查元件的普通PCR和实时荧光PCR方法,评估阳性质粒分子的适用性。【结果】建立了转基因油菜的筛查策略,通过检测CaMV 35S启动子、FMV 35S启动子、Bar、PAT、CP4-EPSPS、NPTⅡ、HPT、NOS终止子和PinⅡ终止子共9个基因元件,可实现已知信息转基因油菜品种的全覆盖。构建出聚合9个筛查元件和2个油菜内标基因HMG I/Y和CruA的转基因油菜筛查质粒分子pYCSC-1905。9个筛查元件和2个油菜内标基因的扩增效率均在90%—110%,证明质粒分子上的不同靶标序列没有相互干扰,影响PCR的扩增效率。质粒分子pYCSC-1905可用作9个筛查元件和2个油菜内标基因的通用阳性对照,适用于国家标准(GB/T和农业农村部公告)、出入境检验检疫行业标准(SN/T)和欧盟标准。【结论】提出的转基因油菜筛查策� 展开更多
关键词 转基因油菜 筛查 筛查策略 阳性质粒分子 应用
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粪便Vimentin和SFRP2甲基化在结直肠癌筛查中的作用 认领
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作者 韩冰 申玉翠 +1 位作者 王慧 张其胜 《胃肠病学和肝病学杂志》 CAS 2020年第5期561-566,共6页
目的探讨粪便中Vimentin和SFRP2甲基化状态在结直肠癌筛查中的价值。方法收集患者合格的粪便标本69例,其中结直肠癌23例、进展期腺瘤24例和健康人群22名,采用甲基化特异性PCR技术分析Vimentin和SFRP2甲基化状态,并与单个基因甲基化和粪... 目的探讨粪便中Vimentin和SFRP2甲基化状态在结直肠癌筛查中的价值。方法收集患者合格的粪便标本69例,其中结直肠癌23例、进展期腺瘤24例和健康人群22名,采用甲基化特异性PCR技术分析Vimentin和SFRP2甲基化状态,并与单个基因甲基化和粪便免疫隐血试验(FIT)的检测性能相比较,评价其在结直肠癌筛查中的灵敏度和特异度。结果结直肠癌组中单个Vimentin和SFRP2甲基化检出率分别为82.6%和69.6%,进展期腺瘤组为62.5%和41.7%,正常对照组为13.6%和13.6%。Vimentin和SFRP2联合检测在结直肠癌组的灵敏度为87.0%,高于FIT的56.5%(χ~2=5.25,P<0.05),与单基因检测比较,差异无统计学意义(P>0.05)。进展期腺瘤组中,联合检测的灵敏度为70.8%,高于SFRP2的41.7%(χ~2=4.15,P<0.05)和FIT的29.2%(χ~2=8.33,P<0.01),与Vimentin检测比较差异无统计学意义(P>0.05)。正常对照组联合检测的特异度为86.4%,与单基因检测相同,与FIT(72.7%)比较,差异无统计学意义(P>0.05)。联合检测在管状腺瘤中检出率为92.3%,高于SFRP2的53.8%(χ~2=4.9,P<0.05),与Vimentin(76.9%)比较,差异无统计学意义(P>0.05),在绒毛状管状腺瘤和管状腺瘤/绒毛状管状腺瘤中检出率与Vimentin相同,与SFRP2比较,差异无统计学意义(P>0.05)。联合检测在伴有上皮内瘤变中的检出率与单基因检测差异均无统计学意义(P>0.05)。结论粪便中联合Vimentin和SFRP2检测优于单基因及FIT检测,在结直肠癌筛查中具有潜在的应用价值。 展开更多
关键词 结直肠癌 VIMENTIN SFRP2 甲基化 粪便 筛查
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Screening and evaluation of reliable traits of upland cotton(Gossypium hirsutum L.)genotypes for salt tolerance at the seedling growth stage 认领
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作者 SIKDER Ripon Kumar WANG Xiangru +6 位作者 JIN Dingsha ZHANG Hengheng GUI Huiping DONG Qiang PANG Nianchang ZHANG Xiling SONG Meizhen 《棉花研究(英文)》 2020年第2期90-102,共13页
Background:Salt stress significantly inhibits the growth,development,and productivity of cotton because of osmotic,ionic,and oxidative stresses.Therefore,the screening and development of salt tolerant cotton cultivars... Background:Salt stress significantly inhibits the growth,development,and productivity of cotton because of osmotic,ionic,and oxidative stresses.Therefore,the screening and development of salt tolerant cotton cultivars is a key issue towards sustainable agriculture.This study subjected 11 upland cotton genotypes at the seedling growth stage to five different salt concentrations and evaluated their salt tolerance and reliable traits.Results:Several morpho-physiological traits were measured after 10 days of salinity treatment and the salt tolerance performance varied significantly among the tested cotton genotypes.The optimal Na Cl concentration for the evaluation of salt tolerance was 200 mmol·L-1.Membership function value and salt tolerance index were used to identify the most consistent salt tolerance traits.Leaf relative water content and photosynthesis were identified as reliable indicators for salt tolerance at the seedling stage.All considered traits related to salt tolerance indices were significantly and positively correlated with each other except for malondialdehyde.Cluster heat map analysis based on the morpho-physiological salt tolerance-indices clearly discriminated the 11 cotton genotypes into three different salt tolerance clusters.Cluster I represented the salt-tolerant genotypes(Z9807,Z0228,and Z7526)whereas clusters II(Z0710,Z7514,Z1910,and Z7516)and III(Z0102,Z7780,Z9648,and Z9612)represented moderately salttolerant and salt-sensitive genotypes,respectively.Conclusions:A hydroponic screening system was established.Leaf relative water content and photosynthesis were identified as two reliable traits that adequately represented the salt tolerance of cotton genotypes at the seedling growth stage.Furthermore,three salt-tolerant genotypes were identified,which might be used as genetic resources for the salt-tolerance breeding of cotton. 展开更多
关键词 Cotton genotypes Salt stress Screening Membership function value Cluster analysis
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串联质谱技术应用于新生儿遗传代谢病筛查,以甘肃省为例 认领
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作者 孙丽芳 李杰 《基因组学与应用生物学》 CAS CSCD 北大核心 2020年第4期1955-1960,共6页
为了明确甘肃省新生儿遗传代谢病的患病情况及分布情况。2017年1月1日至2018年10月31日期间,本研究应用串联质谱技术对41232例甘肃省新生儿进行遗传代谢病筛查。研究显示,所有筛查的新生儿中,共有67例确诊为遗传性代谢病,患病率为16.25/... 为了明确甘肃省新生儿遗传代谢病的患病情况及分布情况。2017年1月1日至2018年10月31日期间,本研究应用串联质谱技术对41232例甘肃省新生儿进行遗传代谢病筛查。研究显示,所有筛查的新生儿中,共有67例确诊为遗传性代谢病,患病率为16.25/10000。疾病类型主要包括3大类,即氨基酸代谢病(苯丙酮尿症,高甲硫氨酸血症,高苯丙氨酸血症)、有机酸代谢病(甲基丙二酸血症,β-酮硫解酶缺乏症,异戊酸血症)和脂肪酸代谢病(脂肪酸氧化代谢障碍、原发性肉碱缺乏症)。苯丙酮尿症的患病例数和患病率为最高,其次为甲基丙二酸血症。此外,阳性率和确诊率在不同性别、出生体重、出生季度和分娩方式亚组中无显著差异(p>0.05)。然而,在出生孕周亚组中,足月儿的阳性率和确诊率显著低于早产儿和过期产儿(p<0.05),提示孕周可能是遗传代谢病患病的重要影响因素。 展开更多
关键词 串联质谱 遗传代谢病 新生儿 筛查 孕周
海洋多黏类芽胞杆菌可湿性粉剂的研制 认领
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作者 陈茹 曹雪梅 +5 位作者 吴海霞 李欢 陈新元 暴增海 马桂珍 王军强 《植物保护》 CAS CSCD 北大核心 2020年第3期62-69,共8页
以海洋多黏类芽胞杆菌Paenibacillus polymyxa为研究对象,通过单因素室内试验和田间试验,确定了可湿性粉剂配方及其对甜瓜枯萎病的田间防效。结果表明,可湿性粉剂最佳配方为:以皂土为载体的菌粉70%,稳定剂海藻酸钠14%,湿润剂蔗糖脂肪酸... 以海洋多黏类芽胞杆菌Paenibacillus polymyxa为研究对象,通过单因素室内试验和田间试验,确定了可湿性粉剂配方及其对甜瓜枯萎病的田间防效。结果表明,可湿性粉剂最佳配方为:以皂土为载体的菌粉70%,稳定剂海藻酸钠14%,湿润剂蔗糖脂肪酸酯8%,分散剂聚乙烯醇8%。制得的可湿性粉剂活菌数为1.23×10^10cfu/g,含水量6%,润湿时间48 s,悬浮率75%,杂菌率0。采用7种不同方法防治甜瓜枯萎病,结果表明,可湿性粉剂对甜瓜枯萎病的综合防治效果高达84.9%。该制剂对甜瓜枯萎病具有良好防效,可作为防治甜瓜枯萎病的药剂在生产上推广应用。 展开更多
关键词 多黏类芽胞杆菌 可湿性粉剂 筛选 田间防治
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孤独症早期预警征象与干预效果研究 认领
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作者 李琳 赵虹 《中国初级卫生保健》 2020年第4期37-40,共4页
目的通过对1岁以内存在早期孤独症预警征象儿童进行跟踪随访及家庭干预,探讨其与存在临床表现但未进行家庭干预儿童的孤独症筛查结果及与智能发育之间的关系。方法采用《儿童孤独症诊疗康复指南》筛查出1岁以内存在预警征象的儿童(实验... 目的通过对1岁以内存在早期孤独症预警征象儿童进行跟踪随访及家庭干预,探讨其与存在临床表现但未进行家庭干预儿童的孤独症筛查结果及与智能发育之间的关系。方法采用《儿童孤独症诊疗康复指南》筛查出1岁以内存在预警征象的儿童(实验组)和18~24月存在孤独症临床表现的儿童(对照组),在18~24月进行CHAT-23量表和儿心量表进行评估,对结果进行对比分析。结果实验组CHAT-23量表筛查结果可疑和异常人数均明显低于对照组,两组存在显著差异(P<0.05);两组患儿总发育商均低于正常儿童,但以对照组儿童落后明显,尤以语言和社会交往落后明显,各能区之间除大运动、语言两组无显著差异外(P>0.05),其余各组均有显著差异(P<0.05);CHAT-23量表两组儿童单项阳性率比较,发现多组项目存在显著差异(P<0.05),且7项核心项目均存在显著差异。结论早期筛查存在孤独症预警征象儿童,早期家庭干预,能在一定程度是改善孤独症儿童的预后,并提高其智能发育水平。 展开更多
关键词 孤独症 早期预警征象 筛查 发育商
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筛查宫颈癌疾病方法的现状与研究进展 认领 被引量:1
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作者 陈珅 《中国城乡企业卫生》 2020年第2期46-48,共3页
宫颈癌是女性群体的高发疾病之一,其发病率位居乳腺癌之后,是威胁女性健康的重大疾病。有数据显示,该病每年的新发病例约为47万人,且在发展中国家的发病率约占80%。在我国每年约有10万人次女性患宫颈癌。为改善患者的预后,临床认为,应... 宫颈癌是女性群体的高发疾病之一,其发病率位居乳腺癌之后,是威胁女性健康的重大疾病。有数据显示,该病每年的新发病例约为47万人,且在发展中国家的发病率约占80%。在我国每年约有10万人次女性患宫颈癌。为改善患者的预后,临床认为,应尽早诊断并治疗宫颈癌。其常规诊断方法为巴氏涂片,可筛查出早期癌变,进而指导治疗。但其取材或制片等过程中存在较多问题,假阴性几率较高。基于此,相关学者研发出薄层细胞学检查技术(TCT),具有较高的敏感性与特异性,且能够重复操作。肉眼观察(VIA)筛查该病的准确性较高,被大部分患者所接受,但其敏感性相对较低,可广泛用于发展中国家或经济较落后的部分地区。现阶段,人乳头瘤病毒(HPV)检测与分子标志物等成为该病的新型筛查方法,其检出率与安全性高。本研究将全面阐述该病的常见筛查方法,以指导疾病筛查实践。 展开更多
关键词 宫颈癌 筛查 细胞学检测 分子标志物 人乳头瘤病毒检测
社区糖尿病患者大肠癌筛查结果分析 认领
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作者 杨军花 张玮 +5 位作者 杨安卿 秦征魏 刘岩 花云 华红梅 郭稀 《中国全科医学》 CAS 北大核心 2020年第6期662-666,共5页
背景随着现代生活方式的改变,糖尿病和大肠癌发病率均呈逐渐上升趋势。糖尿病与大肠癌的相关性研究证明,糖尿病是大肠癌的高危因素,该人群应定期进行大肠癌筛查。目的分析糖尿病患者参与大肠癌筛查的结果,为今后社区糖尿病慢性病管理工... 背景随着现代生活方式的改变,糖尿病和大肠癌发病率均呈逐渐上升趋势。糖尿病与大肠癌的相关性研究证明,糖尿病是大肠癌的高危因素,该人群应定期进行大肠癌筛查。目的分析糖尿病患者参与大肠癌筛查的结果,为今后社区糖尿病慢性病管理工作提供更多的技术参考。方法选取2016-2018年上海市白玉社区卫生服务中心所辖社区中参与大肠癌筛查的糖尿病患者319例,研究初期发放两份调查问卷表,一份是基于体检要求本中心自行设计的健康体检表,主要调查参与筛查的居民有无确诊糖尿病史。另一份为上海市大肠癌筛查项目组统一编制的《上海市社区居民大肠癌筛查危险度评估表》,内容包括基本情况(性别、年龄、婚姻状况、文化程度、职业)、大肠癌危险因素(慢性腹泻史、慢性便秘史、黏液血便史、慢性阑尾炎或阑尾切除史、慢性胆囊炎或胆囊切除史、肠息肉史、血吸虫病史、癌症史、一级亲属肿瘤史、精神创伤史),记录初筛结果(便隐血试验阳性、危险度评估阳性,任一阳性为初筛阳性)。研究后期针对初筛阳性者发放本中心自行编制的调查问卷表,内容包括是否行肠镜检查、肠镜检查结果、对肠镜检查相关认知(对肠镜检查害怕、认为自己不会有问题、担心检查后出现不适后果、担心查出问题有心理负担、没时间没兴趣、肠镜检查有益处)。结果 319例患者年龄以60~69岁居多(45.8%),婚姻状况以已婚为主(91.2%),文化程度以中专/中学为主(57.1%),职业以国有企业为主(39.8%)。大肠癌危险因素以慢性阑尾炎或阑尾切除史、肠息肉史、慢性胆囊炎或胆囊切除史占比较高,分别为14.7%、13.8%、13.5%。便隐血试验阳性率6.6%(21/319),危险度评估阳性率18.2%(58/319),双阳性率6.0%(19/319)。79例初筛阳性患者中,有23例进行了肠镜检查,其中无病变者占39.1%、肠炎者占26.1%、息肉 展开更多
关键词 糖尿病 肠肿瘤 社区卫生中心 筛查
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重复实施结直肠癌筛查服务的效率分析 认领
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作者 吴毅凌 杨鹏 +4 位作者 李志媛 褚秀娟 罗炜 苏旭燕 郎冬晨 《中国肿瘤》 CAS CSCD 北大核心 2020年第3期172-176,共5页
[目的]了解重复开展结直肠癌筛查的成效。[方法]以2015—2017年上海市松江区参加结直肠癌筛查的居民为研究对象,比较不同筛查服务次数发现癌前病变和结直肠癌的情况。[结果]2015—2017年上海市松江区共完成结直肠癌筛查199456人次,涉及... [目的]了解重复开展结直肠癌筛查的成效。[方法]以2015—2017年上海市松江区参加结直肠癌筛查的居民为研究对象,比较不同筛查服务次数发现癌前病变和结直肠癌的情况。[结果]2015—2017年上海市松江区共完成结直肠癌筛查199456人次,涉及居民137715人,发现癌前期病变4147人,结直肠癌201人。34.16%的调查对象3年中参与了多次筛查。一次筛查完成初筛137715人次,实施肠镜检查10022人次,癌前病变初筛检出率为2070.94/10万,肠镜检出率为28.46%,结直肠癌初筛检出率为94.40/10万,肠镜检出率为1.30%;二次筛查完成初筛47045人次,实施肠镜检查4142人次,癌前病变初筛检出率为2442.34/10万,肠镜检出率为27.74%,结直肠癌初筛检出率为108.41/10万,肠镜检出率为1.23%;三次筛查完成初筛14696人次,实施肠镜检查2141人次,癌前病变初筛检出率为4191.62/10万,肠镜检出率为28.77%,结直肠癌初筛检出率为149.70/10万,肠镜检出率为1.03%。二次、三次筛查的癌前病变初筛检出率高于一次筛查,癌前病变及结直肠癌的肠镜检出率差异无统计学意义。[结论]3年内多次实施结直肠癌筛查的效率与一次筛查相近,应提高居民肠镜检查依从性,可对60岁以上男性居民提供多次筛查服务从而提高筛查效益。 展开更多
关键词 结直肠癌 筛查 效率 肠镜 上海
依据《中小学生屈光不正筛查规范》开展青少年屈光不正防控工作 认领
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作者 邹海东 何鲜桂 +3 位作者 马莹琰 马军 董彬 许迅 《中华实验眼科杂志》 CAS CSCD 北大核心 2020年第6期473-475,共3页
屈光不正性视力损害已成为我国乃至全球范围内的公共卫生问题,儿童及青少年的近视防控工作已引起我国政府的高度重视,建立目标人群的视力健康档案、开展屈光不正的筛查是儿童及青少年近视防控工作的重要环节和有效措施。长期以来,由于... 屈光不正性视力损害已成为我国乃至全球范围内的公共卫生问题,儿童及青少年的近视防控工作已引起我国政府的高度重视,建立目标人群的视力健康档案、开展屈光不正的筛查是儿童及青少年近视防控工作的重要环节和有效措施。长期以来,由于国内外均缺乏相关的筛查标准,我国在相关筛查工作中存在开展筛查工作的主体资质和管理混乱、筛查指标欠合理及其界值不统一、筛查质量控制不佳、操作方法不规范和结果反馈不科学等问题。在国家卫生健康委员会的推动和支持下,推荐性国家卫生行业标准WS/T 663-2019《中小学生屈光不正筛查规范》于2020年1月11日正式发布并定于2020年6月1日正式实施,为我国规范性开展儿童及青少年的屈光不正筛查工作提供技术标准和指导意见。依据《中小学生屈光不正筛查规范》积极开展儿童及青少年的屈光不正筛查和防控是现阶段我国各级医疗和教育机构的重要任务和责任。 展开更多
关键词 屈光不正 中小学生 筛查 近视防控 规范
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出生缺陷三级预防的实验室检查方法探讨 认领
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作者 汪竟成 黄仲军 尹耕心 《国际检验医学杂志》 CAS 2020年第S01期162-166,共5页
出生缺陷有3个阶段的预防,分别是孕前、孕期和新生儿期的一、二、三级预防,本文简述出生缺陷病谱和一级预防的相关实验室检查,主要叙述和讨论了串联质谱技术在二、三级预防中用于遗传代谢病的实验室检查方法学,以实验室检查角度为出生... 出生缺陷有3个阶段的预防,分别是孕前、孕期和新生儿期的一、二、三级预防,本文简述出生缺陷病谱和一级预防的相关实验室检查,主要叙述和讨论了串联质谱技术在二、三级预防中用于遗传代谢病的实验室检查方法学,以实验室检查角度为出生缺陷的三级预防工作提供一点技术参考。 展开更多
关键词 出生缺陷 筛查 串联质谱技术 遗传代谢病
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In Silico Screening of Potential Spike Glycoprotein Inhibitors of SARS-CoV-2 with Drug Repurposing Strategy 认领
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作者 WEI Tian-zi WANG Hao +9 位作者 WU Xue-qing LU Yi GUAN Sheng-hui DONG Feng-quan DONG Chen-le ZHU Gu-li BAO Yu-zhou ZHANG Jian WANG Guan-yu LI Hai-ying 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2020年第9期663-669,共7页
Objective:To select potential molecules that can target viral spike proteins,which may potentially interrupt the interaction between the human angiotension-converting enzyme 2(ACE2)receptor and viral spike protein by ... Objective:To select potential molecules that can target viral spike proteins,which may potentially interrupt the interaction between the human angiotension-converting enzyme 2(ACE2)receptor and viral spike protein by virtual screening.Methods:The three-dimensional(3D)-coordinate file of the receptor-binding domain(RBD)-ACE2 complex for searching a suitable docking pocket was firstly downloaded and prepared.Secondly,approximately 15,000 molecular candidates were prepared,including US Food and Drug Administration(FDA)-approved drngs from DrugBank and natural compounds from Traditional Chinese Medicine Systems Pharmacology(TCMSP),for the docking process.Then,virtual screening was performed and the binding energy in Autodock Vina was calculated.Finally,the top 20 molecules with high binding energy and their Chinese medicine(CM)herb sources were listed in this paper.Results:It was found that digitoxin,a cardiac glycoside in DrugBank and bisindigotin in TCMSP had the highest docking scores.Interestingly,two of the CM herbs containing the natural compounds that had relatively high binding scores,Forsyfh/ae frucft/s and/saf/d/s racWx,are components of Lianhua Qingwen(莲花清痕),a CM formula reportedly exerting activity against severe acute respiratory syndrome(SARS)-Cov-2.Moreover,raltegravir,an HIV integrase inhibitor,was found to have a relatively high binding score.Conclusions:A class of compounds,which are from FDA-approved drugs and CM natural compounds,that had high binding energy with RBD of the viral spike protein.Our work provides potential candidates for other researchers to identify inhibitors to prevent SARS-CoV-2 infection,and highlights the importance of CM and integrative application of CM and Western medicine on treating COVID-19. 展开更多
关键词 COVID-19 SARS-CoV-2 drug repurposing virtual screening Chinese medicine
妊娠早期糖化血红蛋白检测联合体质量指数筛查妊娠期糖尿病的临床价值 认领
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作者 王冬玲 黄燕萍 吴川平 《海南医学》 CAS 2020年第6期754-756,共3页
目的 探讨妊娠早期糖化血红蛋白(HbA1c)检测联合体质量指数(BMI)在妊娠期糖尿病筛查中的应用价值.方法 回顾性分析2017年1月至2018年12月在连州市妇幼保健计划生育服务中心妇产科进行建档、产前常规检查,并行分娩的1 100例单胎孕妇的临... 目的 探讨妊娠早期糖化血红蛋白(HbA1c)检测联合体质量指数(BMI)在妊娠期糖尿病筛查中的应用价值.方法 回顾性分析2017年1月至2018年12月在连州市妇幼保健计划生育服务中心妇产科进行建档、产前常规检查,并行分娩的1 100例单胎孕妇的临床资料,在孕8~14周进行HbA1c及BMI筛查.以孕24~28周进行75 g葡萄糖耐量试验(OGTT)结果 作为诊断标准,观察筛查结果 ,将糖代谢异常的74例孕妇作为观察组,同时随机选取74例正常孕妇作为对照组,比较两组孕妇的HbA1c及BMI水平.结果 观察组孕妇HbA1c及BMI分别为(9.28±0.85)%、(25.64±1.37)kg/m2,明显高于对照组的(4.52±0.76)%、(21.87±1.24)kg/m2,差异均有统计学意义(P<0.05);以各种筛查方法 综合比较分析,HbA1c≥5.5%且BMI≥24的筛查方法 诊断妊娠期糖尿病的敏感性为89.19%,特异性为89.91%.结论 妊娠期糖尿病孕妇的糖化血红蛋白水平及体质量指数显著高于正常孕妇,糖化血红蛋白及体质量指数检测在孕早期应用于妊娠期糖尿病的筛查具有较高的敏感性、特异性,对妊娠期糖尿病的筛查具有临床应用价值. 展开更多
关键词 妊娠期糖尿病 糖化血红蛋白 体质量指数 筛查 应用价值
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大同地区马铃薯抗旱品种比较试验 认领
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作者 杜培兵 白小东 +5 位作者 范向斌 杨春 齐海英 王兴涛 毛向红 朱智慧 《农业技术与装备》 2020年第6期9-10,12,共3页
2017年—2018年山西省农业科学院高寒区作物研究所在东王庄试验基地旱棚利用盆栽控水法试验筛选出6个抗旱马铃薯品种:中薯19号、同薯23号、晋薯16号、晋24号、青薯9号和大同里外黄。文章对这6个品种进行大田对比试验,对马铃薯的物期、... 2017年—2018年山西省农业科学院高寒区作物研究所在东王庄试验基地旱棚利用盆栽控水法试验筛选出6个抗旱马铃薯品种:中薯19号、同薯23号、晋薯16号、晋24号、青薯9号和大同里外黄。文章对这6个品种进行大田对比试验,对马铃薯的物期、植株形态和发病率调查,对各品种薯块品质分析测定,对块茎商品薯率和产量的比较。结果表明:这6个品种性状优良,适宜在大同干旱地区种植推广。 展开更多
关键词 马铃薯 抗旱 抗病 筛选
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