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Transplantation of adult spinal cord grafts into spinal cord transected rats improves their locomotor function
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作者 He Shen Xi Chen +3 位作者 Xing Li Ke Jia Zhifeng Xiao Jianwu Dai 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第6期725-733,共9页
Grafted embryonic central neural tissue pieces can recover function of hemisected spinal cord in neonatal rats and promote axonal growth in adults. However, spinal cord segments from adults have not been used as donor... Grafted embryonic central neural tissue pieces can recover function of hemisected spinal cord in neonatal rats and promote axonal growth in adults. However, spinal cord segments from adults have not been used as donor segments for allogeneic transplantation. Here, we utilized adult spinal cord tissue grafts(aSCGs) as donor constructs for repairing complete spinal cord injury(SCI). Moreover, to provide a favourable microenvironment for SCI treatment, a growth factor cocktail containing three growth factors(brain-derived neurotrophic factor, neurotrophin-3 and vascular endothelial growth factor), was applied to the aSCG transplants. We found that the locomotor function was significantly improved 12 weeks after transplantation of aSCGs into the spinal cord lesion site in adult rats. Transplantation of aSCGs combined with these growth factors enhanced neuron and oligodendrocyte survival and functional restoration. These encouraging results indicate that treatment of complete SCI by transplanting aSCGs, especially in the presence of growth factors, has a positive effect on motor functional recovery, and therefore could be considered as a possible therapeutic strategy for SCI. 展开更多
关键词 SPINAL CORD injury (SCI) TRANSPLANTATION ADULT SPINAL CORD GRAFTS (aSCGs) function recovery ADULT host transection
Use of ebselen as a neuroprotective agent in rat spinal cord subjected to traumatic injury 预览
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作者 Wojciech Susarczyk Edyta Olakowska +5 位作者 Magdalena Larysz-Brysz Izabella Woszczycka-Korczyńska Daria Gendosz de Carrillo Wladyslaw P.Weglarz Joanna Lewin-Kowalik Wieslaw Marcol 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1255-1261,共7页
Spinal cord injury(SCI)causes disturbances of motor skills.Free radicals have been shown to be essential for the development of spinal cord trauma.Despite some progress,until now no effective pharmacological therapies... Spinal cord injury(SCI)causes disturbances of motor skills.Free radicals have been shown to be essential for the development of spinal cord trauma.Despite some progress,until now no effective pharmacological therapies against SCI have been verified.The purpose of our experiment was to investigate the neuroprotective effects of ebselen on experimental SCI.Twenty-two rats subjected to SCI were randomly subjected to SCI with no further treatment(n=10)or intragastric administration of ebselen(10 mg/kg)immediately and 24 hours after SCI.Behavioral changes were assessed using the Basso,Beattie,and Bresnahan locomotor scale and footprint test during 12 weeks after SCI.Histopathological and immunohistochemical analyses of spinal cords and brains were performed at 12 weeks after SCI.Magnetic resonance imaging analysis of spinal cords was also performed at 12 weeks after SCI.Rats treated with ebselen presented only limited neurobehavioral progress as well as reduced spinal cord injuries compared with the control group,namely length of lesions(cysts/scars)visualized histopathologically in the spinal cord sections was less but cavity area was very similar.The same pattern was found in T2-weighted magnetic resonance images(cavities)and diffusion-weighted images(scars).The number of FluoroGold retrogradely labeled neurons in brain stem and motor cortex was several-fold higher in ebselen-treated rats than in the control group.The findings suggest that ebselen has only limited neuroprotective effects on injured spinal cord.All exprimental procedures were approved by the Local Animal Ethics Committee for Experiments on Animals in Katowice(Katowice,Poland)(approval No.19/2009). 展开更多
关键词 EBSELEN ANTIOXIDANT NEUROPROTECTION NEUROREGENERATION SPINAL CORD injury SPINAL CORD repair functional recovery
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Spinal Cord Stimulation for Pain Treatment After Spinal Cord Injury
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作者 Qian Huang Wanru Duan +6 位作者 Eellan Sivanesan Shuguang Liu Fei Yang Zhiyong Chen Neil C.Ford Xueming Chen Yun Guan 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第3期527-539,共13页
In addition to restoration of bladder, bowel, and motor functions, alleviating the accompanying debilitating pain is equally important for improving the quality of life of patients with spinal cord injury(SCI). Curren... In addition to restoration of bladder, bowel, and motor functions, alleviating the accompanying debilitating pain is equally important for improving the quality of life of patients with spinal cord injury(SCI). Currently,however, the treatment of chronic pain after SCI remains a largely unmet need. Electrical spinal cord stimulation(SCS) has been used to manage a variety of chronic pain conditions that are refractory to pharmacotherapy. Yet, its efficacy, benefit profiles, and mechanisms of action in SCI pain remain elusive, due to limited research, methodological weaknesses in previous clinical studies, and a lack of mechanistic exploration of SCS for SCI pain control. We aim to review recent studies and outline the therapeutic potential of different SCS paradigms for traumatic SCI pain. We begin with an overview of its manifestations,classification, potential underlying etiology, and currentchallenges for its treatment. The clinical evidence for using SCS in SCI pain is then reviewed. Finally, future perspectives of pre-clinical research and clinical study of SCS for SCI pain treatment are discussed. 展开更多
关键词 Pain TRAUMA SPINAL CORD injury SPINAL CORD stimulation NEUROMODULATION ANALGESIA
Micromechanical adaptation as a treatment for spinal cord injury 预览
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作者 Veronica Estrada Hans Werner Muller 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1909-1911,共3页
Spinal cord injury: Thus far injury of the spinal cord is incurable and, in the majority of cases, a devastating and life-changing event. The worldwide incidence rate of spinal cord injury (SCI) ranges from 250,000 to... Spinal cord injury: Thus far injury of the spinal cord is incurable and, in the majority of cases, a devastating and life-changing event. The worldwide incidence rate of spinal cord injury (SCI) ranges from 250,000 to 900,000 (www.who.int, 2013;Kumar et al., 2018) new cases per year. SCI outcome includes the damage of axons, demyelination of axons, loss of signal transduction, and consequential long-lasting motor and sensory deficits. Additionally, the non-use of muscles can lead to atrophy and joint contractures, thereby further reducing the possibility of recovery. Depending on the spinal level and the severity of the injury, the extent of the damage can vary and spontaneous recovery is possible to varying degrees. 展开更多
关键词 MICROMECHANICAL ADAPTATION SPINAL CORD INJURY SPINAL CORD injury(SCI)
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Use of longitudinal magnetic resonance imaging in preclinical models of spinal cord injury 预览
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作者 Harun Najib Noristani Florence Evelyne Perrin 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期771-772,共2页
Spinal cord injury(SCI)is a distressing event with grave socio/economic consequences to our society.Pathophysiological response following SCI involves blood-spinal cord barrier breakdown,neuroinflammation and formatio... Spinal cord injury(SCI)is a distressing event with grave socio/economic consequences to our society.Pathophysiological response following SCI involves blood-spinal cord barrier breakdown,neuroinflammation and formation of a glial scar that altogether govern the feasibility of spontaneous axonal re-growth and limited functional recovery.Great advances in understanding SCI pathophysiology have been achieved using numerous transgenic mouse lines developed in different strains.However,there are inherent strain differences that affect inflammation,gliosis,axon regeneration and ultimately functional recovery after SCI. 展开更多
关键词 SPINAL CORD injury(SCI) SPINAL CORD INJURY
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CNB-001 reduces paraplegia in rabbits following spinal cord ischemia 预览
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作者 Paul A. Lapchak Paul D. Boitano +5 位作者 Rene Bombien Daisy Chou Margot Knight Anja Muehle Mihaela Te Winkel Ali Khoynezhad 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2192-2198,共7页
aortic aneurysm repair and thoracic endovascular aortic repair results in devastating clinical deficits in patients. Because spinal cord ischemia is inadequately treated, we studied the effects of [4-((1E)-2-(5-(4-hyd... aortic aneurysm repair and thoracic endovascular aortic repair results in devastating clinical deficits in patients. Because spinal cord ischemia is inadequately treated, we studied the effects of [4-((1E)-2-(5-(4-hydroxy-3-methoxystyryl-)-1-phenyl-1H-pyrazoyl-3-yl) vinyl)-2-methoxy-phenol)](CNB-001), a novel curcumin-based compound, in a rabbit SCI model. CNB-001 is known to inhibit human 5-lipoxygenase and 15-lipoxygenase and reduce the ischemia-induced inflammatory response. Moreover, CNB- 001 can reduce the level of oxidative stress markers and potentiate brain-derived neurotrophic factor and brain-derived neurotrophic factor receptor signaling. The Tarlov scale and quantal analysis technique results revealed that CNB-001 administered as an intravenous dose (bolus) 30 minutes prior to spinal cord ischemia improved the behaviors of female New Zealand White rabbits. The improvements were similar to those produced by the uncompetitive N-methyl-D-aspartate receptor antagonist memantine. At 48 hours after aortic occlusion, there was a 42.7% increase (P < 0.05) in tolerated ischemia duration (n = 14) for rabbits treated with CNB-001 (n = 16), and a 72.3% increase for rabbits treated with the positive control memantine (P < 0.05)(n = 23) compared to vehicle-treated ischemic rabbits (n = 22). CNB-001 is a potential important novel treatment for spinal cord ischemia induced by aortic occlusion. All experiments were approved by the CSMC Institutional Animal Care and Use Committee (IACUC #4311) on November 1, 2012. 展开更多
关键词 curcumin analog SPINAL CORD injury SPINAL CORD ischemia thoraco-abdominal AORTIC ANEURYSM thoracic ENDOVASCULAR AORTIC repair motor function neuroprotection neurorepair
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Establishment and verification of a surgical prognostic model for cervical spinal cord injury without radiological abnormality 预览
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作者 Jie Wang Shuai Guo +2 位作者 Xuan Cai Jia-Wei Xu Hao-Peng Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期713-720,共8页
Some studies have suggested that early surgical treatment can effectively improve the prognosis of cervical spinal cord injury without radiological abnormality,but no research has focused on the development of a progn... Some studies have suggested that early surgical treatment can effectively improve the prognosis of cervical spinal cord injury without radiological abnormality,but no research has focused on the development of a prognostic model of cervical spinal cord injury without radiological abnormality.This retrospective analysis included 43 patients with cervical spinal cord injury without radiological abnormality.Seven potential factors were assessed:age,sex,external force strength causing damage,duration of disease,degree of cervical spinal stenosis,Japanese Orthopaedic Association score,and physiological cervical curvature.A model was established using multiple binary logistic regression analysis.The model was evaluated by concordant profiling and the area under the receiver operating characteristic curve.Bootstrapping was used for internal validation.The prognostic model was as follows:logit(P)=-25.4545+21.2576VALUE+1.2160SCORE-3.4224TIME,where VALUE refers to the Pavlov ratio indicating the extent of cervical spinal stenosis,SCORE refers to the Japanese Orthopaedic Association score(0–17)after the operation,and TIME refers to the disease duration(from injury to operation).The area under the receiver operating characteristic curve for all patients was 0.8941(95%confidence interval,0.7930–0.9952).Three factors assessed in the predictive model were associated with patient outcomes:a great extent of cervical stenosis,a poor preoperative neurological status,and a long disease duration.These three factors could worsen patient outcomes.Moreover,the disease prognosis was considered good when logit(P)≥-2.5105.Overall,the model displayed a certain clinical value.This study was approved by the Biomedical Ethics Committee of the Second Affiliated Hospital of Xi’an Jiaotong University,China(approval number:2018063)on May 8,2018. 展开更多
关键词 nerve REGENERATION SURGICAL prognostic model CERVICAL SPINAL cord injury retrospective study MULTIPLE binary logistic regression analysis BOOTSTRAPPING internal validation MULTIPLE imputations CERVICAL SPINAL stenosis duration of disease Pavlov ratio neural REGENERATION
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Effects of chondro-itinrase ABC combined with Jisuikang on neurological recovery and TGF-β1, HIF-1α and Nog-oNgR signaling pathways after spinal cord injury in rats
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作者 Yi-Ze Huang Lin Peng Ge Zhang 《海南医科大学学报(英文版)》 2019年第2期15-19,共5页
Objective:To explore the effects of chondroitinase ABC (Ch ABC) combined with spinal cord on neurological recovery and TGF-β1, HIF-1 and Nog-oNgR signaling pathways after spinal cord injury in rats.Methods: Sixty rat... Objective:To explore the effects of chondroitinase ABC (Ch ABC) combined with spinal cord on neurological recovery and TGF-β1, HIF-1 and Nog-oNgR signaling pathways after spinal cord injury in rats.Methods: Sixty rats were randomly divided into 4 groups: control group, model group, Ch ABC group and Ch ABC+ spinal cord. A rat spinal cord injury model was established using the Allen's method. Spinal nerve function was assessed by Basso Beattie Bresnahan (score) and somatosensory evoked potential (sEP) assays. mRNA levels were detected using RT-qPCR. Western blot was used to detect protein levels.Results: After modeling, the BBB scores of the rats were significantly decreased. After the intervention, the BBB scores of the three groups were improved. The BBB scores of the Ch ABC+ spinal cord group were significantly higher than those of the Ch ABC group. After modeling, the SEP of the rats was significantly increased. After the intervention, the BBB scores of the three groups were decreased. The BBB scores of the Ch ABC+ spinal cord group were significantly lower than those of the Ch ABC group. The TGF-β1 in the Ch ABC group and the Ch ABC+ spinal group was significantly higher than that in the model group, and the HIF-1 was significantly lower than the model group. The level of TGF-β1 in Ch ABC+ spinal cord group was significantly higher than that in Ch ABC group and HIF-1 was significantly lower than that in Ch ABC group. After treatment, the expression levels of Nog-oNgR pathway in Ch ABC group and Ch ABC + spinal cord group were significantly lower than those in model group, and the expression levels of Nogo-A, NgR and LINGO-1 in Ch ABC+ spinal cord group were significant after intervention. Lower than Ch ABC group.Conclusion: ChABC combined with spinal cord has a stronger role in promoting the recovery of neurological function. The combination of spinal cord can further inhibit the level of HIF-1 and increase the level of TGF-β1, and improve the prognosis to promote spinal healing. And the mechanism of actio 展开更多
关键词 SPINAL CORD INJURY SPINAL INJURY Chondro-itinrase ABC JISUIKANG
Dynamic changes in intramedullary pressure 72 hours after spinal cord injury 预览
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作者 Xin Zhang Chang-Bin Liu +9 位作者 De-Gang Yang Chuan Qin Xue-Chao Dong Da-Peng Li Chao Zhang Yun Guo Liang-Jie Du Feng Gao Ming-Liang Yang Jian-Jun Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期886-895,共10页
Intramedullary pressure increases after spinal cord injury,and this can be an important factor for secondary spinal cord injury.Until now there have been no studies of the dynamic changes of intramedullary pressure af... Intramedullary pressure increases after spinal cord injury,and this can be an important factor for secondary spinal cord injury.Until now there have been no studies of the dynamic changes of intramedullary pressure after spinal cord injury.In this study,telemetry systems were used to observe changes in intramedullary pressure in the 72 hours following spinal cord injury to explore its pathological mechanisms.Spinal cord injury was induced using an aneurysm clip at T10 of the spinal cord of 30 Japanese white rabbits,while another 32 animals were only subjected to laminectomy.The feasibility of this measurement was assessed.Intramedullary pressure was monitored in anesthetized and conscious animals.The dynamic changes of intramedullary pressure after spinal cord injury were divided into three stages:stage I(steep rise)1–7 hours,stage II(steady rise)8–38 hours,and stageⅢ(descending)39–72 hours.Blood-spinal barrier permeability,edema,hemorrhage,and histological results in the 72 hours following spinal cord injury were evaluated according to intramedullary pressure changes.We found that spinal cord hemorrhage was most severe at 1 hour post-spinal cord injury and then gradually decreased;albumin and aquaporin 4 immunoreactivities first increased and then decreased,peaking at 38 hours.These results confirm that severe bleeding in spinal cord tissue is the main cause of the sharp increase in intramedullary pressure in early spinal cord injury.Spinal cord edema and blood-spinal barrier destruction are important factors influencing intramedullary pressure in stagesⅡandⅢof spinal cord injury. 展开更多
关键词 nerve REGENERATION secondary SPINAL cord injury TELEMETRY PATHOLOGICAL mechanism rabbit CONSCIOUS anesthetized hemorrhage EDEMA pressure measurement blood-spinal barrier neural REGENERATION
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Clinical effect of combined anterior and posterior approach surgery for cervical spondylotic myelopathy 预览
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作者 Yanzhou Jiang Lianchong Fu Yunshan Mu 《临床研究与医学报告》 2019年第1期21-24,共4页
Objective: To evaluate the clinical efficacy of combined anterior and posterior approach surgery for patients with spinal type. Methods: Randomly selected from January 2013 to December 2015 in our hospital 96 cases of... Objective: To evaluate the clinical efficacy of combined anterior and posterior approach surgery for patients with spinal type. Methods: Randomly selected from January 2013 to December 2015 in our hospital 96 cases of cervical spondylosis patients treated in our hospital from January 2013 to December 2015, the technical way of the operation of these 96 cases of loyalty if they were randomly divided into A, B, C three groups. A group of 32 cases were treated by combined anterior and posterior approach in treatment of cervical myelopathy. B group of 32 cases patients with anterior cervical corpectomy decompression and bone graft fusion internal fixation surgery to treat cervical vertebra disease, C treatment group of 32 patients used conventional posterior decompression and fusion surgery for lateral mass screw fixation. Postoperative follow-up, the three groups of X-ray intervertebral stability and fusion, neurological function JOA score and clinical efficacy of the effective date were compared. Results: Three group of patients after half a year, the JOA scores were improved, the patients of the A group after a year of score (16.3 ±1.83), the scores of B and C two groups were respectively (15.7 ±1.15),(15.59 ±1.21), there was statistically differences between the three groups (P < 0.05). After one year' s follow-up, the bone graft and internal fixation material had no loosening, displacement and subsidence, the fusion rate of A group reached 90.6%, the fusion rate of B group was 53.1%, the fusion rate of C group was 56.25%, and the difference was statistically significant (P < 0.05). For clinical effectiveness is divided into apparent, effective, fair, invalid. the effective rate of group A was 87.5%, and the effective rate was 50% in group B, the difference was statistically significant (P < 0.05). Conclusion: the treatment effect of A group was better than that of B and C two groups before and after operation, and the effect was significant. 展开更多
关键词 ANTERIOR and POSTERIOR SURGERY COMBINED therapy SPINAL cord type of cervical SPONDYLOSIS Clinical efficacy SPINAL nerve function
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大鼠脊髓神经元氧糖缺失-复氧复糖损伤时TET3诱导DNA去甲基化在甲烷上调Nrf2表达中的作用
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作者 王丽萍 郭晓明 +3 位作者 陈国忠 吴晓智 陈东生 徐皓 《中华麻醉学杂志》 CAS CSCD 北大核心 2019年第4期430-435,共6页
目的评价大鼠脊髓神经元氧糖缺失-复氧复糖损伤时10-11易位蛋白3(TET3)诱导DNA去甲基化在甲烷上调核转录因子E2相关因子2(Nrf2)表达中的作用。方法原代培养大鼠脊髓神经元,以1×105个/ml密度接种于6孔板,采用随机数字表法分为5组(n=... 目的评价大鼠脊髓神经元氧糖缺失-复氧复糖损伤时10-11易位蛋白3(TET3)诱导DNA去甲基化在甲烷上调核转录因子E2相关因子2(Nrf2)表达中的作用。方法原代培养大鼠脊髓神经元,以1×105个/ml密度接种于6孔板,采用随机数字表法分为5组(n=48):正常对照组(C组)、氧糖缺失-复氧复糖组(OGD/R组)、甲烷组(M组)、甲烷+TET3-siRNA组(M+siTET3组)和甲烷+阴性siRNA组(M+siCon组)。采用无糖-无血清Earle平衡盐液,在5%CO2-95%N2缺氧培养箱中孵育2 h ,然后正常培养的方法制备氧糖缺失-复氧复糖损伤模型。M组于复氧复糖时在培养液中加入200 μl甲烷饱和生理盐水(甲烷终浓度1.8 mmol/L);M+siTET3组和M+siCon组于复氧复糖前24 h时分别加入100 pmol/L TET3-siRNA和100 pmol/L阴性siRNA进行转染。复氧复糖12 h时,测定神经元存活率、LDH漏出率、神经元凋亡率,采用实时荧光定量PCR法检测TET3和Nrf2的mRNA表达,采用Western blot法测定核蛋白Nrf2和TET3表达水平;采用ELISA法测定神经元SOD、过氧化氢酶(CAT)和MDA含量。提取神经元DNA,采用ELISA法确定DNA甲基化率、羟甲基化率,采用实时荧光甲基化特异性PCR法检测Nrf2基因启动子CpG岛甲基化水平。结果与C组比较,OGD/R组神经元存活率降低,LDH释放率和凋亡率升高(P<0.01);与OGD/R组比较,M组神经元存活率升高,LDH释放率和凋亡率降低,TET3及其mRNA、Nrf2及其mRNA表达上调,DNA羟甲基化率、SOD和CAT含量升高,DNA及Nrf2启动子甲基化率、MDA含量降低(P<0.05或0.01);与M组比较,M+siTET3组神经元存活率降低,LDH释放率和凋亡率升高,TET3及其mRNA、Nrf2及其mRNA表达下调,DNA羟甲基化率、SOD和CAT含量降低,DNA及Nrf2启动子甲基化率、MDA含量升高(P<0.05或0.01),M+siCon组上述指标比较差异无统计学意义(P>0.05)。结论大鼠脊髓神经元氧糖缺失-复氧复糖损伤时甲烷上调Nrf2表达的机制与激活TET3,促进Nrf2启动子区DNA去甲基化有关。 展开更多
关键词 甲烷 NF-E2相关因子2 脊髓 再灌注损伤 10-11易位蛋白 DNA去甲基化
预防性手术治疗儿童无症状脊髓脂肪瘤
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作者 鲍南 杨波 +3 位作者 宋云海 秦荣 高俜娉 夏泽阳 《中华神经外科杂志》 CSCD 北大核心 2019年第8期771-775,共5页
目的探讨预防性手术治疗儿童无症状脊髓脂肪瘤的临床效果及意义。方法回顾性分析2001年4月至2015年6月上海交通大学医学院附属上海儿童医学中心神经外科采取预防性手术治疗168例无症状脊髓脂肪瘤患儿的临床资料。患儿手术年龄为1.5个月... 目的探讨预防性手术治疗儿童无症状脊髓脂肪瘤的临床效果及意义。方法回顾性分析2001年4月至2015年6月上海交通大学医学院附属上海儿童医学中心神经外科采取预防性手术治疗168例无症状脊髓脂肪瘤患儿的临床资料。患儿手术年龄为1.5个月至15.0岁(中位年龄为7个月),术前均无疼痛、大小便失禁和(或)双下肢功能障碍等神经系统症状。手术方法包括:切除皮下和椎管内脊髓外的脂肪瘤以及脊髓内的脂肪次全切除,并将脊髓包括末端圆锥从硬脊膜囊上剪开并分离,以松解脊髓拴系。结果168例患儿中,132例脊髓背侧型脂肪瘤均全切除,36例方型及包绕型脂肪瘤次全切除。5例术后出现脑脊液漏致皮下积液,经多次穿刺抽液并加压包扎后治愈。6例术后即刻出现大小便轻度失禁,其中5例1个月内完全恢复正常,1例症状持续无缓解。168例患儿中,159例获随访,随访时间为3~19年(中位随访时间为76个月)。13例(8.2%)术后远期出现症状,其中12例为脊髓粘连,1例为脊髓脂肪瘤复发。结论对于儿童无症状脊髓脂肪瘤,预防性手术治疗能够减少患儿日后神经功能障碍的发生。 展开更多
关键词 脊髓 脂肪瘤 神经外科手术 预后
The role of connexin43 in neuropathic pain induced by spinal cord injury
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作者 Anhui Wang Changshui Xu 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第6期555-561,共7页
Neuropathic pain is caused by the damage or dysfunction of the nervous system. In many neuropathic pain models, there is an increase in the number of gap junction (GJ) channels, especially the upregulation of the expr... Neuropathic pain is caused by the damage or dysfunction of the nervous system. In many neuropathic pain models, there is an increase in the number of gap junction (GJ) channels, especially the upregulation of the expression of connexin43 (Cx43), leading to the secretion of various types of cytokines and involvement in the formation of neuropathic pain. GJs are widely distributed in mammalian organs and tissues, and Cx43 is the most abundant connexin (Cx) in mammals. Astrocytes are the most abundant glial cell type in the central nervous system (CNS),which mainly express Cx43. More importantly, GJs play an important role in regulating cell metabolism, signaling, and function. Many existing literatures showed that Cx43 plays an important role in the nervous system, especially in the CNS under normal and pathological conditions. However, many internal mechanisms have not yet been thoroughly explored. In this review, we summarized the current understanding of the role and association of Cx and pannexin chan nets in neuropathic pain, especially after spinal cord injury, as well as some of our own in sights and thoughts which suggest that Cx43 may become an emerging therapeutic target for future neuropathic pain, bringing new hope to patients. 展开更多
关键词 connexin43 astrocytes gap junction NEUROPATHIC pain SPINAL CORD injury
Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats 预览
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作者 Chary M. Batista Eric D. Mariano +6 位作者 Camila S. Dale Alexandre F. Cristante Luiz R. Britto Jose P. Otoch Manoel J. Teixeira Matthias Morgalla Guilherme Lepski 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期2011-2019,共9页
Neuropathic pain after spinal cord injury(SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy;nevertheless, the ideal cell type in terms of neuroge... Neuropathic pain after spinal cord injury(SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy;nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells(fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium(sham) or fNSCs extracted from telencephalic vesicles(TV group) or the ventral medulla(VM group) of E/14 embryos. Sensory(von Frey filaments and hot plate) and motor(the Basso, Beattie,Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and VM groups at 4 and 5 weeks after transplantation, respectively. Moreover, mechanical allodynia improved in both the TV and VM groups at 8 weeks. No significant motor recovery was observed in the TV or VM groups compared with sham. Stereological analyses showed that ~70% of TV and VM cells differentiated into NeuN+ neurons,with a high proportion of enkephalinergic and GABAergic cells in the TV group and enkephalinergic and serotoninergic cells in the VM group. Our study suggests that neuronal precursors from TV and VM, once implanted into the injured spinal cord, maturate into different neuronal subtypes, mainly GABAergic, serotoninergic, and enkephalinergic, and all subtypes alleviate pain, despite no significant motor recovery. The study was approved by the Animal Ethics Committee of the Medical School of the University of S?o Paulo(protocol number 033/14) on March 4, 2016. 展开更多
关键词 spinal cord injuries chronic PAIN neural stem cells cell TRANSPLANTATION neuronal differentiation GABAERGIC NEURON serotoninergic NEURON enkephalinergic NEURON
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自身免疫性胶质纤维酸性蛋白星形细胞病患者脊髓磁共振成像及临床特征分析
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作者 徐辉明 黄清梅 +8 位作者 肖小玉 刘天妮 陈百铿 杨华才 刘思 杨洁 黄莉 龙友明 高聪 《中华神经科杂志》 CAS CSCD 北大核心 2019年第2期92-97,共6页
目的 分析国人自身免疫性胶质纤维酸性蛋白(GFAP)星形细胞病患者的脊髓病变的磁共振成像(MRI)特征及其相关临床特点。 方法 使用基于组织法及细胞法检测2013年3月至2018年6月在广州医科大学附属第二医院神经内科收集的1 040份脑脊液和... 目的 分析国人自身免疫性胶质纤维酸性蛋白(GFAP)星形细胞病患者的脊髓病变的磁共振成像(MRI)特征及其相关临床特点。 方法 使用基于组织法及细胞法检测2013年3月至2018年6月在广州医科大学附属第二医院神经内科收集的1 040份脑脊液和血清标本,发现42例GFAP抗体阳性患者,回顾性分析脑脊液GFAP抗体阳性同时存在脊髓病灶的19例自身免疫性GFAP星形细胞病患者的脊髓MRI资料及其临床资料。 结果 19例患者中,女性12例,男性7例;发病年龄(44±17)岁。患者的主要临床表现为肢体麻木无力(14/19)、视力障碍(5/19)、头痛(4/19)、癫痫发作(4/19)、认知障碍(3/19)等。脊髓MRI提示,脊髓病灶仅累及颈段脊髓者5例(5/19),仅累及胸段脊髓者8例(8/19),同时累及颈胸段脊髓者6例(6/19);脊髓受累长度超过3个脊髓节段者15例(15/19);脊髓病灶有强化者7例(7/19);脊髓病灶合并有颅内病灶者8例(8/19);病灶累及脊髓灰质19例(19/19)。 结论 自身免疫性GFAP星形细胞病患者女性多于男性,患者的脑部和脊髓可以同时受累,患者的脊髓MRI表现形式复杂,脊髓受累节段一般较长,并且以累及脊髓灰质为主,脊髓病灶多累及胸段脊髓,仅累及颈段脊髓较少,腰段脊髓基本不受累。 展开更多
关键词 自身免疫疾病 胶质纤维酸性蛋白 星形细胞 脊髓 磁共振成像
舒芬太尼对周围神经损伤小鼠脊髓神经元凋亡的影响
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作者 籍婷婷 张析哲 +6 位作者 周琪 宋健楠 曹剑 梁晓东 李海波 毕立伟 孙义 《中华麻醉学杂志》 CAS CSCD 北大核心 2019年第3期331-334,共4页
目的评价舒芬太尼对周围神经损伤小鼠脊髓神经元凋亡的影响.方法清洁级健康雄性BALB∕c小鼠150只,6~8周龄,体重18~22g,采用随机数字表法分为3组(n=50):假手术组(Sham组)、周围神经损伤组(PNI组)和舒芬太尼组(SF组).PNI组和SF组建立单侧... 目的评价舒芬太尼对周围神经损伤小鼠脊髓神经元凋亡的影响.方法清洁级健康雄性BALB∕c小鼠150只,6~8周龄,体重18~22g,采用随机数字表法分为3组(n=50):假手术组(Sham组)、周围神经损伤组(PNI组)和舒芬太尼组(SF组).PNI组和SF组建立单侧坐骨神经损伤模型,造模后SF组腹腔注射舒芬太尼5.0μg∕kg,Sham组和PNI组给予等容量生理盐水,1次∕d,连续3d.于术后1、3、7、14和28d(T0-4)时随机处死5只小鼠取脊髓L4-6节段,HE染色后光镜下观察病理学结果,采用TUNEL法检测神经元凋亡情况,计算神经元凋亡指数(AI).于T0-4时处死5只小鼠取损伤同侧脊髓L4-6节段,采用Westernblot法检测Bcl-2、Bax和活化型caspase-3的表达,计算Bcl-2表达和Bax表达的比值(Bcl-2∕Bax比值).结果与Sham组比较,PNI组和SF组AI升高,Bcl-2表达下调,活化型caspase-3和Bax表达上调(P<0.05);与PNI组比较,SF组AI降低,Bcl-2表达上调,活化型caspase-3和Bax表达下调,Bcl-2∕Bax比值升高(P<0.05).SF组比PNI组神经病理学损伤减轻.结论舒芬太尼可抑制周围神经损伤小鼠脊髓神经元凋亡. 展开更多
关键词 舒芬太尼 周围神经损伤 脊髓 细胞凋亡
Sciatic nerve injury alters the spatial arrangement of neurons and glial cells in the anterior horn of the spinal cord 预览
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作者 Ali Rashidiani-Rashidabadi Mohammad Hassan Heidari +7 位作者 Ensieh Sajadi Fatemeh Hejazi Fatemeh Fadaei Fathabady Yousef Sadeghi Abbas Aliaghaei Amir Raoofi Mohammad-Amin Abdollahifar Reza Mastery Farahni 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1833-1840,共8页
The spatial arrangement of the cell is important and considered as underlying mechanism for mathematical modeling of cell to cell interaction.The ability of cells to take on the characteristics of other cells in an or... The spatial arrangement of the cell is important and considered as underlying mechanism for mathematical modeling of cell to cell interaction.The ability of cells to take on the characteristics of other cells in an organism,it is important to understand the dynamical behavior of the cells.This method implements experimental parameters of the cell-cell interaction into the mathematical simulation of cell arrangement.The purpose of this research was to explore the three-dimensional spatial distribution of anterior horn cells in the rat spinal cord to examine differences after sciatic nerve injury.Sixteen Sprague-Dawley male rats were assigned to control and axotomy groups.Twelve weeks after surgery,the anterior horn was removed for first-and second-order stereological studies.Second-order stereological techniques were applied to estimate the pair correlation and cross-correlation functions using a dipole probe superimposed onto the spinal cord sections.The findings revealed 7% and 36% reductions in the mean volume and total number of motoneurons,respectively,and a25% increase in the neuroglial cell number in the axotomized rats compared to the control rats.In contrast,the anterior horn volume remained unchanged.The results also indicated a broader gap in the pair correlation curve for the motoneurons and neuroglial cells in the axotomized rats compared to the control rats.This finding shows a negative correlation for the distribution of motoneurons and neuroglial cells in the axotomized rats.The cross-correlation curve shows a negative correlation between the motoneurons and neuroglial cells in the axotomized rats.These findings suggest that cellular structural and functional changes after sciatic nerve injury lead to the alterations in the spatial arrangement of motoneurons and neuroglial cells,finally affecting the normal function of the central nervous system.The experimental protocol was reviewed and approved by the Animal Ethics Committee of Shahid Beheshti University of Medical Sciences(approval No.IR.SBMU.MS 展开更多
关键词 SCIATIC nerve injury spatial arrangement spinal cord MOTORNEURON neuroglial cells AXOTOMY ANTERIOR HORN
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短时程脊髓电刺激治疗亚急性期带状疱疹性神经痛的短期疗效
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作者 余倩 黄玉瑛 贺纯静 《实用疼痛学杂志》 2019年第3期182-185,共4页
目的观察短时程脊髓电刺激治疗亚急性期带状疱疹性神经痛的短期疗效。方法收集2016年1月至2018年6月贵州省人民医院疼痛科、神经内科收治的亚急性带状疱疹神经痛患者23例,年龄60~80岁,性别不限,行短时程脊髓电刺激治疗,将电极置入硬膜外... 目的观察短时程脊髓电刺激治疗亚急性期带状疱疹性神经痛的短期疗效。方法收集2016年1月至2018年6月贵州省人民医院疼痛科、神经内科收治的亚急性带状疱疹神经痛患者23例,年龄60~80岁,性别不限,行短时程脊髓电刺激治疗,将电极置入硬膜外腔,刺激脊髓后角感觉神经元及后柱传导束,治疗10~14d后拔出电极。评估治疗前后不同时点的VAS评分,采用睡眠质量评分法评估不同时点睡眠质量,并评价治疗后不同时点的疗效。结果术后1、3、7d及1、3个月时VAS评分分别为(1.48±1.27)、(1.35±1.19)、(1.13±0.97)、(2.96±1.94)、(3.34±2.14),较治疗前(6.57±1.53)明显降低,差异有统计学意义(P<0.01)。睡眠质量明显改善,术后各评价时点睡眠质量评分较术前差异有统计学意义(P<0.01);术后1、3、7d、1及3个月总体有效率分别为82.6%(19/23例),87.0%(20/23例),91.3%(21/23例),21.7%(5/23例),17.4%(4/23例)。结论短时程脊髓电刺激治疗亚急性期带状疱疹性神经痛短期效果佳,可迅速降低患者疼痛,明显改善睡眠质量。 展开更多
关键词 电刺激 脊髓 带状疱疹 神经痛 亚急性
CLIPPERS综合征一例并文献复习 预览
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作者 武翠梅 王改青 《中国脑血管病杂志》 CAS CSCD 北大核心 2019年第5期266-268,共3页
类固醇激素反应性慢性淋巴细胞性炎性反应伴脑桥血管周围强化症(CLIPPERS)综合征是一种对类固醇激素治疗敏感的慢性中枢神经系统炎性疾病,主要累及脑桥,部分患者也可同时累及多个部位。该病比较罕见,其诊断主要依据头部MR增强扫描示“... 类固醇激素反应性慢性淋巴细胞性炎性反应伴脑桥血管周围强化症(CLIPPERS)综合征是一种对类固醇激素治疗敏感的慢性中枢神经系统炎性疾病,主要累及脑桥,部分患者也可同时累及多个部位。该病比较罕见,其诊断主要依据头部MR增强扫描示“胡椒盐”样强化这一特殊的影像学改变及结合临床中其他检查手段进行诊断,需与临床表现及影像学改变与CLIPPERS综合征类似的疾病进行鉴别,给予激素治疗后可有效缓解临床症状。作者报道1例主要累及小脑和脊髓的CLIPPERS综合征,以加强对该病的认识。 展开更多
关键词 CLIPPERS综合征 小脑 脊髓
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臭氧氧化预处理减轻大鼠脊髓缺血再灌注损伤 预览
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作者 王斌 白伟 郭甲瑞 《脊柱外科杂志》 2019年第1期56-59,共4页
目的探讨臭氧氧化预处理对大鼠脊髓缺血再灌注损伤的作用及可能机制。方法将36只健康成年雄性SD大鼠随机分为假手术组、模型组和臭氧预处理组,每组12只。模型组和臭氧预处理组采用夹闭右肾动脉上腹主动脉的方法构建大鼠脊髓缺血再灌注... 目的探讨臭氧氧化预处理对大鼠脊髓缺血再灌注损伤的作用及可能机制。方法将36只健康成年雄性SD大鼠随机分为假手术组、模型组和臭氧预处理组,每组12只。模型组和臭氧预处理组采用夹闭右肾动脉上腹主动脉的方法构建大鼠脊髓缺血再灌注损伤模型,臭氧预处理大鼠于建模前7d开始以1mg/(kg·d)腹腔注射臭氧(50mg/L)-氧气混合气体至建模。分别于恢复灌流后6、12、24和48h时,对各组大鼠后肢神经运动功能进行评分。末次评分后取脊髓标本,行HE染色观察组织病理学变化,行2,3,5-氯化三苯基四氮唑(TTC)染色检测缺血面积,用试剂盒检测丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平。结果与假手术组相比,模型组和臭氧预处理组大鼠恢复灌流后6、12、24、48h神经运动功能评分均降低,差异均有统计学意义(P<0.05);与模型组相比,臭氧预处理组大鼠恢复灌流后6、12、24、48h神经运动功能评分升高,差异有统计学意义(P<0.05)。与假手术组相比,模型组和臭氧预处理组大鼠脊髓组织缺血面积增大,差异均有统计学意义(P<0.05);与模型组相比,臭氧预处理组大鼠脊髓缺血面积减小,差异有统计学意义(P<0.05)。与假手术组相比,模型组和臭氧预处理组大鼠脊髓组织中MDA水平升高,而SOD和GSH-Px水平降低,差异均有统计学意义(P<0.05);与模型组相比,臭氧预处理组大鼠脊髓组织中MDA水平降低,而SOD和GSH-Px水平升高,差异均有统计学意义(P<0.05)。结论臭氧预处理可有效减轻大鼠脊髓缺血再灌注损伤,其机制可能与抑制脊髓组织氧化应激反应有关。 展开更多
关键词 脊髓 再灌注损伤 基因表达 臭氧 氧化性应激 大鼠
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