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Recent advances in the management of gastrointestinal stromal tumor 认领
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作者 Monjur Ahmed 《世界临床病例杂志》 SCIE 2020年第15期3142-3155,共14页
Gastrointestinal stromal tumor(GIST)is a rare but an important clinical entity seen in our clinical practice.It is the most common mesenchymal tumor of the gastrointestinal tract and most common malignancy of the smal... Gastrointestinal stromal tumor(GIST)is a rare but an important clinical entity seen in our clinical practice.It is the most common mesenchymal tumor of the gastrointestinal tract and most common malignancy of the small intestine.Although the exact prevalence of GIST is not known,the incidence of GIST has been increasing.GISTs arise from interstitial cells of Cajal.Most of the GISTs occur due to mutation in c-kit gene or platelet derived growth factor receptor alpha gene.15%of GISTs do not have these mutations and they are called wildtype GISTs.Almost all GISTs express KIT receptor tyrosine kinase.Histologically,GISTs look like spindle cell tumors most of the time but they can be epitheloid or mixed type.The median size of GISTs varies from 2.7 cm to 8.9 cm.Clinically,patients with small GISTs remain asymptomatic but as the GIST size increases,patients present with various symptoms depending on the location of the GIST.Most of GISTs are located in the stomach or small bowel.Diagnosis is suspected on imaging and endoscopic studies,and confirmed by tissue acquisition with immunohistochemical staining.The aggressiveness of GISTs depends on the size,mitotic index and location.Surgical resection is the treatment of choice.But various endoscopic modalities of resection are increasingly being tried.Tyrosine kinase inhibitors are extremely useful in the management of large GISTs,unresectable GISTs and metastatic GISTs.Treatment options for metastatic GISTs also include radiotherapy,chemotherapy,hepatic artery embolization,chemoembolization and radiofrequency ablation. 展开更多
关键词 Gastrointestinal stromal tumor Mesenchymal tumor of gastrointestinal tract Gastrointestinal subepithelial tumors Management of gastrointestinal stromal tumor Familial gastrointestinal stromal tumor Risk stratification
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miR-22与肿瘤 认领
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作者 蒋如如(综述) 赵川(综述) 哈小琴(审校) 《中国生物制品学杂志》 CAS CSCD 2020年第7期832-836,共5页
越来越多的研究表明,作为miRNA其中一员的miR-22在肿瘤的发生及发展中发挥抑癌或促癌等多重作用,影响肿瘤上皮间质转化(epithelial mesenchymal transition,EMT)、增殖、迁移、侵袭、转移及凋亡等过程,在肿瘤早期诊断及指导预后方面具... 越来越多的研究表明,作为miRNA其中一员的miR-22在肿瘤的发生及发展中发挥抑癌或促癌等多重作用,影响肿瘤上皮间质转化(epithelial mesenchymal transition,EMT)、增殖、迁移、侵袭、转移及凋亡等过程,在肿瘤早期诊断及指导预后方面具有重要的指导意义。本文就miR-22与肿瘤发生、发展、早期诊断及预后的关系作一综述。 展开更多
关键词 miR-22 肿瘤 抑癌 促癌 预后
肿瘤相关中性粒细胞与肿瘤发生发展的研究进展 认领
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作者 齐潇 钟兆铭(综述) 孙传政(审校) 《昆明医科大学学报》 CAS 2020年第5期140-144,共5页
中性粒细胞是人体血液循环中最丰富的白细胞,其与抗感染及抗炎密切相关,是天然免疫系统的重要组成部分。中性粒细胞可被肿瘤细胞分泌的细胞因子招募至肿瘤微环境进而转化为肿瘤相关中性粒细胞(tumor-associated neutrophils,TANs)。TAN... 中性粒细胞是人体血液循环中最丰富的白细胞,其与抗感染及抗炎密切相关,是天然免疫系统的重要组成部分。中性粒细胞可被肿瘤细胞分泌的细胞因子招募至肿瘤微环境进而转化为肿瘤相关中性粒细胞(tumor-associated neutrophils,TANs)。TANs通过与肿瘤微环境中的肿瘤细胞和免疫细胞相互作用,在肿瘤发生发展过程中发挥重要作用。近年来越来越多的研究发现,TANs可以通过释放活性氧、分泌细胞因子及蛋白酶、释放胞外诱捕网及影响免疫细胞功能等方式影响肿瘤进展。现对近年来TANs与肿瘤发生发展关系的研究进展进行综述。 展开更多
关键词 中性粒细胞 肿瘤 肿瘤微环境
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VEGF及其靶向药物的研究进展 认领
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作者 石焕英 陈海飞 +1 位作者 李群益 施孝金 《上海医药》 CAS 2020年第15期4-7,17,共5页
肿瘤的生长转移和新血管的生成密切相关,许多调节血管生成的细胞因子参与血管的生成,而在肿瘤血管生成中起关键作用的是血管内皮细胞生长因子(vascular endothelial growth factor, VEGF)及其信号通路。阻断该通路的任何环节都会有效抑... 肿瘤的生长转移和新血管的生成密切相关,许多调节血管生成的细胞因子参与血管的生成,而在肿瘤血管生成中起关键作用的是血管内皮细胞生长因子(vascular endothelial growth factor, VEGF)及其信号通路。阻断该通路的任何环节都会有效抑制肿瘤血管的生成,进而抑制肿瘤的生长和转移。基于VEGF的调控特点,很多以VEGF及其受体VEGFR为靶点的抗肿瘤血管生成药物已经问世。本文主要概述抗VEGF的靶向药物治疗及特点。 展开更多
关键词 VEGF 肿瘤 血管生成 抗肿瘤药物 靶向治疗
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Lenvatinib for large hepatocellular carcinomas with portal trunk invasion:Two case reports 认领
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作者 Satoshi Komiyama Kazushi Numata +3 位作者 Satoshi Moriya Hiroyuki Fukuda Makoto Chuma Shin Maeda 《世界临床病例杂志》 SCIE 2020年第12期2574-2584,共11页
BACKGROUND In a phase III trial of lenvatinib as first-line treatment for advanced unresectable hepatocellular carcinoma(uHCC),the drug proved non-inferior to sorafenib in terms of the overall survival,but offered bet... BACKGROUND In a phase III trial of lenvatinib as first-line treatment for advanced unresectable hepatocellular carcinoma(uHCC),the drug proved non-inferior to sorafenib in terms of the overall survival,but offered better progression-free survival.However,the effects of lenvatinib in uHCC patients with a tumor thrombus in the main portal vein and/or a high tumor burden(tumor occupancy more than 50%of the total liver volume),remain unclear,because these were set as exclusion criteria in the aforementioned trial.CASE SUMMARY A 53-year-old man(case 1)and 66-year-old woman(case 2)with uHCC presented to us with a tumor thrombus in both the main portal vein and inferior vena cava,a high tumor burden accompanied by a tumor diameter greater than>100 mm,and distant metastasis,with the residual liver function classified as grade 2A according to the modified Albumin–Bilirubin grading.We started both patients on lenvatinib.The therapeutic effect,as evaluated by the modified Response Evaluation Criteria in Solid Tumors,was rated as partial response in both case 1 and case 2(at 8 wk and 4 wk after the start of lenvatinib administration,respectively).The therapeutic effect was sustained for 6 mo in case 1 and 20 mo in case 2.Fever occurred as an adverse event in both case 1 and 2,and hyperthyroidism and thrombocytopenia in only case 2,neither of which,however,necessitated treatment discontinuation.CONCLUSION Even in hepatocellular carcinoma patients with poor prognostic factors,if the liver function is well-preserved,lenvatinib is effective and safe. 展开更多
关键词 Hepatocellular carcinoma Lenvatinib Modified Response Evaluation Criteria in Solid Tumors Main portal vein tumor thrombus High tumor burden Case report
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胰岛素样生长因子-1在垂体瘤诊断及治疗中的应用 认领
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作者 徐兆威 柏瑞 +1 位作者 段虎斌 范益民 《中华神经创伤外科电子杂志》 2020年第3期183-186,共4页
胰岛素样生长因子-1(IGF-1)及其受体是胰岛素样生长因子系统的组成部分,在神经系统、呼吸系统及消化系统等肿瘤的发生演变过程中发挥重要作用,通过对IGF-1及其受体与肿瘤的演变关系进行干预,为肿瘤的临床诊断及治疗提供帮助。垂体瘤是... 胰岛素样生长因子-1(IGF-1)及其受体是胰岛素样生长因子系统的组成部分,在神经系统、呼吸系统及消化系统等肿瘤的发生演变过程中发挥重要作用,通过对IGF-1及其受体与肿瘤的演变关系进行干预,为肿瘤的临床诊断及治疗提供帮助。垂体瘤是颅内较常见的肿瘤,IGF-1可在垂体瘤中表达。本文从IGF-1与肿瘤的发生发展机制,及其在垂体瘤的表达与应用作一综述。 展开更多
关键词 胰岛素样生长因子-1 肿瘤 垂体瘤 肢端肥大症
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自噬调控多功能蛋白p62/SQSTM1参与肿瘤及其微环境的研究进展 认领
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作者 陈佳锋 傅修涛 丁振斌 《中国临床医学》 2020年第2期321-326,共6页
自噬是细胞内高度保守循环利用细胞代谢产物及陈旧细胞器的降解途径。自噬参与肿瘤的发生、发展及转移,甚至决定肿瘤预后。多功能蛋白p62/SQSTM1不仅作为重要的自噬接头蛋白参与降解过程,还是多个关键信号通路的调控枢纽,在肿瘤及其微... 自噬是细胞内高度保守循环利用细胞代谢产物及陈旧细胞器的降解途径。自噬参与肿瘤的发生、发展及转移,甚至决定肿瘤预后。多功能蛋白p62/SQSTM1不仅作为重要的自噬接头蛋白参与降解过程,还是多个关键信号通路的调控枢纽,在肿瘤及其微环境中发挥了重要作用。本文综述p62/SQSTM1的结构功能及其作用机制的研究进展,旨在为利用自噬调节剂治疗肿瘤寻找关键突破点提供新的理论基础。 展开更多
关键词 p62/SQSTM1 自噬 肿瘤 肿瘤微环境
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Multifaceted p21 in carcinogenesis,stemness of tumor and tumor therapy 认领
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作者 Bo-Duan Xiao Yu-Jia Zhao +3 位作者 Xiao-Yuan Jia Jiong Wu Yi-Gang Wang Fang Huang 《世界干细胞杂志:英文版(电子版)》 SCIE CAS 2020年第6期481-487,共7页
Cancer cells possess metabolic properties that are different from those of benign cells.p21,encoded by CDKN1A gene,also named p21Cip1/WAF1,was first identified as a cyclin-dependent kinase regulator that suppresses ce... Cancer cells possess metabolic properties that are different from those of benign cells.p21,encoded by CDKN1A gene,also named p21Cip1/WAF1,was first identified as a cyclin-dependent kinase regulator that suppresses cell cycle G1/S phase and retinoblastoma protein phosphorylation.CDKN1A(p21)acts as the downstream target gene of TP53(p53),and its expression is induced by wild-type p53 and it is not associated with mutant p53.p21 has been characterized as a vital regulator that involves multiple cell functions,including G1/S cell cycle progression,cell growth,DNA damage,and cell stemness.In 1994,p21 was found as a tumor suppressor in brain,lung and colon cancer by targeting p53 and was associated with tumorigenesis and metastasis.Notably,p21 plays a significant role in tumor development through p53-dependent and p53-independent pathways.In addition,expression of p21 is closely related to the resting state or terminal differentiation of cells.p21 is also associated with cancer stem cells and acts as a biomarker for such cells.In cancer therapy,given the importance of p21 in regulating the G1/S and G2 check points,it is not surprising that p21 is implicated in response to many cancer treatments and p21 promotes the effect of oncolytic virotherapy. 展开更多
关键词 P21 CDKN1A TUMORIGENESIS Circular RNA Stemness of tumor Cancer stem cells Tumor therapy
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Intratumoral heterogeneity of hepatocellular carcinoma: From singlecell to population-based studies 认领
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作者 Qi Zhang Yu Lou +1 位作者 Xue-Li Bai Ting-Bo Liang 《世界胃肠病学杂志:英文版》 SCIE CAS 2020年第26期3720-3736,共17页
Hepatocellular carcinoma(HCC)is characterized by high heterogeneity in both intratumoral and interpatient manners.While interpatient heterogeneity is related to personalized therapy,intratumoral heterogeneity(ITH)larg... Hepatocellular carcinoma(HCC)is characterized by high heterogeneity in both intratumoral and interpatient manners.While interpatient heterogeneity is related to personalized therapy,intratumoral heterogeneity(ITH)largely influences the efficacy of therapies in individuals.ITH contributes to tumor growth,metastasis,recurrence,and drug resistance and consequently limits the prognosis of patients with HCC.There is an urgent need to understand the causes,characteristics,and consequences of tumor heterogeneity in HCC for the purposes of guiding clinical practice and improving survival.Here,we summarize the studies and technologies that describe ITH in HCC to gain insight into the origin and evolutionary process of heterogeneity.In parallel,evidence is collected to delineate the dynamic relationship between ITH and the tumor ecosystem.We suggest that conducting comprehensive studies of ITH using single-cell approaches in temporal and spatial dimensions,combined with population-based clinical trials,will help to clarify the clinical implications of ITH,develop novel intervention strategies,and improve patient prognosis. 展开更多
关键词 Hepatocellular carcinoma Tumor heterogeneity Tumor microenvironment Single-cell analysis Local immunity
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Contemporary indications for and outcomes of hepatic resection for neuroendocrine liver metastases 认领
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作者 Steven D Scoville Dimitrios Xourafas +3 位作者 Aslam M Ejaz Allan Tsung Timothy Pawlik Jordan M Cloyd 《世界胃肠外科杂志:英文版(电子版)》 SCIE CAS 2020年第4期159-170,共12页
BACKGROUND Although surgical resection is associated with the best long-term outcomes for neuroendocrine liver metastases(NELM),the current indications for and outcomes of surgery for NELM from a population perspectiv... BACKGROUND Although surgical resection is associated with the best long-term outcomes for neuroendocrine liver metastases(NELM),the current indications for and outcomes of surgery for NELM from a population perspective are not well understood.AIM To determine the current indications for and outcomes of liver resection(LR)for NELM using a population-based cohort.METHODS A retrospective review of the 2014-2017 American College of Surgeons National Surgical Quality Improvement Program and targeted hepatectomy databases was performed to identify patients who underwent LR for NELM.Perioperative characteristics and 30-d morbidity and mortality were analyzed.RESULTS Among 669 patients who underwent LR for NELM,the median age was 60(interquartile range:51-67)and 51%were male.While the number of metastases resected ranged from 1 to 9,the most common(45%)number of tumors resected was one.The majority(68%)of patients had a largest tumor size of<5 cm.Most patients underwent partial hepatectomy(71%)while fewer underwent a right or left hepatectomy or trisectionectomy.The majority of operations were open(82%)versus laparoscopic(17%)or robotic(1%).In addition,30%of patients underwent intraoperative ablation while 45%had another concomitant operation including cholecystectomy(28.8%),bowel resection(20.2%),or partial pancreatectomy(3.4%).Overall 30-d morbidity and mortality was 29%and 1.3%,respectively.On multivariate analysis,American Society of Anesthesiologists class≥3[odds ratios(OR),OR=2.089,95%confidence intervals(CI):1.197-3.645],open approach(OR=1.867,95%CI:1.148-3.036),right hepatectomy(OR=1.618,95%CI:1.014-2.582),and prolonged operative time of>230 min(OR=1.731,95%CI:1.168-2.565)were associated with higher 30-d morbidity while intraoperative ablation and concomitant procedures were not.CONCLUSION LR for NELM was performed with relatively low postoperative morbidity and mortality.Concomitant procedures performed at the time of LR did not increase morbidity. 展开更多
关键词 CARCINOID NEUROENDOCRINE TUMOR Primary TUMOR RESECTION INTRAOPERATIVE ablation CHOLECYSTECTOMY Small bowel RESECTION
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免疫编辑诱导的免疫治疗耐药 认领
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作者 吕丽 林劼 《肿瘤防治研究》 CAS CSCD 2020年第4期243-250,共8页
近年来,免疫治疗在多种恶性肿瘤治疗中取得突破性进展,为肿瘤患者带来明显生存获益。然而,免疫系统在识别和杀伤肿瘤细胞的同时,出现免疫编辑诱导,导致大多数患者对免疫治疗具有先天或后天的耐药性。肿瘤免疫编辑是免疫系统抑制或促进... 近年来,免疫治疗在多种恶性肿瘤治疗中取得突破性进展,为肿瘤患者带来明显生存获益。然而,免疫系统在识别和杀伤肿瘤细胞的同时,出现免疫编辑诱导,导致大多数患者对免疫治疗具有先天或后天的耐药性。肿瘤免疫编辑是免疫系统抑制或促进肿瘤发生发展的过程,肿瘤的发生发展经历了免疫消除、免疫平衡和免疫逃逸三个阶段。在整个过程中,肿瘤的免疫原性被编辑,并获得使疾病进展的各种免疫抑制机制,致使肿瘤细胞逃避免疫系统的监测,导致肿瘤细胞免疫逃逸产生耐药。因此,揭示肿瘤免疫治疗耐药机制及克服耐药至关重要。本文主要从肿瘤免疫编辑过程背后的机制对肿瘤免疫治疗耐药作一具体阐述,为临床中克服免疫耐药取得更好疗效提供参考。 展开更多
关键词 肿瘤 免疫编辑 免疫治疗 免疫检查点 肿瘤微环境 耐药
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HMGB1在抗肿瘤免疫发生中的作用 认领
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作者 闻莲 何雪晴 +2 位作者 钱一可 陈文利 董博翰 《生命的化学》 CAS CSCD 2020年第4期483-489,共7页
高迁移率族蛋白B1(high mobility group box 1, HMGB1)是一种广泛分布于哺乳动物细胞的高度保守的核蛋白。HMGB1对肿瘤的发生发展具有一定的影响,能够通过多种信号分子途径来促进肿瘤的增殖、迁移和侵袭等。随着相关研究的不断深入,人... 高迁移率族蛋白B1(high mobility group box 1, HMGB1)是一种广泛分布于哺乳动物细胞的高度保守的核蛋白。HMGB1对肿瘤的发生发展具有一定的影响,能够通过多种信号分子途径来促进肿瘤的增殖、迁移和侵袭等。随着相关研究的不断深入,人们发现,HMGB1与免疫细胞抗肿瘤作用的发挥也有密切联系。因此,HMGB1有望成为肿瘤免疫治疗的重要靶点之一。然而,HMGB1生物学功能复杂,对肿瘤免疫的影响作用也不单一。为了更好地将HMGB1应用于肿瘤治疗,本文就HMGB1在抗肿瘤免疫发生中的作用及其机制的研究进展作一综述。 展开更多
关键词 高迁移率族蛋白B1 肿瘤 免疫细胞 抗肿瘤免疫
Soft Mesoporous Organosilica Nanoplatforms Improve Blood Circulation,Tumor Accumulation/Penetration,and Photodynamic Efficacy 认领
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作者 Xin Peng Kun Chen +7 位作者 Wanhua Liu Xiongfeng Cao Mengru Wang Jun Tao Ying Tian Lei Bao Guangming Lu Zhaogang Teng 《纳微快报:英文版》 SCIE EI CAS CSCD 2020年第10期205-223,共19页
To date,the ability of nanoplatforms to achieve excellent therapeutic responses is hindered by short blood circulation and limited tumor accumulation/penetration.Herein,a soft mesoporous organosilica nanoplatform modi... To date,the ability of nanoplatforms to achieve excellent therapeutic responses is hindered by short blood circulation and limited tumor accumulation/penetration.Herein,a soft mesoporous organosilica nanoplatform modified with hyaluronic acid and cyanine 5.5 are prepared,denoted SMONs-HA-Cy5.5,and comparative studies between SMONs-HA-Cy5.5(24.2 MPa)and stiff counterparts(79.2 MPa)are conducted.Results indicate that,apart from exhibiting a twofold increase in tumor cellular uptake,the soft nanoplatforms also display a remarkable pharmacokinetic advantage,resulting in considerably improved tumor accumulation.Moreover,SMONs-HA-Cy5.5 exhibits a significantly higher tumor penetration,achieving 30-μm deeper tissue permeability in multicellular spheroids relative to the stiff counterparts.Results further reveal that the soft nanoplatforms have an easier extravasation from the tumor vessels,diffuse farther in the dense extracellular matrix,and reach deeper tumor tissues compared to the stiff ones.Specifically,the soft nanoplatforms generate a 16-fold improvement(43 vs.2.72μm)in diffusion distance in tumor parenchyma.Based on the significantly improved blood circulation and tumor accumulation/penetration,a soft therapeutic nanoplatform is constructed by loading photosensitizer chlorin e6 in SMONs-HA-Cy5.5.The resulting nanoplatform exhibits considerably higher therapeutic efficacy on tumors compared to the stiff ones. 展开更多
关键词 Mesoporous organosilica Soft nanoplatform Long circulation Tumor accumulation Tumor penetration
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核糖体生物合成与肿瘤的研究进展 认领
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作者 钱丽丽 张红河 《肿瘤防治研究》 CAS CSCD 2020年第5期393-397,共5页
核糖体生物合成是细胞内重要的生物学过程,决定了细胞内蛋白质合成的速率。在肿瘤细胞旺盛的生长压力下,肿瘤中核糖体生物合成发生异常调控。有越来越多的研究表明核糖体生物合成的异常可能会促进肿瘤的发生发展,并且它也逐渐被认为是... 核糖体生物合成是细胞内重要的生物学过程,决定了细胞内蛋白质合成的速率。在肿瘤细胞旺盛的生长压力下,肿瘤中核糖体生物合成发生异常调控。有越来越多的研究表明核糖体生物合成的异常可能会促进肿瘤的发生发展,并且它也逐渐被认为是多种肿瘤的一个可行的治疗靶点。本文简要综述了核糖体生物合成过程及其在肿瘤中的异常和调控,以及药物靶向核糖体生物合成治疗肿瘤的研究进展。 展开更多
关键词 核糖体生物合成 肿瘤 肿瘤治疗
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miR-605-3p通过靶向ZIK1抑制肝细胞癌迁移和侵袭 认领
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作者 胡艳 黄智平 +2 位作者 周维 张红卫 俞雷 《实用肿瘤杂志》 CAS 2020年第1期30-36,共7页
目的探讨miR-605-3p对肝细胞癌细胞迁移和侵袭能力的影响及其相关机制。方法采用定量PCR检测80例肝癌组织及其配对癌旁组织以及肝癌细胞株(Huh7、HCC-LM3、Hep3B以及SMMC-7721)和肝细胞株(THLE-3)中miR-605-3p的表达水平。生物信息学分... 目的探讨miR-605-3p对肝细胞癌细胞迁移和侵袭能力的影响及其相关机制。方法采用定量PCR检测80例肝癌组织及其配对癌旁组织以及肝癌细胞株(Huh7、HCC-LM3、Hep3B以及SMMC-7721)和肝细胞株(THLE-3)中miR-605-3p的表达水平。生物信息学分析预测miR-605-3p结合的靶蛋白,Western blot检验ZIK1蛋白的表达情况,荧光素酶报告验证miR-605-3p与ZIK1之间的相互关联。Kaplan-Meier曲线和Log-rank分析方法比较miR-605-3p高、低表达组无进展生存期和总生存期。将SMMC-772细胞分为模拟物转染组、抑制物转染组、模拟物对照组和抑制物对照组。CCK8增殖实验检测细胞增殖能力,细胞划痕实验和transwell侵袭实验检测细胞迁移和侵袭。结果miR-605-3p在肝癌组织中表达下调(P<0.05)。以miR-605-3p在肝癌组织中位表达分为高表达和低表达组,miR-605-3p在肝癌组织低表达患者5年总生存率(21.6%vs 48.3%,P<0.05)和无进展生存率均低于高表达患者(9.5%vs 30.8%,P<0.05)。miR-605-3p抑制剂可以增强肝癌细胞迁移和侵袭能力(均P<0.05)。生物信息学分析证实,ZIK1蛋白是miR-605-3p肝癌细胞中的直接作用靶点。结论miR-605-3p在肝癌发生和发展过程中发挥作用,通过调控ZIK1的表达影响肝癌细胞的迁移和侵袭能力。 展开更多
关键词 肝细胞/病理学 微RNAS 细胞运动 肿瘤侵润 生物标记 肿瘤 肿瘤细胞 培养的 聚合酶链反应
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Experimental study on the relationship between traumatic stress and tumor growth,proliferation,and metastasis 认领
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作者 Weigang Cao Baoan Qiu 《肿瘤学与转化医学:英文版》 2020年第2期52-56,共5页
Objective This study aimed to investigate the relationship between traumatic stress and tumor growth,proliferation,and metastasis.Methods A scalding method was used as an injurious factor to induce traumatic stress in... Objective This study aimed to investigate the relationship between traumatic stress and tumor growth,proliferation,and metastasis.Methods A scalding method was used as an injurious factor to induce traumatic stress in Wistar rats.The rats were randomly divided into three groups—the control group,mild-scald group,and severe-scald group,with 14 rats in each group.Wistar rats were used to subculture the Walker-256 cell line for the generation of tumor ascites.Tumor cells from the ascites were cultured and used to establish a rat subcutaneous xenograft model.After 7 days,the mild-burn group and the severe-burn group were subjected burns to 10%and 15%of their backs,respectively.Blood was taken from the tail vein of rats at different times to detect changes in blood cortisol,IL-1β,and TNF-αlevels.Pathological specimens were collected 14 days later,and immunohistochemistry was performed to examine vascular endothelial growth factor(VEGF),proliferating cell nuclear antigen(PCNA),E-cadherin,and vimentin.Results Cortisol,IL-1βand TNF-αlevels were significantly higher in the scalding groups than in the control group.Tumor examination was performed after 14 days.The changes in tumor size showed that the tumor volume in the control group(0.593±0.195 cm3)and the mild-scald group(0.782±0.344 cm3)were not significantly different.However,the tumor volume was significantly larger in the severeburn group(1.806±0.838 cm3)than in the control and the mild-burn groups(P<0.05).Tumor tissue immunohistochemistry showed that the percentage of cells expressing PCNA in the control group,mildscald group,and severe-scald group was 57.1%,71.4%and 85.7%,respectively,and the differences among the groups were statistically significant.The number of VEGF-positive cells in the mild-and severescald groups was significantly higher than those of the control group(P<0.05).The number of E-cadherinpositive cells in the tumor tissues was significantly lower in the severe-scald group than that in the control and mild-scald groups.Vimentin showed th 展开更多
关键词 stress response Wistar rats TUMOR SCALDING
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体外三维培养技术在肿瘤中的应用及进展 认领
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作者 孙跃峰 江剑 +1 位作者 王玲 王宏 《大连医科大学学报》 CAS 2020年第2期166-171,共6页
肿瘤是一种由多种致癌基因功能的获得,抑癌基因功能的丢失以及环境因素共同决定的复杂疾病。肿瘤细胞的表型异质性、细胞生物学背景以及肿瘤微环境是决定肿瘤发展过程的关键因素。体外三维细胞培养(three-dimensional cell culture,3DCC... 肿瘤是一种由多种致癌基因功能的获得,抑癌基因功能的丢失以及环境因素共同决定的复杂疾病。肿瘤细胞的表型异质性、细胞生物学背景以及肿瘤微环境是决定肿瘤发展过程的关键因素。体外三维细胞培养(three-dimensional cell culture,3DCC)可以更好地模拟真实的肿瘤细胞微环境,对探索肿瘤的发生、侵袭以及转移的分子机制起到了决定性作用。本文将结合近年来相关文献,对3DCC的建立方法及其在恶性肿瘤中的应用进展作一综述。 展开更多
关键词 三维细胞培养 肿瘤 支架 肿瘤微环境
肿瘤微环境调控能量代谢的机制及分子影像在该领域的研究进展 认领
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作者 张国建 白智刚 +2 位作者 王文睿 王雪梅 鲁海文 《医学综述》 2020年第1期59-62,70共5页
目前肺癌的各种治疗方法大多集中于肿瘤细胞本身,对于肿瘤生存的微环境及肿瘤能量代谢关注较少。肿瘤能量代谢对肺癌的发生、发展、侵袭及转移具有极其重要的作用,而乏氧微环境可能是驱动能量代谢的关键因素,但作用机制尚未完全阐明。... 目前肺癌的各种治疗方法大多集中于肿瘤细胞本身,对于肿瘤生存的微环境及肿瘤能量代谢关注较少。肿瘤能量代谢对肺癌的发生、发展、侵袭及转移具有极其重要的作用,而乏氧微环境可能是驱动能量代谢的关键因素,但作用机制尚未完全阐明。针对肿瘤微环境与能量代谢的关系及机制进行探讨,能够指导临床从能量代谢的角度寻求肿瘤治疗策略,为肿瘤治疗提供有效靶点。而利用正电子发射计算机断层显像/CT、磁共振成像等分子影像学检查方法对肿瘤能量代谢进行活体水平评估,能为临床提供一种无创的检测手段。 展开更多
关键词 肿瘤 分子影像 肿瘤微环境 能量代谢
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Torsion of Ovarian Tumor in the Elderly: About a Case 认领
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作者 Amadou Bah Ibrahima Diakite +18 位作者 Amadou Maiga Boubacar Yoro Sidibe Madiassa Konaté Zakari Saye Boureima Kelly Tany Koné Siaka Konate Arouna A. Doumbia Bathio Traore Boubacar Karembe Mohamed Lamine Diakite Amadou A. Traoré Abdoulaye Diarra Moustapha Issa Mangane Abdoul Hamidou Almeimoune Bakary T. Dembélé Alhassane Traoré Lassana Kanté Adégné Pierre Togo 《外科学(英文)》 2020年第4期69-73,共5页
We report a case of torsion of an ovarian tumor in a 68-year-old woman with no medical and surgical history. The diagnosis was made in front of an intermittently painful pelvic mass. The treatment consisted of a left ... We report a case of torsion of an ovarian tumor in a 68-year-old woman with no medical and surgical history. The diagnosis was made in front of an intermittently painful pelvic mass. The treatment consisted of a left annexectomy;the anatomopathological examination revealed a fibro-inflammatory and hemorrhagic cyst. Postoperative results were simple with a 12-month follow-up. 展开更多
关键词 TUMOR OVARY CYST SURGERY
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Clinical and pathological characteristics and prognosis of 132 cases of rectal neuroendocrine tumors 认领
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作者 Yong-Jun Yu Yu-Wei Li +3 位作者 Yang Shi Zhao Zhang Min-Ying Zheng Shi-Wu Zhang 《世界胃肠肿瘤学杂志:英文版(电子版)》 SCIE CAS 2020年第8期893-902,共10页
BACKGROUND Neuroendocrine tumors(NETs)frequently occur in the gastrointestinal tract,lung,and pancreas,and the rectum and appendix are the sites with the highest incidence.Epidemiology statistics show that an estimate... BACKGROUND Neuroendocrine tumors(NETs)frequently occur in the gastrointestinal tract,lung,and pancreas,and the rectum and appendix are the sites with the highest incidence.Epidemiology statistics show that an estimated 8000 people every year in the United States are diagnosed with NETs occurring in the gastrointestinal tract,including the stomach,intestine,appendix,colon,and rectum.The pathological changes and clinical symptoms of NETs are not specific,and therefore they are frequently misdiagnosed.AIM To investigate the clinical symptoms,pathological characteristics,treatment,and prognosis of rectal neuroendocrine tumors(RNETs)by analyzing the clinical and pathological data of 132 RNET cases at our hospital.METHODS All RNETs were graded according to Ki-67 positivity and mitotic events.The tumors were staged as clinical stages I,II,III,and IV according to infiltrative depth and tumor size.COX proportional hazard model was used to assess the main risk factors for survival.RESULTS These 132 RNETs included 83 cases of G1,21 cases of G2,and 28 cases of G3(neuroendocrine carcinoma)disease.Immunohistochemical staining showed that 89.4%of RNETs were positive for synaptophysin and 39.4%positive for chromogranin A.There were 19,85,23,and 5 cases of clinical stages I,II,III,and IV,respectively.The median patient age was 52.96 years.The diameter of tumor,depth of invasion,and pathological grade were the main reference factors for the treatment of RNETs.The survival rates at 6,12,36,and 60 mo after operation were 98.5%,94.6%,90.2%,and 85.6%,respectively.Gender,tumor size,tumor grade,lymph node or distant organ metastasis,and radical resection were the main factors associated with prognosis of RNETs.Multivariate analysis showed that tumor size and grade were independent prognostic factors.CONCLUSION The clinical symptoms of RNETs are not specific,and they are easy to misdiagnose.Surgery is the main treatment method.The grade and stage of RNETs are the main indices to evaluate prognosis. 展开更多
关键词 Neuroendocrine tumors PROGNOSIS Univariate analysis Tumor size Tumor grade Neuroendocrine carcinoma
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