The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35 °C. The endoglucana...The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35 °C. The endoglucanase activity, melt point temperature was 1.13 folds(247.02 U·ml-1), 2.1 °C higher(39.2 °C) in 6% ethanol solution than that(218.60 U·ml-1),(37.1 °C) in free ethanol. At 40 °C–55 °C, Gibbs free energy, ΔG, and the content ofα-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.展开更多
<正>Nowadays,the structural differentiation of Chinese economy is getting clearer.In order to adapt to the change,there is an urgent need to improve supply-side environment through supply-side reform,optimize su...<正>Nowadays,the structural differentiation of Chinese economy is getting clearer.In order to adapt to the change,there is an urgent need to improve supply-side environment through supply-side reform,optimize supply-side mechanism,motivate microeconomic units and add new driving forces to sustain a long and steady ecnomic development in China.Therefore,"supply side reform"and the展开更多
基金Science and Technology Research Project of Hubei Provicial Department of Education(B2017041).
文摘The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35 °C. The endoglucanase activity, melt point temperature was 1.13 folds(247.02 U·ml-1), 2.1 °C higher(39.2 °C) in 6% ethanol solution than that(218.60 U·ml-1),(37.1 °C) in free ethanol. At 40 °C–55 °C, Gibbs free energy, ΔG, and the content ofα-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.
文摘<正>Nowadays,the structural differentiation of Chinese economy is getting clearer.In order to adapt to the change,there is an urgent need to improve supply-side environment through supply-side reform,optimize supply-side mechanism,motivate microeconomic units and add new driving forces to sustain a long and steady ecnomic development in China.Therefore,"supply side reform"and the