Dental pulp stem cells are a type of adult stem cells with strong proliferative ability and multi-differentiation potential. There are no studies on treatment of vascular dementia with dental pulp stem cells. In the p...Dental pulp stem cells are a type of adult stem cells with strong proliferative ability and multi-differentiation potential. There are no studies on treatment of vascular dementia with dental pulp stem cells. In the present study, rat models of vascular dementia were established by two-vessel occlusion, and 30 days later, rats were injected with 2 × 10~7 dental pulp stem cells via the tail vein. At 70 days after vascular dementia induction, dental pulp stem cells had migrated to the brain tissue of rat vascular dementia models and differentiated into neuronlike cells. At the same time, doublecortin, neurofilament 200, and Neu N m RNA and protein expression levels in the brain tissue were increased, and glial fibrillary acidic protein m RNA and protein expression levels were decreased. Behavioral testing also revealed that dental pulp stem cell transplantation improved the cognitive function of rat vascular dementia models. These findings suggest that dental pulp stem cell transplantation is effective in treating vascular dementia possibly through a paracrine mechanism. The study was approved by the Animal Ethics Committee of Harbin Medical University(approval No. KY2017-132) in 2017.展开更多
背景:股骨头坏死是骨伤科常见的疑难病症,致残率高,目前对于其发病机制仍然没有一个明确的定论,也未找到彻底治愈该疾病的理想方法。因此,需要借助动物模型来进一步研究该疾病的病因、发病机制、治疗方法等。目的:综述近年来常用的一些...背景:股骨头坏死是骨伤科常见的疑难病症,致残率高,目前对于其发病机制仍然没有一个明确的定论,也未找到彻底治愈该疾病的理想方法。因此,需要借助动物模型来进一步研究该疾病的病因、发病机制、治疗方法等。目的:综述近年来常用的一些股骨头坏死动物模型,总结分析出每一类动物模型对应的临床症状和模型的优缺点,从而为后续研究提供基础。方法:以"股骨头坏死,动物模型,femoral head necrosis,animal model"等为检索关键词,在万方、维普、中国知网、PubMed、Embase、Medline等数据库中检索2000年1月至2019年12月的文献,根据标准最终纳入42篇参考文献。结果与结论:①股骨头坏死动物模型已经用于相关研究多年,这确实提高了对此类骨科疾病的理解和研究;②但是目前股骨头坏死的相关研究仍然需要进一步深入探索,通过建立更理想、更完善的动物模型可在该研究领域实现新的突破。展开更多
基金supported by Yu Weihan Fund for Distinguished Young Scholars of Harbin Medical University of China,No. 002000013 (to XMZ)。
文摘Dental pulp stem cells are a type of adult stem cells with strong proliferative ability and multi-differentiation potential. There are no studies on treatment of vascular dementia with dental pulp stem cells. In the present study, rat models of vascular dementia were established by two-vessel occlusion, and 30 days later, rats were injected with 2 × 10~7 dental pulp stem cells via the tail vein. At 70 days after vascular dementia induction, dental pulp stem cells had migrated to the brain tissue of rat vascular dementia models and differentiated into neuronlike cells. At the same time, doublecortin, neurofilament 200, and Neu N m RNA and protein expression levels in the brain tissue were increased, and glial fibrillary acidic protein m RNA and protein expression levels were decreased. Behavioral testing also revealed that dental pulp stem cell transplantation improved the cognitive function of rat vascular dementia models. These findings suggest that dental pulp stem cell transplantation is effective in treating vascular dementia possibly through a paracrine mechanism. The study was approved by the Animal Ethics Committee of Harbin Medical University(approval No. KY2017-132) in 2017.
文摘背景:股骨头坏死是骨伤科常见的疑难病症,致残率高,目前对于其发病机制仍然没有一个明确的定论,也未找到彻底治愈该疾病的理想方法。因此,需要借助动物模型来进一步研究该疾病的病因、发病机制、治疗方法等。目的:综述近年来常用的一些股骨头坏死动物模型,总结分析出每一类动物模型对应的临床症状和模型的优缺点,从而为后续研究提供基础。方法:以"股骨头坏死,动物模型,femoral head necrosis,animal model"等为检索关键词,在万方、维普、中国知网、PubMed、Embase、Medline等数据库中检索2000年1月至2019年12月的文献,根据标准最终纳入42篇参考文献。结果与结论:①股骨头坏死动物模型已经用于相关研究多年,这确实提高了对此类骨科疾病的理解和研究;②但是目前股骨头坏死的相关研究仍然需要进一步深入探索,通过建立更理想、更完善的动物模型可在该研究领域实现新的突破。