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过夜培养对不同质量冻融胚胎治疗结局的影响 预览
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作者 吴睿 张丽蓉 +3 位作者 胡志富 于成和 张若鹏 罗大极 《大理大学学报》 CAS 2019年第4期68-70,共3页
目的:探讨过夜培养对不同质量冻融胚胎、囊胚治疗结局的影响。方法:回顾性分析大理大学第一附属医院开展的1 326个冻融胚胎移植(FET)周期的患者资料。根据实验目的,分为过夜培养组和常规培养组,对比分析两组间优质胚胎、非优质胚胎、优... 目的:探讨过夜培养对不同质量冻融胚胎、囊胚治疗结局的影响。方法:回顾性分析大理大学第一附属医院开展的1 326个冻融胚胎移植(FET)周期的患者资料。根据实验目的,分为过夜培养组和常规培养组,对比分析两组间优质胚胎、非优质胚胎、优质囊胚、非优质囊胚移植患者的临床妊娠率。结果:优质胚胎、非优质囊胚移植患者的临床妊娠率在两组间差异无统计学意义(P>0.05)。过夜培养组非优质胚胎移植患者的临床妊娠率显著高于常规培养组(36.36%vs. 25.89%,P=0.021),而过夜培养组优质囊胚移植患者的临床妊娠率显著低于常规培养组(51.25%vs. 65.13%,P=0.040)。结论:过夜培养有利于改善非优质冻融胚胎的治疗结局,而不利于优质冻融囊胚的治疗。 展开更多
关键词 胚胎 过夜培养 胚胎质量 冻融胚胎
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The role of p53 in developmental syndromes
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作者 Margot E.Bowen Laura D.Attardi 《分子细胞生物学报:英文版》 SCIE CAS CSCD 2019年第3期200-211,共12页
While it is well appreciated that loss of the p53 tumor suppressor protein promotes cancer,growing evidence indicates that increased p53 activity underlies the developmental defects in a wide range of genetic syndrome... While it is well appreciated that loss of the p53 tumor suppressor protein promotes cancer,growing evidence indicates that increased p53 activity underlies the developmental defects in a wide range of genetic syndromes.The inherited or de novo mutations that cause these syndromes affect diverse cellular processes,such as ribosome biogenesis,DNA repair,and centriole duplication,and analysis of human patient samples and mouse models demonstrates that disrupting these cellular processes can activate the p53 pathway.Importantly,many of the developmental defects in mouse models of these syndromes can be rescued by loss of p53,indicating that inappropriate p53 activation directly contributes to their pathogenesis.A role for p53 in driving developmental defects is further supported by the observation that mouse strains with broad p53 hyperactivation,due to mutations affecting p53 pathway components,display a host of tissue-specific developmental defects,including hematopoietic,neuronal,craniofacial,cardiovascular,and pigmentation defects.Furthermore,germline activating mutations in TP53 were recently identified in two human patients exhibiting bone marrow failure and other developmental defects.Studies in mice suggest that p53 drives developmental defects by inducing apoptosis,restraining proliferation,or modulating other developmental programs in a cell type-dependent manner.Here,we review the growing body of evidence from mouse models that implicates p53 as a driver of tissue-specific developmental defects in diverse genetic syndromes. 展开更多
关键词 P53 Mdm2 development EMBRYO CONGENITAL defect syndrome genetic DISORDER
培养程序及培养基成分对茄子优选植株花药培养的影响 预览
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作者 王畅 崔群香 +4 位作者 朱海佳 周祯 陈丹阳 汪辉 刘方娇 《蔬菜》 2019年第4期15-21,共7页
为提高茄子单倍体育种的效率,以优选植株的花蕾为试材,研究不同成分的培养基及培养程序对茄子花药培养胚状体发生和发育的影响。结果表明:诱导培养基中糖的种类和浓度以及聚乙二醇(PEG)浓度,分化、成熟、生根培养基中生长调节剂的浓度... 为提高茄子单倍体育种的效率,以优选植株的花蕾为试材,研究不同成分的培养基及培养程序对茄子花药培养胚状体发生和发育的影响。结果表明:诱导培养基中糖的种类和浓度以及聚乙二醇(PEG)浓度,分化、成熟、生根培养基中生长调节剂的浓度和种类等都会影响胚状体的发生和发育。诱导培养基使用蔗糖时,胚状体产生早、发育较成熟但数量少;使用葡萄糖,则胚状体产生数量多,但发育慢;分化培养基中细胞分裂素浓度过高、培养时间过长均不利于形成正常形态的胚状体,导致成苗困难;最终形成了培养结果稳定、可多次重复的茄子花药培养程序:花药→诱导培养基S1培养15 d(36黑暗条件下培养6 d,25光照16 h黑暗8 h培养9 d)→分化培养基B2培养20 d→胚成熟培养基培养20 d→出胚花药生根培养基培养20 d→萌发胚胎生根培养基成苗。 展开更多
关键词 茄子 花药培养 胚状体 植株再生
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促性腺激素释放激素拮抗剂在卵巢过度刺激综合征治疗中应用价值研究 预览
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作者 陈咏 于月新 《临床军医杂志》 CAS 2019年第2期170-172,共3页
目的探讨促性腺激素释放激素拮抗剂在卵巢过度刺激综合征治疗中的应用价值。方法选取北部战区总医院生殖医学中心2016年1-4月收治的36例卵巢过度刺激综合征患者为研究对象。按照随机数字表法将患者分入两组,每组18例。A组患者接受常规治... 目的探讨促性腺激素释放激素拮抗剂在卵巢过度刺激综合征治疗中的应用价值。方法选取北部战区总医院生殖医学中心2016年1-4月收治的36例卵巢过度刺激综合征患者为研究对象。按照随机数字表法将患者分入两组,每组18例。A组患者接受常规治疗,B组患者在常规治疗基础上加用促性腺激素释放激素拮抗剂。比较两组患者促黄体激素、雌二醇及获卵数、受精数、优质胚胎数。结果两组患者均未出现严重并发症。B组促黄体激素为(2.6±1.3)的U/L,明显低于A组的(4.1±1.5)U/L,差异有统计学意义(P<0.05)。B组雌二醇为(986.4±648.5)U/L,明显低于A组的(1623.5±632.6)U/L,差异有统计学意义(P<0.05)。B组获卵数、受精数、优质胚胎数分别为(36.5±4.1)个、(22.8±5.2)个、(9.5±2.2)个,均多于A组的(22.1±4.3)个、(13.4±5.3)个、(6.4±2.3)个,差异有统计学意义(P<0.05)。结论促性腺激素释放激素拮抗剂治疗卵巢过度刺激综合征安全有效,可控制疾病进展,也可用于疾病预防。 展开更多
关键词 促性腺激素释放激素拮抗剂 卵巢过度刺激综合征 促黄体激素 雌二醇 胚胎
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犏牛高效生产新模式的建立
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作者 熊显荣 李键 +5 位作者 字向东 兰道亮 何世明 吴锦波 余忠华 王二耀 《中国兽医学报》 CAS CSCD 北大核心 2019年第5期1007-1013,共7页
犏牛是牦牛与奶(肉)牛杂交的后代,具有明显的杂种优势,是牦牛育种改良的重要方向之一。由于雄性犏牛不育,杂种优势无法稳定遗传。因此,利用现代繁殖育种技术是解决此问题的关键。本研究利用牦牛屠宰场的卵巢收集卵母细胞,与奶(肉)牛的... 犏牛是牦牛与奶(肉)牛杂交的后代,具有明显的杂种优势,是牦牛育种改良的重要方向之一。由于雄性犏牛不育,杂种优势无法稳定遗传。因此,利用现代繁殖育种技术是解决此问题的关键。本研究利用牦牛屠宰场的卵巢收集卵母细胞,与奶(肉)牛的冻精进行体外受精,将获得的胚胎移植到同期发情的犏牛体内,建立生产犏牛新模式。结果表明,用荷斯坦牛、西门塔尔牛和娟姗牛的冻精与牦牛卵母细胞体外受精,受精率无显著差异(P>0.05),但显著高于牦牛种内体外受精(P<0.05)。胚胎发育动力学结果表明,犏牛胚胎的体外发育速率显著高于牦牛胚胎(P<0.05);雄性犏牛胚胎的发育显著快于雌性胚胎。用孕酮阴道栓、马绒毛膜促性腺激素(eCG)和氯前列腺烯醇(PG)组合的同期发情处理方案,诱导犏牛发情效果较好,发情率显著高于单独使用PG组(P<0.05)。获得了全球首批试管犏牛(7头),实现了犏牛生犏牛新模式。获得的试管犏牛体型丰满、生长迅速、1周岁的体质量达160 kg,是同龄牦牛的2倍。综上,本研究建立了一套高效的犏牛体外生产技术体系,充分利用了母犏牛的繁殖性能,为挖掘优秀母犏牛的繁殖潜能和加速扩繁犏牛奠定了基础,对实现少数民族地区牧民增收与生态保护具有重要的意义。 展开更多
关键词 犏牛 体外生产 胚胎 繁殖
天葵胚和胚乳的发育研究 预览
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作者 徐晶晶 张依楠 廖海民 《山地农业生物学报》 2019年第1期84-87,共4页
利用石蜡切片技术,对天葵胚和胚乳的发育进行了研究。结果表明:胚的发育属于柳叶菜型,其发育过程包括原胚、心形胚、鱼雷形胚和成熟胚时期,成熟胚的胚柄退化,胚乳发育为核型胚乳类型,游离核时期较短,成熟种子中仅剩1到2层胚乳细胞。
关键词 天葵 发育 核型胚乳
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辣椒自然游离小孢子胚状体诱导研究 预览
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作者 高素燕 吕敬刚 +3 位作者 焦荻 商纪鹏 焦定量 黄亚杰 《天津农业科学》 CAS 2019年第4期11-14,共4页
为建立辣椒自然游离小孢子培养技术体系,以32个辣椒品种为试材,研究基因型(CL-1~CL-32)、基本培养基(MS、NitschandNitsch、NLN、B5)、激素配比(ZT0.5mg·L^-1+IAA0.8mg·L^-1、ZT0.5mg·L^-1+IAA1.0mg·L^-1、ZT1.0mg&... 为建立辣椒自然游离小孢子培养技术体系,以32个辣椒品种为试材,研究基因型(CL-1~CL-32)、基本培养基(MS、NitschandNitsch、NLN、B5)、激素配比(ZT0.5mg·L^-1+IAA0.8mg·L^-1、ZT0.5mg·L^-1+IAA1.0mg·L^-1、ZT1.0mg·L^-1+IAA1.0mg·L^-1、ZT1.5mg·L^-1+IAA1.5mg·L^-1)、活性炭添加量(0.1,0.5,0.8,1.0g·L^-1)对辣椒小孢子胚状体诱导的影响。结果表明,基因型是辣椒小孢子胚状体诱导的关键因素,供试的32个基因型中有18个诱导成功,诱导成功率56.25%,其中F1代诱导成功率达到66.67%,而自交系诱导成功率为0,各基因型中以CL-14诱导率最高,达到29.2%;不同基因型最适培养基不同,5个基因型(CL-4,CL-8,CL-10,CL-12,CL-14)中CL-4、CL-12、CL-14均以NitschandNitsch为基本培养基添加适量激素诱导胚状体最佳;不同基因型的适宜激素浓度配比不同,3个基因型(CL-4、CL-8、CL-14)中CL-8、CL-14以添加0.5mg·L^-1ZT和1.0mg·L^-1 IAA效果最好;适量添加活性炭可提高小孢子胚诱导率,CL-4、CL-8、CL-14分别以添加0.8,0.5,0.5g·L^-1诱导效果最好。 展开更多
关键词 辣椒 小孢子培养 花药培养 胚状体 单倍体
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Factors affecting early embryonic development in cattle:relevance for bovine cloning
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作者 Yanna DANG Kun ZHANG 《农业科学与工程前沿:英文版》 2019年第1期33-41,共9页
Female infertility represents a major challenge for improving the production ef?ciency in the dairy industry. Historically, fertility has declined whereas milk yield has increased tremendously due to intensive genetic... Female infertility represents a major challenge for improving the production ef?ciency in the dairy industry. Historically, fertility has declined whereas milk yield has increased tremendously due to intensive genetic selection. In vivo evidence reveals about 60% pregnancy loss takes place during the ?rst month following fertilization. Meanwhile, early embryo development is signi?cant for somatic cell nuclear transfer in cattle as a large proportion of cloned embryos fail to develop beyond periimplantation stage. Oocyte quality is of utmost importance for the early embryo to develop to term for both fertilized and cloned embryos. Epigenetic reprogramming is a key process occurring after fertilization and critical roles of epigenetic modi?ers during preimplantation development are now clear. Incomplete epigenetic reprogramming is believed to be a major limitation to cloning ef?ciency.Treatment of cloned embryos with epigenetic modifying drugs(e.g., Trichostatin A) could greatly improve cloning ef?ciency in both mice and cattle. Recently, the rapid progress in high-throughput sequencing technologies has enabled detailed deciphering of the molecular mechanisms underlying these events. The robust ef?ciency of genomic editing tools also presents an alternative approach to the functional annotation of genes critical to early development. 展开更多
关键词 BOVINE CLONING EMBRYO development SOMATIC cell nuclear transfer X-inactive speci?c TRANSCRIPT
Cryopreservation of farm animal gametes and embryos:recent updates and progress
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作者 Zhengyuan HUANG Lei GAO +2 位作者 Yunpeng HOU Shien ZHU Xiangwei FU 《农业科学与工程前沿:英文版》 2019年第1期42-53,共12页
Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particu... Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously re?ned over the years to achieve greater reproductive performance;cryoprotective agents are more effective and less toxic than previously;there has been signi?cant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen–thawed porcine sperm in pig production. 展开更多
关键词 vitri?cation GAMETES EMBRYO ANIMAL production cryoprotective agent freezability
Extracts from Paeonia lactiflora Pallas, Rehmannia Glutinosa var. Purpurea Makino, Perilla Frutescens var. Acuta Kudo may increase the endometrial receptivity through expression of leukemia inhibitory factor and adhesion molecules
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作者 Eun-Yeong Kim Tae-Wook Chung +7 位作者 Hee-Jung Choi Yeon-Seop Jung Syng-Ook Lee Jun-Yong Choi Hyung Sik Kim Sooseong You Myeong Soo Lee Ki-Tae Ha 《中医杂志:英文版》 SCIE CAS CSCD 2019年第1期15-25,共11页
OBJECTIVE: To find out the combination of the extracts from Paeonia lactiflora Pallas(PL), Rehmannia Glutinosa var. Purpurea Makino(RG), Perilla Frutescens var. Acuta Kudo(PF) to increase endometrial receptivity.METHO... OBJECTIVE: To find out the combination of the extracts from Paeonia lactiflora Pallas(PL), Rehmannia Glutinosa var. Purpurea Makino(RG), Perilla Frutescens var. Acuta Kudo(PF) to increase endometrial receptivity.METHODS: Herbal medicines were extracted with boiling water and polysaccharides were removed.We examined the effect of PL, RG, and PF(PRP), a most effective herbal formula deduced from constitutive ingredient herbs of Antai Yin which is composed of PRP, on the leukemia inhibitory factor(LIF) expression and endometrial receptivity.RESULTS: The combination of the extracts from PRP induced the LIF expression in Ishikawa cells and increased the adhesion between Ishikawa and JAr cells. In addition, PRP-induced attachment of JAr cells onto Ishikawa cells and expression of adhesion molecules, ITGAV, ITGB5, CD44 s, and Lselectin, are significantly reduced by knock-down of LIF expression.CONCLUSION: Induced by the combination of the PRP extracts, the adhesion between trophoblast and endometrial cells are mediated by expression of LIF and adhesion molecules. Thus, we suggest the combination of the PRP extracts may be a novel therapy for enhancing embryo implantation rate. 展开更多
关键词 Paeonia REHMANNIA PERILLA Embryo implantation ENDOMETRIUM Leukemia inhibitory factor Cell adhesion molecules
刀鲚胚胎及胚后发育早期脂肪酸组成变化
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作者 施永海 刘永士 +3 位作者 严银龙 张海明 谢永德 于爱清 《动物学杂志》 CAS CSCD 北大核心 2019年第3期414-424,共11页
为掌握刀鲚(Coilia nasus)胚胎及胚后发育早期的脂肪酸变化规律,采用生化分析手段对刀鲚的胚胎(原肠期,受精后7~9 h)、0日龄仔鱼(初孵仔鱼)、3日龄仔鱼和5日龄仔鱼(开口前)的脂肪酸组成和含量进行了检测分析。结果显示,刀鲚发育早期的... 为掌握刀鲚(Coilia nasus)胚胎及胚后发育早期的脂肪酸变化规律,采用生化分析手段对刀鲚的胚胎(原肠期,受精后7~9 h)、0日龄仔鱼(初孵仔鱼)、3日龄仔鱼和5日龄仔鱼(开口前)的脂肪酸组成和含量进行了检测分析。结果显示,刀鲚发育早期的总脂占干物质的相对含量均较高(53.10%~60.97%),干物质的总脂相对含量随个体发育显著降低,单个个体的总脂含量随个体发育呈现剧烈下降趋势,数值从胚胎的43.62μg/ind剧烈下降到5日龄仔鱼的16.27μg/ind;水分含量随个体发育而升高。刀鲚发育早期上述4个时期的干样中检出6种饱和脂肪酸(SFA)、4种单不饱和脂肪酸(MUFA)和8种多不饱和脂肪酸(PUFA)。4个发育时期脂肪酸相对含量,C18:1n9c占绝对优势(50.39%~57.00%),C16:1丰富且稳定(13.77%~14.24%),C16:0也较丰富(7.45%~9.15%)。单不饱和脂肪酸(MUFA)比例占绝对优势(65.14%~72.26%),n-3与n-6系列多不饱和脂肪酸含量的比值(∑n3PUFA/∑n6PUFA)较小(1.78~2.38)。刀鲚胚胎孵化出膜期间,单个个体单不饱和脂肪酸(MUFA)实际减少程度较高,尤其是C18:1n9c(减少量为13.21μg/ind,减少比例达到55.49%)和C16:1(减少量和比例分别为3.30μg/ind和53.12%),而二十碳五烯酸(EPA)加二十二碳六烯酸(DHA)的减少程度较低(1.44μg/ind和38.41%),尤其是DHA(0.95μg/ind和36.52%)。然而,出膜后,仔鱼对单不饱和脂肪酸(MUFA)的利用相对较低(1.94μg/ind和14.17%),尤其是C18:1n9c(13.21μg/ind和12.41%)和C16:1(0.63μg/ind和21.81%);而对EPA+DHA利用相对较高(1.04μg/ind和45.10%),尤其是DHA(0.71μg/ind和42.61%)。研究表明,刀鲚胚胎优先蓄留EPA和DHA,仔鱼在摄食前大量利用EPA+DHA(特别是DHA),呈现出海水鱼类脂肪酸的利用特点。因此,建议在刀鲚亲本强化培育及产后培育中,增加投喂富含单不饱和脂肪酸(MUFA)(特别是C18:1和C16:1)的饵料,以加强刀鲚亲本的营养积累和产卵后亲本生理机能的恢复;在刀鲚育苗前� 展开更多
关键词 刀鲚 胚胎 仔鱼 脂肪酸
鸡胚注射肉碱对肉鸡孵化率、生长和屠宰性能的影响 预览
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作者 冯云霞 王路 惠永华 《中国饲料》 北大核心 2019年第10期68-71,共4页
文章旨在研究体外鸡胚注射肉碱对肉鸡孵化率及出壳后49 d肉鸡生长性能和屠宰性能的影响。鸡胚在孵化第18天通过羊膜注射0.6、2.4和9.6 mg左旋肉碱/100μL稀释液,此外还有3个对照组(非注射组、穿孔组和注射稀释液组),评估肉鸡孵化率、生... 文章旨在研究体外鸡胚注射肉碱对肉鸡孵化率及出壳后49 d肉鸡生长性能和屠宰性能的影响。鸡胚在孵化第18天通过羊膜注射0.6、2.4和9.6 mg左旋肉碱/100μL稀释液,此外还有3个对照组(非注射组、穿孔组和注射稀释液组),评估肉鸡孵化率、生长期肉鸡死亡率、增重、采食量和料比以及屠宰性能指标(屠宰率、腹脂、小腿肌、大腿肌、鸡翅和胸肌占比)。结果发现:鸡胚注射左旋肉碱对受精蛋孵化率无显著影响(P>0.05),但随左旋肉碱注射量的增加,受精蛋孵化率和仔鸡出壳时间有升高趋势(P=0.08)。鸡胚注射左旋肉碱对出壳后49 d肉鸡生长性能、死亡率及屠宰性能指标均无显著影响(P>0.05)。结论:鸡胚注射0.6、2.4和9.6 mg左旋肉碱/100μL稀释液对出壳后肉鸡生长性能和屠宰性能无显著影响,但可以改善肉鸡出壳时间和受精蛋孵化率。 展开更多
关键词 鸡胚 肉碱 肉鸡 孵化率 生长性能 屠宰性能
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急性镉暴露对斑马鱼早期胚胎发育的毒性效应 预览
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作者 杨瑞瑞 王兰 +1 位作者 孙敏 申萍 《山西农业科学》 2019年第3期351-356,共6页
为研究急性镉暴露对斑马鱼(Danio rerio)早期胚胎发育的毒性效应,设置了6个镉浓度组(0,4.44,8.90,17.80,26.70,35.60μmol/L),分别对2,6,10,24hpf(hour post-fertilization)胚胎进行了检测,分析了镉暴露后各个发育阶段胚胎死亡率和畸形... 为研究急性镉暴露对斑马鱼(Danio rerio)早期胚胎发育的毒性效应,设置了6个镉浓度组(0,4.44,8.90,17.80,26.70,35.60μmol/L),分别对2,6,10,24hpf(hour post-fertilization)胚胎进行了检测,分析了镉暴露后各个发育阶段胚胎死亡率和畸形率的变化,以及MDA含量和SOD活性的改变。结果显示,2hpf的胚胎在不同浓度镉暴露下其死亡率未见明显变化(P>0.05),而6,10,24hpf的胚胎在镉浓度高于8.90μmol/L时其死亡率极显著增加(P<0.01);畸形表型统计分析显示,镉浓度高于8.90μmol/L时,2,6,10,24hpf胚胎畸形率均极显著性升高(P<0.01)。进一步检测2,24hpf胚胎的MDA含量和SOD活性,结果表明,镉暴露后早期胚胎MDA含量均随镉浓度升高逐渐增加,尤其在较高浓度组(17.80,35.60μmol/L)显著高于对照组;而SOD活性随镉浓度升高发生显著变化。由此推断,急性镉暴露对早期斑马鱼胚胎发育具有较强的毒性作用,并造成了严重的氧化损伤。 展开更多
关键词 斑马鱼 胚胎 氧化损伤
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胚胎期暴露手机辐射对仔鼠淋巴细胞的影响 预览
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作者 高辉 茅碧文 +1 位作者 李琳 田清友 《华北理工大学学报:医学版》 2019年第3期169-173,共5页
①目的 探讨胚胎期暴露手机辐射对仔鼠淋巴细胞的影响。②方法 雌雄小鼠同笼饲养,妊娠后将孕鼠随机分为3组:手机对照组,时分多址辐射组和码分多址辐射组。酶免疫技术测定各组仔鼠外周血T淋巴细胞亚群比例,TUNEL法检测脾脏淋巴细胞凋亡率... ①目的 探讨胚胎期暴露手机辐射对仔鼠淋巴细胞的影响。②方法 雌雄小鼠同笼饲养,妊娠后将孕鼠随机分为3组:手机对照组,时分多址辐射组和码分多址辐射组。酶免疫技术测定各组仔鼠外周血T淋巴细胞亚群比例,TUNEL法检测脾脏淋巴细胞凋亡率,细胞生物学方法测定脾脏淋巴细胞转化率。③结果 小鼠外周血CD3、CD4及CD8T淋巴细胞亚群比例在对照组、时分多址组及码分多址组间差异均不具有显著性(P>0.05)。时分多址组及码分多址组脾细胞凋亡率高于对照组,脾细胞转化率低于对照组,差异具有显著性(P<0.05);时分多址组与码分多址组间脾细胞凋亡率及转化率差异均不具有显著性(P>0.05)。④结论 胚胎期暴露手机辐射对仔鼠T细胞亚群比例无影响,但可促进脾细胞凋亡并抑制其增殖活性。 展开更多
关键词 手机辐射 胚胎期 淋巴细胞
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胚胎小鼠肠道神经元的分离和原代培养
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作者 李娜 程波 +4 位作者 李岩松 袁伟 苗继文 王强 李爽 《神经解剖学杂志》 CAS CSCD 北大核心 2019年第2期182-186,共5页
目的:建立一种简便的胚胎小鼠肠道神经元分离和培养方法。方法:分离12~13 d胎鼠肠道组织,制备单细胞悬液,用神经元专用培养基进行培养,观察神经元的生长情况。培养第3、5、7、9、14 d使用免疫细胞化学染色法,以β微管蛋白(Tu J1)反映神... 目的:建立一种简便的胚胎小鼠肠道神经元分离和培养方法。方法:分离12~13 d胎鼠肠道组织,制备单细胞悬液,用神经元专用培养基进行培养,观察神经元的生长情况。培养第3、5、7、9、14 d使用免疫细胞化学染色法,以β微管蛋白(Tu J1)反映神经元突起形态并用Sholl分析进行量化,以神经元核心抗原(Neu N)为标志物反映神经元的纯度。结果:培养24 h神经元贴壁,随培养天数延长神经元突起长度增加,数目增多,形态逐渐复杂,与邻近细胞突起相互连接,形成网络。与培养第3 d相比,第7、9、14 d神经元纯度明显增高(P <0.05)。结论:本方法分离培养的神经元纯度高,在神经元专用培养基中存活状态良好,未见胶质细胞明显增生。 展开更多
关键词 肠道神经元 胚胎 原代培养 小鼠
Impact assessment of modified clay on embryo-larval stages of turbot Scophthalmus maximus L. 预览
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作者 ZHANG Yue SONG Xiuxian +3 位作者 YU Zhiming ZHANG Peipei CAO Xihua YUAN Yongquan 《海洋湖沼学报(英文)》 SCIE CAS CSCD 2019年第3期1051-1061,共11页
Using modified clay is one of the most promising methods for the mitigation of harmful algal blooms(HABs).However,the environmental impact of modified clay has become a subject of global concern.In this study,turbot(S... Using modified clay is one of the most promising methods for the mitigation of harmful algal blooms(HABs).However,the environmental impact of modified clay has become a subject of global concern.In this study,turbot(Scophthalmus maximus L.)embryos were used as a model to assess the effect of modified clay on this sensitive stage of fish development.Results show that the 24 and 48h LC 50(median lethal concentrations)of the modified clay were 1.70 and 1.65 g/L,and the safe concentration was 0.47 g/L,which is much higher than the concentration used to treat HAB.The modified clay did not affect significantly the hatchability of turbot embryos but when the concentration exceeded 0.50 g/L,the deformity rate of newly hatched larvae increased significantly.The total length,specific growth rate(SGR)and yolk sac absorption rate of larvae reached their peaks at 0.50 g/L and then gradually decreased as the concentration of modified clay increased.Therefore,a moderate amount of modified clay does not harm the survival and hatching of turbot embryos,or the growth and development of newly hatched larvae. 展开更多
关键词 modified CLAY TURBOT EMBRYO LARVAE SURVIVAL GROWTH
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Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle 预览
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作者 Thiago Martins Guilherme Pugliesi +6 位作者 Mariana Sponchiado Angela M.Gonella-Diaza Oscar A.Ojeda-Rojas Frederich D.Rodriguez Roney S.Ramos Andrea C.Basso Mario Binelli 《畜牧与生物技术杂志:英文版》 SCIE CAS CSCD 2019年第1期56-66,共11页
Background: A major,unresolved issue is how the uterine microenvironment determines pregnancy success in cattle.Before implantation,conceptus development depends on the uterine secretome(i.e.,histotroph).Despite its p... Background: A major,unresolved issue is how the uterine microenvironment determines pregnancy success in cattle.Before implantation,conceptus development depends on the uterine secretome(i.e.,histotroph).Despite its pivotal role,little is known about the dynamics of histotroph synthesis and changes in composition throughout the early diestrus and the relevance to pregnancy establishment.We hypothesize that disturbances on histotroph composition affect the establishment of pregnancy.Aim was to disturb histotroph composition at early diestrus and verify the effects on:(Exp.1) timing to restore its composition;and(Exp.2) pregnancy rate after multiple-embryo transfer.Estrous cycle of multiparous Nelore cows were synchronized and estrus was considered d 0(D0) of the experiments.Disturbance was through flushing each uterine horn with 30 m L of DMPBS and collecting the resulting uterine luminal flushing(ULF) on D1;D4;D7;D1 + D4 + D7.Control group remained not-collected.In Exp.1,ULF was collected on D7.5 from all animals and used for quantification of total protein concentration and abundance of albumin.In Exp.2,three in vitro-produced embryos were transferred to the uterine horn ipsilateral to the ovary containing the CL on D7.5 and pregnancy was checked on D25 by ultrasound.Results: In Exp.1,ULF collection on D4 or D7 increased(1.5-to 2.2-folds) the total protein concentration and albumin abundance.ULF collection on D1 did not alter(P > 0.10) these endpoints.In Exp.2,ULF collected on D4 or D7 decreased pregnancy rates to approximately half of that measured in the remaining groups.Conclusions: Subtle perturbations imposed to the native intrauterine milieu,such as those caused by a single,low-volume collection of ULF,profoundly disturbs intrauterine composition and pregnancy success.At least 4 d were necessary for the uterus to recover its composition and the functional capacity to carry post-implantation gestation. 展开更多
关键词 ALBUMIN EMBRYO Histotroph Protein
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利用CRISPR-Cas9基因编辑技术制备牛MSTN基因编辑胚胎 预览
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作者 尉翔栋 吕晨晨 +7 位作者 朱肖亭 冯亚杰 辛晓玲 施巧婷 梁瑞清 徐照学 王二耀 滑留帅 《河南农业科学》 北大核心 2019年第2期131-136,共6页
为了给双肌肉牛品种的培育提供新的遗传素材,拟利用CRISPR-Cas9基因编辑技术制备牛MSTN基因编辑胚胎。从采集到的86对牛卵巢中收集卵母细胞进行成熟培养以及体外受精,并使用在线软件设计能够在体外高效编辑牛MSTN基因的gRNA序列,将验证... 为了给双肌肉牛品种的培育提供新的遗传素材,拟利用CRISPR-Cas9基因编辑技术制备牛MSTN基因编辑胚胎。从采集到的86对牛卵巢中收集卵母细胞进行成熟培养以及体外受精,并使用在线软件设计能够在体外高效编辑牛MSTN基因的gRNA序列,将验证后所得的体外切割活性最高的gRNA与Cas9mRNA的混合物显微注射牛的受精卵,培养至囊胚后,利用T7EI核酸内切酶法检测囊胚中MSTN基因的编辑情况,然后将基因编辑阳性的囊胚样品进行测序验证。结果表明,牛胚胎体外培养平均囊胚率为21.28%;在设计的多条gRNA序列中,gRNA5的体外切割活性最高,为96.00%;将Cas9mRNA和gRNA5的混合物显微注射受精卵并培养至囊胚后,T7EI核酸内切酶检测表明囊胚MSTN基因切割阳性率为15.40%;对MSTN基因编辑囊胚阳性样品测序表明,在gRNA5靶位点存在部分片段缺失。综上,说明利用CRISPR-Cas9基因编辑技术成功获得了牛MSTN基因编辑胚胎。 展开更多
关键词 MSTN基因 基因编辑 CRISPR-Cas9 胚胎
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胚胎碎片对早期胚胎发育影响的研究
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作者 许鹏宇 李冬秀 +2 位作者 梁军 张蕾 赵郡 《现代妇产科进展》 CSCD 北大核心 2019年第5期339-341,共3页
目的:探讨胚胎碎片对早期胚胎发育的影响。方法:回顾分析3PN胚胎的囊胚形成率,根据胚胎碎片含量分为A组(<10%)、B组(25%>碎片≥10%)、C组(50%>碎片≥25%)、D组(≥50%),检测各组活性氧(ROS)的含量。选取碎片含量10%~50%的胚胎,... 目的:探讨胚胎碎片对早期胚胎发育的影响。方法:回顾分析3PN胚胎的囊胚形成率,根据胚胎碎片含量分为A组(<10%)、B组(25%>碎片≥10%)、C组(50%>碎片≥25%)、D组(≥50%),检测各组活性氧(ROS)的含量。选取碎片含量10%~50%的胚胎,根据是否行显微碎片清除分为实验组(行显微去碎片)和对照组(未行显微去碎片),观察囊胚形成率。结果:D组的囊胚形成率显著低于A、B、C组,差异有统计学意义(P<0.05)。随着碎片增多,胚胎的ROS逐渐增多,B组和C组间无显著差异,其余各组间有显著差异(P<0.05)。去除碎片后,囊胚形成率为27.70%,显著高于对照组,差异有统计学意义(P<0.05)。结论:ROS增加可能是胚胎碎片形成的主要因素之一,去除碎片可提高囊胚形成率,但去除碎片对胚胎发育潜能的远期影响,有待进一步研究。 展开更多
关键词 胚胎 碎片 形态学评分 发育潜能 活性氧
Acupuncture Enhances Chances of Pregnancy in Unexplained Infertile Patients Who Undergo A Blastocyst Transfer in A Fresh-Cycle
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作者 Hakan Coksüer Mert Ula Barut +4 位作者 Murat BozkurT Elif Agacayak Sibel Sak Mustafa Demir Eray Caliskan 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第4期298-302,共5页
Objective: To analyze the effects of acupuncture on in vitro fertilization patients with unexplained infertility.Methods: We retrospectively analyzed the charts of a total of 302 cycles performed in 273 patients atten... Objective: To analyze the effects of acupuncture on in vitro fertilization patients with unexplained infertility.Methods: We retrospectively analyzed the charts of a total of 302 cycles performed in 273 patients attending Assisted Reproductive Technology(ART) Centre from August 2013 to August 2016.During the study period,embryo transfer with acupuncture(Acupuncture group,46 cases) and without acupuncture(Control group,42 cases) were applied.Prior to embryo transfer,the following points were used in the acupuncture group: Neiguan(CX 6),Diji(SP 8),Taichong(Liv 3),Baihui(Gv 20),and Guilai(S 29).These sessions were carried out two times before and after embryo transfer in a single day.In addition,auricular acupuncture was also performed at ear points,including ear point 55(Shenmen),ear point 58(Zhigong),ear point 22(Neifenmi)and ear point 34(Naodian).The biochemical pregnancy rate and clinical pregnancy rate after transplantation were compared between two groups.Results: The clinical pregnancy rate in the acupuncture group was higher than that in the control group [60.9%(28/46) vs.33.3%(14/42),respectively,P<0.05].Likewise,the live birth rate in the acupuncture group was also higher than that in the control group [71.7%(33/46) vs.31.0%(12/42),P<0.01).Conclusion: Administration of acupuncture on the day of embryo transfer dramatically improved fertility results in women who underwent in vitro fertilization/intra-cytoplasm sperm injection for reproduction. 展开更多
关键词 ACUPUNCTURE assisted REPRODUCTION EMBRYO transfer PREGNANCY rate
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