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Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats 预览
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作者 Chary M. Batista Eric D. Mariano +6 位作者 Camila S. Dale Alexandre F. Cristante Luiz R. Britto Jose P. Otoch Manoel J. Teixeira Matthias Morgalla Guilherme Lepski 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期2011-2019,共9页
Neuropathic pain after spinal cord injury(SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy;nevertheless, the ideal cell type in terms of neuroge... Neuropathic pain after spinal cord injury(SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy;nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells(fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium(sham) or fNSCs extracted from telencephalic vesicles(TV group) or the ventral medulla(VM group) of E/14 embryos. Sensory(von Frey filaments and hot plate) and motor(the Basso, Beattie,Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and VM groups at 4 and 5 weeks after transplantation, respectively. Moreover, mechanical allodynia improved in both the TV and VM groups at 8 weeks. No significant motor recovery was observed in the TV or VM groups compared with sham. Stereological analyses showed that ~70% of TV and VM cells differentiated into NeuN+ neurons,with a high proportion of enkephalinergic and GABAergic cells in the TV group and enkephalinergic and serotoninergic cells in the VM group. Our study suggests that neuronal precursors from TV and VM, once implanted into the injured spinal cord, maturate into different neuronal subtypes, mainly GABAergic, serotoninergic, and enkephalinergic, and all subtypes alleviate pain, despite no significant motor recovery. The study was approved by the Animal Ethics Committee of the Medical School of the University of S?o Paulo(protocol number 033/14) on March 4, 2016. 展开更多
关键词 spinal cord injuries chronic PAIN neural stem cells cell TRANSPLANTATION neuronal differentiation GABAERGIC NEURON serotoninergic NEURON enkephalinergic NEURON
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趋化因子与抑郁症关系的研究进展 预览
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作者 郑清炼 楚世峰 +2 位作者 任倩 许鑫 陈乃宏 《中国药理学通报》 CAS CSCD 北大核心 2019年第5期615-619,共5页
炎症反应是抑郁症的重要发病机制之一。近年来研究表明,许多抑郁症患者的相应脑区都存在炎症反应,而介导炎症反应的趋化因子在其中发挥着重要的作用。趋化因子也与抑郁症涉及的神经可塑性、单胺类神经递质减少及神经内分泌系统密切相关... 炎症反应是抑郁症的重要发病机制之一。近年来研究表明,许多抑郁症患者的相应脑区都存在炎症反应,而介导炎症反应的趋化因子在其中发挥着重要的作用。趋化因子也与抑郁症涉及的神经可塑性、单胺类神经递质减少及神经内分泌系统密切相关。该文对近年来趋化因子与抑郁症关系的研究进展进行简要综述,以期为深入了解和治疗抑郁症带来新的启示。 展开更多
关键词 趋化因子 趋化因子受体 抑郁症 炎症反应 神经可塑性 神经元 小胶质细胞
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Sestrin2过表达在大鼠海马神经元缺氧复氧损伤中的保护作用及其机制研究
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作者 王秀芳 赫建帅 +4 位作者 赵芹 李玲玉 唐莹 黄林 王士雷 《中华神经医学杂志》 CAS CSCD 北大核心 2019年第8期779-784,共6页
目的探讨Sestrin2过表达在大鼠海马神经元缺氧复氧损伤中的保护作用及其机制。方法将从出生24 h内的SD大鼠中提取并体外培养的原代海马神经元以2×10^4个/mL的密度接种到6孔板后,分为对照组、缺氧复氧组、空载体组、Sestrin2过表达... 目的探讨Sestrin2过表达在大鼠海马神经元缺氧复氧损伤中的保护作用及其机制。方法将从出生24 h内的SD大鼠中提取并体外培养的原代海马神经元以2×10^4个/mL的密度接种到6孔板后,分为对照组、缺氧复氧组、空载体组、Sestrin2过表达组,后3组采用氧糖剥夺法制备成缺氧复氧模型,后2组于缺氧复氧处理前分别转染含空载体及Sestrin2过表达基因的慢病毒。缺氧6 h复氧20 h后采用活性氧(ROS)检测试剂盒检测各组海马神经元中ROS含量;采用ATP检测试剂盒检测ATP浓度;采用流式细胞术检测细胞凋亡率;采用Western blotting检测Sestrin2、动力相关蛋白1(Drp1)、Fis1、Bcl-2、Bax、细胞色素C(Cyt C)的蛋白表达,并计算Bcl-2/Bax蛋白比值;采用透射电镜观察线粒体的超微结构。结果与对照组相比,缺氧复氧组海马神经元中ATP浓度、Bcl-2蛋白表达、Bcl-2/Bax蛋白比值明显降低[(11.15±0.42) nmol/mg蛋白vs.(5.30±0.39) nmol/mg蛋白;2.20±0.26 vs. 0.91±0.02;6.46±0.41 vs. 1.04±0.05],ROS平均荧光强度、细胞凋亡率及Sestrin2、Drp1、Fis1、Bax、Cyt C蛋白表达明显增高(152.41±17.38 vs. 1530.00±14.69;3.77%±0.74% vs. 56.57%±1.35%;0.66±0.06 vs. 1.11±0.20;0.48±0.03 vs. 1.16±0.07;1.14±0.09 vs. 2.47±0.09;0.34±0.03 vs. 0.88±0.04;0.17±0.03 vs. 0.30±0.03),差异均有统计学意义(P<0.05),且线粒体的完整性明显破坏。与缺氧复氧组相比,Sestrin2过表达组海马神经元中ATP浓度及Sestrin2、Bcl-2蛋白表达、Bcl-2/Bax蛋白比值明显增高[(5.30±0.39) nmol/mg vs.(8.95±0.27) nmol/mg;1.11±0.20 vs. 2.67±0.07;0.91±0.02 vs. 1.80±0.19;1.04±0.05 vs. 3.95±0.28],ROS平均荧光强度、细胞凋亡率及Drp1、Fis1、Bax、Cyt C蛋白表达明显降低(1530.00±14.69 vs. 337.27±15.32;56.57%±1.35% vs. 10.33%±2.60%;1.16±0.07 vs. 0.43±0.02;2.47±0.09 vs. 1.11±0.08;0.88±0.04 vs. 0.45±0.02;0.30±0.03 vs. 0.17±0.02),差异均有统计学意义(P<0.05),且线粒体的结构相对完整。 展开更多
关键词 Sestrin2 缺氧复氧损伤 神经元 脑缺血再灌注损伤 线粒体分裂
Precision medicine in pantothenate kinase-associated neurodegeneration 预览
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作者 Monica Alvarez-Cordoba Marina Villanueva-Paz +6 位作者 Irene Villalon-Garcia Suleva Povea-Cabello Juan M.Suarez-Rivero Marta Talaveron-Rey Javier Abril-Jaramillo Ana Belen Vintimilla-Tosi Jose A.Sanchez-Alcazar 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1177-1185,共9页
Neurodegeneration with brain iron accumulation is a broad term that describes a heterogeneous group of progressive and invalidating neurologic disorders in which iron deposits in certain brain areas,mainly the basal g... Neurodegeneration with brain iron accumulation is a broad term that describes a heterogeneous group of progressive and invalidating neurologic disorders in which iron deposits in certain brain areas,mainly the basal ganglia.The predominant clinical symptoms include spasticity,progressive dystonia,Parkinson’s disease-like symptoms,neuropsychiatric alterations,and retinal degeneration.Among the neurodegeneration with brain iron accumulation disorders,the most frequent subtype is pantothenate kinase-associated neurodegeneration(PKAN)caused by defects in the gene encoding the enzyme pantothenate kinase 2(PANK2)which catalyzed the first reaction of the coenzyme A biosynthesis pathway.Currently there is no effective treatment to prevent the inexorable course of these disorders.The aim of this review is to open up a discussion on the utility of using cellular models derived from patients as a valuable tool for the development of precision medicine in PKAN.Recently,we have described that dermal fibroblasts obtained from PKAN patients can manifest the main pathological changes of the disease such as intracellular iron accumulation accompanied by large amounts of lipofuscin granules,mitochondrial dysfunction and a pronounced increase of markers of oxidative stress.In addition,PKAN fibroblasts showed a morphological senescence-like phenotype.Interestingly,pantothenate supplementation,the substrate of the PANK2 enzyme,corrected all pathophysiological alterations in responder PKAN fibroblasts with low/residual PANK2 enzyme expression.However,pantothenate treatment had no favourable effect on PKAN fibroblasts harbouring mutations associated with the expression of a truncated/incomplete protein.The correction of pathological alterations by pantothenate in individual mutations was also verified in induced neurons obtained by direct reprograming of PKAN fibroblasts.Our observations indicate that pantothenate supplementation can increase/stabilize the expression levels of PANK2 in specific mutations.Fibroblasts and induced neuro 展开更多
关键词 NEURODEGENERATION with brain iron accumulation pantothenate kinase-associated NEURODEGENERATION pantothenate kinase 2 pantothenate INDUCED neurons PRECISION MEDICINE INDUCED NEURON FIBROBLAST
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高血糖经抑制磷酸化腺苷酸活化蛋白激酶加重脑缺血再灌注损伤 预览
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作者 肖亚利 马艳梅 +3 位作者 郭永真 李雅琼 张建忠 景丽 《解剖学杂志》 CAS 2019年第2期132-137,共6页
目的:探讨磷酸化的腺苷酸活化蛋白激酶(p-AMPK)在高血糖加重脑缺血再灌注损伤中的作用。方法:通过注射链脲佐菌素制备糖尿病高血糖模型,线栓法制备大脑中动脉阻塞(MCAO)再灌注模型;分为假手术组(sham组),正常血糖脑缺血再灌注24h组(NMI/... 目的:探讨磷酸化的腺苷酸活化蛋白激酶(p-AMPK)在高血糖加重脑缺血再灌注损伤中的作用。方法:通过注射链脲佐菌素制备糖尿病高血糖模型,线栓法制备大脑中动脉阻塞(MCAO)再灌注模型;分为假手术组(sham组),正常血糖脑缺血再灌注24h组(NMI/R24h组)、72h组(NMI/R72h组),高血糖脑缺血再灌注24h组(HMI/R24h组)以及72h组(HMI/R72h组),采用组织学、免疫组织化学及免疫印迹等方法,比较观察脑组织的损伤及p-AMPK的表达。结果:NMI/R24h组、NMI/R72h组大鼠均可见神经功能缺失的表现,HM组大鼠神经功能缺失评分明显高于NM组。NMI/R24h组梗死区脑组织疏松水肿,神经元固缩;HM组大鼠脑组织疏松水肿及固缩神经元较NM组大鼠明显增加;与NMI/R72h组比较,HM组大鼠仍存在脑组织疏松水肿,较多固缩神经元。免疫组织化学显色和免疫印迹结果可见,I/R24h和I/R72h,HM组p-AMPK相对蛋白量明显低于NM组。p-AMPK定位观察可见,p-AMPK与神经元共表达,但不与星形胶质细胞共表达。结论:糖尿病高血糖加重大鼠局灶性脑缺血再灌注损伤,可能与神经元磷酸化腺苷酸活化蛋白激酶减少有关。 展开更多
关键词 高血糖 糖尿病 缺血再灌注 神经元 腺苷酸活化蛋白激酶
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不可移动文物风险管理体系构建探讨 预览
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作者 李晓武 杨恒山 向南 《自然与文化遗产研究》 2019年第7期74-85,共12页
风险管理理论已被应用于很多行业,其在金融行业已经建立起成熟的风险管理体系,这为金融行业的业务开展、风险防控发挥了巨大的作用。文章阐述不可移动文物风险管理体系建立的背景及其必要性,提出建立不可移动文物风险管理体系的构想和... 风险管理理论已被应用于很多行业,其在金融行业已经建立起成熟的风险管理体系,这为金融行业的业务开展、风险防控发挥了巨大的作用。文章阐述不可移动文物风险管理体系建立的背景及其必要性,提出建立不可移动文物风险管理体系的构想和基本路径,根据不可移动文物风险管理特点,从风险识别、风险评估、风险防控3个层面进行分析。最后,介绍了一个基于物联网、大数据、云计算、人工智能等现代技术,立足于不可移动文物安全评估的“文物云”平台,结合项目实例,详细剖析其在不可移动文物风险管理方面的具体应用。 展开更多
关键词 不可移动文物 风险管理 风险识别 风险评估 风险防控 智能化 神经元
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心脑舒通胶囊对氧糖剥夺/复氧星形胶质细胞神经营养因子分泌的影响及对神经元的保护作用 预览
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作者 李芮琳 刘文杰 +4 位作者 袁庆 张彤 徐耀 胡利民 柴丽娟 《天津中医药》 CAS 2019年第1期63-66,共4页
[目的]探讨心脑舒通胶囊对氧糖剥夺/复氧(OGD/R)处理的星形胶质细胞(C8-D1A)神经生长因子分泌的影响,以及研究心脑舒通药物处理的OGD/R胶质细胞条件培养液对神经元细胞(Neuro-2A)的保护作用。[方法]CCK-8检测心脑舒通对正常培养和OGD/R... [目的]探讨心脑舒通胶囊对氧糖剥夺/复氧(OGD/R)处理的星形胶质细胞(C8-D1A)神经生长因子分泌的影响,以及研究心脑舒通药物处理的OGD/R胶质细胞条件培养液对神经元细胞(Neuro-2A)的保护作用。[方法]CCK-8检测心脑舒通对正常培养和OGD/R胶质细胞活力的影响;酶联免疫吸附(ELISA)法检测心脑舒通对OGD/R胶质细胞的脑源性神经营养因子(BDNF)、胶质源性神经营养因子(GDNF)、睫状神经营养因子(CNTF)释放的影响;CCK-8检测心脑舒通处理的胶质细胞条件培养液对OGD/R神经元细胞活力的影响。[结果]1)心脑舒通对正常培养的胶质细胞活力无显著影响,但可以提高OGD/R后胶质细胞的增殖活力。2)0.1μg/mL心脑舒通可以显著提高OGD/R胶质细胞BDNF的释放。3)0.1μg/mL心脑舒通药物处理的胶质细胞条件培养液可提高OGD/R神经元的存活能力。[结论]心脑舒通对OGD/R损伤后的胶质细胞有一定保护作用,其处理后的胶质细胞条件培养液对神经元有一定的保护作用。 展开更多
关键词 心脑舒通胶囊 星形胶质细胞 神经元 脑源性神经营养因子 胶质源性神经营养因子
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大鼠皮质神经元的体外培养及不同浓度尿激酶干预后的观察 预览
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作者 葛汝村 吕涌涛 +3 位作者 魏巍 陈平 冯肖亚 徐林 《神经损伤与功能重建》 2019年第6期271-274,共4页
目的:建立科学简易的大鼠皮质神经元的体外培养方法,观察尿激酶对其干预后的表现。方法:取24h内新生Wistar大鼠脑皮质,用浓度为2mg/mL的木瓜酶和适量DNaseI酶共同消化,并用配置好的无血清Neurobasal培养基接种培养,6d后免疫荧光法鉴定... 目的:建立科学简易的大鼠皮质神经元的体外培养方法,观察尿激酶对其干预后的表现。方法:取24h内新生Wistar大鼠脑皮质,用浓度为2mg/mL的木瓜酶和适量DNaseI酶共同消化,并用配置好的无血清Neurobasal培养基接种培养,6d后免疫荧光法鉴定神经元纯度;分别配置含尿激酶终浓度为5000U/mL、8000U/mL、10000U/mL、15000U/mL和20000U/mL的Neurobasal培养基并进行全量换液,镜下观察不同浓度尿激酶干预后神经元的变化。结果:培养6d,神经元分化成熟,胞质丰富,树突及轴突舒展延长,可见密集的神经纤维网络,免疫荧光鉴定神经元纯度为88.2%;尿激酶干预后,发现尿激酶浓度在5000~10000U/mL时,2h内镜下观察培养体系无明显变化,但随时间的延长,尿激酶浓度为10000U/mL作用4h时,可见少量神经元细胞破碎崩解,细胞间网状结构减少。尿激酶浓度在15000U/mL及20000U/mL时,干预2h发现神经元细胞崩解,网状结构消失。结论:本实验建立了一种简易、高效的体外培养新生大鼠皮质神经元的方法,尿激酶浓度在5000~10000U/mL时,2h内对体外神经元培养体系是安全的。 展开更多
关键词 神经元 细胞培养 培养基 免疫荧光 尿激酶
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α-突触核蛋白在大鼠脊髓损伤后的表达及意义 预览
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作者 钟占琼 薛清彩 +3 位作者 刘晓芬 许明珠 向阳 张晓 《中国医药导刊》 2019年第3期170-176,共7页
目的:探究大鼠脊髓损伤(spinal cord injury,SCI)后α-突触核蛋白(α-synuclein, SNCA)在脊髓中的表达和意义。方法:SD大鼠随机分为对照组和SCI组,采用Allen′s法制备大鼠SCI模型。根据基因芯片分析结果,筛选出目的基因。应用实时定量PC... 目的:探究大鼠脊髓损伤(spinal cord injury,SCI)后α-突触核蛋白(α-synuclein, SNCA)在脊髓中的表达和意义。方法:SD大鼠随机分为对照组和SCI组,采用Allen′s法制备大鼠SCI模型。根据基因芯片分析结果,筛选出目的基因。应用实时定量PCR和免疫组织化学方法分别检测SNCA在脊髓中的定位表达和变化。生物信息学分析预测SNCA相关基因或蛋白。结果:基因芯片结果显示差异下调基因SNCA差异表达倍数2.276,验证结果显示SNCA下调。实时定量PCR显示SNCA的 mRNA在大鼠SCI后3、7、14和28 d的表达量明显降低,与对照组相比,差异均有统计学意义( P <0.001);免疫组织化学结果显示SNCA在正常脊髓后角的神经元突起、神经胶质细胞突起以及前角神经元胞浆中表达,在SCI后7 d和14 d,与对照组相比,SNCA免疫阳性物质明显减少。生物信息学分析结果显示SNCA与神经递质传递、氧化应激和细胞凋亡等有关。结论:SCI后SNCA的mRNA和蛋白表达水平均降低,在脊髓前角和后角的表达明显减少。提示在SCI后SNCA可能参与调控神经元和神经胶质细胞的存活,进一步影响SCI后的运动功能和感觉功能。 展开更多
关键词 脊髓损伤 Α-突触核蛋白 神经元 神经胶质细胞
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Expression and Function of Zinc-α2-Glycoprotein
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作者 Xin Wei Xi Liu +5 位作者 Changhong Tan Lijuan Mo Hui Wang Xi Peng Fen Deng Lifen Chen 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第3期540-550,共11页
Zinc-α2-glycoprotein(ZAG), encoded by the AZGP1 gene, is a major histocompatibility complex I molecule and a lipid-mobilizing factor. ZAG has been demonstrated to promote lipid metabolism and glucose utilization, and... Zinc-α2-glycoprotein(ZAG), encoded by the AZGP1 gene, is a major histocompatibility complex I molecule and a lipid-mobilizing factor. ZAG has been demonstrated to promote lipid metabolism and glucose utilization, and to regulate insulin sensitivity. Apart from adipose tissue, skeletal muscle, liver, and kidney, ZAG also occurs in brain tissue, but its distribution in brain is debatable. Only a few studies have investigated ZAG in the brain. It has been found in the brains of patients with Krabbe disease and epilepsy, and in the cerebrospinal fluid of patients with Alzheimer disease, frontotemporal lobe dementia, and amyotrophic lateral sclerosis. Both ZAG protein and AZGP1 m RNA are decreased in epilepsy patients and animal models, while overexpression of ZAG suppresses seizure and epileptic discharges in animal models of epilepsy, but knowledge of the specific mechanism of ZAG in epilepsy is limited. In this review, we summarize the known roles and molecular mechanisms of ZAG in lipid metabolism and glucose metabolism, and in the regulation of insulin sensitivity, and discuss the possible mechanisms by which it suppresses epilepsy. 展开更多
关键词 Zinc-α2-glycoprotein METABOLISM GLUCOSE LIPID INSULIN sensitivity NEURON
外源性脑源神经营养因子降低糖尿病大鼠痛阈的机制研究 预览
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作者 于婷 成洪聚 +2 位作者 辛青 许飞 李雷 《济宁医学院学报》 2019年第4期238-243,共6页
目的探讨外源性脑源神经营养因子(BDNF)及其高亲和力受体酪氨酸激酶受体B(TrkB)对糖尿病周围神经病变(DPN)大鼠的痛觉过敏症状的作用及其机制。方法利用链脲佐菌素(STZ)建立DPN模型大鼠,将DPN模型大鼠随机分为BDNF组、TrkB Fc组和DPN组... 目的探讨外源性脑源神经营养因子(BDNF)及其高亲和力受体酪氨酸激酶受体B(TrkB)对糖尿病周围神经病变(DPN)大鼠的痛觉过敏症状的作用及其机制。方法利用链脲佐菌素(STZ)建立DPN模型大鼠,将DPN模型大鼠随机分为BDNF组、TrkB Fc组和DPN组,每组16只,鞘内置管给予不同药物,BDNF组给予BDNF,TrkB组给予BDNF+TrkB Fc(由TrkB受体的细胞外配体结合结构域组成的合成融合蛋白),DPN组给予DMSO溶剂。用von Frey纤维检测机械痛觉阈值,用热痛仪检测热痛觉阈值。用荧光实时定量PCR检测大鼠背根神经节(DRG)组织BDNF和TrkB的mRNA表达,蛋白印迹检测BDNF和TrkB蛋白表达。用全细胞膜片钳电流钳检测DRG神经元静息电位水平、阈电流强度及动作电位频率。结果与DPN组相比较,BDNF组鞘内注射BDNF升高机械痛觉和热痛觉阈值,并且降低DRG神经元的过度兴奋,这些效应可被预先给予TrkB Fc所阻断,差异具有统计学意义(均P<0.05)。与DPN组相比较,鞘内给予BDNF对DRG中BDNF和TrkB表达无显著影响,差异不具有统计学意义(均P>0.05)。结论外源性BDNF通过降低DRG神经元的过度兴奋缓解DPN大鼠的疼痛症状,BDNF可能是潜在的治疗糖尿病性神经病理性疼痛的新型药物。 展开更多
关键词 脑源性神经营养因子 酪氨酸激酶受体B 背根神经节 神经元 糖尿病神经病变
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仙茅苷对阿尔茨海默病大鼠行为学及海马神经元凋亡的影响
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作者 李若淳 曾明燕 +1 位作者 苏艳丽 吴昌学 《中国临床药理学杂志》 CAS CSCD 北大核心 2019年第7期654-656,670共4页
目的探讨仙茅苷对阿尔茨海默病大鼠行为学及海马神经元凋亡的影响。方法将60只老年健康雄性SD大鼠腹腔注射D-半乳糖60 mg·kg-1,qd(连续6周)联合海马注射β-样淀粉蛋白25-35(Aβ25-35) 4 mmol·L-1,qd(第7周),构建阿尔茨海默病... 目的探讨仙茅苷对阿尔茨海默病大鼠行为学及海马神经元凋亡的影响。方法将60只老年健康雄性SD大鼠腹腔注射D-半乳糖60 mg·kg-1,qd(连续6周)联合海马注射β-样淀粉蛋白25-35(Aβ25-35) 4 mmol·L-1,qd(第7周),构建阿尔茨海默病大鼠模型,并随机分为4组:低、中、高剂量实验组(建模期间予以灌胃仙茅苷24,48,72 mg·kg-1,qd),模型组(建模期间予以灌胃等量0. 9%Na Cl);另选15只老年健康雄性SD大鼠灌胃等量0. 9%Na Cl作为对照组。用Morris水迷宫实验检测各组大鼠学习记忆功能,用苏木精-伊红(HE)染色及细胞凋亡原位检测(TUNEL)法分别观察海马区神经元凋亡情况。结果对照组、模型组及低、中、高剂量实验组大鼠穿台次数分别为(15. 12±1. 78),(5. 03±1. 17),(7. 45±1. 36),(9. 79±1. 21)和(12. 33±1. 09)次,模型组分别与低、中、高剂量实验组比较,差异均有统计学意义(均P <0. 05);海马神经元凋亡比例分别(8. 42±2. 13)%,(69. 78±9. 97)%,(38. 14±8. 62)%,(24. 77±5. 43)%和(10. 02±1. 46)%,模型组分别与低、中、高剂量实验组比较,差异均有统计学意义(均P <0. 01)。结论仙茅苷可缓解海马神经元损伤,抑制海马神经元凋亡。 展开更多
关键词 阿尔茨海默病 仙茅苷 海马 神经元 凋亡
Effects of routine rehabilitation therapy combined with low frequency electrical stimulation on monoamine neurotransmitters, NSE, ET-1 and cerebral hemodynamics in children with cerebral palsy
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作者 Sai Xun Pei-Pei Wang +5 位作者 Nuan Yang Mei Hou Jun Chen Dian-Rong Sun Jian-Hui Zhao Li-Ping Lyu 《海南医科大学学报(英文版)》 2019年第5期61-64,共4页
Objective:To investigate the effects of routine rehabilitation therapy combined with low frequency head stimulation on monoamine neurotransmitters, neuron-specific enolase (NSE), endothelin-1 (ET-1) and cerebral hemod... Objective:To investigate the effects of routine rehabilitation therapy combined with low frequency head stimulation on monoamine neurotransmitters, neuron-specific enolase (NSE), endothelin-1 (ET-1) and cerebral hemodynamics in children with cerebral palsy.Methods:From January 2017 to June 2018, 110 children with cerebral palsy were randomly divided into observation group (55 cases) and control group (55 cases). The control group received routine rehabilitation treatment, while the observation group received low-frequency head stimulation on the basis of routine rehabilitation treatment. The changes of dopamine (DA), norepinephrine (NE), serotonin (5-HT), NSE, ET-1 levels and mean blood flow velocity of anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA) were compared in two groups.Results:Before treatment, there was no significant difference in DA, NE and 5-HT levels in two groups. After treatment, DA, NE and 5-HT levels in the observation group were (192.23±22.71) ng/mL, (98.02±11.71) ng/L, (210.07±25.03) ng/L, and in the control group. the DA, NE, 5-HT levels were (147.06±17.02) ng/mL, (83.07±11.15) ng/L, and (171.88±20.45) ng/L, respectively. The DA, NE and 5-HT levels in two groups were higher than those before treatment, and DA, NE and 5-HT levels in the observation group were higher than those in the control group. Before treatment, there was no significant difference in NSE and ET-1 levels between the two groups. After treatment, the NSE and ET-1 levels in the observation group were (7.97±2.07) μg/L and (41.01±10.07) pg/mL, and the NSE and ET-1 levels in the control group were (10.38±3.02) μg/L, (58.46±15.02) pg/mL, respectively. the NSE and ET-1 in two groups were lower than those before treatment, and the NSE and ET-1 of the observation group were lower than the control group. Before treatment, there was no significant difference in mean blood flow velocity between ACA, MCA and PCA. After treatment, the mean blood flow velocities of ACA, MCA, and PCA 展开更多
关键词 Low frequency electrical stimulation MONOAMINE NEUROTRANSMITTER NEURON specific ENOLASE ENDOTHELIN-1 Cerebral HEMODYNAMICS
Aphasia rehabilitation based on mirror neuron theory:a randomized-block-design study of neuropsychology and functional magnetic resonance imaging 预览
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作者 Wen-Li Chen Qian Ye +5 位作者 Si-Cong Zhang Yang Xia Xi Yang Ti-Fei Yuan Chun-Lei Shan Jian-An Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期1004-1012,共9页
When watching someone performs an action,mirror neurons are activated in a way that is very similar to the activation that occurs when actually performing that action.Previous single-sample case studies indicate that ... When watching someone performs an action,mirror neurons are activated in a way that is very similar to the activation that occurs when actually performing that action.Previous single-sample case studies indicate that hand-action observation training may lead to activation and remodeling of mirror neuron systems,which include important language centers,and may improve language function in aphasia patients.In this randomized-block-design experiment,we recruited 24 aphasia patients from,Zhongda Hospital,Southeast University,China.The patients were divided into three groups where they underwent hand-action observation and repetition,dynamic-object observation and repetition,or conventional speech therapy.Training took place 5 days per week,35 minutes per day,for 2 weeks.We assessed language function via picture naming tests for objects and actions and the Western Aphasia Battery.Among the participants,one patient,his wife and four healthy student volunteers underwent functional magnetic resonance imaging to analyze changes in brain activation during hand-action observation and dynamic-object observation.Results demonstrated that,compared with dynamic-object observation,hand-action observation led to greater performance with respect to the aphasia quotient and affiliated naming sub-tests and a greater Western Aphasia Battery test score.The overall effect was similar to that of conventional aphasia training,yet hand-action observation had advantages compared with conventional training in terms of vocabulary extraction and spontaneous speech.Thus,hand-action observation appears to more strongly activate the mirror neuron system compared with dynamic-object observation.The activated areas included Broca’s area,Wernicke’s area,and the supramarginal gyrus.These results suggest that hand-action observation combined with repetition might better improve language function in aphasia patients compared with dynamic-object observation combined with repetition.The therapeutic mechanism of this intervention may be associated wi 展开更多
关键词 nerve REGENERATION mirror NEURON hand-action observation APHASIA REHABILITATION stroke functional magnetic resonance imaging NEUROPSYCHOLOGY neural REGENERATION
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白藜芦醇预处理减轻神经元焦亡及对神经元缺血性损伤的保护作用 预览
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作者 秦灵芝 李玮 +4 位作者 王晓娟 杨红旗 李书剑 黄月 张杰文 《华中科技大学学报:医学版》 CAS CSCD 北大核心 2019年第1期38-41,共4页
目的探讨白藜芦醇(Res)调控炎性相关蛋白--Nod样受体蛋白3(NLRP3)表达干预神经元焦亡的作用和调控机制。方法培养原代神经元至成熟,随机分为空白组(Sham组)、生理盐水组(Con组)和Res预处理组(Res组)。先对Res组和Con组进行预处理72h,再... 目的探讨白藜芦醇(Res)调控炎性相关蛋白--Nod样受体蛋白3(NLRP3)表达干预神经元焦亡的作用和调控机制。方法培养原代神经元至成熟,随机分为空白组(Sham组)、生理盐水组(Con组)和Res预处理组(Res组)。先对Res组和Con组进行预处理72h,再对3组进行氧糖剥夺损伤6h,最后恢复正常培养液培养2h后取材。利用荧光免疫组化法检测NLRP3的表达情况;利用Western blot检测NLRP3的蛋白水平表达变化。利用标准ELISA试剂盒检测IL-1β和IL-18表达情况。结果与Sham组比较,Con组神经元大量死亡,但存活神经元中NLRP3的染色阳性率高,而Res 10μmol/L、50μmol/L和100μmol/L组均有较多神经元存活,且NLRP3染色阳性率较高;与Con组比较,Res组的NLRP3表达显著降低(均P<0.05),且IL-1β和IL-18表达也明显减少(均P<0.05),各组间差异有统计学意义(P<0.05)。结论Res预处理下调NLRP3的表达,并减轻IL-1β和IL-18分泌和释放,发挥抗神经元焦亡的作用。 展开更多
关键词 Nod样受体蛋白3 白藜芦醇 缺氧缺血性损伤 神经元 白细胞介素-1Β
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调控大鼠脊髓损伤轴突修复 预览
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作者 张衍军 王志杰 +7 位作者 王天仪 陈学明 崔利宾 许崧杰 袁鑫 刘亚东 赵鹏 吴启超 《中国康复理论与实践》 CSCD 北大核心 2019年第3期307-313,共7页
目的探索Wistar大鼠脊髓损伤后损伤局部微环境发生改变的分子生物学机制,寻找起关键调控作用的微RNA。方法15只雌性Wistar大鼠随机分为对照组(n=3)和脊髓损伤组(n=12)。脊髓损伤组根据取材时间分为4h、3d、7d、14d四个组,每组3只。使用M... 目的探索Wistar大鼠脊髓损伤后损伤局部微环境发生改变的分子生物学机制,寻找起关键调控作用的微RNA。方法15只雌性Wistar大鼠随机分为对照组(n=3)和脊髓损伤组(n=12)。脊髓损伤组根据取材时间分为4h、3d、7d、14d四个组,每组3只。使用Microarray3.0芯片检测脊髓损伤大鼠损伤局部发生改变的微RNA,运用生物信息学方法论证发挥关键调控作用的微RNA,进行靶基因预测。运用逆转录实时定量聚合酶链反应(RT-qPCR)技术检测miR-20a-3p表达。采用Westernblotting技术检测信号传导子和转录激活子(STAT)3表达量,并分析各组中靶蛋白与目标微RNA表达变化趋势相关性。在神经元中抑制关键候选微RNA,并使用免疫荧光观察靶蛋白表达与轴突生长的关系。结果脊髓损伤标本中miR-20a-3p特征性上调明显。生物信息分析结果显示,STAT3可为miR-20a-3p的靶基因,与其趋势相反。细胞实验结果显示,miR-20a-3p抑制组与对照组相比轴突延长。Western blotting结果显示,与对照组相比,miR-20a-3p抑制组STAT3蛋白表达显著上调。结论脊髓损伤后miR-20a-3p通过调节其序列互补靶基因STAT3表达量来影响神经元轴突的生长。miR-20a-3p上调导致STAT3下调,抑制miR-20a-3p,可促进神经元轴突的再生。 展开更多
关键词 脊髓损伤 神经元 微RNA 治疗靶点 大鼠
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蛋白激酶CK2神经生物学特性及功能研究进展 预览
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作者 陈岳彤(综述) 武菲(审校) 《济宁医学院学报》 2019年第4期279-284,共6页
蛋白激酶CK2是一种普遍存在的多效性丝/苏氨酸蛋白激酶,在非神经细胞中发挥重要作用。近年来研究证实CK2在神经系统中也扮演不可或缺的角色:CK2在大脑中大量表达,底物众多,通过磷酸化底物参与多种信号通路;在神经元中,CK2存在于突触和... 蛋白激酶CK2是一种普遍存在的多效性丝/苏氨酸蛋白激酶,在非神经细胞中发挥重要作用。近年来研究证实CK2在神经系统中也扮演不可或缺的角色:CK2在大脑中大量表达,底物众多,通过磷酸化底物参与多种信号通路;在神经元中,CK2存在于突触和细胞核等多种基质,并证实其参与调制神经元发育与生长、突触形成、突触传递、突触的可塑性、学习与记忆等生理活动;一些神经因子或化合物可通过CK2起着神经营养或保护作用。本文将对CK2的生物学特性及神经生物学功能研究的最新进展作一综述。 展开更多
关键词 酪蛋白激酶 CK2 神经元 突触传递 神经保护
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中脑导水管周围灰质外侧区神经元 P2X7受体激活对胞内钙离子水平的影响 预览
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作者 李鹏涛 李尤艳 肖智 《医学研究生学报》 CAS 北大核心 2019年第6期602-605,共4页
目的P2X7受体活化的主要细胞内信号是钙离子升高,随后通过多种胞内信号转导途径呈现其生理、病理功能的多样性。探讨原代培养的大鼠中脑导水管周围灰质外侧区(lPAG)神经元P2X7受体激活对胞内钙离子水平的影响。方法将原代培养lPAG神经... 目的P2X7受体活化的主要细胞内信号是钙离子升高,随后通过多种胞内信号转导途径呈现其生理、病理功能的多样性。探讨原代培养的大鼠中脑导水管周围灰质外侧区(lPAG)神经元P2X7受体激活对胞内钙离子水平的影响。方法将原代培养lPAG神经元细胞随机分为4组:空白对照组(不加入药物);BzATP组(100μmol/L);A-740003+BzATP组:先加入100nmol/LA-740003孵育10min,再加入10μmol/L的BzATP;BzATP对照组:先加入无钙液孵育20min,再加入BzATP。其中空白对照组、BzATP组、A-740003+BzATP组孵育液均为DMEM/F12培养基,BzATP对照组孵育液为无钙液。激光共聚焦显微镜技术观察:不同浓度BzATP对培养lPAG神经元胞内钙离子变化;A-740003或无钙液预孵育对BzATP诱发的lPAG神经元胞内钙离子变化的影响。结果BzATP呈剂量依赖性升高lPAG神经元胞内钙离子水平;A-740003和无钙液预孵育可抑制BzATP诱发的lPAG神经元胞内钙离子水平升高。激光共聚焦显微镜下观察显示:BzATP组神经元胞内钙离子荧光强度(2.48±1.05)较空白对照组、BzATP对照组、A-740003+BzATP组([1.12±0.03)、(1.09±0.03)、(1.14±0.08)]明显升高(P<0.01)。结论P2X7受体激活后可以升高lPAG神经元胞内钙离子水平且与细胞外钙离子胞内有关。 展开更多
关键词 中脑导水管周围灰质外侧区 神经元 P2X7受体 钙离子
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一种基于数字信号控制的CMOS神经元电路
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作者 程泽军 李彬鸿 +2 位作者 李博 罗家俊 韩郑生 《微电子学与计算机》 北大核心 2019年第1期6-10,15共6页
采用0.18μmCMOS工艺设计了一种由数字信号控制的神经元电路.相比于传统神经元电路,本电路不需要偏置电压,控制简单,便于大规模集成.在数字信号的控制下,本电路可以方便地实现RegularSpiking(RS),FastSpiking(FS),Chattering(CH),Intrin... 采用0.18μmCMOS工艺设计了一种由数字信号控制的神经元电路.相比于传统神经元电路,本电路不需要偏置电压,控制简单,便于大规模集成.在数字信号的控制下,本电路可以方便地实现RegularSpiking(RS),FastSpiking(FS),Chattering(CH),IntrinsicBursting(IB)四种神经元脉冲响应模式.此外本文介绍了一种激励型突触电路,该电路的突触权值可以通过基于脉冲的SpikeDrivenSynapticPlasticity(SDSP)学习机制进行调节.在此基础上本文利用3个神经元与2个突触实现了Pavlov实验,证明了所设计的神经元电路支持SDSP学习规则,可以用于构建神经形态硬件. 展开更多
关键词 神经元 数字控制 突触 电路
Electroacupuncture promotes peripheral nerve regeneration after facial nerve crush injury and upregulates the expression of glial cell-derived neurotrophic factor 预览
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作者 Jing Fei Lin Gao +2 位作者 Huan-Huan Li Qiong-Lan Yuan Lei-Ji Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期673-682,共10页
The efficacy of electroacupuncture in the treatment of peripheral facial paralysis is known,but the specific mechanism has not been clarified.Glial cell-derived neurotrophic factor(GDNF)has been shown to protect neuro... The efficacy of electroacupuncture in the treatment of peripheral facial paralysis is known,but the specific mechanism has not been clarified.Glial cell-derived neurotrophic factor(GDNF)has been shown to protect neurons by binding to N-cadherin.Our previous results have shown that electroacupuncture could increase the expression of N-cadherin mRNA in facial neurons and promote facial nerve regeneration.In this study,the potential mechanisms by which electroacupuncture promotes nerve regeneration were elucidated through assessing the effects of electroacupuncture on GDNF and N-cadherin expression in facial motoneurons of rabbits with peripheral facial nerve crush injury.New Zealand rabbits were randomly divided into a normal group(normal control,n=21),injury group(n=45)and electroacupuncture group(n=45).Model rabbits underwent facial nerve crush injury only.Rabbits in the electroacupuncture group received facial nerve injury,and then underwent electroacupuncture at Yifeng(TE17),Jiache(ST6),Sibai(ST2),Dicang(ST4),Yangbai(GB14),Quanliao(SI18),and Hegu(LI4;only acupuncture,no electrical stimulation).The results showed that in behavioral assessments,the total scores of blink reflex,vibrissae movement,and position of apex nasi,were markedly lower in the EA group than those in the injury group.Hematoxylin-eosin staining of the right buccinator muscle of each group showed that the cross-sectional area of buccinator was larger in the electroacupuncture group than in the injury group on days 1,14 and 21 post-surgery.Toluidine blue staining of the right facial nerve tissue of each group revealed that on day 14 post-surgery,there was less axonal demyelination and fewer inflammatory cells in the electroacupuncture group compared with the injury group.Quantitative real time-polymerase chain reaction showed that compared with the injury group,N-cadherin mRNA levels on days 4,7,14 and 21 and GDNF mRNA levels on days 4,7 and 14 were significantly higher in the electroacupuncture group.Western blot assay displayed that compared wi 展开更多
关键词 NERVE REGENERATION FACIAL paralysis ELECTROACUPUNCTURE glial cell-derived neurotrophic factor N-cadherin crush injury neuronal apoptosis FACIAL neuron NERVE DEMYELINATION neural REGENERATION
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