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Novel miRNA,miR-sc14,promotes Schwann cell proliferation and migration 预览
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作者 Xi-Meng Ji Shan-Shan Wang +5 位作者 Xiao-Dong Cai Xing-Hui Wang Qian-Yan Liu Pan Wang Zhang-Chun Cheng Tian-Mei Qian 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1651-1656,共6页
MicroRNAs refer to a class of endogenous,short non-coding RNAs that mediate numerous biological functions.MicroRNAs regulate various physiological and pathological activities of peripheral nerves,including peripheral ... MicroRNAs refer to a class of endogenous,short non-coding RNAs that mediate numerous biological functions.MicroRNAs regulate various physiological and pathological activities of peripheral nerves,including peripheral nerve repair and regeneration.Previously,using a rat sciatic nerve injury model,we identified many functionally annotated novel microRNAs,including miR-sc14.Here,we used real-time reverse transcription-polymerase chain reaction to examine miR-sc14 expression in rat sciatic nerve stumps.Our results show that miRsc14 is noticeably altered following sciatic nerve injury,being up-regulated at 1 day and diminished at 7 days.EdU and transwell chamber assay results showed that miR-sc14 mimic promoted proliferation and migration of Schwann cells,while miR-sc14 inhiThe study was approved by the Jiangsu Provincial Laboratory Animal Management Committee,China on March 4,2015(approval No.20150304-004).bitor suppressed their proliferation and migration.Additionally,bioinformatic analysis examined potential target genes of miR-sc14,and found that fibroblast growth factor receptor 2 might be a potential target gene.Specifically,our results show changes of miR-sc14 expression in the sciatic nerve of rats at different time points after nerve injury.Appropriately,up-regulation of miR-sc14 promoted proliferation and migration of Schwann cells.Consequently,miR-sc14 may be an intervention target to promote repair of peripheral nerve injury.The study was approved by the Jiangsu Provincial Laboratory Animal Management Committee,China on March 4,2015(approval No.20150304-004). 展开更多
关键词 NERVE REGENERATION novel microRNAs miR-sc14 PERIPHERAL NERVE injury cell PROLIFERATION cell MIGRATION Schwann cells fibroblast growth factor receptor 2 biological functions PERIPHERAL NERVE REGENERATION regulatory mechanisms neural REGENERATION
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Comparative study of microarray and experimental data on Schwann cells in peripheral nerve degeneration and regeneration:big data analysis 预览
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作者 Ulfuara Shefa Junyang Jung 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期1099-1104,共6页
A Schwann cell has regenerative capabilities and is an important cell in the peripheral nervous system.This microarray study is part of a bioinformatics study that focuses mainly on Schwann cells.Microarray data provi... A Schwann cell has regenerative capabilities and is an important cell in the peripheral nervous system.This microarray study is part of a bioinformatics study that focuses mainly on Schwann cells.Microarray data provide information on differences between microarray-based and experiment-based gene expression analyses.According to microarray data,several genes exhibit increased expression(fold change)but they are weakly expressed in experimental studies(based on morphology,protein and mRNA levels).In contrast,some genes are weakly expressed in microarray data and highly expressed in experimental studies;such genes may represent future target genes in Schwann cell studies.These studies allow us to learn about additional genes that could be used to achieve targeted results from experimental studies.In the current big data study by retrieving more than 5000 scientific articles from PubMed or NCBI,Google Scholar,and Google,1016(up-and downregulated)genes were determined to be related to Schwann cells.However,no experiment was performed in the laboratory;rather,the present study is part of a big data analysis.Our study will contribute to our understanding of Schwann cell biology by aiding in the identification of genes.Based on a comparative analysis of all microarray data,we conclude that the microarray could be a good tool for predicting the expression and intensity of different genes of interest in actual experiments. 展开更多
关键词 Schwann cells big data analysis PERIPHERAL NERVE DEGENERATION PERIPHERAL NERVE REGENERATION MICROARRAY matched GENES promising GENES gene ranking
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On the development of optical peripheral nerve interfaces 预览
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作者 Hans E.Anderson Richard F.ff.Weir 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第3期425-436,共12页
Limb loss and spinal cord injury are two debilitating conditions that continue to grow in prevalence.Prosthetic limbs and limb reanimation present two ways of providing affected individuals with means to interact in t... Limb loss and spinal cord injury are two debilitating conditions that continue to grow in prevalence.Prosthetic limbs and limb reanimation present two ways of providing affected individuals with means to interact in the world.These techniques are both dependent on a robust interface with the peripheral nerve.Current methods for interfacing with the peripheral nerve tend to suffer from low specificity,high latency and insufficient robustness for a chronic implant.An optical peripheral nerve interface may solve some of these problems by decreasing invasiveness and providing single axon specificity.In order to implement such an interface three elements are required:(1)a transducer capable of translating light into a neural stimulus or translating neural activity into changes in fluorescence,(2)a means for delivering said transducer and (3)a microscope for providing the stimulus light and detecting the fluorescence change.There are continued improvements in both genetically encoded calcium and voltage indicators as well as new optogenetic actuators for stimulation.Similarly,improvements in specificity of viral vectors continue to improve expression in the axons of the peripheral nerve.Our work has recently shown that it is possible to virally transduce axons of the peripheral nerve for recording from small fibers.The improvements of these components make an optical peripheral nerve interface a rapidly approaching alternative to current methods. 展开更多
关键词 PERIPHERAL NERVE INTERFACES optogenetics OPTICAL neural interface OPTICAL PERIPHERAL NERVE interface GCaMP ArcLight adenoassociated VIRAL vector lentiviral VECTORS VIRAL VECTORS implantable microscopy
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Effect of exogenous spastin combined with polyethylene glycol on sciatic nerve injury 预览
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作者 Yao-Fa Lin Zheng Xie +3 位作者 Jun Zhou Hui-Hao Chen Wan-Wan Shao Hao-Dong Lin 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1271-1279,共9页
Polyethylene glycol can connect the distal and proximal ends of an injured nerve at the cellular level through axonal fusion to avoid Wallerian degeneration of the injured distal nerve and promote peripheral nerve reg... Polyethylene glycol can connect the distal and proximal ends of an injured nerve at the cellular level through axonal fusion to avoid Wallerian degeneration of the injured distal nerve and promote peripheral nerve regeneration.However,this method can only prevent Wallerian degeneration in 10%of axons because the cytoskeleton is not repaired in a timely fashion.Reconstruction of the cytoskeletal trunk and microtubule network has been suggested to be the key for improving the efficiency of axonal fusion.As a microtubule-severing protein,spastin has been used to enhance cytoskeletal reconstruction.Therefore,we hypothesized that spastin combined with polyethylene glycol can more effectively promote peripheral nerve regeneration.A total of 120 male Sprague-Dawley rats were randomly divided into sham,suture,polyethylene glycol,and polyethylene glycol+spastin groups.In suture group rats,only traditional nerve anastomosis of the end-to-end suture was performed after transection of the sciatic nerve.In polyethylene glycol and polyethylene glycol+spastin groups,50μL of polyethylene glycol or 25μL of polyethylene glycol+25μL of spastin,respectively,were injected immediately under the epineurium of the distal suture.Sensory fiber regeneration distance,which was used to assess early nerve regeneration at 1 week after surgery,was shortest in the suture group,followed by polyethylene glycol group and greatest in the polyethylene glycol+spastin group.Behavioral assessment of motor function recovery in rats showed that limb function was restored in polyethylene glycol and polyethylene glycol+spastin groups at 8 weeks after surgery.At 1,2,4 and 8 weeks after surgery,sciatic functional index values and percentages of gastrocnemius muscle wet weight were highest in the sham group,followed by polyethylene glycol+spastin and polyethylene glycol groups,and lowest in the suture group.Masson staining was utilized to assess the morphology of muscle tissue.Morphological changes in skeletal muscle were detectable in suture,polyethylene g 展开更多
关键词 NERVE REGENERATION PERIPHERAL NERVES Wallerian degeneration polyethylene glycol axonal fusion SPASTIN PERIPHERAL NERVE injuries MASSON staining microtubule neural REGENERATION
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Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration 预览
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作者 Yao-Fa Lin Zheng Xie +2 位作者 Jun Zhou Gang Yin Hao-Dong Lin 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2183-2191,共9页
Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this i... Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this is caused by differential expression of genes and proteins during Wallerian degeneration remains unclear. The right tibial nerve and the common peroneal nerve of the same rat were exposed and completely cut through and then sutured in the same horizontal plane. On days 1, 7, 14, and 21 after surgery, 1–2 cm of nerve tissue distal to the suture site was dissected out from the tibial and common peroneal nerves. The differences in gene and protein expression during Wallerian degeneration of the injured nerves were then studied by RNA sequencing and proteomic techniques. In the tibial and common peroneal nerves, there were 1718, 1374, 1187, and 2195 differentially expressed genes, and 477, 447, 619, and 495 differentially expressed proteins on days 1, 7, 14, and 21 after surgery, respectively. Forty-seven pathways were activated during Wallerian degeneration. Three genes showing significant differential expression by RNA sequencing (Hoxd4, Lpcat4 and Tbx1) were assayed by real-time quantitative polymerase chain reaction. RNA sequencing and real-time quantitative polymerase chain reaction results were consistent. Our findings showed that expression of genes and proteins in injured tibial and the common peroneal nerves were significantly different during Wallerian degeneration at different time points. This suggests that the biological processes during Wallerian degeneration are different in different peripheral nerves after injury. The procedure was approved by the Animal Experimental Ethics Committee of the Second Military Medical University, China (approval No. CZ20160218) on February 18, 2016. 展开更多
关键词 NERVE REGENERATION PERIPHERAL NERVES PERIPHERAL NERVE injuries Wallerian degeneration TIBIAL NERVE common PERONEAL NERVE RNA sequencing proteomic real-time quantitative polymerase chain reaction neural REGENERATION
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Systemic IL-1β production as a consequence of corneal HSV-1 infection-contribution to the development of herpes simplex keratitis
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作者 Joan NíGabhann-Dromgoole Ciaran de Chaumont +6 位作者 David Shahnazaryan Siobhán Smith Conor Malone Jaythoon Hassan Cillian F.De Gascun Caroline A.Jefferies Conor C.Murphy 《国际眼科杂志:英文版》 SCIE CAS 2019年第9期1493-1497,共5页
This study sought to identify potential therapeutic targets in herpes simplex keratitis(HSK) patients with active and inactive infection by investigating peripheral cytokine production. Peripheral blood mononuclear ce... This study sought to identify potential therapeutic targets in herpes simplex keratitis(HSK) patients with active and inactive infection by investigating peripheral cytokine production. Peripheral blood mononuclear cells(PBMCs) and serum were prepared from healthy controls and HSK patients during active infection or following treatment(inactive infection). Serum antibody titres were determined by ELISA. Protein expression levels were analysed by Western blot. Cytokine levels were determined by multiplex ELISA. Active corneal herpes simplex virus type 1(HSV-1) infection resulted in significantly elevated peripheral levels of IL-1β in HSK patients compared to healthy controls, and remained significantly increased following treatment. Elevated production of IL-1β in inactive patients was associated with significantly increased levels of IRF3 and STAT1, key proteins involved in promoting anti-viral immune responses. Our data suggest that inflammation persists beyond the period that it is clinically evident and that enhanced peripheral production of IL-1β may have implications for HSV-1 viral clearance in active and inactive HSK patients. 展开更多
关键词 HERPES SIMPLEX virus type 1 HERPES SIMPLEX KERATITIS inflammation peripheral immune response pathogenesis
Periphery, Peripheral Diplomacy and China’s Frontier
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作者 Xing Guangcheng 《和平》 2019年第1期21-32,共12页
China’s frontier is closely related to its surrounding international environment. China’s rise is not only inseparable from the rapidly developing and changing world, but also will profoundly affect the development ... China’s frontier is closely related to its surrounding international environment. China’s rise is not only inseparable from the rapidly developing and changing world, but also will profoundly affect the development and change of the world. China’s rise is inseparable not only from the surrounding international environment, but also profoundly affect the surrounding international environment. It should be pointed out that the process of China’s rise is related to the surrounding international environment, and a series of new situations and factors have emerged in China’s border areas, whether land or sea calls for our great attention. 展开更多
关键词 PERIPHERAL DIPLOMACY China’s FRONTIER PERIPHERY China
Simultaneous enhancement of efficiency and stability of OLEDs with thermally activated delayed fluorescence materials by modifying carbazoles with peripheral groups
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作者 Yunge Zhang Dongdong Zhang +2 位作者 Taiju Tsuboi Yong Qiu Lian Duan 《中国科学:化学英文版》 SCIE EI CAS CSCD 2019年第3期393-402,共10页
Albeit their high efficiencies, the operational stability of the organic light emitting diodes(OLEDs) based on thermally activated delayed fluorescence(TADF) emitters is still far from satisfaction, and few strategies... Albeit their high efficiencies, the operational stability of the organic light emitting diodes(OLEDs) based on thermally activated delayed fluorescence(TADF) emitters is still far from satisfaction, and few strategies have been proposed to improve their stability. Here, we show that by modifying the carbazole unit, one of the most commonly used donors in TADF emitters, with peripheral groups, both the device efficiency and operational stability can be greatly improved. Awell-known TADF molecule-4,5-di(9H-carbazol-9-yl)phthalonitrile(2CzPN) was chosen as the prototype and modified by introducing peripheral tert-butyl and phenyl groups to the 3,6-positions of the carbazole(named 2tBuCzPN and 2PhCzPN, respectively). The introduced groups not only improve the compounds’ electrochemical stabilities referred to the cyclic voltammetry multi-sweep results, but also promote their photoluminescence quantum yields. Furthermore, reduced singlet-triplet energy gaps are observed, leading to the shortened exciton lifetimes which are benefit to suppress the exciton annihilations. Besides, the steric hindrance of introduced phenyl groups can partly restrain the concentration quenching of the TADF emitter. Consequently, OLEDs based on 2tBuCzPN and 2PhCzPN achieved improved maximum external quantum efficiencies(EQEs) of 17.0% and 14.0%, respectively(compared to 8.5% for 2CzPN). Meanwhile, 2PhCzPN based OLED showed reduced roll-off characteristics and a longer lifetime of 7.8 times higher than that of 2CzPN, testifying the effectiveness of subtle modification of the unstable moieties in simultaneous enhancement of efficiency and stability of OLEDs based on TADF emitters. 展开更多
关键词 TADF OLED STABILITY CARBAZOLE PERIPHERAL group
MicroRNAs in blood and cerebrospinal fluid as diagnostic biomarkers of multiple sclerosis and to monitor disease progression 预览
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作者 Bridget Martinez Philip V.Peplow 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期606-619,共14页
Multiple sclerosis is a chronic autoimmune disease of the central nervous system.It is the main cause of non-traumatic neurological disability in young adults.Multiple sclerosis mostly affects people aged 20–50 years... Multiple sclerosis is a chronic autoimmune disease of the central nervous system.It is the main cause of non-traumatic neurological disability in young adults.Multiple sclerosis mostly affects people aged 20–50 years;however,it can occur in young children and much older adults.Factors identified in the distribution of MS include age,gender,genetics,environment,and ethnic background.Multiple sclerosis is usually associated with progressive degrees of disability.The disease involves demyelination of axons of the central nervous system and causes brain and spinal cord neuronal loss and atrophy.Diagnosing multiple sclerosis is based on a patient’s medical history including symptoms,physical examination,and various tests such as magnetic resonance imaging,cerebrospinal fluid and blood tests,and electrophysiology.The disease course of multiple sclerosis is not well correlated with the biomarkers presently used in clinical practice.Blood-derived biomarkers that can detect and distinguish the different phenotypes in multiple sclerosis may be advantageous in personalized treatment with disease-modifying drugs and to predict response to treatment.The studies reviewed have shown that the expression levels of a large number of miRNAs in peripheral blood,serum,exosomes isolated from serum,and cerebrospinal fluid are altered in multiple sclerosis and can distinguish the disease phenotypes from each other.Further studies are warranted to independently validate these findings so that individual or pairs of miRNAs in serum or cerebrospinal fluid can be used as potential diagnostic markers for adult and pediatric multiple sclerosis and for monitoring disease progression and response to therapy. 展开更多
关键词 clinically isolated syndrome CSF disease progression EXOSOMES humans microRNA multiple sclerosis peripheral blood PHENOTYPES serum
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Novel electrospun poly(ε-caprolactone)/type I collagen nanofiber conduits for repair of peripheral nerve injury 预览
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作者 Chun-Ming Yen Chiung-Chyi Shen +5 位作者 Yi-Chin Yang Bai-Shuan Liu Hsu-Tung Lee Meei-Ling Sheu Meng-Hsiun Tsai Wen-Yu Cheng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1617-1625,共9页
Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury.Natural biopolymers have received much attention because of their biocompatibility.To investigate t... Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury.Natural biopolymers have received much attention because of their biocompatibility.To investigate the effects of novel electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits(biopolymer nanofiber conduits)on the repair of peripheral nerve injury,we bridged 10-mm-long sciatic nerve defects with electrospun absorbable biopolymer nanofiber conduits,poly(ε-caprolactone)or silicone conduits in Sprague-Dawley rats.Rat neurologica1 function was weekly evaluated using sciatic function index within 8 weeks after repair.Eight weeks after repair,sciatic nerve myelin sheaths and axon morphology were observed by osmium tetroxide staining,hematoxylin-eosin staining,and transmission electron microscopy.S-100(Schwann cell marker)and CD4(inflammatory marker)immunoreactivities in sciatic nerve were detected by immunohistochemistry.In rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits,no serious inflammatory reactions were observed in rat hind limbs,the morphology of myelin sheaths in the injured sciatic nerve was close to normal.CD4 immunoreactivity was obviously weaker in rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits than in those subjected to repair with poly(ε-caprolactone)or silicone.Rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits tended to have greater sciatic nerve function recovery than those receiving poly(ε-caprolactone)or silicone repair.These results suggest that electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits have the potential of repairing sciatic nerve defects and exhibit good biocompatibility.All experimental procedures were approved by Institutional Animal Care and Use Committee of Taichung Veteran General Hospital,Taiwan,China(La-1031218)on October 2,2014. 展开更多
关键词 poly(ε-caprolactone) type I collagen ELECTROSPINNING SCIATIC NERVE NERVE CONDUIT immunohistostaining walking track analysis peripheral NERVE injury
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Analysis of the characteristics of diabetic peripheral nerve damage by high frequency ultrasound combined with QTT
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作者 Pei Hu Bin Xiao +5 位作者 Rui Xue De-Jiao Sun Zheng-Sen Dong Tian-Xin Hu Jia-Jing Wang Guang-Mei Zheng 《海南医科大学学报(英文版)》 2019年第7期15-20,共6页
Objective: High frequency ultrasound combined with policy thermal perception testing (QTT) was used to analyze the characteristics of nerve damage in diabetic peripheral neuropathy (DPN), and then provided the basis f... Objective: High frequency ultrasound combined with policy thermal perception testing (QTT) was used to analyze the characteristics of nerve damage in diabetic peripheral neuropathy (DPN), and then provided the basis for the clinical prevention, diagnosis and treatment of DPN. Methods: A total of 110 DPN patients admitted to the hospital from December 2015 to May 2018 were selected, and the characteristics of nerve damage in diabetic peripheral neuropathy were analyzed by ultrasound and QTT. Results: In the results of QTT, the abnormal proportion of the CDT, WDT, CPT and HPT of the lower limbs in DPN's were all higher than the upper limbs;The difference of WDT and HPT in the age and course groups of patients in the upper limbs was statistically significant, the abnormal proportion of WDT and HPT in patients aged 60 years or above or with a course of 10 years or more were all above 45%, higher than those in other groups. The patients with larger mean amplitude of glycemic excursions (MAGE) were significantly higher than those with smaller MAGE, regardless of the abnormal proportion of WDT and HPT observed in upper limbs, lower limbs or upper and lower limbs;The results of ultrasonic measurement of nerve cross sectional area showed: the proportion of patients with ulnar nerve and median nerve abnormally thickened in the high age group (≥45 years) and the long course group (>10 years) was significantly higher than that in the low age group (<45 years) and the short course group (<10 years), the proportion of patients with larger MAGE with abnormal enlargement of median nerve was significantly higher than those with smaller MAGE. The thickening of ulnar nerve, median nerve and peroneal nerve was obvious in the patients of MAGE > 4 mmol/L. Conclusion: (1) The main nerve damage in DPN patients was thermal sensation damage, and the lower limb nerve was more vulnerable than the upper limb nerve. (2) The incidence of ulnar and median nerve damage was higher in patients aged 45 years and older or over 10 years of course. 展开更多
关键词 Quantitative thermal PERCEPTION testing Ultrasound DIABETIC PERIPHERAL NEUROPATHY Cross sectional area Mean AMPLITUDE of glycemic EXCURSIONS
外周静脉置入中心静脉导管术后上肢深静脉血栓形成的相关因素分析
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作者 钱亚萍徐新菊 《中国基层医药》 CAS 2019年第2期141-145,共5页
目的探讨引起经外周静脉置入中心静脉导管(Peripherally inserted central catheter,PICC)相关性上肢深静脉血栓形成的相关危险因素。方法选取2014年1月至2018年2月解放军第一一七医院肿瘤内科接受PICC置管的住院患者300例作为研究对象... 目的探讨引起经外周静脉置入中心静脉导管(Peripherally inserted central catheter,PICC)相关性上肢深静脉血栓形成的相关危险因素。方法选取2014年1月至2018年2月解放军第一一七医院肿瘤内科接受PICC置管的住院患者300例作为研究对象,最终确认置管后发生上肢深静脉血栓者30例(血栓组),其余270例则未发现上肢深静脉血栓(非血栓组)。收集患者的一般资料(性别、年龄、体质指数)、置管技术指标(穿刺次数、置管时长、导管尖端位置)、置留血管指标(置管侧肢体、置管静脉、置管静脉内径)、既往史(高血压病史、糖尿病病史、肿瘤病史、肿瘤化疗史、吸烟史、血栓史、PICC或CVP置管史)和实验室检查指标(血小板计数、血浆D-二聚体、血浆纤维蛋白原、凝血酶原时间),以单因素分析找出血栓组和非血栓组差异具有统计学意义的变量,再将这些变量代入logistic回归分析,找出导致PICC置管后上肢深静脉血栓形成的危险因素。结果单因素分析显示,血栓组与非血栓组具有差异性的变量有导管尖端位置、置管静脉内径、肿瘤病史、肿瘤化疗史和血浆D-二聚体(χ^2=4.556、10.973、11.940、5.357、11.623,P=0.033、0.001、0.001、0.021、0.001);多因素logistic回归分析得出,PICC置管术后上肢深静脉血栓形成的危险因素为导管尖端置于上腔静脉上2/3处、置管静脉内径<4mm、既往有肿瘤病史和肿瘤化疗史(P=0.014、0.023、0.017、0.028)。结论PICC置管术后上肢深静脉血栓形成的危险因素为导管尖端置于上腔静脉上2/3处、置管静脉内径<4mm、既往有肿瘤病史和肿瘤化疗史。 展开更多
关键词 导管插入术 中心静脉 导管插入术 外周 腔静脉 血栓形成 危险因素
Keeping nephrotic syndrome on the emergency department edema differential:A case report 预览
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作者 Joshua Goodwin Bijon Das 《世界急诊医学杂志(英文)》 CAS CSCD 2019年第2期116-118,共3页
Dear editor,Nephrotic syndrome is defined by the presence of peripheral edema,heavy proteinuria(greater than 3.5 g/24h),and hypoalbuminemia(less than 3 g/dL).^[1]Nephrotic syndrome is relatively rare,with an incidence... Dear editor,Nephrotic syndrome is defined by the presence of peripheral edema,heavy proteinuria(greater than 3.5 g/24h),and hypoalbuminemia(less than 3 g/dL).^[1]Nephrotic syndrome is relatively rare,with an incidence of 3 new patients per 100,000 per year in adults.^[1]Despite being a known cause for new onset edema in patients at any age,nephrotic syndrome is often neglected in considering differential diagnoses for this presentation in primary care settings,and initial workups often focus on ruling out cardiac and hepatic causes of edema.^[1-3]In this case report,we describe a 25-year-old male patient who presented to the emergency department(ED)complaining of a 10-day history of anasarca.He was later diagnosed with nephrotic syndrome secondary to minimal change disease.This case served as a reminder to include the differential diagnosis of nephrotic syndrome early in the workup of an adult with peripheral edema presenting to the ED. 展开更多
关键词 DEAR EDITOR Nephrotic syndrome PERIPHERAL EDEMA
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Variation in expression of small ubiquitin-like modifiers in injured sciatic nerve of mice 预览
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作者 Dian-Ying Zhang Kai Yu +3 位作者 Zhong Yang Xiao-Zhi Liu Xiao-Fang Ma Yan-Xia Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第8期1455-1461,共7页
Small ubiquitin-like modifiers (SUMOs) have been shown to regulate axonal regeneration, signal transduction, neuronal migration, and myelination, by covalently and reversibly attaching to the protein substrates during... Small ubiquitin-like modifiers (SUMOs) have been shown to regulate axonal regeneration, signal transduction, neuronal migration, and myelination, by covalently and reversibly attaching to the protein substrates during neuronal cell growth, development, and differentiation. It has not been reported whether SUMOs play a role in peripheral nerve injury and regeneration. To investigate any association between SUMOylation and potential neuroprotective effects during peripheral nerve injury and regeneration, C57/BL mice were randomly divided into sham and experimental groups. The sciatic nerve was exposed only in the sham group. The experimental group underwent neurotomy and epineurial neurorrhaphy. Real-time quantitative polymerase chain reaction and western blot assay results revealed different mRNA and protein expression levels of SUMO1, SUMO2, SUM03 and UBC9 in sciatic nerve tissue (containing both 5 mm of proximal and distal stumps at the injury site) at various time points after injury. Compared with the sham group, protein levels of SUM01 and SUMO2/3 increased in both their covalent and free states after sciatic nerve injury in the experimental group, especially in the covalent state. UBC9 protein levels showed similar changes to those of SUMO1 and SUMO2/3 in the covalent states. Immunohistochemical staining demonstrated that SUMO1 and SUMO2/3 immunopositivities were higher in the experimental group than in the sham group. Our results verified that during the repair of sciatic nerve injury, the mRNA and protein expression of SUMO1, SUMO2, SUMO3 and UBC9 in injured nerve tissues changed in varying patterns and there were clear changes in the expression of SUMO-related proteins. These findings reveal that SUMOs possibly play an important role in the repair of peripheral nerve injury. All animal protocols were approved by the Institutional Animal Care and Use Committee of Tianjin Fifth Central Hospital, China (approval No. TJWZXLL2018041) on November & 2018. 展开更多
关键词 NERVE REGENERATION peripheral NERVE injury SCIATIC NERVE SUMO1 SUMO2/3 UBC9 SUMOylation epineurial NEURORRHAPHY neural REGENERATION
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Protective effect of hydrogen sulfide on oxidative stress-induced neurodegenerative diseases 预览
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作者 Rubaiya Tabassum Na Young Jeong Junyang Jung 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期232-241,共10页
Hydrogen sulfide is an antioxidant molecule that has a wide range of biological effects against oxidative stress. Balanced oxidative stress is also vital for maintaining cellular function in biological system, where r... Hydrogen sulfide is an antioxidant molecule that has a wide range of biological effects against oxidative stress. Balanced oxidative stress is also vital for maintaining cellular function in biological system, where reactive oxygen species are the main source of oxidative stress. When the normal redox balance is disturbed, deoxyribonucleic acid, lipid, and protein molecules are oxidized under pathological conditions, like diabetes mellitus that leads to diabetic peripheral neuropathy. In diabetes mellitus-induced diabetic peripheral neuropathy, due to hyperglycemia, pancreatic beta cell(β cell) shows resistance to insulin secretion. As a consequence, glucose metabolism is disturbed in neuronal cells which are distracted from providing proper cell signaling pathway. Not only diabetic peripheral neuropathy but also other central damages occur in brain neuropathy. Neurological studies regarding type 1 diabetes mellitus patients with Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis have shown changes in the central nervous system because high blood glucose levels(HbA1 c) appeared with poor cognitive function. Oxidative stress plays a role in inhibiting insulin signaling that is necessary for brain function. Hydrogen sulfide exhibits antioxidant effects against oxidative stress, where cystathionine β synthase, cystathionine γ lyase, and 3-mercaptopyruvate sulfurtransferase are the endogenous sources of hydrogen sulfide. This review is to explore the pathogenesis of diabetes mellitus-induced diabetic peripheral neuropathy and other neurological comorbid disorders under the oxidative stress condition and the anti-oxidative effects of hydrogen sulfide. 展开更多
关键词 Alzheimer's DISEASE amyotrophic lateral SCLEROSIS antioxidant diabetic peripheral NEUROPATHY DNA oxidation hydrogen SULFIDE mitochondrial dysfunction oxidative stress Parkinson's DISEASE reactive oxygen species
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Relationship between Maternal PBMC HBV cccDNA and HBV Serological Markers and its Effect on HBV Intrauterine Transmission 预览
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作者 WANG Dan Dan YI Lin Zhu +7 位作者 WU Li Na YANG Zhi Qing HAO Hai Yun SHI Xiao Hong WANG Bo FENG Shu Ying FENG Yong Liang WANG Su Ping 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第5期315-323,共9页
Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and... Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing. 展开更多
关键词 Hepatitis B virus INTRAUTERINE transmission Peripheral blood MONONUCLEAR cells Covalently closed circular deoxyribonucleic acid SEROLOGICAL markers
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TrkA regulates the regenerative capacity of bone marrow stromal stem cells in nerve grafts 预览
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作者 Mei-Ge Zheng Wen-Yuan Sui +8 位作者 Zhen-Dan He Yan Liu Yu-Lin Huang Shu-Hua Mu Xin-Zhong Xu Ji-Sen Zhang Jun-Le Qu Jian Zhang Dong Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1765-1771,共7页
We previously demonstrated that overexpression of tropomyosin receptor kinase A(TrkA)promotes the survival and Schwann celllike differentiation of bone marrow stromal stem cells in nerve grafts,thereby enhancing the r... We previously demonstrated that overexpression of tropomyosin receptor kinase A(TrkA)promotes the survival and Schwann celllike differentiation of bone marrow stromal stem cells in nerve grafts,thereby enhancing the regeneration and functional recovery of the peripheral nerve.In the present study,we investigated the molecular mechanisms underlying the neuroprotective effects of TrkA in bone marrow stromal stem cells seeded into nerve grafts.Bone marrow stromal stem cells from Sprague-Dawley rats were infected with recombinant lentivirus vector expressing rat TrkA,TrkA-shRNA or the respective control.The cells were then seeded into allogeneic rat acellular nerve allografts for bridging a 1-cm right sciatic nerve defect.Then,8 weeks after surgery,hematoxylin and eosin staining showed that compared with the control groups,the cells and fibers in the TrkA overexpressing group were more densely and uniformly arranged,whereas they were relatively sparse and arranged in a disordered manner in the TrkA-shRNA group.Western blot assay showed that compared with the control groups,the TrkA overexpressing group had higher expression of the myelin marker,myelin basic protein and the axonal marker neurofilament 200.The TrkA overexpressing group also had higher levels of various signaling molecules,including TrkA,pTrkA(Tyr490),extracellular signal-regulated kinases 1/2(Erkl/2),pErk1/2(Thr202/Tyr204),and the anti-apoptotic proteins Bcl-2 and Bcl-xL.In contrast,these proteins were downregulated,while the pro-apoptotic factors Bax and Bad were upregulated,in the TrkA-shRNA group.The levels of the TrkA effectors Akt and pAkt(Ser473)were not different among the groups.These results suggest that TrkA enhances the survival and regenerative capacity of bone marrow stromal stem cells through upregulation of the Erk/Bcl-2 pathway.All procedures were approved by the Animal Ethical and Welfare Committee of Shenzhen University,China in December 2014(approval No.AEWC-2014-001219). 展开更多
关键词 NERVE REGENERATION bone marrow stromal stem cells TROPOMYOSIN RECEPTOR kinase A RECEPTOR LENTIVIRAL vector shRNA extracellular SIGNAL-REGULATED protein kinases 1/2 Bcl-2 NERVE grafts peripheral NERVE REGENERATION survival neural REGENERATION
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Association between serum uric acid and large-nerve fiber dysfunction in type 2 diabetes:a cross-sectional study
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作者 Tian-Nan Jiang Yu-Feng Li +4 位作者 Li-Li Huo Qian Zhang Lian-Ying Wang Cui-Ling Zhao Li-Ge Liu 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第9期1015-1022,共8页
Background:Large-nerve fiber dysfunction,as assessed by vibration perception threshold (VPT) predicts risks of ulceration,amputation,and mortality in diabetes.Serum uric acid (UA) is closely associated with various me... Background:Large-nerve fiber dysfunction,as assessed by vibration perception threshold (VPT) predicts risks of ulceration,amputation,and mortality in diabetes.Serum uric acid (UA) is closely associated with various metabolic disorders,especially diabetes.Thus,we sought to investigate the clinical relevance of UA to large-nerve fiber dysfunction,among patients with type 2 diabetes (T2D).Methods:Medical records of consecutive patients with T2D who were admitted to Beijing Friendship Hospital Pinggu Campus between May 2014 and December 2016 were collected.Data for the 824 eligible patients included in the final analysis were extracted using a structured form.A VPT value ≥15 in either foot was defined as abnormal.We compared the clinical characteristics between patients with abnormal VPT and those with normal VPT (VPT value <15 in both feet) in the overall population and in gender subgroups.Logistic regression analysis was performed to explore the association of abnormal VPT with UA level.One-way analysis of variance was used to compare VPT values across four UA quartiles.Results:UA levels were significantly lower in T2D patients with abnormal VPT than in those with normal VPT (294.5 ± 84.0 vs.314.9 ± 92.8 μmol/L,P<0.01),especially among male patients (311.7 ± 85.2 vs.336.9 ± 89.6 μmol/L,P<0.01).From the logistic regression analysis,hyperuricemia (males >420 μmol/L;females >360 μmol/L) was associated with a reduced risk of abnormal VPT (odds ratio [OR],0.60;95% confidence interval [CI],0.39–0.91;P<0.05).This association was robust in male patients (OR,0.43;95% CI,0.24–0.76;P<0.01) but not in female patients (OR,0.92;95% CI,0.47–1.82;P = 0.816),even after adjustment for confounding factors.For the younger male subgroup (age <65 years),VPT values decreased as the UA level increased (P for trend = 0.002),but this trend was not significant in older male subgroup (age ≥65 years;P for trend = 0.400).Conclusions:Low serum UA levels showed a significant association with an increased risk of large-nerve fibe 展开更多
关键词 PERIPHERAL NEUROPATHY Uric ACID Type 2 DIABETES
Immunomodulatory properties of dental tissue-derived mesenchymal stem cells:Implication in disease and tissue regeneration 预览
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作者 Oleh Andrukhov Christian Behm +1 位作者 Alice Blufstein Xiaohui Rausch-Fan 《世界干细胞杂志:英文版(电子版)》 2019年第9期604-617,共14页
Mesenchymal stem cells(MSCs)are considered as an attractive tool for tissue regeneration and possess a strong immunomodulatory ability.Dental tissuederived MSCs can be isolated from different sources,such as the denta... Mesenchymal stem cells(MSCs)are considered as an attractive tool for tissue regeneration and possess a strong immunomodulatory ability.Dental tissuederived MSCs can be isolated from different sources,such as the dental pulp,periodontal ligament,deciduous teeth,apical papilla,dental follicles and gingiva.According to numerous in vitro studies,the effect of dental MSCs on immune cells might depend on several factors,such as the experimental setting,MSC tissue source and type of immune cell preparation.Most studies have shown that the immunomodulatory activity of dental MSCs is strongly upregulated by activated immune cells.MSCs exert mostly immunosuppressive effects,leading to the dampening of immune cell activation.Thus,the reciprocal interaction between dental MSCs and immune cells represents an elegant mechanism that potentially contributes to tissue homeostasis and inflammatory disease progression.Although the immunomodulatory potential of dental MSCs has been extensively investigated in vitro,its role in vivo remains obscure.A few studies have reported that the MSCs isolated from inflamed dental tissues have a compromised immunomodulatory ability.Moreover,the expression of some immunomodulatory proteins is enhanced in periodontal disease and even shows some correlation with disease severity.MSC-based immunomodulation may play an essential role in the regeneration of different dental tissues.Therefore,immunomodulation-based strategies may be a very promising tool in regenerative dentistry. 展开更多
关键词 Mesenchymal stem CELLS DENTAL TISSUE IMMUNOMODULATION Peripheral blood MONONUCLEAR CELLS Oral diseases TISSUE regeneration
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Remnant neuromuscular junctions in denervated muscles contribute to functional recovery in delayed peripheral nerve repair 预览
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作者 Leyang Li Hiroyuki Yokoyama +5 位作者 Hidetoshi Kaburagi Takashi Hirai Kunikazu Tsuji Mitsuhiro Enomoto Yoshiaki Wakabayashi Atsushi Okawa 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期731-738,共8页
Schwann cell proliferation in peripheral nerve injury(PNI)enhances axonal regeneration compared to central nerve injury.However,even in PNI,long-term nerve damage without repair induces degeneration of neuromuscular j... Schwann cell proliferation in peripheral nerve injury(PNI)enhances axonal regeneration compared to central nerve injury.However,even in PNI,long-term nerve damage without repair induces degeneration of neuromuscular junctions(NMJs),and muscle atrophy results in irreversible dysfunction.The peripheral regeneration of motor axons depends on the duration of skeletal muscle denervation.To overcome this difficulty in nerve regeneration,detailed mechanisms should be determined for not only Schwann cells but also NMJ degeneration after PNI and regeneration after nerve repair.Here,we examined motor axon denervation in the tibialis anterior muscle after peroneal nerve transection in thy1-YFP mice and regeneration with nerve reconstruction using allografts.The number of NMJs in the tibialis anterior muscle was maintained up to 4 weeks and then decreased at 6 weeks after injury.In contrast,the number of Schwann cells showed a stepwise decline and then reached a plateau at 6 weeks after injury.For regeneration,we reconstructed the degenerated nerve with an allograft at 4 and 6 weeks after injury,and evaluated functional and histological outcomes for 10 to 12 weeks after grafting.A higher number of pretzel-shaped NMJs in the tibialis anterior muscle and better functional recovery were observed in mice with a 4-week delay in surgery than in those with a 6-week delay.Nerve repair within 4 weeks after PNI is necessary for successful recovery in mice.Prevention of synaptic acetylcholine receptor degeneration may play a key role in peripheral nerve regeneration.All animal experiments were approved by the Institutional Animal Care and Use Committee of Tokyo Medical and Dental University on 5 July 2017,30 March 2018,and 15 May 2019(A2017-311C,A2018-297A,and A2019-248A),respectively. 展开更多
关键词 AXON nerve allograft nerve regeneration NEURODEGENERATION neuromuscular junction peripheral nerve injury Schwann cell skeletal muscle
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