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Inhibition of Herpes Simplex Virus-1 Replication by Natural Compound Honokiol
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作者 Shuai Liu Long Li +1 位作者 Lingbing Tan Xiaozhen Liang 《中国病毒学:英文版》 CAS CSCD 2019年第3期315-323,共9页
Honokiol is a pleiotropic natural compound isolated from Magnolia and has multiple biological and clinically relevant effects,including anticancer and antimicrobial function.However,the antiviral activity of honokiol ... Honokiol is a pleiotropic natural compound isolated from Magnolia and has multiple biological and clinically relevant effects,including anticancer and antimicrobial function.However,the antiviral activity of honokiol has not yet been well studied.Here we showed that honokiol had no effect on herpes simplex virus-1(HSV-1)entry,but inhibited HSV-1 viral DNA replication,gene expression and the production of new progeny viruses.The combination of honokiol and clinical drug acyclovir augmented inhibition of HSV-1 infection.Our results illustrate that honokiol could be a potential new candidate for clinical consideration in the treatment of HSV-1 infection alone or combination with other therapeutics. 展开更多
关键词 HONOKIOL HERPES simplex virus-1(HSV-1) Viral REPLICATION ANTI-VIRAL activity ACYCLOVIR
以皮肤损害为首要表现的巨细胞病毒感染一例
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作者 陈怡雯 苏婷 +3 位作者 张美华 崔婷 鲁严 苏忠兰 《中华皮肤科杂志》 CAS CSCD 北大核心 2019年第4期236-240,共5页
报道1例以皮肤损害为首要表现的巨细胞病毒(CMV)感染。患者女,46岁,因口周水疱、糜烂、痛3个月,左臂红斑、水疱、糜烂6周就诊。既往有系统性红斑狼疮、狼疮性肾炎病史12年,5个月前狼疮活动,予糖皮质激素及吗替麦考酚酯治疗。皮肤科检查... 报道1例以皮肤损害为首要表现的巨细胞病毒(CMV)感染。患者女,46岁,因口周水疱、糜烂、痛3个月,左臂红斑、水疱、糜烂6周就诊。既往有系统性红斑狼疮、狼疮性肾炎病史12年,5个月前狼疮活动,予糖皮质激素及吗替麦考酚酯治疗。皮肤科检查:口周见不规则水疱,部分糜烂结痂;左前臂屈侧见一片状红斑,中央糜烂;左上臂见红斑水疱;腹部多发瘀点、瘀斑。皮损组织病理(左上臂):表皮松解坏死,内可见病毒包涵体样结构;免疫组化:表皮松解处大细胞内CMV抗原染色阳性。皮损分泌物CMVDNA阳性,血清CMVDNA初次检测阴性,1周后为8.04×10^3拷贝/ml;血清CMVIgG阳性。诊断:皮肤CMV感染。予口服更昔洛韦及外用后皮疹逐渐好转,但随后出现重症肺部感染,最终患者因多脏器功能衰竭死亡。 展开更多
关键词 巨细胞病毒感染 红斑狼疮 系统性 免疫减弱宿主 包涵体 病毒 病毒载量
On the development of optical peripheral nerve interfaces 预览
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作者 Hans E.Anderson Richard F.ff.Weir 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第3期425-436,共12页
Limb loss and spinal cord injury are two debilitating conditions that continue to grow in prevalence.Prosthetic limbs and limb reanimation present two ways of providing affected individuals with means to interact in t... Limb loss and spinal cord injury are two debilitating conditions that continue to grow in prevalence.Prosthetic limbs and limb reanimation present two ways of providing affected individuals with means to interact in the world.These techniques are both dependent on a robust interface with the peripheral nerve.Current methods for interfacing with the peripheral nerve tend to suffer from low specificity,high latency and insufficient robustness for a chronic implant.An optical peripheral nerve interface may solve some of these problems by decreasing invasiveness and providing single axon specificity.In order to implement such an interface three elements are required:(1)a transducer capable of translating light into a neural stimulus or translating neural activity into changes in fluorescence,(2)a means for delivering said transducer and (3)a microscope for providing the stimulus light and detecting the fluorescence change.There are continued improvements in both genetically encoded calcium and voltage indicators as well as new optogenetic actuators for stimulation.Similarly,improvements in specificity of viral vectors continue to improve expression in the axons of the peripheral nerve.Our work has recently shown that it is possible to virally transduce axons of the peripheral nerve for recording from small fibers.The improvements of these components make an optical peripheral nerve interface a rapidly approaching alternative to current methods. 展开更多
关键词 PERIPHERAL NERVE INTERFACES optogenetics OPTICAL neural interface OPTICAL PERIPHERAL NERVE interface GCaMP ArcLight adenoassociated VIRAL vector lentiviral VECTORS VIRAL VECTORS implantable microscopy
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甲基强的松龙治疗黄疸型病毒性肝炎的疗效 预览
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作者 田立峰 王文平 臧玮 《中国继续医学教育》 2019年第1期127-129,共3页
目的研究甲基强的松龙(甲强龙)治疗黄疸型病毒性肝炎的临床疗效。方法选取我院2017年1月—2018年1月收治的30例黄疸型病毒性肝炎患者为研究对象,根据电脑抽签的方式随机分为观察组与对照组,对照组接受还原型谷胱甘肽治疗方式,观察组接... 目的研究甲基强的松龙(甲强龙)治疗黄疸型病毒性肝炎的临床疗效。方法选取我院2017年1月—2018年1月收治的30例黄疸型病毒性肝炎患者为研究对象,根据电脑抽签的方式随机分为观察组与对照组,对照组接受还原型谷胱甘肽治疗方式,观察组接受甲基强的松龙治疗方式,观察两组患者临床治疗指标及治疗前后TBIL、ALT水平差异。结果两组患者治疗总有效率差异无统计学意义(P>0.05)。TBIL及ALT水平治疗前差异无统计学意义(P>0.05),治疗后观察组显著低于对照组,改善情况显著优于对照组,差异有统计学意义(P<0.05)。结论甲基强的松龙治疗黄疸型病毒性肝炎相较于还原型谷胱甘肽治疗未见差异,但能够更加有效的减轻患者痛苦,促进总胆红素与丙氨酸转氨酶的改善。 展开更多
关键词 甲基强的松龙 还原型谷胱甘肽 黄疸型 病毒性 肝炎 治疗有效率
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Intravenous injection of AAV-PHP.eB across the blood-brain barrier in the adult mouse for central nervous system gene therapy 预览
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作者 Yongwei Shu Jie Yao +7 位作者 Yang Qu Jing Zheng Jing Ding Lina Zhang Yefan Wang Linlin Zhao Jingyu Zhang Siqi Tang 《肿瘤学与转化医学:英文版》 2019年第1期1-5,共5页
Objective To verify the neurotypicality of AAV-PHP.eB after tail vein injection in adult mice and its efficiency in crossing the blood-brain barrier (BBB). Methods The rAAV-SYN-GFP plasmid was constructed, and adult C... Objective To verify the neurotypicality of AAV-PHP.eB after tail vein injection in adult mice and its efficiency in crossing the blood-brain barrier (BBB). Methods The rAAV-SYN-GFP plasmid was constructed, and adult C57BL mice were injected with AAVPHP. eB: SYN-GFP in the tail vein (300 nL, virus titer 3 × 10^9 vg) and in the prefrontal lobe (50 L, virus titer 5 × 10^11 vg). The green fluorescent protein (GFP) signal in the brain was observed at two weeks, while the GFP signal in the peripheral organs was observed at four weeks. Results Two weeks after tail vein injection, GFP expression was observed throughout the brain, especially in the cortex, hippocampus, and geniculate nucleus. No GFP signal was observed or detected by western blotting in the peripheral organs after four weeks. GFP signal was observed mainly at the local site after prefrontal lobe injection. Conclusion AAV-PHP.eB: SYN-GFP can effectively cross the BBB in adult mice. Using a neuron-specific promoter allows exogenous gene expression in neurons;therefore, AAV-PHP.eB can be used as an effective carrier for studying diseases in the central nervous system (CNS). 展开更多
关键词 gene therapy AAV-PHP.eB blood-brain barrier regulatory element NONINVASIVE viral injection
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HIV care continuum among newly diagnosed student and non-student youths between 2012 and 2016 in Hangzhou, China
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作者 Yan Luo Hong Wu +4 位作者 Xing-Liang Zhang Xi-Ting Li Sarah Robbins Scott Jun-Fang Chen Zun-You Wu 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第12期1420-1428,共9页
Background: Youths are disproportionally affected by the human immunodeficiency virus (HIV) infection. We aimed to assess antiretroviral therapy (ART) initiation and viral suppression rates among student and non-stude... Background: Youths are disproportionally affected by the human immunodeficiency virus (HIV) infection. We aimed to assess antiretroviral therapy (ART) initiation and viral suppression rates among student and non-student youths in Hangzhou, China. Methods: Data were taken from the Chinese HIV/acquired immune deficiency syndrome Comprehensive Response Information Management System. Youths aged 15 to 24 years who were newly diagnosed with HIV between 2012 and 2016 and were living in Hangzhou were included in the study. Comparisons between student and non-student youths were made for ART initiation within 30 days, 90 days, and 12 months of HIV diagnosis, and the viral suppression rate at 12 months of HIV diagnosis and at 12 months of ART initiation. Results: A total of 707 cases met inclusion criteria, 29.6% of which were students and 70.4% were non-student youths. The student group had a higher proportion of ART initiation compared with the non-student group within 30 days of diagnosis (45.5% vs. 37.0%, P = 0.044), and a slightly higher but not statistically significant proportion at 90 days (67.0% vs. 62.7%), and 12 months (83.7% vs. 78.5%) of HIV diagnosis. ART initiation within 30 days improved from <15% in 2012 to over 65% in 2016 in both groups, and ART initiation within 90 days improved from <30% in 2012 to >90% in 2016. A smaller proportion of students experienced viral suppression compared with the non-student group (9.6% vs. 17.1%, P = 0.011) at 12 months after HIV diagnosis, but the suppression rate was similar at 12 months of ART initiation (69.9% vs. 71.1%, P = 0.743). Conclusions: ART initiation in both student and non-student youths has significantly improved between 2012 and 2016. However, the viral suppression rate remained unacceptably low at 12 months of HIV diagnosis in both student and non-student groups. Specific intervention strategies must be taken to address this challenge. 展开更多
关键词 Human immunodeficiency virus ANTIRETROVIRAL therapy Viral suppression STUDENT Non-student YOUTHS
Nomograms based on HPV load for predicting survival in cervical squamous cell carcinoma: An observational study with a longterm follow-up 预览
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作者 Jing Zuo Ying Huang +4 位作者 Jusheng An Xi Yang Ning Li Manni Huang Lingying Wu 《中国癌症研究:英文版》 SCIE CAS CSCD 2019年第2期389-399,共11页
Objective: To investigate the prognostic value of pretreatment human papillomavirus(HPV) viral load for cervical cancer, and to develop nomograms based on HPV load and other clinicopathological factors for long-term s... Objective: To investigate the prognostic value of pretreatment human papillomavirus(HPV) viral load for cervical cancer, and to develop nomograms based on HPV load and other clinicopathological factors for long-term survival.Methods: We conducted a prospective study on cervical squamous cell carcinoma(SCC) patients diagnosed between January 2003 and December 2008. Cervical samples were tested for HPV viral load by the Hybrid Capture II(HCII) assay before treatment and 6 months after treatment. Clinical characteristics and follow-up information were also collected. A multivariable Cox proportional hazards model was used to adjust covariates in both the radical hysterectomy(RH) treatment group and concurrent chemoradiotherapy(CCRT) treatment group to identify relevant covariates, and then nomograms were constructed and used for internal validation.Results: A total of 520 SCC patients enrolled in this study with a median follow-up of 127 months, 360 patients received RH, whereas 160 patients received CCRT. The median HPV viral load in RH and CCRT groups was356.10 and 294.29, respectively. Tumor size was positively correlated with high pretreatment HPV load in both groups. In CCRT group, the advanced International Federation of Gynecology and Obstetrics(FIGO) stage and enlarged retroperitoneal lymph node status determined by computed tomography(LNSCT) were correlated with low HPV load group. Initial HPV viral load, FIGO stage and lymph node metastasis were prognostic factors for RH group, whereas HPV viral load, squamous cell carcinoma antigen(SCC-Ag) level and LNSCT were identified as prognostic factors for CCRT group. Nomograms incorporating these predictors for 10-year progression-free survival(PFS) were constructed [concordance index(C-index): 0.756, 0.749].Conclusions: A low pretreatment HPV viral load is an independent prognostic factor for poor prognosis of cervical SCC and is related to other clinicopathological factors. The survival nomogram based on HPV viral load could predict the long-term prognosis. 展开更多
关键词 Cervical SQUAMOUS cell carcinoma human PAPILLOMAVIRUS viral LOAD SURVIVAL NOMOGRAM
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失代偿期肝硬化合并胆总管结石患者行十二指肠镜治疗的安全和有效性分析
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作者 林岩 严俊 +2 位作者 张奇煜 朱晓亮 李汛 《中华消化内镜杂志》 CSCD 北大核心 2019年第6期416-421,共6页
目的评价失代偿期肝硬化合并胆总管结石患者行ERCP治疗的安全性和有效性。方法收集2012年12月至2016年12月在兰州大学第一医院ERCP诊疗技术培训中心经ERCP治疗的失代偿期肝硬化合并胆总管结石的患者79例(肝硬化组)、慢性病毒性肝炎患者9... 目的评价失代偿期肝硬化合并胆总管结石患者行ERCP治疗的安全性和有效性。方法收集2012年12月至2016年12月在兰州大学第一医院ERCP诊疗技术培训中心经ERCP治疗的失代偿期肝硬化合并胆总管结石的患者79例(肝硬化组)、慢性病毒性肝炎患者92例(慢性病毒性肝炎组)以及无肝病患者随机抽取114例(无肝病组),对比分析ERCP治疗操作情况、肝功能指标术后改善情况以及并发症情况。结果肝硬化组ERCP术前凝血酶原时间为(12.9±2.2) s,多于慢性病毒性肝炎组(12.1±1.9) s和无肝病组(11.7±1.4) s,差异有统计学意义(F=21.530,P<0.001)。术中操作肝硬化组所用时间(58.58±19.40) min,多于慢性病毒性肝炎组(52.53±16.74) min和无肝病组(49.81±14.82) min,差异有统计学意义(F=6.444,P=0.002)。术后各组均无穿孔、死亡的病例。肝硬化组中3例(3.8%)患者行EST后出现十二指肠乳头少量渗血,其中2例为Child-Pugh C级,1例为Child-Pugh B级;1例(1.27%)Child-Pugh C级患者出现食管下段曲张静脉渗血,但该项与其他术中、术后并发症同样,3组间差异均无统计学意义(P>0.05)。肝硬化组ERCP术前、术后肝功能指标谷草转氨酶(66.0比53.0 IU/L)、谷丙转氨酶(61.0比52.0 IU/L)、γ-谷氨酰转移酶(318.0比231.0 IU/L)、碱性磷酸酶(232.0比210.0 μmol/L)、总胆红素(65.7比56.3 μmol/L)比较,差异均有统计学意义(P<0.05),显示肝功能较术前明显改善。结论Child-Pugh A级、B级失代偿期肝硬化患者行ERCP治疗胆总管结石安全性和有效性良好,对于Child-Pugh C级患者,应尽量在改善肝功能、凝血功能情况后行ERCP。 展开更多
关键词 胆总管结石病 肝硬化 肝炎 病毒性 胰胆管造影术 内窥镜逆行
Viral and Bacterial Etiology of Acute Febrile Respiratory Syndrome among Patients in Qinghai, China 预览
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作者 LIU Gao Shan LI Hong +3 位作者 ZHAO Sheng Cang LU Rou Jian NIU Pei Hua TAN Wen Jie 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第6期438-445,共8页
Objective This study was conducted to investigate the viral and bacterial etiology and epidemiology of patients with acute febrile respiratory syndrome(AFRS) in Qinghai using a commercial routine multiplex-ligation-nu... Objective This study was conducted to investigate the viral and bacterial etiology and epidemiology of patients with acute febrile respiratory syndrome(AFRS) in Qinghai using a commercial routine multiplex-ligation-nucleic acid amplification test(NAT)-based assay. Methods A total of 445 nasopharyngeal swabs specimens from patients with AFRS were analyzed using the RespiFinderSmart22 kit(PathoFinder BV, Netherlands) and the LightCycler 480 real-time PCR system. Results Among the 225(225/445, 51%) positive specimens, 329 positive pathogens were detected, including 298(90.58%) viruses and 31(9%) bacteria. The most commonly detected pathogens were influenza virus(IFV;37.39%;123/329), adenovirus(AdV;17.02%;56/329), human coronaviruses(HCoVs;10.94%;36/329), rhinovirus/enterovirus(RV/EV;10.03%;33/329), parainfluenza viruses(PIVs;8.51%;28/329), and Mycoplasma pneumoniae(M. pneu;8.51%;28/329), respectively. Among the co-infected cases(17.53%;78/445), IFV/AdV and IFV/M. pneu were the most common co-infections. Most of the respiratory viruses were detected in summer and fall. Conclusion In our study, IFV-A was the most common respiratory pathogen among 22 detected pathogens, followed by AdV, HCoV, RV/EV, PIV, and M. pneu. Bacteria appeared less frequently than viruses, and co-infection was the most common phenomenon among viral pathogens. Pathogens were distributed among different age groups and respiratory viruses were generally active in July, September, and November. Enhanced surveillance and early detection can be useful in the diagnosis, treatment, and prevention of AFRS, as well as for guiding the development of appropriate public health strategies. 展开更多
关键词 Acute FEBRILE RESPIRATORY SYNDROME VIRAL and BACTERIAL ETIOLOGY Prevalence Seasonal distribution
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儿童肠道病毒性脑炎的临床特点及脑脊液病毒检测结果分析 预览
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作者 李永春 李小芹 张迎辉 《安徽医药》 CAS 2019年第7期1369-1372,共4页
目的探讨肠道病毒性脑炎(EVE)儿童的临床特点及脑脊液病毒检测结果。方法回顾性分析河南省儿童医院2015年1月至2017年12月收治的216例EVE病儿病例资料。结果临床特点:发热症状占69.44%,头痛占64.35%,呕吐占75.00%;6~8月发病占39.35%,9~1... 目的探讨肠道病毒性脑炎(EVE)儿童的临床特点及脑脊液病毒检测结果。方法回顾性分析河南省儿童医院2015年1月至2017年12月收治的216例EVE病儿病例资料。结果临床特点:发热症状占69.44%,头痛占64.35%,呕吐占75.00%;6~8月发病占39.35%,9~11月占31.48%;白细胞(WBC)总数>10×10^9/L占68.98%,C反应蛋白(CRP)升高占57.87%;脑电图轻中度异常检出率21.76%。脑脊液病毒检测:肠道病毒(EV)通用核酸检测阳性率55.56%,其中埃柯病毒30(ECHO30)型占79.17%;特异性Ig M抗体阳性95例;不同年龄段、不同发病时间段病儿脑脊液EV通用核酸阳性检出率、特异性Ig M抗体阳性检出率比较均差异有统计学意义(P均<0.05)。预后:均治愈出院。结论儿童EVE主要症状为发热、头痛及呕吐,好发于夏、秋季;脑脊液病毒检测以ECHO30为主且特异性Ig M抗体检出率较高,经综合治疗后病儿预后良好。 展开更多
关键词 脑炎 病毒性 肠道病毒属 儿童 临床特点 脑脊液 病毒抗体
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Preface
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作者 Zhi-Ming Zheng Ke Lan +1 位作者 Eric O. Freed Zheng-Li Shi 《中国病毒学:英文版》 CAS CSCD 2019年第2期117-118,共2页
Viral infections remain a global threat to world health in the twenty-first century. They are caused by both DNA and RNA viruses and can manifest as acute or chronic infections, in some cases causing epidemics or even... Viral infections remain a global threat to world health in the twenty-first century. They are caused by both DNA and RNA viruses and can manifest as acute or chronic infections, in some cases causing epidemics or even global pandemics.Persistent viral infections lead to host immunodeficiency and the development of ~12% of human cancers worldwide. 展开更多
关键词 VIRAL INFECTIONS twenty-first CENTURY CANCERS WORLDWIDE
高渗盐水雾化吸入治疗中重度毛细支气管炎36例疗效观察 预览
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作者 王雪松 潘家华 《安徽医药》 CAS 2019年第1期147-151,共5页
目的观察高渗盐水雾化吸入治疗不同程度毛细支气管炎的临床效果,及通过测定外周血白介素-4(IL-4)、γ-干扰素(IFN-γ)、调节性T细胞(Treg)水平的变化,探讨高渗盐水治疗毛细支气管炎可能的免疫调节作用。方法选取2014年10月至2015年4月... 目的观察高渗盐水雾化吸入治疗不同程度毛细支气管炎的临床效果,及通过测定外周血白介素-4(IL-4)、γ-干扰素(IFN-γ)、调节性T细胞(Treg)水平的变化,探讨高渗盐水治疗毛细支气管炎可能的免疫调节作用。方法选取2014年10月至2015年4月在安徽省立医院儿科住院诊断为毛细支气管炎患儿,根据毛细支气管炎危重度评分选取中度(A组)及重度(B组)各36例,同时选取健康体检婴幼儿12例(C组)作为健康组,A、B每组再按随机数字表分为三组,每组12例,分别给予高渗盐水(3%氯化钠)、布地奈德、异丙托溴铵雾化吸入治疗。治疗前及治疗后d4记录各患儿危重度评分,采血检测IL-4、IFN-γ、Treg,并与C组比较。结果A组高渗盐水雾化吸入组治疗后d4患儿临床危重度评分为(3.4±1.4)分,较治疗前(6.1±1.1)分明显下降,与其余两组比较差异有统计学意义(P<0.05);该组治疗后外周血IL-4水平为(18.45±1.84)μg/L、IFN-γ水平为(7.65±0.60)ng/L、Treg比例为(10.00±0.86)%较其余两组恢复更快(P<0.05);B组患儿各指标在治疗后有改善,但三组组间比较差异无统计学意义(P>0.05)。结论高渗盐水雾化吸入治疗中度毛细支气管炎临床症状改善更加明显,外周血IL-4、IFN-γ、Treg恢复更快,提示高渗盐水雾化治疗效果优于布地奈德及异丙托溴铵,具有一定的免疫调节作用;高渗盐水雾化吸入治疗重度毛细支气管炎患儿,与布地奈德及异丙托溴铵相比无明显优势。 展开更多
关键词 细支气管炎 病毒性 盐水 高渗 干扰素Γ 白细胞介素4 T淋巴细胞 调节性 儿童
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2014-2017年烟台市甲型流感HA基因变异及疫苗效果分析
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作者 刘娟 徐迎春 +4 位作者 孙振璐 高巧 董兆静 徐小雯 宫连凤 《中华预防医学杂志》 CAS CSCD 北大核心 2019年第8期840-842,共3页
本研究对2014年4月至2017年8月烟台市两家哨点医院流感样病例采集咽试子标本,分离流感病毒,血凝抑制实验分型并对甲型流感HA基因序列测定,进行系统发生学分析及主要氨基酸位点变异分析,Pepitope模型评估甲型流感疫苗保护效果。结果显示:... 本研究对2014年4月至2017年8月烟台市两家哨点医院流感样病例采集咽试子标本,分离流感病毒,血凝抑制实验分型并对甲型流感HA基因序列测定,进行系统发生学分析及主要氨基酸位点变异分析,Pepitope模型评估甲型流感疫苗保护效果。结果显示:仅H3N2甲型流感病毒抗原性发生变异;甲型H1N1pdm09流感毒株8株属于6B.2,14株属于6B.1基因亚型,H3N2流感毒12株属于3C.3a基因亚型,33株属于3C.2a基因亚型;甲型流感病毒HA蛋白多个抗原位点、潜在糖基化位点发生变异;2015-2016和2016-2017年监测季甲型H1N1pdm09流感病毒的疫苗保护效果分别为最佳匹配疫苗的77.29%和79.21%;2014-2015、2015-2016和2016-2017年流感监测季甲型H3N2流感病毒的疫苗保护效果分别为最佳匹配疫苗的-5.18%、16.97%和42.05%。表明2014-2017年烟台地区甲型流感病毒持续发生基因变异,H3N2流感疫苗保护性较差而甲型H1N1pdm09流感疫苗具有较好的保护作用。 展开更多
关键词 流感病毒A型 血凝素 病毒 抗原变异 疫苗效果 监测
超敏C-反应蛋白在新生儿感染中的应用
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作者 牛军小 《智慧健康》 2019年第16期3-4,共2页
目的研究新生儿细菌感染和病毒感染体内血清中超敏C-反应蛋白含量的差别,其对于临床区分细菌和病毒感染的效果及价值。方法选取我院2017年11月至2018年11月已确定的患肺炎患者50例新生儿作为本次探究对象,分为细菌感染组30例新生儿,病... 目的研究新生儿细菌感染和病毒感染体内血清中超敏C-反应蛋白含量的差别,其对于临床区分细菌和病毒感染的效果及价值。方法选取我院2017年11月至2018年11月已确定的患肺炎患者50例新生儿作为本次探究对象,分为细菌感染组30例新生儿,病毒感染组20例新生儿。对两组新生儿进行超敏C-反应蛋白测定,并进行统计分析。结果观察数据显示,细菌感染组超敏C-反应蛋白含量大于5mg/L的占93.33%,表明细菌感染的新生儿超敏C-反应蛋白含量明显升高,病毒感染组结果超敏C-反应蛋白含量大于5mg/L的占10.00%,表明病毒感染的新生儿血清超敏C-反应蛋白含量趋于正常。结论对新生儿感染应用超敏C-反应蛋白测定,对于临床医生区分新生儿细菌和病毒感染具有一定的帮助作用。 展开更多
关键词 超敏C-反应蛋白 新生儿感染 细菌 病毒 应用效果
HBV-DNA实时荧光定量检测程序的测量不确定度评估及分析 预览 被引量:1
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作者 刘伟平 杨新春 殷明刚 《国际检验医学杂志》 CAS 2019年第1期59-61,共3页
目的根据国际标准化组织(ISO)15189实验室认可要求,评估乙型肝炎病毒(HBV)DNA定量检测程序的测量不确定度。方法采用室内质控获得的中间精密度计算HBV-DNA测量重复性引入的测量不确定度,采用卫生部室间质量评价结果计算HBV-DNA偏倚引入... 目的根据国际标准化组织(ISO)15189实验室认可要求,评估乙型肝炎病毒(HBV)DNA定量检测程序的测量不确定度。方法采用室内质控获得的中间精密度计算HBV-DNA测量重复性引入的测量不确定度,采用卫生部室间质量评价结果计算HBV-DNA偏倚引入的测量不确定度,将二者合成计算出合成标准不确定度和扩展不确定度。结果HBV-DNA在室内质控水平为4.03(对数的倒数)时实验室内测量重复性引入的相对测量不确定度为3.45%,HBV-DNA偏倚引入的相对测量不确定度为5.27%。相对合成标准不确定度为6.30%;相对扩展不确定度为12.60%;而HBV-DNA在该水平的相对测量不确定度可表示为(4.03±12.60)%。结论HBV-DNA实时荧光定量检测程序的测量不确定度可作为检验结果质量持续改进的依据,并用于解释临床结果。 展开更多
关键词 肝炎病毒 乙型 DNA 病毒 聚合酶链反应 荧光
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补充性肠外营养治疗对重症病毒性脑炎患儿免疫水平及医院感染的影响 预览
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作者 陈锋 张芙蓉 +1 位作者 陈洋 许瑜 《重庆医学》 CAS 2019年第15期2554-2557,共4页
目的比较肠内营养(EN)与补充性肠外营养(SPN)治疗对重症病毒性脑炎(SVE)患儿免疫水平及医院感染的影响。方法回顾性分析2012年1月至2017年12月该院重症医学科收治的126例SVE患儿临床资料,根据营养途径分为SPN组(n=59)与EN组(n=67),SPN... 目的比较肠内营养(EN)与补充性肠外营养(SPN)治疗对重症病毒性脑炎(SVE)患儿免疫水平及医院感染的影响。方法回顾性分析2012年1月至2017年12月该院重症医学科收治的126例SVE患儿临床资料,根据营养途径分为SPN组(n=59)与EN组(n=67),SPN组采用EN联合SPN进行营养支持,EN组采取留置胃管的方式进行EN支持,比较两组患者的营养状况、免疫水平和医院感染状况。结果营养干预后第14天SPN组血清总蛋白(TP)水平,干预后第7、14天SPV组转铁蛋白(TRF)水平较EN组升高,差异均有统计学意义(t=3.12、2.08、2.29,P<0.05)。干预后第14天,两组患儿外周血CD3^+、CD4^+、CD19^+细胞百分比、CD4^+/CD8^+比值及IgG、IgA、IgM水平均较干预前增加,差异有统计学意义(P<0.05)。营养干预后,SPN组CD4^+、CD19^+细胞百分比高于EN组,差异均有统计学意义(P<0.05)。SPN组患者机械通气时间、儿科重症监护室(PICU)住院时间低于EN组,差异均有统计学意义(P<0.01)。EN组医院感染率(17.91%)低于SPN组(33.90%),差异有统计学意义(P<0.05)。结论SPN治疗可以促进SVE患儿的免疫功能恢复,提高临床疗效,但较EN增加了患儿发生医院感染的风险。 展开更多
关键词 补充性肠外营养 脑炎 病毒性 免疫功能 医院感染
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Recent Progress on Functional Genomics Research of Enterovirus 71
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作者 Huiqiang Wang Yuhuan Li 《中国病毒学:英文版》 CAS CSCD 2019年第1期9-21,共13页
Enterovirus 71(EV71) is one of the main pathogens that causes hand-foot-and-mouth disease(HFMD). HFMD caused by EV71 infection is mostly self-limited;however, some infections can cause severe neurological diseases, su... Enterovirus 71(EV71) is one of the main pathogens that causes hand-foot-and-mouth disease(HFMD). HFMD caused by EV71 infection is mostly self-limited;however, some infections can cause severe neurological diseases, such as aseptic meningitis, brain stem encephalitis, and even death. There are still no effective clinical drugs used for the prevention and treatment of HFMD. Studying EV71 protein function is essential for elucidating the EV71 replication process and developing anti-EV71 drugs and vaccines. In this review, we summarized the recent progress in the studies of EV71 noncoding regions(50 UTR and 30 UTR) and all structural and nonstructural proteins, especially the key motifs involving in viral infection, replication, and immune regulation. This review will promote our understanding of EV71 virus replication and pathogenesis, and will facilitate the development of novel drugs or vaccines to treat EV71. 展开更多
关键词 ENTEROVIRUS 71 (EV71) FUNCTIONAL GENOMICS Structural PROTEIN Nonstructural PROTEIN VIRAL PROTEIN function
Correlation between SP-D content in cerebrospinal fluid and severity of viral encephalitis
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作者 Chuang Zhang Xi Yang Rong Jiao 《海南医科大学学报(英文版)》 2019年第1期45-49,共5页
Objective:To detect the content of SP-D in cerebrospinal fluid(CSF) of children with viral encephalitis, evaluate the correlation between SP-D and indexes of brain injury, inflammatory reaction and cellular immune dis... Objective:To detect the content of SP-D in cerebrospinal fluid(CSF) of children with viral encephalitis, evaluate the correlation between SP-D and indexes of brain injury, inflammatory reaction and cellular immune disorder.Methods: 78 cases of viral encephalitis children diagnosed in our hospital during December 2016 to May 2018 were selected as Viral encephalitis group, 51 cases of fever without intracranial infection children were selected as Control group. The cerebrospinal fluid samples of two groups were taken and SP-D, brain injury-related indicators, inflammatory indicators and T lymphocyte subsets were detected. Pearson test was used to analyze the relationship between SP-D content in CSF and above indicators.Results: Content of SP-D in CSF of Viral encephalitis group was lower than that of control group (P<0.05). Contents of MBP, CK-BB, NSE and S100B in CSF of Viral encephalitis group were higher than those of Control group;contents of IL-1β, TNF-αand ICAM-1 in CSF of Viral encephalitis group were higher than those of Control group;distribution proportion of CD3+, CD4+ T lymphocytes and CD4+/CD8+ ratio in CSF were lower than those of Control group, and levels of CD8+ were higher than that of Control group (P<0.05). Correlation analysis showed that, SP-D content in CSF of viral encephalitis children was directly related to brain injury indexes, inflammatory indexes and T lymphocyte subsets distribution levels(P<0.05).Conclusion: Content of SP-D in CSF of viral encephalitis children decreases abnormally, the specific content is directly related to the degree of brain injury, intracranial inflammation and cellular immune dysfunction. It is important to evaluate the severity of the disease. 展开更多
关键词 VIRAL ENCEPHALITIS SP-D Brain damage INFLAMMATORY reaction T LYMPHOCYTE
Characteristics and advantages of adenoassociated virus vector-mediated gene therapy for neurodegenerative diseases 预览
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作者 Yuan Qu Yi Liu +2 位作者 Ahmed Fayyaz Noor Johnathan Tran Rui Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期931-938,共8页
Common neurodegenerative diseases of the central nervous system are characterized by progressive damage to the function of neurons,even leading to the permanent loss of function.Gene therapy via gene replacement or ge... Common neurodegenerative diseases of the central nervous system are characterized by progressive damage to the function of neurons,even leading to the permanent loss of function.Gene therapy via gene replacement or gene correction provides the potential for transformative therapies to delay or possibly stop further progression of the neurodegenerative disease in affected patients.Adeno-associated virus has been the vector of choice in recent clinical trials of therapies for neurodegenerative diseases due to its safety and efficiency in mediating gene transfer to the central nervous system.This review aims to discuss and summarize the progress and clinical applications of adeno-associated virus in neurodegenerative disease in central nervous system.Results from some clinical trials and successful cases of central neurodegenerative diseases deserve further study and exploration. 展开更多
关键词 nerve REGENERATION central nervous system gene therapy NEURODEGENERATIVE DISEASE viral vector ADENO-ASSOCIATED virus Alzheimer’s DISEASE Parkinson’s DISEASE Huntington’s DISEASE amyotrophic lateral SCLEROSIS spinal muscular atrophy neural REGENERATION
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上海市2014-2017年5岁以下住院儿童病毒性腹泻病原学特征分析
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作者 匡小舟 肖文佳 +4 位作者 滕峥 潘浩 袁政安 吴凡 张曦 《中华流行病学杂志》 CAS CSCD 北大核心 2019年第8期895-899,共5页
目的了解目标人群中病毒病原谱及其分子流行病学特征,为病毒性腹泻的防控、用药和疫苗的研发及使用提供科学依据。方法采集2014-2017年上海市某儿科医院<5岁腹泻住院患儿的粪便标本,并收集患儿的人口学、临床和流行病学信息。采用EL... 目的了解目标人群中病毒病原谱及其分子流行病学特征,为病毒性腹泻的防控、用药和疫苗的研发及使用提供科学依据。方法采集2014-2017年上海市某儿科医院<5岁腹泻住院患儿的粪便标本,并收集患儿的人口学、临床和流行病学信息。采用ELISA、荧光PCR以及巢式PCR方法对轮状病毒、杯状病毒、星状病毒和肠道腺病毒进行核酸检测和分子分型。结果共采集粪便标本1018份(男性患儿671份,女性患儿347份),病毒阳性检出率高达40.57%,每年秋、冬季节达到检出高峰,检出率较高为杯状病毒(24.75%)和A组轮状病毒(13.95%)。在0~6月龄的婴幼儿中病毒检出率最低(32.20%)。约有65%的病毒感染患儿曾使用抗生素治疗,且抗生素的入院前使用率在病毒感染和病毒阴性人群中差异无统计学意义(P>0.05)。G9P[8]基因型为轮状病毒的主导流行株,且轮状病毒在<5岁各年龄组中普遍易感。结论上海市<5岁腹泻住院患儿中杯状病毒的阳性检出率高于轮状病毒,提示需要密切关注婴幼儿腹泻的病原谱及病毒型别的变迁,在治疗中应合理使用抗生素,并加强疫苗研发用于疾病防控。 展开更多
关键词 婴幼儿 住院 病毒 急性腹泻
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