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Impact of Luveris and Gonal-F on bovine immature oocytes developmental competence in vitro
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作者 Xue-Mei Wang Yan-Jing Guo +6 位作者 Rui-Ge Gao Jing Li Xin-Yuan Liu Zhe Wu You-You Li Li-Ping Yang Cai-Hong Chang 《海南医科大学学报(英文版)》 2019年第3期1-4,共4页
Objective: To investigate the gonadotrophin drug Luveris? (L) and Gonal-F? (G) effects on bovine immature oocyte in vitro maturation culture. Methods: In total, 2000 bovine cumulus-oocyte complexes (COCs) were purchas... Objective: To investigate the gonadotrophin drug Luveris? (L) and Gonal-F? (G) effects on bovine immature oocyte in vitro maturation culture. Methods: In total, 2000 bovine cumulus-oocyte complexes (COCs) were purchased commercially and cultured in media with ovulation stimulation drugs Gonal-F and Luveris (0, 5, 10, 20, 40 IU/mL), individually, for maturation in vitro. 2000 Oocytes were divided evenly into two groups, Luveris and Gonal-F groups, individually, 1000 Oocytes. After 24 and 48h culture, cumulus expansion was assessed under dissecting microscopes. Oocyte maturation (GV, MI, MII) was determined by examining nuclear maturation and polar body formation under inverted microscopes. Results: Luveris and Gonal-F enhanced oocyte maturation in vitro in a dose-dependently manner. Culture media supplemented with L or G significantly improved MⅡ Oocyte maturation rates after culture for 24 h and 48 h in comparison with the control group. Oocyte maturation rates were 33.3% and 37.5%(20 IU/mL), individually, 33.3% and 53.6% (40 IU/mL);20 IU/mL G was 15.1% and 16.2%;40 IU/mL G was 38.9% and 39.5%, respectively. Conclusion: LH (Luveris) can promote prophase bovine immature oocytes developmental competence in vitro, which is mostly likely stimulated by FSH(Gonal-F ). Furthermore, LH can delay oocytes GVBD that is very useful for IVF clinical medication guidance. 展开更多
关键词 LH Luveris Gonal-F OOCYTES DEVELOPMENTAL Competence MATURATION in VITRO Culture in VITRO
Effects of Electroacupuncture on Expression of PI3K/Akt/Foxo3a in Granulosa Cells from Women with Shen(Kidney) Deficiency Syndrome Undergoing in vitro Fertilization-Embryo Transfer
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作者 WU Hai-cui ZHANG Jian-wei +3 位作者 SUN Zhen-gao XIANG Shan QIAO Yan LIAN Fang 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第4期252-258,共7页
Objective: To observe the effects of electroacupuncture(EA) on reproductive outcomes in women with Shen(Kidndy) deficiency syndrome after in vitro fertilization-embryo transfer(IVF-ET),and explore the underlying molec... Objective: To observe the effects of electroacupuncture(EA) on reproductive outcomes in women with Shen(Kidndy) deficiency syndrome after in vitro fertilization-embryo transfer(IVF-ET),and explore the underlying molecular mechanism.Methods: Sixty-six infertile patients with Shen de?ciency syndrome undergoing IVF-ET were divided into EA or control groups according to a random table,33 cases in each group.Before undergoing IVF,patients in the EA and control groups received EA therapy and placebo needle puncture,respectively,for 3 menstrual cycles.Shen de?ciency syndrome scores were assessed.Other outcome measures included the number of retrieved oocytes and fertilization,high-quality embryo and clinical pregnancy rates.Follicular ?uid was collected on the day of oocyte retrieval,and granulosa cell expression of phosphatidylinositide3-kinases(PI3 K),serine-threonine kinase(Akt) and forkhead box O3(Foxo3 a) m RNA were measured by reverse transcribed and quantitative real-time polymerase chain reaction.Results: Syndrome scores for pre-versus post-treatments decreased significantly(16.53±1.75 to 8.67±1.61) in the EA group(P<0.05),but showed no signi?cant change in the control group(17.18±1.58 to 14.74±1.58).A signi?cant difference in score change was found between the EA and control groups(P<0.05).High-quality embryo and clinical pregnancy rates were both increased in the EA group compared with the control group [69.15%(195/282) vs.60.27%(176/292) and 66.67%(22/33) vs.42.42%(14/33),respectively,P<0.05].The fertilization rate was equivalent in EA and control groups.No difference was found in the number of retrieved oocytes between the two groups.Granulosa cell expression levels of PI3 K and Akt m RNA were signi?cantly increased in the EA group compared with the control group,while the expression of Foxo3 a was reduced(all P<0.05).Conclusions: For infertile patients with Shen de?ciency syndrome undergoing IVF,EA for tonifying Shen as an adjunct treatment may alleviate clinical symptoms and improve the high-quality em 展开更多
关键词 in vitro fertilization-embryo transfer SHEN deficiency ELECTROACUPUNCTURE GRANULOSA cells PI3K/Akt/Foxo3a
In Vitro Probiotic Effect of Pseudostellaria heterophylla Fibrous Roots on Feed Bacillus subtilis and Preliminary Study on Its Solid Fermentation 预览
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作者 Pan Huiqing Zhang Yanda +1 位作者 Li Zhujin Zhao Qi 《动物与饲料科学:英文版》 CAS 2019年第2期72-76,共5页
Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla f... Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla fibrous roots extract on feed probiotics Bacillus subtilis BD-K010 was studied, and the feasibility of solid fermentation of P. heterophylla fibrous roots was preliminarily evaluated. For in vitro probiotic effect, the increased concentration of P. heterophylla fibrous roots extract dependently increased the total biomass of B. subtilis BD-K010;1.0% P. heterophylla fibrous roots extract received the best effect, and the final p H of 1.0% experimental group was closer to neutral. Meantime, B. subtilis BD-K010 with optimum concentration of P. heterophylla fibrous roots showed significantly higher in vitro antibacterial effect than the control group(P<0.01),and the antibacterial effects on Escherichia coli and Staphylococcus aureus were improved by 51.99% and 63.16%, but it was ineffective to Salmonella. For solid fermentation, the profile of substrate complex and appendage flocculent structure on substrate surface at the end of fermenta-tion in experimental groups added with B. subtilis BD-K010 and cellulase plus BD-K010 were more complex;the live bacteria number, polysaccharide content and saponin content at fermentation end-point in two experimental groups were extremely higher than those in the control group(P<0.01).P. heterophylla fibrous roots extract had good in vitro probiotic effect on B. subtilis BD-K010 and promoted its antibacterial effect, and it is feasible to use probiotics for solid fermentation of P. heterophylla fibrous roots and to improve effective components. It is of great significance to further de-velop and utilize P. heterophylla fibrous roots resources in modern animal husbandry. 展开更多
关键词 Pseudostellaria heterophylla FIBROUS roots FEED PROBIOTICS Bacillus SUBTILIS In VITRO probiotic EFFECT Solid fermentation
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Successful treatment with hysteroscopy for infertility due to isthmocele and hydrometra secondary to cesarean section: A case report 预览
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作者 Luis Pablo López Rivero Miguel Jaimes +1 位作者 Felipe Camargo Esther López-Bayghen 《世界临床病例杂志》 2019年第6期753-758,共6页
BACKGROUND An isthmocele is a scar defect in the uterine wall due to a cesarean section. Its prevalence is unknown, but it has been associated with secondary infertility. Here, we present a case where the patient suff... BACKGROUND An isthmocele is a scar defect in the uterine wall due to a cesarean section. Its prevalence is unknown, but it has been associated with secondary infertility. Here, we present a case where the patient suffered from an isthmocele that was associated with persistent hydrometra, which developed during in vitro fertilization. CASE SUMMARY The patient underwent hysteroscopic surgery, which successfully resolved the isthmocele as well as the hydrometra. Afterward, two high-quality, euploid embryos, determined by morphological assessment and pre-implantation genetic diagnostic testing, were transferred. This resulted in uterine pregnancy, as determined byserum β-human chorionic gonadotropin levels on day 14 (180 mU/mL) and ultrasound-confirmed presence of a gestational sac with a positive embryocardia at week 6. The pregnancy reached 36 wk without any complications, and the product was born in good health. We report a successful isthmocele treatment in a patient with secondary infertility, in which the isthmocele was the cause of persistent hydrometra. CONCLUSION Hydrometra caused by secondary cesarean is an infertility factor, which can be corrected by hysteroscopy plus ablation of the isthmocele. 展开更多
关键词 In VITRO FERTILIZATION Isthmocele Hydrometra Case REPORT
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Progress in in vitro culture and gene editing of porcine spermatogonial stem cells
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作者 Yi-Zhuo Sun Si-Tong Liu +1 位作者 Xiao-Meng Li Kang Zou 《动物学研究》 CAS CSCD 2019年第5期343-348,共6页
Research on in vitro culture and gene editing of domestic spermatogonial stem cells (SSCs) is of considerable interest but remains a challenging issue in animal science. In recent years, some progress on the isolation... Research on in vitro culture and gene editing of domestic spermatogonial stem cells (SSCs) is of considerable interest but remains a challenging issue in animal science. In recent years, some progress on the isolation, purification, and genetic manipulation of porcine SSCs has been reported. Here, we summarize the characteristics of porcine SSCs as well current advances in their in vitro culture, potential usage, and genetic manipulation. Furthermore, we discuss the current application of gene editing in pig cloning technology. Collectively, this commentary aims to summarize the progress made and obstacles encountered in porcine SSC research to better serve animal husbandry, improve livestock fecundity, and enhance potential clinical use. 展开更多
关键词 PROGRESS VITRO CULTURE STEM cells
Preservation of Juniperus thurifera L.:a rare endangered species in Algeria through in vitro regeneration 预览
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作者 Nadia Khater Halima Benbouza 《林业研究:英文版》 CAS CSCD 2019年第1期77-86,共10页
Juniperus thurifera L.is an endemic Cupressaceae from the Aures Mountains of north eastern Algeria and endangered,in part,due to the scarcity of viable seeds.It is threatened by other abiotic factors and the lack of a... Juniperus thurifera L.is an endemic Cupressaceae from the Aures Mountains of north eastern Algeria and endangered,in part,due to the scarcity of viable seeds.It is threatened by other abiotic factors and the lack of an effective management strategy will increase its risk of extinction.The dearth of information on its in vitro regeneration impedes its application in forest management programs.We therefore developed a micropropagation protocol using microcuttings with auxiliary buds.Cuttings were grown on different combinations of media supplemented with plant growth regulators at different concentrations.The highest number of shoots and branches regenerated from original shoots was obtained on Woody Plant Medium(WPM)supplemented with 6-benzylaminopurine(BAP)(0.5 mg L^-1)and 2,4-dichlorophenoxyacetic acid(2,4-D)(0.25 mg L^-1).The best elongation of shoots was achieved with WPM supplemented with 0.5 mg L^-1 of BAP and 0.25 or 1 mg L^-1 of 2,4-D.On the second subculture,shoots had a higher number of branches than those of the first.The highest rooting rate,38.8%,was obtained with shoots cultured in 1/2 Murashige and Skoog(MS)medium supplemented with 5.0 mg L^-1 each of indol-3-butyric(IBA)and naphthalene acetic acid(NAA).Similarly,the highest root numbers and lengths were produced on 1/2 MS medium supplemented with IBA and NAA(5.0 mg L^-1 each).During transfer to acclimatization,rates of plant losses of 50%occurred.The second part of the experiment showed that the best shoot callusing was on WPM supplemented with BAP and 2,4-D,with either the combination 0.5+0.25 or 0.25+0.25 mg L^-1.The results of this research provide a starting point for further studies on in vitro regeneration of J.thurifera for the sustainable management of its unique ecosystem in the Mediterranean basin. 展开更多
关键词 JUNIPERUS thurifera CALLUS CONSERVATION REGENERATION In VITRO culture
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Secondary sex ratio of assisted reproductive technology babies
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作者 Nathira Abdul Majeed Charley Zheng +3 位作者 Alex Polyakov Megan Pucci Mohamed Hatta Tarmizi Mie Mie Cho Win 《亚太生殖杂志(英文版)》 2019年第2期88-92,共5页
Objective: To assess the secondary sex ratio (SSR) of assisted reproduction technology babies within a fertility clinic in Melbourne, Australia. Methods: A retrospective cohort study was conducted on 3369 babies who w... Objective: To assess the secondary sex ratio (SSR) of assisted reproduction technology babies within a fertility clinic in Melbourne, Australia. Methods: A retrospective cohort study was conducted on 3369 babies who were born following single embryo transfer between 2011 and 2016. Variables examined included embryo creation date, maternal and paternal ages, maternal body mass index (BMI), type of infertility, sperm parameters, fertilization method, type of embryo and stage of transfer. Multivariate regression analysis was performed on the data set using STRATA V9.2. Results: More males were found to be born to embryos created in summer (adjusted odds ratio=1.46, 95% confidence interval: 1.08-1.95;P=0.01). Lower BMI of mother (≤30 kg/m 2) and younger age of parents (≤35 years) were associated with an increase in SSR (50.1% vs. 47.1%;50.6% vs. 48.8%;50.0% vs. 48.9%) respectively. Decreased SSR value was associated with primary subfertility, using in-vitro fertilization procedure and fresh embryo compared to those with secondary subfertility, using intracytoplasmic sperm injection procedure and thawed embryo (49.3% vs. 50.6%;47.1% vs. 50.8%;49.4% vs. 50.1%) respectively. Conclusions: The fertilization methods, type of embryo, stage of embryo transfer, parameters of the sperm and status of subfertility do not significantly affect the SSR. SSR is affected by the seasonality, maternal age, and BMI. 展开更多
关键词 Artificial REPRODUCTION technology INTRACYTOPLASMIC SPERM injection In-vitro FERTILIZATION SECONDARY SEX ratio
Characteristics of β-oxidative and reductive metabolism on the acyl side chain of cinnamic acid and its analogues in rats
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作者 Kai-jing Zhao Yang Chen +11 位作者 Shi-jin Hong Yi-ting Yang Jiong Xu Han-yu Yang Liang Zhu Ming Liu Qiu-shi Xie Xian-ge Tang Ting-ting Yang Ya-qian Zhou Li Liu Xiao-dong Liu 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第8期1106-1118,共13页
Cinnamic acid and its analogues (pyragrel and ozagrel) undergo chain-shortened (β-oxidative) and reductive metabolism on acyl side chain. In this study, we characterized the β-oxidative and reductive metabolism on a... Cinnamic acid and its analogues (pyragrel and ozagrel) undergo chain-shortened (β-oxidative) and reductive metabolism on acyl side chain. In this study, we characterized the β-oxidative and reductive metabolism on acyl side chain of cinnamic acid and its analogues using primary rat hepatocytes, hepatic mitochondrial, and microsomal systems. A compartmental model including parent compounds and metabolites was developed to characterize in vivo β-oxidative and reductive metabolism following an intravenous dose of parent compounds to rats. The ?tted total in vivo clearance values were further compared with the in vitro values predicted by the well-stirred model. We showed that hepatic microsomal CYP450s did not catalyze β-oxidative or reductive metabolism of the three compounds. Similar to β-oxidation of fatty acids,β-oxidative metabolism on their acyl side chain occurred mainly in mitochondria, which was highly dependent on ATP, CoA and NAD+. Fatty acids and NADH inhibited the β-oxidative metabolism. Reductive metabolism occurred in both mitochondria and microsomes. Reduction in mitochondria was ATP-, CoA-, and NAD(P)H-dependent and reversible, which was suppressed by enoyl reductase inhibitor triclosan. Reduction in microsomes was ATP-, CoA-, and NADPH-dependent but little affected by triclosan. Both plasma concentrations of β-oxidative metabolites and reductive metabolites were successfully ?tted using the compartmental model. The estimated total in vivo clearance values were consistent with those predicted from hepatocytes and organelles, implicating signi?cance of in vitro kinetics. These ?ndings demonstrate the roles of hepatic mitochondria and microsomes in β-oxidative and reductive metabolism on acyl side chain of cinnamic acid and its analogues along with their metabolic characteristics. 展开更多
关键词 ANTIPLATELET DRUG cinnamic acid pyragrel OZAGREL DRUG METABOLISM hepatocytes mitochondria MICROSOMES pharmacokinetics in vitro-in vivo correlation
Application of custom anatomy-based nerve conduits on rabbit sciatic nerve defects: in vitro and in vivo evaluations 预览
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作者 Yamuhanmode·Alike Maimaiaili·Yushan +6 位作者 Ajimu·Keremu Alimujiang·Abulaiti Zhen-Hui Liu Wei Fu Li-Wei Yan Aihemaitijiang·Yusufu Qing-Tang Zhu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2173-2182,共10页
The intermingling of regenerated nerve fibers inside nerve grafts is the main reason for mismatched nerve fibers. This is one of the key factors affecting limb function recovery after nerve injury. Previous research h... The intermingling of regenerated nerve fibers inside nerve grafts is the main reason for mismatched nerve fibers. This is one of the key factors affecting limb function recovery after nerve injury. Previous research has shown that the accuracy of axon regeneration can be improved by a bionic structural implant. To this aim, iodine and freeze-drying high-resolution micro-computed tomography was performed to visualize the 3D topography of the New Zealand rabbit sciatic nerve (25 mm). A series of 1-, 2-, 3-, and 4-custom anatomy-based nerve conduits (CANCs) were fabricated based on the anatomical structure of the nerve fascicle. The match index, luminal surface, and mechanical properties of CANCs were evaluated before implanting in a 10-mm gap of the sciatic nerve. Recovery was evaluated by histomorphometric analyses, electrophysiological study, gastrocnemius muscle weight recovery ratio, and behavioral assessments at 12 and 24 weeks postoperatively. The accuracy of nerve regeneration was determined by changes in fluorescence-labeled profile number during simultaneous retrograde tracing. Our results showed that the optimal preprocessing condition for high-resolution micro-computed tomography visualization was treatment of the sciatic nerve with 40% Lugol’s solution for 3 days followed by lyophilization for 2 days. In vitro experiments demonstrated that the match index was highest in the 3-CANC group, followed by the 2-, 1-, and 4-CANC groups. The luminal surface was lowest in the 1-CANC group. Mechanical properties (transverse compressive and bending properties) were higher in the 3- and 4-CANC groups than in the 1-CANC group. In vivo experiments demonstrated that the recovery (morphology of regenerated fibers, compound muscle action potential, gastrocnemius muscle weight recovery ratio, pain-related autotomy behaviors, and range of motion) in the 3-CANC group was superior to the other CANC groups, and achieved the same therapeutic effect as the autograft. The simultaneous retrograde tracing results showed that th 展开更多
关键词 NERVE REGENERATION NERVE conduits mismatch iodine and FREEZE-DRYING high-resolution micro-computed tomography bio-mimic CUSTOM RABBIT SCIATIC NERVE in vitro in vivo neural REGENERATION
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Association between TSH Level and Pregnancy Outcomes in Euthyroid Women Undergoing IVF/ICSI:A Retrospective Study and Meta analysis 预览
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作者 Lei JIN Meng WANG +2 位作者 Jing YUE Gui-jin ZHU Bo ZHANG 《当代医学科学(英文)》 SCIE CAS 2019年第4期631-637,共7页
The aim of this study was to determine the association between thyroid-stimulating hormone (TSH) level and pregnancy outcomes in euthyroid women undergoing in vitro fertilization (IVF)/intra-cytoplasmic sperm injectio... The aim of this study was to determine the association between thyroid-stimulating hormone (TSH) level and pregnancy outcomes in euthyroid women undergoing in vitro fertilization (IVF)/intra-cytoplasmic sperm injection (ICSI).A total of 1185 women were enrolled in the retrospective study,and 12 studies with a total of 6624 women were included in the meta-analysis (including the data of the present retrospective study).Participants in the retrospective study were divided into two groups in terms of their serum TSH levels:TSH <2.5 mIU/L group (w=830) and TSH >2.5 mIU/L group (h=355).They were monitored for the status of clinical pregnancy or miscarriage.In the TSH <2.5 mIU/L group,441 (53.1%) women achieved clinical pregnancy,while 48 (5.8%) had early pregnancy loss and 12 (1.4%) had ectopic pregnancy.In the TSH >2.5 mIU/ L group,175 (49.3%) women achieved clinical pregnancy,while 21 (5.9%) had early pregnancy loss and 3 (0.8%) had ectopic pregnancy.No significant differences were observed between the two groups in pregnancy outcomes (P=0.126,P=0.512,P=0.297).The meta-analysis also revealed no significant difference in the clinical pregnancy rate and the miscarriage rate between women with serum TSH <2.5 mIU/L and those with serum TSH >2.5 mIU/L.In conclusion,high TSH levels (TSH level >2.5 mIU/L) did not affect clinical pregnancy rate or increase miscarriage rate in euthyroid women undergoing IVF/ICSI. 展开更多
关键词 euthyroid PREGNANCY outcomes in VITRO FERTILIZATION intra-cytoplasmic SPERM injection
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Suitability and limitations of mesenchymal stem cells to elucidate human bone illness 预览
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作者 Izaskun Mitxitorena Arantza Infante +1 位作者 Blanca Gener Clara I Rodríguez 《世界干细胞杂志:英文版(电子版)》 2019年第9期578-593,共16页
Functional impairment of mesenchymal stem cells(MSCs),osteoblast progenitor cells,has been proposed to be a pathological mechanism contributing to bone disorders,such as osteoporosis(the most common bone disease)and o... Functional impairment of mesenchymal stem cells(MSCs),osteoblast progenitor cells,has been proposed to be a pathological mechanism contributing to bone disorders,such as osteoporosis(the most common bone disease)and other rare inherited skeletal dysplasias.Pathological bone loss can be caused not only by an enhanced bone resorption activity but also by hampered osteogenic differentiation of MSCs.The majority of the current treatment options counteract bone loss,and therefore bone fragility by blocking bone resorption.These socalled antiresorptive treatments,in spite of being effective at reducing fracture risk,cannot be administered for extended periods due to security concerns.Therefore,there is a real need to develop osteoanabolic therapies to promote bone formation.Human MSCs emerge as a suitable tool to study the etiology of bone disorders at the cellular level as well as to be used for cell therapy purposes for bone diseases.This review will focus on the most relevant findings using human MSCs as an in vitro cell model to unravel pathological bone mechanisms and the application and outcomes of human MSCs in cell therapy clinical trials for bone disease. 展开更多
关键词 Mesenchymal stem cells Bone illness Osteoporosis OSTEOGENESIS Osteoanabolic THERAPIES In VITRO CELL models CELL therapy
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Trabecular-like Ti-6Al-4V scaffolds for orthopedic: fabrication by selective laser melting and in vitro biocompatibility
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作者 Huixin Liang Youwen Yang +6 位作者 Deqiao Xie Lan Li Ning Mao Changjiang Wang Zongjun Tian Qing jiang Lida Shen 《材料科学技术学报:英文版》 SCIE EI CAS CSCD 2019年第7期1284-1297,共14页
Porous metal scaffolds play an important role in the orthopedic field, due to their wide applications in prostheses implantation. Some previous studies showed that the scaffolds with trabecular bone structure reconstr... Porous metal scaffolds play an important role in the orthopedic field, due to their wide applications in prostheses implantation. Some previous studies showed that the scaffolds with trabecular bone structure reconstructed via computed tomography had satisfactory biocompatibility. However, the reverse modeling scaffolds were inflexible for customized design. Therefore, a top-down designing biomimetic bone scaffold with favorable mechanical performances and cytocompatibility is urgently demanded for orthopedic implants. An emerging additive manufacturing technique, selective laser melting, was employed to fabricate the trabecular-like porous Ti-6Al-4 V scaffolds with varying irregularities(0.05-0.5) and porosities(48.83%–74.28%) designed through a novel Voronoi-Tessellation based method. Micro-computed tomography and scanning electron microscopy were used to characterize the scaffolds’ morphology.Quasi-static compression tests were performed to evaluate the scaffolds’ mechanical properties. The MG63 cells culture in vitro experiments, including adhesion, proliferation, and differentiation, were conducted to study the cytocompatibility of scaffolds. Compressive tests of scaffolds revealed an apparent elastic modulus range of 1.93–5.24 GPa and an ultimate strength ranging within 44.9–237.5 MPa, which were influenced by irregularity and porosity, and improved by heat treatment. Furthermore, the in vitro assay suggested that the original surface of the SLM-fabricated scaffolds was favorable for osteoblasts adhesion and migration because of micro scale pores and ravines. The trabecular-like porous scaffolds with full irregularity and higher porosity exhibited enhanced cells proliferation and osteoblast differentiation at earlier time, due to their preferable combination of small and large pores with various shapes. This study suggested that selective laser melting-derived Ti-6Al-4 V scaffold with the trabecular-like porous structure designed through Voronoi-Tessellation method, favorable mechanical performanc 展开更多
关键词 IRREGULAR porous structure Selective laser melting Voronoi-Tessellation Mechanical performance In VITRO study
酚磺乙胺注射液抑制家兔体外凝血的新发现 预览
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作者 黄惠梅 黄桂连 +7 位作者 陈钧 武氏缘 赵先玲 潘淑芳 朱春燕 韦国榕 丁玄妆 黄勤 《海峡药学》 2019年第5期18-19,共2页
目的酚磺乙胺注射液通常从静脉给药,对患者的止血作用迅速,本文探讨其在体外用药的效应。方法健康家兔3只,由颈动脉放血,在试管中观察凝血时间。分为空白对照组、酚磺乙胺组,其中空白对照组给予生理盐水,酚磺乙胺组给予酚磺乙胺注射液,... 目的酚磺乙胺注射液通常从静脉给药,对患者的止血作用迅速,本文探讨其在体外用药的效应。方法健康家兔3只,由颈动脉放血,在试管中观察凝血时间。分为空白对照组、酚磺乙胺组,其中空白对照组给予生理盐水,酚磺乙胺组给予酚磺乙胺注射液,每支试管均加入2滴,然后各管注入1mL血,记录凝血时间。重复实验3次。结果空白组与酚磺乙胺组之间的差异有统计学意义(P<0.05)。结论酚磺乙胺注射液能抑制家兔体外的凝血,可能跟血管内药效相反。 展开更多
关键词 酚磺乙胺 体外 凝血
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A Second Dose of GnRHa in Combination with Luteal GnRH Antagonist May Eliminate Ovarian Hyperstimulation Syndrome in Women with≥30 Follicles Measuring≥11 mm in Diameter on Trigger Day and/or Pre-trigger Peak Estradiol Exceeding 10 000 pg/mL 预览
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作者 Ling DENG Xue-Ian LI +4 位作者 De-sheng YE Christophe Blockeel Xing-yu ZHOU Shi-ling CHEN Xin CHEN 《当代医学科学(英文)》 SCIE CAS 2019年第2期278-284,共7页
This observational study included 21 patients at remarkably high risk of ovarian hyperstimulation syndrome(OHSS),characterized by more than 30 follicles measuring≥11 mm in diameter on trigger day and/or pre-trigger p... This observational study included 21 patients at remarkably high risk of ovarian hyperstimulation syndrome(OHSS),characterized by more than 30 follicles measuring≥11 mm in diameter on trigger day and/or pre-trigger peak estradiol exceeding 10 000 pg/mL.which was also the feature of women with established severe early OHSS followed by gonadotrophin-releasing hormone agonist(GnRHa)trigger and freeze-all policy that previously have been reported.All patients received a second dose of GnRHa 12 h after the first GnRHa trigger combined with administration of GnRH antagonist at 0.25 mg/day for a period of 3 days from the day of oocyte retrieval onwards.The in vitro fertilization(IVF)outcomes may be preferable compared with a bolus of GnRHa trigger and none of the included patients developed moderate-to-severe OHSS.Moreover,patients'symptoms,reproductive honnone levels and ultrasound findings were improved significantly.This new strategy seems to be efficacious and could be a further supplement of GnRHa trigger with or without applying freeze-all strategy to completely prevent early-onset moderate to severe OHSS,especially for the patients characterized by≥30 follicles measuring≥11 mm in diameter on trigger day and/or pre-trigger peak estradiol exceeding 10 000 pg/mL.Further studies should be performed to compare this regimen with conventional methods of OHSS prevention. 展开更多
关键词 in-vitro fertilization ovarian HYPERSTIMULATION syndrome GNRH AGONIST GNRH ANTAGONIST freeze-all
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In vitro antilithidtic activity of the hydro-alcoholic extract of Cinnamomum zeylanicum Blume bark on calcium oxalate crystallization
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作者 Sana Zaki Nasreen Jahan +1 位作者 Mohd Kalim Ghausia Islam 《结合医学学报:英文版》 CAS CSCD 2019年第4期273-281,共9页
Objective: The study examines the effect of the hydro-alcoholic extract of Cinnamomum zeylanicum Blume bark on crystallization of calcium oxalate. Methods: The antilithiatic effect of various concentrations of the hyd... Objective: The study examines the effect of the hydro-alcoholic extract of Cinnamomum zeylanicum Blume bark on crystallization of calcium oxalate. Methods: The antilithiatic effect of various concentrations of the hydro-alcoholic extract of C. zeylanicum was investigated at various stages of stone formation, using Cystone as a stan da rd refere nee drug. The effect on calcium oxalate crystallization was evaluated by measuring the change in turbidity over time, during crystal nucleation, growth and aggregation, in the metastable solution of calcium chloride and sodium oxalate. The slope from the change in turbidity over time was measured using a spectrophotometer at 620 and 214 nm in respective tests. The inhibition rate was estimated by comparing turbidity in the presence and absence of extract. Crystals formed under experimental conditions were observed under a light microscope, and number and shape of the crystals were assessed in a randomly selected field. Phytochemical analysis and high-performance thin-layer chromatography of the extract was also carried out. Results: C. zeylanicum significantly reduced crystal nucleation at concentrations of 4, 8 and 10 mg/mL (P< 0.001). The inhibition percentage of crystal growth was between 28.30% and 92.46% in the presence of C. zeylanicum extract and from 20.76% to 64.15% with various concentrations of Cystone. The maximum inhibition of crystal growth was obtained from C. zeylanicum at 2 mg/mL (92.46%). Microscopic examination revealed a reduction in the number and size of crystals. In the aggregation assay, the inhibition percentage of C. zeylanicum was between 16.27% and 100%, while Cystone was from -214.68% to 100% at different concentrations. The highest (100%) inhibition of aggregation was found at 4 mg/mL of both the test and standard drugs. Conclusion: We found that C. zeylanicum hydro-alcoholic extract has notable inhibitory effects on various stages of crystallization, in terms of turbidity of solution, as well as the crystal size, number and morphology. 展开更多
关键词 Antilithiatic ACTIVITY Calcium OXALATE Inorganic inhibitors In VITRO UNANI medicine
Effect of preparation program on maternal anxiety of mothers fertilized through in vitro fertilization
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作者 Atefe Salimi Akin Abadi Mitra Zandi +2 位作者 Marzieh Shiva Azita Pourshirvani Anoshirvan Kazemnejad 《亚太生殖杂志(英文版)》 2019年第3期102-111,共10页
Objective: To determine the effect of preparation to play a maternal role in mothers fertilized through in vitro fertilization on maternal anxiety. Methods: A total of 60 mothers undergoing in vitro fertilization were... Objective: To determine the effect of preparation to play a maternal role in mothers fertilized through in vitro fertilization on maternal anxiety. Methods: A total of 60 mothers undergoing in vitro fertilization were assigned into intervention group and control group. Pregnancy concerns and stress questionnaires were research tools. Questionnaires were completed in both groups before organizing preparation program. Then, the preparation program was held for four sessions for the mothers in the intervention group, and the subjects in both groups were immediately investigated again after completion of preparation program and one month later. Data analyses about pregnancy stress and concerns of mothers and its dimensions were performed by repeated measure (analysis of variance), Mann-Whitney, Friedman and Wilcoxon tests. Analysis of demographic variables was performed by using independent t-test and Chi-square test in SPSS 21. Results: The score of pregnancy stress and concerns before the intervention was not significantly different between the two groups. There was a significant difference in the intervention group in the score of pregnancy stress and concerns before and after the intervention (P<0.001), before and one month after the intervention (P<0.001), immediately after and one month after the intervention (P<0.001) which was not significant in the control group. Conclusions: Maternal preparation program can be effective in reducing maternal stress and concerns. 展开更多
关键词 In VITRO FERTILIZATION ANXIETY INFERTILITY PREPARATION NURSING
Association between serum estradiol level on the human chorionic gonadotrophin administration day and clinical outcome
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作者 Xin Li Cheng Zeng +3 位作者 Jing Shang Sheng Wang Xue-Lian Gao Qing Xue 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第10期1194-1201,共8页
Background: Estradiol, as an important hormone in follicular development and endometrial receptivity, is closely related to clinical outcomes of fresh in vitro fertilization embryo transfer (IVF-ET) cycles. The aim of... Background: Estradiol, as an important hormone in follicular development and endometrial receptivity, is closely related to clinical outcomes of fresh in vitro fertilization embryo transfer (IVF-ET) cycles. The aim of this retrospective study was to evaluate the association between elevated serum estradiol (E2) levels on the day of human chorionic gonadotrophin (hCG) administration and IVF-ET pregnancy and birth outcomes. Methods: A total of 1771 infertile patients with their first fresh IVF-ET cycles were analyzed retrospectively between January 2011 and January 2016 in Peking University First Hospital. Patients were categorized by serum E2 levels on the day of hCG administration into six groups: group 1 (serum E2 levels < 1000 pg/mL, n = 205), group 2 (serum E2 levels 1001-2000 pg/mL, n = 457), group 3 (serum E2 levels 2001-3000 pg/mL, n = 425), group 4 (serum E2 levels 3001-4000 pg/mL, n = 310), group 5 (serum E2 levels 4001- 5000 pg/mL, n = 237), and group 6 (serum E2 levels > 5000 pg/mL, n — 137). The retrieved oocyte and Mil oocyte numbers and implantation and clinical pregnancy rates of the groups were compared as the first objective of the study. For the 360 women with singleton births among all patients, the area under the corresponding receiver operating characteristic curve (ROC curve) was calculated to assess the predictive value of the E2 change for the probability of low birth weight (LBW) infants as the second objective. Results: The retrieved oocyte and Mil oocyte numbers and implantation and clinical pregnancy rates gradually increased from groups 1 to 5 but decreased in group 6. The parameters of group 1 were statistically worse than those of the other groups, from group 2 to group 6 (the number of retrieved oocytes, t = 13.096, t = 23.307, t = 23.086, t = 26.376, t = 19.636, P < 0.003;the number of retrieved Mil oocytes, t = 10.856, t = 20.868, t = 21.874, t = 23.374, t = 19.092, P < 0.003;the implantation rate,χ^2= 12.179,χ^2= 22.239,χ^2= 23.993,χ^2= 23.344,χ^2= 16.758, P < 0.003;the cl 展开更多
关键词 ESTRADIOL in VITRO FERTILIZATION Clinical PREGNANCY rate Low BIRTH weight
REplication jumping duration PCRAmplification,Closing and Sanger sequencing of a fragment Of the temptation gene in black-spotted frog1 预览
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作者 Xindi Cui 《探索-动物科学》 2019年第1期16-19,共4页
During in vitro replication of DNA by DNA Polymerase,The diverse palindromic StructuresOn templates of DNA can lead to replication jumping and cause fragments to be missing from daughter strands.We recorded replicatio... During in vitro replication of DNA by DNA Polymerase,The diverse palindromic StructuresOn templates of DNA can lead to replication jumping and cause fragments to be missing from daughter strands.We recorded replication jumping in which a 564 bp fragment was missing from daughter DNA.The original template was a 1126 bp fragment of the introduction of the temporary gene of black-spotted frog(Pelophylax nigromaculatus).The replication jump was recorded after amplifica-tion of this template using polymerase chainREaction,Closing Using Vector,And sequencing using the Sanger Method.The replication jump was elevated to rise from this series of events. 展开更多
关键词 RELOADING DNA polymerase REPLICATION vitro processes FRAGMENT REPLICATION jump
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Hyodeoxycholic acid protects the neurovascular unit against oxygen-glucose deprivation and reoxygenation-induced injury in vitro 预览
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作者 Chang-Xiang Li Xue-Qian Wang +3 位作者 Fa-Feng Cheng Xin Yan Juan Luo Qing-Guo Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1941-1949,共9页
Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin an... Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin and jas-minoidin, HDCA prevents hypoxia-reoxygenation-induced brain injury by suppressing endoplasmic reticulum stress-mediated apoptotic signaling. However, the effects of HDCA in ischemic stroke injury have not yet been studied. Neurovascular unit(NVU) dysfunction occurs in ischemic stroke. Therefore, in this study, we investigated the effects of HDCA on the NVU under ischemic conditions in vitro. We co-cultured primary brain microvascular endothelial cells, neurons and astrocytes using a transwell chamber co-culture system. The NVU was pre-treated with 10.16 or 2.54 μg/mL HDCA for 24 hours before exposure to oxygen-glucose deprivation for 1 hour. The cell counting kit-8 assay was used to detect cell activity. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling were used to assess apoptosis. Enzyme-linked immunosorbent assay was used to measure the expression levels of inflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor-α, and neurotrophic factors, including brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. Oxidative stress-related factors, such as superoxide dismutase, nitric oxide, malondialdehyde and γ-glutamyltransferase, were measured using kits. Pretreatment with HDCA significantly decreased blood-brain barrier permeability and neuronal apoptosis, significantly increased transendothelial electrical resistance and γ-glutamyltransferase activity, attenuated oxidative stress damage and the release of inflammatory cytokines, and increased brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression. Our findings suggest that HDCA maintains NVU morphological integrity and function by modulating inflammation, oxidation stress, apoptosis, and the expression o 展开更多
关键词 hyodeoxycholic acid oxygen glucose deprivation and REOXYGENATION blood-brain barrier permeability anti-oxidative anti-inflammatory ANTI-APOPTOTIC BRAIN-DERIVED NEUROTROPHIC FACTOR glial cell line-derived NEUROTROPHIC FACTOR ischemic stroke in vitro NEUROVASCULAR unit
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Orchestrating stem cell fate: Novel tools for regenerative medicine 预览
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作者 Sara Cruciani Sara Santaniello +2 位作者 Andrea Montella Carlo Ventura Margherita Maioli 《世界干细胞杂志:英文版(电子版)》 2019年第8期464-475,共12页
Mesenchymal stem cells are undifferentiated cells able to acquire different phenotypes under specific stimuli. In vitro manipulation of these cells is focused on understanding stem cell behavior, proliferation and plu... Mesenchymal stem cells are undifferentiated cells able to acquire different phenotypes under specific stimuli. In vitro manipulation of these cells is focused on understanding stem cell behavior, proliferation and pluripotency. Latest advances in the field of stem cells concern epigenetics and its role in maintaining self-renewal and differentiation capabilities. Chemical and physical stimuli can modulate cell commitment, acting on gene expression of Oct-4, Sox-2 and Nanog, the main stemness markers, and tissue-lineage specific genes. This activation or repression is related to the activity of chromatin-remodeling factors and epigenetic regulators, new targets of many cell therapies. The aim of this review is to afford a view of the current state of in vitro and in vivo stem cell applications, highlighting the strategies used to influence stem cell commitment for current and future cell therapies. Identifying the molecular mechanisms controlling stem cell fate could open up novel strategies for tissue repairing processes and other clinical applications. 展开更多
关键词 STEM cells EPIGENETICS SELF-RENEWAL In VITRO differentiation Physical stimuli STEM CELL FATE Clinical practice CELL transplantation
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