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《中华医学杂志:英文版》 CSCD

作品数14078被引量5683H指数12
《中华医学杂志:英文版》
本刊是中国科协主管、中华医学会主办的基础性、临床性和高科技学术期刊,重点报道我国医学各学科最新进展和高水平科研成果,以及临床各科诊疗新经验,是我国医学与世界交流的重要窗口。主要栏目有述评、论著、论著摘...查看详情>>
  • 主办单位中华医学会
  • 国际标准连续出版物号0366-6999
  • 国内统一连续出版物号11-2154/R
  • 出版周期半月刊
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Schistosoma japonicum: construction of phage display antibody library and its application in the immunodiagnosis of infection 认领 被引量:1
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作者 陈代雄 何蔼 +6 位作者 詹希美 俞慕华 雷智刚 孟锦绣 李卓雅 梁瑜 张瑞琳 《中华医学杂志:英文版》 SCIE CAS CSCD 2004年第11期 1697-1703,共7页
Background A monoclonal antibody would be an effective tool for the detection of circulating antigens in the serum of patients with schistosomiasis, but the traditional way of producing monoclonal antibodies is not co... Background A monoclonal antibody would be an effective tool for the detection of circulating antigens in the serum of patients with schistosomiasis, but the traditional way of producing monoclonal antibodies is not cost-effective. The objective of this study was to find a new method for the large-scale production of monoclonal antibodies against Schistosoma japonicum (Sj).Methods A phage display antibody library for Sj was constructed. To obtain a single-chain variable fragment antibody (scFv) against Sj, the library was screened with metabolic antigens from adult Sj worms (Sj-MAg) using enzyme-linked immunosorbent assay. The soluble scFvs selected were used to detect Sj antigens in the serum of acute and chronic schistosomiasis patients.Results Six positive clones with good reactivity to Sj-MAg were obtained from the phage display antibody library of about 1.07×106 individual clones. Only two of these six clones bound specifically to Sj-MAg and were chosen for further analysis. Specific soluble anti-Sj-MAg scFvs were produced by inducing the 2 clones with isopropyl-D-thiogalactopyranoside. The characteristics of the scFvs were then determined. The results of Western blot showed that these scFvs could bind to Sj-MAg specifically and had a molecular weight of about 31 kD. When testing serum from schistosomiasis patients with one of the two specific scFvs, its sensitivity was found to be 60% and 37% in acute and chronic patients, respectively, with a specificity of 90%. When the two specific scFvs were combined, their sensitivity was found to be 75% and 57% in acute and chronic patients, respectively, with a specificity of 85%.Conclusions The results indicate that the scFvs are potentially useful for the diagnosis of schistosomiasis. The library construction also provides a useful tool for the further screening of other antibodies for both diagnostic and immunotherapeutic applications and for epitope analysis and vaccine design. 展开更多
关键词 日本血吸虫属 抗菌素 抗体 免疫诊断 传染病 单链可变受体 血清学诊断
Clinical features and management of postural tachycardia syndrome in children: a single-center experience 认领
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作者 Li Jiawei Zhang Qingyou +2 位作者 Hao Hongjun Jin Hongfang Du Junbao 《中华医学杂志:英文版》 SCIE CAS CSCD 2014年第21期3684-3689,共6页
关键词 临床特征 综合征 儿童 体位 过速 特征和 北京大学第一医院 去甲肾上腺素
Prevalence and Prescription of Antidepressants in Depression with Somatic Comorbidity in Asia: The Research on East Asian Psychotropic Prescription Patterns Study 认领
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作者 Chao Chen Tian-Mei Si +13 位作者 Yu-Tao Xiang Gabor S Ungvari Chuan-Yue Wang Yan-Ling He Ee-Heok Kua Senta Fuji Kang Sim Jitendra K Trivedi Eun-Kee Chung Pichet Udomratn Kok-Yoon Chee Norman Sartorius Chay-Hoon Tan Naotaka Shinfuku 《中华医学杂志:英文版》 SCIE CAS CSCD 2015年第7期853-858,共6页
关键词 抗抑郁药 亚洲国家 精神药物 患病率 处方 东亚 去甲肾上腺素 抑郁症
Serum Hepcidin Predicts Uremic Accelerated Atherosclerosis in Chronic Hemodialysis Patients with Diabetic Nephropathy 认领 被引量:2
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作者 Han Li Su-Juan Feng +3 位作者 Lu-Lu Su Wei Wang Xiao-Dong Zhang Shi-Xiang Wang 《中华医学杂志:英文版》 SCIE CAS CSCD 2015年第10期1351-1357,共7页
关键词 糖尿病肾病 动脉粥样硬化 血液透析 血清铁 尿毒症 加速性 HEPCIDIN 液相色谱-串联质谱法
Cancer Incidence and Mortality Survey in Wuwei, Gansu Province, Northwestern China from 2003 to 2012: A Retrospective Population-based Study 认领
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作者 Cheng-Yun Li Yan-Cheng Ye +7 位作者 Ge-Yu Liang Wen-Hua Zhang Zhi-Yi Zhang Xiao-Qin Liu Ying Liang Fen-Lan Xu Jing Li Ji-Lian Xiang 《中华医学杂志:英文版》 SCIE CAS CSCD 2016年第6期636-644,共9页
关键词 癌症发病率 死亡率 甘肃省 武威 中国标准 世界标准 中枢神经系统 结直肠癌
Cystic Bronchiectasis Imitating Pulmonary Aspergilloma 认领
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作者 Hong-Xia Shao 《中华医学杂志:英文版》 SCIE CAS CSCD 2016年第17期2137-2138,共2页
To the Editor:A 65-year-old female patient presented with the complaints of hemoptysis and the left upper chest pain for about 1 week.The medical history revealed coronary heart disease and paroxysmal atrial fibrillat... To the Editor:A 65-year-old female patient presented with the complaints of hemoptysis and the left upper chest pain for about 1 week.The medical history revealed coronary heart disease and paroxysmal atrial fibrillation,smoking for about 40 years,no drinking,and no special contact history.There was a prior history of chronic cough without dyspnea or hemoptysis for about 20 years. 展开更多
关键词 支气管 菌球 模拟 呼吸困难 阵发性 冠心病 咳嗽
Clinical Application of the UK Working Party's Criteria for the Diagnosis of Atopic Dermatitis in the Chinese Population by Age Group 认领
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作者 Li Wang Lin-Feng Li 《中华医学杂志:英文版》 SCIE CAS CSCD 2016年第23期2829-2833,共5页
关键词 临床诊断 临床应用 工作组 英国 年龄 KAPPA值 皮炎 分组
Abscess of Zygomatic Root: A Rare Otogenic Complication 认领
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作者 Yao Qin Tian-Cheng Li +1 位作者 Tie-Chuan Cong Yu-He Liu 《中华医学杂志:英文版》 SCIE CAS CSCD 2017年第6期749-750,共2页
A 10-year-old Girl with Metastatic Unclassified Sarcoma with Epithelioid Features 认领
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作者 Zheng-Xiu Li Song Zheng +4 位作者 Hang-Hang Jiang Yu-Zhe Sun Rui-Qun Qi Yu-Xiao Hong Xing-Hua Gao 《中华医学杂志:英文版》 SCIE CAS CSCD 2017年第11期1385-1386,共2页
A Novel Mutation of PRKAR1A Caused Carney Complex in a Chinese Patient 认领
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作者 Xiao-Ling Cai Jing Wu +3 位作者 Ying-Ying Luo Ling Chen Xue-Yao Han Li-Nong Ji 《中华医学杂志:英文版》 SCIE CAS CSCD 2017年第24期3009-3010,共2页
Inductively Coupled Plasma Mass Spectrometry as a Reference Method to Evaluate Serum Calcium Measurement Bias and the Commutability of Processed Materials during Routine Measurements 认领
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作者 Hai-Jian Zhao Meng-Lei Ge +6 位作者 Yin Yan Tian-Jiao Zhang Jie Zeng Wei-Yan Zhou Yu-Fei Wang Qing-Hui Meng Chuan-Bao Zhang 《中华医学杂志:英文版》 SCIE CAS CSCD 2018年第13期1584-1590,共7页
关键词 材料 浆液 偏爱 测量 评估 血浆 诱导 样品准备
Association of Cytokines with Alanine Aminotransferase, Hepatitis B Virus Surface Antigen and Hepatitis B Envelope Antigen Levels in Chronic Hepatitis B 认领
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作者 Ming-Hui Li Yao Lu +16 位作者 Lu Zhang Xing-Yue Wang Chong-Ping Ran Hong-Xiao Hao Dan Zhang Xiao-Jing Qu Ge Shen Shu-Ling Wu Wei-Hua Cao Tian-Lin Qi Ru-Yu Liu Lei-Ping Hu Min Chang Wen-Hao Hua Shun-Ai Liu Gang Wan Yao Xie 《中华医学杂志:英文版》 SCIE CAS CSCD 2018年第15期1813-1818,共6页
关键词 CYTOKINES 表面抗原 丙氨酸 肝炎 CYTOKINES HBV-DNA 病毒 信封
Perinatal complications and live-birth outcomes following assisted reproductive technology: a retrospective cohort study 认领
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作者 Ling-Ling Lei Yong-Lian Lan +2 位作者 Shu-Yu Wang Wei Feng Zhi-Jin Zhai 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第20期2408-2416,共9页
Background:With the development of assisted reproductive technology(ART)and its increasing success rate in the mainland of China,more attention has been paid to the safety of ART.In this study,we explored the associat... Background:With the development of assisted reproductive technology(ART)and its increasing success rate in the mainland of China,more attention has been paid to the safety of ART.In this study,we explored the associations between conception by ART and pregnancy/perinatal complications,and neonatal outcomes compared with similar outcomes following spontaneous conception.Methods:This retrospective cohort study of pregnancies over a 3-year period(2013-2015)was performed at Beijing Obstetrics and Gynecology Hospital,Beijing,China.Subjects were divided into two groups:conception by ART(n=2256)or spontaneous conception(n=6768).According to different fertilization modes,the ART group was divided into in vitro fertilization(IVF,n=1873)and intracytoplasmic sperm injection(ICSI,n=383)subgroups.The ART group was also divided into two different embryo transfer methods;fresh embryo transfer(ET,n=1583)and frozen embryo transfer(FET,n=673)subgroups.Pregnancy complications,perinatal complications,and neonatal outcomes of the enrolled subjects were investigated and analyzed by univariate analysis and multivariate logistic regression.Results:After adjustment for maternal age,gravidity,parity,maternal education,smoking,alcohol consumption,and body mass index(BMI),pregnancies conceived by ART were associated with a significantly increased incidence of gestational diabetes mellitus(GDM;OR 1.88,95%CI 1.56-2.27),gestational hypertension(OR 2.18,95%CI 1.83-2.60),and intrahepatic cholestasis of pregnancy(ICP)(OR 2.79,95%CI 2.15-3.64),compared with spontaneous conception.These associations were similar for the singleton group.In the twin group,only the incidence of ICP was significantly higher than in controls.We found that pregnancies conceived by ART were associated with perinatal complications,including placental abruption(OR 2.14,95%CI 1.33-3.45),premature rupture of membranes(PROM;OR 1.24,95%CI 1.06-1.45),postpartum hemorrhage(OR 2.89,95%CI 2.33-3.59)and polyhydramnios(OR 2.01,95%CI 1.29-3.16).The singleton group had a similar result 展开更多
关键词 REPRODUCTIVE techniques Assisted FERTILIZATION in VITRO SPERM injections Intracytoplasmic Pregnancy complications
支气管哮喘患儿IL—4近侧启动子区克隆和多态性分析 认领
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作者 周玉峰 付劲蓉 +1 位作者 吴健民 李成荣 《中华医学杂志:英文版》 SCIE CAS CSCD 2002年第11期 1624-1627,共4页
目的克隆和研究支气管哮喘患儿白细胞介素-4(IL-4)近侧启动子区DNA序列的多态性. 方法取40名有明显家族及过敏史的哮喘患儿和10名正常儿童,多聚酶链式反应(PCR)结合单链构象多态性(SSCP )扩增、筛选IL-4近侧启动子片段,进一步构建正常... 目的克隆和研究支气管哮喘患儿白细胞介素-4(IL-4)近侧启动子区DNA序列的多态性. 方法取40名有明显家族及过敏史的哮喘患儿和10名正常儿童,多聚酶链式反应(PCR)结合单链构象多态性(SSCP )扩增、筛选IL-4近侧启动子片段,进一步构建正常对照和异常条带PCR产物的重组质粒pIL-4-Jx2,并用双脱氧链终止法对重组质粒进行序列测定. 结果在40名患儿SSCP分析中发现了7组异常条带.DNA测序结果显示有四处变异位点位于已知的调控元件之内或毗邻位点: 一名病人-229位C被A所替代,该变异恰好位于IL-4正性调节元件-Ⅰ(PRE-Ⅰ)元件之内;两名病人负性调节元件-Ⅱ(NRE-Ⅱ)元件毗邻C被T所替代, TATA框前增加一个G;一名病人STAT-6元件附近缺少了ATTTT五碱基核苷酸.结论过敏性哮喘患儿IL-4近侧启动子区DNA序列存在多态性,这可能与IL-4基因异常表达及哮喘的发病有关. 展开更多
关键词 支气管哮喘 白介素4 启动子 分子克隆 序列测定 儿童
晚期肝癌介入治疗综合措施的探讨 认领 被引量:1
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作者 曹喜才 贺能树 +9 位作者 孙建中 王松 吉训明 范海伦 王金胜 张长林 杨建国 吕提文 李建华 张国欣 《中华医学杂志:英文版》 SCIE CAS CSCD 2002年第12期 1883-1885,共3页
目的探讨晚期肝癌介入治疗综合措施,进一步提高临床疗效.方法对33例肝癌介入治疗晚期综合症状采取一系列治疗措施.21例行经皮股动脉药盒导管系统(PCS)植入术;出血应用凝血酶原复合物;肝性脑病应用支链氨基酸和颈精氨酸等综合措施.结果 2... 目的探讨晚期肝癌介入治疗综合措施,进一步提高临床疗效.方法对33例肝癌介入治疗晚期综合症状采取一系列治疗措施.21例行经皮股动脉药盒导管系统(PCS)植入术;出血应用凝血酶原复合物;肝性脑病应用支链氨基酸和颈精氨酸等综合措施.结果 21例PCS 术后留置管全部保持通畅.33例平均生存期20.1个月,2年生存率为36%.结论对肝癌介入治疗晚期采取一系列综合措施可延长患者生存期,提高生存质量,是肝癌介入治疗上一个新台阶. 展开更多
关键词 晚期肝癌 介入治疗 经皮股动脉药盒导管系统植入术
早期糖尿病视网膜病变的多焦视网膜电图及临床意义 认领 被引量:1
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作者 余敏忠 张欣 +4 位作者 钟兴武 于强 江福钿 马娟妹 吴德正 《中华医学杂志:英文版》 SCIE CAS CSCD 2002年第4期 563-566,共4页
目的研究早期糖尿病视网膜病变的多焦视网膜电图特征及其临床意义.方法采用VERIS Ⅳ视觉诱发反应图象系统测量和分析糖尿病视网膜病变Ⅰ-Ⅱ期34眼的多焦视网膜电图改变.结果在直径约45度的测试范围内6个离心度的环状区和4个象限,Ⅰ-Ⅱ... 目的研究早期糖尿病视网膜病变的多焦视网膜电图特征及其临床意义.方法采用VERIS Ⅳ视觉诱发反应图象系统测量和分析糖尿病视网膜病变Ⅰ-Ⅱ期34眼的多焦视网膜电图改变.结果在直径约45度的测试范围内6个离心度的环状区和4个象限,Ⅰ-Ⅱ期糖尿病视网膜病变与正常对照眼比较,N1波、P1波和N2波的反应密度绝对值以及N1-P1和P1-N2反应密度峰-峰值显著降低.结论在糖尿病视网膜病变发展过程中多焦视网膜电图反应密度已经出现异常,多焦视网膜电图作为一种客观定量的视觉空间功能检查新方法,对糖尿病视网膜病变的具有早期诊断的价值. 展开更多
关键词 糖尿病视网膜病变 多焦视网膜电图 视野 离心度
Expression of C5aR (CD88) of synoviocytes isolated from patients with rheumatoid arthritis and osteoarthritis 认领
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作者 袁国华 魏锦 +3 位作者 周京国 胡宏 唐中 张国元 《中华医学杂志:英文版》 SCIE CAS CSCD 2003年第9期 1408-1412,共5页
Objective To investigate the expression of anaphylatoxin receptor C5aR (CD88) in synoviocytes from patients with rheumatoid arthritis (RA) and osteoarthritis (OA).Methods The expression of C5aR was assessed in synovio... Objective To investigate the expression of anaphylatoxin receptor C5aR (CD88) in synoviocytes from patients with rheumatoid arthritis (RA) and osteoarthritis (OA).Methods The expression of C5aR was assessed in synoviocytes isolated from 27 RA and 12 OA patients using reverse transcription-polymerase chain reactions (RT-PCR), flow cytometry, and immunofluorescence analysis. The effects of C5a on the release of tumor necrosis factor α (TNFα) from synoviocytes were assayed using enzyme-linked immunosorbent assays (ELISA).Results C5aR mRNA was detected in 24 of 27 samples from RA patients, and 10 of 12 samples from OA patients. Flow cytometric analysis and immunofluorescence study demonstrated the cell surface expression of C5aR in a significant proportion of synoviocytes from both RA and OA patients, and the level of C5aR expression in synoviocytes was significantly correlated with joint swelling, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) in RA patients. Finally, interaction of C5aR with its ligand C5a was shown to enhance lipopolysaccharide (LPS)-induced TNFα release from synoviocytes.Conclusions The expression of C5aR in synoviocytes from inflammatory joint diseases and also the induction of TNFα release in activated synovicytes by the interaction of C5a and C5aR suggest that the C5a/C5aR system may play an important role in joint inflammation process. 展开更多
关键词 风湿性关节炎 骨关节炎 C5AR 滑膜细胞
Retroperitoneoscopic renal pedicle lymphatic disconnection for chyluria 认领 被引量:1
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作者 江军 朱方强 +2 位作者 靳凤烁 姜庆 王洛夫 《中华医学杂志:英文版》 SCIE CAS CSCD 2003年第11期 1746-1748,共3页
Objective To report our experiences in retroperitonescopic renal pedicle lymphatic stripping for chyluria. Methods Six cases of filarial chyluria were admitted to our hospital from November 2001 to June 2002. Of these... Objective To report our experiences in retroperitonescopic renal pedicle lymphatic stripping for chyluria. Methods Six cases of filarial chyluria were admitted to our hospital from November 2001 to June 2002. Of these cases, 4 were men and 2 women, with age ranging from 34 to 52 years (mean, 42 years). Diagnosis was made by using urine test for the presence of chyle and fat globule, cystoscopy, excretory urogram and retrograde pyelography. Chyluria was found on the left renal unit in 2 cases and on the right side in 4 cases. The technique of retroperitoneoscopic management of chyluria consisted of nephrolympholysis, hilar vessel stripping and ureterolympholysis.Results Operative time ranged from 69 to 120 minutes (mean, 95 minutes). Intraoperative blood loss was 50-180 ml (mean, 85 ml). Chyluria disappeared in all patients immediately after operation. Mild hematuria occurred in 4 cases within 12 hours and disappeared at 24 hours. Subcutaneous emphysema around the lesions was found in 2 cases and was spontaneously absorbed 3 days after the treatment. There was no lymphatic leak at the lesions. The patients were discharged from the hospital 5-9 days after the treatment. All patients gained weight and their haemoglobin and serum protein increased by 13.5 g/L and 3.66 g/L respectively. No chyluria recurrence was reported during 1-1.6 years follow-up. Conclusions Retroperitoneoscopic renal pedicle lymphatic disconnection for chyluria is a safe, effective and efficient surgical procedure with minimal invasion, less pain, lower morbidity, short hospital stay and rapid recovery. 展开更多
关键词 乳糜尿 腹腔镜手术 微创外科 临床应用
Transcription factor HAND2 mutations in sporadic Chinese patients with congenital heart disease 认领 被引量:1
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作者 SHEN Lei LI Xiao-feng +5 位作者 SHEN A-dong WANG Qiang LIU Cai-xia GUO Ya-jie SONG Zhen-jiang LI Zhong-zhi 《中华医学杂志:英文版》 SCIE CAS CSCD 2010年第13期1623-1627,共5页
基本 helix-loop-helix 抄写因素 HAND2 玩的背景在心脏的 morphogenesis.However 的一个必要角色,在同族的心疾病(CHD ) 和在 HAND2 遗传型和 CHD 显型之间的关联的 HAND2 变化的流行没广泛地被学习。我们在 131 个病人放大了 exons ... 基本 helix-loop-helix 抄写因素 HAND2 玩的背景在心脏的 morphogenesis.However 的一个必要角色,在同族的心疾病(CHD ) 和在 HAND2 遗传型和 CHD 显型之间的关联的 HAND2 变化的流行没广泛地被学习。我们在 131 个病人放大了 exons 和 HAND2 基因的 flanking intron 序列的方法与正确的室,流出道,大动脉的动脉或心脏的垫子的先天的缺点诊断了并且由 sequencing.Results 证实了变化包括三个错误感觉变化( P11R , S36N 和 V83L )的七个变化,一个 isonymous 变化( H14H )和在 untranslated 区域的三个变化( 241 A > G , 604C > T 和 3237T >一)从 131 个 patients.Both nonisonymous 变化在 12 被识别是 l 仅仅一个变化(S36N ) 在 250 正常健康控制被识别。3637T 的分发 > A 是是 2 groups.Conclusions HAND2 可以是的 differentbetween 的唯一的正确的室的狭窄的潜在的候选人基因,流出道。有 HAND2 变化的家庭历史的那些的进一步的学习将信服地帮助联系他们的遗传型到 CHD 的致病。 展开更多
关键词 先天性心脏病 基因突变 转录因子 患者 动脉血管 非编码区 转录激活 候选基因
Catheter ablation of paroxysmal atrial fibrillation in a young patient with a partial anomalous pulmonary venous connection 认领
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作者 HUANG He YANG Bo JIANG Hong WU Gang HUANG Cong-xin 《中华医学杂志:英文版》 SCIE CAS CSCD 2010年第16期2284-2286,共3页
关键词 肺静脉 阵发性 颤动 心房 患者 导管 引流 PAF
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