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褪黑素对急性百草枯中毒大鼠的治疗效果 认领 被引量:2

Use of melatonin in rats with acute paraquat poisoning
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摘要 目的观察褪黑素(MT)对急性百草枯(PQ)中毒大鼠的治疗效果。方法选择成年SD大鼠54只,随机分为正常对照组、染毒组和治疗组(每组18只)。正常对照组用生理盐水灌胃,染毒组用PQ一次性灌胃(50mg/kg)染毒,治疗组在染毒后随即腹腔注入MT10mg/kg,1次/d。分别于1d、3d和7d测定3组大鼠血清中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH—Px)的活力,并观察大鼠中毒表现及肺组织病理改变。结果PQ染毒后大鼠血清中MDA含量显著高于对照组(P〈0.01);而血清SOD及GSH—Px活力明显低于对照组(P〈0.05)。与染毒组比较,治疗组大鼠血清MDA含量下降;GSH—Px和SOD活力逐渐升高,差异具有统计学意义(P〈0.05)。其中毒表现及肺组织病理学改变均明显减轻。结论MT可减轻急性PQ中毒引起的脂质过氧化反应,改善大鼠的中毒症状。 Objective To investigate the effects of melatonin (MT) on rats with acute paraquat (PQ) poisoning. Method Fifty-four Sprague-Dawley (SD) rats were randomly divided into three groups (each group 18 rats) and given the following treatment: intragastric injection of PQ at 50 mg/kg (PQ); intragastric injection of pamquat followed by intraperitoneal injection of MT at 10mg/kg once a day (MT) ; intragastric injection of normal saline (Control). Serum assays for malondialdehyde (MDA) levels and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were determined on the 1st, 3rd and 7th day post treatment. Clinical manifestations of poisoning and pathological changes in the lungs were also observed. Results Serum MDA levels were significantly increased ( P 〈 0.01 ), and SOD and GSH-Px activities significantly decreased ( P 〈 0.05) in the PQ group compared to the control group. Serum MDA levels were significantly decreased, and serum SOD and GSH-Px activities increased in MT group compared to the PQ group ( P 〈 0.05), Clinical manifestations of intoxication and pathological lung changes were also ameliorated in poisoned rats treated with MT. Conclutions Administration of MT alleviates chnical manifestations of acute paraquat poisoning in rats by hmiting the damage from lipid perexidation.
作者 郭宪立 佟飞 田英平 石汉文 霍书花 扈琳 陈慧 刘力斗 高恒波 王霞 GUO Xian-li, TONG Fei, TIAN Ying-ping, SHI Han-wen, HUO Shu-hua, HU Lin, CHEN Hui, LIU Li-dou, GAO Hem-bo, WANG Xia. (Department of Emergency, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China)
出处 《中华急诊医学杂志》 CAS CSCD 2008年第9期948-951,共4页 Chinese Journal of Emergency Medicine
基金 河北省科技攻关项目(052761501)
关键词 中毒 百草枯 褪黑索 治疗 Poisoning Paraquat Melatonin Therapy
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参考文献8

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